Leafdata Category Search

Gene expression

ROT3P::GUS ... in leaves, histochemical analysis indicated that the ... expression ... was ubiquitous in all cell layers, including epidermis, palisade tissue, and the spongy layer

Kim GT, Tsukaya H, Saito Y, Uchimiya H - Changes in the shapes of leaves and flowers upon overexpression of cytochrome P450 in Arabidopsis

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Gene expression

ROT3P::GUS ... expression ... was ... in ... the spongy layer

Kim GT, Tsukaya H, Saito Y, Uchimiya H - Changes in the shapes of leaves and flowers upon overexpression of cytochrome P450 in Arabidopsis

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Gene expression

ROT3P::GUS ... we observed ... in leaves

Kim GT, Tsukaya H, Saito Y, Uchimiya H - Changes in the shapes of leaves and flowers upon overexpression of cytochrome P450 in Arabidopsis

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Gene expression

ROT3P::GUS ... expression ... was ... in ... epidermis

Kim GT, Tsukaya H, Saito Y, Uchimiya H - Changes in the shapes of leaves and flowers upon overexpression of cytochrome P450 in Arabidopsis

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Gene expression

in leaves, histochemical analysis indicated that the ROT3 promoter was expressed more strongly in distal, mature regions than in proximal, immature regions

Kim GT, Tsukaya H, Saito Y, Uchimiya H - Changes in the shapes of leaves and flowers upon overexpression of cytochrome P450 in Arabidopsis

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Gene expression

ROT3P::GUS ... expression ... was ... in ... palisade tissue

Kim GT, Tsukaya H, Saito Y, Uchimiya H - Changes in the shapes of leaves and flowers upon overexpression of cytochrome P450 in Arabidopsis

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Gene expression

YAB3 is detected in the abaxial regions of the developing leaves

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

YAB2 also appears to be expressed abaxially in cotyledon primordia

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

FIL ... expression extending through more than half of the cell layers in leaf primordia

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

FIL, YAB2 and YAB3 are expressed in a qualitatively similar manner at the mRNA level, although expression levels differ dramatically ... FIL is expressed at a high level, YAB3 at a moderate level, and YAB2 at a low level

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

FIL ... Expression is initially in a small group of subepidermal cells in the central region of leaf anlagen

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

In most leaf primordia of phb-1d homozygotes, FIL expression is undetectable

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

FIL ... expressed in abaxial regions of above ground lateral organ primordia

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

YAB2 ... expressed in abaxial regions of above ground lateral organ

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

FIL is initially expressed in the transition-stage embryo (between the late globular and heart stages) in a small number of subepidermal cells in the central region of the cotyledon anlagen

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

In leaves, FIL is more broadly ... expressed

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

In phb-1d/+ heterozygotes ... FIL expression domain is reduced, usually consisting of only a couple of cell layers on the abaxial side of the leaf primordium (Fig. 5B), rather than in four cell layers as observed in wild type

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

in developing embryos ... initial detection of YAB3 is at the early heart stage

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

YAB3 ... expressed in abaxial regions of above ground lateral organ primordia

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

YAB3 ... Expression ... appears to be in ... three layers of spongy mesophyll

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

FIL ... No signal is detected in differentiated leaves

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

in leaf primordium ... As the central cells begin differentiating, FIL expression declines, but remains high in the marginal abaxial regions

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

In differentiating leaves FIL mRNA can be detected ... in layers destined to become spongy mesophyll

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

In differentiating leaves FIL mRNA can be detected in the abaxial epidermis

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

FIL, YAB2 and YAB3 are expressed in a qualitatively similar manner at the mRNA level, although expression levels differ dramatically ... FIL is expressed at a high level, YAB3 at a moderate level, and YAB2 at a low level

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

YAB3 ... expressed in abaxial regions of ... cotyledons

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

Expression of YAB2 ... is detected in the abaxial regions of the developing leaves

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

In differentiating leaves FIL mRNA ... in the ... adaxial epidermis expression ... is undetectable

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

In phb-1d/+ heterozygotes FIL expression is detected in most leaf primordia

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

FIL ... expression is throughout the abaxial domain of the cotyledon ... primordia ... walking-stick ... stages

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

YAB2 ... expressed in abaxial regions of ... cotyledons

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

FIL ... expressed in abaxial regions of ... cotyledons

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

FIL ... expression is throughout the abaxial domain of the cotyledon primordia ... U ... - ... shaped stages

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

As leaves fully differentiate, YAB3 expression declines to an undetectable level

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

YAB3 expression ... with initial expression occurring in leaf anlagen

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

FIL ... expressed in abaxial regions of ... leaves

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

FIL ... As leaf primordia emerge, expression becomes restricted to the abaxial regions

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

FIL ... By mid-heart stage expression is throughout the abaxial domain of the cotyledon primordia

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

In differentiating leaves FIL mRNA ... in the palisade mesophyll ... expression ... is undetectable

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

YAB2 ... expressed in abaxial regions of ... leaves

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

FIL, YAB2 and YAB3 are expressed in a qualitatively similar manner at the mRNA level, although expression levels differ dramatically ... FIL is expressed at a high level, YAB3 at a moderate level, and YAB2 at a low level

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

YAB3 ... in developing embryos ... expressed abaxially in cotyledon primordia

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

FIL expression is detectable in at least two incipient leaf primordia prior to their emergence from the flanks of the apical meristem

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

FIL ... gradually fades as the embryos mature

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

FIL expression is not altered in ... leaves ... of yab3-1 mutants

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

YAB3 ... Expression appears to be in ... the abaxial epidermis

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

YAB3 ... expressed in abaxial regions of ... leaves

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

YAB3 ... As leaf primordia emerge from the apical meristem, expression is confined to the abaxial regions of developing primordia

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

FIL ... By early heart stage expression has expanded to include cells on the abaxial side of the emergent cotyledon primordia, but does not immediately extend to the tip of the cotyledon primordia

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

FIL ... expression is throughout the abaxial domain of the cotyledon ... primordia ... torpedo ... stages

Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis

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Gene expression

CycD3 transcripts were still observed in 35S::ANT leaves, but not in control

Mizukami Y, Fischer RL - Plant organ size control: AINTEGUMENTA regulates growth and cell numbers during organogenesis

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Gene expression

ANT mRNA accumulated in leaf

Mizukami Y, Fischer RL - Plant organ size control: AINTEGUMENTA regulates growth and cell numbers during organogenesis

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Gene expression

We estimate ... ≈35-fold more AtEXP10 mRNA in young petioles as compared with corresponding mature tissues

Cho HT, Cosgrove DJ - Altered expression of expansin modulates leaf growth and pedicel abscission in Arabidopsis thaliana

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Gene expression

As leaf development progressed, AtEXP10::GUS expression began in the base of the petiole and gradually extended toward the whole midrib

Cho HT, Cosgrove DJ - Altered expression of expansin modulates leaf growth and pedicel abscission in Arabidopsis thaliana

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Gene expression

The first expression detectable by AtEXP10::GUS occurred at the base of the emerging first two true leaves but not of the cotyledons

Cho HT, Cosgrove DJ - Altered expression of expansin modulates leaf growth and pedicel abscission in Arabidopsis thaliana

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Gene expression

AtEXP10::GUS ... low level of staining in the shoot meristem was observed

Cho HT, Cosgrove DJ - Altered expression of expansin modulates leaf growth and pedicel abscission in Arabidopsis thaliana

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Gene expression

AtEXP10::GUS expression ... disappeared altogether as the leaf matured

Cho HT, Cosgrove DJ - Altered expression of expansin modulates leaf growth and pedicel abscission in Arabidopsis thaliana

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Gene expression

AtEXP10::GUS expression ... later ... was restricted to the vasculature of the petiole and leaf blade

Cho HT, Cosgrove DJ - Altered expression of expansin modulates leaf growth and pedicel abscission in Arabidopsis thaliana

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Gene expression

AtEXP10::GUS also was notably expressed in growing trichomes

Cho HT, Cosgrove DJ - Altered expression of expansin modulates leaf growth and pedicel abscission in Arabidopsis thaliana

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Gene expression

We estimate ≈200-fold more AtEXP10 mRNA in young leaf blades ... as compared with corresponding mature tissues

Cho HT, Cosgrove DJ - Altered expression of expansin modulates leaf growth and pedicel abscission in Arabidopsis thaliana

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Gene expression

LEP expression was significantly increased in let compared to wild type

van der Graaff E, Dulk-Ras AD, Hooykaas PJ, Keller B - Activation tagging of the LEAFY PETIOLE gene affects leaf petiole development in Arabidopsis thaliana

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Gene expression

LEP is only expressed in young shoots of wild-type plants

van der Graaff E, Dulk-Ras AD, Hooykaas PJ, Keller B - Activation tagging of the LEAFY PETIOLE gene affects leaf petiole development in Arabidopsis thaliana

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Gene expression

In wild type no LEP expression could be detected in the cotyledons

van der Graaff E, Dulk-Ras AD, Hooykaas PJ, Keller B - Activation tagging of the LEAFY PETIOLE gene affects leaf petiole development in Arabidopsis thaliana

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Gene expression

detected LEP expression in stipules

van der Graaff E, Dulk-Ras AD, Hooykaas PJ, Keller B - Activation tagging of the LEAFY PETIOLE gene affects leaf petiole development in Arabidopsis thaliana

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Gene expression

In wild type no LEP expression could be detected in the ... non-expanding (old) leaves

van der Graaff E, Dulk-Ras AD, Hooykaas PJ, Keller B - Activation tagging of the LEAFY PETIOLE gene affects leaf petiole development in Arabidopsis thaliana

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Gene expression

LEP expression ... restricted to the leaf blades

van der Graaff E, Dulk-Ras AD, Hooykaas PJ, Keller B - Activation tagging of the LEAFY PETIOLE gene affects leaf petiole development in Arabidopsis thaliana

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Gene expression

LEP expression was strongest in very young leaf blades and became weaker upon further expansion of these blades

van der Graaff E, Dulk-Ras AD, Hooykaas PJ, Keller B - Activation tagging of the LEAFY PETIOLE gene affects leaf petiole development in Arabidopsis thaliana

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Gene expression

In let ... LEP expression was detected in all organs analysed

van der Graaff E, Dulk-Ras AD, Hooykaas PJ, Keller B - Activation tagging of the LEAFY PETIOLE gene affects leaf petiole development in Arabidopsis thaliana

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Gene expression

In wild type, LEP was expressed throughout very young leaf primordia

van der Graaff E, Dulk-Ras AD, Hooykaas PJ, Keller B - Activation tagging of the LEAFY PETIOLE gene affects leaf petiole development in Arabidopsis thaliana

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Gene expression

LEP was very weakly expressed in wild type and could only be detected in young shoots

van der Graaff E, Dulk-Ras AD, Hooykaas PJ, Keller B - Activation tagging of the LEAFY PETIOLE gene affects leaf petiole development in Arabidopsis thaliana

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Gene expression

LEP ... staining in all cells constituting the young leaf primordia

van der Graaff E, Dulk-Ras AD, Hooykaas PJ, Keller B - Activation tagging of the LEAFY PETIOLE gene affects leaf petiole development in Arabidopsis thaliana

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Gene expression

In ... 35SDE-LEP overexpressors LEP expression was detected in all organs analysed

van der Graaff E, Dulk-Ras AD, Hooykaas PJ, Keller B - Activation tagging of the LEAFY PETIOLE gene affects leaf petiole development in Arabidopsis thaliana

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Gene expression

LEP expression could also be detected in young developing leaves

van der Graaff E, Dulk-Ras AD, Hooykaas PJ, Keller B - Activation tagging of the LEAFY PETIOLE gene affects leaf petiole development in Arabidopsis thaliana

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Gene expression

clf-18 ... leaves ... contain ... AP3 floral genes

Serrano-Cartagena J, Candela H, Robles P, Ponce MR, Pérez-Pérez JM, Piqueras P, Micol JL - Genetic analysis of incurvata mutants reveals three independent genetic operations at work in Arabidopsis leaf morphogenesis

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Gene expression

icu2 leaves ... contain ... AG ... floral genes

Serrano-Cartagena J, Candela H, Robles P, Ponce MR, Pérez-Pérez JM, Piqueras P, Micol JL - Genetic analysis of incurvata mutants reveals three independent genetic operations at work in Arabidopsis leaf morphogenesis

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Gene expression

clf-18 ... leaves ... contain ... AG ... floral genes

Serrano-Cartagena J, Candela H, Robles P, Ponce MR, Pérez-Pérez JM, Piqueras P, Micol JL - Genetic analysis of incurvata mutants reveals three independent genetic operations at work in Arabidopsis leaf morphogenesis

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Gene expression

Ectopic transcription of AP1 was detected in ... clf-61 allele

Serrano-Cartagena J, Candela H, Robles P, Ponce MR, Pérez-Pérez JM, Piqueras P, Micol JL - Genetic analysis of incurvata mutants reveals three independent genetic operations at work in Arabidopsis leaf morphogenesis

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Gene expression

Ectopic transcription of AP1 was detected in icu2

Serrano-Cartagena J, Candela H, Robles P, Ponce MR, Pérez-Pérez JM, Piqueras P, Micol JL - Genetic analysis of incurvata mutants reveals three independent genetic operations at work in Arabidopsis leaf morphogenesis

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Gene expression

icu2 leaves ... contain ... AP3 floral genes

Serrano-Cartagena J, Candela H, Robles P, Ponce MR, Pérez-Pérez JM, Piqueras P, Micol JL - Genetic analysis of incurvata mutants reveals three independent genetic operations at work in Arabidopsis leaf morphogenesis

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Gene expression

Using in situ hybridization ... SHOOTMERISTEMLESS (STM) ... as1 ... se ... strong expression in the meristem

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

Using in situ hybridization, we could not detect SHOOTMERISTEMLESS (STM) expression in leaves of as1 ... se

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

KNAT1 ... as1 ... se ... expression was confined to the peripheral zone

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

as1 se ... showed expression of KNAT1::GUS in leaves

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

KNAT2::GUS ... Leaves of ... as2 ... revealed GUS expression in the sinuses

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

KNAT2::GUS expression appeared normal in se mutants

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

KNAT1::GUS-19 expression in pkl-15 single mutants was indistinguishable from that of wild type

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

as2 se ... showed expression of KNAT1::GUS in leaves

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

KNAT1::GUS-18 ... Weak GUS expression was also seen in the sinus of the occasional lobes that formed on as1 ... leaves

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

In as2 mutants, KNAT1::GUS-1 expression was detected in the petioles of leaves

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

KNAT1::GUS-18 ... Weak GUS expression was also seen in the sinus of the occasional lobes that formed on ... as2 leaves

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

Sporadic expression of KNAT1 was detected in the basal part of young leaves of ... as2 se mutants

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

KNAT1 ... as2 ... se ... expression was confined to the peripheral zone

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

Sporadic expression of KNAT1 was detected in the basal part of young leaves of ... as2

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

In as2 mutants, KNAT1::GUS-1 expression was detected ... in the main vein

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

Expression of KNAT1 RNA in meristems of ... as1 ... se ... appeared similar to that of wild- type plants

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

In as2 mutants, KNAT1::GUS-1 expression was detected ... sometimes in secondary veins

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

KNAT1::GUS-19 ... GUS expression in as1-1 pkl-15 plants was ... less intense throughout the leaf

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

KNAT1::GUS-19 ... GUS expression in as1-1 pkl-15 plants was concentrated in the sinus region

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

KNAT1 ... as1 ... expression was confined to the peripheral zone

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

KNAT1::GUS-1 line was detected in the ... shoot meristems

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

Sporadic expression of KNAT1 was detected in the basal part of young leaves of as1

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

KNAT1 ... as2 ... expression was confined to the peripheral zone

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

Expression of KNAT1 RNA in meristems of ... as1 ... appeared similar to that of wild- type plants

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

KNAT1 ... as1 ... se ... not detected ... in the youngest leaf primordia

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

KNAT2::GUS ... Leaves of ... as2 single mutants revealed GUS expression ... slightly in the basal portion of the leaf petioles

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

Using in situ hybridization ... SHOOTMERISTEMLESS (STM) ... as1 ... strong expression in the meristem

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

Using in situ hybridization, we could not detect SHOOTMERISTEMLESS (STM) expression in leaves of as1

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

Expression of KNAT1 RNA in meristems of ... as2 ... se ... appeared similar to that of wild- type plants

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

Using in situ hybridization ... SHOOTMERISTEMLESS (STM) ... as2 ... strong expression in the meristem

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

Using in situ hybridization, we could not detect SHOOTMERISTEMLESS (STM) expression in leaves of ... se

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

as2 se ... showed expression of KNAT1::GUS in leaves

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

Using in situ hybridization, we could not detect SHOOTMERISTEMLESS (STM) expression in leaves of ... as2

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

KNAT1::GUS-18 ... expression was strong ... in the vasculature of as1 leaves

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

KNAT2::GUS ... Leaves of as1 ... single mutants revealed GUS expression ... slightly in the basal portion of the leaf petioles

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

as2 se double mutants expressed KNAT2::GUS strongly in the sinuses of lobes

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

KNAT1 ... as2 ... not detected ... in the youngest leaf primordia

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

Using in situ hybridization ... SHOOTMERISTEMLESS (STM) ... as2 ... se ... strong expression in the meristem

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

In wild-type plants carrying the KNAT2::GUS construct, staining was detected in meristems but not leaves

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

KNAT2::GUS ... Leaves of as1 ... revealed GUS expression in the sinuses

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

as1 se ... double mutants expressed KNAT2::GUS strongly in the sinuses of lobes

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

Using in situ hybridization ... SHOOTMERISTEMLESS (STM) ... se ... strong expression in the meristem

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

KNAT1::GUS-1 line was detected ... not in leaves

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

Using in situ hybridization, we could not detect SHOOTMERISTEMLESS (STM) expression in leaves of ... as2 ... se

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

KNAT1 ... as1 ... not detected ... in the youngest leaf primordia

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

Expression of KNAT1 RNA in meristems of ... as2 ... appeared similar to that of wild- type plants

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

In wild-type plants carrying the KNAT2::GUS construct, staining was detected in meristems

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

KNAT1 ... as2 ... se ... not detected ... in the youngest leaf primordia

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

In as2 mutants, KNAT1::GUS-1 expression was detected ... occasionally in patches on the leaves

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

KNAT1::GUS-18 ... expression was strong in the petioles ... of as1 leaves

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

Sporadic expression of KNAT1 was detected in the basal part of young leaves of ... as1 se

Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot

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Gene expression

transgenic lines in which NAC1 was ... silenced ... DBP ... showed ... a reduction

Xie Q, Frugis G, Colgan D, Chua NH - Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development

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Gene expression

auxin treatment increased ... AIR3 expression levels in wild-type plants

Xie Q, Frugis G, Colgan D, Chua NH - Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development

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Gene expression

Whole-mount in situ hybridization of 7–12-d-old seedlings detected ... tissues actively expressing the NAC1 gene ... the primordia of the developing leaves

Xie Q, Frugis G, Colgan D, Chua NH - Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development

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Gene expression

transgenic lines in which NAC1 was ... silenced ... AIR3 ... showed ... a reduction

Xie Q, Frugis G, Colgan D, Chua NH - Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development

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Gene expression

transgenic lines carrying a GFP-NAC1 fusion gene expressed under the control of a dexamethasone-inducible GVG promoter ... In the absence of dex, no fluorescence was observed (Fig. (Fig.2A,2A, panel 2), however, in the presence of dex, the GFP–NAC1 was localized exclusively to the nucleus

Xie Q, Frugis G, Colgan D, Chua NH - Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development

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Gene expression

NAC1 was expressed at a moderate level in 2-wk-old seedlings, however, in dissected tissues of mature plants it was expressed at high levels in roots, at low levels in stems and leaves, and not at all in flowers or siliques

Xie Q, Frugis G, Colgan D, Chua NH - Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development

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Gene expression

transgenic lines in which NAC1 was overexpressed ... AIR3 ... showed an increase

Xie Q, Frugis G, Colgan D, Chua NH - Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development

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Gene expression

Whole-mount in situ hybridization of 7–12-d-old seedlings detected ... tissues actively expressing the NAC1 gene: the expanding cotyledons

Xie Q, Frugis G, Colgan D, Chua NH - Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development

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Gene expression

auxin treatment increased ... NAC1 ... expression levels in wild-type plants

Xie Q, Frugis G, Colgan D, Chua NH - Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development

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Gene expression

construct containing a 2-kb DNA fragment upstream of the NAC1 coding region was fused to a β-glucuronidase (GUS) reporter gene, and several independent transgenic lines were analyzed ... In the leaf primordia, a signal, which diminished as the leaf matured, was also observed

Xie Q, Frugis G, Colgan D, Chua NH - Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development

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Gene expression

a construct containing a 2-kb DNA fragment upstream of the NAC1 coding region was fused to a β-glucuronidase (GUS) reporter gene, and several independent transgenic lines were analyzed ... moderate activity was observed in the cotyledons

Xie Q, Frugis G, Colgan D, Chua NH - Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development

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Gene expression

transgenic lines in which NAC1 was overexpressed ... DBP ... showed an increase

Xie Q, Frugis G, Colgan D, Chua NH - Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development

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Gene expression

35S-ICK1 ... Expression of the CDK gene cdc2a did not decrease, and perhaps showed a slight increase

Wang H, Zhou Y, Gilmer S, Whitwill S, Fowke LC - Expression of the plant cyclin-dependent kinase inhibitor ICK1 affects cell division, plant growth and morphology

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Gene expression

KNAT1 was ectopically expressed in as1 leaves

Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis

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Gene expression

KNAT1 transcripts in wild-type plants were detected ... not in leaves

Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis

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Gene expression

KNAT2 transcripts were present at high levels in wild-type shoot

Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis

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Gene expression

as1 showed misexpression of KNAT1

Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis

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Gene expression

In wild-type embryos AS1 RNA ... was absent or reduced in ... the cotyledon epidermis

Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis

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Gene expression

STM expression ... is absent from ... leaf primordia ... in as1

Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis

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Gene expression

as1 showed misexpression of ... KNAT2

Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis

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Gene expression

wild-type embryos AS1 RNA ... was absent or reduced in cells that would subsequently form the shoot apical meristem

Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis

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Gene expression

STM expression in the wild type ... is absent from ... leaf primordia

Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis

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Gene expression

In wild-type embryos AS1 RNA first became detectable in late globular stage, predominantly in two subepidermal domains corresponding to cotyledon initials

Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis

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Gene expression

After germination, AS1 was detected in leaf founder cells ... until stage P4

Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis

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Gene expression

KNAT2 transcripts were present ... at a low level in wild-type leaves

Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis

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Gene expression

In wild-type embryos AS1 RNA ... Expression was maintained in subepidermal cells of developing cotyledons from heart stage onwards

Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis

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Gene expression

After germination, AS1 was detected ... in cells associated with the cotyledon vasculature

Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis

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Gene expression

KNAT1 transcripts in wild-type plants were detected in whole-shoot

Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis

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Gene expression

STM expression in the wild type ... is absent from leaves

Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis

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Gene expression

in as1 ... STM expression was unchanged

Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis

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Gene expression

STM expression ... is absent from leaves ... in as1

Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis

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Gene expression

STM expression in the wild type is confined to SAM cells

Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis

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Gene expression

KNAT1 ... was ectopically expressed in the cotyledons of as1 embryos

Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis

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Gene expression

STM ... expression ... is confined to SAM cells ... in as1

Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis

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Gene expression

In as1 mutant leaves, expression of KNAT2 was upregulated

Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis

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Gene expression

stm embryos revealed AS1 expression throughout the apical half of ... heart stage embryos

Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis

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Gene expression

the expression pattern of STM does not change in as1 mutants

Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis

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Gene expression

After germination, AS1 was detected in leaf founder cells from the time of primordium initiation

Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis

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Gene expression

expression of ATβ-Amy ... in plants grown on media lacking sugars did not differ between 35S::ATHB13 plants and wild type

Hanson J, Johannesson H, Engström P - Sugar-dependent alterations in cotyledon and leaf development in transgenic plants expressing the HDZhdip gene ATHB13

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Gene expression

expression of ATβ-Amy ... was induced to higher levels in 35S::ATHB13 plants than in the wild type

Hanson J, Johannesson H, Engström P - Sugar-dependent alterations in cotyledon and leaf development in transgenic plants expressing the HDZhdip gene ATHB13

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Gene expression

expression of ... VSP was induced to higher levels in 35S::ATHB13 plants than in the wild type

Hanson J, Johannesson H, Engström P - Sugar-dependent alterations in cotyledon and leaf development in transgenic plants expressing the HDZhdip gene ATHB13

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Gene expression

CHS ... not affected by ATHB13 over-expression

Hanson J, Johannesson H, Engström P - Sugar-dependent alterations in cotyledon and leaf development in transgenic plants expressing the HDZhdip gene ATHB13

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Gene expression

expression of ... VSP ... in plants grown on media lacking sugars did not differ between 35S::ATHB13 plants and wild type

Hanson J, Johannesson H, Engström P - Sugar-dependent alterations in cotyledon and leaf development in transgenic plants expressing the HDZhdip gene ATHB13

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Gene expression

KNAT1 ... transcripts were detected in the peripheral zones ... in the wild type

Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves

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Gene expression

wild-type leaves did not accumulate any detectable STM transcripts

Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves

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Gene expression

as1-1 ... levels of KNAT1 ... transcripts ... were higher than in the wild type

Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves

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Gene expression

KNAT1 transcripts ... as2-1 ... were detected ... not in the ... presumptive leaf initials

Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves

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Gene expression

KNAT1 transcripts ... were detected ... not in the ... presumptive leaf initials ... in the wild type

Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves

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Gene expression

Levels of STM transcripts in as2- 1 rosette leaves fluctuated

Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves

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Gene expression

In the rosette leaves of as1 ... levels of transcripts of the KNAT6 gene increased

Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves

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Gene expression

KNAT1 transcripts ... were detected in the ... basal regions of leaf primordia ... in the wild type

Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves

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Gene expression

KNAT1 transcripts ... as2-1 ... were detected ... not in the ... small leaf primordia

Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves

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Gene expression

KNAT1 transcripts ... were detected ... not in the central zones ... in the wild type

Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves

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Gene expression

as2-1 ... levels of KNAT1 transcripts ... were higher than in the wild type

Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves

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Gene expression

When primers for amplification of KNAT1 cDNA were used, products of PCR were detected in samples from all the leaves ... of ... as2 ... but not of wild-type plants

Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves

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Gene expression

KNAT1 transcripts ... as2-1 ... were detected in the ... basal regions of leaf primordia

Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves

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Gene expression

KNAT1 ... we occasionally detected weak but significant hybridization signals in some as2-1 leaf primordia and such signals were not detected in the wild type

Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves

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Gene expression

No significant increase in the accumulation of WUS transcripts was detected in as1-1 ... rosette leaves

Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves

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Gene expression

Transcripts of the STM gene accumulated in the first and second rosette leaves of as1-1 plants

Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves

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Gene expression

When primers for amplification of KNAT1 cDNA were used, products of PCR were detected in samples from all the leaves ... of as1 ... but not of wild-type plants

Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves

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Gene expression

The pattern of accumulation of KNAT1 transcripts in the as1-1 meristem was also similar to that in the wild type

Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves

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Gene expression

No significant increase in the accumulation of WUS transcripts was detected in ... as2-1 rosette leaves

Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves

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Gene expression

KNAT1 ... we detected relatively strong hybridization signals at the bases of as2-1 leaf primordia

Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves

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Gene expression

KNAT1 transcripts ... were detected ... not in the ... small leaf primordia ... in the wild type

Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves

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Gene expression

When we used primers for KNAT2 cDNA, increased levels of products were detected in ... as2

Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves

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Gene expression

When we used primers for KNAT2 cDNA, increased levels of products were detected in ... as1

Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves

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Gene expression

KNAT6 ... in the wild type, transcripts of this gene accumulated in the shoot apices

Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves

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Gene expression

In situ hybridization showed that the pattern of accumulation of KNAT1 transcripts around vegetative meristems of as2-1 was similar to that of the wild type

Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves

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Gene expression

KNAT1 ... as2-1 ... transcripts were detected in the peripheral zones

Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves

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Gene expression

In the rosette leaves of ... as2 ... levels of transcripts of the KNAT6 gene increased

Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves

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Gene expression

KAN ... soon thereafter becomes restricted to peripheral cells in a basal region of the embryo

Kerstetter RA, Bollman K, Taylor RA, Bomblies K, Poethig RS - KANADI regulates organ polarity in Arabidopsis

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Gene expression

Translational fusions between the KAN cDNA and ... b-glucuronidase (GUS ... KAN directed reporter gene expression to the nucleus

Kerstetter RA, Bollman K, Taylor RA, Bomblies K, Poethig RS - KANADI regulates organ polarity in Arabidopsis

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Gene expression

KAN ... In heart-stage embryos, expression was observed both in the periphery of the presumptive hypocotyl

Kerstetter RA, Bollman K, Taylor RA, Bomblies K, Poethig RS - KANADI regulates organ polarity in Arabidopsis

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Gene expression

KAN is weakly expressed throughout the early globular embryo

Kerstetter RA, Bollman K, Taylor RA, Bomblies K, Poethig RS - KANADI regulates organ polarity in Arabidopsis

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Gene expression

During vegetative growth, KANmRNAwas observed ... not in older leaves

Kerstetter RA, Bollman K, Taylor RA, Bomblies K, Poethig RS - KANADI regulates organ polarity in Arabidopsis

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Gene expression

During vegetative growth, KANmRNAwas observed on the abaxial side of very young leaf primordia

Kerstetter RA, Bollman K, Taylor RA, Bomblies K, Poethig RS - KANADI regulates organ polarity in Arabidopsis

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Gene expression

KAN ... In heart-stage embryos, expression was observed ... on the abaxial side of cotyledon primordia

Kerstetter RA, Bollman K, Taylor RA, Bomblies K, Poethig RS - KANADI regulates organ polarity in Arabidopsis

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Gene expression

Translational fusions between the KAN cDNA and ... modified green fluorescent protein (mGFP5 ... KAN directed reporter gene expression to the nucleus

Kerstetter RA, Bollman K, Taylor RA, Bomblies K, Poethig RS - KANADI regulates organ polarity in Arabidopsis

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Gene expression

The highest levels of PHB RNA are in adaxial (or central) regions of the embryo

McConnell JR, Emery J, Eshed Y, Bao N, Bowman J, Barton MK - Role of PHABULOSA and PHAVOLUTA in determining radial patterning in shoots

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Gene expression

PHB transcript is initially present throughout the presumptive cotyledons

McConnell JR, Emery J, Eshed Y, Bao N, Bowman J, Barton MK - Role of PHABULOSA and PHAVOLUTA in determining radial patterning in shoots

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Gene expression

PHB expression in the meristem are missing in phb-1d mutants

McConnell JR, Emery J, Eshed Y, Bao N, Bowman J, Barton MK - Role of PHABULOSA and PHAVOLUTA in determining radial patterning in shoots

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Gene expression

The PHB transcript is preferentially expressed in the adaxial domain of the developing leaf

McConnell JR, Emery J, Eshed Y, Bao N, Bowman J, Barton MK - Role of PHABULOSA and PHAVOLUTA in determining radial patterning in shoots

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Gene expression

PHB ... globular stage just before cotyledon outgrowth) is seen at high levels in their adaxial domains

McConnell JR, Emery J, Eshed Y, Bao N, Bowman J, Barton MK - Role of PHABULOSA and PHAVOLUTA in determining radial patterning in shoots

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Gene expression

Thus, in young primordia, the unit accumulating PHB transcript is the adaxial leaf domain and adjacent meristem cells

McConnell JR, Emery J, Eshed Y, Bao N, Bowman J, Barton MK - Role of PHABULOSA and PHAVOLUTA in determining radial patterning in shoots

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Gene expression

To visualize the deduced decrease in cell division, we analyzed a mitotic reporter (19, 20) in the gpa1 background. b-Glucuronidase (GUS) staining of both the apical meristems and basal cells of the first leaf was markedly reduced in gpa1 mutants compared with con- trols

Ullah H, Chen JG, Young JC, Im KH, Sussman MR, Jones AM - Modulation of cell proliferation by heterotrimeric G protein in Arabidopsis

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Gene expression

GPA1 expression in actively dividing cells

Ullah H, Chen JG, Young JC, Im KH, Sussman MR, Jones AM - Modulation of cell proliferation by heterotrimeric G protein in Arabidopsis

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Gene expression

The levels of BAS1 transcripts were not detectable in dwf4

Choe S, Fujioka S, Noguchi T, Takatsuto S, Yoshida S, Feldmann KA - Overexpression of DWARF4 in the brassinosteroid biosynthetic pathway results in increased vegetative growth and seed yield in Arabidopsis

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Gene expression

The levels of BAS1 transcripts were ... decreased in bri1

Choe S, Fujioka S, Noguchi T, Takatsuto S, Yoshida S, Feldmann KA - Overexpression of DWARF4 in the brassinosteroid biosynthetic pathway results in increased vegetative growth and seed yield in Arabidopsis

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Gene expression

DET2 ... transcript levels were increased in the ... bri1 mutant plants

Choe S, Fujioka S, Noguchi T, Takatsuto S, Yoshida S, Feldmann KA - Overexpression of DWARF4 in the brassinosteroid biosynthetic pathway results in increased vegetative growth and seed yield in Arabidopsis

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Gene expression

CPD transcript levels were increased in the ... bri1 mutant plants

Choe S, Fujioka S, Noguchi T, Takatsuto S, Yoshida S, Feldmann KA - Overexpression of DWARF4 in the brassinosteroid biosynthetic pathway results in increased vegetative growth and seed yield in Arabidopsis

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Gene expression

CPD transcript levels were increased in the dwf4 ... mutant plants

Choe S, Fujioka S, Noguchi T, Takatsuto S, Yoshida S, Feldmann KA - Overexpression of DWARF4 in the brassinosteroid biosynthetic pathway results in increased vegetative growth and seed yield in Arabidopsis

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Gene expression

overexpresses the DWF4 gene ... The level of TCH4 was significantly increased in AOD4 compared to both wild-type and dwarf mutants

Choe S, Fujioka S, Noguchi T, Takatsuto S, Yoshida S, Feldmann KA - Overexpression of DWARF4 in the brassinosteroid biosynthetic pathway results in increased vegetative growth and seed yield in Arabidopsis

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Gene expression

DET2 ... transcript levels were increased in the dwf4 ... mutant plants

Choe S, Fujioka S, Noguchi T, Takatsuto S, Yoshida S, Feldmann KA - Overexpression of DWARF4 in the brassinosteroid biosynthetic pathway results in increased vegetative growth and seed yield in Arabidopsis

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Gene expression

overexpresses the DWF4 gene ... The levels of BAS1 transcripts were ... slightly increased in AOD4 relative to the wild type

Choe S, Fujioka S, Noguchi T, Takatsuto S, Yoshida S, Feldmann KA - Overexpression of DWARF4 in the brassinosteroid biosynthetic pathway results in increased vegetative growth and seed yield in Arabidopsis

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Gene expression

mRNA of REV ... restricted to the adaxial domain as developing primordia

Eshed Y, Baum SF, Perea JV, Bowman JL - Establishment of polarity in lateral organs of plants

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Gene expression

In kan1 kan2 seedlings, initial FIL expression appears normal

Eshed Y, Baum SF, Perea JV, Bowman JL - Establishment of polarity in lateral organs of plants

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Gene expression

REV ... initiates normally in kan1 kan2 leaf primordia

Eshed Y, Baum SF, Perea JV, Bowman JL - Establishment of polarity in lateral organs of plants

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Gene expression

mRNA of ... PHV ... Later expression is confined to the provascular and vascular tissues of leaves

Eshed Y, Baum SF, Perea JV, Bowman JL - Establishment of polarity in lateral organs of plants

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Gene expression

mRNA of ... PHV is localized to the SAM, throughout leaf primordia anlagen

Eshed Y, Baum SF, Perea JV, Bowman JL - Establishment of polarity in lateral organs of plants

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Gene expression

mRNA of REV ... Later expression is confined to the provascular and vascular tissues of leaves

Eshed Y, Baum SF, Perea JV, Bowman JL - Establishment of polarity in lateral organs of plants

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Gene expression

In wild-type, mRNA of FIL is first detected throughout leaf primordia anlagen

Eshed Y, Baum SF, Perea JV, Bowman JL - Establishment of polarity in lateral organs of plants

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Gene expression

mRNA of ... PHV is localized to the SAM

Eshed Y, Baum SF, Perea JV, Bowman JL - Establishment of polarity in lateral organs of plants

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Gene expression

mRNA of REV ... is localized to the SAM

Eshed Y, Baum SF, Perea JV, Bowman JL - Establishment of polarity in lateral organs of plants

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Gene expression

REV ... in kan1 kan2 leaf primordia ... confinement to the adaxial domain is delayed

Eshed Y, Baum SF, Perea JV, Bowman JL - Establishment of polarity in lateral organs of plants

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Gene expression

PHV ... in kan1 kan2 leaf primordia ... confinement to the adaxial domain is delayed

Eshed Y, Baum SF, Perea JV, Bowman JL - Establishment of polarity in lateral organs of plants

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Gene expression

mRNA of ... PHV is ... restricted to the adaxial domain as developing primordia

Eshed Y, Baum SF, Perea JV, Bowman JL - Establishment of polarity in lateral organs of plants

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Gene expression

kan1 kan2 seedlings ... FIL ... domain is reduced in size. In developing leaf primordia, FIL was not detected in more than 2-3 cell layers, even though these primordia have more cell layers than wild-type

Eshed Y, Baum SF, Perea JV, Bowman JL - Establishment of polarity in lateral organs of plants

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Gene expression

PHV, initiates normally in kan1 kan2 leaf primordia

Eshed Y, Baum SF, Perea JV, Bowman JL - Establishment of polarity in lateral organs of plants

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Gene expression

mRNA of REV ... is localized to the SAM, throughout leaf primordia anlagen

Eshed Y, Baum SF, Perea JV, Bowman JL - Establishment of polarity in lateral organs of plants

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Gene expression

kan1 kan2 ... PHV, levels of mRNA are higher

Eshed Y, Baum SF, Perea JV, Bowman JL - Establishment of polarity in lateral organs of plants

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Gene expression

In wild-type, mRNA of FIL ... becomes confined to the abaxial side of the leaf

Eshed Y, Baum SF, Perea JV, Bowman JL - Establishment of polarity in lateral organs of plants

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Gene expression

Expression of ELK4 (AGB1) was found in all tissues tested

Lease KA, Wen J, Li J, Doke JT, Liscum E, Walker JC - A mutant Arabidopsis heterotrimeric G-protein beta subunit affects leaf, flower, and fruit development

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Gene expression

to determine the level of AtCAP1 in distinct Arabidopsis tissues. After RT-PCR, a specific 410-bp fragment was detected in ... cotyledons

Barrero RA, Umeda M, Yamamura S, Uchimiya H - Arabidopsis CAP regulates the actin cytoskeleton necessary for plant cell elongation and division

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Gene expression

to determine the level of AtCAP1 in distinct Arabidopsis tissues. After RT-PCR, a specific 410-bp fragment was detected in ... shoots

Barrero RA, Umeda M, Yamamura S, Uchimiya H - Arabidopsis CAP regulates the actin cytoskeleton necessary for plant cell elongation and division

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Gene expression

to determine the level of AtCAP1 in distinct Arabidopsis tissues. After RT-PCR, a specific 410-bp fragment was detected in ... leaves

Barrero RA, Umeda M, Yamamura S, Uchimiya H - Arabidopsis CAP regulates the actin cytoskeleton necessary for plant cell elongation and division

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Gene expression

The quantitative estimation of the amount of protein accumulation indicated that although the actin level was maintained constant in all plants, the AtCAP1 level increased as the amount of Dex was increased in transgenic plants (Figure 6B). S4 plants accumulated AtCAP1 protein at high levels, whereas S11 plants accumulated AtCAP1 at low levels

Barrero RA, Umeda M, Yamamura S, Uchimiya H - Arabidopsis CAP regulates the actin cytoskeleton necessary for plant cell elongation and division

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Gene expression

We obtained several transgenic lines possessing a chimeric AtCAP1 gene under a GAL4 promoter, which is recognized by the GVG transcription factor upon activation with dexamethasone (Dex) (Aoyama and Chua, 1997) (Figure 6A) . Three independent transgenic plants, S4, S9, and S11, each with a single copy of AtCAP1 (data not shown) and with different expression levels of the AtCAP1 protein (Figure 6B), were chosen for further analysis

Barrero RA, Umeda M, Yamamura S, Uchimiya H - Arabidopsis CAP regulates the actin cytoskeleton necessary for plant cell elongation and division

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Gene expression

To characterize the level of accumulation of the AtCAP1 protein in Arabidopsis tissues, total proteins from suspension-cultured cells, roots, stems, leaves, and flowers were extracted and subjected to protein gel blot analysis as described in Methods. The AtCAP1 antibody specifically recognized a 52-kD protein, which is the predicted molecular mass of the AtCAP1 protein. A detectable level of AtCAP1 protein was found in suspension-cultured cells and roots

Barrero RA, Umeda M, Yamamura S, Uchimiya H - Arabidopsis CAP regulates the actin cytoskeleton necessary for plant cell elongation and division

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Gene expression

AS1 was expressed in all plant tissues examined: roots, stems, leaves, flowers and siliques, with preferential expression in young and immature plant tissues. In the developed rosette and cauline leaves, AS1 had very low expression

Sun Y, Zhou Q, Zhang W, Fu Y, Huang H - ASYMMETRIC LEAVES1, an Arabidopsis gene that is involved in the control of cell differentiation in leaves

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Gene expression

AtCUL1 is expressed in dividing cells throughout the plant (data not shown) and is localized primarily to the nucleus

del Pozo JC, Dharmasiri S, Hellmann H, Walker L, Gray WM, Estelle M - AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 is required for auxin response

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Gene expression

To further study AXR1 gene expression, we placed the β-glucuronidase reporter gene (GUS) adjacent to the promoter region of the AXR1 gene and introduced this construct into Arabidopsis plants ... Staining also was observed in the ... hydathodes

del Pozo JC, Dharmasiri S, Hellmann H, Walker L, Gray WM, Estelle M - AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 is required for auxin response

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Gene expression

ECR1 expression was observed in ... shoot meristems

del Pozo JC, Dharmasiri S, Hellmann H, Walker L, Gray WM, Estelle M - AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 is required for auxin response

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Gene expression

ECR1 RNA was not detected in mature (nongrowing) tissues of the plant, such as in the stem distal to the apex or in fully expanded leaves

del Pozo JC, Dharmasiri S, Hellmann H, Walker L, Gray WM, Estelle M - AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 is required for auxin response

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Gene expression

To further study AXR1 gene expression, we placed the β-glucuronidase reporter gene (GUS) adjacent to the promoter region of the AXR1 gene and introduced this construct into Arabidopsis plants ... we observed GUS staining in vascular tissues in cotyledons

del Pozo JC, Dharmasiri S, Hellmann H, Walker L, Gray WM, Estelle M - AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 is required for auxin response

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Gene expression

we examined the pattern of AXR1 accumulation during embryogenesis. High levels of AXR1 protein were found in the zygote and throughout embryogenesis (Figures 1M to 1P and data not shown). A similar distribution of AXR1 RNA was observed (data not shown)

del Pozo JC, Dharmasiri S, Hellmann H, Walker L, Gray WM, Estelle M - AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 is required for auxin response

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Gene expression

AXR1 protein was found in the ... leaf primordium

del Pozo JC, Dharmasiri S, Hellmann H, Walker L, Gray WM, Estelle M - AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 is required for auxin response

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Gene expression

To further study AXR1 gene expression, we placed the β-glucuronidase reporter gene (GUS) adjacent to the promoter region of the AXR1 gene and introduced this construct into Arabidopsis plants ... Staining also was observed in the trichomes

del Pozo JC, Dharmasiri S, Hellmann H, Walker L, Gray WM, Estelle M - AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 is required for auxin response

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Gene expression

To further study AXR1 gene expression, we placed the β-glucuronidase reporter gene (GUS) adjacent to the promoter region of the AXR1 gene and introduced this construct into Arabidopsis plants ... we observed GUS staining in vascular tissues in ... mature leaves

del Pozo JC, Dharmasiri S, Hellmann H, Walker L, Gray WM, Estelle M - AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 is required for auxin response

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Gene expression

AXR1 RNA accumulated ... in young leaves

del Pozo JC, Dharmasiri S, Hellmann H, Walker L, Gray WM, Estelle M - AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 is required for auxin response

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Gene expression

AXR1 was found primarily in the nuclei of these cells

del Pozo JC, Dharmasiri S, Hellmann H, Walker L, Gray WM, Estelle M - AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 is required for auxin response

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Gene expression

AXR1 protein was found in the shoot meristem

del Pozo JC, Dharmasiri S, Hellmann H, Walker L, Gray WM, Estelle M - AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 is required for auxin response

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Gene expression

AXR1 RNA accumulated in the root and shoot meristems

del Pozo JC, Dharmasiri S, Hellmann H, Walker L, Gray WM, Estelle M - AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 is required for auxin response

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Gene expression

ECR1 expression was observed in ... embryo

del Pozo JC, Dharmasiri S, Hellmann H, Walker L, Gray WM, Estelle M - AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 is required for auxin response

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Gene expression

AN gene was expressed ... in ... leaves

Kim GT, Shoda K, Tsuge T, Cho KH, Uchimiya H, Yokoyama R, Nishitani K, Tsukaya H - The ANGUSTIFOLIA gene of Arabidopsis, a plant CtBP gene, regulates leaf-cell expansion, the arrangement of cortical microtubules in leaf cells and expression of a gene involved in cell-wall formation

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Gene expression

MERI5 (Medford et al., 1991), was among the genes that were specifically up- regulated in the an mutant ... We found thatMERI5 was expressed at a 3-fold higher level in the an-1 mutant than in the wild type, as indicated by the microarray analysis (3.3-fold higher by microarray analysis; 3.27-fold higher by RT±PCR analy- sis

Kim GT, Shoda K, Tsuge T, Cho KH, Uchimiya H, Yokoyama R, Nishitani K, Tsukaya H - The ANGUSTIFOLIA gene of Arabidopsis, a plant CtBP gene, regulates leaf-cell expansion, the arrangement of cortical microtubules in leaf cells and expression of a gene involved in cell-wall formation

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Gene expression

AN gene was expressed ... in cotyledons

Kim GT, Shoda K, Tsuge T, Cho KH, Uchimiya H, Yokoyama R, Nishitani K, Tsukaya H - The ANGUSTIFOLIA gene of Arabidopsis, a plant CtBP gene, regulates leaf-cell expansion, the arrangement of cortical microtubules in leaf cells and expression of a gene involved in cell-wall formation

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Gene expression

an ... We found no difference in numbers of MTs ... by moni- toring expression of the TUB6::GFP fusion gene

Kim GT, Shoda K, Tsuge T, Cho KH, Uchimiya H, Yokoyama R, Nishitani K, Tsukaya H - The ANGUSTIFOLIA gene of Arabidopsis, a plant CtBP gene, regulates leaf-cell expansion, the arrangement of cortical microtubules in leaf cells and expression of a gene involved in cell-wall formation

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Gene expression

AN gene to a gene for green fluorescent protein (sGFP::S65T) under the control of the constitutively expressed 35S RNA promoter of cauliflower mosaic virus (CaMV ... we detected green fluorescence within the nuclei of cells in stable Arabidopsis transformants

Kim GT, Shoda K, Tsuge T, Cho KH, Uchimiya H, Yokoyama R, Nishitani K, Tsukaya H - The ANGUSTIFOLIA gene of Arabidopsis, a plant CtBP gene, regulates leaf-cell expansion, the arrangement of cortical microtubules in leaf cells and expression of a gene involved in cell-wall formation

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Gene expression

an ... Analysis by RT±PCR on one of the endogenous genes for tubulin, TUB4, indicated that there were no significant differences among the levels of expression of the transcript of this gene

Kim GT, Shoda K, Tsuge T, Cho KH, Uchimiya H, Yokoyama R, Nishitani K, Tsukaya H - The ANGUSTIFOLIA gene of Arabidopsis, a plant CtBP gene, regulates leaf-cell expansion, the arrangement of cortical microtubules in leaf cells and expression of a gene involved in cell-wall formation

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Gene expression

rot3 ... We found no difference in numbers of MTs ... by moni- toring expression of the TUB6::GFP fusion gene

Kim GT, Shoda K, Tsuge T, Cho KH, Uchimiya H, Yokoyama R, Nishitani K, Tsukaya H - The ANGUSTIFOLIA gene of Arabidopsis, a plant CtBP gene, regulates leaf-cell expansion, the arrangement of cortical microtubules in leaf cells and expression of a gene involved in cell-wall formation

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Gene expression

EXGT-A1 ... did not show such differences (not significantly different from the wild type; level of significance, 5%) when we compared an-1 and wild-type plants

Kim GT, Shoda K, Tsuge T, Cho KH, Uchimiya H, Yokoyama R, Nishitani K, Tsukaya H - The ANGUSTIFOLIA gene of Arabidopsis, a plant CtBP gene, regulates leaf-cell expansion, the arrangement of cortical microtubules in leaf cells and expression of a gene involved in cell-wall formation

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Gene expression

rot3 ... Analysis by RT±PCR on one of the endogenous genes for tubulin, TUB4, indicated that there were no significant differences among the levels of expression of the transcript of this gene also

Kim GT, Shoda K, Tsuge T, Cho KH, Uchimiya H, Yokoyama R, Nishitani K, Tsukaya H - The ANGUSTIFOLIA gene of Arabidopsis, a plant CtBP gene, regulates leaf-cell expansion, the arrangement of cortical microtubules in leaf cells and expression of a gene involved in cell-wall formation

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Gene expression

EXGT-A2 ... did not show such differences (not significantly different from the wild type; level of significance, 5%) when we compared an-1 and wild-type plants

Kim GT, Shoda K, Tsuge T, Cho KH, Uchimiya H, Yokoyama R, Nishitani K, Tsukaya H - The ANGUSTIFOLIA gene of Arabidopsis, a plant CtBP gene, regulates leaf-cell expansion, the arrangement of cortical microtubules in leaf cells and expression of a gene involved in cell-wall formation

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Gene expression

AN ... fusion construct with green fluorescent protein (GFP) under the control of the 35S promotor from the cauliflower mosaic virus ... AN±GFP fusion protein was found ... in the cytoplasm and the nucleus

Folkers U, Kirik V, Schöbinger U, Falk S, Krishnakumar S, Pollock MA, Oppenheimer DG, Day I, Reddy AS, Jürgens G, Hülskamp M - The cell morphogenesis gene ANGUSTIFOLIA encodes a CtBP/BARS-like protein and is involved in the control of the microtubule cytoskeleton

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Gene expression

we found AN expression in all tissues analyzed, including roots, leaves, stems and flowers

Folkers U, Kirik V, Schöbinger U, Falk S, Krishnakumar S, Pollock MA, Oppenheimer DG, Day I, Reddy AS, Jürgens G, Hülskamp M - The cell morphogenesis gene ANGUSTIFOLIA encodes a CtBP/BARS-like protein and is involved in the control of the microtubule cytoskeleton

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Gene expression

AN ... fusion construct with green fluorescent protein (GFP) under the control of the 35S promotor from the cauliflower mosaic virus ... AN±GFP fusion protein was found ubiquitously in the cytoplasm and the nucleus

Folkers U, Kirik V, Schöbinger U, Falk S, Krishnakumar S, Pollock MA, Oppenheimer DG, Day I, Reddy AS, Jürgens G, Hülskamp M - The cell morphogenesis gene ANGUSTIFOLIA encodes a CtBP/BARS-like protein and is involved in the control of the microtubule cytoskeleton

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Gene expression

weak expression of SDD1 mRNA was observed in the mesophyll cells of developing rosette leaves and leaf primordia and in all cell layers of the entire shoot apical meristem

Von Groll U, Berger D, Altmann T - The subtilisin-like serine protease SDD1 mediates cell-to-cell signaling during Arabidopsis stomatal development

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Gene expression

In the 35S-SDD1ct-GFP–overexpressing lines, green fluorescence corresponding to GFP was localized to the plasma membrane (Figures 5A to 5D) . Similar fluorescence patterns were observed when leaves were incubated for 1 to 12 h in a buffer at pH 7.0 in an attempt to neutralize the apoplast, because GFP is unstable at low pH (Haseloff et al., 1997) (Figures 5A and 5B). Plasmolysis with 1 M KNO3 confirmed the localization of the GFP fusion protein in association with the plasma membrane (Figure 5B). Because SDD1 has no predicted membrane-spanning domain, the 35S-SDD1ct-GFP fusion protein may be attached to the plasma membrane via additional factors

Von Groll U, Berger D, Altmann T - The subtilisin-like serine protease SDD1 mediates cell-to-cell signaling during Arabidopsis stomatal development

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Gene expression

In paradermal sections of the epidermis, these specialized cell types were identified as meristemoids/GMCs (Ms/GMCs) according to their characteristic shape, as described in Zhao and Sack (1999) (Figure 2D

Von Groll U, Berger D, Altmann T - The subtilisin-like serine protease SDD1 mediates cell-to-cell signaling during Arabidopsis stomatal development

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Gene expression

weak expression of SDD1 mRNA was observed in the mesophyll cells of developing rosette leaves and leaf primordia and in all cell layers of the entire shoot apical meristem

Von Groll U, Berger D, Altmann T - The subtilisin-like serine protease SDD1 mediates cell-to-cell signaling during Arabidopsis stomatal development

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Gene expression

weak expression of SDD1 mRNA was observed in the mesophyll cells of developing rosette leaves and leaf primordia and in all cell layers of the entire shoot apical meristem

Von Groll U, Berger D, Altmann T - The subtilisin-like serine protease SDD1 mediates cell-to-cell signaling during Arabidopsis stomatal development

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Gene expression

In paradermal sections of the epidermis, these specialized cell types were identified as meristemoids/GMCs (Ms/GMCs) according to their characteristic shape, as described in Zhao and Sack (1999) (Figure 2D

Von Groll U, Berger D, Altmann T - The subtilisin-like serine protease SDD1 mediates cell-to-cell signaling during Arabidopsis stomatal development

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Gene expression

no SDD1 mRNA was detected in mature GCs

Von Groll U, Berger D, Altmann T - The subtilisin-like serine protease SDD1 mediates cell-to-cell signaling during Arabidopsis stomatal development

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Gene expression

The results showed that SDD1 was expressed predominantly in specialized cell types in the epidermis of developing leaves and siliques

Von Groll U, Berger D, Altmann T - The subtilisin-like serine protease SDD1 mediates cell-to-cell signaling during Arabidopsis stomatal development

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Gene expression

in the Rbx1-depleted plants, we found a significant higher level of cyclin D3 protein (Fig. 9) whereas cyclin D3 mRNAs accumulated at a similar level than in the Dex non-induced or control plants (data not shown). However, this protein band is detected at ∼46 kDa, whereas cyclin D3 detected in roots is ∼55 kDa (47). Because the antibody was raised against the C-terminal peptide of cyclin D3 (47), the 46-kDa form of the protein must represent the loss of an N-terminal fragment or could be an alternative splice form or a modified form of the protein

Lechner E, Xie D, Grava S, Pigaglio E, Planchais S, Murray JA, Parmentier Y, Mutterer J, Dubreucq B, Shen WH, Genschik P - The AtRbx1 protein is part of plant SCF complexes, and its down-regulation causes severe growth and developmental defects

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Gene expression

RNA blot analyses were performed to investigate AtRbx1 expression, and its mRNAs could be detected in all the plant organs analyzed

Lechner E, Xie D, Grava S, Pigaglio E, Planchais S, Murray JA, Parmentier Y, Mutterer J, Dubreucq B, Shen WH, Genschik P - The AtRbx1 protein is part of plant SCF complexes, and its down-regulation causes severe growth and developmental defects

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Gene expression

To check whether the reductions of AtRbx1 and, to a lesser extent, AtCul1 would affect the pattern of ubiquitin conjugates, the blot was probed with an anti-ubiquitin antibody that recognizes both free and conjugated ubiquitin. Indeed, we noted that several protein bands either disappeared or were strongly reduced in the Dex-induced dsRNA line. The level of free ubiquitin also was reduced in those plants. However, the pattern of most ubiquitin conjugates were still similar between the non-induced and the induced line

Lechner E, Xie D, Grava S, Pigaglio E, Planchais S, Murray JA, Parmentier Y, Mutterer J, Dubreucq B, Shen WH, Genschik P - The AtRbx1 protein is part of plant SCF complexes, and its down-regulation causes severe growth and developmental defects

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Gene expression

To understand the biochemical basis of the severe growth defects observed in some of the dsRNA lines, protein extracts from dsRNA-2 line and control line were blotted with different antibodies (Fig. 9). Interestingly, we noted that AtRbx1-depleted seedlings do not only show a strong reduction of AtRbx1 protein level, but also a decreased AtCul1 protein level. In fact, both Rub1-modified and unmodified AtCul1 protein bands were weaker

Lechner E, Xie D, Grava S, Pigaglio E, Planchais S, Murray JA, Parmentier Y, Mutterer J, Dubreucq B, Shen WH, Genschik P - The AtRbx1 protein is part of plant SCF complexes, and its down-regulation causes severe growth and developmental defects

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Gene expression

diffuse localization of RHL2 throughout the nucleus

Sugimoto-Shirasu K, Stacey NJ, Corsar J, Roberts K, McCann MC - DNA topoisomerase VI is essential for endoreduplication in Arabidopsis

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Gene expression

BP was expressed in leaves of the ... yab3-2

Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis

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Gene expression

fil-8 yab3-2 plants, STM expression ... was restricted to the apical meristem

Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis

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Gene expression

expression of STM in the leaves ... not in wild-type plants

Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis

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Gene expression

In a wild-type background, BP::GUS expression is detected in the meristematic zone

Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis

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Gene expression

In seedlings heterozygous for yab3-2 ... we observed GUS expression on either side of the leaf margin in the younger tissues of leaf blades

Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis

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Gene expression

YAB3 ... In seedlings heterozygous for yab3-2, GUS expression was observed in the young leaves

Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis

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Gene expression

YAB3 ... In seedlings heterozygous for yab3-2 ... as the leaf matured, expression was restricted to the abaxial tissues of leaves

Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis

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Gene expression

fil-8 yab3-2 plants, STM expression was excluded from leaf anlagen

Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis

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Gene expression

KNAT2 ... enhanced level of expression was detected in the leaves of ... yab3-2

Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis

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Gene expression

In most of the fil-8 seedlings, BP::GUS was ... prominent in the leaf blade margins

Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis

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Gene expression

In wild-type plants, STM expression was excluded from leaf anlagen

Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis

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Gene expression

As lateral organs emerged from the apical meristem, the expression of BP::GUS was downregulated

Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis

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Gene expression

In some fil-8 seedlings, BP::GUS expression appeared to be restricted to the abaxial domains of the emerging leaves

Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis

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Gene expression

BP was expressed in leaves of the fil-8

Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis

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Gene expression

STM leaf expression was detectable even in fil-8

Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis

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Gene expression

STM leaf expression was detectable even in ... yab3-2 single-mutant plants

Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis

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Gene expression

We detected low-level expression of KNAT2 in the leaves of wild-type plants

Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis

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Gene expression

In most of the fil-8 seedlings, BP::GUS was detectable in emerging leaves

Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis

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Gene expression

stm mutant plants using the YAB3::GUS ... we detected no differences in the YAB gene expression pattern in the cotyledons of stm mutants and wild-type plants

Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis

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Gene expression

BP was expressed in leaves of ... fil-8 yab3-2 double mutants

Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis

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Gene expression

KNAT2 ... enhanced level of expression was detected in the leaves ... fil-8 yab3-2

Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis

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Gene expression

fil-8 yab3-2 plants ... We observed the expression of STM in the leaves of double mutants

Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis

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Gene expression

YAB3 expression also was more intense near the leaf margins (Figure 2B). Similar to the previous results with the fil-5 yab3-1 mutants (Siegfried et al., 1999

Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis

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Gene expression

We also tested, by RT-PCR, the levels of AS1 transcripts in fil yab3 leaves, and these appeared unchanged compared with those in wild-type leaves

Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis

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Gene expression

KNAT2 ... enhanced level of expression was detected in the leaves of ... fil-8

Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis

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Gene expression

In wild-type plants, STM expression was ... being restricted to the apical meristem

Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis

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Gene expression

BP was expressed in leaves ... not in wild-type plants

Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis

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Gene expression

SWP ... expression was maintained in developing leaves

Autran D, Jonak C, Belcram K, Beemster GT, Kronenberger J, Grandjean O, Inzé D, Traas J - Cell numbers and leaf development in Arabidopsis: a functional analysis of the STRUWWELPETER gene

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Gene expression

SWP ... A weak signal, sometimes just above the detection level, was also visible at the vegetative shoot apical meristem

Autran D, Jonak C, Belcram K, Beemster GT, Kronenberger J, Grandjean O, Inzé D, Traas J - Cell numbers and leaf development in Arabidopsis: a functional analysis of the STRUWWELPETER gene

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Gene expression

During embryogenesis, SWP mRNA was detected in all cells ... cotyledonar stage

Autran D, Jonak C, Belcram K, Beemster GT, Kronenberger J, Grandjean O, Inzé D, Traas J - Cell numbers and leaf development in Arabidopsis: a functional analysis of the STRUWWELPETER gene

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Gene expression

During embryogenesis, SWP mRNA was detected in all cells, from the early octant ... stage

Autran D, Jonak C, Belcram K, Beemster GT, Kronenberger J, Grandjean O, Inzé D, Traas J - Cell numbers and leaf development in Arabidopsis: a functional analysis of the STRUWWELPETER gene

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Gene expression

swp ... At the level of the young primordia, downregulation of STM ... was systematically observed

Autran D, Jonak C, Belcram K, Beemster GT, Kronenberger J, Grandjean O, Inzé D, Traas J - Cell numbers and leaf development in Arabidopsis: a functional analysis of the STRUWWELPETER gene

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Gene expression

in swp ... At the level of the young primordia ... upregulation of the primordium marker AINTEGUMENTA (ANT) was systematically observed

Autran D, Jonak C, Belcram K, Beemster GT, Kronenberger J, Grandjean O, Inzé D, Traas J - Cell numbers and leaf development in Arabidopsis: a functional analysis of the STRUWWELPETER gene

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Gene expression

SWP was strongly expressed in young leaf primordia

Autran D, Jonak C, Belcram K, Beemster GT, Kronenberger J, Grandjean O, Inzé D, Traas J - Cell numbers and leaf development in Arabidopsis: a functional analysis of the STRUWWELPETER gene

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Gene expression

KNAT6 ... expression ... in the cotyledons ... bop1-1 seedlings were significantly higher than those in wild- type seedlings

Ha CM, Kim GT, Kim BC, Jun JH, Soh MS, Ueno Y, Machida Y, Tsukaya H, Nam HG - The BLADE-ON-PETIOLE 1 gene controls leaf pattern formation through the modulation of meristematic activity in Arabidopsis

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Gene expression

in the petioles of cotyledons and rosette leaves ... KNAT1::GUS was misexpressed ... in the bop1- 1

Ha CM, Kim GT, Kim BC, Jun JH, Soh MS, Ueno Y, Machida Y, Tsukaya H, Nam HG - The BLADE-ON-PETIOLE 1 gene controls leaf pattern formation through the modulation of meristematic activity in Arabidopsis

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Gene expression

Examination of the expression pattern in single leaves of bop1-1 showed that, unlike in wild-type leaves, KNAT1::GUS was detected in the petioles of ... rosette leaves

Ha CM, Kim GT, Kim BC, Jun JH, Soh MS, Ueno Y, Machida Y, Tsukaya H, Nam HG - The BLADE-ON-PETIOLE 1 gene controls leaf pattern formation through the modulation of meristematic activity in Arabidopsis

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Gene expression

KNAT1 ... expression ... in the cotyledons ... of bop1-1 seedlings were significantly higher than those in wild- type seedlings

Ha CM, Kim GT, Kim BC, Jun JH, Soh MS, Ueno Y, Machida Y, Tsukaya H, Nam HG - The BLADE-ON-PETIOLE 1 gene controls leaf pattern formation through the modulation of meristematic activity in Arabidopsis

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Gene expression

In bop1-1 plants, KNAT1::GUS was expressed in a pattern similar to that in wild-type plants, but in more expanded regions

Ha CM, Kim GT, Kim BC, Jun JH, Soh MS, Ueno Y, Machida Y, Tsukaya H, Nam HG - The BLADE-ON-PETIOLE 1 gene controls leaf pattern formation through the modulation of meristematic activity in Arabidopsis

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Gene expression

bop1-1 ... STM transcription was not detectable in our experiment in the ... cotyledons

Ha CM, Kim GT, Kim BC, Jun JH, Soh MS, Ueno Y, Machida Y, Tsukaya H, Nam HG - The BLADE-ON-PETIOLE 1 gene controls leaf pattern formation through the modulation of meristematic activity in Arabidopsis

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Gene expression

KNAT2 ... bop1-1 ... there were no notable differences between the wild-type and mutant SAM regions in the expression

Ha CM, Kim GT, Kim BC, Jun JH, Soh MS, Ueno Y, Machida Y, Tsukaya H, Nam HG - The BLADE-ON-PETIOLE 1 gene controls leaf pattern formation through the modulation of meristematic activity in Arabidopsis

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Gene expression

KNAT1 ... bop1-1 ... there were no notable differences between the wild-type and mutant SAM regions in the expression

Ha CM, Kim GT, Kim BC, Jun JH, Soh MS, Ueno Y, Machida Y, Tsukaya H, Nam HG - The BLADE-ON-PETIOLE 1 gene controls leaf pattern formation through the modulation of meristematic activity in Arabidopsis

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Gene expression

Examination of the expression pattern in single leaves of bop1-1 showed that, unlike in wild-type leaves, KNAT1::GUS was detected in the petioles of cotyledons

Ha CM, Kim GT, Kim BC, Jun JH, Soh MS, Ueno Y, Machida Y, Tsukaya H, Nam HG - The BLADE-ON-PETIOLE 1 gene controls leaf pattern formation through the modulation of meristematic activity in Arabidopsis

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Gene expression

KNAT2 ... expression ... the cotyledons ... of bop1-1 seedlings were significantly higher than those in wild- type seedlings

Ha CM, Kim GT, Kim BC, Jun JH, Soh MS, Ueno Y, Machida Y, Tsukaya H, Nam HG - The BLADE-ON-PETIOLE 1 gene controls leaf pattern formation through the modulation of meristematic activity in Arabidopsis

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Gene expression

KNAT6 ... expression ... in the ... rosette leaves of bop1-1 seedlings were significantly higher than those in wild- type seedlings

Ha CM, Kim GT, Kim BC, Jun JH, Soh MS, Ueno Y, Machida Y, Tsukaya H, Nam HG - The BLADE-ON-PETIOLE 1 gene controls leaf pattern formation through the modulation of meristematic activity in Arabidopsis

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Gene expression

bop1-1 ... KNAT2 that showed only a slightly increased expression in the mutant rosette leaf

Ha CM, Kim GT, Kim BC, Jun JH, Soh MS, Ueno Y, Machida Y, Tsukaya H, Nam HG - The BLADE-ON-PETIOLE 1 gene controls leaf pattern formation through the modulation of meristematic activity in Arabidopsis

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Gene expression

KNAT1 ... expression ... in the ... rosette leaves of bop1-1 seedlings were significantly higher than those in wild- type seedlings

Ha CM, Kim GT, Kim BC, Jun JH, Soh MS, Ueno Y, Machida Y, Tsukaya H, Nam HG - The BLADE-ON-PETIOLE 1 gene controls leaf pattern formation through the modulation of meristematic activity in Arabidopsis

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Gene expression

bop1-1 ... STM transcription was not detectable in our experiment in the ... rosette leaves

Ha CM, Kim GT, Kim BC, Jun JH, Soh MS, Ueno Y, Machida Y, Tsukaya H, Nam HG - The BLADE-ON-PETIOLE 1 gene controls leaf pattern formation through the modulation of meristematic activity in Arabidopsis

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Gene expression

KNAT6 ... bop1-1 ... there were no notable differences between the wild-type and mutant SAM regions in the expression

Ha CM, Kim GT, Kim BC, Jun JH, Soh MS, Ueno Y, Machida Y, Tsukaya H, Nam HG - The BLADE-ON-PETIOLE 1 gene controls leaf pattern formation through the modulation of meristematic activity in Arabidopsis

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Gene expression

In wild-type plants, high levels of PNH mRNA are observed in developing vascular tissue

Newman KL, Fernandez AG, Barton MK - Regulation of axis determinacy by the Arabidopsis PINHEAD gene

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Gene expression

in situ hybridization experiments were performed on PNH; FIL::PNH transgenic plants using a PNH antisense probe. In addition to the wild-type expression domain, transformants expressed PNH ... near the margins on the abaxial sides of true leaves at levels resembling those on the adaxial sides

Newman KL, Fernandez AG, Barton MK - Regulation of axis determinacy by the Arabidopsis PINHEAD gene

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Gene expression

In wild-type plants ... PNH mRNA are observed ... on the adaxial, but not abaxial, sides of lateral organ primordia

Newman KL, Fernandez AG, Barton MK - Regulation of axis determinacy by the Arabidopsis PINHEAD gene

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Gene expression

in pnh; FIL::PNH embryos ... Similarly, STM was expressed only in its normal domain at the early and middle stages of embryogenesis, but it was expressed in some cotyledon primordia by the bent-cotyledon stage (Figures 8C and 8D). Unlike CUC2 expression, STM expression in cotyledon primordia was detected only after they were morphologically distinguishable as double cotyledons

Newman KL, Fernandez AG, Barton MK - Regulation of axis determinacy by the Arabidopsis PINHEAD gene

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Gene expression

Early in development, the expression of CUC2 in pnh; FIL::PNH embryos was confined to the same region as in the wild type (Figure 8A) . However, by the late heart stage, when cotyledon primordia are easily distinguishable, expression of this gene also was evident at the distal tip of some cotyledon primordia (Figure 8B). At later stages of embryogenesis, staining was seen in a higher proportion of cotyledon primordia

Newman KL, Fernandez AG, Barton MK - Regulation of axis determinacy by the Arabidopsis PINHEAD gene

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Gene expression

In wild-type plants ... PNH mRNA are observed in ... low levels ... throughout the SAM

Newman KL, Fernandez AG, Barton MK - Regulation of axis determinacy by the Arabidopsis PINHEAD gene

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Gene expression

in situ hybridization experiments were performed on PNH; FIL::PNH transgenic plants using a PNH antisense probe. In addition to the wild-type expression domain, transformants expressed PNH on the abaxial sides of cotyledons

Newman KL, Fernandez AG, Barton MK - Regulation of axis determinacy by the Arabidopsis PINHEAD gene

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Gene expression

no change in CYCD2;1 transcript levels was observed, despite the morphological and cytological changes apparent in CYCD3;1 OE plants

Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3

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Gene expression

Levels of Rb mRNA were increased in ... mature rosette leaves (by 3.5-fold ... in CYCD3;1 OE

Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3

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Gene expression

To determine whether the effects of CYCD3;1 on the G1/S transition also resulted in a modification of the spatial localization of cell division, we assessed the overall distribution of cell proliferation using a fusion of GUS to the promoter and destruction box of the mitotic cyclin CYCB1;1 (Colon-Carmona et al., 1999). The CYCB1-GUS fusion protein is present only in cells in G2/M and is destroyed rapidly when cells passed through mitosis, so GUS staining patterns indicate regions containing cells engaged in active cell division. CYCD3;1 OE seedlings carrying this construct stained for GUS over a wider region than wild-type control seedlings (Figure 4C

Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3

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Gene expression

large increase in Rb protein level also was detected in CYCD3;1 OE seedlings

Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3

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Gene expression

All E2F genes were upregulated in CYCD3;1 OE plants

Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3

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Gene expression

CYCD3;1 mRNA levels ... were not detected with a gel-based assay in mature rosette leaves

Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3

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Gene expression

We found that CYCD3;1 mRNA ... is present at high levels in young developing leaves

Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3

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Gene expression

CYCD3;1 OE ... SHOOTMERISTEMLESS ... mRNA levels ... were unchanged despite the dramatic morphological differences

Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3

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Gene expression

We found that CYCD3;1 mRNA is abundant ... particularly in peripheral meristem areas

Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3

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Gene expression

Little or no change in CYCD3;2 ... mRNA levels was observed in CYCD3;1 OE seedlings

Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3

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Gene expression

Low CYCD3;1 mRNA levels were detected in wild-type seedlings

Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3

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Gene expression

we found that mRNA levels of ANT were unaffected in the transgenic ... CYCD3;1 OE

Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3

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Gene expression

All E2F genes were upregulated in CYCD3;1 OE plants

Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3

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Gene expression

We found that CYCD3;1 mRNA is abundant in ... young primordia

Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3

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Gene expression

We found that CYCD3;1 mRNA ... In more mature developing leaves, a strong signal persists in adaxial cells

Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3

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Gene expression

We found that CYCD3;1 mRNA is abundant in the vegetative shoot meristem

Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3

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Gene expression

CYCD3;1 OE ... PHABULOSA ... mRNA levels ... were unchanged despite the dramatic morphological differences

Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3

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Gene expression

Little or no change in ... CYCD3;3 mRNA levels was observed in CYCD3;1 OE seedlings

Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3

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Gene expression

All E2F genes were upregulated in CYCD3;1 OE plants

Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3

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Gene expression

Levels of Rb mRNA were increased ... by 5.7-fold ... in CYCD3;1 OE seedlings

Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3

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Gene expression

pPHAB-GUS ... were introgressed into drl1-2 ... marker lines displayed promoter activity in the dorsal part of the leaf primordium, including the vascular bundles

Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants

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Gene expression

pDRL1-GUS ... transverse sections of 8- to 12-day-old SAMs ... section more apical to the SAM showed circular ... staining that coincided with the central zone

Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants

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Gene expression

pDRL1-GUS ... GUS activity also was observed typically around the vascular bun- dles

Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants

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Gene expression

pDRL1-GUS ... In young leaf primordia, GUS activity occurred as a linear area representing the basal part of the dorsal side

Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants

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Gene expression

pDRL1-GUS ... In older leaf primordia, X-Gluc staining was apparent as a continuous blue linear area ... the mesophyll be- tween the vascular bundles

Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants

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Gene expression

pDRL1-GUS ... In transverse serial sec- tions of expanding leaves, GUS activity was patchy: staining was seen in individual palisade and spongy mesophyll paren- chyma cells

Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants

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Gene expression

leaves ... mRNA of DRL1 was detected in every plant organ investigated in the wild type

Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants

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Gene expression

In the strong drl1-2 allele with severely reduced DRL1 transcript, a significant upregulation of the AN transcript (up to twofold) was seen

Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants

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Gene expression

pFIL-GUS ... introgressed into drl1-2 ... marker lines displayed promoter activity ... in the abaxial part of the leaf primordia, excluding vascular bundles

Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants

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Gene expression

pDRL1-GUS ... longitudinal sections through shoot and inflorescence apices, GUS activity was ... absent in the L3 layer

Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants

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Gene expression

pDRL1-GUS ... transverse sections of 8- to 12-day-old SAMs (n = 6), staining was ring shaped, indicating GUS activity in the periph- eral zone of the SAM

Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants

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Gene expression

pDRL1-GUS ... GUS activity was not observed in the mesophyll cells at the leaf tip

Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants

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Gene expression

pREV(DLUTA)-GUS ... were introgressed into drl1-2 ... marker lines displayed promoter activity in the dorsal part of the leaf primordium, including the vascular bundles

Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants

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Gene expression

pDRL1-GUS ... In longitudinal sections through shoot and inflorescence apices, GUS activity was most prominent in the L2 layer

Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants

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Gene expression

pDRL1-GUS ... GUS activity was not observed ... at the ventral mesophyll of the midrib

Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants

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Gene expression

The pDRL1-GUS pattern coincided with the patchy pat- tern of expression of pcyc1at-GUS during leaf development

Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants

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Gene expression

pDRL1-GUS ... Homogeneous GUS activity was detected in globular-, heart-, and torpedo-stage embryos

Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants

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Gene expression

pYAB3-GUS ... introgressed into drl1-2 ... marker lines displayed promoter activity ... in the abaxial part of the leaf primordia, excluding vascular bundles

Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants

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Gene expression

cotyledons ... mRNA of DRL1 was detected in every plant organ investigated in the wild type

Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants

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Gene expression

pDRL1-GUS ... In the leaf epidermis, GUS activity was restricted to the stomatal guard cells, which are generated by cell division from epidermal meristemoids after cell division has ceased in the epidermal pavement cells

Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants

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Gene expression

pDRL1-GUS ... GUS activity was not observed ... at the margin of the distal part of the leaf lamina

Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants

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Gene expression

pDRL1-GUS ... pattern was identi- cal to the pattern of the dorsal marker lines pPHAB-GUS and pREV-GUS in leaf primordia

Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants

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Gene expression

pDRL1-GUS ... In longitudinal sections through shoot and inflorescence apices, GUS activity was ... less prominent in the L1 layer

Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants

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Gene expression

shoot apices ... mRNA of DRL1 was detected in every plant organ investigated in the wild type

Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants

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Gene expression

pDRL1-GUS ... In older leaf primordia, X-Gluc staining was apparent as a continuous blue linear area, including vascular bundles

Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants

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Gene expression

We used RNA in situ hybridizations and a β-glucuronidase (GUS) reporter gene for expression analysis of DRN/ESR1. To construct the GUS reporter, the DRN/ESR1 coding region was replaced by the GUS open reading frame in a genomic clone carrying 4.8-kb DNA sequences upstream of the ATG and 1.5-kb DNA sequences downstream of the termination codon ... from the torpedo stage onward, transcripts were found exclusively in the L1 layer of the SAM

Kirch T, Simon R, Grünewald M, Werr W - The DORNROSCHEN/ENHANCER OF SHOOT REGENERATION1 gene of Arabidopsis acts in the control of meristem ccll fate and lateral organ development

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Gene expression

We used RNA in situ hybridizations and a β-glucuronidase (GUS) reporter gene for expression analysis of DRN/ESR1. To construct the GUS reporter, the DRN/ESR1 coding region was replaced by the GUS open reading frame in a genomic clone carrying 4.8-kb DNA sequences upstream of the ATG and 1.5-kb DNA sequences downstream of the termination codon ... When the seedling had germinated, expression was found in young leaf primordia

Kirch T, Simon R, Grünewald M, Werr W - The DORNROSCHEN/ENHANCER OF SHOOT REGENERATION1 gene of Arabidopsis acts in the control of meristem ccll fate and lateral organ development

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Gene expression

We used RNA in situ hybridizations and a β-glucuronidase (GUS) reporter gene for expression analysis of DRN/ESR1. To construct the GUS reporter, the DRN/ESR1 coding region was replaced by the GUS open reading frame in a genomic clone carrying 4.8-kb DNA sequences upstream of the ATG and 1.5-kb DNA sequences downstream of the termination codon ... expression was found ... in the two outer cell layers, L1 and L2, of the SAM

Kirch T, Simon R, Grünewald M, Werr W - The DORNROSCHEN/ENHANCER OF SHOOT REGENERATION1 gene of Arabidopsis acts in the control of meristem ccll fate and lateral organ development

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Gene expression

We used RNA in situ hybridizations and a β-glucuronidase (GUS) reporter gene for expression analysis of DRN/ESR1. To construct the GUS reporter, the DRN/ESR1 coding region was replaced by the GUS open reading frame in a genomic clone carrying 4.8-kb DNA sequences upstream of the ATG and 1.5-kb DNA sequences downstream of the termination codon ... At the early heart stage, transcripts accumulated at high levels in the emerging cotyledons and at lower levels at the position of the prospective SAM (Figure 3B). Expression in cotyledons was only transient

Kirch T, Simon R, Grünewald M, Werr W - The DORNROSCHEN/ENHANCER OF SHOOT REGENERATION1 gene of Arabidopsis acts in the control of meristem ccll fate and lateral organ development

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Gene expression

We used RNA in situ hybridizations and a β-glucuronidase (GUS) reporter gene for expression analysis of DRN/ESR1. To construct the GUS reporter, the DRN/ESR1 coding region was replaced by the GUS open reading frame in a genomic clone carrying 4.8-kb DNA sequences upstream of the ATG and 1.5-kb DNA sequences downstream of the termination codon ... expression was detected first in the proembryo at the four-cell stage (Figure 3A) and throughout the embryo at the globular stage

Kirch T, Simon R, Grünewald M, Werr W - The DORNROSCHEN/ENHANCER OF SHOOT REGENERATION1 gene of Arabidopsis acts in the control of meristem ccll fate and lateral organ development

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Gene expression

we produced transgenic Arabidopsis plants expressing a HST-GUS fusion protein under the regulation of the CaMV 35S promoter. hst-1 ... In trichomes ... GUS staining was clearly localized in the vicinity of the nuclear membrane

Bollman KM, Aukerman MJ, Park MY, Hunter C, Berardini TZ, Poethig RS - HASTY, the Arabidopsis ortholog of exportin 5/MSN5, regulates phase change and morphogenesis

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Gene expression

we produced transgenic Arabidopsis plants expressing a HST-GUS fusion protein under the regulation of the CaMV 35S promoter. hst-1 plants containing the transgene were phenotypically wild type ... GUS activity was concentrated in the nucleus ... in ... trichomes

Bollman KM, Aukerman MJ, Park MY, Hunter C, Berardini TZ, Poethig RS - HASTY, the Arabidopsis ortholog of exportin 5/MSN5, regulates phase change and morphogenesis

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Gene expression

we produced transgenic Arabidopsis plants expressing a HST-GUS fusion protein under the regulation of the CaMV 35S promoter. hst-1 ... In trichomes ... GUS staining ... was always most intense on the apical side of the nucleus

Bollman KM, Aukerman MJ, Park MY, Hunter C, Berardini TZ, Poethig RS - HASTY, the Arabidopsis ortholog of exportin 5/MSN5, regulates phase change and morphogenesis

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Gene expression

HST ... Hybridization of this cDNA to poly(A)-enriched RNA revealed a single 4 kb transcript in leaves

Bollman KM, Aukerman MJ, Park MY, Hunter C, Berardini TZ, Poethig RS - HASTY, the Arabidopsis ortholog of exportin 5/MSN5, regulates phase change and morphogenesis

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Gene expression

we produced transgenic Arabidopsis plants expressing a HST-GUS fusion protein under the regulation of the CaMV 35S promoter. hst-1 plants containing the transgene were phenotypically wild type ... GUS activity was concentrated in the nucleus and the surrounding cytoplasm ... in ... trichomes

Bollman KM, Aukerman MJ, Park MY, Hunter C, Berardini TZ, Poethig RS - HASTY, the Arabidopsis ortholog of exportin 5/MSN5, regulates phase change and morphogenesis

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Gene expression

very young primordia of the shoot apical meristem ... expression was considerable ... of ... IGS

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

DR5::GUS ... In the young plants examined ... cotyledons did not show any GUS expression

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

DR5::GUS ... Different patterns of reporter-gene expression were observed in the well-developed 9-week-old plants, characterized by late-formed enlarged leaves (typical of short-day conditions), which continuously showed distinct patterns with GUS activity in the lobes

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

In later developmental stages, in 5- to 9-mm-long leaves of 9-week-old plants, the expression of ... ILL was again similar in the margin and in the lamina

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

A striking difference between the shoot and the root was found in the extent of DR5::GUS expression in their apices. In none of the 100 shoot tips studied could GUS activity be detected in the ... youngest leaves

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

DR5::GUS ... In advanced stages of primordium development ... GUS activity occurred (away from the leaf margin) at the tips of the freely ending veinlets, or at intermediate sites along these smallest blind veinlets

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

DR5::GUS ... In the young plants examined, the two lowest leaves were almost free of GUS activity

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

DR5::GUS ... Different patterns of reporter-gene expression were observed in the well-developed 9-week-old plants, characterized by late-formed enlarged leaves (typical of short-day conditions), which continuously showed distinct patterns with GUS activity ... but nearly no expression in the lamina

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

DR5::GUS ... To ensure that this pattern of GUS expression did not reflect inhibition of penetration or reaction of -glucuronide, incisions were made in 24 leaf primordia at various developmental stages. By exposing the mesophyll to the GUS-staining solution, no induction of GUS expression could be detected along the inner-leaf surfaces (Fig. 1e). This incision control confirmed that no GUS activity existed below the free-auxin-producing tip during this early stage of leaf growth

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

DR5::GUS ... in the upper rosette leaves with slightly serrate edges, the marginal GUS expression was mainly restricted to the lobes

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

DR5::GUS ... At higher microscopic magnification, GUS expression was also observed in some of the bundles (Fig. 2c), indicating auxin flow within these veins

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

DR5::GUS ... In more developed primordia, new sites of GUS activity appeared basipetally along the distal regions of the leaf margin

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

In later developmental stages, in 5- to 9-mm-long leaves of 9-week-old plants, the expression of IGS ... was again similar in the margin and in the lamina

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

in very young primordia of the shoot apical meristem ... CHS expression was considerable

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

DR5::GUS ... Large hydathodes develop in the leaf tip and in the lobes. During the early stages of their differentiation, a center of high free-auxin production could be detected as a concentrated dark-blue GUS stain in the lobe (Fig. 2b, see Fig. 7b-d). From this very active center, a downward gradual decrease in GUS activity was observed from the margin to the differentiating bundle, which is assumed to function as a sink for the auxin flow originating in the primordium tip or in a lobe (Fig. 2b). In more developed hydathodes, the differentiating vessel elements extended towards the lobe margin, usually with more vessel elements in the distal parts of the bundle near the margin (Fig. 2c). Elevated GUS expression persisted in the lobes after xylem maturation in the hydathode (Fig. 3a, b

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

Interestingly, chalcone synthase, the key enzyme for biosynthesis of flavonoids, which are natural inhibitors of basipetal IAA transport and of IAA-degrading peroxidase, was strongly and preferentially expressed in the margin

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

In later developmental stages, in 5- to 9-mm-long leaves of 9-week-old plants, the expression of ... ILL was again similar in the margin and in the lamina

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

In later developmental stages, in 5- to 9-mm-long leaves of 9-week-old plants, the expression of IGS ... was again similar in the margin and in the lamina

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

The largest difference was found in the expression of PIN1, an essential component of the basipetally directed auxin carrier (G�lweiler et al. 1998), with strong expression in the hydathode-containing margin

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

DR5::GUS ... Gradients of decreasing GUS expression could be detected along the margins of early developing primordia, being stronger in the distal than in the proximal parts below

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

CHS expression was more pronounced now in the margin

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

indole-3-glycerol phosphate synthase (IGS), regarded as a key enzyme of the tryptophan-independent IAA synthesis pathway (Normanly and Bartel 1999), was strongly expressed along the hydathode-containing margin

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

DR5::GUS ... Different patterns of reporter-gene expression were observed in the well-developed 9-week-old plants, characterized by late-formed enlarged leaves (typical of short-day conditions), which continuously showed distinct patterns with GUS activity in the ... central part of the midvein

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

DR5::GUS ... Elevated GUS activity also occurred at and above bundle junctions along the primary and secondary veins (1*) as well as along the tertiary veins

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

IAA-amino acid hydrolase (ILL), which catalyzes the hydrolysis of IAA-amino acid conjugates (Normanly and Bartel 1999), was well expressed in ... lamina

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

DR5::GUS ... In advanced stages of primordium development, GUS expression was detected in the mesophyll, in central regions of the lamina

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

The expression of NIT in very young primordia of the shoot apical meristem (Fig. 4a) was clearly stronger than in leaflets of 9-week-old plants

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

The largest difference was found in the expression of PIN1, an essential component of the basipetally directed auxin carrier ... which was nearly absent in the lamina

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

DR5::GUS ... In young leaf primordia, i.e., in the second or third leaf (counted from the shoot apex), relatively low GUS activity was detected in the tip

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

CHS expression was ... nearly absent in the lamina

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

DR5::GUS ... GUS activity was visible at the base of some of the trichomes (Fig. 2a, g). During leaf primordium development, the trichomes showing GUS expression appeared in different sites along the primordium, near to, or far away from the margins. Those developing along the margins showed stronger GUS activity than those in central regions of the lamina

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

DR5::GUS ... In advanced stages of primordium development, GUS expression was detected ... at the sites of small vein formation (usually tertiary veins)

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

Nitrilase (NIT 1-3), a key enzyme in the tryptophan-dependent IAA synthesis pathway (Hillebrand et al. 1998; Normanly and Bartel 1999), was equally expressed in ... lamina

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

In young leaf primordia 2-5 mm long (compare Fig. 1g, i), indole-3-glycerol phosphate synthase (IGS), regarded as a key enzyme of the tryptophan-independent IAA synthesis pathway (Normanly and Bartel 1999), was ... expressed ... strongly in the lamina

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

IAA-amino acid hydrolase (ILL), which catalyzes the hydrolysis of IAA-amino acid conjugates (Normanly and Bartel 1999), was well expressed in ... margin

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

DR5::GUS ... Different patterns of reporter-gene expression were observed in the well-developed 9-week-old plants, characterized by late-formed enlarged leaves (typical of short-day conditions), which continuously showed distinct patterns with GUS activity in the ... margin

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

DR5::GUS ... In the lowest rosette leaves, characterized by rounded blades, GUS activity had spread evenly downward from the tip

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

DR5::GUS ... Later on, the GUS activity boosted and appeared as a dark blue point in the tip (Fig. 1f, see Fig. 7b). At this stage, no GUS expression could be detected in the leaf tissue below the free-auxin-producing tip of the young primordium, either along the margins, or in the lamina

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

Interestingly, chalcone synthase, the key enzyme for biosynthesis of flavonoids, which are natural inhibitors of basipetal IAA transport and of IAA-degrading peroxidase ... was ... weakly expressed in the lamina of the primordia

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

In the youngest shoot region, a typical strong DR5::GUS expression was first detected in the stipules

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

in very young primordia of the shoot apical meristem ... expression was considerable ... of ... PIN

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

A striking difference between the shoot and the root was found in the extent of DR5::GUS expression in their apices. In none of the 100 shoot tips studied could GUS activity be detected in the shoot promeristem

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

in very young primordia of the shoot apical meristem ... expression was considerable ... of ILL

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

Nitrilase (NIT 1-3), a key enzyme in the tryptophan-dependent IAA synthesis pathway (Hillebrand et al. 1998; Normanly and Bartel 1999), was equally expressed in ... margin

Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis

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Gene expression

in-frame fusion between RCE1 and the β-glucuronidase (GUS) gene ... In leaves, particularly strong staining was observed in the ... trichomes

Dharmasiri S, Dharmasiri N, Hellmann H, Estelle M - The RUB/Nedd8 conjugation pathway is required for early development in Arabidopsis

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Gene expression

we examined the status of CUL1 in wild-type and rce1-1 plants by immunoblotting. Figure 3A shows that the level of RUB–CUL1 in the mutant is significantly reduced compared with Ler plants

Dharmasiri S, Dharmasiri N, Hellmann H, Estelle M - The RUB/Nedd8 conjugation pathway is required for early development in Arabidopsis

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Gene expression

in-frame fusion between RCE1 and the β-glucuronidase (GUS) gene ... intense staining was observed at the shoot apex

Dharmasiri S, Dharmasiri N, Hellmann H, Estelle M - The RUB/Nedd8 conjugation pathway is required for early development in Arabidopsis

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Gene expression

in-frame fusion between RCE1 and the β-glucuronidase (GUS) gene ... In leaves, particularly strong staining was observed in the veins

Dharmasiri S, Dharmasiri N, Hellmann H, Estelle M - The RUB/Nedd8 conjugation pathway is required for early development in Arabidopsis

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Gene expression

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Dharmasiri S, Dharmasiri N, Hellmann H, Estelle M - The RUB/Nedd8 conjugation pathway is required for early development in Arabidopsis

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Gene expression

To investigate the pattern of RCE1 expression, we first examined expression in various plant tissues by RNA blotting. As indicated in Figure 1A, RCE1 RNA accumulates to substantial levels in all plant organs examined

Dharmasiri S, Dharmasiri N, Hellmann H, Estelle M - The RUB/Nedd8 conjugation pathway is required for early development in Arabidopsis

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Gene expression

jaw-D ... TCP gene with strong similarity to CIN was also downregulated

Palatnik JF, Allen E, Wu X, Schommer C, Schwab R, Carrington JC, Weigel D - Control of leaf morphogenesis by microRNAs

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Gene expression

jaw-D ... TCP gene with strong similarity to CIN was also downregulated

Palatnik JF, Allen E, Wu X, Schommer C, Schwab R, Carrington JC, Weigel D - Control of leaf morphogenesis by microRNAs

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Gene expression

During the torpedo stage, TCP4 RNA becomes localized to subepidermal regions of the cotyledons, with highest levels near the tip (e–g). h, A similar pattern as in cotyledons is seen in young leaves of a wild-type seedling

Palatnik JF, Allen E, Wu X, Schommer C, Schwab R, Carrington JC, Weigel D - Control of leaf morphogenesis by microRNAs

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Gene expression

TCP4 RNA becomes gradually restricted to emerging cotyledons during the heart stage

Palatnik JF, Allen E, Wu X, Schommer C, Schwab R, Carrington JC, Weigel D - Control of leaf morphogenesis by microRNAs

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Gene expression

TCP4 mRNA in wild-type embryos ... initially uniform pattern during the globular stage

Palatnik JF, Allen E, Wu X, Schommer C, Schwab R, Carrington JC, Weigel D - Control of leaf morphogenesis by microRNAs

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Gene expression

jaw-D ... TCP gene with strong similarity to CIN was also downregulated

Palatnik JF, Allen E, Wu X, Schommer C, Schwab R, Carrington JC, Weigel D - Control of leaf morphogenesis by microRNAs

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Gene expression

Using RT–qPCR, we found that the MIR-JAW precursor was more abundant in the shoot apex, inflorescence and siliques

Palatnik JF, Allen E, Wu X, Schommer C, Schwab R, Carrington JC, Weigel D - Control of leaf morphogenesis by microRNAs

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Gene expression

jaw-D ... TCP gene with strong similarity to CIN was also downregulated

Palatnik JF, Allen E, Wu X, Schommer C, Schwab R, Carrington JC, Weigel D - Control of leaf morphogenesis by microRNAs

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Gene expression

jaw-D ... TCP gene with strong similarity to CIN was also downregulated

Palatnik JF, Allen E, Wu X, Schommer C, Schwab R, Carrington JC, Weigel D - Control of leaf morphogenesis by microRNAs

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Gene expression

Using RT–qPCR, we found that the MIR-JAW precursor was ... elevated in jaw-D mutants (Fig. 3b), mimicking the differential accumulation of the processed miRNA in different tissues and in jaw-D

Palatnik JF, Allen E, Wu X, Schommer C, Schwab R, Carrington JC, Weigel D - Control of leaf morphogenesis by microRNAs

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Gene expression

During the torpedo stage, TCP4 RNA becomes localized to subepidermal regions of the cotyledons, with highest levels near the tip

Palatnik JF, Allen E, Wu X, Schommer C, Schwab R, Carrington JC, Weigel D - Control of leaf morphogenesis by microRNAs

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Gene expression

plants expressing an ARGOS– β-glucuronidase(GUS) ... GUS expression was detected in cotyledon vascular bundles

Hu Y, Xie Q, Chua NH - The Arabidopsis auxin-inducible gene ARGOS controls lateral organ size

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Gene expression

ARGOS induction by NAA still was observed in ... aux1-7

Hu Y, Xie Q, Chua NH - The Arabidopsis auxin-inducible gene ARGOS controls lateral organ size

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Gene expression

ARGOS was expressed at low levels in ... young rosette leaves

Hu Y, Xie Q, Chua NH - The Arabidopsis auxin-inducible gene ARGOS controls lateral organ size

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Gene expression

ARGOS induction by NAA still was observed in axr2

Hu Y, Xie Q, Chua NH - The Arabidopsis auxin-inducible gene ARGOS controls lateral organ size

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Gene expression

de- creased levels of ARGOS expression were observed in ... axr1-12 ... and the induction of ARGOS by NAA was ... abolished completely in axr1- 12

Hu Y, Xie Q, Chua NH - The Arabidopsis auxin-inducible gene ARGOS controls lateral organ size

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Gene expression

ARGOS induction by NAA still was observed in ... tir1-3

Hu Y, Xie Q, Chua NH - The Arabidopsis auxin-inducible gene ARGOS controls lateral organ size

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Gene expression

plants expressing an ARGOS– β-glucuronidase(GUS) ... 12-day-old seedlings, GUS staining was observed ... juvenile leaf blades

Hu Y, Xie Q, Chua NH - The Arabidopsis auxin-inducible gene ARGOS controls lateral organ size

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Gene expression

we investigated the cellular localization of ARGOS in onion epi- dermal cells using 35S-ARGOS-GFP and 35S-GFP-ARGOS fusion genes ... ARGOS is distributed in the nucleus

Hu Y, Xie Q, Chua NH - The Arabidopsis auxin-inducible gene ARGOS controls lateral organ size

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Gene expression

ARGOS induction by NAA still was observed in ... iaa28

Hu Y, Xie Q, Chua NH - The Arabidopsis auxin-inducible gene ARGOS controls lateral organ size

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Gene expression

we investigated the cellular localization of ARGOS in onion epi- dermal cells using 35S-ARGOS-GFP and 35S-GFP-ARGOS fusion genes ... ARGOS is distributed in the ... cytosol

Hu Y, Xie Q, Chua NH - The Arabidopsis auxin-inducible gene ARGOS controls lateral organ size

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Gene expression

de- creased levels of ARGOS expression were observed in axr1-3 ... and the induction of ARGOS by NAA was attenu- ated in the weak allele axr1-3

Hu Y, Xie Q, Chua NH - The Arabidopsis auxin-inducible gene ARGOS controls lateral organ size

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Gene expression

plants expressing an ARGOS– β-glucuronidase(GUS) ... In 12-day-old seedlings, GUS staining was observed ... juvenile leaf ... petioles

Hu Y, Xie Q, Chua NH - The Arabidopsis auxin-inducible gene ARGOS controls lateral organ size

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Gene expression

ANT expression persisted in rosette leaves of 40-day-old 35S-ARGOS plants

Hu Y, Xie Q, Chua NH - The Arabidopsis auxin-inducible gene ARGOS controls lateral organ size

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Gene expression

plants expressing an ARGOS– β-glucuronidase(GUS) ... In 12-day-old seedlings, GUS staining was observed clearly in leaf primordia

Hu Y, Xie Q, Chua NH - The Arabidopsis auxin-inducible gene ARGOS controls lateral organ size

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Gene expression

ARGOS ... was almost undetectable in mature leaves

Hu Y, Xie Q, Chua NH - The Arabidopsis auxin-inducible gene ARGOS controls lateral organ size

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Gene expression

prolonged expression of CycD3;1 also was detected in 35S-ARGOS leaves

Hu Y, Xie Q, Chua NH - The Arabidopsis auxin-inducible gene ARGOS controls lateral organ size

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Gene expression

One gene, which is identical to the putative gene At3g59900 in the Arabidopsis database, was found to be highly induced by NAA (data not shown). We designated this gene ARGOS (for Auxin-Regulated Gene involved in Organ Size; see below). RNA gel blot analysis confirmed the auxin induction of ARGOS in both roots and aerial parts (Figure 1B

Hu Y, Xie Q, Chua NH - The Arabidopsis auxin-inducible gene ARGOS controls lateral organ size

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Gene expression

AtGRF7 ... predominantly expressed in shoot tips and flowers

Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis

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Gene expression

AtGRF1 through AtGRF6 ... expression was low in mature stems and leaves

Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis

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Gene expression

Leaf tissues were stained ... over the whole leaf area in ... AtGRF2::GUS

Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis

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Gene expression

AtGRF1 through AtGRF6 genes were strongly expressed in roots, upper stems, and shoot tips containing the shoot apical meristem (SAM) and flower buds, as well as in mature flowers

Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis

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Gene expression

The SAM tissues were also stained in seedlings and at the reproductive stage of AtGRF3::GUS plants

Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis

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Gene expression

AtGRF1 through AtGRF6 ... expression was low in mature stems and leaves

Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis

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Gene expression

AtGRF1 through AtGRF6 genes were strongly expressed in roots, upper stems, and shoot tips containing the shoot apical meristem (SAM) and flower buds, as well as in mature flowers

Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis

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Gene expression

AtGRF1 through AtGRF6 genes were strongly expressed in roots, upper stems, and shoot tips containing the shoot apical meristem (SAM) and flower buds, as well as in mature flowers

Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis

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Gene expression

AtGRF1 through AtGRF6 genes were strongly expressed in roots, upper stems, and shoot tips containing the shoot apical meristem (SAM) and flower buds, as well as in mature flowers

Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis

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Gene expression

AtGRF1 through AtGRF6 ... expression was low in mature stems and leaves

Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis

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Gene expression

AtGRF1 through AtGRF6 ... expression was low in mature stems and leaves

Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis

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Gene expression

AtGRF1 through AtGRF6 genes were strongly expressed in roots, upper stems, and shoot tips containing the shoot apical meristem (SAM) and flower buds, as well as in mature flowers

Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis

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Gene expression

The transcript of AtGRF9 was detected in the SAM and flower buds only after a prolonged exposure of the PhosphoImager screen (data not shown

Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis

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Gene expression

Staining in leaves of AtGRF3::GUS plants was restricted to the very early stage of emerging leaves (Figure 3l), after which no staining in leaves was visible anymore (data not shown), reflecting the low transcript level of AtGRF3 in leaf tissues on an RNA gel blot

Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis

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Gene expression

AtGRF1 through AtGRF6 genes were strongly expressed in roots, upper stems, and shoot tips containing the shoot apical meristem (SAM) and flower buds, as well as in mature flowers

Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis

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Gene expression

AtGRF1 through AtGRF6 ... expression was low in mature stems and leaves

Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis

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Gene expression

AtGRF8 ... predominantly expressed in shoot tips and flowers

Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis

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Gene expression

Leaf tissues were stained along the veins ... in AtGRF1::GUS

Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis

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Gene expression

AtGRF1 through AtGRF6 ... expression was low in mature stems and leaves

Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis

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Gene expression

as1 35S:AS2 ... We detected no change in BP transcript levels in these plants

Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning

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Gene expression

Transcript levels of ... YAB3 ... were reduced in 35S:AS2 shoots

Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning

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Gene expression

AS2 transcript levels were increased slightly in ... fil-8 yab3-2 double mutants

Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning

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Gene expression

PHB in as2-1 mutants. No significant differences in the transcript levels

Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning

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Gene expression

AS2 transcript levels were increased slightly in ... phb-1d mutants

Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning

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Gene expression

35S:AS2 ... Higher levels of WUS transcripts also were detected in class-IIB seedlings

Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning

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Gene expression

35S:AS2 ... reduction in ... KNAT6 transcript levels was observed in ... transgenic seedlings

Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning

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Gene expression

In kan1 kan2 double mutants, AS2 transcripts were de- tected at wild-type levels

Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning

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Gene expression

FIL ... in as2-1 mutants. No significant differences in the transcript levels

Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning

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Gene expression

AS1 transcripts were detected in both petioles and blades of rosette leaves and appeared to be slightly more abundant in blade tissue, whereas AS2 transcripts were more abun- dant in petioles

Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning

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Gene expression

35S:AS2 ... reduction in KNAT2 ... transcript levels was observed in ... transgenic seedlings

Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning

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Gene expression

KAN2 ... in as2-1 mutants. No significant differences in the transcript levels

Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning

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Gene expression

KAN2 transcripts were detectable in 35S:AS2 shoots, but at reduced levels compared with those in the wild type

Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning

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Gene expression

KAN1 ... in as2-1 mutants. No significant differences in the transcript levels

Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning

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Gene expression

AS1 transcripts were detected in all tissues tested

Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning

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Gene expression

YAB3 ... in as2-1 mutants. No significant differences in the transcript levels

Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning

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Gene expression

LOB transcripts were not detected in rosette leaf blades

Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning

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Gene expression

Transcript levels of FIL ... were reduced in 35S:AS2 shoots

Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning

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Gene expression

35S:AS2 ... reduction in ... BP transcript levels

Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning

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Gene expression

KAN1 ... transcripts were detectable in 35S:AS2 shoots, but at reduced levels compared with those in the wild type

Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning

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Gene expression

35S:AS2 plants ... KNAT2:GUS expression ... was reduced in some class- I seedlings and all class-II individuals

Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning

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Gene expression

LOB transcripts ... were detected in petioles

Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning

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Gene expression

35S:AS2 ... STM transcript levels ... were increased in class-IIB seedlings

Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning

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Gene expression

AS1 transcripts were detected in both petioles and blades of rosette leaves and appeared to be slightly more abundant in blade tissue, whereas AS2 transcripts were more abun- dant in petioles

Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning

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Gene expression

the majority of class-I 35S:AS2 plants retained high lev- els of KNAT2:GUS expression, similar to the parental line

Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning

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Gene expression

AS2 transcripts ... were detected in cotyledons

Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning

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Gene expression

AS2 transcripts were detected in rosette leaves, where they were more abundant in the petiole than in the blade

Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning

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Gene expression

BP:GUS background, all 102 seedlings carrying the 35S:AS2 transgene showed a reduction in staining intensity relative to the wild type

Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning

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Gene expression

transcript levels of PHB, a gene that regulates adaxial identity, were increased in 35S:AS2 shoots of both classes, with the more severe class-IIB seedlings showing higher levels than class-I seedlings

Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning

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Gene expression

AS2 transcripts were detected in most tissues tested

Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning

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Gene expression

At 2 DAG, ARR5:GUS activity was completely absent in the shoot apical meristem (SAM) of 35S:AtCKX1 transgenic plants

Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity

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Gene expression

AtCKX5:GUS ... was detected in the rib zone of the axillary shoot meristems

Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity

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Gene expression

AtCKX4:GUS ... GUS staining was present in epidermal pavement cells

Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity

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Gene expression

AtCKX6:GUS ... the AtCKX6 promoter was active during the maturation phase of stomatal guard cells but not in fully mature stomata in older leaves

Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity

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Gene expression

epidermal leaf cells of plants expressing high levels of AtCKX3-GFP accu- mulated fluorescence in the large central vacuole

Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity

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Gene expression

AtCKX6:GUS expression ... was undetectable in fully expanded leaves

Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity

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Gene expression

AtCKX1-GFP ... was not detected in vacuoles. However, AtCKX1-GFP fluorescence was detected in vacuoles of mainly smaller root cells, such as those of the vascular cylinder (Figure 10K). This finding suggests that vacuolar targeting of AtCKX1-GFP might be specific to certain cell types, or, more likely, that the AtCKX1-GFP expression level was lower and thus stained only smaller vacuoles. To- gether, these data support the conclusion that the final destina- tion of AtCKX1 and AtCKX3 is the vacuole

Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity

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Gene expression

ARR5:GUS activity was reduced strongly during various developmental stages of 35S:AtCKX1 plants

Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity

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Gene expression

Very strong AtCKX4:GUS expression was detected in stipules

Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity

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Gene expression

In the shoot, AtCKX5:GUS expression was confined to the edges at the most basal part of the youngest leaves

Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity

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Gene expression

AtCKX6:GUS expression was localized primarily to the vas- cular system of developing cotyledons

Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity

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Gene expression

AtCKX2-GFP fluorescence to be distributed as a thin, more or less discontinuous line at the cell periphery and around nuclei (Figure 10D). In an optical section at the level of the cor- tex cytoplasm, the fluorescence appeared as a reticulate po- lygonal network often linked with punctuated structures (Fig- ure 10E). Based on published data, this distinct pattern can be identified as indicating localization to the ER

Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity

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Gene expression

AtCKX2 promoter was detected in the shoot apex

Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity

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Gene expression

High AtCKX4:GUS activity was localized to trichomes

Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity

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Gene expression

AtCKX6:GUS expression was localized primarily to the vas- cular system of developing ... leaves

Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity

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Gene expression

AtCKX3: GUS transformants, in which GUS expression was driven by a 1.46-kb promoter fragment, we occasionally detected very weak expression in the young shoot tissues 2 weeks after germination (data not shown

Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity

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Gene expression

AtCKX1 promoter drove gene expression in the ... lateral shoot meristems

Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity

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Gene expression

AtCKX6:GUS ... activity was strongest in the vasculature of young growing leaves. It gradually decreased basipetally in older leaves and was undetectable in fully expanded leaves

Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity

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Gene expression

AtCKX2 promoter was detected ... in stipules

Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity

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Gene expression

AtCKX3-GFP entered the ER lumen

Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity

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Gene expression

AtCKX1 promoter drove gene expression in the shoot apex

Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity

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Gene expression

the shoot, AtCKX5:GUS expression ... marking the developing leaf petiole

Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity

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Gene expression

AtCKX1-GFP fusion marked the ER network in leaf epidermal cells

Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity

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Gene expression

In Northern blot experiments, ATHB16 mRNA was detected in all organs examined, the mRNA level being relatively high in rosette leaves, intermediate in roots, cauline leaves, inflorescences, and buds, but low in the stem and in siliques, as shown in Fig. 2A

Wang Y, Henriksson E, Söderman E, Henriksson KN, Sundberg E, Engström P - The Arabidopsis homeobox gene, ATHB16, regulates leaf development and the sensitivity to photoperiod in Arabidopsis

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Gene expression

DHS transcript was detectable in RNA preparations from leaves at all stages, which indicates that expression of this gene is required throughout the course of leaf development and senescence (Figure 1B). Of particular interest, however, is the finding that DHS expression was strongly upregulated in the third pair of rosette leaves between days 7 and 14 after emergence (Figure 1B). This upregulation coincides with bolting, which marks initiation of the conversion from vegetative to reproductive growth. Between days 14 and 21 after emergence, DHS transcript levels in the third pair of rosette leaves declined again (Figure 1B). However, there was a further increase in transcript abundance through days 28 and 35 after emergence during the period when there is extensive catabolism of macromolecules within the dying cells and concurrent translocation of nutrients out of the senescing leaves into the developing seeds

Wang TW, Lu L, Zhang CG, Taylor C, Thompson JE - Pleiotropic effects of suppressing deoxyhypusine synthase expression in Arabidopsis thaliana

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Gene expression

We found no significant differences in the expression of the ... CycD3 ... the wild-type plants after auxin ... treatment

Pérez-Pérez JM, Ponce MR, Micol JL - The ULTRACURVATA2 gene of Arabidopsis encodes an FK506-binding protein involved in auxin and brassinosteroid signaling

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Gene expression

We found no significant differences in the expression of the ... CycD3 ... in the wild-type plants after ... BR treatment

Pérez-Pérez JM, Ponce MR, Micol JL - The ULTRACURVATA2 gene of Arabidopsis encodes an FK506-binding protein involved in auxin and brassinosteroid signaling

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Gene expression

CPD ... down-regulated by brassinolide in the wild types studied, an effect that is abolished or extremely reduced by the ucu2 mutations

Pérez-Pérez JM, Ponce MR, Micol JL - The ULTRACURVATA2 gene of Arabidopsis encodes an FK506-binding protein involved in auxin and brassinosteroid signaling

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Gene expression

ROT3 ... down-regulated by brassinolide in the wild types studied, an effect that is abolished or extremely reduced by the ucu2 mutations

Pérez-Pérez JM, Ponce MR, Micol JL - The ULTRACURVATA2 gene of Arabidopsis encodes an FK506-binding protein involved in auxin and brassinosteroid signaling

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Gene expression

We found no significant differences in the expression of the NCED3 ... in the wild-type plants after ... BR treatment

Pérez-Pérez JM, Ponce MR, Micol JL - The ULTRACURVATA2 gene of Arabidopsis encodes an FK506-binding protein involved in auxin and brassinosteroid signaling

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Gene expression

We found no significant differences in the expression of the ... BEE1 ... in the wild-type plants after auxin ... treatment

Pérez-Pérez JM, Ponce MR, Micol JL - The ULTRACURVATA2 gene of Arabidopsis encodes an FK506-binding protein involved in auxin and brassinosteroid signaling

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Gene expression

We found no significant differences in the expression of the NCED3 ... in the wild-type plants after auxin ... treatment

Pérez-Pérez JM, Ponce MR, Micol JL - The ULTRACURVATA2 gene of Arabidopsis encodes an FK506-binding protein involved in auxin and brassinosteroid signaling

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Gene expression

We found no significant differences in the expression of the ... UCU1 ... in the wild-type plants after ... BR treatment

Pérez-Pérez JM, Ponce MR, Micol JL - The ULTRACURVATA2 gene of Arabidopsis encodes an FK506-binding protein involved in auxin and brassinosteroid signaling

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Gene expression

We found no significant differences in the expression of the ... UCU1 ... in the wild-type plants after auxin ... treatment

Pérez-Pérez JM, Ponce MR, Micol JL - The ULTRACURVATA2 gene of Arabidopsis encodes an FK506-binding protein involved in auxin and brassinosteroid signaling

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Gene expression

We found no significant differences in the expression of the ... BEE1 ... in the wild-type plants after ... BR treatment

Pérez-Pérez JM, Ponce MR, Micol JL - The ULTRACURVATA2 gene of Arabidopsis encodes an FK506-binding protein involved in auxin and brassinosteroid signaling

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Gene expression

CPD ... found down-regulated by brassinolide in the wild types studied

Pérez-Pérez JM, Ponce MR, Micol JL - The ULTRACURVATA2 gene of Arabidopsis encodes an FK506-binding protein involved in auxin and brassinosteroid signaling

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Gene expression

expression of ... CPD in the ucu1 mutant studied was higher than in the wild type in noninductive conditions

Pérez-Pérez JM, Ponce MR, Micol JL - The ULTRACURVATA2 gene of Arabidopsis encodes an FK506-binding protein involved in auxin and brassinosteroid signaling

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Gene expression

expression of ROT3 ... in the ucu1 mutant studied was higher than in the wild type in noninductive conditions and did not change significantly by auxin or brassinolide treatment

Pérez-Pérez JM, Ponce MR, Micol JL - The ULTRACURVATA2 gene of Arabidopsis encodes an FK506-binding protein involved in auxin and brassinosteroid signaling

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Gene expression

ROT3 ... found down-regulated by brassinolide in the wild types studied

Pérez-Pérez JM, Ponce MR, Micol JL - The ULTRACURVATA2 gene of Arabidopsis encodes an FK506-binding protein involved in auxin and brassinosteroid signaling

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Gene expression

jag-3 ... JAG- VENUS fusion protein is located in the nucleus

Ohno CK, Reddy GV, Heisler MG, Meyerowitz EM - The Arabidopsis JAGGED gene encodes a zinc finger protein that promotes leaf tissue development

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Gene expression

As the cotyledon primordia continue to grow out during the heart stage, JAG mRNA expression is excluded from the tips of the cotyledon primordia but continues to be expressed in a band below this region of repressed expression

Ohno CK, Reddy GV, Heisler MG, Meyerowitz EM - The Arabidopsis JAGGED gene encodes a zinc finger protein that promotes leaf tissue development

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Gene expression

In the torpedo stage embryo, JAG expression continues to be absent at the tip of the cotyledon primordia (Fig. 6C). Cells in the epidermis of the cotyledon have a lower level of JAG transcripts than cells in the inner layers

Ohno CK, Reddy GV, Heisler MG, Meyerowitz EM - The Arabidopsis JAGGED gene encodes a zinc finger protein that promotes leaf tissue development

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Gene expression

JAG transcripts ... are absent from the SAM

Ohno CK, Reddy GV, Heisler MG, Meyerowitz EM - The Arabidopsis JAGGED gene encodes a zinc finger protein that promotes leaf tissue development

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Gene expression

JAG is also expressed in developing leaf primordia of the seedling at 3.5 days and/until 7 days after germination. It is initially expressed throughout the young primordium but later expression becomes excluded from the distal tip of the primordium (Fig. 6D-F). Reduced JAG expression is also detected at the proximal base of the leaf primordia

Ohno CK, Reddy GV, Heisler MG, Meyerowitz EM - The Arabidopsis JAGGED gene encodes a zinc finger protein that promotes leaf tissue development

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Gene expression

JAG mRNA is first detected in late transition stage embryos in cells corresponding to the cotyledon primordia

Ohno CK, Reddy GV, Heisler MG, Meyerowitz EM - The Arabidopsis JAGGED gene encodes a zinc finger protein that promotes leaf tissue development

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Gene expression

jag-5D ... JAG RNA is also detected in the mutant in other tissues in which it is normally not expressed, such as the inflorescence meristem and ovules

Dinneny JR, Yadegari R, Fischer RL, Yanofsky MF, Weigel D - The role of JAGGED in shaping lateral organs

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Gene expression

We confirmed the predicted subcellular localization of JAG with a GFP fusion, which localizes to the nucleus

Dinneny JR, Yadegari R, Fischer RL, Yanofsky MF, Weigel D - The role of JAGGED in shaping lateral organs

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Gene expression

JAG RNA accumulates in the initiating bracts of jag-5D heterozygotes (Fig. 6I), where it is maintained long after emergence of the bracts

Dinneny JR, Yadegari R, Fischer RL, Yanofsky MF, Weigel D - The role of JAGGED in shaping lateral organs

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Gene expression

By RT-PCR, JAG RNA is detected during vegetative and reproductive development in the shoot apex

Dinneny JR, Yadegari R, Fischer RL, Yanofsky MF, Weigel D - The role of JAGGED in shaping lateral organs

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Gene expression

No JAG expression was detected in the petioles

Dinneny JR, Yadegari R, Fischer RL, Yanofsky MF, Weigel D - The role of JAGGED in shaping lateral organs

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Gene expression

JAG mRNA ... is absent from the shoot apical meristem

Dinneny JR, Yadegari R, Fischer RL, Yanofsky MF, Weigel D - The role of JAGGED in shaping lateral organs

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Gene expression

JAG mRNA is detected in emerging leaf primordia

Dinneny JR, Yadegari R, Fischer RL, Yanofsky MF, Weigel D - The role of JAGGED in shaping lateral organs

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Gene expression

not in ... leaves ... JGL mRNA

Dinneny JR, Yadegari R, Fischer RL, Yanofsky MF, Weigel D - The role of JAGGED in shaping lateral organs

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Gene expression

Serial sections through leaf primordia revealed that JAG is predominantly expressed in the blade regions

Dinneny JR, Yadegari R, Fischer RL, Yanofsky MF, Weigel D - The role of JAGGED in shaping lateral organs

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Gene expression

JAG mRNA is localized in the distal region of leaves

Dinneny JR, Yadegari R, Fischer RL, Yanofsky MF, Weigel D - The role of JAGGED in shaping lateral organs

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Gene expression

By RT-PCR, JAG RNA is ... not in ... leaves

Dinneny JR, Yadegari R, Fischer RL, Yanofsky MF, Weigel D - The role of JAGGED in shaping lateral organs

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Gene expression

is detected during vegetative and reproductive development in the shoot apex ... JGL mRNA

Dinneny JR, Yadegari R, Fischer RL, Yanofsky MF, Weigel D - The role of JAGGED in shaping lateral organs

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Gene expression

1-h BR treatments resulted in increased EXO transcript levels in ... wild-type ... plants

Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes

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Gene expression

The KCS1 gene showed weaker expression in untreated dwf1-6 ... plants in comparison to untreated wild-type plants

Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes

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Gene expression

BR-deficient dwf1-6 ... plants showed weaker EXO expression

Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes

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Gene expression

Exp5 ... induced upon 1-h BR treatments in ... dwf1-6 plants

Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes

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Gene expression

Real-time RT-PCR analysis confirmed increased ... δ-TIP transcript levels in soil-grown 35S::EXO T2 generation plants

Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes

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Gene expression

Northern blot analysis confirmed elevated KCS1 ... mRNA levels in T1 generation 35S::EXO plants

Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes

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Gene expression

In agreement with our previous data, increased EXO mRNA levels were found in BR-treated plants (Table 1). Northern blot analysis confirmed the BR induction

Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes

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Gene expression

CPD antisense plants showed weaker EXO expression

Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes

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Gene expression

KCS1 ... decreased mRNA levels in T1 generation EXO-RNAi plants

Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes

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Gene expression

δ-TIP ... induced upon 1-h BR treatments in ... dwf1-6 plants

Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes

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Gene expression

2,4-D (10 nM, 0.1 μM, and 1 μM; 1 h and 3 h) ... did not alter EXO transcript levels

Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes

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Gene expression

AGP4 ... induced upon 1-h BR treatments in wild-type ... plants

Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes

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Gene expression

GA (0.1 μM and 1 μM GA4/GA7; 1 h and 3 h) ... did not alter EXO transcript levels

Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes

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Gene expression

Northern blot analysis confirmed elevated ... Exp5 ... mRNA levels in T1 generation 35S::EXO plants

Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes

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Gene expression

δ-TIP ... induced upon 1-h BR treatments in wild-type ... plants

Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes

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Gene expression

Northern blot analysis confirmed elevated ... AGP4 mRNA levels in T1 generation 35S::EXO plants

Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes

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Gene expression

KCS1 ... induced upon 1-h BR treatments in ... dwf1-6 plants

Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes

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Gene expression

The KCS1 gene showed weaker expression in untreated ... CPD antisense plants in comparison to untreated wild-type plants

Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes

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Gene expression

AGP4 ... decreased mRNA levels in T1 generation EXO-RNAi plants

Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes

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Gene expression

Exp5 ... decreased mRNA levels in T1 generation EXO-RNAi plants

Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes

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Gene expression

1-h BR treatments resulted in increased EXO transcript levels in ... dwf1-6 plants

Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes

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Gene expression

KCS1 ... induced upon 1-h BR treatments in wild-type ... plants

Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes

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Gene expression

AGP4 ... induced upon 1-h BR treatments in ... dwf1-6 plants

Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes

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Gene expression

ethylene precursor ACC (0.1 μM, 1 μM; 1 h and 3 h) did not alter EXO transcript levels

Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes

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Gene expression

Exp5 ... induced upon 1-h BR treatments in wild-type ... plants

Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes

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Gene expression

transgenic WIN1 overexpressors ... KCS1, which is predicted to encode a ketoacyl-CoA synthase involved in wax biosynthesis ... appeared to be up-regulated

Broun P, Poindexter P, Osborne E, Jiang CZ, Riechmann JL - WIN1, a transcriptional activator of epidermal wax accumulation in Arabidopsis

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Gene expression

To determine the effects of WIN1 on gene expression, Northern analysis was performed by using total RNA isolated from leaves of overexpressing lines ... CER1 presented the most striking change in gene expression: its transcript level was clearly correlated with the strength of WIN1 overexpression

Broun P, Poindexter P, Osborne E, Jiang CZ, Riechmann JL - WIN1, a transcriptional activator of epidermal wax accumulation in Arabidopsis

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Gene expression

WIN1 is expressed at higher levels in flowers than in any other tissues. Expression was also detectable, although at lower levels, in stems, siliques, and rosette leaves

Broun P, Poindexter P, Osborne E, Jiang CZ, Riechmann JL - WIN1, a transcriptional activator of epidermal wax accumulation in Arabidopsis

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Gene expression

transgenic WIN1 overexpressors ... CER2, a presumed regulatory protein ... appeared to be up-regulated

Broun P, Poindexter P, Osborne E, Jiang CZ, Riechmann JL - WIN1, a transcriptional activator of epidermal wax accumulation in Arabidopsis

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Gene expression

RNA gel blot analysis indicated that HLR mRNA accumulates in all the organs that we tested (flower bud, stem, leaf and root; data not shown), which is consistent with the pleiotropic phenotype of the hlr mutant. In particular, mRNA accumulated greatly in organs containing meristems, suggesting that the HLR gene works throughout the entire plant, especially in the meristematic regions

Ueda M, Matsui K, Ishiguro S, Sano R, Wada T, Paponov I, Palme K, Okada K - The HALTED ROOT gene encoding the 26S proteasome subunit RPT2a is essential for the maintenance of Arabidopsis meristems

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Gene expression

We then examined expression of the HLR gene in meristems by in situ RNA hybridization experiments ... In the shoot, the HLR gene was expressed uniformly in the SAM in both the vegetative and the reproductive phase

Ueda M, Matsui K, Ishiguro S, Sano R, Wada T, Paponov I, Palme K, Okada K - The HALTED ROOT gene encoding the 26S proteasome subunit RPT2a is essential for the maintenance of Arabidopsis meristems

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Gene expression

hlr mutants ... In 11-day-old mutant seedlings, the WUS expression domain was markedly enlarged, and was expanded to outer and/or inner cell layers (Fig. 5B). Furthermore, WUS mRNA accumulation was sometimes detected in some groups of cells at abnormal places

Ueda M, Matsui K, Ishiguro S, Sano R, Wada T, Paponov I, Palme K, Okada K - The HALTED ROOT gene encoding the 26S proteasome subunit RPT2a is essential for the maintenance of Arabidopsis meristems

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Gene expression

No signal was detected in the crl mutant

Asano T, Yoshioka Y, Kurei S, Sakamoto W, Machida Y; Sodmergen - A mutation of the CRUMPLED LEAF gene that encodes a protein localized in the outer envelope membrane of plastids affects the pattern of cell division, cell differentiation, and plastid division in Arabidopsis

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Gene expression

The CRL gene was ubiquitously expressed

Asano T, Yoshioka Y, Kurei S, Sakamoto W, Machida Y; Sodmergen - A mutation of the CRUMPLED LEAF gene that encodes a protein localized in the outer envelope membrane of plastids affects the pattern of cell division, cell differentiation, and plastid division in Arabidopsis

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Gene expression

CRL protein is localized in the outer envelope membrane of chloroplasts

Asano T, Yoshioka Y, Kurei S, Sakamoto W, Machida Y; Sodmergen - A mutation of the CRUMPLED LEAF gene that encodes a protein localized in the outer envelope membrane of plastids affects the pattern of cell division, cell differentiation, and plastid division in Arabidopsis

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Gene expression

The ROT4 expression was highest in the shoot apices

Narita NN, Moore S, Horiguchi G, Kubo M, Demura T, Fukuda H, Goodrich J, Tsukaya H - Overexpression of a novel small peptide ROTUNDIFOLIA4 decreases cell proliferation and alters leaf shape in Arabidopsis thaliana

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Gene expression

ROT4 ... Expression in the mature cotyledons was extremely low

Narita NN, Moore S, Horiguchi G, Kubo M, Demura T, Fukuda H, Goodrich J, Tsukaya H - Overexpression of a novel small peptide ROTUNDIFOLIA4 decreases cell proliferation and alters leaf shape in Arabidopsis thaliana

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Gene expression

When the ROT4 antisense probe was hybridized to rot4-1D materials, unlike wild type, a strong signal was consistently observed (Figure 4f�l). This agreed with the RT-PCR results, which also indicated higher expression in the rot4-1D mutant than in wild type. In seedlings, there was little expression in the shoot apical meristem (Figure 4f), whereas young leaf primordia showed expression throughout the lamina of young leaf primordia (Figure 4f,g). In cotyledons and older leaves, expression was restricted to vasculature (Figure 4f,g)

Narita NN, Moore S, Horiguchi G, Kubo M, Demura T, Fukuda H, Goodrich J, Tsukaya H - Overexpression of a novel small peptide ROTUNDIFOLIA4 decreases cell proliferation and alters leaf shape in Arabidopsis thaliana

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Gene expression

To determine the intracellular localization of the ROT4 protein, we examined young leaves of 35S::ROT4�GFP transgenic Arabidopsis plants using confocal microscopy ... ROT4�GFP fusion protein was localized to the plasma membrane

Narita NN, Moore S, Horiguchi G, Kubo M, Demura T, Fukuda H, Goodrich J, Tsukaya H - Overexpression of a novel small peptide ROTUNDIFOLIA4 decreases cell proliferation and alters leaf shape in Arabidopsis thaliana

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Gene expression

The ROT4 expression was ... somewhat reduced in the leaf primordia

Narita NN, Moore S, Horiguchi G, Kubo M, Demura T, Fukuda H, Goodrich J, Tsukaya H - Overexpression of a novel small peptide ROTUNDIFOLIA4 decreases cell proliferation and alters leaf shape in Arabidopsis thaliana

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Gene expression

In young seedlings, however, the expression of ROT4 was clearly detected in shoots

Narita NN, Moore S, Horiguchi G, Kubo M, Demura T, Fukuda H, Goodrich J, Tsukaya H - Overexpression of a novel small peptide ROTUNDIFOLIA4 decreases cell proliferation and alters leaf shape in Arabidopsis thaliana

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Gene expression

ROT4 is expressed in wild-type leaves during early developmental stages

Narita NN, Moore S, Horiguchi G, Kubo M, Demura T, Fukuda H, Goodrich J, Tsukaya H - Overexpression of a novel small peptide ROTUNDIFOLIA4 decreases cell proliferation and alters leaf shape in Arabidopsis thaliana

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Gene expression

accumulation of ROT4 mRNA was not detected in mature rosette leaves

Narita NN, Moore S, Horiguchi G, Kubo M, Demura T, Fukuda H, Goodrich J, Tsukaya H - Overexpression of a novel small peptide ROTUNDIFOLIA4 decreases cell proliferation and alters leaf shape in Arabidopsis thaliana

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Gene expression

Vascular systems were also strongly stained throughout the whole plant ... AHK4:GUS

Nishimura C, Ohashi Y, Sato S, Kato T, Tabata S, Ueguchi C - Histidine kinase homologs that act as cytokinin receptors possess overlapping functions in the regulation of shoot and root growth in Arabidopsis

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Gene expression

strong staining was mainly localized in ... growing leaf ... AHK3:GUS

Nishimura C, Ohashi Y, Sato S, Kato T, Tabata S, Ueguchi C - Histidine kinase homologs that act as cytokinin receptors possess overlapping functions in the regulation of shoot and root growth in Arabidopsis

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Gene expression

In various organs, the AHK genes are expressed nearly ubiquitously but at low levels. In seedlings, strong staining was mainly localized in meristematic tissues, such as the SAM ... AHK4:GUS

Nishimura C, Ohashi Y, Sato S, Kato T, Tabata S, Ueguchi C - Histidine kinase homologs that act as cytokinin receptors possess overlapping functions in the regulation of shoot and root growth in Arabidopsis

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Gene expression

strong staining was mainly localized in ... growing leaf ... AHK2:GUS

Nishimura C, Ohashi Y, Sato S, Kato T, Tabata S, Ueguchi C - Histidine kinase homologs that act as cytokinin receptors possess overlapping functions in the regulation of shoot and root growth in Arabidopsis

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Gene expression

AHK3:GUS ... Vascular systems were also strongly stained throughout the whole plant

Nishimura C, Ohashi Y, Sato S, Kato T, Tabata S, Ueguchi C - Histidine kinase homologs that act as cytokinin receptors possess overlapping functions in the regulation of shoot and root growth in Arabidopsis

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Gene expression

AHK2:GUS ... Vascular systems were also strongly stained throughout the whole plant

Nishimura C, Ohashi Y, Sato S, Kato T, Tabata S, Ueguchi C - Histidine kinase homologs that act as cytokinin receptors possess overlapping functions in the regulation of shoot and root growth in Arabidopsis

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Gene expression

In various organs, the AHK genes are expressed nearly ubiquitously but at low levels. In seedlings, strong staining was mainly localized in meristematic tissues, such as the SAM ... AHK2:GUS

Nishimura C, Ohashi Y, Sato S, Kato T, Tabata S, Ueguchi C - Histidine kinase homologs that act as cytokinin receptors possess overlapping functions in the regulation of shoot and root growth in Arabidopsis

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Gene expression

In various organs, the AHK genes are expressed nearly ubiquitously but at low levels. In seedlings, strong staining was mainly localized in meristematic tissues, such as the SAM ... AHK3:GUS

Nishimura C, Ohashi Y, Sato S, Kato T, Tabata S, Ueguchi C - Histidine kinase homologs that act as cytokinin receptors possess overlapping functions in the regulation of shoot and root growth in Arabidopsis

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Gene expression

strong staining was mainly localized in ... growing leaf ... AHK4:GUS

Nishimura C, Ohashi Y, Sato S, Kato T, Tabata S, Ueguchi C - Histidine kinase homologs that act as cytokinin receptors possess overlapping functions in the regulation of shoot and root growth in Arabidopsis

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Gene expression

no PHB expression was detected in AS1>>KAN2 leaves

Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities

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Gene expression

in wild type, PHB mRNA was localized to the SAM

Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities

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Gene expression

PHB was localized to the adaxial regions of wild-type leaves

Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities

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Gene expression

The use of promoter-reporter gene fusions suggested that KAN ... phloem-associated vascular bundle expression

Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities

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Gene expression

PHB was expressed throughout kan1 kan2 kan3 leaf primordia, although there was still a gradient with expression highest towards the adaxial side

Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities

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Gene expression

kan1 kan2 syd ... FIL expression was greatly reduced in this background

Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities

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Gene expression

YAB3 ... β-glucuronidase (GUS) activity was undetectable in the kan1-2 kan2-1 kan3-1 yab3-2

Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities

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Gene expression

in AS1>>KAN2 ... a low level of PHB expression was still present in the apical meristem

Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities

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Gene expression

The use of promoter-reporter gene fusions suggested that KAN1 ... phloem-associated vascular bundle expression

Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities

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Gene expression

heart stage of embryogenesis, KAN1 ... expression pattern in the abaxial basal portion of emerging cotyledon primordia

Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities

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Gene expression

heart stage of embryogenesis ... KAN2 ... expression pattern in the abaxial basal portion of emerging cotyledon primordia

Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities

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Gene expression

KAN1 is expressed in the abaxial regions of all lateral organs

Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities

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Gene expression

in wild type, PHB mRNA ... Later expression was confined to the provascular and vascular tissues of leaves and stems

Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities

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Gene expression

The use of promoter-reporter gene fusions suggested that KAN ... phloem-associated vascular bundle expression

Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities

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Gene expression

kan1 kan2 syd ... PHB was expressed throughout the developing leaves

Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities

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Gene expression

KAN4 appears to have a more limited expression pattern in the ovules

Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities

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Gene expression

YAB3 expression ... in an otherwise wild-type background, GUS activity was detected in the abaxial regions of developing yab3-2 leaves

Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities

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Gene expression

FIL expression was detected throughout young AS1>>KAN2 leaf primordia (Fig. 3I). However, unlike wild-type leaves in which FIL expression is maintained in the marginal abaxial regions (Fig. 3H), FIL expression in AS1>>KAN2 was transient, only being detected in one leaf per apex

Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities

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Gene expression

heart stage of embryogenesis ... KAN3 ... expression pattern in the abaxial basal portion of emerging cotyledon primordia

Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities

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Gene expression

in wild type, PHB mRNA ... was restricted to the adaxial domain as developing primordia separated from the meristem

Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities

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Gene expression

In ... situ hybridization showed that the timing and domain of AG mRNA accumulation in hua1-1 hua2-1 hen3-1 flowers were normal

Wang W, Chen X - HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis

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Gene expression

GUS activity ... showed that HEN3 is expressed in young, presumably dividing, tissue, such as ... young leaves

Wang W, Chen X - HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis

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Gene expression

in hua1-1 hua2-1 hen3-1 flowers ... AG protein abundance was similar between hua1-1 hua2-1 ... flowers

Wang W, Chen X - HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis

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Gene expression

in the ... hua1-1 hua2-1 hen3-1 flowers ... The abundance of ... AP2 ... was also increased

Wang W, Chen X - HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis

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Gene expression

in the ... hua1-1 hua2-1 hen3-1 flowers ... The abundance of AP1 ... was also increased

Wang W, Chen X - HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis

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Gene expression

hua1-1 hua2-1 hen3-1 flowers were defective in the spatial control of AP1 expression

Wang W, Chen X - HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis

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Gene expression

We performed in situ hybridization to determine the localization of HEN3 RNA in inflorescence meristems and in flowers. HEN3 RNA was present throughout the inflorescence meristem, the inflorescence stem and young flowers

Wang W, Chen X - HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis

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Gene expression

In situ hybridization showed that histone H4, a gene known to be expressed in a cell cycle-dependent manner, was expressed similarly in wild-type and hen3-1 SAMs

Wang W, Chen X - HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis

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Gene expression

hua1-1 hua2-1 hen3-1 flowers were defective in the ... temporal control of WUS expression in flower development

Wang W, Chen X - HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis

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Gene expression

RNA filter hybridization showed that HEN3 RNA was present in ... stems

Wang W, Chen X - HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis

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Gene expression

GUS activity showed that HEN3 is expressed in young, presumably dividing, tissue, such as shoot ... tips

Wang W, Chen X - HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis

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Gene expression

HEN3 RNA ... was more abundant in ag-3 inflorescences

Wang W, Chen X - HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis

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Gene expression

GUS activity ... showed that HEN3 is expressed in young, presumably dividing, tissue, such as ... flowers

Wang W, Chen X - HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis

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Gene expression

We performed RNA filter hybridization to determine whether the hen3-1 mutation also results in reduced AG RNA levels in the hua1-1 hua2-1 background ... Three AG RNAs, RNA1, RNA2 and AG mRNA, can be detected in hua1-1 hua2-1 and hua1-1 hua2-1 hen3-1 flowers ... hen3-1 resulted in an increase in abundance in both the intron-containing RNAs and the mRNA in the hua1-1 hua2-1 background

Wang W, Chen X - HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis

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Gene expression

RNA filter hybridization showed that HEN3 RNA was present in leaves

Wang W, Chen X - HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis

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Gene expression

In situ hybridization showed that histone H4, a gene known to be expressed in a cell cycle-dependent manner, was expressed similarly in wild-type and hen3-1 ... leaf primordia

Wang W, Chen X - HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis

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Gene expression

RNA filter hybridization showed that HEN3 RNA was present in ... inflorescences

Wang W, Chen X - HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis

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Gene expression

The expression of the above- mentioned genes was quantified by real-time PCR in expanding leaves collected from ron2-1 ... homozygous plants ... AG expression was measured on the leaf samples described above and no significant differences with the wild type were found

Cnops G, Jover-Gil S, Peters JL, Neyt P, De Block S, Robles P, Ponce MR, Gerats T, Micol JL, Van Lijsebettens M - The rotunda2 mutants identify a role for the LEUNIG gene in vegetative leaf morphogenesis

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Gene expression

The expression of the above- mentioned genes was quantified by real-time PCR in expanding leaves collected from ... ron2-2 ... homozygous plants ... AS1 ... transcript levels were normal in the mutants and did not account for the reduction in STM transcript level

Cnops G, Jover-Gil S, Peters JL, Neyt P, De Block S, Robles P, Ponce MR, Gerats T, Micol JL, Van Lijsebettens M - The rotunda2 mutants identify a role for the LEUNIG gene in vegetative leaf morphogenesis

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Gene expression

The expression of the above- mentioned genes was quantified by real-time PCR in expanding leaves collected from ... ron2-2 ... homozygous plants ... STM expression level in each mutant tested was reduced 10–20-fold compared with that of the wild type

Cnops G, Jover-Gil S, Peters JL, Neyt P, De Block S, Robles P, Ponce MR, Gerats T, Micol JL, Van Lijsebettens M - The rotunda2 mutants identify a role for the LEUNIG gene in vegetative leaf morphogenesis

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Gene expression

The expression of the above- mentioned genes was quantified by real-time PCR in expanding leaves collected from ... lug- 1 homozygous plants ... AS2 transcript levels were normal in the mutants and did not account for the reduction in STM transcript level

Cnops G, Jover-Gil S, Peters JL, Neyt P, De Block S, Robles P, Ponce MR, Gerats T, Micol JL, Van Lijsebettens M - The rotunda2 mutants identify a role for the LEUNIG gene in vegetative leaf morphogenesis

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Gene expression

The expression of the above- mentioned genes was quantified by real-time PCR in expanding leaves collected from ron2-1 ... homozygous plants ... STM expression level in each mutant tested was reduced 10–20-fold compared with that of the wild type

Cnops G, Jover-Gil S, Peters JL, Neyt P, De Block S, Robles P, Ponce MR, Gerats T, Micol JL, Van Lijsebettens M - The rotunda2 mutants identify a role for the LEUNIG gene in vegetative leaf morphogenesis

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Gene expression

The expression of the above- mentioned genes was quantified by real-time PCR in expanding leaves collected from ... ron2-2 ... homozygous plants ... AS2 transcript levels were normal in the mutants and did not account for the reduction in STM transcript level

Cnops G, Jover-Gil S, Peters JL, Neyt P, De Block S, Robles P, Ponce MR, Gerats T, Micol JL, Van Lijsebettens M - The rotunda2 mutants identify a role for the LEUNIG gene in vegetative leaf morphogenesis

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Gene expression

The expression of the above- mentioned genes was quantified by real-time PCR in expanding leaves collected from ron2-1 ... homozygous plants ... AS2 transcript levels were normal in the mutants and did not account for the reduction in STM transcript level

Cnops G, Jover-Gil S, Peters JL, Neyt P, De Block S, Robles P, Ponce MR, Gerats T, Micol JL, Van Lijsebettens M - The rotunda2 mutants identify a role for the LEUNIG gene in vegetative leaf morphogenesis

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Gene expression

The expression of the above- mentioned genes was quantified by real-time PCR in expanding leaves collected from ... ron2-2 ... homozygous plants ... AG expression was measured on the leaf samples described above and no significant differences with the wild type were found

Cnops G, Jover-Gil S, Peters JL, Neyt P, De Block S, Robles P, Ponce MR, Gerats T, Micol JL, Van Lijsebettens M - The rotunda2 mutants identify a role for the LEUNIG gene in vegetative leaf morphogenesis

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Gene expression

The expression of the above- mentioned genes was quantified by real-time PCR in expanding leaves collected from ... lug- 1 homozygous plants ... AS1 ... transcript levels were normal in the mutants and did not account for the reduction in STM transcript level

Cnops G, Jover-Gil S, Peters JL, Neyt P, De Block S, Robles P, Ponce MR, Gerats T, Micol JL, Van Lijsebettens M - The rotunda2 mutants identify a role for the LEUNIG gene in vegetative leaf morphogenesis

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Gene expression

The expression of the above- mentioned genes was quantified by real-time PCR in expanding leaves collected from ... lug- 1 homozygous plants ... AG expression was measured on the leaf samples described above and no significant differences with the wild type were found

Cnops G, Jover-Gil S, Peters JL, Neyt P, De Block S, Robles P, Ponce MR, Gerats T, Micol JL, Van Lijsebettens M - The rotunda2 mutants identify a role for the LEUNIG gene in vegetative leaf morphogenesis

Visit the PubMed Article

Gene expression

The expression of the above- mentioned genes was quantified by real-time PCR in expanding leaves collected from ... lug- 1 homozygous plants ... STM expression level in each mutant tested was reduced 10–20-fold compared with that of the wild type

Cnops G, Jover-Gil S, Peters JL, Neyt P, De Block S, Robles P, Ponce MR, Gerats T, Micol JL, Van Lijsebettens M - The rotunda2 mutants identify a role for the LEUNIG gene in vegetative leaf morphogenesis

Visit the PubMed Article

Gene expression

The expression of the above- mentioned genes was quantified by real-time PCR in expanding leaves collected from ron2-1 ... homozygous plants ... AS1 ... transcript levels were normal in the mutants and did not account for the reduction in STM transcript level

Cnops G, Jover-Gil S, Peters JL, Neyt P, De Block S, Robles P, Ponce MR, Gerats T, Micol JL, Van Lijsebettens M - The rotunda2 mutants identify a role for the LEUNIG gene in vegetative leaf morphogenesis

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Gene expression

level of HLS1 protein was increased in response to ethylene treatment

Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis

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Gene expression

The in situ localization of ARF2 mRNA in hls1-1 mutants also revealed that the expression patterns of ARF2 in cotyledons and hypocotyl were not affected by the hls1-1 mutation

Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis

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Gene expression

level of ARF2 was ... not affected in hls1-1 mutant upon exposure to light

Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis

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Gene expression

Our studies show that ARF2 protein levels were unchanged by treatment with various concentrations of auxin (Figure 4C), while auxin-inducible IAA1 mRNA was increased significantly by the same auxin treatment. These results suggest that the abundance of the ARF2 is not mediated by an indirect effect of ethylene on auxin levels

Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis

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Gene expression

Transgenic seedlings that overexpress HLS1 have slightly lower ARF2 protein levels than that observed in wild-type

Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis

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Gene expression

ARF2 also showed expression in the cotyledons and the hypocotyl after hook opening upon exposure of etiolated seedlings to light

Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis

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Gene expression

level of ARF2 protein was decreased upon exposure to ethylene

Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis

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Gene expression

level of HLS1 transcription is increased by ethylene (Lehman et al., 1996)

Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis

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Gene expression

ARF2 mRNA was found to be present in ... etiolated seedlings ... in the ... leaf primordia

Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis

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Gene expression

ARF2 mRNA was found to be present in ... etiolated seedlings ... in the cotyledons

Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis

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Gene expression

Immunoblot analyses of etiolated seedlings that were treated with ACC with or without MG132, a specific inhibitor of the proteasome-dependent protein degradation, revealed that MG132 suppressed the ethylene-dependent decrease in ARF2 protein levels (Figure 3C). These results strongly suggest that ethylene-mediated degradation of the ARF2 protein is via a proteasome-dependent pathway

Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis

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Gene expression

Interestingly, the arf2-3 mutation partially restored DR5::GUS expression within cells located on the concave side of the hook in the arf2-3 hls1-1 double mutant in the presence of ethylene

Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis

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Gene expression

histochemical staining of transgenic seedlings expressing pARF2::GUS:ARF2 in wild-type, hls1 mutant, and light-grown wild-type seedlings revealed that the ARF2 protein was mainly expressed in the apical portion of the hypocotyl and in the cotyledons, and these patterns were not affected by the hls1-1 mutation or exposure to light (Figure 2F)

Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis

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Gene expression

histochemical staining of transgenic seedlings expressing pARF2::GUS:ARF2 in wild-type, hls1 mutant, and light-grown wild-type seedlings revealed that the ARF2 protein was mainly expressed in the apical portion of the hypocotyl and in the cotyledons, and these patterns were not affected by the hls1-1 mutation or exposure to light (Figure 2F)

Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis

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Gene expression

Immunoblot analyses revealed that the level of HLS1 protein was decreased upon light exposure, even in the presence of the ethylene precursor ACC

Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis

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Gene expression

ARF2 mRNA was found to be present in the apical hook region of etiolated seedlings

Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis

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Gene expression

Since neither expression patterns nor transcription levels of ARF2 (data not shown) were affected by the loss of HLS1 function

Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis

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Gene expression

DR5::GUS was only expressed within cells located at the concave side of the hypocotyl hook (Figure 5), and the level of expression was enhanced by ethylene treatment. However, the expression level in the cotyledon was highly variable. In hls1-1 mutants, this highly localized expression pattern was completely abolished

Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis

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Gene expression

level of ARF2 was increased in wild-type seedlings ... upon exposure to light

Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis

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Gene expression

Polyclonal antibodies that specifically recognize the ARF2 protein were generated (Figure 3A). Immunoblot analysis was used to detect ARF2 protein levels in 3-day-old etiolated seedlings. Consistent with the transgenic expression of pARF2::GUS:ARF2 in wild-type and hls1-1 mutant, the level of ARF2 protein was increased in the hls1-1 mutant

Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis

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Gene expression

Interestingly, the intensity of GUS staining in etiolated hls1 mutants and in wild-type seedlings exposed to light was higher than that observed in etiolated wild-type seedlings (Figure 2F), suggesting that the level of GUS:ARF2 protein may be regulated by HLS1 functions

Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis

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Gene expression

arf2-3 had no effect on the expression pattern of DR5::GUS in wild-type plants

Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis

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Gene expression

In older leaves (rosette) as well as in cauline leaves, SHN3 was mainly expressed in the central vein with lower expression in the entire blade

Aharoni A, Dixit S, Jetter R, Thoenes E, van Arkel G, Pereira A - The SHINE clade of AP2 domain transcription factors activates wax biosynthesis, alters cuticle properties, and confers drought tolerance when overexpressed in Arabidopsis

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Gene expression

In older leaves (rosette) as well as in cauline leaves, SHN3 was mainly expressed in the central vein with lower expression in the entire blade

Aharoni A, Dixit S, Jetter R, Thoenes E, van Arkel G, Pereira A - The SHINE clade of AP2 domain transcription factors activates wax biosynthesis, alters cuticle properties, and confers drought tolerance when overexpressed in Arabidopsis

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Gene expression

SHN1/WIN1 ... constructs, which linked 2.0-kb DNA sequences upstream of the predicted ATG codon of each gene to the β-glucuronidase (GUS) reporter gene ... SHN1/WIN1 expression was detected in the inflorescence and root tissues but not in stem, mature rosette leaves, or cauline leaves

Aharoni A, Dixit S, Jetter R, Thoenes E, van Arkel G, Pereira A - The SHINE clade of AP2 domain transcription factors activates wax biosynthesis, alters cuticle properties, and confers drought tolerance when overexpressed in Arabidopsis

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Gene expression

SHN1/WIN1 ... constructs, which linked 2.0-kb DNA sequences upstream of the predicted ATG codon of each gene to the β-glucuronidase (GUS) reporter gene ... GUS expression was observed ... in ... support cells of their trichomes

Aharoni A, Dixit S, Jetter R, Thoenes E, van Arkel G, Pereira A - The SHINE clade of AP2 domain transcription factors activates wax biosynthesis, alters cuticle properties, and confers drought tolerance when overexpressed in Arabidopsis

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Gene expression

SHN1/WIN1 ... constructs, which linked 2.0-kb DNA sequences upstream of the predicted ATG codon of each gene to the β-glucuronidase (GUS) reporter gene ... GUS expression was observed ... in ... very young leaves in the rosette

Aharoni A, Dixit S, Jetter R, Thoenes E, van Arkel G, Pereira A - The SHINE clade of AP2 domain transcription factors activates wax biosynthesis, alters cuticle properties, and confers drought tolerance when overexpressed in Arabidopsis

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Gene expression

The SHN3 gene was most broadly expressed and was active in all plant organs. It showed expression in the vasculature and in the lateral root tip (Figure 9). When staining young 10-d-old seedlings, expression was detected in the support cells of trichomes present on the most newly formed leaves

Aharoni A, Dixit S, Jetter R, Thoenes E, van Arkel G, Pereira A - The SHINE clade of AP2 domain transcription factors activates wax biosynthesis, alters cuticle properties, and confers drought tolerance when overexpressed in Arabidopsis

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Gene expression

RNA gel blot analysis of total RNAs, isolated from mock- or brassinolide-treated seedlings of wild-type ... revealed no detectable difference in the abundance of the TTL transcript between mock- and BR-treated seedlings

Nam KH, Li J - The Arabidopsis transthyretin-like protein is a potential substrate of BRASSINOSTEROID-INSENSITIVE 1

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Gene expression

To obtain a better picture of tissue specificity of TTL gene expression, we constructed a TTL-β-glucuronidase (GUS) reporter gene by fusing a 1.6-kb genomic fragment containing the native TTL promoter to a GUS reporter gene. As shown in Figures 3C to 3F, the GUS activity was detected mainly in ... leaf primordia

Nam KH, Li J - The Arabidopsis transthyretin-like protein is a potential substrate of BRASSINOSTEROID-INSENSITIVE 1

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Gene expression

To obtain a better picture of tissue specificity of TTL gene expression, we constructed a TTL-β-glucuronidase (GUS) reporter gene by fusing a 1.6-kb genomic fragment containing the native TTL promoter to a GUS reporter gene. As shown in Figures 3C to 3F, the GUS activity was detected mainly in ... newly expanding young leaves

Nam KH, Li J - The Arabidopsis transthyretin-like protein is a potential substrate of BRASSINOSTEROID-INSENSITIVE 1

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Gene expression

Confocal microscopic analysis of the resulting TTL:GFP transgenic plants revealed a similar green fluorescent pattern between TTL:GFP and BRI1:GFP, indicating that TTL was indeed associated with the plasma membrane ... The membrane association of TTL was also confirmed by a cell fractionation assay. Membrane proteins of a transgenic TTL:GFP plant were separated from cytosolic proteins by differential centrifugation, these proteins were separated by SDS-PAGE, and the presence of TTL:GFP in the two fractions was detected by immunoblotting using a commercial anti-GFP antiserum. As shown in Figure 4B, both BRI1:GFP and TTL:GFP were mainly in the membrane fraction

Nam KH, Li J - The Arabidopsis transthyretin-like protein is a potential substrate of BRASSINOSTEROID-INSENSITIVE 1

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Gene expression

TTL was expressed in all the tissues that we analyzed, with the flower buds and elongating inflorescence stems accumulating the highest levels of the TTL transcripts

Nam KH, Li J - The Arabidopsis transthyretin-like protein is a potential substrate of BRASSINOSTEROID-INSENSITIVE 1

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Gene expression

GIF1 was ... expressed ... weakly in leaves

Kim JH, Kende H - A transcriptional coactivator, AtGIF1, is involved in regulating leaf growth and morphology in Arabidopsis

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Gene expression

Onion epidermal cells transformed transiently to express GIF1 fused to enhanced yellow fluorescent protein (EYFP) also showed nuclear speckles, which were distributed throughout the nucleus in 21 of 40 transformants

Kim JH, Kende H - A transcriptional coactivator, AtGIF1, is involved in regulating leaf growth and morphology in Arabidopsis

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Gene expression

GIF1 was strongly expressed in ... shoot tips containing the shoot apical meristem

Kim JH, Kende H - A transcriptional coactivator, AtGIF1, is involved in regulating leaf growth and morphology in Arabidopsis

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Gene expression

rdr6-11 ... expression was increased ... SPL3

Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS - SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis

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Gene expression

rdr6-11 ... we examined the accumulation of miR156 ... Little or no difference in the levels of these miRNAs was observed

Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS - SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis

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Gene expression

At5g18040 ... mRNA accumulates in sgs3-11 and rdr6-11, but not in zip-1

Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS - SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis

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Gene expression

sgs3-11 ... expression was increased ... of ARF3/ETTIN

Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS - SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis

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Gene expression

rdr6-11 ... expression was increased ... of ARF3/ETTIN

Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS - SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis

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Gene expression

zip-1 ... expression was increased ... of ... SPL3

Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS - SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis

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Gene expression

zip-1 ... expression was increased ... of ARF3/ETTIN

Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS - SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis

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Gene expression

At1g63130 ... mRNA accumulates in sgs3-11 and rdr6-11, but not in zip-1

Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS - SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis

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Gene expression

zip-1 ... expression was increased ... of ... ARF4

Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS - SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis

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Gene expression

At1g63130 ... mRNA accumulates in sgs3-11

Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS - SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis

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Gene expression

At5g18040 ... mRNA accumulates in sgs3-11

Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS - SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis

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Gene expression

sgs3-11 ... expression was increased ... of ... ARF4

Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS - SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis

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Gene expression

At5g18040 ... mRNA accumulates in ... rdr6-11

Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS - SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis

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Gene expression

sgs3-11 ... expression was increased ... of ... SPL3

Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS - SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis

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Gene expression

rdr6-11 ... expression was increased ... ARF4

Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS - SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis

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Gene expression

At1g63130 ... mRNA accumulates in ... rdr6-11

Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS - SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis

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Gene expression

sgs3-11 ... we examined the accumulation of miR156 ... Little or no difference in the levels of these miRNAs was observed

Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS - SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis

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Gene expression

zip-1 ... we examined the accumulation of miR156 ... Little or no difference in the levels of these miRNAs was observed

Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS - SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis

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Gene expression

In contrast with ATB2 and LHY, several other leaders tested drove translation as effectively in eif3h-1 as in the wild type; these included the leaders of ABSCISIC ACID INSENSITIVE5 (ABI5), the omega (Ω) leader of Tobacco mosaic virus (TMV), the small subunit of Rubisco (RBCS), and the bZip protein HY5 (Figure 7G). A moderate, yet significant (P < 0.05) difference in expression was observed for chlorophyll a/b binding protein 2 (CAB2

Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h

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Gene expression

we examined whether and how eIF3h might affect the translation of specific mRNAs. Selected 5′ leader sequences were compared for their translational capacity between eif3h mutants and the wild type using a dual-luciferase reporter assay ... the activity of the FLUC reporter gene fused to the ATB2 leader was reduced fourfold, on average, in the eif3h-1 mutant compared with the wild type

Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h

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Gene expression

we examined whether and how eIF3h might affect the translation of specific mRNAs. Selected 5′ leader sequences were compared for their translational capacity between eif3h mutants and the wild type using a dual-luciferase reporter assay ... the activity of the FLUC reporter gene fused to the ATB2 leader was reduced fourfold, on average, in the eif3h-1 mutant compared with the wild type ... Inhibition of translation was also observed for the leader of the myb protein LATE ELONGATED HYPOCOTYL (LHY

Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h

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Gene expression

Vice versa, no difference was detected in the level of two eIF3 subunits, eIF3b and eIF3h, in csn1 or csn7 mutants

Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h

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Gene expression

In contrast with ATB2 and LHY, several other leaders tested drove translation as effectively in eif3h-1 as in the wild type; these included the leaders of ABSCISIC ACID INSENSITIVE5 (ABI5), the omega (Ω) leader of Tobacco mosaic virus (TMV), the small subunit of Rubisco (RBCS), and the bZip protein HY5

Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h

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Gene expression

In wild-type plants, the translational activity of the ATB2:FLUC reporter was specifically repressed by sucrose (Figure 9C), consistent with previous conclusions (Rook et al., 1998a); for comparison, the HY5 leader was insensitive to sucrose

Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h

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Gene expression

Morphological phenotypes of the eif3h-1 mutant ... suggested an altered auxin response. Consistent with this notion, the mRNA levels of two auxin-inducible genes were elevated slightly in the absence (IAA3 and IAA14) or the presence (IAA3) of added auxin, whereas the level of IAA17 remained normal (Figure 3G

Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h

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Gene expression

In contrast with ATB2 and LHY, several other leaders tested drove translation as effectively in eif3h-1 as in the wild type; these included the leaders of ABSCISIC ACID INSENSITIVE5 (ABI5), the omega (Ω) leader of Tobacco mosaic virus (TMV), the small subunit of Rubisco (RBCS), and the bZip protein HY5

Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h

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Gene expression

Vice versa, no difference was detected in the level of two eIF3 subunits, eIF3b and eIF3h, in csn1 or csn7 mutants

Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h

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Gene expression

To seek independent evidence for a role of eIF3h in translation initiation on specific mRNAs, we compared eif3h-1 mutants and the wild type for the distribution of specific mRNAs in sucrose gradients designed to fractionate polysomal and nonpolysomal RNA, followed by detection of specific transcripts by RT-PCR ... a reduction in the polysome loading of the endogenous ATB2 mRNA was detected in the eif3h-1 mutant

Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h

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Gene expression

Morphological phenotypes of the eif3h-1 mutant ... suggested an altered auxin response. Consistent with this notion, the mRNA levels of two auxin-inducible genes were elevated slightly in the absence (IAA3 and IAA14) or the presence (IAA3) of added auxin, whereas the level of IAA17 remained normal (Figure 3G

Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h

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Gene expression

Vice versa, no difference was detected in the level of two eIF3 subunits, eIF3b and eIF3h, in csn1 or csn7 mutants

Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h

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Gene expression

Vice versa, no difference was detected in the level of two eIF3 subunits, eIF3b and eIF3h, in csn1 or csn7 mutants

Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h

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Gene expression

To investigate the role of eIF3h in Arabidopsis, we isolated a recessive mutant allele (eif3h-1) carrying a T-DNA insertion in the 10th exon (Figure 2A). A truncated eIF3h-related protein was detected in this allele

Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h

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Gene expression

Although eIF3h could associate with the CSN (Karniol et al., 1998), loss of eIF3h did not affect accumulation of its CSN4 or CSN5 subunits (Figure 4C

Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h

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Gene expression

Morphological phenotypes of the eif3h-1 mutant ... suggested an altered auxin response. Consistent with this notion, the mRNA levels of two auxin-inducible genes were elevated slightly in the absence (IAA3 and IAA14) or the presence (IAA3) of added auxin, whereas the level of IAA17 remained normal (Figure 3G

Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h

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Gene expression

In wild-type plants, the translational activity of the ATB2:FLUC reporter was specifically repressed by sucrose (Figure 9C), consistent with previous conclusions (Rook et al., 1998a); for comparison, the HY5 leader was insensitive to sucrose

Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h

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Gene expression

To seek independent evidence for a role of eIF3h in translation initiation on specific mRNAs, we compared eif3h-1 mutants and the wild type for the distribution of specific mRNAs in sucrose gradients designed to fractionate polysomal and nonpolysomal RNA, followed by detection of specific transcripts by RT-PCR ... For comparison, the rbcS mRNA did not significantly change its distribution between polysomal and nonpolysomal fractions

Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h

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Gene expression

Although eIF3h could associate with the CSN (Karniol et al., 1998), loss of eIF3h did not affect accumulation of its CSN4 or CSN5 subunits (Figure 4C

Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h

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Gene expression

The eIF3h protein accumulated in all organs tested yet was most abundant in roots and flowers

Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h

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Gene expression

Morphological phenotypes of the eif3h-1 mutant ... suggested an altered auxin response. Consistent with this notion, the mRNA levels of two auxin-inducible genes were elevated slightly in the absence (IAA3 and IAA14) or the presence (IAA3) of added auxin, whereas the level of IAA17 remained normal (Figure 3G

Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h

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Gene expression

In contrast with ATB2 and LHY, several other leaders tested drove translation as effectively in eif3h-1 as in the wild type; these included the leaders of ABSCISIC ACID INSENSITIVE5 (ABI5), the omega (Ω) leader of Tobacco mosaic virus (TMV), the small subunit of Rubisco (RBCS), and the bZip protein HY5

Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h

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Gene expression

An eIF3h protein truncated by ∼2 kD accumulated in the eif3h-2 mutant

Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h

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Gene expression

BOP1 ... is expressed at very high levels in the petiole relative to the blade

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE1 encodes a BTB/POZ domain protein required for leaf morphogenesis in Arabidopsis thaliana

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Gene expression

BOP1 transcripts are also present along the margin of young leaves, where the adaxial and abax- ial domains juxtapose

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE1 encodes a BTB/POZ domain protein required for leaf morphogenesis in Arabidopsis thaliana

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Gene expression

After germination, BOP1 transcripts ... are present in the rosette leaf primordia

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE1 encodes a BTB/POZ domain protein required for leaf morphogenesis in Arabidopsis thaliana

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Gene expression

The first stage at which BOP1 transcripts can be relia- bly detected is the torpedo stage of embryogenesis, when BOP1 is found in two discrete foci at the base of the develop- ing cotyledons, specifically on the adaxial side near the bound- ary with the shoot apical meristem

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE1 encodes a BTB/POZ domain protein required for leaf morphogenesis in Arabidopsis thaliana

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Gene expression

BOP1 appears to be transcribed at the same relative level in whole rosette leaves regardless of age

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE1 encodes a BTB/POZ domain protein required for leaf morphogenesis in Arabidopsis thaliana

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Gene expression

In maturing leaves, a low level of BOP1 expression becomes detectable across the adaxial por- tion of the leaf

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE1 encodes a BTB/POZ domain protein required for leaf morphogenesis in Arabidopsis thaliana

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Gene expression

As the leaves grow out, BOP1 is expressed strongly yet in a highly restricted pattern at the base of the leaf primordia on the adaxial side adjacent to the SAM

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE1 encodes a BTB/POZ domain protein required for leaf morphogenesis in Arabidopsis thaliana

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Gene expression

In bop1-1 mutant plants, BOP1 transcription is elevated in all tissues compared with the wild type and becomes readily detectable in cotyledons and cauline leaves (Fig. 5B), consistent with the manifestation of phenotypes in these tissues

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE1 encodes a BTB/POZ domain protein required for leaf morphogenesis in Arabidopsis thaliana

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Gene expression

After germination, BOP1 transcripts are not observed in the SAM

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE1 encodes a BTB/POZ domain protein required for leaf morphogenesis in Arabidopsis thaliana

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Gene expression

BOP1 is expressed at barely detectable levels in cotyledons

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE1 encodes a BTB/POZ domain protein required for leaf morphogenesis in Arabidopsis thaliana

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Gene expression

upon AtE2Fa over-expression ... we also observed ele- vated expression of CycB1, a mitotic cyclin, sug- gesting the involvement of AtE2Fa for further cell cycle progression

He SS, Liu J, Xie Z, O'Neill D, Dotson S - Arabidopsis E2Fa plays a bimodal role in regulating cell division and cell growth

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Gene expression

upon AtE2Fa over-expression ... we found that the expression of several cell cycle inhibitor genes, including ... KRP3 ... was also up- regulated

He SS, Liu J, Xie Z, O'Neill D, Dotson S - Arabidopsis E2Fa plays a bimodal role in regulating cell division and cell growth

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Gene expression

The positive cell cycle factors up- regulated upon AtE2Fa over-expression included genes for G1/S transition (CycD2)

He SS, Liu J, Xie Z, O'Neill D, Dotson S - Arabidopsis E2Fa plays a bimodal role in regulating cell division and cell growth

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Gene expression

The positive cell cycle factors up- regulated upon AtE2Fa over-expression included genes for ... DNA synthesis (RNR1

He SS, Liu J, Xie Z, O'Neill D, Dotson S - Arabidopsis E2Fa plays a bimodal role in regulating cell division and cell growth

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Gene expression

upon AtE2Fa over-expression ... we found that the expression of several cell cycle inhibitor genes, including RB1 ... was also up- regulated

He SS, Liu J, Xie Z, O'Neill D, Dotson S - Arabidopsis E2Fa plays a bimodal role in regulating cell division and cell growth

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Gene expression

upon AtE2Fa over-expression ... we found that the expression of several cell cycle inhibitor genes, including ... KRP5, was also up- regulated

He SS, Liu J, Xie Z, O'Neill D, Dotson S - Arabidopsis E2Fa plays a bimodal role in regulating cell division and cell growth

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Gene expression

Comparison of the expression levels in bulked leaf samples revealed that, while the expression of ATPK1 was not changed ... upon the over-expression of AtE2Fa

He SS, Liu J, Xie Z, O'Neill D, Dotson S - Arabidopsis E2Fa plays a bimodal role in regulating cell division and cell growth

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Gene expression

The positive cell cycle factors up- regulated upon AtE2Fa over-expression included genes for ... DNA synthesis ... PCNA

He SS, Liu J, Xie Z, O'Neill D, Dotson S - Arabidopsis E2Fa plays a bimodal role in regulating cell division and cell growth

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Gene expression

Comparison of the expression levels in bulked leaf samples revealed that ... the expression of ATPK19 was increased upon the over-expression of AtE2Fa

He SS, Liu J, Xie Z, O'Neill D, Dotson S - Arabidopsis E2Fa plays a bimodal role in regulating cell division and cell growth

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Gene expression

In wild-type first leaves, DR5:GUS appears 3 DAG in a single cell at the leaf distal tip (Table 4, Figure 5A). In mutant first leaves, DR5:GUS is evident with similar timing. However, the position is often asymmetric relative to the leaf, shifted to one side of the distal tip (Table 4, Figures 5B and 5C). Subsequent DR5:GUS expression in mutant leaf blades is slightly later than in the wild type (Table 4), and no differences in pattern are evident, although expression within the blade at these stages is very transient, so that pattern differences would be difficult to detect (Figures 5D to 5F). At this stage, distal tip expression coincides with the distal margin gap, and in mutant leaves, both sometimes appear asymmetrically placed (Figure 5E). Asymmetric DR5:GUS expression is especially evident when expression begins in hydathodes (Table 4). In wild-type first leaves, hydathode expression usually appears in two points at equivalent positions along the leaf margin (Figure 5G). By contrast, expression in both as1-16 and as2-1 may begin at just one point and be followed by expression in another one or two, often unequally positioned points in as2-1 leaves (Figures 5H and 5I). Expression of DR5:GUS at this stage coincides with margin gaps (Figures 5J to 5L), and the margin gaps seem to contain more cells in the mutants than the wild type (cf. Figures 5K and 5L to 5J

Zgurski JM, Sharma R, Bolokoski DA, Schultz EA - Asymmetric auxin response precedes asymmetric growth and differentiation of asymmetric leaf1 and asymmetric leaf2 Arabidopsis leaves

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Gene expression

Consistent with basipetal decrease in cell division (Donnelly et al., 1999), at 5 DAG, cycB1:GUS expression is diminishing in the distal half of both wild-type and mutant leaves, and the number of cells expressing is again lower in as2-1

Zgurski JM, Sharma R, Bolokoski DA, Schultz EA - Asymmetric auxin response precedes asymmetric growth and differentiation of asymmetric leaf1 and asymmetric leaf2 Arabidopsis leaves

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Gene expression

At 4 DAG, cycB1:GUS ... number of cells expressing the reporter gene is reduced in both halves of as2-1 leaves

Zgurski JM, Sharma R, Bolokoski DA, Schultz EA - Asymmetric auxin response precedes asymmetric growth and differentiation of asymmetric leaf1 and asymmetric leaf2 Arabidopsis leaves

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Gene expression

In wild-type first leaves, DR5:GUS appears 3 DAG in a single cell at the leaf distal tip (Table 4, Figure 5A). In mutant first leaves, DR5:GUS is evident with similar timing. However, the position is often asymmetric relative to the leaf, shifted to one side of the distal tip (Table 4, Figures 5B and 5C). Subsequent DR5:GUS expression in mutant leaf blades is slightly later than in the wild type (Table 4), and no differences in pattern are evident, although expression within the blade at these stages is very transient, so that pattern differences would be difficult to detect (Figures 5D to 5F). At this stage, distal tip expression coincides with the distal margin gap, and in mutant leaves, both sometimes appear asymmetrically placed (Figure 5E). Asymmetric DR5:GUS expression is especially evident when expression begins in hydathodes (Table 4). In wild-type first leaves, hydathode expression usually appears in two points at equivalent positions along the leaf margin (Figure 5G). By contrast, expression in both as1-16 and as2-1 may begin at just one point and be followed by expression in another one or two, often unequally positioned points in as2-1 leaves (Figures 5H and 5I). Expression of DR5:GUS at this stage coincides with margin gaps (Figures 5J to 5L), and the margin gaps seem to contain more cells in the mutants than the wild type (cf. Figures 5K and 5L to 5J

Zgurski JM, Sharma R, Bolokoski DA, Schultz EA - Asymmetric auxin response precedes asymmetric growth and differentiation of asymmetric leaf1 and asymmetric leaf2 Arabidopsis leaves

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Gene expression

At 4 DAG, cycB1:GUS expression is similar in distal and proximal leaf halves and shows no significant difference between the wild type and as1-16

Zgurski JM, Sharma R, Bolokoski DA, Schultz EA - Asymmetric auxin response precedes asymmetric growth and differentiation of asymmetric leaf1 and asymmetric leaf2 Arabidopsis leaves

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Gene expression

it has been reported that rosette leaves of transgenic plants carrying the ABI4::GUS construct have no detectable GUS activity (Arroyo et al. 2003

Kozuka T, Horiguchi G, Kim GT, Ohgishi M, Sakai T, Tsukaya H - The different growth responses of the Arabidopsis thaliana leaf blade and the petiole during shade avoidance are regulated by photoreceptors and sugar

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Gene expression

In seedlings overexpressing CYP72C1, the expression levels of TCH4 ... were reduced

Takahashi N, Nakazawa M, Shibata K, Yokota T, Ishikawa A, Suzuki K, Kawashima M, Ichikawa T, Shimada H, Matsui M - shk1-D, a dwarf Arabidopsis mutant caused by activation of the CYP72C1 gene, has altered brassinosteroid levels

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Gene expression

The CYP72C1 gene was expressed more strongly in the dark than in the light

Takahashi N, Nakazawa M, Shibata K, Yokota T, Ishikawa A, Suzuki K, Kawashima M, Ichikawa T, Shimada H, Matsui M - shk1-D, a dwarf Arabidopsis mutant caused by activation of the CYP72C1 gene, has altered brassinosteroid levels

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Gene expression

To confirm the expression of CYP72C1, we performed RT-PCR. We prepared total RNA from flowers, siliques, stems, cauline leaves and rosette leaves of 4-week-old plants and from roots in liquid culture. We observed CYP72C1 expression in all tissues examined and relatively strong expression in siliques and roots (Figure 5j)

Takahashi N, Nakazawa M, Shibata K, Yokota T, Ishikawa A, Suzuki K, Kawashima M, Ichikawa T, Shimada H, Matsui M - shk1-D, a dwarf Arabidopsis mutant caused by activation of the CYP72C1 gene, has altered brassinosteroid levels

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Gene expression

In seedlings overexpressing CYP72C1, the expression levels of ... BAS1 were reduced

Takahashi N, Nakazawa M, Shibata K, Yokota T, Ishikawa A, Suzuki K, Kawashima M, Ichikawa T, Shimada H, Matsui M - shk1-D, a dwarf Arabidopsis mutant caused by activation of the CYP72C1 gene, has altered brassinosteroid levels

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Gene expression

In seedlings overexpressing CYP72C1 ... CPD expression was slightly increased

Takahashi N, Nakazawa M, Shibata K, Yokota T, Ishikawa A, Suzuki K, Kawashima M, Ichikawa T, Shimada H, Matsui M - shk1-D, a dwarf Arabidopsis mutant caused by activation of the CYP72C1 gene, has altered brassinosteroid levels

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Gene expression

transgenic plants containing the 2.0-kb upstream promoter region of the CYP72C1 gene fused to the β-glucuronidase gene (GUS ... in light-grown seedlings it was seen in cotyledons

Takahashi N, Nakazawa M, Shibata K, Yokota T, Ishikawa A, Suzuki K, Kawashima M, Ichikawa T, Shimada H, Matsui M - shk1-D, a dwarf Arabidopsis mutant caused by activation of the CYP72C1 gene, has altered brassinosteroid levels

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Gene expression

In bop1-1 plants, KNAT1::GUS was expressed in a pattern similar to that in wild-type plants, but in more expanded regions

Norberg M, Holmlund M, Nilsson O - The BLADE ON PETIOLE genes act redundantly to control the growth and development of lateral organs

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Gene expression

Examination of the expression pattern in single leaves of bop1-1 showed that, unlike in wild-type leaves, KNAT1::GUS was detected in the petioles of cotyledons and rosette leaves

Norberg M, Holmlund M, Nilsson O - The BLADE ON PETIOLE genes act redundantly to control the growth and development of lateral organs

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Gene expression

KNAT6 transcript ... levels ... in the cotyledons and rosette leaves of bop1-1 seedlings were significantly higher than those in wild-type seedlings

Norberg M, Holmlund M, Nilsson O - The BLADE ON PETIOLE genes act redundantly to control the growth and development of lateral organs

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Gene expression

in the case of the KNAT2 that showed only a slightly increased expression in the mutant rosette leaf

Norberg M, Holmlund M, Nilsson O - The BLADE ON PETIOLE genes act redundantly to control the growth and development of lateral organs

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Gene expression

the KNAT1 ... transcript ... levels ... in the cotyledons and rosette leaves of bop1-1 seedlings were significantly higher than those in wild-type seedlings

Norberg M, Holmlund M, Nilsson O - The BLADE ON PETIOLE genes act redundantly to control the growth and development of lateral organs

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Gene expression

the KNAT1 ... there were no notable differences between the wild-type and mutant SAM regions in the expression of these genes

Norberg M, Holmlund M, Nilsson O - The BLADE ON PETIOLE genes act redundantly to control the growth and development of lateral organs

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Gene expression

KNAT2 ... there were no notable differences between the wild-type and mutant SAM regions in the expression of these genes

Norberg M, Holmlund M, Nilsson O - The BLADE ON PETIOLE genes act redundantly to control the growth and development of lateral organs

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Gene expression

KNAT6 ... there were no notable differences between the wild-type and mutant SAM regions in the expression of these genes

Norberg M, Holmlund M, Nilsson O - The BLADE ON PETIOLE genes act redundantly to control the growth and development of lateral organs

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Gene expression

To determine the subcellular localization of the BOP2 protein, a 35S:BOP2�green fluorescent protein (GFP) transgene was introduced into bop1 bop2 plants by Agrobacterium-mediated transformation. Partial complementation of the phenotype conferred by bop1 bop2 was obtained for many of the resulting transgenic plant lines, indicating that the fusion protein had biological activity (data not shown). Confocal laser scanning of a seedling at 4 d after germination shows that in root cells, the BOP2-GFP fusion protein was localized to both the cytoplasm and the nucleus

Hepworth SR, Zhang Y, McKim S, Li X, Haughn GW - BLADE-ON-PETIOLE-dependent signaling controls leaf and floral patterning in Arabidopsis

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Gene expression

BOP2 ... transcripts ... were detected in leaves, stems, apices, flowers, and roots but not in siliques

Hepworth SR, Zhang Y, McKim S, Li X, Haughn GW - BLADE-ON-PETIOLE-dependent signaling controls leaf and floral patterning in Arabidopsis

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Gene expression

To determine the subcellular localization of the BOP2 protein, a 35S:BOP2�green fluorescent protein (GFP) transgene was introduced into bop1 bop2 plants by Agrobacterium-mediated transformation. Partial complementation of the phenotype conferred by bop1 bop2 was obtained for many of the resulting transgenic plant lines, indicating that the fusion protein had biological activity (data not shown). Confocal laser scanning of a seedling at 4 d after germination shows that in root cells, the BOP2-GFP fusion protein was localized to both the cytoplasm and the nucleus

Hepworth SR, Zhang Y, McKim S, Li X, Haughn GW - BLADE-ON-PETIOLE-dependent signaling controls leaf and floral patterning in Arabidopsis

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Gene expression

BOP1 ... transcripts ... were detected in leaves, stems, apices, flowers, and roots but not in siliques

Hepworth SR, Zhang Y, McKim S, Li X, Haughn GW - BLADE-ON-PETIOLE-dependent signaling controls leaf and floral patterning in Arabidopsis

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Gene expression

The expression of the ... ELO3 genes was monitored in different plant organs by RT-PCR: roots, young first leaves, whole seedlings (14 DAG), cotyledons, expanded first leaves, floral buds, and shoot apices (27 DAG). The three ELO genes were expressed in every plant organ examined

Nelissen H, Fleury D, Bruno L, Robles P, De Veylder L, Traas J, Micol JL, Van Montagu M, Inzé D, Van Lijsebettens M - The elongata mutants identify a functional Elongator complex in plants with a role in cell proliferation during organ growth

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Gene expression

The microarray experiment using mRNA of the shoot apices of the elo mutants showed that ... no difference in expression level was seen for the ELO3 gene between the elo3 mutant and Ler

Nelissen H, Fleury D, Bruno L, Robles P, De Veylder L, Traas J, Micol JL, Van Montagu M, Inzé D, Van Lijsebettens M - The elongata mutants identify a functional Elongator complex in plants with a role in cell proliferation during organ growth

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Gene expression

The expression of the ... ELO2 ... was monitored in different plant organs by RT-PCR: roots, young first leaves, whole seedlings (14 DAG), cotyledons, expanded first leaves, floral buds, and shoot apices (27 DAG ... were expressed in every plant organ examined

Nelissen H, Fleury D, Bruno L, Robles P, De Veylder L, Traas J, Micol JL, Van Montagu M, Inzé D, Van Lijsebettens M - The elongata mutants identify a functional Elongator complex in plants with a role in cell proliferation during organ growth

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Gene expression

The microarray experiment using mRNA of the shoot apices of the elo mutants showed that the expression of ELO2 in elo2 was only 20.1% that of the wild type

Nelissen H, Fleury D, Bruno L, Robles P, De Veylder L, Traas J, Micol JL, Van Montagu M, Inzé D, Van Lijsebettens M - The elongata mutants identify a functional Elongator complex in plants with a role in cell proliferation during organ growth

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Gene expression

The expression of the ELO1 ... was monitored in different plant organs by RT-PCR: roots, young first leaves, whole seedlings (14 DAG), cotyledons, expanded first leaves, floral buds, and shoot apices (27 DAG ... were expressed in every plant organ examined ... in every

Nelissen H, Fleury D, Bruno L, Robles P, De Veylder L, Traas J, Micol JL, Van Montagu M, Inzé D, Van Lijsebettens M - The elongata mutants identify a functional Elongator complex in plants with a role in cell proliferation during organ growth

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Gene expression

The expression of the ACL5 gene was examined in wild-type and tkv plants by reverse transcription (RT)-PCR and was found to be similarly up-regulated in tkv mutant plants (Fig. 7

Clay NK, Nelson T - Arabidopsis thickvein mutation affects vein thickness and organ vascularization, and resides in a provascular cell-specific spermine synthase involved in vein definition and in polar auxin transport

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Gene expression

The procambial-specific expression pattern continues during primary root development (Figs. 8, E and G, 9I) and early leaf development after an initially broad expression domain in leaf primordia (Figs. 8C and 9, E–H

Clay NK, Nelson T - Arabidopsis thickvein mutation affects vein thickness and organ vascularization, and resides in a provascular cell-specific spermine synthase involved in vein definition and in polar auxin transport

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Gene expression

The procambial-specific expression pattern continues during primary root development (Figs. 8, E and G, 9I) and early leaf development after an initially broad expression domain in leaf primordia (Figs. 8C and 9, E–H

Clay NK, Nelson T - Arabidopsis thickvein mutation affects vein thickness and organ vascularization, and resides in a provascular cell-specific spermine synthase involved in vein definition and in polar auxin transport

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Gene expression

CYCD3;1 expressions were examined by RNA gel blot analysis and were found to be unchanged in smpepi ... plants

Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf

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Gene expression

SWP expression level was examined by RNA gel blot analysis and RT-PCR and was found to be ... normal in ... smp2-1

Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf

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Gene expression

ANT overexpression activated ectopic expression of CYCD3;1

Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf

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Gene expression

RT-PCR results indicated that the transcripts of ... SMP1 ... were reduced in smp1epi homozygous plants

Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf

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Gene expression

full-length SWP cDNA driven by the 35S CaMV promoter was introduced into smpepi mutant plants ... RT-PCR results confirmed that two of these restored lines expressed a wild-type level of fully spliced SWP transcript and more so than the line that exhibited the mutant phenotype

Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf

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Gene expression

transgenic copy of the SMP1 promoter driving the β-glucuronidase (GUS) reporter gene ... After germination, SMP1 mRNA expression was ... not in the SAM

Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf

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Gene expression

transgenic copy of the SMP1 promoter driving the β-glucuronidase (GUS) reporter gene ... SMP1 promoter activity was also found throughout very young leaf primordia (Figure 8J) and as the leaf matured, was turned off in a tip-to-base manner (Figure 8K). This pattern of GUS expression overlapped with the general pattern of cell proliferation and presaged the basipetal wave of cell maturation that would flow from leaf tip to leaf base

Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf

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Gene expression

transgenic copy of the SMP1 promoter driving the β-glucuronidase (GUS) reporter gene ... After germination, SMP1 mRNA expression was found throughout leaf primordia

Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf

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Gene expression

SMP1 promoter activity was also found throughout very young leaf primordia

Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf

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Gene expression

CDC2A expression ... was ... unchanged in ... SMP1-overexpressing plants

Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf

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Gene expression

CDC2A expression also was found to be unchanged in ... SMP1-overexpressing plants

Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf

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Gene expression

full-length SWP cDNA driven by the 35S CaMV promoter was introduced into smpepi mutant plants. Because of the moderate infertility of these plants, only four independent lines were obtained ... transcript levels of ... SMP2 were also increased in all three lines

Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf

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Gene expression

SMP1 mRNA expression was found throughout early globular-staged embryos

Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf

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Gene expression

SWP expression level was examined by RNA gel blot analysis and RT-PCR and was found to be undetectable in smpepi plants

Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf

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Gene expression

CYCD3;1 expressions were examined by RNA gel blot analysis and were found to be unchanged in ... SMP1-overexpressing plants

Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf

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Gene expression

CDC2A expression also was found to be unchanged in smpepi ... plants

Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf

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Gene expression

full-length SWP cDNA driven by the 35S CaMV promoter was introduced into smpepi mutant plants. Because of the moderate infertility of these plants, only four independent lines were obtained ... transcript levels of SMP1 ... were also increased in all three lines

Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf

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Gene expression

ANT ... expressions were examined by RNA gel blot analysis and were found to be unchanged in ... SMP1-overexpressing plants

Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf

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Gene expression

SWP expression level was examined by RNA gel blot analysis and RT-PCR and was found to be ... normal ... in smp1-1

Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf

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Gene expression

transgenic copy of the SMP1 promoter driving the β-glucuronidase (GUS) reporter gene ... bent cotyledon stage where SMP1 promoter activity increased in procambial cells

Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf

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Gene expression

SWP expression level was examined by RNA gel blot analysis and RT-PCR and was found to be ... normal in SMP1-overexpressing plants

Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf

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Gene expression

ANT ... expressions were examined by RNA gel blot analysis and were found to be unchanged in smpepi ... plants

Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf

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Gene expression

CDC2A expression ... was ... unchanged in smpepi ... plants

Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf

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Gene expression

SMP1 mRNA ... expression intensified in the cotyledon primordia of heart-staged embryos

Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf

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Gene expression

RT-PCR results indicated that the transcripts of ... SMP2 were reduced in smp1epi homozygous plants

Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf

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Gene expression

SMP1 expression is associated with regions of cell proliferation in lateral organs

Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf

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Gene expression

We also generated transgenic plants expressing ProARF2:GUS in order to monitor ARF2 expression during development. GUS activity was detected in the peripheral zone of cotyledons ... in 3-day-old, light-grown transgenic seedlings

Okushima Y, Mitina I, Quach HL, Theologis A - AUXIN RESPONSE FACTOR 2 (ARF2): a pleiotropic developmental regulator

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Gene expression

The results shown in Figure 1(c) indicate that ARF2 expression in wild-type seedlings ... slightly induced by IAA treatment

Okushima Y, Mitina I, Quach HL, Theologis A - AUXIN RESPONSE FACTOR 2 (ARF2): a pleiotropic developmental regulator

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Gene expression

We also generated transgenic plants expressing ProARF2:GUS in order to monitor ARF2 expression during development ... little or no GUS expression was detected in cotyledons of etiolated seedlings

Okushima Y, Mitina I, Quach HL, Theologis A - AUXIN RESPONSE FACTOR 2 (ARF2): a pleiotropic developmental regulator

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Gene expression

Previous studies by Ulmasov et al. (1999a) have shown that ARF2 is expressed in all major plant organs including roots, rosette and caulin leaves, flowers and siliques. These expression characteristics were also confirmed by us using RT-PCR analysis (data not shown

Okushima Y, Mitina I, Quach HL, Theologis A - AUXIN RESPONSE FACTOR 2 (ARF2): a pleiotropic developmental regulator

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Gene expression

AN3 ... expressed at detectable levels in the shoot tip

Horiguchi G, Kim GT, Tsukaya H - The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana

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Gene expression

The expression domains of AtGRF4 ... partially overlapped with the AN3 expression domain, mainly near the midvein

Horiguchi G, Kim GT, Tsukaya H - The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana

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Gene expression

AtGRF5 ... undetectable in mature leaves

Horiguchi G, Kim GT, Tsukaya H - The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana

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Gene expression

GUS activities in ... AtGRF9 reporter plants were clearly restricted to the lower half of the leaf primordium ... Consistent with this analysis the RT-PCR analysis showed that AN3, AtGRF5, and AtGRF9 are expressed at stronger levels in the lower half than in the upper half of the primordium

Horiguchi G, Kim GT, Tsukaya H - The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana

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Gene expression

AN3 expression was undetectable in mature leaves

Horiguchi G, Kim GT, Tsukaya H - The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana

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Gene expression

AN3 was strongly expressed in the basal region of leaf primordia of 5-day-old plants

Horiguchi G, Kim GT, Tsukaya H - The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana

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Gene expression

AtGRF9 ... undetectable in mature leaves

Horiguchi G, Kim GT, Tsukaya H - The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana

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Gene expression

GUS activities in ... AtGRF5 ... reporter plants ... were clearly restricted to the lower half of the leaf primordium ... Consistent with this analysis the RT-PCR analysis showed that AN3, AtGRF5, and AtGRF9 are expressed at stronger levels in the lower half than in the upper half of the primordium

Horiguchi G, Kim GT, Tsukaya H - The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana

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Gene expression

The expression domains of ... AtGRF6 partially overlapped with the AN3 expression domain, mainly near the midvein

Horiguchi G, Kim GT, Tsukaya H - The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana

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Gene expression

AtGRF9 ... expressed uniformly in the primordium

Horiguchi G, Kim GT, Tsukaya H - The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana

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Gene expression

AtGRF7 was strongly expressed in cells surrounding the vasculature

Horiguchi G, Kim GT, Tsukaya H - The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana

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Gene expression

GUS activities in AN3 ... reporter plants ... were clearly restricted to the lower half of the leaf primordium ... Consistent with this analysis the RT-PCR analysis showed that AN3, AtGRF5, and AtGRF9 are expressed at stronger levels in the lower half than in the upper half of the primordium

Horiguchi G, Kim GT, Tsukaya H - The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana

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Gene expression

AN3 was ... expressed ... weakly in the shoot apical meristem

Horiguchi G, Kim GT, Tsukaya H - The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana

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Gene expression

AtGRF5 ... expressed uniformly in the primordium

Horiguchi G, Kim GT, Tsukaya H - The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana

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Gene expression

In spite of the striking similarity of the leaf phenotypes of as and aba1 mutants, ectopic expression of the KNAT1 ... was not found, as tested by RT-PCR amplifications of RNA extracted from the leaves of aba1/aba1 plants (data not shown

Barrero JM, Piqueras P, González-Guzmán M, Serrano R, Rodríguez PL, Ponce MR, Micol JL - A mutational analysis of the ABA1 gene of Arabidopsis thaliana highlights the involvement of ABA in vegetative development

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Gene expression

In spite of the striking similarity of the leaf phenotypes of as and aba1 mutants, ectopic expression of ... STM ... was not found, as tested by RT-PCR amplifications of RNA extracted from the leaves of aba1/aba1 plants (data not shown

Barrero JM, Piqueras P, González-Guzmán M, Serrano R, Rodríguez PL, Ponce MR, Micol JL - A mutational analysis of the ABA1 gene of Arabidopsis thaliana highlights the involvement of ABA in vegetative development

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Gene expression

In spite of the striking similarity of the leaf phenotypes of as and aba1 mutants, ectopic expression of the ... KNAT2 ... was not found, as tested by RT-PCR amplifications of RNA extracted from the leaves of aba1/aba1 plants (data not shown

Barrero JM, Piqueras P, González-Guzmán M, Serrano R, Rodríguez PL, Ponce MR, Micol JL - A mutational analysis of the ABA1 gene of Arabidopsis thaliana highlights the involvement of ABA in vegetative development

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Gene expression

Our examination of ANT expression by real time RT-PCR produced results that are in agreement with previous reports of ANT expression in ... seedlings

Nole-Wilson S, Tranby TL, Krizek BA - AINTEGUMENTA-like (AIL) genes are expressed in young tissues and may specify meristematic or division-competent states

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Gene expression

Our examination of ANT expression by real time RT-PCR ... we also observed low expression in mature rosette leaves, which was not previously detected by RNA gel blot analysis (Klucher et al., 1996

Nole-Wilson S, Tranby TL, Krizek BA - AINTEGUMENTA-like (AIL) genes are expressed in young tissues and may specify meristematic or division-competent states

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Gene expression

ANT is expressed at higher levels in most tissues compared to other members of the AIL group

Nole-Wilson S, Tranby TL, Krizek BA - AINTEGUMENTA-like (AIL) genes are expressed in young tissues and may specify meristematic or division-competent states

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Gene expression

the gene encoding ribosomal protein L10A (At2g27530) ... were induced in the ATAF2 KO line compared to the wild type in the non-wounded case only

Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis

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Gene expression

PDF1.1 ... were induced in the KO, in both non-wounded and wounded (30 min) conditions

Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis

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Gene expression

Arabidopsis plants transformed with the ATAF2 promoter fused to the GUS reporter gene (PATAF2:GUS) confirmed the sqRT-PCR results, indicating that ATAF2 was expressed at varying levels in all plant tissues. ATAF2 was highly expressed in ... leaves

Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis

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Gene expression

The expression pattern in cotyledons and leaves was not homogeneous, but GUS staining was present only in particular cell types (Figure 3b). Microscopic examination indicated that ATAF2 was expressed mainly in cells surrounding the sub-stomatal cavity

Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis

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Gene expression

When plants were placed into a high osmotic medium containing mannitol to induce water loss, ATAF2 was induced 2.6-fold

Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis

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Gene expression

Wound induction of ATAF2 was not blocked by mutations affecting ABA biosynthesis (aba1; Koornneef et al., 1982)

Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis

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Gene expression

GST1 (At1g02930) ... were induced in the ATAF2 KO line compared to the wild type in the non-wounded case only

Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis

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Gene expression

ATAF2 ... In mature leaves, strong expression was observed in the hydathodes

Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis

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Gene expression

To confirm the GUS staining results, we measured the expression level of ATAF2 at the wound site (local), in the non-wounded tissue next to the wound site (adjacent), in non-wounded leaves of a wounded plant (systemic) and in leaves of a control non-wounded plant (NWP) using sqRT-PCR (Figure 4). ATAF2 was induced after 30 min in the local tissue (approximately 13× over leaves of non-wounded plants), to a lower extent in the adjacent tissue (approximately 4×) and less in systemic leaves (approximately 2×). This high level of wounding induction is transient, and 5 h after wounding, induction levels were markedly reduced (6×, 2× and 2× over leaves of non-wounded plants, respectively

Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis

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Gene expression

Wound induction of ATAF2 was not blocked by mutations affecting ... ABA response pathways ... abi2

Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis

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Gene expression

PATAF2:GUS transgenic Arabidopsis plants showed a strong induction of GUS activity immediately around the damaged area of a wounded leaf

Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis

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Gene expression

ATAF2 expression was induced in leaves by the application of ... SA (6.5X)

Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis

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Gene expression

PR1 (At2g14610) ... were induced in the ATAF2 KO line compared to the wild type in the non-wounded case only

Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis

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Gene expression

Arabidopsis plants transformed with the ATAF2 promoter fused to the GUS reporter gene (PATAF2:GUS) confirmed the sqRT-PCR results, indicating that ATAF2 was expressed at varying levels in all plant tissues. ATAF2 was highly expressed in cotyledons

Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis

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Gene expression

However, plants kept in the dark for 5 h showed increased ATAF2 expression (2.1-fold; Figure 5a), indicating that light negatively influences ATAF2 expression

Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis

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Gene expression

ATAF2 expression ... in leaves ... ABA treatment did not have any effect

Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis

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Gene expression

When the response of ATAF2 to sugar was tested by transferring plants to liquid MS medium in the presence or absence of 3% sucrose 4 days prior to hormone or stress treatments, ATAF2 expression was higher in all treatments in the absence of sugars. This was especially evident in the response to dark, where ATAF2 induction was 2.6 times higher in the absence than in the presence of sucrose

Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis

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Gene expression

ATAF2 expression was induced in leaves by the application of MeJA (7.4X)

Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis

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Gene expression

ATAF2 ... in older senescing cotyledons, expression is clearly restricted to stomatal guard cells

Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis

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Gene expression

the H-protein promoter binding factor-2a (At3g47500) were induced in the ATAF2 KO line compared to the wild type in the non-wounded case only

Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis

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Gene expression

Expression analysis using semi-quantitative real-time reverse transcription polymerase chain reaction (sqRT-PCR) indicated that ATAF2 was expressed in roots, leaves, seedlings and mature flowers (Figure 2). The expression was highest in roots and leaves, and lowest in flower buds and bolt stems

Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis

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Gene expression

a gene encoding a 33 kDa secretory protein-related protein (At5g48540) were induced in the KO, in both non-wounded and wounded (30 min) conditions

Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis

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Gene expression

PDF1.2 ... were induced in the KO, in both non-wounded and wounded (30 min) conditions

Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis

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Gene expression

ATAF2 ... In mature leaves ... a low background level of expression was occasionally observed in the whole leaf and/or in the vascular tissue

Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis

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Gene expression

Wound induction of ATAF2 was not blocked by mutations affecting ... ABA response pathways ... abi1

Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis

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Gene expression

We then compared expression of AP1, FUL, and SEP3 in mature rosette leaves of wild-type plants or those overexpressing FT (Figure 2B) or TFT (see Supplemental Figure 3B online). Plants were grown for 30 d under conditions that enhance curling (conditions A2 and A4 in Table 1, respectively). All three genes were highly misexpressed in the rosette leaves of both transgenic lines. Fold induction ranged between 5.4 (FUL) and 21 (SEP3) for Pro35S:FT plants grown under long days

Teper-Bamnolker P, Samach A - The flowering integrator FT regulates SEPALLATA3 and FRUITFULL accumulation in Arabidopsis leaves

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Gene expression

In 30-d-old rosette leaves, we detected a consistent 10-fold decrease in the expression of SEP3 and FUL in ft-2 mutants

Teper-Bamnolker P, Samach A - The flowering integrator FT regulates SEPALLATA3 and FRUITFULL accumulation in Arabidopsis leaves

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Gene expression

We therefore asked whether FD is required for FT-dependent accumulation of SEP3 and FUL in mature rosette leaves. Indeed, the fd-1 mutation had an effect similar to that of ft-2 in reducing the expression of these genes in mature rosette leaves (Figure 2C

Teper-Bamnolker P, Samach A - The flowering integrator FT regulates SEPALLATA3 and FRUITFULL accumulation in Arabidopsis leaves

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Gene expression

We then compared expression of AP1, FUL, and SEP3 in mature rosette leaves of wild-type plants or those overexpressing FT (Figure 2B) or TFT (see Supplemental Figure 3B online). Plants were grown for 30 d under conditions that enhance curling (conditions A2 and A4 in Table 1, respectively). All three genes were highly misexpressed in the rosette leaves of both transgenic lines. Fold induction ranged between 5.4 (FUL) and 21 (SEP3) for Pro35S:FT plants grown under long days

Teper-Bamnolker P, Samach A - The flowering integrator FT regulates SEPALLATA3 and FRUITFULL accumulation in Arabidopsis leaves

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Gene expression

In 30-d-old rosette leaves, we detected a consistent 10-fold decrease in the expression of SEP3 and FUL in ft-2 mutants

Teper-Bamnolker P, Samach A - The flowering integrator FT regulates SEPALLATA3 and FRUITFULL accumulation in Arabidopsis leaves

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Gene expression

We then compared expression of AP1, FUL, and SEP3 in mature rosette leaves of wild-type plants or those overexpressing FT (Figure 2B) or TFT (see Supplemental Figure 3B online). Plants were grown for 30 d under conditions that enhance curling (conditions A2 and A4 in Table 1, respectively). All three genes were highly misexpressed in the rosette leaves of both transgenic lines. Fold induction ranged between 5.4 (FUL) and 21 (SEP3) for Pro35S:FT plants grown under long days

Teper-Bamnolker P, Samach A - The flowering integrator FT regulates SEPALLATA3 and FRUITFULL accumulation in Arabidopsis leaves

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Gene expression

We therefore asked whether FD is required for FT-dependent accumulation of SEP3 and FUL in mature rosette leaves. Indeed, the fd-1 mutation had an effect similar to that of ft-2 in reducing the expression of these genes in mature rosette leaves (Figure 2C

Teper-Bamnolker P, Samach A - The flowering integrator FT regulates SEPALLATA3 and FRUITFULL accumulation in Arabidopsis leaves

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Gene expression

The expression of AS2 was not altered in the ... GTE6 RNAi plants

Chua YL, Channelière S, Mott E, Gray JC - The bromodomain protein GTE6 controls leaf development in Arabidopsis by histone acetylation at ASYMMETRIC LEAVES1

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Gene expression

The expression of AS2 was not altered in the GTE6 overexpressing mutants

Chua YL, Channelière S, Mott E, Gray JC - The bromodomain protein GTE6 controls leaf development in Arabidopsis by histone acetylation at ASYMMETRIC LEAVES1

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Gene expression

The expression of other genes involved in leaf development, such as ... TPC2 ... was also investigated. However, no change in the transcript levels of these genes was observed in the GTE6 mutants, suggesting that GTE6 is not regulating the expression of these genes

Chua YL, Channelière S, Mott E, Gray JC - The bromodomain protein GTE6 controls leaf development in Arabidopsis by histone acetylation at ASYMMETRIC LEAVES1

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Gene expression

The expression of other genes involved in leaf development, such as ... CLF ... was also investigated. However, no change in the transcript levels of these genes was observed in the GTE6 mutants, suggesting that GTE6 is not regulating the expression of these genes

Chua YL, Channelière S, Mott E, Gray JC - The bromodomain protein GTE6 controls leaf development in Arabidopsis by histone acetylation at ASYMMETRIC LEAVES1

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Gene expression

Elevated GTE6 expression in the 35S::GTE6 plants resulted in more AS1 transcripts

Chua YL, Channelière S, Mott E, Gray JC - The bromodomain protein GTE6 controls leaf development in Arabidopsis by histone acetylation at ASYMMETRIC LEAVES1

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Gene expression

The expression of other genes involved in leaf development, such as ... DRL ... was also investigated. However, no change in the transcript levels of these genes was observed in the GTE6 mutants, suggesting that GTE6 is not regulating the expression of these genes

Chua YL, Channelière S, Mott E, Gray JC - The bromodomain protein GTE6 controls leaf development in Arabidopsis by histone acetylation at ASYMMETRIC LEAVES1

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Gene expression

In the basal rosette leaves of 21-d-old wild-type plants, GTE6 transcripts are more abundant in leaves 6 and 7, which possess narrow elliptical laminae, than in leaves 1-4, which have round laminae (Fig. 1C,D), suggesting a possible correlation between GTE6 expression and the formation of elliptical leaf laminae in mature leaves

Chua YL, Channelière S, Mott E, Gray JC - The bromodomain protein GTE6 controls leaf development in Arabidopsis by histone acetylation at ASYMMETRIC LEAVES1

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Gene expression

The expression of other genes involved in leaf development, such as ... ROT3 ... was also investigated. However, no change in the transcript levels of these genes was observed in the GTE6 mutants, suggesting that GTE6 is not regulating the expression of these genes

Chua YL, Channelière S, Mott E, Gray JC - The bromodomain protein GTE6 controls leaf development in Arabidopsis by histone acetylation at ASYMMETRIC LEAVES1

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Gene expression

The T-DNA insertions resulted in fewer GTE6 transcripts in leaf 7 (Fig. 2B), suggesting misregulation of GTE6 expression. The transcript abundance of GTE6 in leaf 7 of the insertion mutants was similar to leaf 4 of the wild-type and the insertion mutant plants, suggesting that the T-DNA insertions have disrupted the activation of GTE6 expression in the mature leaves ... Thus, elevated expression of GTE6 in leaves 6 and 7 of wild-type plants is important for the development of the elliptical leaf lamina

Chua YL, Channelière S, Mott E, Gray JC - The bromodomain protein GTE6 controls leaf development in Arabidopsis by histone acetylation at ASYMMETRIC LEAVES1

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Gene expression

The expression of other genes involved in leaf development, such as AN ... was also investigated. However, no change in the transcript levels of these genes was observed in the GTE6 mutants, suggesting that GTE6 is not regulating the expression of these genes

Chua YL, Channelière S, Mott E, Gray JC - The bromodomain protein GTE6 controls leaf development in Arabidopsis by histone acetylation at ASYMMETRIC LEAVES1

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Gene expression

decreased expression of GTE6 in the RNAi plants resulted in fewer AS1 transcripts

Chua YL, Channelière S, Mott E, Gray JC - The bromodomain protein GTE6 controls leaf development in Arabidopsis by histone acetylation at ASYMMETRIC LEAVES1

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Gene expression

In wild-type plants, PIAA3::GUS was expressed in ... cotyledons

Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana

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Gene expression

ARF19 ... induced in senescing leaves (Lin and Wu, 2004) and this was confirmed by RNA gel blot analysis (Fig. 8C

Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana

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Gene expression

Ectopic PIAA3::GUS ... stain was also observed in the leaves of arf1-4 seedlings

Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana

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Gene expression

IAA5 mRNA levels were increased in auxin-treated arf1-4 seedlings

Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana

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Gene expression

RNA levels of ... IAA5 ... in arf2 flowers (Fig. 6S) and seedlings (data not shown) were similar to those of Columbia plants

Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana

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Gene expression

IAA2 ... mRNA levels were increased in auxin-treated arf1-4 seedlings

Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana

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Gene expression

In wild-type plants ... PIAA7::GUS was expressed in ... shoot meristems

Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana

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Gene expression

PARF1::GUS ... X-Gluc staining appeared throughout 8-day-old seedlings and in rosette leaves (data not shown

Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana

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Gene expression

Microarray studies also indicated that ARF2 mRNA increased during senescence (Lin and Wu, 2004). We examined PARF2::GUS::ARF2 plants (Li et al., 2004) to determine the levels of ARF2 protein in senescing leaves. In mature, fresh fully expanded rosette leaves or in those placed in the dark for 2 days, little GUS staining was observed (Fig. 4A,B). After 4 and 6 days in darkness, GUS staining was evident (Fig. 4C,D). GUS activity was highest in vasculature, but was also present in ground tissue. Together these data indicate that ARF2 accumulated during leaf senescence

Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana

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Gene expression

RNA gel blot hybridizations confirmed that endogenous ... IAA7 mRNA levels were increased in arf1-4 flowers and seedlings, and in arf1-4 arf2-8 flowers (Fig. 6S

Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana

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Gene expression

Ectopic ... PIAA7::GUS stain was also observed in the leaves of arf1-4 seedlings

Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana

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Gene expression

ARF1 mRNA decreased as senescence progressed in ... Columbia ... plants

Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana

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Gene expression

ARF1 mRNA decreased as senescence progressed in ... arf2-8 plants

Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana

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Gene expression

In wild-type plants, PIAA3::GUS was expressed in ... expanding leaves

Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana

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Gene expression

PARF2::GUS ... X-Gluc staining appeared throughout 8-day-old seedlings and in rosette leaves (data not shown

Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana

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Gene expression

RNA levels of ... IAA7 ... in arf2 flowers (Fig. 6S) and seedlings (data not shown) were similar to those of Columbia plants

Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana

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Gene expression

NPH4/ARF7 mRNA levels in arf2-8 leaves were approximately twofold higher than in Columbia leaves at the start of the experiment

Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana

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Gene expression

RNA gel blot hybridizations confirmed that endogenous IAA3 ... mRNA levels were increased in arf1-4 flowers and seedlings, and in arf1-4 arf2-8 flowers (Fig. 6S

Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana

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Gene expression

RNA levels of IAA2 ... in arf2 flowers (Fig. 6S) and seedlings (data not shown) were similar to those of Columbia plants

Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana

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Gene expression

ARF1 ... present in roots, rosette leaves, cauline leaves and flowers (Ulmasov et al., 1999b

Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana

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Gene expression

Younger, expanding leaves of PARF2::GUS::ARF2 plants also stained in the vasculature and ground tissue, both in freshly detached leaves and in those placed in the dark for 4 days

Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana

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Gene expression

ARF2 mRNA ... present in roots, rosette leaves, cauline leaves and flowers (Ulmasov et al., 1999b

Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana

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Gene expression

RNA levels of ... IAA3 ... in arf2 flowers (Fig. 6S) and seedlings (data not shown) were similar to those of Columbia plants

Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana

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Gene expression

NPH4/ARF7 ... induced in senescing leaves (Lin and Wu, 2004) and this was confirmed by RNA gel blot analysis (Fig. 8C

Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana

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Gene expression

Younger, expanding leaves of PARF2::GUS::ARF2 plants also stained in the vasculature ... both in freshly detached leaves and in those placed in the dark for 4 days

Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana

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Gene expression

ARF1 mRNA levels were quantified in three independent blots and were found to be slightly higher (2.7±0.9-fold) in freshly detached arf2 leaves when compared with Columbia leaves

Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana

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Gene expression

Zip III gene CORONA (CNA)17 ... shows elevated expression in se-3 plants

Grigg SP, Canales C, Hay A, Tsiantis M - SERRATE coordinates shoot meristem function and leaf axial patterning in Arabidopsis

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Gene expression

Misexpression of PHB in se mutants was evident at the eight-cell stage of embryogenesis

Grigg SP, Canales C, Hay A, Tsiantis M - SERRATE coordinates shoot meristem function and leaf axial patterning in Arabidopsis

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Gene expression

PHB ... in se plants it is expressed broadly, extending into the abaxial ... domain

Grigg SP, Canales C, Hay A, Tsiantis M - SERRATE coordinates shoot meristem function and leaf axial patterning in Arabidopsis

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Gene expression

BP ... are not ... misregulated in se mutants

Grigg SP, Canales C, Hay A, Tsiantis M - SERRATE coordinates shoot meristem function and leaf axial patterning in Arabidopsis

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Gene expression

se-3 plants with hypertrophic apices express PHB transcript strongly ... lateral organ primordia

Grigg SP, Canales C, Hay A, Tsiantis M - SERRATE coordinates shoot meristem function and leaf axial patterning in Arabidopsis

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Gene expression

Levels of miR165 ... primary transcripts were elevated in se mutants compared to wild type

Grigg SP, Canales C, Hay A, Tsiantis M - SERRATE coordinates shoot meristem function and leaf axial patterning in Arabidopsis

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Gene expression

Levels of ... miR166 primary transcripts were elevated in se mutants compared to wild type

Grigg SP, Canales C, Hay A, Tsiantis M - SERRATE coordinates shoot meristem function and leaf axial patterning in Arabidopsis

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Gene expression

KNAT2 are not ... misregulated in se mutants

Grigg SP, Canales C, Hay A, Tsiantis M - SERRATE coordinates shoot meristem function and leaf axial patterning in Arabidopsis

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Gene expression

PHB expression domain is expanded in se mutant SAMs

Grigg SP, Canales C, Hay A, Tsiantis M - SERRATE coordinates shoot meristem function and leaf axial patterning in Arabidopsis

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Gene expression

All three se alleles show increased expression of ... PHB

Grigg SP, Canales C, Hay A, Tsiantis M - SERRATE coordinates shoot meristem function and leaf axial patterning in Arabidopsis

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Gene expression

STM ... are not ... misregulated in se mutants

Grigg SP, Canales C, Hay A, Tsiantis M - SERRATE coordinates shoot meristem function and leaf axial patterning in Arabidopsis

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Gene expression

in arf2 mutants ... we detected ... CYCD3;1 ... expression in mature leaves of mutant but not wild-type plants, indicating that loss of ARF2 function prolongs expression of these genes

Schruff MC, Spielman M, Tiwari S, Adams S, Fenby N, Scott RJ - The AUXIN RESPONSE FACTOR 2 gene of Arabidopsis links auxin signalling, cell division, and the size of seeds and other organs

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Gene expression

in arf2 mutants ... ARGOS was expressed in mature leaf and stem at similar levels in mutants and wild type

Schruff MC, Spielman M, Tiwari S, Adams S, Fenby N, Scott RJ - The AUXIN RESPONSE FACTOR 2 gene of Arabidopsis links auxin signalling, cell division, and the size of seeds and other organs

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Gene expression

RNA gel blot analysis has shown that ARF2 is expressed in roots, leaves, flowers and siliques (Ulmasov et al., 1999b

Schruff MC, Spielman M, Tiwari S, Adams S, Fenby N, Scott RJ - The AUXIN RESPONSE FACTOR 2 gene of Arabidopsis links auxin signalling, cell division, and the size of seeds and other organs

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Gene expression

in arf2 mutants ... we detected both ANT ... expression in mature leaves of mutant but not wild-type plants

Schruff MC, Spielman M, Tiwari S, Adams S, Fenby N, Scott RJ - The AUXIN RESPONSE FACTOR 2 gene of Arabidopsis links auxin signalling, cell division, and the size of seeds and other organs

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Gene expression

We found that hyp7 has similar GL2::GUS expression patterns in leaf trichomes

Sugimoto-Shirasu K, Roberts GR, Stacey NJ, McCann MC, Maxwell A, Roberts K - RHL1 is an essential component of the plant DNA topoisomerase VI complex and is required for ploidy-dependent cell growth

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Gene expression

all topo VI genes, AtSPO11-3/RHL2, AtTOP6B/HYP6/RHL3, and RHL1, are expressed in proliferating and endoreduplicating cells

Sugimoto-Shirasu K, Roberts GR, Stacey NJ, McCann MC, Maxwell A, Roberts K - RHL1 is an essential component of the plant DNA topoisomerase VI complex and is required for ploidy-dependent cell growth

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Gene expression

we found that the topo II gene, AtTOP2, is preferentially expressed in proliferating cells

Sugimoto-Shirasu K, Roberts GR, Stacey NJ, McCann MC, Maxwell A, Roberts K - RHL1 is an essential component of the plant DNA topoisomerase VI complex and is required for ploidy-dependent cell growth

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Gene expression

all topo VI genes, AtSPO11-3/RHL2, AtTOP6B/HYP6/RHL3, and RHL1, are expressed in proliferating and endoreduplicating cells

Sugimoto-Shirasu K, Roberts GR, Stacey NJ, McCann MC, Maxwell A, Roberts K - RHL1 is an essential component of the plant DNA topoisomerase VI complex and is required for ploidy-dependent cell growth

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Gene expression

in vivo visualization revealed that the AtSPO11–3/RHL2 protein fused to GFP is present in ... shoot apical meristem

Sugimoto-Shirasu K, Roberts GR, Stacey NJ, McCann MC, Maxwell A, Roberts K - RHL1 is an essential component of the plant DNA topoisomerase VI complex and is required for ploidy-dependent cell growth

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Gene expression

all topo VI genes, AtSPO11-3/RHL2, AtTOP6B/HYP6/RHL3, and RHL1, are expressed in proliferating and endoreduplicating cells

Sugimoto-Shirasu K, Roberts GR, Stacey NJ, McCann MC, Maxwell A, Roberts K - RHL1 is an essential component of the plant DNA topoisomerase VI complex and is required for ploidy-dependent cell growth

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Gene expression

in vivo visualization revealed that the AtSPO11–3/RHL2 protein fused to GFP is present in ... young leaf petiole cells

Sugimoto-Shirasu K, Roberts GR, Stacey NJ, McCann MC, Maxwell A, Roberts K - RHL1 is an essential component of the plant DNA topoisomerase VI complex and is required for ploidy-dependent cell growth

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Gene expression

dexamethasone (Dex)-inducible system ... Arabidopsis transgenic plants that can express geminivirus RepA protein (RepAwt) in an inducible manner ... we detected the viral protein by immunofluorescence in cross sections of leaves. As shown in Figure 1F, RepA accumulates in all leaf cell layers after Dex treatment

Desvoyes B, Ramirez-Parra E, Xie Q, Chua NH, Gutierrez C - Cell type-specific role of the retinoblastoma/E2F pathway during Arabidopsis leaf development

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Gene expression

dexamethasone (Dex)-inducible system ... Arabidopsis transgenic plants that can express geminivirus RepA protein (RepAwt) in an inducible manner ... PCNA ... expression ... increased in the RepAwt-expressing plants

Desvoyes B, Ramirez-Parra E, Xie Q, Chua NH, Gutierrez C - Cell type-specific role of the retinoblastoma/E2F pathway during Arabidopsis leaf development

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Gene expression

dexamethasone (Dex)-inducible system ... Arabidopsis transgenic plants that can express geminivirus RepA protein (RepAwt) in an inducible manner ... we determined by real-time reverse transcription (RT)-PCR the mRNA levels of ... after induction of the viral protein ... E2Fc ... was up-regulated in these plants

Desvoyes B, Ramirez-Parra E, Xie Q, Chua NH, Gutierrez C - Cell type-specific role of the retinoblastoma/E2F pathway during Arabidopsis leaf development

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Gene expression

dexamethasone (Dex)-inducible system ... Arabidopsis transgenic plants that can express geminivirus RepA protein (RepAwt) in an inducible manner ... CDC6 ... expression ... increased in the RepAwt-expressing plants

Desvoyes B, Ramirez-Parra E, Xie Q, Chua NH, Gutierrez C - Cell type-specific role of the retinoblastoma/E2F pathway during Arabidopsis leaf development

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Gene expression

dexamethasone (Dex)-inducible system ... Arabidopsis transgenic plants that can express geminivirus RepA protein (RepAwt) in an inducible manner ... expressing a translational fusion of the β-glucuronidase (GUS) reporter gene with the destruction box of cyclin B1;1, a useful G2/M marker ... after induction by Dex, we observed a stronger GUS expression in the leaf primordia

Desvoyes B, Ramirez-Parra E, Xie Q, Chua NH, Gutierrez C - Cell type-specific role of the retinoblastoma/E2F pathway during Arabidopsis leaf development

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Gene expression

dexamethasone (Dex)-inducible system ... Arabidopsis transgenic plants that can express geminivirus RepA protein (RepAwt) in an inducible manner ... CDT1 ... expression ... increased in the RepAwt-expressing plants

Desvoyes B, Ramirez-Parra E, Xie Q, Chua NH, Gutierrez C - Cell type-specific role of the retinoblastoma/E2F pathway during Arabidopsis leaf development

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Gene expression

dexamethasone (Dex)-inducible system ... Arabidopsis transgenic plants that can express geminivirus RepA protein (RepAwt) in an inducible manner ... we determined by real-time reverse transcription (RT)-PCR the mRNA levels of ... after induction of the viral protein ... E2Fa ... was up-regulated in these plants

Desvoyes B, Ramirez-Parra E, Xie Q, Chua NH, Gutierrez C - Cell type-specific role of the retinoblastoma/E2F pathway during Arabidopsis leaf development

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Gene expression

dexamethasone (Dex)-inducible system ... Arabidopsis transgenic plants that can express geminivirus RepA protein (RepAwt) in an inducible manner ... expressing a translational fusion of the β-glucuronidase (GUS) reporter gene with the destruction box of cyclin B1;1, a useful G2/M marker ... after induction by Dex, we observed a stronger GUS expression ... in the proximal zone of young leaves

Desvoyes B, Ramirez-Parra E, Xie Q, Chua NH, Gutierrez C - Cell type-specific role of the retinoblastoma/E2F pathway during Arabidopsis leaf development

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Gene expression

dexamethasone (Dex)-inducible system ... Arabidopsis transgenic plants that can express geminivirus RepA protein (RepAwt) in an inducible manner ... members of the ORC (origin recognition complex ... expression ... increased in the RepAwt-expressing plants

Desvoyes B, Ramirez-Parra E, Xie Q, Chua NH, Gutierrez C - Cell type-specific role of the retinoblastoma/E2F pathway during Arabidopsis leaf development

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Gene expression

BAM1, BAM2 and BAM3 were broadly expressed throughout the different tissues and developmental stages examined

DeYoung BJ, Bickle KL, Schrage KJ, Muskett P, Patel K, Clark SE - The CLAVATA1-related BAM1, BAM2 and BAM3 receptor kinase-like proteins are required for meristem function in Arabidopsis

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Gene expression

A robust signal for both BAM1 and BAM2 genes could be detected on the flanks of the meristem (Figure 5)

DeYoung BJ, Bickle KL, Schrage KJ, Muskett P, Patel K, Clark SE - The CLAVATA1-related BAM1, BAM2 and BAM3 receptor kinase-like proteins are required for meristem function in Arabidopsis

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Gene expression

BAM1, BAM2 and BAM3 were broadly expressed throughout the different tissues and developmental stages examined

DeYoung BJ, Bickle KL, Schrage KJ, Muskett P, Patel K, Clark SE - The CLAVATA1-related BAM1, BAM2 and BAM3 receptor kinase-like proteins are required for meristem function in Arabidopsis

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Gene expression

BAM1, BAM2 and BAM3 were broadly expressed throughout the different tissues and developmental stages examined

DeYoung BJ, Bickle KL, Schrage KJ, Muskett P, Patel K, Clark SE - The CLAVATA1-related BAM1, BAM2 and BAM3 receptor kinase-like proteins are required for meristem function in Arabidopsis

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Gene expression

A robust signal for both BAM1 and BAM2 genes could be detected on the flanks of the meristem (Figure 5

DeYoung BJ, Bickle KL, Schrage KJ, Muskett P, Patel K, Clark SE - The CLAVATA1-related BAM1, BAM2 and BAM3 receptor kinase-like proteins are required for meristem function in Arabidopsis

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Gene expression

Genes expressed to higher levels in the cyp98A3 mutant were clearly over-represented in the functional group related to jasmonate signaling (Fig. 8B). Indeed, many genes involved in the biosynthesis of jasmonate are significantly higher expressed in the mutant compared to wild type (Ws); These include LOX2 (see above), LOX1, allene oxide synthase (CYP74A, At5g42650), and allene oxide cyclase 4 (At1g13280). In addition, genes related to the abscisic acid cascade are over-represented in the group of genes expressed to higher levels in cyp98A3 insertion mutants (Fig. 8B). These include the gene responsive to dehydration 22 (RD22I, At5g25610), which is strongly induced by abscisic acid and drought (Yamaguchi-Shinozaki and Shinozaki, 1993), and the Arg decarboxylase 2 (ADC2, At4g34710) gene, which is strongly activated by abscisic acid, but also by methyl jasmonate and mechanical wounding, and is involved in polyamine biosynthesis (Perez-Amador et al., 2002). Also expressed to higher levels in cyp98A3 insertion mutants were two homeodomain Leu-zipper transcription factors (ATHB5, At5g65310; and ATHB7, At2g46680) involved in abscisic acid-mediated signaling pathways related to drought and osmotic stress (Söderman et al., 1996) and seed germination (Johannesson et al., 2003), respectively

Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth

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Gene expression

Indeed, several genes encoding proteins involved in the light-harvesting complex (e.g. chlorophyll a/b-binding proteins At2g34430, At3g08940, and At3g47470), in photosynthetic electron transport (e.g. the putative ferredoxin At1g10960 and proton gradient regulation 5, At2g05620; Munekage et al., 2002), and in the Calvin cycle (e.g. Rubisco At5g38410 and the putative Rib-5-P isomerase At3g04790) are strongly down-regulated in the mutant compared to the wild type (Supplemental Table I

Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth

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Gene expression

In contrast, genes expressed to higher levels in cyp98A3 insertion mutants compared to wild type were over-represented in functional categories covering carbohydrate metabolism and include genes encoding enzymes in glycolysis (e.g. the glyceraldehyde 3-P dehydrogenase, At3g04120), the tricarboxylic acid cycle (e.g. the phosphoenolpyruvate carboxylase, At3g14940), and other anaerobic metabolisms (alcohol dehydrogenase, At1g77120). Also, functional categories related to defense responses were over-represented (Fig. 8A). Among these were genes known to be transcriptionally induced by insect feeding (the β-glucosidase BGL1, At1g52400; and the lipoxygenase LOX2, At3g45140; Stotz et al., 2000) or wounding/jasmonate (e.g. jasmonate response JR1, At3g16470; Leon et al., 1998). Finally, genes involved in secondary metabolism are over-represented in the group of up-regulated genes (Fig. 8A). It is noteworthy that genes encoding enzymes involved in flavonoid and anthocyanin biosynthesis and transport are expressed to higher levels in the cyp98A3 insertion mutant; These include chalcone isomerase (CHI, At3g55120), dihydroflavonol 4-reductase (DFR, At5g42800), flavanone 3-hydroxylase (F3H, At3g51240), and gluthation transferase (TT19, At5g17220; Weisshaar and Jenkins, 1998; Kitamura et al., 2004

Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth

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Gene expression

While transcript levels of genes encoding Phe ammonia lyase (PAL1; At2g37040) and HCT (At5g48930; Fig. 7A) were comparable in cyp98A3 mutants and wild type at all time points, an increase in C4H (CYP73A5; At2g30490) expression compared to wild type was observed at the 2-, 4-, and 8-h time points in cyp98A3 insertion mutants (Fig. 7A

Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth

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Gene expression

Genes expressed to higher levels in the cyp98A3 mutant were clearly over-represented in the functional group related to jasmonate signaling (Fig. 8B). Indeed, many genes involved in the biosynthesis of jasmonate are significantly higher expressed in the mutant compared to wild type (Ws); These include LOX2 (see above), LOX1, allene oxide synthase (CYP74A, At5g42650), and allene oxide cyclase 4 (At1g13280). In addition, genes related to the abscisic acid cascade are over-represented in the group of genes expressed to higher levels in cyp98A3 insertion mutants (Fig. 8B). These include the gene responsive to dehydration 22 (RD22I, At5g25610), which is strongly induced by abscisic acid and drought (Yamaguchi-Shinozaki and Shinozaki, 1993), and the Arg decarboxylase 2 (ADC2, At4g34710) gene, which is strongly activated by abscisic acid, but also by methyl jasmonate and mechanical wounding, and is involved in polyamine biosynthesis (Perez-Amador et al., 2002). Also expressed to higher levels in cyp98A3 insertion mutants were two homeodomain Leu-zipper transcription factors (ATHB5, At5g65310; and ATHB7, At2g46680) involved in abscisic acid-mediated signaling pathways related to drought and osmotic stress (Söderman et al., 1996) and seed germination (Johannesson et al., 2003), respectively

Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth

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Gene expression

Genes expressed to higher levels in the cyp98A3 mutant were clearly over-represented in the functional group related to jasmonate signaling (Fig. 8B). Indeed, many genes involved in the biosynthesis of jasmonate are significantly higher expressed in the mutant compared to wild type (Ws); These include LOX2 (see above), LOX1, allene oxide synthase (CYP74A, At5g42650), and allene oxide cyclase 4 (At1g13280). In addition, genes related to the abscisic acid cascade are over-represented in the group of genes expressed to higher levels in cyp98A3 insertion mutants (Fig. 8B). These include the gene responsive to dehydration 22 (RD22I, At5g25610), which is strongly induced by abscisic acid and drought (Yamaguchi-Shinozaki and Shinozaki, 1993), and the Arg decarboxylase 2 (ADC2, At4g34710) gene, which is strongly activated by abscisic acid, but also by methyl jasmonate and mechanical wounding, and is involved in polyamine biosynthesis (Perez-Amador et al., 2002). Also expressed to higher levels in cyp98A3 insertion mutants were two homeodomain Leu-zipper transcription factors (ATHB5, At5g65310; and ATHB7, At2g46680) involved in abscisic acid-mediated signaling pathways related to drought and osmotic stress (Söderman et al., 1996) and seed germination (Johannesson et al., 2003), respectively

Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth

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Gene expression

Indeed, several genes encoding proteins involved in the light-harvesting complex (e.g. chlorophyll a/b-binding proteins At2g34430, At3g08940, and At3g47470), in photosynthetic electron transport (e.g. the putative ferredoxin At1g10960 and proton gradient regulation 5, At2g05620; Munekage et al., 2002), and in the Calvin cycle (e.g. Rubisco At5g38410 and the putative Rib-5-P isomerase At3g04790) are strongly down-regulated in the mutant compared to the wild type (Supplemental Table I

Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth

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Gene expression

Indeed, several genes encoding proteins involved in the light-harvesting complex (e.g. chlorophyll a/b-binding proteins At2g34430, At3g08940, and At3g47470), in photosynthetic electron transport (e.g. the putative ferredoxin At1g10960 and proton gradient regulation 5, At2g05620; Munekage et al., 2002), and in the Calvin cycle (e.g. Rubisco At5g38410 and the putative Rib-5-P isomerase At3g04790) are strongly down-regulated in the mutant compared to the wild type (Supplemental Table I

Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth

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Gene expression

Six of the 28 expansin family members represented on the array used are differentially expressed, significantly more than expected by chance. However, some expansin isoforms (EXP1, At1g69530; EXP10, At1g26770; and EXPR, At4g17030) are expressed to higher levels in cyp98A3 insertion mutants, whereas others (EXP8, At2g40610; EXP3, At2g37640; and EXP11, At1g20190) are down-regulated compared to levels found in wild-type plants

Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth

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Gene expression

In contrast, genes expressed to higher levels in cyp98A3 insertion mutants compared to wild type were over-represented in functional categories covering carbohydrate metabolism and include genes encoding enzymes in glycolysis (e.g. the glyceraldehyde 3-P dehydrogenase, At3g04120), the tricarboxylic acid cycle (e.g. the phosphoenolpyruvate carboxylase, At3g14940), and other anaerobic metabolisms (alcohol dehydrogenase, At1g77120). Also, functional categories related to defense responses were over-represented (Fig. 8A). Among these were genes known to be transcriptionally induced by insect feeding (the β-glucosidase BGL1, At1g52400; and the lipoxygenase LOX2, At3g45140; Stotz et al., 2000) or wounding/jasmonate (e.g. jasmonate response JR1, At3g16470; Leon et al., 1998). Finally, genes involved in secondary metabolism are over-represented in the group of up-regulated genes (Fig. 8A). It is noteworthy that genes encoding enzymes involved in flavonoid and anthocyanin biosynthesis and transport are expressed to higher levels in the cyp98A3 insertion mutant; These include chalcone isomerase (CHI, At3g55120), dihydroflavonol 4-reductase (DFR, At5g42800), flavanone 3-hydroxylase (F3H, At3g51240), and gluthation transferase (TT19, At5g17220; Weisshaar and Jenkins, 1998; Kitamura et al., 2004

Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth

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Gene expression

Indeed, several genes encoding proteins involved in the light-harvesting complex (e.g. chlorophyll a/b-binding proteins At2g34430, At3g08940, and At3g47470), in photosynthetic electron transport (e.g. the putative ferredoxin At1g10960 and proton gradient regulation 5, At2g05620; Munekage et al., 2002), and in the Calvin cycle (e.g. Rubisco At5g38410 and the putative Rib-5-P isomerase At3g04790) are strongly down-regulated in the mutant compared to the wild type (Supplemental Table I

Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth

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Gene expression

In contrast, genes expressed to higher levels in cyp98A3 insertion mutants compared to wild type were over-represented in functional categories covering carbohydrate metabolism and include genes encoding enzymes in glycolysis (e.g. the glyceraldehyde 3-P dehydrogenase, At3g04120), the tricarboxylic acid cycle (e.g. the phosphoenolpyruvate carboxylase, At3g14940), and other anaerobic metabolisms (alcohol dehydrogenase, At1g77120). Also, functional categories related to defense responses were over-represented (Fig. 8A). Among these were genes known to be transcriptionally induced by insect feeding (the β-glucosidase BGL1, At1g52400; and the lipoxygenase LOX2, At3g45140; Stotz et al., 2000) or wounding/jasmonate (e.g. jasmonate response JR1, At3g16470; Leon et al., 1998). Finally, genes involved in secondary metabolism are over-represented in the group of up-regulated genes (Fig. 8A). It is noteworthy that genes encoding enzymes involved in flavonoid and anthocyanin biosynthesis and transport are expressed to higher levels in the cyp98A3 insertion mutant; These include chalcone isomerase (CHI, At3g55120), dihydroflavonol 4-reductase (DFR, At5g42800), flavanone 3-hydroxylase (F3H, At3g51240), and gluthation transferase (TT19, At5g17220; Weisshaar and Jenkins, 1998; Kitamura et al., 2004

Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth

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Gene expression

In contrast, genes expressed to higher levels in cyp98A3 insertion mutants compared to wild type were over-represented in functional categories covering carbohydrate metabolism and include genes encoding enzymes in glycolysis (e.g. the glyceraldehyde 3-P dehydrogenase, At3g04120), the tricarboxylic acid cycle (e.g. the phosphoenolpyruvate carboxylase, At3g14940), and other anaerobic metabolisms (alcohol dehydrogenase, At1g77120). Also, functional categories related to defense responses were over-represented (Fig. 8A). Among these were genes known to be transcriptionally induced by insect feeding (the β-glucosidase BGL1, At1g52400; and the lipoxygenase LOX2, At3g45140; Stotz et al., 2000) or wounding/jasmonate (e.g. jasmonate response JR1, At3g16470; Leon et al., 1998). Finally, genes involved in secondary metabolism are over-represented in the group of up-regulated genes (Fig. 8A). It is noteworthy that genes encoding enzymes involved in flavonoid and anthocyanin biosynthesis and transport are expressed to higher levels in the cyp98A3 insertion mutant; These include chalcone isomerase (CHI, At3g55120), dihydroflavonol 4-reductase (DFR, At5g42800), flavanone 3-hydroxylase (F3H, At3g51240), and gluthation transferase (TT19, At5g17220; Weisshaar and Jenkins, 1998; Kitamura et al., 2004

Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth

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Gene expression

Six of the 28 expansin family members represented on the array used are differentially expressed, significantly more than expected by chance. However, some expansin isoforms (EXP1, At1g69530; EXP10, At1g26770; and EXPR, At4g17030) are expressed to higher levels in cyp98A3 insertion mutants, whereas others (EXP8, At2g40610; EXP3, At2g37640; and EXP11, At1g20190) are down-regulated compared to levels found in wild-type plants

Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth

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Gene expression

Indeed, several genes encoding proteins involved in the light-harvesting complex (e.g. chlorophyll a/b-binding proteins At2g34430, At3g08940, and At3g47470), in photosynthetic electron transport (e.g. the putative ferredoxin At1g10960 and proton gradient regulation 5, At2g05620; Munekage et al., 2002), and in the Calvin cycle (e.g. Rubisco At5g38410 and the putative Rib-5-P isomerase At3g04790) are strongly down-regulated in the mutant compared to the wild type (Supplemental Table I

Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth

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Gene expression

Six of the 28 expansin family members represented on the array used are differentially expressed, significantly more than expected by chance. However, some expansin isoforms (EXP1, At1g69530; EXP10, At1g26770; and EXPR, At4g17030) are expressed to higher levels in cyp98A3 insertion mutants, whereas others (EXP8, At2g40610; EXP3, At2g37640; and EXP11, At1g20190) are down-regulated compared to levels found in wild-type plants

Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth

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Gene expression

In contrast, genes expressed to higher levels in cyp98A3 insertion mutants compared to wild type were over-represented in functional categories covering carbohydrate metabolism and include genes encoding enzymes in glycolysis (e.g. the glyceraldehyde 3-P dehydrogenase, At3g04120), the tricarboxylic acid cycle (e.g. the phosphoenolpyruvate carboxylase, At3g14940), and other anaerobic metabolisms (alcohol dehydrogenase, At1g77120). Also, functional categories related to defense responses were over-represented (Fig. 8A). Among these were genes known to be transcriptionally induced by insect feeding (the β-glucosidase BGL1, At1g52400; and the lipoxygenase LOX2, At3g45140; Stotz et al., 2000) or wounding/jasmonate (e.g. jasmonate response JR1, At3g16470; Leon et al., 1998). Finally, genes involved in secondary metabolism are over-represented in the group of up-regulated genes (Fig. 8A). It is noteworthy that genes encoding enzymes involved in flavonoid and anthocyanin biosynthesis and transport are expressed to higher levels in the cyp98A3 insertion mutant; These include chalcone isomerase (CHI, At3g55120), dihydroflavonol 4-reductase (DFR, At5g42800), flavanone 3-hydroxylase (F3H, At3g51240), and gluthation transferase (TT19, At5g17220; Weisshaar and Jenkins, 1998; Kitamura et al., 2004

Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth

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Gene expression

The total inactivation of the CYP98A3 gene in homozygous plants was confirmed by RNA-blot hybridization (Fig. 2C ) and quantitative reverse-transcription (RT)-PCR (see below

Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth

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Gene expression

Six of the 28 expansin family members represented on the array used are differentially expressed, significantly more than expected by chance. However, some expansin isoforms (EXP1, At1g69530; EXP10, At1g26770; and EXPR, At4g17030) are expressed to higher levels in cyp98A3 insertion mutants, whereas others (EXP8, At2g40610; EXP3, At2g37640; and EXP11, At1g20190) are down-regulated compared to levels found in wild-type plants

Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth

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Gene expression

Genes expressed to higher levels in the cyp98A3 mutant were clearly over-represented in the functional group related to jasmonate signaling (Fig. 8B). Indeed, many genes involved in the biosynthesis of jasmonate are significantly higher expressed in the mutant compared to wild type (Ws); These include LOX2 (see above), LOX1, allene oxide synthase (CYP74A, At5g42650), and allene oxide cyclase 4 (At1g13280). In addition, genes related to the abscisic acid cascade are over-represented in the group of genes expressed to higher levels in cyp98A3 insertion mutants (Fig. 8B). These include the gene responsive to dehydration 22 (RD22I, At5g25610), which is strongly induced by abscisic acid and drought (Yamaguchi-Shinozaki and Shinozaki, 1993), and the Arg decarboxylase 2 (ADC2, At4g34710) gene, which is strongly activated by abscisic acid, but also by methyl jasmonate and mechanical wounding, and is involved in polyamine biosynthesis (Perez-Amador et al., 2002). Also expressed to higher levels in cyp98A3 insertion mutants were two homeodomain Leu-zipper transcription factors (ATHB5, At5g65310; and ATHB7, At2g46680) involved in abscisic acid-mediated signaling pathways related to drought and osmotic stress (Söderman et al., 1996) and seed germination (Johannesson et al., 2003), respectively

Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth

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Gene expression

Six of the 28 expansin family members represented on the array used are differentially expressed, significantly more than expected by chance. However, some expansin isoforms (EXP1, At1g69530; EXP10, At1g26770; and EXPR, At4g17030) are expressed to higher levels in cyp98A3 insertion mutants, whereas others (EXP8, At2g40610; EXP3, At2g37640; and EXP11, At1g20190) are down-regulated compared to levels found in wild-type plants

Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth

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Gene expression

While transcript levels of genes encoding Phe ammonia lyase (PAL1; At2g37040) and HCT (At5g48930; Fig. 7A) were comparable in cyp98A3 mutants and wild type at all time points, an increase in C4H (CYP73A5; At2g30490) expression compared to wild type was observed at the 2-, 4-, and 8-h time points in cyp98A3 insertion mutants (Fig. 7A

Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth

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Gene expression

In contrast, genes expressed to higher levels in cyp98A3 insertion mutants compared to wild type were over-represented in functional categories covering carbohydrate metabolism and include genes encoding enzymes in glycolysis (e.g. the glyceraldehyde 3-P dehydrogenase, At3g04120), the tricarboxylic acid cycle (e.g. the phosphoenolpyruvate carboxylase, At3g14940), and other anaerobic metabolisms (alcohol dehydrogenase, At1g77120). Also, functional categories related to defense responses were over-represented (Fig. 8A). Among these were genes known to be transcriptionally induced by insect feeding (the β-glucosidase BGL1, At1g52400; and the lipoxygenase LOX2, At3g45140; Stotz et al., 2000) or wounding/jasmonate (e.g. jasmonate response JR1, At3g16470; Leon et al., 1998). Finally, genes involved in secondary metabolism are over-represented in the group of up-regulated genes (Fig. 8A). It is noteworthy that genes encoding enzymes involved in flavonoid and anthocyanin biosynthesis and transport are expressed to higher levels in the cyp98A3 insertion mutant; These include chalcone isomerase (CHI, At3g55120), dihydroflavonol 4-reductase (DFR, At5g42800), flavanone 3-hydroxylase (F3H, At3g51240), and gluthation transferase (TT19, At5g17220; Weisshaar and Jenkins, 1998; Kitamura et al., 2004

Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth

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Gene expression

Indeed, several genes encoding proteins involved in the light-harvesting complex (e.g. chlorophyll a/b-binding proteins At2g34430, At3g08940, and At3g47470), in photosynthetic electron transport (e.g. the putative ferredoxin At1g10960 and proton gradient regulation 5, At2g05620; Munekage et al., 2002), and in the Calvin cycle (e.g. Rubisco At5g38410 and the putative Rib-5-P isomerase At3g04790) are strongly down-regulated in the mutant compared to the wild type (Supplemental Table I

Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth

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Gene expression

While transcript levels of genes encoding Phe ammonia lyase (PAL1; At2g37040) and HCT (At5g48930; Fig. 7A) were comparable in cyp98A3 mutants and wild type at all time points, an increase in C4H (CYP73A5; At2g30490) expression compared to wild type was observed at the 2-, 4-, and 8-h time points in cyp98A3 insertion mutants (Fig. 7A

Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth

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Gene expression

In contrast, genes expressed to higher levels in cyp98A3 insertion mutants compared to wild type were over-represented in functional categories covering carbohydrate metabolism and include genes encoding enzymes in glycolysis (e.g. the glyceraldehyde 3-P dehydrogenase, At3g04120), the tricarboxylic acid cycle (e.g. the phosphoenolpyruvate carboxylase, At3g14940), and other anaerobic metabolisms (alcohol dehydrogenase, At1g77120). Also, functional categories related to defense responses were over-represented (Fig. 8A). Among these were genes known to be transcriptionally induced by insect feeding (the β-glucosidase BGL1, At1g52400; and the lipoxygenase LOX2, At3g45140; Stotz et al., 2000) or wounding/jasmonate (e.g. jasmonate response JR1, At3g16470; Leon et al., 1998). Finally, genes involved in secondary metabolism are over-represented in the group of up-regulated genes (Fig. 8A). It is noteworthy that genes encoding enzymes involved in flavonoid and anthocyanin biosynthesis and transport are expressed to higher levels in the cyp98A3 insertion mutant; These include chalcone isomerase (CHI, At3g55120), dihydroflavonol 4-reductase (DFR, At5g42800), flavanone 3-hydroxylase (F3H, At3g51240), and gluthation transferase (TT19, At5g17220; Weisshaar and Jenkins, 1998; Kitamura et al., 2004

Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth

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Gene expression

In contrast, genes expressed to higher levels in cyp98A3 insertion mutants compared to wild type were over-represented in functional categories covering carbohydrate metabolism and include genes encoding enzymes in glycolysis (e.g. the glyceraldehyde 3-P dehydrogenase, At3g04120), the tricarboxylic acid cycle (e.g. the phosphoenolpyruvate carboxylase, At3g14940), and other anaerobic metabolisms (alcohol dehydrogenase, At1g77120). Also, functional categories related to defense responses were over-represented (Fig. 8A). Among these were genes known to be transcriptionally induced by insect feeding (the β-glucosidase BGL1, At1g52400; and the lipoxygenase LOX2, At3g45140; Stotz et al., 2000) or wounding/jasmonate (e.g. jasmonate response JR1, At3g16470; Leon et al., 1998). Finally, genes involved in secondary metabolism are over-represented in the group of up-regulated genes (Fig. 8A). It is noteworthy that genes encoding enzymes involved in flavonoid and anthocyanin biosynthesis and transport are expressed to higher levels in the cyp98A3 insertion mutant; These include chalcone isomerase (CHI, At3g55120), dihydroflavonol 4-reductase (DFR, At5g42800), flavanone 3-hydroxylase (F3H, At3g51240), and gluthation transferase (TT19, At5g17220; Weisshaar and Jenkins, 1998; Kitamura et al., 2004

Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth

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Gene expression

Six of the 28 expansin family members represented on the array used are differentially expressed, significantly more than expected by chance. However, some expansin isoforms (EXP1, At1g69530; EXP10, At1g26770; and EXPR, At4g17030) are expressed to higher levels in cyp98A3 insertion mutants, whereas others (EXP8, At2g40610; EXP3, At2g37640; and EXP11, At1g20190) are down-regulated compared to levels found in wild-type plants

Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth

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Gene expression

Indeed, several genes encoding proteins involved in the light-harvesting complex (e.g. chlorophyll a/b-binding proteins At2g34430, At3g08940, and At3g47470), in photosynthetic electron transport (e.g. the putative ferredoxin At1g10960 and proton gradient regulation 5, At2g05620; Munekage et al., 2002), and in the Calvin cycle (e.g. Rubisco At5g38410 and the putative Rib-5-P isomerase At3g04790) are strongly down-regulated in the mutant compared to the wild type (Supplemental Table I

Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth

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Gene expression

In contrast, genes expressed to higher levels in cyp98A3 insertion mutants compared to wild type were over-represented in functional categories covering carbohydrate metabolism and include genes encoding enzymes in glycolysis (e.g. the glyceraldehyde 3-P dehydrogenase, At3g04120), the tricarboxylic acid cycle (e.g. the phosphoenolpyruvate carboxylase, At3g14940), and other anaerobic metabolisms (alcohol dehydrogenase, At1g77120). Also, functional categories related to defense responses were over-represented (Fig. 8A). Among these were genes known to be transcriptionally induced by insect feeding (the β-glucosidase BGL1, At1g52400; and the lipoxygenase LOX2, At3g45140; Stotz et al., 2000) or wounding/jasmonate (e.g. jasmonate response JR1, At3g16470; Leon et al., 1998). Finally, genes involved in secondary metabolism are over-represented in the group of up-regulated genes (Fig. 8A). It is noteworthy that genes encoding enzymes involved in flavonoid and anthocyanin biosynthesis and transport are expressed to higher levels in the cyp98A3 insertion mutant; These include chalcone isomerase (CHI, At3g55120), dihydroflavonol 4-reductase (DFR, At5g42800), flavanone 3-hydroxylase (F3H, At3g51240), and gluthation transferase (TT19, At5g17220; Weisshaar and Jenkins, 1998; Kitamura et al., 2004

Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth

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Gene expression

Genes expressed to higher levels in the cyp98A3 mutant were clearly over-represented in the functional group related to jasmonate signaling (Fig. 8B). Indeed, many genes involved in the biosynthesis of jasmonate are significantly higher expressed in the mutant compared to wild type (Ws); These include LOX2 (see above), LOX1, allene oxide synthase (CYP74A, At5g42650), and allene oxide cyclase 4 (At1g13280). In addition, genes related to the abscisic acid cascade are over-represented in the group of genes expressed to higher levels in cyp98A3 insertion mutants (Fig. 8B). These include the gene responsive to dehydration 22 (RD22I, At5g25610), which is strongly induced by abscisic acid and drought (Yamaguchi-Shinozaki and Shinozaki, 1993), and the Arg decarboxylase 2 (ADC2, At4g34710) gene, which is strongly activated by abscisic acid, but also by methyl jasmonate and mechanical wounding, and is involved in polyamine biosynthesis (Perez-Amador et al., 2002). Also expressed to higher levels in cyp98A3 insertion mutants were two homeodomain Leu-zipper transcription factors (ATHB5, At5g65310; and ATHB7, At2g46680) involved in abscisic acid-mediated signaling pathways related to drought and osmotic stress (Söderman et al., 1996) and seed germination (Johannesson et al., 2003), respectively

Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth

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Gene expression

Genes expressed to higher levels in the cyp98A3 mutant were clearly over-represented in the functional group related to jasmonate signaling (Fig. 8B). Indeed, many genes involved in the biosynthesis of jasmonate are significantly higher expressed in the mutant compared to wild type (Ws); These include LOX2 (see above), LOX1, allene oxide synthase (CYP74A, At5g42650), and allene oxide cyclase 4 (At1g13280). In addition, genes related to the abscisic acid cascade are over-represented in the group of genes expressed to higher levels in cyp98A3 insertion mutants (Fig. 8B). These include the gene responsive to dehydration 22 (RD22I, At5g25610), which is strongly induced by abscisic acid and drought (Yamaguchi-Shinozaki and Shinozaki, 1993), and the Arg decarboxylase 2 (ADC2, At4g34710) gene, which is strongly activated by abscisic acid, but also by methyl jasmonate and mechanical wounding, and is involved in polyamine biosynthesis (Perez-Amador et al., 2002). Also expressed to higher levels in cyp98A3 insertion mutants were two homeodomain Leu-zipper transcription factors (ATHB5, At5g65310; and ATHB7, At2g46680) involved in abscisic acid-mediated signaling pathways related to drought and osmotic stress (Söderman et al., 1996) and seed germination (Johannesson et al., 2003), respectively

Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth

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Gene expression

In contrast, genes expressed to higher levels in cyp98A3 insertion mutants compared to wild type were over-represented in functional categories covering carbohydrate metabolism and include genes encoding enzymes in glycolysis (e.g. the glyceraldehyde 3-P dehydrogenase, At3g04120), the tricarboxylic acid cycle (e.g. the phosphoenolpyruvate carboxylase, At3g14940), and other anaerobic metabolisms (alcohol dehydrogenase, At1g77120). Also, functional categories related to defense responses were over-represented (Fig. 8A). Among these were genes known to be transcriptionally induced by insect feeding (the β-glucosidase BGL1, At1g52400; and the lipoxygenase LOX2, At3g45140; Stotz et al., 2000) or wounding/jasmonate (e.g. jasmonate response JR1, At3g16470; Leon et al., 1998). Finally, genes involved in secondary metabolism are over-represented in the group of up-regulated genes (Fig. 8A). It is noteworthy that genes encoding enzymes involved in flavonoid and anthocyanin biosynthesis and transport are expressed to higher levels in the cyp98A3 insertion mutant; These include chalcone isomerase (CHI, At3g55120), dihydroflavonol 4-reductase (DFR, At5g42800), flavanone 3-hydroxylase (F3H, At3g51240), and gluthation transferase (TT19, At5g17220; Weisshaar and Jenkins, 1998; Kitamura et al., 2004

Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth

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Gene expression

MIF1 expression was undetectable in the rosette leaf blade

Hu W, Ma H - Characterization of a novel putative zinc finger gene MIF1: involvement in multiple hormonal regulation of Arabidopsis development

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Gene expression

in 35S::MIF1 seedlings ... At1g62500, a member of the lipid transfer protein family that is similar to an auxin down-regulated gene in soybean (Glycine max), was found to be up-regulated

Hu W, Ma H - Characterization of a novel putative zinc finger gene MIF1: involvement in multiple hormonal regulation of Arabidopsis development

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Gene expression

The expression of ... the gibberellin-responsive GASA gene family ... At1g22690 (GAST2 homolog ... was significantly down-regulated ... in 35S::MIF1 seedlings

Hu W, Ma H - Characterization of a novel putative zinc finger gene MIF1: involvement in multiple hormonal regulation of Arabidopsis development

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Gene expression

MIF1 was expressed ... very weakly in the petiole

Hu W, Ma H - Characterization of a novel putative zinc finger gene MIF1: involvement in multiple hormonal regulation of Arabidopsis development

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Gene expression

The expression of ... the gibberellin-responsive GASA gene family, At1g74670 (GAST1-like protein) ... was significantly down-regulated ... in 35S::MIF1 seedlings

Hu W, Ma H - Characterization of a novel putative zinc finger gene MIF1: involvement in multiple hormonal regulation of Arabidopsis development

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Gene expression

in 35S::MIF1 seedlings ... EXP2 ... up-regulated

Hu W, Ma H - Characterization of a novel putative zinc finger gene MIF1: involvement in multiple hormonal regulation of Arabidopsis development

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Gene expression

Reverse transcriptase–polymerase chain reaction (RT-PCR) analysis yielded results ... MIF1 expression was also detectable ... in the leaf, including the petiole

Hu W, Ma H - Characterization of a novel putative zinc finger gene MIF1: involvement in multiple hormonal regulation of Arabidopsis development

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Gene expression

increased transcript levels of ... RGL3 ... in 35S::MIF1 seedlings

Hu W, Ma H - Characterization of a novel putative zinc finger gene MIF1: involvement in multiple hormonal regulation of Arabidopsis development

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Gene expression

in 35S::MIF1 seedlings ... XTH11 ... up-regulated

Hu W, Ma H - Characterization of a novel putative zinc finger gene MIF1: involvement in multiple hormonal regulation of Arabidopsis development

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Gene expression

MIF2 was ... undetectable in the ... leaf

Hu W, Ma H - Characterization of a novel putative zinc finger gene MIF1: involvement in multiple hormonal regulation of Arabidopsis development

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Gene expression

ABI4 ... up-regulated in the dark-grown 35S::MIF1 seedlings

Hu W, Ma H - Characterization of a novel putative zinc finger gene MIF1: involvement in multiple hormonal regulation of Arabidopsis development

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Gene expression

ABI3 ... up-regulated in the dark-grown 35S::MIF1 seedlings

Hu W, Ma H - Characterization of a novel putative zinc finger gene MIF1: involvement in multiple hormonal regulation of Arabidopsis development

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Gene expression

increased transcript levels of RGL2 ... in 35S::MIF1 seedlings

Hu W, Ma H - Characterization of a novel putative zinc finger gene MIF1: involvement in multiple hormonal regulation of Arabidopsis development

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Gene expression

decreased expression of RGL1 ... in 35S::MIF1 seedlings

Hu W, Ma H - Characterization of a novel putative zinc finger gene MIF1: involvement in multiple hormonal regulation of Arabidopsis development

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Gene expression

The expression of ... the gibberellin-responsive GASA gene family ... At2g14900, was significantly down-regulated ... in 35S::MIF1 seedlings

Hu W, Ma H - Characterization of a novel putative zinc finger gene MIF1: involvement in multiple hormonal regulation of Arabidopsis development

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Gene expression

Two GA 20-oxidases were significantly up-regulated in the dark-grown 35S::MIF1 seedlings (Table 2), consistent with the notion that GA biosynthesis is usually subject to negative feedback regulation (Olszewski et al., 2002). No other genes involved in GA metabolism, including GA 3-oxidases and GA 2-oxidases, showed significantly altered expression

Hu W, Ma H - Characterization of a novel putative zinc finger gene MIF1: involvement in multiple hormonal regulation of Arabidopsis development

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Gene expression

bdg cells accumulate higher levels of CER1 (∼184%) ... transcripts compared with wild-type plants

Kurdyukov S, Faust A, Nawrath C, Bär S, Voisin D, Efremova N, Franke R, Schreiber L, Saedler H, Métraux JP, Yephremov A - The epidermis-specific extracellular BODYGUARD controls cuticle development and morphogenesis in Arabidopsis

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Gene expression

With this method, the Alexa Fluor 488–specific fluorescent signals were detected in the epidermis of transgenic bdg BDG–Strep-tag plants (Figures 10B to 10G) but not in the bdg mutant (Figure 10A) or wild-type plants (data not shown). Although present throughout the epidermis of incipient and developing leaf primordia, BDG accumulated to higher levels on the adaxial side of organs (Figures 10B and 10C). In contrast with mRNA in situ hybridization (Figures 9F and 9G), a stronger signal intensity was not detected in the lateral regions of leaf primordia

Kurdyukov S, Faust A, Nawrath C, Bär S, Voisin D, Efremova N, Franke R, Schreiber L, Saedler H, Métraux JP, Yephremov A - The epidermis-specific extracellular BODYGUARD controls cuticle development and morphogenesis in Arabidopsis

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Gene expression

A polarly localized fluorescence pattern was observed in epidermal cells, suggesting that BDG contains a motif required for polar protein sorting (Figures 10D to 10G). In the epidermis, the epitope-specific signal was localized in the outermost cell wall layer

Kurdyukov S, Faust A, Nawrath C, Bär S, Voisin D, Efremova N, Franke R, Schreiber L, Saedler H, Métraux JP, Yephremov A - The epidermis-specific extracellular BODYGUARD controls cuticle development and morphogenesis in Arabidopsis

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Gene expression

Tissue sections prepared from vegetative meristems and reproductive organs of wild-type Arabidopsis plants were hybridized with riboprobes directed against BDG mRNA ... in all organs analyzed (leaf primordia, immature floral buds, sepals, petals, and anthers), expression was found to be limited to epidermal cells

Kurdyukov S, Faust A, Nawrath C, Bär S, Voisin D, Efremova N, Franke R, Schreiber L, Saedler H, Métraux JP, Yephremov A - The epidermis-specific extracellular BODYGUARD controls cuticle development and morphogenesis in Arabidopsis

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Gene expression

RNA gel blot hybridization revealed that whereas BDG is expressed in various organs, including roots, the strongest expression is detected in flowers and floral buds (see Supplemental Figure 2 online). Cauline leaves show higher levels of BDG transcripts than do rosette leaves

Kurdyukov S, Faust A, Nawrath C, Bär S, Voisin D, Efremova N, Franke R, Schreiber L, Saedler H, Métraux JP, Yephremov A - The epidermis-specific extracellular BODYGUARD controls cuticle development and morphogenesis in Arabidopsis

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Gene expression

In all organs of the BDG-GFP plants, GFP fluorescence was detected exclusively in protodermal and epidermal cells

Kurdyukov S, Faust A, Nawrath C, Bär S, Voisin D, Efremova N, Franke R, Schreiber L, Saedler H, Métraux JP, Yephremov A - The epidermis-specific extracellular BODYGUARD controls cuticle development and morphogenesis in Arabidopsis

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Gene expression

bdg cells accumulate higher levels of ... SHN1/WIN1 (∼158%) transcripts compared with wild-type plants

Kurdyukov S, Faust A, Nawrath C, Bär S, Voisin D, Efremova N, Franke R, Schreiber L, Saedler H, Métraux JP, Yephremov A - The epidermis-specific extracellular BODYGUARD controls cuticle development and morphogenesis in Arabidopsis

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Gene expression

Leaf primordia exhibited higher levels of BDG expression in lateral regions

Kurdyukov S, Faust A, Nawrath C, Bär S, Voisin D, Efremova N, Franke R, Schreiber L, Saedler H, Métraux JP, Yephremov A - The epidermis-specific extracellular BODYGUARD controls cuticle development and morphogenesis in Arabidopsis

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Gene expression

RNA in situ hybridization on seedlings and inflorescences reveals BB expression mainly in proliferating tissues, i.e., shoot and floral meristems and young organs

Disch S, Anastasiou E, Sharma VK, Laux T, Fletcher JC, Lenhard M - The E3 ubiquitin ligase BIG BROTHER controls arabidopsis organ size in a dosage-dependent manner

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Gene expression

By RT-PCR, BB mRNA can be detected in all plant organs tested

Disch S, Anastasiou E, Sharma VK, Laux T, Fletcher JC, Lenhard M - The E3 ubiquitin ligase BIG BROTHER controls arabidopsis organ size in a dosage-dependent manner

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Gene expression

The BB promoter is also active ... in developing embryos

Disch S, Anastasiou E, Sharma VK, Laux T, Fletcher JC, Lenhard M - The E3 ubiquitin ligase BIG BROTHER controls arabidopsis organ size in a dosage-dependent manner

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Gene expression

BB expression ... in the vasculature

Disch S, Anastasiou E, Sharma VK, Laux T, Fletcher JC, Lenhard M - The E3 ubiquitin ligase BIG BROTHER controls arabidopsis organ size in a dosage-dependent manner

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Gene expression

To investigate whether ANT is nuclear localized, ANT was transiently expressed as a GFP fusion protein in leek epidermal cells. While GFP is present in both the cytoplasm and nucleus (Fig. 6a), the GFP–ANT protein is present exclusively in the nucleus (Fig. 6b

Krizek BA, Sulli C - Mapping sequences required for nuclear localization and the transcriptional activation function of the Arabidopsis protein AINTEGUMENTA

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Gene expression

Strong expression of ... TRN2 was detected in ... leaf primordia

Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana

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Gene expression

in trn leaves ... little PDR5:GUS activity was detected at or around the vascular system of 8-d-old leaves 1 and 2

Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana

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Gene expression

in trn leaves ... little PDR5:GUS activity was detected at or around the vascular system of 8-d-old leaves 1 and 2

Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana

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Gene expression

PCYCB1-1:GUS activity ... trn1-1 ... ectopic activity was noted in the vascular system

Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana

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Gene expression

TRN1 ... Expression was also detected in the lamina of the cotyledons

Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana

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Gene expression

Strong expression of TRN1 ... was detected in the SAM

Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana

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Gene expression

Strong expression of TRN1 ... was detected in the ... leaf primordia

Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana

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Gene expression

in trn leaves ... ectopic GUS activity ... in the ... mesophyll layers

Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana

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Gene expression

TRN1 ... expressed in ... leaves

Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana

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Gene expression

trn leaves were treated with α-naphthaleneacetic acid ... expression of PDR5:GUS ... more intense ... was observed in trn

Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana

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Gene expression

Strong expression of ... TRN2 was detected in the SAM

Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana

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Gene expression

trn leaves were treated with α-naphthaleneacetic acid ... expression of PDR5:GUS ... more intense ... was observed in trn

Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana

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Gene expression

TRN1 ... expressed in ... cell suspensions

Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana

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Gene expression

TRN2 ... Expression was also detected in the lamina of the cotyledons

Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana

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Gene expression

TRN1 ... expressed in seedlings

Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana

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Gene expression

topology prediction programs ... predict TRN1 to be cytosolic

Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana

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Gene expression

in trn leaves ... PDR5:GUS ... ectopic expression was observed along the margins

Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana

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Gene expression

in trn leaves ... PDR5:GUS ... ectopic expression was observed along the margins

Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana

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Gene expression

in trn leaves ... ectopic GUS activity ... in the ... mesophyll layers

Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana

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Gene expression

RbcS3 ... abundant in both young-rosettes, 2.5 weeks of age, and mature rosettes, 4 weeks of age, but decline in abundance between 4 and 5 weeks of age

Duguay J, Jamal S, Liu Z, Wang TW, Thompson JE - Leaf-specific suppression of deoxyhypusine synthase in Arabidopsis thaliana enhances growth without negative pleiotropic effects

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Gene expression

RbcS1 ... abundant in both young-rosettes, 2.5 weeks of age, and mature rosettes, 4 weeks of age, but decline in abundance between 4 and 5 weeks of age

Duguay J, Jamal S, Liu Z, Wang TW, Thompson JE - Leaf-specific suppression of deoxyhypusine synthase in Arabidopsis thaliana enhances growth without negative pleiotropic effects

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Gene expression

The temporal and spatial expression of DHS in Arabidopsis was examined by measuring GUS activity in transgenic plants expressing the transgene ProAtDHS:GUS. Quantitative fluorescence measurements of GUS in the developing 2nd rosette leaf pair revealed a small peak of DHS promoter activity at 3.5 weeks of age coincident with the onset of bolting and the initiation of nutrient translocation from rosette leaves, and a much more pronounced peak of activity at weeks 5 and 6, the period during which there is extensive cell death (Fig. 1). This bimodal pattern of DHS promoter activity temporally matches a similar pattern of DHS protein expression in senescing Arabidopsis rosettes observed previously

Duguay J, Jamal S, Liu Z, Wang TW, Thompson JE - Leaf-specific suppression of deoxyhypusine synthase in Arabidopsis thaliana enhances growth without negative pleiotropic effects

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Gene expression

RbcS2 ... abundant in both young-rosettes, 2.5 weeks of age, and mature rosettes, 4 weeks of age, but decline in abundance between 4 and 5 weeks of age

Duguay J, Jamal S, Liu Z, Wang TW, Thompson JE - Leaf-specific suppression of deoxyhypusine synthase in Arabidopsis thaliana enhances growth without negative pleiotropic effects

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Gene expression

Histochemical analysis of transgenic plants expressing ProAteIF5A1GUS indicated that AteIF5A1 is also expressed in the 2nd rosette leaf pair of 5-week-old plants

Duguay J, Jamal S, Liu Z, Wang TW, Thompson JE - Leaf-specific suppression of deoxyhypusine synthase in Arabidopsis thaliana enhances growth without negative pleiotropic effects

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Gene expression

We detected ICU4 transcripts in roots, vegetative leaves, shoots, flower buds, and open flowers of Col-0 by semiquantitative reverse transcription (RT)-PCR

Ochando I, Jover-Gil S, Ripoll JJ, Candela H, Vera A, Ponce MR, Martínez-Laborda A, Micol JL - Mutations in the microRNA complementarity site of the INCURVATA4 gene perturb meristem function and adaxialize lateral organs in arabidopsis

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Gene expression

The ICU4 gene has previously been shown to be expressed in the vasculature, as seen in plants carrying a pATHB-15::β-glucuronidase (GUS) transgene (Ohashi-Ito and Fukuda, 2003)

Ochando I, Jover-Gil S, Ripoll JJ, Candela H, Vera A, Ponce MR, Martínez-Laborda A, Micol JL - Mutations in the microRNA complementarity site of the INCURVATA4 gene perturb meristem function and adaxialize lateral organs in arabidopsis

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Gene expression

To test whether the gain-of-function icu4-1 mutation altered the leaf venation pattern, we studied the expression of the pATHB-8-GUS reporter transgene, which is restricted to provascular cells and has previously been used to characterize the development of the vascular system in leaves and stems (Baima et al., 1995; Kang and Dengler, 2002; Kang et al., 2003). The pattern of GUS staining revealed no differences between the wild-type and icu4-1/icu4-1 mutant plants

Ochando I, Jover-Gil S, Ripoll JJ, Candela H, Vera A, Ponce MR, Martínez-Laborda A, Micol JL - Mutations in the microRNA complementarity site of the INCURVATA4 gene perturb meristem function and adaxialize lateral organs in arabidopsis

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Gene expression

KNAT2 was found to be misexpressed, with a 3-fold up-regulation in ... icu4-1/icu4-1 leaves

Ochando I, Jover-Gil S, Ripoll JJ, Candela H, Vera A, Ponce MR, Martínez-Laborda A, Micol JL - Mutations in the microRNA complementarity site of the INCURVATA4 gene perturb meristem function and adaxialize lateral organs in arabidopsis

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Gene expression

ICU4 ... transcript levels were 8-fold higher in icu4-1/icu4-1 leaves than in En-2 leaves, as is to be expected if icu4-1 escapes cleavage by the miRNA machinery

Ochando I, Jover-Gil S, Ripoll JJ, Candela H, Vera A, Ponce MR, Martínez-Laborda A, Micol JL - Mutations in the microRNA complementarity site of the INCURVATA4 gene perturb meristem function and adaxialize lateral organs in arabidopsis

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Gene expression

Transcript levels of ICU4 were lower in the leaves than in the aerial tissues of En-2

Ochando I, Jover-Gil S, Ripoll JJ, Candela H, Vera A, Ponce MR, Martínez-Laborda A, Micol JL - Mutations in the microRNA complementarity site of the INCURVATA4 gene perturb meristem function and adaxialize lateral organs in arabidopsis

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Gene expression

FIL was not upregulated in as1

Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway

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Gene expression

ETT ... upregulated in ... rdr6

Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway

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Gene expression

ARF4 ... upregulated in ... rdr6 ... and further increased in ... double mutants with as1

Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway

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Gene expression

PHAVOLUTA ... Expression is re- stricted to the adaxial domain

Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway

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Gene expression

ARF4 ... upregulated in ... sgs3

Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway

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Gene expression

ARF4 ... upregulated significantly in ago7 as pre- viously described

Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway

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Gene expression

ETT ... upregulated in ago7

Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway

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Gene expression

ETT ... upregulated significantly in ago7 ... and were slightly further increased in as1 ago7 double mutants

Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway

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Gene expression

STM was also slightly upregulated in as1 whole seedlings, in contrast with leaves [35], but no additional upregulation was ob- served in the as1 ago7 double mutants, and the level of upregulation was too low to be detected by microarray analysis (see below

Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway

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Gene expression

in ... rdr6 ... double mutants with as1 ... FIL was upregulated

Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway

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Gene expression

ETT ... upregulated in ago7 ... and further increased in ... double mutants with as1

Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway

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Gene expression

ETT ... upregulated in ... sgs3 ... and further increased in ... double mutants with as1

Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway

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Gene expression

FIL was not upregulated ... in ago7

Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway

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Gene expression

We first assayed BP accumulation in as1 and as1 ago7 (Figure S2J), but we could not detect further overexpres- sion in the double mutants, although we could not rule out changes in transcript localization

Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway

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Gene expression

in ago7 ... double mutants with as1 ... FIL was upregulated

Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway

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Gene expression

REVOLUTA ... Expression is re- stricted to the adaxial domain

Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway

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Gene expression

ARF4 ... upregulated significantly in ago7 ... and were slightly further increased in as1 ago7 double mutants

Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway

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Gene expression

overexpression of FIL in the as1 ago7 double mutant was reduced in the as1 ago7 ett triple mutant

Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway

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Gene expression

in ... sgs3 ... double mutants with as1 ... FIL was upregulated

Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway

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Gene expression

in as1 ago7 ... FIL ... was strongly upregu- lated in the double mutant

Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway

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Gene expression

FIL ... was strongly upregu- lated in ... as2 rdr6

Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway

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Gene expression

We found that a gene-trap reporter gene integrated into the promoter of TAS3 was expressed on the adaxial side of early leaf primordia

Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway

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Gene expression

The microRNA miR165 ... is expressed in the abaxial domain in older primordia

Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway

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Gene expression

ETT ... upregulated in ... sgs3

Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway

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Gene expression

ETT ... upregulated in ... rdr6 ... and further increased in ... double mutants with as1

Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway

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Gene expression

BP was strongly upregulated in as1 mutants compared to wild-type plants

Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway

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Gene expression

ARF4 ... upregulated in ago7

Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway

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Gene expression

BP was strongly upregulated in as1 mutants compared to wild-type plants, but no additional upregu- lation was observed in as1 ago7 double mutants

Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway

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Gene expression

ARF4 ... upregulated in ago7 ... and further increased in ... double mutants with as1

Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway

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Gene expression

We found that a gene-trap reporter gene integrated into the promoter of TAS3 was expressed ... in stipules

Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway

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Gene expression

ETT ... upregulated significantly in ago7 as pre- viously described

Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway

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Gene expression

PHABULOSA ... Expression is re- stricted to the adaxial domain

Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway

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Gene expression

ARF4 ... upregulated in ... rdr6

Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway

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Gene expression

Using quantitative RT (qRT)-PCR, we studied the expression of the RpoTm ... in 4-d-old seedlings (showing only expanded cotyledons), 12-d-old seedlings (showing expanded cotyledons and four expanding leaves), 3-week-old rosettes (see Fig. 1, G and H), and roots ... found to be expressed in all the organs and stages analyzed in wild-type ... plants. In Col-0, RpoTm reached the highest level of expression in 12-d-old seedlings, where it was 1.9-, 1.5-, and 2.1-fold higher than in 4-d-old seedlings, rosettes, and roots, respectively

Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis

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Gene expression

qRT-PCR amplifications were performed on RNA extracted from 4- and 12-d-old seedlings and 21-d-old rosettes of Col-0 and the sca3-2 mutant ... sca3-2 mutant ... rpoC1 ... down-regulated in ... all the developmental stages studied

Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis

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Gene expression

In the sca3-2 mutant, the LCHB2 ... 6.4 ... fold down-regulated by qRT-PCR, whereas microarray analyses showed 3.7 ... fold levels

Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis

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Gene expression

Arabidopsis in 4-d-old seedlings (showing only expanded cotyledons), 12-d-old seedlings (showing expanded cotyledons and four expanding leaves), 3-week-old rosettes (see Fig. 1, G and H), and roots ... in ... sca3-2/sca3-2 plants ... RpoTm ... reduction was found ... in 12-d-old seedlings

Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis

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Gene expression

In the sca3-2 mutant ... PSAK ... 2.8 ... fold down-regulated by qRT-PCR, whereas microarray analyses showed ... 2.1 ... fold levels

Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis

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Gene expression

qRT-PCR amplifications were performed on RNA extracted from 4- and 12-d-old seedlings and 21-d-old rosettes of Col-0 and the sca3-2 mutant ... transcript levels were reduced in sca3-2/sca3-2 ... of rps18 only in 4-d-old seedlings

Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis

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Gene expression

qRT-PCR amplifications were performed on RNA extracted from 4- and 12-d-old seedlings and 21-d-old rosettes of Col-0 and the sca3-2 mutant ... transcript levels of ... clpP were reduced in 4-d-old seedlings ... in sca3-2/sca3-2

Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis

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Gene expression

Arabidopsis in 4-d-old seedlings (showing only expanded cotyledons), 12-d-old seedlings (showing expanded cotyledons and four expanding leaves), 3-week-old rosettes (see Fig. 1, G and H), and roots ... in ... sca3-2/sca3-2 plants ... RpoTmp ... a reduction was found ... in 12-d-old seedlings

Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis

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Gene expression

qRT-PCR amplifications were performed on RNA extracted from 4- and 12-d-old seedlings and 21-d-old rosettes of Col-0 and the sca3-2 mutant ... transcript levels of accD and ... were ... increased in 12-d-old seedlings ... in sca3-2/sca3-2

Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis

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Gene expression

down-regulated in sca3-2 ... CLB6

Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis

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Gene expression

down-regulated in sca3-2 ... CHLOROPLAST BIOGENESIS 4 ... CLB4; hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis

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Gene expression

Using quantitative RT (qRT)-PCR, we studied the expression ... SCA3 (RpoTp) ... 4-d-old seedlings (showing only expanded cotyledons), 12-d-old seedlings (showing expanded cotyledons and four expanding leaves), 3-week-old rosettes (see Fig. 1, G and H), and roots ... found to be expressed in all the organs and stages analyzed in wild-type ... SCA3 ... predominantly expressed in rosettes ... transcripts accumulated 3.3-, 2.5-, and 2.8-fold higher than in 4- and 12-d-old seedlings and roots, respectively

Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis

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Gene expression

To validate our microarray results, we used qRT-PCR to analyze the expression of five genes in 3-week-old sca3-2/sca3-2 and Col-0 plants. SEP3 was 7.0- and 2.7-fold up-regulated in sca3-2 compared with Col-0, as detected by qRT-PCR and microarray analysis, respectively

Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis

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Gene expression

qRT-PCR amplifications were performed on RNA extracted from 4- and 12-d-old seedlings and 21-d-old rosettes of Col-0 and the sca3-2 mutant ... transcript levels of ... clpP were ... increased in 12-d-old seedlings ... in sca3-2/sca3-2

Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis

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Gene expression

down-regulated in sca3-2 ... the RNAP σ-subunit SigA ... SIG1

Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis

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Gene expression

Arabidopsis in 4-d-old seedlings (showing only expanded cotyledons), 12-d-old seedlings (showing expanded cotyledons and four expanding leaves), 3-week-old rosettes (see Fig. 1, G and H), and roots ... in ... sca3-2/sca3-2 plants ... RpoTm ... showed higher transcript levels than in Col-0 in 4-d-old seedlings and 21-d-old plants

Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis

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Gene expression

qRT-PCR amplifications were performed on RNA extracted from 4- and 12-d-old seedlings and 21-d-old rosettes of Col-0 and the sca3-2 mutant ... transcript levels of accD ... were reduced in 4-d-old seedlings ... in sca3-2/sca3-2

Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis

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Gene expression

qRT-PCR amplifications were performed on RNA extracted from 4- and 12-d-old seedlings and 21-d-old rosettes of Col-0 and the sca3-2 mutant ... sca3-2 mutant ... rpoB ... down-regulated in all the developmental stages studied

Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis

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Gene expression

down-regulated in sca3-2 ... CLA1 ... encoding the 1-deoxy-D-xylulose-5-P synthase

Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis

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Gene expression

clpP transcript levels were reduced in sca3-2/sca3-2 rosettes

Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis

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Gene expression

SCA3 was down-regulated in the mutant in all developmental stages studied, especially in 4-d-old seedlings

Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis

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Gene expression

down-regulated in sca3-2 ... NCED4

Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis

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Gene expression

down-regulated in sca3-2 ... ABSCISIC ACID1 ... ABA1; zeaxanthin epoxidase

Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis

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Gene expression

In the sca3-2 mutant, the ... SIG1 ... 2.1 ... fold down-regulated by qRT-PCR, whereas microarray analyses showed ... 1.7 ... fold levels

Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis

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Gene expression

Using quantitative RT (qRT)-PCR, we studied the expression of ... RpoTmp ... in 4-d-old seedlings (showing only expanded cotyledons), 12-d-old seedlings (showing expanded cotyledons and four expanding leaves), 3-week-old rosettes (see Fig. 1, G and H), and roots ... found to be expressed in all the organs and stages analyzed in wild-type ... RpoTmp ... predominantly expressed in rosettes

Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis

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Gene expression

we performed a microarray analysis using RNA extracted from 3-week-old plants of the sca3-2 ... the most up-regulated gene found in the microarray was the MADS-box family member SEP3

Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis

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Gene expression

Arabidopsis in 4-d-old seedlings (showing only expanded cotyledons), 12-d-old seedlings (showing expanded cotyledons and four expanding leaves), 3-week-old rosettes (see Fig. 1, G and H), and roots ... in ... sca3-2/sca3-2 plants ... RpoTmp showed higher transcript levels than in Col-0 in 4-d-old seedlings and 21-d-old plants

Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis

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Gene expression

SCA3 gene ... is targeted exclusively to plastids (Hedtke et al., 1997, 1999)

Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis

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Gene expression

In the sca3-2 mutant ... ABA1 ... 1.6-fold down-regulated by qRT-PCR, whereas microarray analyses showed ... 1.7-fold levels

Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis

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Gene expression

leaves of the sgs3-11 as2-101 ... contained ... reduced levels of REV transcripts

Xu L, Yang L, Pi L, Liu Q, Ling Q, Wang H, Poethig RS, Huang H - Genetic interaction between the AS1-AS2 and RDR6-SGS3-AGO7 pathways for leaf morphogenesis

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Gene expression

miR165/166 ... transcript levels were ... elevated in the rdr6-3 as2-101 leaves

Xu L, Yang L, Pi L, Liu Q, Ling Q, Wang H, Poethig RS, Huang H - Genetic interaction between the AS1-AS2 and RDR6-SGS3-AGO7 pathways for leaf morphogenesis

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Gene expression

REV transcripts were detected in the earlier stage leaf primordia in ... zip as1-101

Xu L, Yang L, Pi L, Liu Q, Ling Q, Wang H, Poethig RS, Huang H - Genetic interaction between the AS1-AS2 and RDR6-SGS3-AGO7 pathways for leaf morphogenesis

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Gene expression

leaves of the ... zip as2-101 ... contained ... reduced levels of REV transcripts

Xu L, Yang L, Pi L, Liu Q, Ling Q, Wang H, Poethig RS, Huang H - Genetic interaction between the AS1-AS2 and RDR6-SGS3-AGO7 pathways for leaf morphogenesis

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Gene expression

REV ... repressed ... in the rdr6-3 as2-101 leaves

Xu L, Yang L, Pi L, Liu Q, Ling Q, Wang H, Poethig RS, Huang H - Genetic interaction between the AS1-AS2 and RDR6-SGS3-AGO7 pathways for leaf morphogenesis

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Gene expression

PHB ... repressed ... in the rdr6-3 as2-101 leaves

Xu L, Yang L, Pi L, Liu Q, Ling Q, Wang H, Poethig RS, Huang H - Genetic interaction between the AS1-AS2 and RDR6-SGS3-AGO7 pathways for leaf morphogenesis

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Gene expression

leaves of the ... zip as2-101 ... double mutant plants contained dramatically enhanced miR165/ 166 levels

Xu L, Yang L, Pi L, Liu Q, Ling Q, Wang H, Poethig RS, Huang H - Genetic interaction between the AS1-AS2 and RDR6-SGS3-AGO7 pathways for leaf morphogenesis

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Gene expression

REV transcripts were detected in the earlier stage leaf primordia in ... sgs3-11 as2-101

Xu L, Yang L, Pi L, Liu Q, Ling Q, Wang H, Poethig RS, Huang H - Genetic interaction between the AS1-AS2 and RDR6-SGS3-AGO7 pathways for leaf morphogenesis

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Gene expression

FIL transcript levels were ... elevated in the rdr6-3 as2-101 leaves

Xu L, Yang L, Pi L, Liu Q, Ling Q, Wang H, Poethig RS, Huang H - Genetic interaction between the AS1-AS2 and RDR6-SGS3-AGO7 pathways for leaf morphogenesis

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Gene expression

leaves of the sgs3-11 as2-101 ... double mutant plants contained dramatically enhanced miR165/ 166 levels

Xu L, Yang L, Pi L, Liu Q, Ling Q, Wang H, Poethig RS, Huang H - Genetic interaction between the AS1-AS2 and RDR6-SGS3-AGO7 pathways for leaf morphogenesis

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Gene expression

rdr6-3 as2-101 ... leaves ... contained ... reduced levels of REV transcripts

Xu L, Yang L, Pi L, Liu Q, Ling Q, Wang H, Poethig RS, Huang H - Genetic interaction between the AS1-AS2 and RDR6-SGS3-AGO7 pathways for leaf morphogenesis

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Gene expression

REV transcripts were detected in the earlier stage leaf primordia in ... zip as2-101

Xu L, Yang L, Pi L, Liu Q, Ling Q, Wang H, Poethig RS, Huang H - Genetic interaction between the AS1-AS2 and RDR6-SGS3-AGO7 pathways for leaf morphogenesis

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Gene expression

REV transcripts were detected in the earlier stage leaf primordia in sgs3-11 as1-101

Xu L, Yang L, Pi L, Liu Q, Ling Q, Wang H, Poethig RS, Huang H - Genetic interaction between the AS1-AS2 and RDR6-SGS3-AGO7 pathways for leaf morphogenesis

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Gene expression

the FILAMENTOUS FLOWER (FIL) gene that is known to promote abaxial leaf identity (Bowman 2000, Eshed et al. 2001) was expressed more abundantly in the ago1-27 as2-101 leaves than in the ago1-27 and as2-101 single mutant leaves

Xu L, Yang L, Pi L, Liu Q, Ling Q, Wang H, Poethig RS, Huang H - Genetic interaction between the AS1-AS2 and RDR6-SGS3-AGO7 pathways for leaf morphogenesis

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Gene expression

To investigate OBP2 expression at the cell and tissue level, approximately 1 kb of the 5′ upstream regulatory region of OBP2 was fused to the E. coliβ-glucuronidase (GUS) reporter gene and transferred to the nuclear genome of Arabidopsis ... In leaves, GUS expression in the vasculature was strongest in phloem cells

Skirycz A, Reichelt M, Burow M, Birkemeyer C, Rolcik J, Kopka J, Zanor MI, Gershenzon J, Strnad M, Szopa J, Mueller-Roeber B, Witt I - DOF transcription factor AtDof1

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Gene expression

To reduce OBP2 transcript level, we transformed Arabidopsis plants with an RNAi–OBP2 construct ... CYP79 ... B3 ... significantly reduced in just one line

Skirycz A, Reichelt M, Burow M, Birkemeyer C, Rolcik J, Kopka J, Zanor MI, Gershenzon J, Strnad M, Szopa J, Mueller-Roeber B, Witt I - DOF transcription factor AtDof1

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Gene expression

mechanical wounding, which stimulates jasmonate biosynthesis, enhanced OBP2 transcript level

Skirycz A, Reichelt M, Burow M, Birkemeyer C, Rolcik J, Kopka J, Zanor MI, Gershenzon J, Strnad M, Szopa J, Mueller-Roeber B, Witt I - DOF transcription factor AtDof1

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Gene expression

To reduce OBP2 transcript level, we transformed Arabidopsis plants with an RNAi–OBP2 construct ... CYP79B2 ... significantly reduced in just one line

Skirycz A, Reichelt M, Burow M, Birkemeyer C, Rolcik J, Kopka J, Zanor MI, Gershenzon J, Strnad M, Szopa J, Mueller-Roeber B, Witt I - DOF transcription factor AtDof1

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Gene expression

Targeted expression analysis on 35S:OBP2 plants using quantitative real-time PCR (Q-RTPCR) confirmed the increased transcript levels of ... ATR1 ... identified by transcript profiling

Skirycz A, Reichelt M, Burow M, Birkemeyer C, Rolcik J, Kopka J, Zanor MI, Gershenzon J, Strnad M, Szopa J, Mueller-Roeber B, Witt I - DOF transcription factor AtDof1

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Gene expression

Targeted expression analysis on 35S:OBP2 plants using quantitative real-time PCR (Q-RTPCR ... showed increases for CYP83B1 ... which were not represented on the array

Skirycz A, Reichelt M, Burow M, Birkemeyer C, Rolcik J, Kopka J, Zanor MI, Gershenzon J, Strnad M, Szopa J, Mueller-Roeber B, Witt I - DOF transcription factor AtDof1

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Gene expression

Using semi-quantitative RT-PCR, OBP2 was previously found to be expressed in all organs of 6-week-old Arabidopsis plants, revealing the highest expression in roots and leaves, and a comparatively weak expression in stems and flowers

Skirycz A, Reichelt M, Burow M, Birkemeyer C, Rolcik J, Kopka J, Zanor MI, Gershenzon J, Strnad M, Szopa J, Mueller-Roeber B, Witt I - DOF transcription factor AtDof1

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Gene expression

To reduce OBP2 transcript level, we transformed Arabidopsis plants with an RNAi–OBP2 construct ... CYP83B1 was significantly reduced in two lines

Skirycz A, Reichelt M, Burow M, Birkemeyer C, Rolcik J, Kopka J, Zanor MI, Gershenzon J, Strnad M, Szopa J, Mueller-Roeber B, Witt I - DOF transcription factor AtDof1

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Gene expression

Targeted expression analysis on 35S:OBP2 plants using quantitative real-time PCR (Q-RTPCR ... showed increases for ... MAM-1 ... which were not represented on the array

Skirycz A, Reichelt M, Burow M, Birkemeyer C, Rolcik J, Kopka J, Zanor MI, Gershenzon J, Strnad M, Szopa J, Mueller-Roeber B, Witt I - DOF transcription factor AtDof1

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Gene expression

Northern blot analysis indicated that the OBP2 transcript level increased within 4–6 h upon external MeJA application

Skirycz A, Reichelt M, Burow M, Birkemeyer C, Rolcik J, Kopka J, Zanor MI, Gershenzon J, Strnad M, Szopa J, Mueller-Roeber B, Witt I - DOF transcription factor AtDof1

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Gene expression

To reduce OBP2 transcript level, we transformed Arabidopsis plants with an RNAi–OBP2 construct ... MAM-1 ... not changed

Skirycz A, Reichelt M, Burow M, Birkemeyer C, Rolcik J, Kopka J, Zanor MI, Gershenzon J, Strnad M, Szopa J, Mueller-Roeber B, Witt I - DOF transcription factor AtDof1

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Gene expression

Targeted expression analysis on 35S:OBP2 plants using quantitative real-time PCR (Q-RTPCR) confirmed the increased transcript levels of CYP79B2 ... identified by transcript profiling

Skirycz A, Reichelt M, Burow M, Birkemeyer C, Rolcik J, Kopka J, Zanor MI, Gershenzon J, Strnad M, Szopa J, Mueller-Roeber B, Witt I - DOF transcription factor AtDof1

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Gene expression

Targeted expression analysis on 35S:OBP2 plants using quantitative real-time PCR (Q-RTPCR) confirmed the increased transcript levels of CYP79 ... B3 ... identified by transcript profiling

Skirycz A, Reichelt M, Burow M, Birkemeyer C, Rolcik J, Kopka J, Zanor MI, Gershenzon J, Strnad M, Szopa J, Mueller-Roeber B, Witt I - DOF transcription factor AtDof1

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Gene expression

To reduce OBP2 transcript level, we transformed Arabidopsis plants with an RNAi–OBP2 construct ... ATR1 ... not changed

Skirycz A, Reichelt M, Burow M, Birkemeyer C, Rolcik J, Kopka J, Zanor MI, Gershenzon J, Strnad M, Szopa J, Mueller-Roeber B, Witt I - DOF transcription factor AtDof1

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Gene expression

ARGOS transcripts were highly induced by auxin

Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth

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Gene expression

the induction of ARL by epi-BL was ... still detectable in the BR-deficient mutant det2-1

Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth

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Gene expression

ARL expression was detected ... not in leaf primordia

Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth

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Gene expression

ARL expression was ... also observed in expanding leaves

Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth

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Gene expression

the intracellular localization of ARL by transient expression of a 35S-ARL–GFP or a 35S-GFP–ARL fusion gene in onion epidermal cells using green fluorescent protein (GFP) alone as a control. The intracellular distribution of both fusion proteins was similar to that of GFP alone (Figure 1c), indicating no specific cellular localization of ARL to any cellular compartment

Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth

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Gene expression

TCH4 gene is expressed in expanding cells ... and is responsive to some plant hormones and stimuli, including auxin, BR and touch

Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth

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Gene expression

ARL transcript were ... somewhat repressed by ... GA treatments

Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth

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Gene expression

ARL transcript were just slightly elevated by ... cytokinin

Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth

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Gene expression

ARL transcript were just slightly elevated by auxin

Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth

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Gene expression

ARL transcript were ... somewhat repressed by ABA

Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth

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Gene expression

ARL expression was detected throughout the cotyledons

Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth

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Gene expression

an apparent induction of ARL (2.2-fold) was found when seedlings were treated with epi-brassinolide (epi-BL)

Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth

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Gene expression

ARGOS transcripts were highly induced by ... cytokinin

Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth

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Gene expression

transcript levels of TCH4 were reduced in ARLi lines

Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth

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Gene expression

ARGOS transcripts ... remained almost unchanged under epi-BL treatment

Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth

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Gene expression

ARL expression was detected ... not in ... juvenile leaves

Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth

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Gene expression

transcript levels of TCH4 were ... elevated in ARL-OE lines

Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth

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Gene expression

the induction of ARL by epi-BL was disrupted in bri1–119

Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth

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Gene expression

ARGOS observed only in juvenile leaves

Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth

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Gene expression

ARGOS observed ... in ... vascular tissues of cotyledons

Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth

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Gene expression

Dividing, but not mature, leaf cells contained high SGB1 levels

Wang HX, Weerasinghe RR, Perdue TD, Cakmakci NG, Taylor JP, Marzluff WF, Jones AM - A Golgi-localized hexose transporter is involved in heterotrimeric G protein-mediated early development in Arabidopsis

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Gene expression

Application of d-glucose greatly increased the steady-state level of the SGB1-GUS fusion protein and its distribution pattern

Wang HX, Weerasinghe RR, Perdue TD, Cakmakci NG, Taylor JP, Marzluff WF, Jones AM - A Golgi-localized hexose transporter is involved in heterotrimeric G protein-mediated early development in Arabidopsis

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Gene expression

SGB1 RNA was detected in various tissues of wild-type plants by RT-PCR

Wang HX, Weerasinghe RR, Perdue TD, Cakmakci NG, Taylor JP, Marzluff WF, Jones AM - A Golgi-localized hexose transporter is involved in heterotrimeric G protein-mediated early development in Arabidopsis

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Gene expression

Neither the absence ... of SGB1 had an effect on AGB1 gene expression

Wang HX, Weerasinghe RR, Perdue TD, Cakmakci NG, Taylor JP, Marzluff WF, Jones AM - A Golgi-localized hexose transporter is involved in heterotrimeric G protein-mediated early development in Arabidopsis

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Gene expression

A specific set of newly formed cells of vascular traces in expanding leaves was shown to contain SGB1

Wang HX, Weerasinghe RR, Perdue TD, Cakmakci NG, Taylor JP, Marzluff WF, Jones AM - A Golgi-localized hexose transporter is involved in heterotrimeric G protein-mediated early development in Arabidopsis

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Gene expression

Similarly, sucrose increased the steady-state level of SGB1-GUS but with less efficacy. Sorbitol, inositol, and fructose did not alter the levels or distribution pattern of SGB1 over the no-sugar control

Wang HX, Weerasinghe RR, Perdue TD, Cakmakci NG, Taylor JP, Marzluff WF, Jones AM - A Golgi-localized hexose transporter is involved in heterotrimeric G protein-mediated early development in Arabidopsis

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Gene expression

SGB1 protein was also high in ... embryos

Wang HX, Weerasinghe RR, Perdue TD, Cakmakci NG, Taylor JP, Marzluff WF, Jones AM - A Golgi-localized hexose transporter is involved in heterotrimeric G protein-mediated early development in Arabidopsis

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Gene expression

SGB1-YFP driven by the native promoter in wild-type (Figure 7B) and agb1-2 null (Figure 7C) backgrounds showed that fluorescence was observed in root tissues where the SGB1 promoter is active (Figure 6, B and C). Moreover, the fluorescence imaging was observed to be in multiple punctate compartments within each cell, reminiscent of the size, distribution, and number of Golgi apparatus in plant cells (Boevink et al., 1998 blue right-pointing triangle). Moreover, the dynamics of SGB1-GFP movement (our unpublished data) also matched published Golgi dynamics ... To confirm that the observed fluorescence was the Golgi apparatus, BFA was used. BFA has been shown in plants to disrupt the integrity of Golgi stacks, resulting in the fusion of the endoplasmic reticulum (ER) and the Golgi stacks and the formation of so called “BFA compartments” (Ritzenthaler et al., 2002 blue right-pointing triangle). After treatment with brefeldin-A (Figure 7D, bottom), the GFP fluorescence was observed to change from punctate structures representing the Golgi to both a diffuse fluorescence representing the fused ER/Golgi compartments and larger punctate structures representing the newly formed BFA compartments (Ritzenthaler et al., 2002 blue right-pointing triangle). Z-stack reconstruction imaging of the young leaf epidermal cells of SGB1-GFP plants before (Figure 7E, untreated) and after BFA treatment (Figure 7D, +100 μM BFA) clearly demonstrate the effect of BFA on SGB1 localization. Higher magnification imaging of an individual BFA compartment (Figure 7, F and G) demonstrated that the larger BFA compartments were made up of fused smaller compartments, presumably individual Golgi stacks. Figure 7F is a three-dimensional reconstruction from a Z-stack acquisition of control and BFA-treated cells

Wang HX, Weerasinghe RR, Perdue TD, Cakmakci NG, Taylor JP, Marzluff WF, Jones AM - A Golgi-localized hexose transporter is involved in heterotrimeric G protein-mediated early development in Arabidopsis

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Gene expression

nor excessive amount of SGB1 had an effect on AGB1 gene expression

Wang HX, Weerasinghe RR, Perdue TD, Cakmakci NG, Taylor JP, Marzluff WF, Jones AM - A Golgi-localized hexose transporter is involved in heterotrimeric G protein-mediated early development in Arabidopsis

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Gene expression

RE expression ... in the wild types ... roots, rosette leaves, stems, flower buds, and open flowers of Col-0, En-2 and Ler

González-Bayón R, Kinsman EA, Quesada V, Vera A, Robles P, Ponce MR, Pyke KA, Micol JL - Mutations in the RETICULATA gene dramatically alter internal architecture but have little effect on overall organ shape in Arabidopsis leaves

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Gene expression

RE expression ... GUS activity was also detected in stipules

González-Bayón R, Kinsman EA, Quesada V, Vera A, Robles P, Ponce MR, Pyke KA, Micol JL - Mutations in the RETICULATA gene dramatically alter internal architecture but have little effect on overall organ shape in Arabidopsis leaves

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Gene expression

RE expression ... GUS activity was also detected in ... cauline leaves

González-Bayón R, Kinsman EA, Quesada V, Vera A, Robles P, Ponce MR, Pyke KA, Micol JL - Mutations in the RETICULATA gene dramatically alter internal architecture but have little effect on overall organ shape in Arabidopsis leaves

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Gene expression

RE expression ... GUS activity ... decreased as leaves aged

González-Bayón R, Kinsman EA, Quesada V, Vera A, Robles P, Ponce MR, Pyke KA, Micol JL - Mutations in the RETICULATA gene dramatically alter internal architecture but have little effect on overall organ shape in Arabidopsis leaves

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Gene expression

RE expression ... GUS activity was high in the vasculature of developing leaf primordia

González-Bayón R, Kinsman EA, Quesada V, Vera A, Robles P, Ponce MR, Pyke KA, Micol JL - Mutations in the RETICULATA gene dramatically alter internal architecture but have little effect on overall organ shape in Arabidopsis leaves

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Gene expression

Subsequently, during leaf development, the pattern of PPD expression followed the tip-to-base progression of the general proliferative arrest front before becoming restricted to developing vascular traces and eventually declining in expression in the same pattern as the arrest of procambial cell division

White DW - PEAPOD regulates lamina size and curvature in Arabidopsis

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Gene expression

During leaf development, PPD expression was first detected at the tip of the developing leaf, distal to the general proliferative cell-cycle arrest front and coincident with the initiation of trichome and margin cell development (Fig. 4 A and D Inset). This expression appeared to exist initially in all cells in the newly formed DMC zone and was not restricted to meristemoid cells

White DW - PEAPOD regulates lamina size and curvature in Arabidopsis

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Gene expression

Subsequently, during leaf development, the pattern of PPD expression followed the tip-to-base progression of the general proliferative arrest front before becoming restricted to developing vascular traces and eventually declining in expression in the same pattern as the arrest of procambial cell division

White DW - PEAPOD regulates lamina size and curvature in Arabidopsis

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Gene expression

During leaf development, PPD expression was first detected at the tip of the developing leaf, distal to the general proliferative cell-cycle arrest front and coincident with the initiation of trichome and margin cell development (Fig. 4 A and D Inset). This expression appeared to exist initially in all cells in the newly formed DMC zone and was not restricted to meristemoid cells

White DW - PEAPOD regulates lamina size and curvature in Arabidopsis

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Gene expression

DR5-GUS reporter was not expressed in the veins of 21-day-old Col-0 and hve-3/hve-3 leaves from the first, third and seventh nodes

Alonso-Peral MM, Candela H, del Pozo JC, Martínez-Laborda A, Ponce MR, Micol JL - The HVE/CAND1 gene is required for the early patterning of leaf venation in Arabidopsis

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Gene expression

ATHB-8-GUS ... the expression of the reporter revealed a much simpler GUS pattern in hve-3/hve-3 leaves than in wild-type leaves of the same age (compare Fig. 5A with 5I, 5B with 5J, 5C with 5K, and 5D with 5L). The GUS pattern was restricted to the fully differentiated venation pattern of the mutant, which lacked most tertiary and higher order veins. The absence of GUS staining at the sites where the veins normally differentiate indicates that ATHB-8-GUS expression is dependent on the earlier acquisition of vascular fate at specific locations within the leaf. Identical results were obtained in comparisons of Ws-2 with hve-1/hve-1

Alonso-Peral MM, Candela H, del Pozo JC, Martínez-Laborda A, Ponce MR, Micol JL - The HVE/CAND1 gene is required for the early patterning of leaf venation in Arabidopsis

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Gene expression

Col-0 RNA was extracted from assorted organs and RT-PCR amplified, and HVE was found to be expressed in every organ studied

Alonso-Peral MM, Candela H, del Pozo JC, Martínez-Laborda A, Ponce MR, Micol JL - The HVE/CAND1 gene is required for the early patterning of leaf venation in Arabidopsis

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Gene expression

postembryonic spatial expression pattern ... HVE ... GUS staining was mostly confined to the leaf vasculature in 21-day-old plants, indicating that HVE expression changes dynamically throughout leaf development (Fig. 4C). For cotyledons and vegetative leaves, the expression in mesophyll cells was apparently more intense at the beginning of leaf expansion, disappearing progressively as the leaves grew (Fig. 4D,E-H,P). Closer inspection revealed that the gene was expressed before xylem differentiation in developing veins at the basal actively dividing region of rosette leaves (Fig. 4R) and, after the differentiation of tracheary elements, in the living cells of the vascular bundles (Fig. 4Q

Alonso-Peral MM, Candela H, del Pozo JC, Martínez-Laborda A, Ponce MR, Micol JL - The HVE/CAND1 gene is required for the early patterning of leaf venation in Arabidopsis

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Gene expression

To further define the postembryonic spatial expression pattern of HVE/CAND1, we characterized the wild-type expression pattern (Fig. 4) of a CAND1 promoter-GUS fusion (PETA2-GUS) that includes 2.7 kb of upstream sequence (Chuang et al., 2004). Consistent with the pleiotropic phenotype of loss-of-function hve mutants and with our semi-quantitative RT-PCR results, the gene was found almost ubiquitously expressed in aerial and underground organs of 10-day-old plants (Fig. 4A,B). At this stage, the gene was widely expressed in rosette leaves, at the highest level in the vasculature

Alonso-Peral MM, Candela H, del Pozo JC, Martínez-Laborda A, Ponce MR, Micol JL - The HVE/CAND1 gene is required for the early patterning of leaf venation in Arabidopsis

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Gene expression

DR5-GUS reporter ... was in the leaf margin, the hydathodes and some mesophyll regions. For actively developing leaves of the 9th and upper nodes, by contrast, the DR5-GUS reporter was expressed at the tip, hydathodes, and tertiary and higher order veins of the proximal regions of the lamina in the wild type, but only at the tip in hve-3/hve-3 leaves

Alonso-Peral MM, Candela H, del Pozo JC, Martínez-Laborda A, Ponce MR, Micol JL - The HVE/CAND1 gene is required for the early patterning of leaf venation in Arabidopsis

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Gene expression

ATHB-8-GUS reporter ... in ... wild-type leaves, the expression of the reporter uncovered a complex, reticulate pattern consisting of mature and differentiating vascular strands

Alonso-Peral MM, Candela H, del Pozo JC, Martínez-Laborda A, Ponce MR, Micol JL - The HVE/CAND1 gene is required for the early patterning of leaf venation in Arabidopsis

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Gene expression

We observed a similar expression profile of ectopic BP but not STM in leaves of a dominant Aux/IAA17 mutant, axr3-1

Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis

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Gene expression

polar localisation of PIN1:GFP in margin cells of plants grown on MS medium (arrowheads, Fig. 3G) were abolished in NPA-grown plants

Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis

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Gene expression

In comparison with wild-type leaves ... DR5::GUS expression was repressed in the distal lamina and concentrated in developing lobes of 35S::BP leaves

Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis

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Gene expression

This ectopic BP expression in axr1 leaves was not accompanied by a reduction in AS1 transcripts

Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis

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Gene expression

BP::GUS ... expression was observed in the serration tips of axr1 leaves

Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis

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Gene expression

we observed ectopic BP::GUS expression in the leaves of plants treated with the auxin transport inhibitors 1-N-naphthylphthalamic acid (NPA) or 2,3,5triiodobenzoic acid (TIBA

Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis

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Gene expression

We observed inappropriate expression of the KNOX gene BP but not SHOOTMERISTEMLESS (STM) in axr1 mutant leaves, although the level of BP expression was substantially higher in as1 than in axr1 leaves

Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis

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Gene expression

We observed inappropriate expression of the KNOX gene BP ... in axr1 mutant leaves

Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis

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Gene expression

in FIL>>BP plants ... NPA treatment ... prevented the generation of PIN1-directed auxin maxima in the leaf margin

Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis

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Gene expression

In comparison with wild-type leaves ... PIN1::GUS ... expression was repressed in the distal lamina and concentrated in developing lobes of 35S::BP leaves

Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis

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Gene expression

We observed inappropriate expression of the KNOX gene BP but not SHOOTMERISTEMLESS (STM) in axr1

Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis

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Gene expression

PIN1:GFP expression in initiating leaflets of FIL>>BP leaves was indistinguishable from that of wild type; however, expression persisted as leaflets developed in FIL>>BP leaves (arrows Fig. 4G), suggesting that KNOX activity in the leaf prevents the normal basipetal displacement of PIN1:GFP expression maxima, correlating with prolonged localised growth and leaflet formation

Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis

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Gene expression

BP::GUS was absent from wild-type leaves

Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis

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Gene expression

PIN1:GFP expression in initiating leaflets of FIL>>BP leaves was indistinguishable from that of wild type; however, expression persisted as leaflets developed in FIL>>BP leaves (arrows Fig. 4G), suggesting that KNOX activity in the leaf prevents the normal basipetal displacement of PIN1:GFP expression maxima, correlating with prolonged localised growth and leaflet formation. BP-induced alterations in PIN1:GFP expression were mirrored by similar alterations in expression of the AINTEGUMENTA (ANT) gene

Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis

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Gene expression

DR5rev::GFP was localised in the tips of initiating serrations

Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis

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Gene expression

in pin1 leaves, relative to wild type ... AS1 transcript levels were unaltered

Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis

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Gene expression

BP::GUS ... was observed ... in the ... midvein ... of as1 leaves

Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis

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Gene expression

We observed inappropriate expression of the KNOX gene BP ... expression was substantially higher in as1 than in axr1 leaves

Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis

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Gene expression

We observed a similar expression profile of ectopic BP ... in leaves of a dominant Aux/IAA17 mutant, axr3-1

Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis

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Gene expression

polar expression of PIN1:GFP in the epidermis

Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis

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Gene expression

BP::GUS ... expression was observed ... in the petiole, midvein and serration tips of as1 leaves

Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis

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Gene expression

in the leaves of plants treated with the auxin transport inhibitors 1-N-naphthylphthalamic acid (NPA) or 2,3,5triiodobenzoic acid (TIBA ... STM::GUS expression was unaltered

Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis

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Gene expression

BP::GUS ... expression was observed ... in the petiole ... of as1 leaves

Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis

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Gene expression

We observed misexpression of BP transcripts in pin1 leaves, relative to wild type

Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis

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Gene expression

in as1 ... leaves ... This ectopic BP expression ... was not accompanied by a reduction in AS1 transcripts

Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis

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Gene expression

Although BP::GUS was absent from wild-type leaves (Fig. 1R), expression was observed in the serration tips of axr1 leaves (Fig. 1S), and in the petiole, midvein and serration tips of as1 leaves (Fig. 1T). However, the pattern of BP::GUS expression in as1;axr1 double mutant leaves was different than that of either single mutant, being sharply localised to margin cells in the sinus of every lobe from an early stage in leaf development (arrows, Fig. 1U,V), correlating with the ectopic initiation of stipules

Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis

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Gene expression

FAS1 transcript ... fas1-1 ... is a null allele

Exner V, Taranto P, Schönrock N, Gruissem W, Hennig L - Chromatin assembly factor CAF-1 is required for cellular differentiation during plant development

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Gene expression

some FAS1 transcript is made in the fas1-4 insertion mutant (see Fig. S1 in the supplementary material), demonstrating that fas1-1 but not fas1-4 is a null allele

Exner V, Taranto P, Schönrock N, Gruissem W, Hennig L - Chromatin assembly factor CAF-1 is required for cellular differentiation during plant development

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Gene expression

fas2 alleles appear to be null

Exner V, Taranto P, Schönrock N, Gruissem W, Hennig L - Chromatin assembly factor CAF-1 is required for cellular differentiation during plant development

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Gene expression

the Atebp1(RNAi) plants had a ... reduction of the CDKB1;1

Horváth BM, Magyar Z, Zhang Y, Hamburger AW, Bakó L, Visser RG, Bachem CW, Bögre L - EBP1 regulates organ size through cell growth and proliferation in plants

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Gene expression

We found that the overexpression of EBP1 in Arabidopsis cells dose-dependently reduced the endogenous RBR1 protein

Horváth BM, Magyar Z, Zhang Y, Hamburger AW, Bakó L, Visser RG, Bachem CW, Bögre L - EBP1 regulates organ size through cell growth and proliferation in plants

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Gene expression

We found that silencing of AtEBP1 completely abolished the activation of RNR2 promoter by auxin (Figure 5E), indicating that AtEBP1 is required for the auxin-dependent promoter activity

Horváth BM, Magyar Z, Zhang Y, Hamburger AW, Bakó L, Visser RG, Bachem CW, Bögre L - EBP1 regulates organ size through cell growth and proliferation in plants

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Gene expression

In contrast, the Atebp1(RNAi) plants had a consistently elevated RBR1 protein accumulation

Horváth BM, Magyar Z, Zhang Y, Hamburger AW, Bakó L, Visser RG, Bachem CW, Bögre L - EBP1 regulates organ size through cell growth and proliferation in plants

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Gene expression

expression levels of ... CYP90D1 (a ROT3 homolog ... not altered in ... lng1-3 lng2-1

Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis

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Gene expression

we determined the expression patterns of these genes in promoter:GUS transgenic plants. Promoter sequences (∼2 kb) from the LNG1 and LNG2 genes were used to drive expression of GUS. The ProLNG1:GUS ... expressed in ... leaf blades

Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis

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Gene expression

we determined the expression patterns of these genes in promoter:GUS transgenic plants. Promoter sequences (∼2 kb) from the LNG1 and LNG2 genes were used to drive expression of GUS. The ProLNG1:GUS ... expressed in various parts of adult transgenic plants, including the petioles

Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis

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Gene expression

we determined the expression patterns of these genes in promoter:GUS transgenic plants. Promoter sequences (∼2 kb) from the LNG1 and LNG2 genes were used to drive expression of GUS ... ProLNG2:GUS ... expressed in ... petioles

Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis

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Gene expression

lng1-1D ... T-DNA insertion at 5477 bp upstream of At5g15880 ... Northern blot analysis indicated that the expression of one gene (At5g15880) was higher in mutant plants compared with wild type

Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis

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Gene expression

expression levels of ... AN ... not altered in lng1-1D

Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis

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Gene expression

LNG2 expression levels were unchanged in the rot3-1 mutant

Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis

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Gene expression

ProLNG1:GUS ... GUS staining ... stronger staining in the veins

Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis

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Gene expression

expression levels of ... ATHB13 were not altered in lng1-1D

Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis

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Gene expression

ProLNG2:GUS ... In leaves, GUS staining was observed in all parts

Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis

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Gene expression

expression levels of ROT3 ... not altered in ... lng1-3 lng2-1

Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis

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Gene expression

ProLNG2:GUS ... GUS was strongly expressed in and at the base of the petioles

Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis

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Gene expression

ProLNG1:GUS ... GUS was strongly expressed in and at the base of the petioles

Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis

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Gene expression

expression levels of ROT3 ... not altered in lng1-1D

Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis

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Gene expression

ProLNG1:GUS ... In leaves, GUS staining was observed in all parts

Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis

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Gene expression

The GFP-LNG1 fusion protein showed similar localizations in ... nucleus

Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis

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Gene expression

expression levels of ... AN ... not altered in ... lng1-3 lng2-1

Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis

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Gene expression

expression levels of ... ATHB13 were not altered in ... lng1-3 lng2-1

Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis

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Gene expression

expression levels of ... CYP90D1 (a ROT3 homolog ... not altered in lng1-1D

Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis

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Gene expression

The GFP-LNG1 fusion protein showed similar localizations in ... the cytosol

Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis

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Gene expression

LNG1 ... expression levels were unchanged in the rot3-1 mutant

Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis

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Gene expression

we determined the expression patterns of these genes in promoter:GUS transgenic plants. Promoter sequences (∼2 kb) from the LNG1 and LNG2 genes were used to drive expression of GUS ... ProLNG2:GUS ... expressed in ... leaf blades

Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis

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Gene expression

ProLNG2:GUS ... GUS staining ... stronger staining in the veins

Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis

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Gene expression

To obtain further insights into the potential role of farnesylation for AtNAP1;1 subcellular localization, GFP-AtNAP1;1 and GFP-AtNAP1;1C369S were stably expressed in transgenic tobacco BY-2 cells (Fig. 2B). GFP-AtNAP1;1 was localized in both the cytoplasm and the nucleoplasm of interphase cells. During mitosis, GFP-AtNAP1;1 was colocalized with the phragmoplast in telophase

Galichet A, Gruissem W - Developmentally controlled farnesylation modulates AtNAP1;1 function in cell proliferation and cell expansion during Arabidopsis leaf development

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Gene expression

To investigate the subcellular localization of AtNAP1;1, GFP-AtNAP1;1 and GFP-AtNAP1;1C369S were transiently expressed in onion (Allium cepa) epidermal cells. GFP alone was distributed uniformly in the nucleus and the cytoplasm, whereas GFP-AtNAP1;1 and GFP-AtNAP1;1C369S were restricted to the cytoplasm

Galichet A, Gruissem W - Developmentally controlled farnesylation modulates AtNAP1;1 function in cell proliferation and cell expansion during Arabidopsis leaf development

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Gene expression

BOLpromoter-GUS construct was used to study further the temporal and spatial pattern of expression ... Five days after germination, seedlings showed mild staining at the SAM

Marsch-Martinez N, Greco R, Becker JD, Dixit S, Bergervoet JH, Karaba A, de Folter S, Pereira A - BOLITA, an Arabidopsis AP2/ERF-like transcription factor that affects cell expansion and proliferation/differentiation pathways

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Gene expression

bol- D ... Arabidopsis leaves ... many calcium or calmodulin binding genes, including the calcium dependent protein kinase CPK32, were also downregulated

Marsch-Martinez N, Greco R, Becker JD, Dixit S, Bergervoet JH, Karaba A, de Folter S, Pereira A - BOLITA, an Arabidopsis AP2/ERF-like transcription factor that affects cell expansion and proliferation/differentiation pathways

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Gene expression

bol- D ... Arabidopsis leaves ... The auxin induced genes TCH3 and PBP1 which contain calcium binding motives and interact in a calcium dependent manner with the PINOID kinase, a key component in auxin signaling, were also repressed

Marsch-Martinez N, Greco R, Becker JD, Dixit S, Bergervoet JH, Karaba A, de Folter S, Pereira A - BOLITA, an Arabidopsis AP2/ERF-like transcription factor that affects cell expansion and proliferation/differentiation pathways

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Gene expression

BOLpromoter-GUS construct was used to study further the temporal and spatial pattern of expression ... In the first 2 days after germination, staining occurred at the shoot apical meristem

Marsch-Martinez N, Greco R, Becker JD, Dixit S, Bergervoet JH, Karaba A, de Folter S, Pereira A - BOLITA, an Arabidopsis AP2/ERF-like transcription factor that affects cell expansion and proliferation/differentiation pathways

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Gene expression

BOLpromoter-GUS construct was used to study further the temporal and spatial pattern of expression ... Emerging leaves from older seedlings stained first at the tip and later at separated spots at the leaf periphery (hydatodes)

Marsch-Martinez N, Greco R, Becker JD, Dixit S, Bergervoet JH, Karaba A, de Folter S, Pereira A - BOLITA, an Arabidopsis AP2/ERF-like transcription factor that affects cell expansion and proliferation/differentiation pathways

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Gene expression

BOLpromoter-GUS construct was used to study further the temporal and spatial pattern of expression ... Five days after germination, seedlings showed ... intense staining at leaf primordia

Marsch-Martinez N, Greco R, Becker JD, Dixit S, Bergervoet JH, Karaba A, de Folter S, Pereira A - BOLITA, an Arabidopsis AP2/ERF-like transcription factor that affects cell expansion and proliferation/differentiation pathways

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Gene expression

BOLpromoter-GUS construct was used to study further the temporal and spatial pattern of expression ... In the first 2 days after germination, staining occurred ... at the distal regions of the cotyledons

Marsch-Martinez N, Greco R, Becker JD, Dixit S, Bergervoet JH, Karaba A, de Folter S, Pereira A - BOLITA, an Arabidopsis AP2/ERF-like transcription factor that affects cell expansion and proliferation/differentiation pathways

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Gene expression

RT-PCR experiments were then performed to assess expression of AP2/ERF gene At1g24590, representing the best candidate based on the position of the insert in the activation tag mutant and the nature of the gene itself. While in wild type plants the presence of its transcript was detected only in flower buds, in the bol-D mutant hyper-accumulation of this transcript occurred in roots, rosette and cauline leaves, flowers buds and open flowers

Marsch-Martinez N, Greco R, Becker JD, Dixit S, Bergervoet JH, Karaba A, de Folter S, Pereira A - BOLITA, an Arabidopsis AP2/ERF-like transcription factor that affects cell expansion and proliferation/differentiation pathways

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Gene expression

bol- D ... Arabidopsis leaves ... The auxin induced genes TCH3 and PBP1 which contain calcium binding motives and interact in a calcium dependent manner with the PINOID kinase, a key component in auxin signaling, were also repressed

Marsch-Martinez N, Greco R, Becker JD, Dixit S, Bergervoet JH, Karaba A, de Folter S, Pereira A - BOLITA, an Arabidopsis AP2/ERF-like transcription factor that affects cell expansion and proliferation/differentiation pathways

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Gene expression

ProCUC2:GUS reporter ... In larger leaves, GUS staining was restricted to the proximal part of the leaves, in which the teeth were still forming

Nikovics K, Blein T, Peaucelle A, Ishida T, Morin H, Aida M, Laufs P - The balance between the MIR164A and CUC2 genes controls leaf margin serration in Arabidopsis

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Gene expression

ProMIR164A-2.1:GUS construct ... later appeared ... in the vasculature

Nikovics K, Blein T, Peaucelle A, Ishida T, Morin H, Aida M, Laufs P - The balance between the MIR164A and CUC2 genes controls leaf margin serration in Arabidopsis

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Gene expression

GUS staining was observed at the point of insertion of the primordium into the meristem in plants carrying the ProMIR164A-2.1:GUS construct

Nikovics K, Blein T, Peaucelle A, Ishida T, Morin H, Aida M, Laufs P - The balance between the MIR164A and CUC2 genes controls leaf margin serration in Arabidopsis

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Gene expression

In mir164a-4 leaves ... At5g61430 transcript levels were two to three times higher than those in the wild type

Nikovics K, Blein T, Peaucelle A, Ishida T, Morin H, Aida M, Laufs P - The balance between the MIR164A and CUC2 genes controls leaf margin serration in Arabidopsis

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Gene expression

no ectopic KNAT1 ... expression was observed in the serrated CUC2g-m4 leaves

Nikovics K, Blein T, Peaucelle A, Ishida T, Morin H, Aida M, Laufs P - The balance between the MIR164A and CUC2 genes controls leaf margin serration in Arabidopsis

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Gene expression

In mir164a-4 leaves ... At5g07680 ... transcript levels were two to three times higher than those in the wild type

Nikovics K, Blein T, Peaucelle A, Ishida T, Morin H, Aida M, Laufs P - The balance between the MIR164A and CUC2 genes controls leaf margin serration in Arabidopsis

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Gene expression

In mir164a-4 leaves ... CUC2 transcript levels were about five times higher than those in the wild type

Nikovics K, Blein T, Peaucelle A, Ishida T, Morin H, Aida M, Laufs P - The balance between the MIR164A and CUC2 genes controls leaf margin serration in Arabidopsis

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Gene expression

no ectopic ... KNAT2 expression was observed in the serrated CUC2g-m4 leaves

Nikovics K, Blein T, Peaucelle A, Ishida T, Morin H, Aida M, Laufs P - The balance between the MIR164A and CUC2 genes controls leaf margin serration in Arabidopsis

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Gene expression

ProMIR164A-2.1:GUS ... later appeared at the tip of the organ

Nikovics K, Blein T, Peaucelle A, Ishida T, Morin H, Aida M, Laufs P - The balance between the MIR164A and CUC2 genes controls leaf margin serration in Arabidopsis

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Gene expression

ProMIR164A-2.1:GUS construct ... In small primordia, GUS activity was observed at the margins

Nikovics K, Blein T, Peaucelle A, Ishida T, Morin H, Aida M, Laufs P - The balance between the MIR164A and CUC2 genes controls leaf margin serration in Arabidopsis

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Gene expression

ProCUC2:GUS reporter ... During leaf development, GUS activity was observed in the margins of the developing primordium

Nikovics K, Blein T, Peaucelle A, Ishida T, Morin H, Aida M, Laufs P - The balance between the MIR164A and CUC2 genes controls leaf margin serration in Arabidopsis

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Gene expression

In mir164a-4 leaves, NAC1 ... transcript levels ... were two to three times higher than those in the wild type

Nikovics K, Blein T, Peaucelle A, Ishida T, Morin H, Aida M, Laufs P - The balance between the MIR164A and CUC2 genes controls leaf margin serration in Arabidopsis

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Gene expression

A transcriptional reporter for MUTE is expressed ... at lower levels in ... guard cells

MacAlister CA, Ohashi-Ito K, Bergmann DC - Transcription factor control of asymmetric cell divisions that establish the stomatal lineage

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Gene expression

A transcriptional green fluorescent protein (GFP) reporter (SPCHpro::nucGFP) and a rescuing translational reporter (SPCHpro::SPCH�GFP) are expressed in a subset of epidermal cells that lack overt signs of differentiation ... Expression commences in cotyledons coincident with the onset of postembryonic cell division

MacAlister CA, Ohashi-Ito K, Bergmann DC - Transcription factor control of asymmetric cell divisions that establish the stomatal lineage

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Gene expression

SPCH is expressed in the developing leaf epidermis

MacAlister CA, Ohashi-Ito K, Bergmann DC - Transcription factor control of asymmetric cell divisions that establish the stomatal lineage

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Gene expression

spch-1 plants also fail to express TMM

MacAlister CA, Ohashi-Ito K, Bergmann DC - Transcription factor control of asymmetric cell divisions that establish the stomatal lineage

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Gene expression

In older organs, SPCHpro::SPCH�GFP expression continues to be restricted to small cells in the epidermis (Fig. 2d), including cells that have recently divided next to stomatal lineage cells

MacAlister CA, Ohashi-Ito K, Bergmann DC - Transcription factor control of asymmetric cell divisions that establish the stomatal lineage

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Gene expression

SPCHpro::nucGFP persists in stomatal lineage cells

MacAlister CA, Ohashi-Ito K, Bergmann DC - Transcription factor control of asymmetric cell divisions that establish the stomatal lineage

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Gene expression

A transcriptional reporter for MUTE is expressed ... at lower levels in GMCs

MacAlister CA, Ohashi-Ito K, Bergmann DC - Transcription factor control of asymmetric cell divisions that establish the stomatal lineage

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Gene expression

SPCH expression is often found in two neighbouring cells�a pattern consistent with expression in the dividing cell population

MacAlister CA, Ohashi-Ito K, Bergmann DC - Transcription factor control of asymmetric cell divisions that establish the stomatal lineage

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Gene expression

A transcriptional reporter for MUTE is expressed strongly in meristemoids

MacAlister CA, Ohashi-Ito K, Bergmann DC - Transcription factor control of asymmetric cell divisions that establish the stomatal lineage

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Gene expression

FAMA is expressed in ... immature guard cells

Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata

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Gene expression

To examine ... subcellular localization, we used ... the translational fusion of a full-length MUTE protein and GFP (MUTE ... MUTE�GFP ... No GFP signal was detected in ... newly formed meristemoids

Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata

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Gene expression

SPCH promoter activity (SPCH::GUS) was observed broadly in the protoderm of leaf primordia

Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata

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Gene expression

The ... arrested mute meristemoids expressed high levels ... ERL1::GUS, a reporter b-glucuronidase driven by the ERL1 promoter6

Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata

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Gene expression

The arrested mute meristemoids expressed high levels of TMM::TMM�GFP, a translational fusion of TMM with a green fluorescent protein

Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata

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Gene expression

Bubble-like� clusters of small cells ... observed in the epidermis of ... mute; erecta; erl1; erl2 ... expressed TMM::GUS ... at high levels

Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata

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Gene expression

To examine promoter activity ... we used the native MUTE promoter to drive expression of the GUS reporter (MUTE::GUS ... MUTE::GUS expression ... residual activity in ... immature guard cells

Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata

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Gene expression

In ... ectopic overexpression of SPCH by the CaMV35S promoter ... numerous small cells ... strongly express TMM::TMM�GFP, indicating that they probably have the identity of stomatal lineage cells

Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata

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Gene expression

ERL1 mark stomatal lineage cells ... ERL1::GUS, a reporter b-glucuronidase driven by the ERL1 promoter6

Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata

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Gene expression

Likewise, the arrested meristemoids in mute did not express the mature-guard-cell-specific GFP marker E994

Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata

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Gene expression

TMM ... mark stomatal lineage cells with the highest expression in meristemoids ... TMM::TMM�GFP

Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata

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Gene expression

To examine promoter activity ... we used the native MUTE promoter to drive expression of the GUS reporter (MUTE::GUS ... MUTE::GUS expression was highest in a subset of meristemoids that have undergone a few rounds of asymmetric division

Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata

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Gene expression

In the wild-type leaf epidermis, FAMA�GFP was ... not in meristemoids

Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata

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Gene expression

FAMA::FAMA�GFP, a translational fusion of GFP and FAMA ... In the wild-type leaf epidermis, FAMA�GFP was specifically detected in the nuclei of GMCs

Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata

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Gene expression

To examine ... subcellular localization, we used ... the translational fusion of a full-length MUTE protein and GFP (MUTE::MUTE�GFP ... The MUTE�GFP fusion protein was localized in the nuclei ... nuclei of ... recently transitioned GMCs

Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata

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Gene expression

Bubble-like� clusters of small cells ... observed in the epidermis of ... mute; erecta; erl1; erl2 ... expressed ... ERL1::GUS at high levels

Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata

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Gene expression

In the wild-type leaf epidermis, FAMA�GFP was specifically detected in the nuclei ... early, immature guard cells

Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata

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Gene expression

To examine ... subcellular localization, we used ... the translational fusion of a full-length MUTE protein and GFP (MUTE::MUTE�GFP ... No GFP signal was detected in ... immature ... e and mature guard cells

Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata

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Gene expression

FAMA is expressed in GMCs

Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata

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Gene expression

Bubble-like� clusters of small cells ... observed in the epidermis of mute; tmm ... expressed ... ERL1::GUS at high levels

Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata

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Gene expression

In mute, no FAMA�GFP expression was detected

Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata

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Gene expression

Bubble-like� clusters of small cells ... observed in the epidermis of mute; tmm ... expressed TMM::GUS ... at high levels

Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata

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Gene expression

TMM ... mark stomatal lineage cells with the highest expression in meristemoids ... TMM::TMM�GFP

Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata

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Gene expression

To examine ... subcellular localization, we used ... the translational fusion of a full-length MUTE protein and GFP (MUTE::MUTE�GFP ... The MUTE�GFP fusion protein was localized in the nuclei of a subset of meristemoids that have undergone a few rounds of asymmetric division

Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata

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Gene expression

SPCH promoter activity (SPCH::GUS) was ... localized in stomatal lineage cells later in development

Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata

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Gene expression

The spch mutant does not express MUTE transcripts

Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata

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Gene expression

To examine promoter activity ... we used the native MUTE promoter to drive expression of the GUS reporter ... residual activity in GMC

Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata

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Gene expression

ERL1 mark stomatal lineage cells with the highest expression in meristemoids ... ERL1::GUS, a reporter b-glucuronidase driven by the ERL1 promoter6

Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata

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Gene expression

During leaf development ... expression of ... AtML1∷KRP4 was observed ... in the abaxial epidermis

Bemis SM, Torii KU - Autonomy of cell proliferation and developmental programs during Arabidopsis aboveground organ morphogenesis

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Gene expression

no difference was observed in CLV3 ... expression levels ... between transgenic and Ler plants

Bemis SM, Torii KU - Autonomy of cell proliferation and developmental programs during Arabidopsis aboveground organ morphogenesis

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Gene expression

no difference was observed in CLV3 expression ... patterns between transgenic and Ler plants

Bemis SM, Torii KU - Autonomy of cell proliferation and developmental programs during Arabidopsis aboveground organ morphogenesis

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Gene expression

We ... investigated expression patterns in the transgenic plants expressing ... AtML1∷KRP4 ... L1-layer-specific accumulation of ... KRP4 transcripts was detected in the ... leaf primordia

Bemis SM, Torii KU - Autonomy of cell proliferation and developmental programs during Arabidopsis aboveground organ morphogenesis

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Gene expression

We ... investigated expression patterns in the transgenic plants expressing ... AtML1∷KRP4 ... L1-layer-specific accumulation of ... KRP4 transcripts was detected in the SAM

Bemis SM, Torii KU - Autonomy of cell proliferation and developmental programs during Arabidopsis aboveground organ morphogenesis

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Gene expression

We ... investigated expression patterns in the transgenic plants expressing AtML1∷KRP1/ICK1 ... L1-layer-specific accumulation of KRP1/ICK1 ... transcripts was detected in the SAM

Bemis SM, Torii KU - Autonomy of cell proliferation and developmental programs during Arabidopsis aboveground organ morphogenesis

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Gene expression

no difference was observed in CLV3 expression ... patterns between transgenic and Ler plants

Bemis SM, Torii KU - Autonomy of cell proliferation and developmental programs during Arabidopsis aboveground organ morphogenesis

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Gene expression

We ... investigated expression patterns in the transgenic plants expressing AtML1∷KRP1/ICK1 ... L1-layer-specific accumulation of KRP1/ICK1 ... transcripts was detected in the ... leaf primordia

Bemis SM, Torii KU - Autonomy of cell proliferation and developmental programs during Arabidopsis aboveground organ morphogenesis

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Gene expression

expression patterns of endogenous ... KRP4 transcripts were examined in non-transformed Ler plants ... KRP4 was detected in the SAM

Bemis SM, Torii KU - Autonomy of cell proliferation and developmental programs during Arabidopsis aboveground organ morphogenesis

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Gene expression

expression patterns of endogenous KRP1/ICK1 ... transcripts were examined in non-transformed Ler plants. We did not detect significant expression of KRP1/ICK1 beyond the level of the sense control, perhaps due to its uniform expression in vacuolated, mature cells

Bemis SM, Torii KU - Autonomy of cell proliferation and developmental programs during Arabidopsis aboveground organ morphogenesis

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Gene expression

AtML1∷KRP4 transgenic lines, cells in the inner layers did not exhibit high accumulation of ... KRP4 beyond the levels of endogenous transcripts

Bemis SM, Torii KU - Autonomy of cell proliferation and developmental programs during Arabidopsis aboveground organ morphogenesis

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Gene expression

During leaf development ... expression of AtML1∷KRP1/ICK1 ... was observed in the adaxial epidermis

Bemis SM, Torii KU - Autonomy of cell proliferation and developmental programs during Arabidopsis aboveground organ morphogenesis

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Gene expression

AtML1∷KRP1/ICK1 ... transgenic lines, cells in the inner layers did not exhibit high accumulation of KRP1/ICK1 ... beyond the levels of endogenous transcripts

Bemis SM, Torii KU - Autonomy of cell proliferation and developmental programs during Arabidopsis aboveground organ morphogenesis

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Gene expression

During leaf development ... expression of AtML1∷KRP1/ICK1 ... was observed ... in the abaxial epidermis

Bemis SM, Torii KU - Autonomy of cell proliferation and developmental programs during Arabidopsis aboveground organ morphogenesis

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Gene expression

During leaf development ... expression of ... AtML1∷KRP4 was observed in the adaxial epidermis

Bemis SM, Torii KU - Autonomy of cell proliferation and developmental programs during Arabidopsis aboveground organ morphogenesis

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Gene expression

no difference was observed in CLV3 expression levels ... between transgenic and Ler plants

Bemis SM, Torii KU - Autonomy of cell proliferation and developmental programs during Arabidopsis aboveground organ morphogenesis

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Gene expression

Two upregulated genes were involved in callose synthesis during cell plate formation: ENDOXYLOGLUCAN TRANSFERASE (DECOY) and CALLOSE SYNTHASE1, both of which are implicated in cell wall/cell plate formation (Table 3 ... in hub1-1

Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth

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Gene expression

Other categories downregulated in hub1-1 comprised genes involved in carboxylic acid metabolism, fatty acid metabolism, lipid biosynthesis, vitamin biosynthesis, cofactor biosynthesis, chlorophyll biosynthesis, transfer RNA metabolism, photosynthesis, and protein targeted to chloroplast

Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth

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Gene expression

the E2F dimerization partner (DPa) transcription factor gene were upregulated ... in hub1-1

Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth

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Gene expression

some overex- pressed genes in hub1-1 were related to meristem development, such as the homeodomain genes ... SHOOT MERISTEMLESS

Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth

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Gene expression

the analysis of upregulated genes also identified GO categories related to cytokinesis ... in hub1-1

Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth

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Gene expression

A significantly high number of genes differentially ex- pressed in hub1-1 were related to cell cycle (24 genes) and cytokinesis (31 genes)

Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth

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Gene expression

Among the downregulated genes in hub1-1, cell fate specifi- cation, histone phosphorylation, regulation of progression through cell cycle, mitotic cell cycle, and microtubule-based movement genes were significantly overrepresented. For cellular compo- nents, the underexpressed genes in hub1-1 were significantly related to myosin, spindle, microtubule, and phragmoplast (data not shown). Among the 82 genes with a peak expression in mitosis (Menges et al., 2005), 66 were significantly downregulated in the hub1-1 mutant, of which 37 genes had a known function in mitosis

Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth

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Gene expression

Two upregulated genes were involved in callose synthesis during cell plate formation: ENDOXYLOGLUCAN TRANSFERASE (DECOY) and CALLOSE SYNTHASE1, both of which are implicated in cell wall/cell plate formation (Table 3 ... in hub1-1

Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth

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Gene expression

the SIAMESE (SIM) gene, with a function in endoreduplication during trichome formation on leaves ... upregulated ... in hub1-1

Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth

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Gene expression

some overex- pressed genes in hub1-1 were related to meristem development, such as the homeodomain genes ... KNAT6

Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth

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Gene expression

A total of 13 homeotic genes had an altered expression in hub1-1, among which were two SNF2 pro- teins (At3g63950 and At1g05480)

Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth

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Gene expression

some overex- pressed genes in hub1-1 were related to meristem development, such as the homeodomain genes KNOTTED1-LIKE2 (KNAT2)

Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth

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Gene expression

Of the cytokinesis-related genes, 26 kinesins or kinesin-like genes and genes related to microtubule and myosin were downregulated in hub1-1, among which are the kinesins ... TETRASPORE (NACK2)

Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth

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Gene expression

some overex- pressed genes in hub1-1 were related to meristem development, such as ... 12 NO APICAL MERI- STEM genes

Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth

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Gene expression

Of the cytokinesis-related genes, 26 kinesins or kinesin-like genes and genes related to microtubule and myosin were downregulated in hub1-1, among which are the ... syntaxin (KNOLLE)

Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth

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Gene expression

A total of 13 homeotic genes had an altered expression in hub1-1, among which ... a BRAHMA-like protein (At3g06010) of the Polycomb group of proteins in fruitfly (Dro- sophila melanogaster

Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth

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Gene expression

Of the cytokinesis-related genes, 26 kinesins or kinesin-like genes and genes related to microtubule and myosin were downregulated in hub1-1, among which are the ... MAP63-3 (PLEIADE)

Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth

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Gene expression

Of the cytokinesis-related genes, 26 kinesins or kinesin-like genes and genes related to microtubule and myosin were downregulated in hub1-1, among which are the kinesins HINKEL (NACK1)

Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth

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Gene expression

Biological processes, such as response to stress, proton trans- port, cell redox homeostasis, hexose metabolism, cellular respi- ration, and cofactor catabolism, were significantly overexpressed in hub1-1

Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth

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Gene expression

Real time PCR experiments ... in trn2-2 3010 mutant ... did not substantiate a change in WUS transcripts

Chiu WH, Chandler J, Cnops G, Van Lijsebettens M, Werr W - Mutations in the TORNADO2 gene affect cellular decisions in the peripheral zone of the shoot apical meristem of Arabidopsis thaliana

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Gene expression

STM promoter activity ... increased ... in line 3010

Chiu WH, Chandler J, Cnops G, Van Lijsebettens M, Werr W - Mutations in the TORNADO2 gene affect cellular decisions in the peripheral zone of the shoot apical meristem of Arabidopsis thaliana

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Gene expression

trn2-2 ... mutant meristem ... CLV3 ... expression ... show no major differences in size or position relative to wild type

Chiu WH, Chandler J, Cnops G, Van Lijsebettens M, Werr W - Mutations in the TORNADO2 gene affect cellular decisions in the peripheral zone of the shoot apical meristem of Arabidopsis thaliana

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Gene expression

trn2-2 ... mutant meristem ... WUS expression ... show no major differences in size or position relative to wild type

Chiu WH, Chandler J, Cnops G, Van Lijsebettens M, Werr W - Mutations in the TORNADO2 gene affect cellular decisions in the peripheral zone of the shoot apical meristem of Arabidopsis thaliana

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Gene expression

a comparison between trn2-2 3010 seedlings and wild type controls ... shows no significant difference in LFY transcriptional activity in the vegetative SAM

Chiu WH, Chandler J, Cnops G, Van Lijsebettens M, Werr W - Mutations in the TORNADO2 gene affect cellular decisions in the peripheral zone of the shoot apical meristem of Arabidopsis thaliana

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Gene expression

TRN2 transcriptional activity was detected in all types of meristems

Chiu WH, Chandler J, Cnops G, Van Lijsebettens M, Werr W - Mutations in the TORNADO2 gene affect cellular decisions in the peripheral zone of the shoot apical meristem of Arabidopsis thaliana

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Gene expression

STM transcript levels in trn2-2 3010 mutant seedlings ... 4-fold increase

Chiu WH, Chandler J, Cnops G, Van Lijsebettens M, Werr W - Mutations in the TORNADO2 gene affect cellular decisions in the peripheral zone of the shoot apical meristem of Arabidopsis thaliana

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Gene expression

TRN2 transcripts in leaf primorida are preferentially found in vascular strands

Chiu WH, Chandler J, Cnops G, Van Lijsebettens M, Werr W - Mutations in the TORNADO2 gene affect cellular decisions in the peripheral zone of the shoot apical meristem of Arabidopsis thaliana

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Gene expression

TRN2 transcripts in leaf primorida are preferentially found ... at the distal tip of the leaflet

Chiu WH, Chandler J, Cnops G, Van Lijsebettens M, Werr W - Mutations in the TORNADO2 gene affect cellular decisions in the peripheral zone of the shoot apical meristem of Arabidopsis thaliana

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Gene expression

nor quantification of the STM RNA suggests there are significant quantitative changes in STM expression between mutant and wild type inflorescences

Chiu WH, Chandler J, Cnops G, Van Lijsebettens M, Werr W - Mutations in the TORNADO2 gene affect cellular decisions in the peripheral zone of the shoot apical meristem of Arabidopsis thaliana

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Gene expression

TRN2 ... transcripts are ... detectable in lateral organ primordia

Chiu WH, Chandler J, Cnops G, Van Lijsebettens M, Werr W - Mutations in the TORNADO2 gene affect cellular decisions in the peripheral zone of the shoot apical meristem of Arabidopsis thaliana

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Gene expression

skb1 mutants and wild-type plants did not show a significant difference in transcript levels of ... VRN1

Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis

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Gene expression

Loss of SKB1 ... upregulated EMF1

Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis

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Gene expression

SKB1 protein levels were ... slightly decreased with age under long-day conditions

Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis

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Gene expression

Consistent with the protein expression pattern, SKB1 RNA was more abundant in ... leaf primordia

Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis

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Gene expression

Consistent with the protein expression pattern, SKB1 RNA was more abundant in ... young leaves

Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis

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Gene expression

skb1 mutants and wild-type plants did not show a significant difference in transcript levels of ... SPY

Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis

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Gene expression

Consistent with the protein expression pattern, SKB1 ... expressed ... not as much in older leaves

Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis

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Gene expression

Loss of SKB1 downregulated ... FT

Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis

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Gene expression

Consistent with the protein expression pattern, SKB1 RNA was more abundant in shoot apex

Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis

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Gene expression

Loss of SKB1 downregulated ... SOC1

Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis

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Gene expression

Loss of SKB1 ... upregulated ... EMF2

Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis

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Gene expression

SKB1 protein levels were weakly increased with plant age under short-day condition

Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis

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Gene expression

Loss of SKB1 downregulated LFY

Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis

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Gene expression

skb1 mutants and wild-type plants did not show a significant difference in transcript levels of ... CO

Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis

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Gene expression

Western blotting results showed that SKB1 was highly expressed in flowers, roots and siliques, but less so in stems and mature leaves

Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis

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Gene expression

The expression of the flowering repressor gene FLC, however, was significantly upregulated in skb1-1 mutant plants

Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis

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Gene expression

skb1 mutants and wild-type plants did not show a significant difference in transcript levels of GI

Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis

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Gene expression

Loss of SKB1 ... upregulated ... TFL1

Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis

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Gene expression

The expression of LD, in the autonomous pathway upstream of FLC (He and Amasino, 2005), did not differ between skb1-1 and wild-type plants

Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis

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Gene expression

skb1 mutants and wild-type plants did not show a significant difference in transcript levels of ... VRN2

Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis

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Gene expression

P35S:GFP:PARL1 ... nuclear fluores- cence was most strongly localized to a subnuclear re- gion, presumably the nucleolus

Petricka JJ, Nelson TM - Arabidopsis nucleolin affects plant development and patterning

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Gene expression

When the P35S:GFP:PARL1 con- struct was viewed in the same cells under UV illumi- nation, nuclear fluorescence was observed in cotyledon (Fig. 5K) and leaf (Fig. 5N) cells

Petricka JJ, Nelson TM - Arabidopsis nucleolin affects plant development and patterning

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Gene expression

In young leaf primor- dia PPARL1:GUS expression was initially strong and ubiquitous (Fig. 5, D and F), whereas later expression was restricted to the leaf tip and developing vascular cells

Petricka JJ, Nelson TM - Arabidopsis nucleolin affects plant development and patterning

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Gene expression

In parl1 mutant leaves, DR5:GUS expression was initially correctly localized in the tip at comparable stages, suggesting the incipient midvein pattern was normal

Petricka JJ, Nelson TM - Arabidopsis nucleolin affects plant development and patterning

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Gene expression

PARL1 was expressed ... ubiqui- tously throughout the globular stage embryo

Petricka JJ, Nelson TM - Arabidopsis nucleolin affects plant development and patterning

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Gene expression

As auxin response was disrupted in parl1 leaves, we wanted to investigate the role of auxin transport in parl1/DR5:GUS expressing leaves. In wild-type leaves, the inhibition of PAT by naphthylphthalamic acid (NPA) and similar inhibitors leads to restriction of DR5:GUS expression to the leaf margins, possibly re- flecting the accumulation of auxin in these regions (Fig. 6, N and P; Mattsson et al., 2003). Subsequent venation is restricted to the leaf margins. In agreement with this previous report, 1 mM NPA treatment of wild- type plants restricted DR5:GUS expression to the leaf margin (Fig. 6, N and P; Mattsson et al., 2003). In con- trast, this marginal expression was absent in 1 mM NPA-treated leaves of the parl1 mutant (compare Fig. 6, N and P with Fig. 6, O and Q). Thus the expression pattern of DR5:GUS was similar in both NPA-treated and -untreated parl1 leaves

Petricka JJ, Nelson TM - Arabidopsis nucleolin affects plant development and patterning

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Gene expression

Expression of the PPARL1:GUS construct was enriched in vascular cells throughout the seedling and was vascular spe- cific in cotyledons

Petricka JJ, Nelson TM - Arabidopsis nucleolin affects plant development and patterning

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Gene expression

Athb8:GUS is a re- porter line that marks cells in the provascular stage of development before lignification is observed and is one of the earliest characterized reporters for vein de- velopment (Baima et al., 1995; Kang and Dengler, 2002; Scarpella et al., 2004, 2006). In wild-type plants, ex- pression of this reporter was initially visible in both wild-type and the parl1/Athb8:GUS line leaf primor- dia as a single file of expression that extended from the petiole to the tip of leaf blade, marking cells that later differentiate into the midvein (Fig. 6A, inset). Next, as previously reported, two pairs of expression traces connecting to the central line in high-arching loops were observed in the Athb8:GUS line that mark cells destined to become secondary vein loops (Fig. 6A). While parl1 mutants resembled the wild type for early midvein expression, in subsequent stages multiple traces of Athb8:GUS expression extended from the petiole into the leaf primordia, and often ended freely (Fig. 6B). This expression pattern change continued with subsequent vein initiation in parl1 (Fig. 6D). In wild-type plants, the expression of Athb8:GUS marked the closed, interconnected vein pattern observed in normal leaves at later stages of development and expansion (Fig. 6C). However, parl1 at this same stage displayed an Athb8:GUS expression pattern that was unconnected and consisted of freely ending parallel veins

Petricka JJ, Nelson TM - Arabidopsis nucleolin affects plant development and patterning

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Gene expression

This apparently normal tip expression of DR5 persisted into the next stage, when subsequent veins were initiated. How- ever, parl1 mutants failed to show DR5:GUS expres- sion in apical loops, as is observed in wild type (Fig. 6J) and subsequently failed to differentiate veins in apical loops. At the next stage and subsequent stages, the persistent tip expression typical of DR5:GUS in wild- type leaves was drastically mislocalized in parl1 leaves of comparable stages (compare Fig. 6, K–M with Fig. 6, G and H). In the mutant, expression ceased at the tip, and ectopic DR5:GUS expression appeared in one or two foci offset from the tip. This mislocalization was observed at 9 d after germination (DAG) in 66% (n 5 502) and at 10 DAG in 86% (n 5 349) of parl1/DR5:GUS leaves and never (7 DAG, n 5 178; 8 DAG, n 5 274) in the wild type

Petricka JJ, Nelson TM - Arabidopsis nucleolin affects plant development and patterning

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Gene expression

Further analysis of the PPARL1: GUS construct in other tissues revealed expression of the PARL1 gene in regions associated with cell divi- sion (root and leaf tips) as well as in vascular cells of roots and flowers

Petricka JJ, Nelson TM - Arabidopsis nucleolin affects plant development and patterning

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Gene expression

PARL1 ... ubiquitous GUS expression was also observed later in ... globular ... stages of embry- onic development

Petricka JJ, Nelson TM - Arabidopsis nucleolin affects plant development and patterning

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Gene expression

PARL1 ... ubiquitous GUS expression was also observed later in ... the ... heart stages of embry- onic development

Petricka JJ, Nelson TM - Arabidopsis nucleolin affects plant development and patterning

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Gene expression

In young leaf primor- dia PPARL1:GUS expression was initially strong and ubiquitous

Petricka JJ, Nelson TM - Arabidopsis nucleolin affects plant development and patterning

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Gene expression

In young leaf primor- dia PPARL1:GUS expression was initially strong and ubiquitous (Fig. 5, D and F), whereas later expression was restricted to the leaf tip and developing vascular cells

Petricka JJ, Nelson TM - Arabidopsis nucleolin affects plant development and patterning

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Gene expression

in the vfb1-1 vfb2-1 vfb3-1 vfb4-1 (RNAi) mutants ... The group of repressed auxin signaling genes includes the AUX/IAA repressors ... IAA29

Schwager KM, Calderon-Villalobos LI, Dohmann EM, Willige BC, Knierer S, Nill C, Schwechheimer C - Characterization of the VIER F-BOX PROTEINE genes from Arabidopsis reveals their importance for plant growth and development

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Gene expression

the promoter:GUS constructs for VFB1 ... showed strong GUS staining in many plant tissues, including the vascular system, leaves, flowers, and roots

Schwager KM, Calderon-Villalobos LI, Dohmann EM, Willige BC, Knierer S, Nill C, Schwechheimer C - Characterization of the VIER F-BOX PROTEINE genes from Arabidopsis reveals their importance for plant growth and development

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Gene expression

in the vfb1-1 vfb2-1 vfb3-1 vfb4-1 (RNAi) mutants ... The group of repressed auxin signaling genes includes the AUX/IAA repressors ... IAA3 (SHORT HYPOCOTYL2 [SHY2])

Schwager KM, Calderon-Villalobos LI, Dohmann EM, Willige BC, Knierer S, Nill C, Schwechheimer C - Characterization of the VIER F-BOX PROTEINE genes from Arabidopsis reveals their importance for plant growth and development

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Gene expression

in the vfb1-1 vfb2-1 vfb3-1 vfb4-1 (RNAi) mutants ... The group of repressed auxin signaling genes includes the AUX/IAA repressors ... IAA6

Schwager KM, Calderon-Villalobos LI, Dohmann EM, Willige BC, Knierer S, Nill C, Schwechheimer C - Characterization of the VIER F-BOX PROTEINE genes from Arabidopsis reveals their importance for plant growth and development

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Gene expression

in the vfb1-1 vfb2-1 vfb3-1 vfb4-1 (RNAi) mutants ... The group of repressed auxin signaling genes includes the AUX/IAA repressors ... IAA19 (MASSUGU2 [MSG2])

Schwager KM, Calderon-Villalobos LI, Dohmann EM, Willige BC, Knierer S, Nill C, Schwechheimer C - Characterization of the VIER F-BOX PROTEINE genes from Arabidopsis reveals their importance for plant growth and development

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Gene expression

35S:VFB2:GFP ... and 35S:YFP:VFB2 localize to the cytoplasm when expressed in a transient manner in Arabidopsis protoplasts ... Similarly, a VFB2 promoter (2-kb fragment)–driven VFB2:GFP fusion protein also localized predominantly to the cytoplasm (data not shown

Schwager KM, Calderon-Villalobos LI, Dohmann EM, Willige BC, Knierer S, Nill C, Schwechheimer C - Characterization of the VIER F-BOX PROTEINE genes from Arabidopsis reveals their importance for plant growth and development

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Gene expression

VFB2 ... also seem to be expressed in the cotyledons of bent cotyledon stage embryos but not at earlier stages of embryo development

Schwager KM, Calderon-Villalobos LI, Dohmann EM, Willige BC, Knierer S, Nill C, Schwechheimer C - Characterization of the VIER F-BOX PROTEINE genes from Arabidopsis reveals their importance for plant growth and development

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Gene expression

the promoter:GUS constructs for ... VFB2 ... showed strong GUS staining in many plant tissues, including the vascular system, leaves, flowers, and roots

Schwager KM, Calderon-Villalobos LI, Dohmann EM, Willige BC, Knierer S, Nill C, Schwechheimer C - Characterization of the VIER F-BOX PROTEINE genes from Arabidopsis reveals their importance for plant growth and development

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Gene expression

35S:VFB4:GFP ... localize to the cytoplasm when expressed in a transient manner in Arabidopsis protoplasts

Schwager KM, Calderon-Villalobos LI, Dohmann EM, Willige BC, Knierer S, Nill C, Schwechheimer C - Characterization of the VIER F-BOX PROTEINE genes from Arabidopsis reveals their importance for plant growth and development

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Gene expression

VFB4 also seem to be expressed in the cotyledons of bent cotyledon stage embryos but not at earlier stages of embryo development

Schwager KM, Calderon-Villalobos LI, Dohmann EM, Willige BC, Knierer S, Nill C, Schwechheimer C - Characterization of the VIER F-BOX PROTEINE genes from Arabidopsis reveals their importance for plant growth and development

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Gene expression

in the vfb1-1 vfb2-1 vfb3-1 vfb4-1 (RNAi) mutants ... In agreement with previous reports, we detected DR5:GUS staining in the leaf margins, at sites of lateral root initiation, and in the root tips of wild-type seedlings. Interestingly, the staining at these sites was reduced in the vfb mutant background, suggesting—in agreement with our microaray data—that auxin-responsive gene expression is impaired in the vfb mutant background

Schwager KM, Calderon-Villalobos LI, Dohmann EM, Willige BC, Knierer S, Nill C, Schwechheimer C - Characterization of the VIER F-BOX PROTEINE genes from Arabidopsis reveals their importance for plant growth and development

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Gene expression

in the vfb1-1 vfb2-1 vfb3-1 vfb4-1 (RNAi) mutants ... The group of repressed auxin signaling genes includes the AUX/IAA repressors ... IAA5

Schwager KM, Calderon-Villalobos LI, Dohmann EM, Willige BC, Knierer S, Nill C, Schwechheimer C - Characterization of the VIER F-BOX PROTEINE genes from Arabidopsis reveals their importance for plant growth and development

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Gene expression

in the vfb1-1 vfb2-1 vfb3-1 vfb4-1 (RNAi) mutants ... The group of repressed auxin signaling genes includes the AUX/IAA repressors ... IAA2

Schwager KM, Calderon-Villalobos LI, Dohmann EM, Willige BC, Knierer S, Nill C, Schwechheimer C - Characterization of the VIER F-BOX PROTEINE genes from Arabidopsis reveals their importance for plant growth and development

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Gene expression

in the vfb1-1 vfb2-1 vfb3-1 vfb4-1 (RNAi) mutants ... The group of repressed auxin signaling genes includes the AUX/IAA repressors IAA1 (AXR5)

Schwager KM, Calderon-Villalobos LI, Dohmann EM, Willige BC, Knierer S, Nill C, Schwechheimer C - Characterization of the VIER F-BOX PROTEINE genes from Arabidopsis reveals their importance for plant growth and development

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Gene expression

the promoter:GUS constructs for ... VFB4 showed strong GUS staining in many plant tissues, including the vascular system, leaves, flowers, and roots

Schwager KM, Calderon-Villalobos LI, Dohmann EM, Willige BC, Knierer S, Nill C, Schwechheimer C - Characterization of the VIER F-BOX PROTEINE genes from Arabidopsis reveals their importance for plant growth and development

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Gene expression

We found that, in 4-day-old seedlings, AtOFP1prom:GUS was expressed mainly in the roots and lower part of hypocotyls, but not in root hairs, lateral root primordium or shoot (Figure 5a). AtOFP1prom:GUS was still expressed in the roots of 10-day-old seedlings including root hair (Figure 5b), but unlike the 4-day-old seedlings, it was also expressed in shoots, vasculatures and trichomes

Wang S, Chang Y, Guo J, Chen JG - Arabidopsis Ovate Family Protein 1 is a transcriptional repressor that suppresses cell elongation

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Gene expression

By using these transgenic plants, we found that AtOFP1 is predominantly localized in the nucleus ... In a transient expression system using protoplasts derived from Arabidopsis rosette leaves, we also observed a nuclear-localized AtOFP1–GFP fusion protein when the 35S promoter was used to drive AtOFP1–GFP expression (Figure 5h), which is largely consistent with the results reported by Hackbusch et al. (2005), except that we did not observe a significant association of AtOFP1–GFP with the cytoskeleton

Wang S, Chang Y, Guo J, Chen JG - Arabidopsis Ovate Family Protein 1 is a transcriptional repressor that suppresses cell elongation

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Gene expression

We found that, in 4-day-old seedlings, AtOFP1prom:GUS was expressed mainly in the roots and lower part of hypocotyls, but not in root hairs, lateral root primordium or shoot (Figure 5a). AtOFP1prom:GUS was still expressed in the roots of 10-day-old seedlings including root hair (Figure 5b), but unlike the 4-day-old seedlings, it was also expressed in shoots, vasculatures and trichomes

Wang S, Chang Y, Guo J, Chen JG - Arabidopsis Ovate Family Protein 1 is a transcriptional repressor that suppresses cell elongation

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Gene expression

we found AtOFP1 transcript in the roots of young seedlings and both roots and shoots of relatively old seedlings

Wang S, Chang Y, Guo J, Chen JG - Arabidopsis Ovate Family Protein 1 is a transcriptional repressor that suppresses cell elongation

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Gene expression

We found that, in 4-day-old seedlings, AtOFP1prom:GUS was expressed mainly in the roots and lower part of hypocotyls, but not in root hairs, lateral root primordium or shoot (Figure 5a). AtOFP1prom:GUS was still expressed in the roots of 10-day-old seedlings including root hair (Figure 5b), but unlike the 4-day-old seedlings, it was also expressed in shoots, vasculatures and trichomes

Wang S, Chang Y, Guo J, Chen JG - Arabidopsis Ovate Family Protein 1 is a transcriptional repressor that suppresses cell elongation

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Gene expression

Transgenic plants were generated carrying the GUS reporter gene driven by a 2.2 kb BEN1 promoter to examine tissue-specific expression patterns of BEN1 ... In mature rosette leaves, GUS expression was seen mainly in ... hydathodes

Yuan T, Fujioka S, Takatsuto S, Matsumoto S, Gou X, He K, Russell SD, Li J - BEN1, a gene encoding a dihydroflavonol 4-reductase (DFR)-like protein, regulates the levels of brassinosteroids in Arabidopsis thaliana

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Gene expression

the expression of BEN1 is indeed significantly downregulated in darkness

Yuan T, Fujioka S, Takatsuto S, Matsumoto S, Gou X, He K, Russell SD, Li J - BEN1, a gene encoding a dihydroflavonol 4-reductase (DFR)-like protein, regulates the levels of brassinosteroids in Arabidopsis thaliana

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Gene expression

BAS1 was significantly downregulated ... in ben1-1D mutant plants, compared to wild-type plants

Yuan T, Fujioka S, Takatsuto S, Matsumoto S, Gou X, He K, Russell SD, Li J - BEN1, a gene encoding a dihydroflavonol 4-reductase (DFR)-like protein, regulates the levels of brassinosteroids in Arabidopsis thaliana

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Gene expression

Transgenic plants were generated carrying the GUS reporter gene driven by a 2.2 kb BEN1 promoter to examine tissue-specific expression patterns of BEN1 ... In mature rosette leaves, GUS expression was seen mainly in vascular tissues

Yuan T, Fujioka S, Takatsuto S, Matsumoto S, Gou X, He K, Russell SD, Li J - BEN1, a gene encoding a dihydroflavonol 4-reductase (DFR)-like protein, regulates the levels of brassinosteroids in Arabidopsis thaliana

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Gene expression

RT-PCR results also showed that BEN1 transcripts can be detected in all major organs tested, with the strongest signals detected in roots and siliques

Yuan T, Fujioka S, Takatsuto S, Matsumoto S, Gou X, He K, Russell SD, Li J - BEN1, a gene encoding a dihydroflavonol 4-reductase (DFR)-like protein, regulates the levels of brassinosteroids in Arabidopsis thaliana

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Gene expression

CPD is downregulated when BR levels are increased

Yuan T, Fujioka S, Takatsuto S, Matsumoto S, Gou X, He K, Russell SD, Li J - BEN1, a gene encoding a dihydroflavonol 4-reductase (DFR)-like protein, regulates the levels of brassinosteroids in Arabidopsis thaliana

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Gene expression

The subcellular localization revealed using bri1-5 35S-BEN1–GFP should thus represent the real in vivo site of the BEN1 protein. Confocal analyses indicated that BEN1 is a cytoplasmic protein

Yuan T, Fujioka S, Takatsuto S, Matsumoto S, Gou X, He K, Russell SD, Li J - BEN1, a gene encoding a dihydroflavonol 4-reductase (DFR)-like protein, regulates the levels of brassinosteroids in Arabidopsis thaliana

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Gene expression

RT-PCR analyses also indicated that the expression of BEN1 in wild-type plants cannot be significantly regulated by exogenously applied BL

Yuan T, Fujioka S, Takatsuto S, Matsumoto S, Gou X, He K, Russell SD, Li J - BEN1, a gene encoding a dihydroflavonol 4-reductase (DFR)-like protein, regulates the levels of brassinosteroids in Arabidopsis thaliana

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Gene expression

BAS1 is upregulated in response to exogenously elevated BR levels

Yuan T, Fujioka S, Takatsuto S, Matsumoto S, Gou X, He K, Russell SD, Li J - BEN1, a gene encoding a dihydroflavonol 4-reductase (DFR)-like protein, regulates the levels of brassinosteroids in Arabidopsis thaliana

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Gene expression

CPD was significantly upregulated in ben1-1D mutant plants, compared to wild-type plants

Yuan T, Fujioka S, Takatsuto S, Matsumoto S, Gou X, He K, Russell SD, Li J - BEN1, a gene encoding a dihydroflavonol 4-reductase (DFR)-like protein, regulates the levels of brassinosteroids in Arabidopsis thaliana

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Gene expression

level of transcripts of the ... KAN2 ... increased in the ... as1-1

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

the level of ARF4 did not clearly increase in as2-1

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

in as1-1 ... No significant differences in levels of transcripts were detected for ... YAB3

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

in as2-1 ... No significant differences in levels of transcripts were detected for ... YAB2

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

in as2-1 ... No significant differences in levels of transcripts were detected for ... YAB3

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

AS1 transcripts ... As primordia grew (P4–P6), we detected strong signals in the central region between the adaxial and abaxial domains (Figure 1l,m). The

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

level of transcripts of the ETT/ARF3 ... increased in the as2-1

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

Levels of ARF2 transcripts were not affected by ... as2

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

level of transcripts of the ... KAN2 ... decreased in the as2-1/pAS1::AS2#4 plants

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

The level of FIL transcripts ... did not clearly increase in as1-1

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

level of transcripts of the ... class 1 KNOX genes, increased in the as2-1

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

in as1-1 ... No significant differences in levels of transcripts were detected for ... KAN3

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

level of transcripts of the ... class 1 KNOX genes ... decreased in the as2-1/pAS1::AS2#4 plants

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

Levels of ARF2 transcripts were not affected by ... as1

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

in as2-1 ... No significant differences in levels of transcripts were detected for ... PHV

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

level of transcripts of the ... KAN2 ... increased in the as2-1

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

We obtained five independent transgenic lines that harbored pAS2::GUS and all yielded essentially the same pattern of expression of GUS activity ... When leaves had developed (21-day-old plants), we detected GUS activity over the entire adaxial surface of leaves

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

level of transcripts of the ETT/ARF3 ... increased in the ... as1-1

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

The level of FIL transcripts ... decreased in the as2-1/pAS1::AS2#4 plants

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

We obtained five independent transgenic lines that harbored pAS2::GUS and all yielded essentially the same pattern of expression of GUS activity ... we detected GUS activity on the adaxial sides of cotyledons

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

We obtained eight independent pAS1::GUS transgenic lines and detected GUS activity mainly in inner tissues, which included the vascu- lature of leaves, hypocotyls and roots

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

Levels of transcripts of the BP/KNAT1 ... decreased in as2-1/pAS1::AS2#4 plants

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

The level of FIL transcripts increased in as2-1

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

We obtained five independent transgenic lines that harbored pAS2::GUS and all yielded essentially the same pattern of expression of GUS activity ... The activity in cotyledons and leaves disappeared as the leaves grew further

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

level of transcripts of the ... YAB5 ... increased in the ... as1-1

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

In P0– P3, we detected weak signals due to AS1 in entire primordia

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

in as2-1 ... No significant differences in levels of transcripts were detected for KAN1

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

level of transcripts of the ... YAB5 ... increased in the as2-1

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

level of transcripts of the ... YAB5 ... decreased in the as2-1/pAS1::AS2#4 plants

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

Levels of transcripts of the ... KNAT6 ... decreased in as2-1/pAS1::AS2#4 plants

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

AS1 transcripts ... In more developed primordia (after P8), we detected signals in both the vasculature and lateral regions

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

in as1-1 ... No significant differences in levels of transcripts were detected for KAN1

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

AS1 transcripts were clearly evident in the inner tissues of leaves (Figure 1l,m), as reported by Byrne et al. (2000

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

the level of ARF4 ... decreased in the as2-1/pAS1::AS2#4 plants

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

The level of ARF4 transcripts increased in as1-1

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

Levels of transcripts of the ... KNAT6 ... increased in the ... as1-1

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

level of transcripts of the ... class 1 KNOX genes, increased in the ... as1-1

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

in as1-1 ... No significant differences in levels of transcripts were detected for ... PHV

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

Levels of transcripts of the ... KNAT6 ... increased in the as2-1

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

in as1-1 ... No significant differences in levels of transcripts were detected for ... KAN4

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

in as2-1 ... No significant differences in levels of transcripts were detected for ... PHB

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

in as2-1 ... No significant differences in levels of transcripts were detected for ... KAN4

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

The level of the KNAT2 gene transcript was not clearly altered in shoot apices of as2-1

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

Levels of transcripts of the BP/KNAT1 ... increased in the ... as1-1

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

We performed in situ hybridization in order to detect AS2 transcripts in plantlets ... When primordia had developed, we detected stronger signals in the adaxial region, including the epidermis of leaf primordia. However, weak signals were also evident in the inner region (P3–P9; Figure 1a–f

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

We obtained eight independent pAS1::GUS transgenic lines ... When leaves had developed (24-day-old plants), GUS signals in the vasculature of cotyledons and leaves became weaker, as previously shown by RT-PCR (Figure 1p) (Chua et al., 2005)

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

We performed in situ hybridization in order to detect AS2 transcripts in plantlets. In transverse and longitudinal sections of 12-day-old plants, we detected relatively weak signals due to AS2 transcripts in the region of the predicted leaf primordium (P0) and in early detectable primordia (P1 and P2) (Figure 1a–f,r

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

in as1-1 ... No significant differences in levels of transcripts were detected for ... YAB2

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

We obtained five independent transgenic lines that harbored pAS2::GUS and all yielded essentially the same pattern of expression of GUS activity ... we detected GUS activity on the adaxial sides of ... leaves

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

Levels of transcripts of the BP/KNAT1 ... increased in the as2-1

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

in as2-1 ... No significant differences in levels of transcripts were detected for ... KAN3

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

in as1-1 ... No significant differences in levels of transcripts were detected for ... PHB

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

in as2-1 ... No significant differences in levels of transcripts were detected for ... REV

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

level of transcripts of the ETT/ARF3 ... decreased in the as2-1/pAS1::AS2#4 plants

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

in as1-1 ... No significant differences in levels of transcripts were detected for ... REV

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

AS1 transcripts ... In more developed primordia (after P8), we detected signals in ... the vasculature

Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves

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Gene expression

pANT::GUS ... GUS activity was seen throughout very young leaves at Day 0

Kang J, Mizukami Y, Wang H, Fowke L, Dengler NG - Modification of cell proliferation patterns alters leaf vein architecture in Arabidopsis thaliana

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Gene expression

pANT::GUS ... GUS activity ... declined basipetally in the leaf blades at Day 4

Kang J, Mizukami Y, Wang H, Fowke L, Dengler NG - Modification of cell proliferation patterns alters leaf vein architecture in Arabidopsis thaliana

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Gene expression

Cross sections of the leaf revealed that pANT::GUS expression was seen in procambial cells prior to vascular differentiation (Fig. 2b), remained in procambial cells during vein differentiation (Fig. 2b asterisks), and declined as procambial cells differentiated into xylem and phloem

Kang J, Mizukami Y, Wang H, Fowke L, Dengler NG - Modification of cell proliferation patterns alters leaf vein architecture in Arabidopsis thaliana

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Gene expression

pANT::GUS ... GUS activity ... remained high in developing veins through to Day 8

Kang J, Mizukami Y, Wang H, Fowke L, Dengler NG - Modification of cell proliferation patterns alters leaf vein architecture in Arabidopsis thaliana

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Gene expression

the number of cells accumulating CYCB1;1::GUS in the apical half of leaves was similar to that in wild-type leaves at Day 0, there appeared to be fewer GUS expressing cells in ant-1 leaves by Day 4 compared to wild-type leaves at the same stage

Kang J, Mizukami Y, Wang H, Fowke L, Dengler NG - Modification of cell proliferation patterns alters leaf vein architecture in Arabidopsis thaliana

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Gene expression

pANT::GUS expression in procambial cells disappeared after leaf maturation (data not shown)

Kang J, Mizukami Y, Wang H, Fowke L, Dengler NG - Modification of cell proliferation patterns alters leaf vein architecture in Arabidopsis thaliana

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Gene expression

In 35S::ICK1 leaves, CYCB1;1::GUS accumulation was sharply reduced in comparison to wild-type at all stages, reflecting a repression of cell proliferation throughout leaf development

Kang J, Mizukami Y, Wang H, Fowke L, Dengler NG - Modification of cell proliferation patterns alters leaf vein architecture in Arabidopsis thaliana

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Gene expression

mRNA level of FLOWERING LOCUS K (FLK), a component of autonomous pathway, was increased by 2-fold ... in atprmt5 mutants as compared to wild-type

Pei Y, Niu L, Lu F, Liu C, Zhai J, Kong X, Cao X - Mutations in the Type II protein arginine methyltransferase AtPRMT5 result in pleiotropic developmental defects in Arabidopsis

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Gene expression

Expression of FLC was dramatically increased due to mutations in AtPRMT5

Pei Y, Niu L, Lu F, Liu C, Zhai J, Kong X, Cao X - Mutations in the Type II protein arginine methyltransferase AtPRMT5 result in pleiotropic developmental defects in Arabidopsis

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Gene expression

due to mutations in AtPRMT5 ... expression of SUPPRESSOR OF OVEREXPRESSION OF CO1 (SOC1)/AGAMOUS-LIKE20, a floral integrator downstream of FLC, was greatly reduced

Pei Y, Niu L, Lu F, Liu C, Zhai J, Kong X, Cao X - Mutations in the Type II protein arginine methyltransferase AtPRMT5 result in pleiotropic developmental defects in Arabidopsis

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Gene expression

mRNA level of ... ARP6 (ACTIN-RELATED PROTEIN6), an activator of FLC, was decreased by 2-fold in atprmt5 mutants as compared to wild-type Col

Pei Y, Niu L, Lu F, Liu C, Zhai J, Kong X, Cao X - Mutations in the Type II protein arginine methyltransferase AtPRMT5 result in pleiotropic developmental defects in Arabidopsis

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Gene expression

no FIL expression was detected in ... as1 ... differentiated leaves

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

we could not detect a significant change in the expression levels of FIL ... in ... bop1-4 bop2-11 plants

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

FIL transcripts were likewise detected on the adaxial side of bop1 bop2 as2 ... differentiated leaves but with the expression in the more central region of the leaf

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

The expression levels of ... KNAT2 ... were significantly increased in bop1-4 bop2-11 seedlings

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

The domain of PHV expression is unaltered in ... as1 seedlings

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

LBD36/ASL1 transcript levels were elevated in 35S:BOP1 ... plants

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

in ... bop1 bop2 as1 ... plants, FIL expression was detected on the abaxial side of initiating and young leaf primordia

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

LBD36/ASL1 transcript levels were elevated in ... 35S:BOP2 plants

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

in bop1 bop2 ... plants, FIL expression was detected on the abaxial side of initiating and young leaf primordia

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

we could not detect a significant change in the expression levels of FIL ... in ... bop2-11 ... plants

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

AS1 transcript levels were slightly elevated in ... bop1-4 bop2-11 plants compared with the wild type

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

PHV expression is not detected in the differentiated leaves of ... as1

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

we found that PHB transcription was slightly upregulated ... in the developing young leaves of 22-d-old 35S:BOP1 leaves compared with wild-type leaves

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

In as2 ... FIL transcripts are detected in the correct abaxial domain ... of initiating and young leaf primordia

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

The expression levels of BP ... were significantly increased in bop1-4 bop2-11 seedlings

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

in ... bop1 bop2 kan1 kan2 plants, FIL expression was detected on the abaxial side of initiating and young leaf primordia

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

KAN1 transcription was slightly reduced in the developing young leaves of 22-d-old 35S:BOP1 leaves compared with wild-type leaves

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

more mature ... bop1 bop2 as1 ... leaves exhibited ectopic FIL transcription in the adaxial domain

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

AS1 expression levels were unchanged in ... 35S:BOP2 plants

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

we could not detect a significant change in the expression levels of ... KAN1 ... in bop1-4

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

AS2 transcript levels were also reduced in bop1-1 ... plants

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

Expression levels of ... AS2 ... were highly upregulated in 35S:BOP1 ... plants

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

Developing young leaves of ... bop1 bop2 as1 ... exhibited PHV expression in the normal adaxial domain

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

AS1 transcript levels were slightly elevated in bop1-1 ... plants compared with the wild type

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

no FIL expression was detected in as2 ... differentiated leaves

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

LBD36/ASL1 transcript levels ... were reduced in bop1-1 ... plants

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

we could not detect a significant change in the expression levels of FIL ... in bop1-4

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

lob:ET22 GUS ... We also detected ectopic GUS activity broadly across the blade region of 35S:BOP1 leaves ... a pattern that was not observed in lob leaves

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

we could not detect a significant change in the expression levels of ... KAN1 ... in ... bop2-11 ... plants

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

PHV expression ... can be observed on the adaxial side of the differentiated leaves of ... bop1 bop2 as1 ... Furthermore, PHV was expressed more centrally in ... bop1 bop2 as1 ... leaves than in bop1 bop2 leaves

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

The differentiated leaves of bop1 bop2 plants exhibited FIL expression on the adaxial side of the primordial ectopic organ outgrowths (Figure 8P, arrows), while FIL transcripts were not detected in differentiated leaves of Col plants

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

Expression levels of ... LOB were highly upregulated in 35S:BOP1 ... plants

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

In kan1 kan2 seedlings, PHV expression is detected throughout the leaf primordia, but a stronger signal is observed on the adaxial side than on the abaxial side (Figure 9D), suggesting that these organs still maintain some residual asymmetry

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

In bop1-4 bop2-11 plants containing lob:ET22, GUS expression was observed in the shoot apex but more weakly than in lob plants

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

Expression levels of ... LOB were highly upregulated in ... 35S:BOP2 plants

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

in bop1 bop2 leaves, KAN1 expression is detected in the adaxial region in the area in which the ectopic organ outgrowths develop

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

in ... bop1 bop2 as2 ... plants, FIL expression was detected on the abaxial side of initiating and young leaf primordia

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

FIL transcripts were likewise detected on the adaxial side of ... bop1 bop2 kan1 kan2 differentiated leaves but with the expression in the more central region of the leaf

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

PHB is one of the class III HD-ZIP genes that specify adaxial cell fate, and its steady state transcript levels were increased in 8-d-old 35S:BOP1 seedlings compared with wild-type seedlings

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

LOB expression was decreased in ... bop1-4 bop2-11 plants

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

no FIL expression was detected in ... kan1 kan2 differentiated leaves

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

Expression levels of ... AS2 ... were highly upregulated in ... 35S:BOP2 plants

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

Developing young leaves of ... bop1 bop2 as2 ... exhibited PHV expression in the normal adaxial domain

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

PHB ... we could not detect a significant change in the expression levels ... in ... bop1-4 bop2-11 plants

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

The domain of PHV expression is unaltered in as2 ... seedlings

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

in 8-d-old 35S:BOP1 seedlings ... we could not detect a significant change in the expression levels of FIL

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

AS2 transcript levels were also reduced in ... bop1-4 bop2-11 plants

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

more mature ... bop1 bop2 kan1 kan2 leaves exhibited ectopic FIL transcription in the adaxial domain

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

BP mRNA levels were reduced in the shoots of 25-d-old 35S:BOP1 ... plants

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

ectopic expression of BOP1 resulted in ... expanded lob:ET22 GUS expression in the shoot apex

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

more mature ... bop1 bop2 as2 ... leaves exhibited ectopic FIL transcription in the adaxial domain

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

Developing young leaves of ... bop1 bop2 kan1 kan2 seedlings exhibited PHV expression in the normal adaxial domain (Figures 9I to 9L). However, in bop1 bop2 kan1 kan2 leaves, PHV expression is restricted to a smaller area of the adaxial domain than in kan1 kan2 leaves (Figures 9D and 9L, arrow). This result indicates that, whereas the young leaves of kan1 kan2 plants have adaxialized organ identity, the young leaves of quadruple mutant plants acquire abaxialized organ identity, which is consistent with histological data

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

PHV expression is not detected in the differentiated leaves of ... kan1 kan2 plants

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

In lob plants, the lob:ET22 enhancer trap drove GUS reporter gene expression at the shoot apex

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

ectopic expression of BOP1 resulted in increased ... lob:ET22 GUS expression in the shoot apex

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

PHV expression is not detected in the differentiated leaves of ... as2

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

PHB ... we could not detect a significant change in the expression levels ... in ... bop2-11

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

PHV expression ... can be observed on the adaxial side of the differentiated leaves of ... bop1 bop2 kan1 kan2 plants ... Furthermore, PHV was expressed more centrally in ... bop1 bop2 kan1 kan2 leaves than in bop1 bop2 leaves

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

we could not detect a significant change in the expression levels of ... KAN1 ... in ... bop1-4 bop2-11 plants

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

LOB expression was decreased in bop1-1 ... plants

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

BP mRNA levels were reduced in the shoots of 25-d-old ... 35S:BOP2 plants

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

FIL ... transcription was slightly reduced in the developing young leaves of 22-d-old 35S:BOP1 leaves compared with wild-type leaves

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

PHB ... we could not detect a significant change in the expression levels ... in bop1-4 ... plants

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

In bop1-1 plants containing lob:ET22, we could not detect GUS gene activity in the shoot apex

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

PHV expression ... can be observed on the adaxial side of the differentiated leaves of ... bop1 bop2 as2 ... Furthermore, PHV was expressed more centrally in bop1 bop2 as2 ... leaves than in bop1 bop2 leaves

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

In ... kan1 kan2 ... FIL transcripts are detected in the correct abaxial domain ... of initiating and young leaf primordia

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

FIL transcripts were likewise detected on the adaxial side of ... bop1 bop2 as1 ... differentiated leaves but with the expression in the more central region of the leaf

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

In ... as1 ... FIL transcripts are detected in the correct abaxial domain ... of initiating and young leaf primordia

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

AS1 expression levels were unchanged in ... 35S:BOP1 ... plants

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

in 8-d-old 35S:BOP1 seedlings ... we could not detect a significant change in the expression levels of ... KAN1

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

PHV expression ... can be observed on the adaxial side of the differentiated leaves of bop1 bop2

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

Developing young leaves of bop1 bop2 ... exhibited PHV expression in the normal adaxial domain

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

In lob plants, the lob:ET22 enhancer trap drove GUS reporter gene expression at the shoot apex, in the hypocotyl, at the base of all lateral organs, and in the roots

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

The expression levels of ... KNAT6 ... were significantly increased in bop1-4 bop2-11 seedlings

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

more mature bop1 bop2 ... leaves exhibited ectopic FIL transcription in the adaxial domain

Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes

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Gene expression

The abundance of AXL mRNA was extremely low compared with AXR1 mRNA (data not shown). Although AXL RNA was detected in all the tissue types examined, root tissues showed the highest RNA abundance. However, at least 50 PCR cycles were required to obtain a band detectable by standard agarose gel electrophoresis. The relatively low level of expression of AXL in all tissues is also confirmed by microarray analysis

Dharmasiri N, Dharmasiri S, Weijers D, Karunarathna N, Jurgens G, Estelle M - AXL and AXR1 have redundant functions in RUB conjugation and growth and development in Arabidopsis

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Gene expression

the level of the EBP1 mRNA was found to be around twofold ... lower in the ... AtTOR-silenced lines

Deprost D, Yao L, Sormani R, Moreau M, Leterreux G, Nicolaï M, Bedu M, Robaglia C, Meyer C - The Arabidopsis TOR kinase links plant growth, yield, stress resistance and mRNA translation

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Gene expression

A higher expression of the AtEBP1 gene was indeed detected in the AtTOR-overexpressing line S7817 when compared with Col0 after hybridization of CATMA arrays (urgv.evry.inra.fr/cgi-bin/projects/CATdb; D. Deprost & C. Meyer, unpublished data). These preliminary results were confirmed by quantitative real-time reverse transcriptase–PCR and the level of the EBP1 mRNA was found to be around twofold higher ... in the AtTOR-overexpressing ... lines

Deprost D, Yao L, Sormani R, Moreau M, Leterreux G, Nicolaï M, Bedu M, Robaglia C, Meyer C - The Arabidopsis TOR kinase links plant growth, yield, stress resistance and mRNA translation

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Gene expression

CYCD3;1 expression is restricted to the ... very young primordia

Dewitte W, Scofield S, Alcasabas AA, Maughan SC, Menges M, Braun N, Collins C, Nieuwland J, Prinsen E, Sundaresan V, Murray JA - Arabidopsis CYCD3 D-type cyclins link cell proliferation and endocycles and are rate-limiting for cytokinin responses

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Gene expression

CYCD3;2 ... reporters are also active in older leaf primordia

Dewitte W, Scofield S, Alcasabas AA, Maughan SC, Menges M, Braun N, Collins C, Nieuwland J, Prinsen E, Sundaresan V, Murray JA - Arabidopsis CYCD3 D-type cyclins link cell proliferation and endocycles and are rate-limiting for cytokinin responses

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Gene expression

CYCD3;2 expression persisting longest in young leaves

Dewitte W, Scofield S, Alcasabas AA, Maughan SC, Menges M, Braun N, Collins C, Nieuwland J, Prinsen E, Sundaresan V, Murray JA - Arabidopsis CYCD3 D-type cyclins link cell proliferation and endocycles and are rate-limiting for cytokinin responses

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Gene expression

earlier decline in expression of the mitotic B-type cyclin CYCB1;1 in young cycd3;1-3 leaves

Dewitte W, Scofield S, Alcasabas AA, Maughan SC, Menges M, Braun N, Collins C, Nieuwland J, Prinsen E, Sundaresan V, Murray JA - Arabidopsis CYCD3 D-type cyclins link cell proliferation and endocycles and are rate-limiting for cytokinin responses

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Gene expression

CYCD3;3 reporters are also active in older leaf primordia

Dewitte W, Scofield S, Alcasabas AA, Maughan SC, Menges M, Braun N, Collins C, Nieuwland J, Prinsen E, Sundaresan V, Murray JA - Arabidopsis CYCD3 D-type cyclins link cell proliferation and endocycles and are rate-limiting for cytokinin responses

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Gene expression

CYCD3;1 expression is restricted to the ... young hydathodes

Dewitte W, Scofield S, Alcasabas AA, Maughan SC, Menges M, Braun N, Collins C, Nieuwland J, Prinsen E, Sundaresan V, Murray JA - Arabidopsis CYCD3 D-type cyclins link cell proliferation and endocycles and are rate-limiting for cytokinin responses

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Gene expression

CYCD3;1 expression is restricted to the SAM

Dewitte W, Scofield S, Alcasabas AA, Maughan SC, Menges M, Braun N, Collins C, Nieuwland J, Prinsen E, Sundaresan V, Murray JA - Arabidopsis CYCD3 D-type cyclins link cell proliferation and endocycles and are rate-limiting for cytokinin responses

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Gene expression

RNA gel blot analysis revealed that, whereas the expression of PAL1 ... was not significantly changed in siz1-3

Catala R, Ouyang J, Abreu IA, Hu Y, Seo H, Zhang X, Chua NH - The Arabidopsis E3 SUMO ligase SIZ1 regulates plant growth and drought responses

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Gene expression

in 3-week-old plants ... SIZ1 was not expressed ... in the basal region of developing young leaves

Catala R, Ouyang J, Abreu IA, Hu Y, Seo H, Zhang X, Chua NH - The Arabidopsis E3 SUMO ligase SIZ1 regulates plant growth and drought responses

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Gene expression

For example, under control conditions, SQS2 expression in the wild type was approximately threefold higher than in siz1-3 (Figure 5, panel 2, column WT C/siz1-3 C); however, the relative fold induction of this gene by drought was comparable in the mutant and the wild type

Catala R, Ouyang J, Abreu IA, Hu Y, Seo H, Zhang X, Chua NH - The Arabidopsis E3 SUMO ligase SIZ1 regulates plant growth and drought responses

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Gene expression

in siz1-3, the CHS1 transcript accumulation was clearly lower in the mutant compared with the wild type

Catala R, Ouyang J, Abreu IA, Hu Y, Seo H, Zhang X, Chua NH - The Arabidopsis E3 SUMO ligase SIZ1 regulates plant growth and drought responses

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Gene expression

in developing adult leaves, SIZ1 expression was detected in leaf blades and petioles

Catala R, Ouyang J, Abreu IA, Hu Y, Seo H, Zhang X, Chua NH - The Arabidopsis E3 SUMO ligase SIZ1 regulates plant growth and drought responses

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Gene expression

in 3-week-old plants ... SIZ1 was not expressed, at detectable levels, in juvenile leaves

Catala R, Ouyang J, Abreu IA, Hu Y, Seo H, Zhang X, Chua NH - The Arabidopsis E3 SUMO ligase SIZ1 regulates plant growth and drought responses

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Gene expression

RNA gel blot analysis revealed that, whereas the expression of ... CHI was not significantly changed in siz1-3

Catala R, Ouyang J, Abreu IA, Hu Y, Seo H, Zhang X, Chua NH - The Arabidopsis E3 SUMO ligase SIZ1 regulates plant growth and drought responses

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Gene expression

Figure 1 shows the representative expression profile of pSIZ1-2:GUS containing 3535 bp of 5′ upstream sequences ... In 3-d-old germinating seedlings, SIZ1 was expressed in all organs except part of the hypocotyl and the basal region of the cotyledons

Catala R, Ouyang J, Abreu IA, Hu Y, Seo H, Zhang X, Chua NH - The Arabidopsis E3 SUMO ligase SIZ1 regulates plant growth and drought responses

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Gene expression

in developing adult leaves, SIZ1 expression was detected in leaf blades and petioles

Catala R, Ouyang J, Abreu IA, Hu Y, Seo H, Zhang X, Chua NH - The Arabidopsis E3 SUMO ligase SIZ1 regulates plant growth and drought responses

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Gene expression

In the second category, exemplified by HMG1, basal level expression was reduced in siz1-3 compared with the wild type

Catala R, Ouyang J, Abreu IA, Hu Y, Seo H, Zhang X, Chua NH - The Arabidopsis E3 SUMO ligase SIZ1 regulates plant growth and drought responses

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Gene expression

RT-PCR analysis of line E1439 confirmed that expression of DWF4 was absent in these plants

Reinhardt B, Hänggi E, Müller S, Bauch M, Wyrzykowska J, Kerstetter R, Poethig S, Fleming AJ - Restoration of DWF4 expression to the leaf margin of a dwf4 mutant is sufficient to restore leaf shape but not size: the role of the margin in leaf development

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Gene expression

Confocal scanning laser microscopy of the E1439 line revealed that GFP expression was tightly restricted to the epidermal cell layer at the leaf perimeter

Reinhardt B, Hänggi E, Müller S, Bauch M, Wyrzykowska J, Kerstetter R, Poethig S, Fleming AJ - Restoration of DWF4 expression to the leaf margin of a dwf4 mutant is sufficient to restore leaf shape but not size: the role of the margin in leaf development

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Gene expression

our analysis revealed an accumulation of DWF4 mRNAin the epidermis of young leaf primordia

Reinhardt B, Hänggi E, Müller S, Bauch M, Wyrzykowska J, Kerstetter R, Poethig S, Fleming AJ - Restoration of DWF4 expression to the leaf margin of a dwf4 mutant is sufficient to restore leaf shape but not size: the role of the margin in leaf development

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Gene expression

DWF4 transcripts ... became apparent in the vascular tissue

Reinhardt B, Hänggi E, Müller S, Bauch M, Wyrzykowska J, Kerstetter R, Poethig S, Fleming AJ - Restoration of DWF4 expression to the leaf margin of a dwf4 mutant is sufficient to restore leaf shape but not size: the role of the margin in leaf development

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Gene expression

GFP expression observed in the E1439 driver line ... margin pattern is highlighted

Reinhardt B, Hänggi E, Müller S, Bauch M, Wyrzykowska J, Kerstetter R, Poethig S, Fleming AJ - Restoration of DWF4 expression to the leaf margin of a dwf4 mutant is sufficient to restore leaf shape but not size: the role of the margin in leaf development

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Gene expression

DWF4::GUS reporter gene fusion under the control of the UAS promoter ... into the E1439 driver line ... Reporter gene activity was restricted to the leaf margin

Reinhardt B, Hänggi E, Müller S, Bauch M, Wyrzykowska J, Kerstetter R, Poethig S, Fleming AJ - Restoration of DWF4 expression to the leaf margin of a dwf4 mutant is sufficient to restore leaf shape but not size: the role of the margin in leaf development

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Gene expression

DWF4 transcripts became more restricted to the ... abaxial epidermis

Reinhardt B, Hänggi E, Müller S, Bauch M, Wyrzykowska J, Kerstetter R, Poethig S, Fleming AJ - Restoration of DWF4 expression to the leaf margin of a dwf4 mutant is sufficient to restore leaf shape but not size: the role of the margin in leaf development

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Gene expression

DWF4 transcripts became more restricted to the lateral ... epidermis

Reinhardt B, Hänggi E, Müller S, Bauch M, Wyrzykowska J, Kerstetter R, Poethig S, Fleming AJ - Restoration of DWF4 expression to the leaf margin of a dwf4 mutant is sufficient to restore leaf shape but not size: the role of the margin in leaf development

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Gene expression

E1439>>DWF4 ... in situ hybridization analysis detected a low level of DWF4 transcripts only in a small group of cells around the margin of the complemented lines

Reinhardt B, Hänggi E, Müller S, Bauch M, Wyrzykowska J, Kerstetter R, Poethig S, Fleming AJ - Restoration of DWF4 expression to the leaf margin of a dwf4 mutant is sufficient to restore leaf shape but not size: the role of the margin in leaf development

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Gene expression

One of the lines identified by this screen (E1439) showed restriction of GFP expression within the leaf, primarily at the margin

Reinhardt B, Hänggi E, Müller S, Bauch M, Wyrzykowska J, Kerstetter R, Poethig S, Fleming AJ - Restoration of DWF4 expression to the leaf margin of a dwf4 mutant is sufficient to restore leaf shape but not size: the role of the margin in leaf development

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Gene expression

MID:GUS ... Expression was found in the actively proliferating tissues of the shoot and root

Kirik V, Schrader A, Uhrig JF, Hulskamp M - MIDGET unravels functions of the Arabidopsis topoisomerase VI complex in DNA endoreduplication, chromatin condensation, and transcriptional silencing

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Gene expression

MID:GUS ... We also found expression in elongating hypocotyl cells and developing trichomes

Kirik V, Schrader A, Uhrig JF, Hulskamp M - MIDGET unravels functions of the Arabidopsis topoisomerase VI complex in DNA endoreduplication, chromatin condensation, and transcriptional silencing

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Gene expression

TSI expression is activated in the mid ... mutants

Kirik V, Schrader A, Uhrig JF, Hulskamp M - MIDGET unravels functions of the Arabidopsis topoisomerase VI complex in DNA endoreduplication, chromatin condensation, and transcriptional silencing

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Gene expression

We analyzed the expression of the pCYCB1;1:GUS reporter in the mid mutants (Colon-Carmona et al., 1999) and found expression not only in mitotic cells but also in differentiated cells, including cells that have endoreduplicated DNA-like trichomes and hypocotyl cells

Kirik V, Schrader A, Uhrig JF, Hulskamp M - MIDGET unravels functions of the Arabidopsis topoisomerase VI complex in DNA endoreduplication, chromatin condensation, and transcriptional silencing

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Gene expression

TSI expression is activated in the ... fas1 mutants

Kirik V, Schrader A, Uhrig JF, Hulskamp M - MIDGET unravels functions of the Arabidopsis topoisomerase VI complex in DNA endoreduplication, chromatin condensation, and transcriptional silencing

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Gene expression

In 35S:MID-YFP lines, a strong YFP fluorescence signal was found in the nuclei in different cell types, indicating that MID is a nuclear protein

Kirik V, Schrader A, Uhrig JF, Hulskamp M - MIDGET unravels functions of the Arabidopsis topoisomerase VI complex in DNA endoreduplication, chromatin condensation, and transcriptional silencing

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Gene expression

CYC1;4 ... does not appear to differ between the wild type and bin4

Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis

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Gene expression

BIN4 promoter ... GUS signal is also detectable in various tissues that contain proliferating cells, such as root and shoot apical meristems

Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis

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Gene expression

In addition, our real-time RT-PCR analysis revealed that several DSB-inducible genes, including poly (ADP-ribose) polymerase1 (At PARP1 ... were significantly upregulated in bin4

Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis

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Gene expression

The publicly available microarray data, using synchronized Arabidopsis cell cultures (Menges et al., 2005), revealed that BIN4 is ubiquitously expressed at low levels during the mitotic cell cycle (see Supplemental Figure 5 online). This pattern of expression is similar to that of other components of the plant topo VI complex ... i.e ... TOP6B/RHL3/HYP6/BIN3

Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis

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Gene expression

Indeed, we found that the BIN4:GFP (for green fluorescent protein) fusion protein, expressed under the control of a cauliflower mosaic virus 35S promoter in Arabidopsis leaf epidermal cells, is targeted to the nucleus

Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis

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Gene expression

WEE1 ... does not appear to differ between the wild type and bin4

Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis

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Gene expression

CYCA1;1 ... does not appear to differ between the wild type and bin4

Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis

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Gene expression

histone H4 ... does not appear to differ between the wild type and bin4

Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis

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Gene expression

our real-time RT-PCR analyses showed that the transcript level of a G2/M-specific gene, cyclin B1;1 (CYCB1;1), was increased significantly in bin4-1

Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis

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Gene expression

CYCB2;3 ... does not appear to differ between the wild type and bin4

Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis

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Gene expression

CYCB1;2 ... does not appear to differ between the wild type and bin4

Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis

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Gene expression

addition, our real-time RT-PCR analysis revealed that several DSB-inducible genes, including ... breast cancer susceptibility1 (At BRCA1) ... were significantly upregulated in bin4

Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis

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Gene expression

We examined the developmental pattern of the BIN4 promoter activity by fusing the 2-kb genomic region upstream of the BIN4 initiation codon to the β-glucuronidase (GUS) reporter gene. The histochemical analysis revealed that the BIN4 promoter is active in endoreduplicating cells, including those in young, rapidly expanding cotyledons

Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis

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Gene expression

The publicly available microarray data, using synchronized Arabidopsis cell cultures (Menges et al., 2005), revealed that BIN4 is ubiquitously expressed at low levels during the mitotic cell cycle (see Supplemental Figure 5 online). This pattern of expression is similar to that of other components of the plant topo VI complex ... i.e., RHL1/HYP7

Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis

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Gene expression

The publicly available microarray data, using synchronized Arabidopsis cell cultures (Menges et al., 2005), revealed that BIN4 is ubiquitously expressed at low levels during the mitotic cell cycle

Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis

Visit the PubMed Article

Gene expression

The publicly available microarray data, using synchronized Arabidopsis cell cultures (Menges et al., 2005), revealed that BIN4 is ubiquitously expressed at low levels during the mitotic cell cycle (see Supplemental Figure 5 online). This pattern of expression is similar to that of other components of the plant topo VI complex ... i.e ... SPO11-3/RHL2/BIN5

Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis

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Gene expression

CYCD4;1 ... does not appear to differ between the wild type and bin4

Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis

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Gene expression

In addition, our real-time RT-PCR analysis revealed that several DSB-inducible genes, including ... RAD51 ... were significantly upregulated in bin4

Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis

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Gene expression

To further explore the spatial pattern of ectopic CYCB1;1 expression in bin4, we introduced the CYCB1;1:GUS construct (Colon-Carmona et al., 1999) into the bin4-1 background and examined the accumulation of CYCB1;1 transcripts. In the wild type, CYCB1;1 was predominantly expressed in proliferating tissues, and very little GUS signal was detected in mature tissues (Colon-Carmona et al., 1999) (Figures 7B, 7D, 7F, 7H, and 7J). We detected similar, or slightly reduced, levels of CYCB1;1 expression in the bin4 root meristems (Figure 7C), suggesting that bin4 is not greatly impaired in G2/M progression during the mitotic cell cycle. Unexpectedly, however, we found strong CYCB1;1 accumulation in nondividing cells, including those in light- and dark-grown hypocotyls (Figures 7E and 7G, respectively), cotyledons (Figure 7I), the mature part of roots (Figure 7K), leaf trichomes (Figure 7L), and leaf marginal cells

Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis

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Gene expression

In addition, our real-time RT-PCR analysis revealed that several DSB-inducible genes, including ... At PARP2 ... were significantly upregulated in bin4

Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis

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Gene expression

CYCB2;1 ... does not appear to differ between the wild type and bin4

Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis

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Gene expression

We further investigated the expression pattern of At PARP2 by introducing the pPARP2:GUS construct (Babiychuk et al., 1998) into the bin4-1 background. The histochemical analysis revealed that the At PARP2 gene is normally expressed at an undetectable level (Figures 5B, 5F, 5H, 5J, and 5L). To our surprise, however, we found that the bin4 mutation induces the expression of At PARP2 predominantly in postmeristematic cells ... In addition, we detected similar ectopic expression of At PARP2 in other postproliferating tissues, including light- and dark-grown hypocotyls (Figures 5G and 5I, respectively), cotyledons (Figure 5K), mature part of roots (Figure 5M), leaf trichomes (Figure 5N), and leaf marginal cells (Figure 5O), in bin4. These results suggest that the induced transcriptional activation of DNA damage response genes is restricted to the nonproliferating cells that have undergone several endocycles

Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis

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Gene expression

BIN4 promoter is active in endoreduplicating cells, including ... vascular cells (Figure 4C), elongating root cells (Figures 4E and 4H), and developing leaf trichomes

Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis

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Gene expression

KLU is expressed ... at the base of developing leaves

Anastasiou E, Kenz S, Gerstung M, MacLean D, Timmer J, Fleck C, Lenhard M - Control of plant organ size by KLUH/CYP78A5-dependent intercellular signaling

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Gene expression

To localize KLU protein, we expressed a KLU-vYFP fusion protein under the control of the KLU promoter. The fusion protein fully complemented the klu mutant phenotype, demonstrating its functionality (Figure S2I). Intracellularly, vYFP fluorescence was detected in a ring around the nucleus and in a mesh-like pattern in the cytoplasm (Figure 4I), which is typical for ER-associated proteins

Anastasiou E, Kenz S, Gerstung M, MacLean D, Timmer J, Fleck C, Lenhard M - Control of plant organ size by KLUH/CYP78A5-dependent intercellular signaling

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Gene expression

When comparing the regions of KLU expression and cell division in young leaves (Figures 4A and 4B) and in petals at the stage that is most sensitive to KLU activity (Figures 4E′ and 4F′; cf. above), it is apparent that KLU is expressed outside the region of proliferation. Of note, KLU expression is still detected when cells have already ceased to proliferate (Figures 4E″ and 4F″), suggesting that it is not the downregulation of KLU expression that causes the eventual arrest of proliferation

Anastasiou E, Kenz S, Gerstung M, MacLean D, Timmer J, Fleck C, Lenhard M - Control of plant organ size by KLUH/CYP78A5-dependent intercellular signaling

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Gene expression

KLU is expressed ... at the boundary of the shoot and lateral organs

Anastasiou E, Kenz S, Gerstung M, MacLean D, Timmer J, Fleck C, Lenhard M - Control of plant organ size by KLUH/CYP78A5-dependent intercellular signaling

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Gene expression

KLU is expressed on the flanks of the shoot meristem

Anastasiou E, Kenz S, Gerstung M, MacLean D, Timmer J, Fleck C, Lenhard M - Control of plant organ size by KLUH/CYP78A5-dependent intercellular signaling

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Gene expression

We generated C-terminal and N-terminal GFP translational fusion genomic rescue constructs: pSEU::GFP:SEU and pSEU::SEU:GFP ... For all transformants, GFP florescence was detected in all tissues examined ... vegetative ... SAM

Azhakanandam S, Nole-Wilson S, Bao F, Franks RG - SEUSS and AINTEGUMENTA mediate patterning and ovule initiation during gynoecium medial domain development

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Gene expression

We generated C-terminal and N-terminal GFP translational fusion genomic rescue constructs: pSEU::GFP:SEU and pSEU::SEU:GFP ... For all transformants, GFP florescence was detected in all tissues examined ... young leaf

Azhakanandam S, Nole-Wilson S, Bao F, Franks RG - SEUSS and AINTEGUMENTA mediate patterning and ovule initiation during gynoecium medial domain development

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Gene expression

AtEXP3 was induced by IAA only ... in wild type seedlings

Kwon YR, Lee HJ, Kim KH, Hong SW, Lee SJ, Lee H - Ectopic expression of Expansin3 or Expansinbeta1 causes enhanced hormone and salt stress sensitivity in Arabidopsis

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Gene expression

AtEXPβ1 was not induced by IAA in wild type seedlings

Kwon YR, Lee HJ, Kim KH, Hong SW, Lee SJ, Lee H - Ectopic expression of Expansin3 or Expansinbeta1 causes enhanced hormone and salt stress sensitivity in Arabidopsis

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Gene expression

AtEXPβ1 was induced by ACC only in wild type seedlings

Kwon YR, Lee HJ, Kim KH, Hong SW, Lee SJ, Lee H - Ectopic expression of Expansin3 or Expansinbeta1 causes enhanced hormone and salt stress sensitivity in Arabidopsis

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Gene expression

A DA1 promoter::GUS fusion (pDA1::GUS) was expressed most strongly during the early stages of leaf, petal, integument, and embryo formation (Fig. 4D–H) and at much reduced levels later in petal and leaf development, consistent with its role in controlling the period of cell proliferation

Li Y, Zheng L, Corke F, Smith C, Bevan MW - Control of final seed and organ size by the DA1 gene family in Arabidopsis thaliana

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Gene expression

Microarray data showed that DA1 is expressed at higher levels in ABA-treated seedlings (Li et al. 2006), and further experiments established that DA1 is slowly induced by ABA (Fig. 4L) and not by other growth regulators such as jasmonic acid, auxin, cytokinin, brassinosteroids, gibberellins, or glucose

Li Y, Zheng L, Corke F, Smith C, Bevan MW - Control of final seed and organ size by the DA1 gene family in Arabidopsis thaliana

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Gene expression

To assess the subcellular distribution of UBP15, constructs containing either 35S:GFP-UBP15 or 35S:UBP15-GFP were transformed into onion epidermal cells. Transient expression showed that the fusion proteins were ubiquitously present in both the cytosol and nucleus

Liu Y, Wang F, Zhang H, He H, Ma L, Deng XW - Functional characterization of the Arabidopsis ubiquitin-specific protease gene family reveals specific role and redundancy of individual members in development

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Gene expression

To assess the subcellular distribution of UBP15, constructs containing either 35S:GFP-UBP15 or 35S:UBP15-GFP were transformed into onion epidermal cells. Transient expression showed that the fusion proteins were ubiquitously present in both the cytosol and nucleus

Liu Y, Wang F, Zhang H, He H, Ma L, Deng XW - Functional characterization of the Arabidopsis ubiquitin-specific protease gene family reveals specific role and redundancy of individual members in development

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Gene expression

in ubp15-1 ... ICK1 was up-regulated

Liu Y, Wang F, Zhang H, He H, Ma L, Deng XW - Functional characterization of the Arabidopsis ubiquitin-specific protease gene family reveals specific role and redundancy of individual members in development

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Gene expression

in ubp15-1 ... Expression of the CAL gene ... was slightly increased (by 1.98-fold)

Liu Y, Wang F, Zhang H, He H, Ma L, Deng XW - Functional characterization of the Arabidopsis ubiquitin-specific protease gene family reveals specific role and redundancy of individual members in development

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Gene expression

in ubp15-1 ... Cyclin-like F-box domain-containing protein was down-regulated

Liu Y, Wang F, Zhang H, He H, Ma L, Deng XW - Functional characterization of the Arabidopsis ubiquitin-specific protease gene family reveals specific role and redundancy of individual members in development

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Gene expression

in ubp15-1 ... expression of MAF5 ... decreased by 0.31-fold

Liu Y, Wang F, Zhang H, He H, Ma L, Deng XW - Functional characterization of the Arabidopsis ubiquitin-specific protease gene family reveals specific role and redundancy of individual members in development

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Gene expression

the mRNA level of UBP15 was higher in rosette leaves and inflorescences than in other organs ... We further examined the spatial expression pattern of UBP15 within a rosette leaf at various different developmental stages. Interestingly, the UBP15 mRNA level increased from the early to late leaf stages, with higher expression in the leaf margin in the late stage

Liu Y, Wang F, Zhang H, He H, Ma L, Deng XW - Functional characterization of the Arabidopsis ubiquitin-specific protease gene family reveals specific role and redundancy of individual members in development

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Gene expression

We found that there were substantially fewer Histone H4–expressing cells in the ProSPL9:rSPL9 shoot apical meristem

Wang JW, Schwab R, Czech B, Mica E, Weigel D - Dual effects of miR156-targeted SPL genes and CYP78A5/KLUH on plastochron length and organ size in Arabidopsis thaliana

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Gene expression

There was no significant change of CYP78A5 transcript levels in ... Pro35S:MIR156f ... plants

Wang JW, Schwab R, Czech B, Mica E, Weigel D - Dual effects of miR156-targeted SPL genes and CYP78A5/KLUH on plastochron length and organ size in Arabidopsis thaliana

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Gene expression

CYP78A5/KLUH ... expressed at the periphery of the meristem proper

Wang JW, Schwab R, Czech B, Mica E, Weigel D - Dual effects of miR156-targeted SPL genes and CYP78A5/KLUH on plastochron length and organ size in Arabidopsis thaliana

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Gene expression

SPL9 mRNA was found in leaf anlagen and in developing leaf primordia

Wang JW, Schwab R, Czech B, Mica E, Weigel D - Dual effects of miR156-targeted SPL genes and CYP78A5/KLUH on plastochron length and organ size in Arabidopsis thaliana

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Gene expression

direct analysis of miR156 expression by in situ hybridization, which revealed that miR156 accumulated ... in ... leaf primordia

Wang JW, Schwab R, Czech B, Mica E, Weigel D - Dual effects of miR156-targeted SPL genes and CYP78A5/KLUH on plastochron length and organ size in Arabidopsis thaliana

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Gene expression

SPL9 levels were unaffected by cyp78a5

Wang JW, Schwab R, Czech B, Mica E, Weigel D - Dual effects of miR156-targeted SPL genes and CYP78A5/KLUH on plastochron length and organ size in Arabidopsis thaliana

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Gene expression

As young leaves grew, SPL9 expression became gradually re- stricted to vascular tissues

Wang JW, Schwab R, Czech B, Mica E, Weigel D - Dual effects of miR156-targeted SPL genes and CYP78A5/KLUH on plastochron length and organ size in Arabidopsis thaliana

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Gene expression

direct analysis of miR156 expression by in situ hybridization, which revealed that miR156 accumulated ... in ... shoot apical meristem proper

Wang JW, Schwab R, Czech B, Mica E, Weigel D - Dual effects of miR156-targeted SPL genes and CYP78A5/KLUH on plastochron length and organ size in Arabidopsis thaliana

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Gene expression

In Pro35S:MIR156f plants, SPL9 transcripts were strongly reduced

Wang JW, Schwab R, Czech B, Mica E, Weigel D - Dual effects of miR156-targeted SPL genes and CYP78A5/KLUH on plastochron length and organ size in Arabidopsis thaliana

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Gene expression

we did not detect any SPL9 transcripts in the central part of the meristem

Wang JW, Schwab R, Czech B, Mica E, Weigel D - Dual effects of miR156-targeted SPL genes and CYP78A5/KLUH on plastochron length and organ size in Arabidopsis thaliana

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Gene expression

There was no significant change of CYP78A5 transcript levels in ... ProSPL9:rSPL9 plants

Wang JW, Schwab R, Czech B, Mica E, Weigel D - Dual effects of miR156-targeted SPL genes and CYP78A5/KLUH on plastochron length and organ size in Arabidopsis thaliana

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Gene expression

We therefore analyzed SPL9 expression in two mutants with reduced miR156 levels, se-1 and ago1-27. Compared with the wild type, SPL9 RNA levels were substantially elevated in both mutants. However, the overall pattern of expression was similar to that seen in the wild type

Wang JW, Schwab R, Czech B, Mica E, Weigel D - Dual effects of miR156-targeted SPL genes and CYP78A5/KLUH on plastochron length and organ size in Arabidopsis thaliana

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Gene expression

We therefore analyzed SPL9 expression in two mutants with reduced miR156 levels, se-1 and ago1-27. Compared with the wild type, SPL9 RNA levels were substantially elevated in both mutants. However, the overall pattern of expression was similar to that seen in the wild type

Wang JW, Schwab R, Czech B, Mica E, Weigel D - Dual effects of miR156-targeted SPL genes and CYP78A5/KLUH on plastochron length and organ size in Arabidopsis thaliana

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Gene expression

we employed transgenic plants in which miR156 activity is specifically reduced by con- stitutive expression of a target mimic (Franco-Zorrilla et al., 2007). SPL9 mRNA expression was changed in these Pro35S: MIM156 plants in a similar manner as in se-1 and ago1-27 plants

Wang JW, Schwab R, Czech B, Mica E, Weigel D - Dual effects of miR156-targeted SPL genes and CYP78A5/KLUH on plastochron length and organ size in Arabidopsis thaliana

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Gene expression

CYP78A5/KLUH ... expressed ... at the base of new leaf primordia

Wang JW, Schwab R, Czech B, Mica E, Weigel D - Dual effects of miR156-targeted SPL genes and CYP78A5/KLUH on plastochron length and organ size in Arabidopsis thaliana

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Gene expression

Col CPL2pro:CPL2-GUS seedlings ... In shoots, GUS activity was particularly strong in ... shoot meristems

Ueda A, Li P, Feng Y, Vikram M, Kim S, Kang CH, Kang JS, Bahk JD, Lee SY, Fukuhara T, Staswick PE, Pepper AE, Koiwa H - The Arabidopsis thaliana carboxyl-terminal domain phosphatase-like 2 regulates plant growth, stress and auxin responses

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Gene expression

In Col CPL2pro:CPL2-GUS seedlings, CPL2-GUS expression was observed throughout the plants (Fig. 2b). In later stages, GUS activity was associated with tissues undergoing cell division and expansion. In shoots, GUS activity was particularly strong in expanding leaves

Ueda A, Li P, Feng Y, Vikram M, Kim S, Kang CH, Kang JS, Bahk JD, Lee SY, Fukuhara T, Staswick PE, Pepper AE, Koiwa H - The Arabidopsis thaliana carboxyl-terminal domain phosphatase-like 2 regulates plant growth, stress and auxin responses

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Gene expression

In order to determine the level of auxin responsiveness of whole plants, DR5-GUS expressions in wild type and cpl2 was determined using a histochemical GUS assay. GUS activity in untreated cpl2-2 leaves was very low, whereas its expression level in the roots was similar to wild type (Fig. 8a). Consistently, root growth responses and lateral root development of wild type and cpl2 alleles to exogenously supplied auxin were indistinguishable

Ueda A, Li P, Feng Y, Vikram M, Kim S, Kang CH, Kang JS, Bahk JD, Lee SY, Fukuhara T, Staswick PE, Pepper AE, Koiwa H - The Arabidopsis thaliana carboxyl-terminal domain phosphatase-like 2 regulates plant growth, stress and auxin responses

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Gene expression

Unlike fry2-1, RD29a-LUC expression in cpl2-2 was specifically hyper-responsive to high NaCl treatment (Fig. 4a), but its cold and ABA induction was not as pronounced as in fry2-1

Ueda A, Li P, Feng Y, Vikram M, Kim S, Kang CH, Kang JS, Bahk JD, Lee SY, Fukuhara T, Staswick PE, Pepper AE, Koiwa H - The Arabidopsis thaliana carboxyl-terminal domain phosphatase-like 2 regulates plant growth, stress and auxin responses

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Gene expression

Col CPL2pro:CPL2-GUS seedlings ... cotyledons were stained only in ... petioles

Ueda A, Li P, Feng Y, Vikram M, Kim S, Kang CH, Kang JS, Bahk JD, Lee SY, Fukuhara T, Staswick PE, Pepper AE, Koiwa H - The Arabidopsis thaliana carboxyl-terminal domain phosphatase-like 2 regulates plant growth, stress and auxin responses

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Gene expression

Basal expression level of DR5-GUS in cpl2-2 seedlings was 28% of wild type seedlings (Fig. 6). This is consistent with the data obtained with GUS activity based on MUG assay (data not shown).

Ueda A, Li P, Feng Y, Vikram M, Kim S, Kang CH, Kang JS, Bahk JD, Lee SY, Fukuhara T, Staswick PE, Pepper AE, Koiwa H - The Arabidopsis thaliana carboxyl-terminal domain phosphatase-like 2 regulates plant growth, stress and auxin responses

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Gene expression

basal expression levels of IAA1 ... were decreased in cpl2-2 mutant plants

Ueda A, Li P, Feng Y, Vikram M, Kim S, Kang CH, Kang JS, Bahk JD, Lee SY, Fukuhara T, Staswick PE, Pepper AE, Koiwa H - The Arabidopsis thaliana carboxyl-terminal domain phosphatase-like 2 regulates plant growth, stress and auxin responses

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Gene expression

basal expression levels of ... IAA19 were decreased in cpl2-2 mutant plants

Ueda A, Li P, Feng Y, Vikram M, Kim S, Kang CH, Kang JS, Bahk JD, Lee SY, Fukuhara T, Staswick PE, Pepper AE, Koiwa H - The Arabidopsis thaliana carboxyl-terminal domain phosphatase-like 2 regulates plant growth, stress and auxin responses

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Gene expression

Col CPL2pro:CPL2-GUS seedlings ... cotyledons were stained only in vein tissues

Ueda A, Li P, Feng Y, Vikram M, Kim S, Kang CH, Kang JS, Bahk JD, Lee SY, Fukuhara T, Staswick PE, Pepper AE, Koiwa H - The Arabidopsis thaliana carboxyl-terminal domain phosphatase-like 2 regulates plant growth, stress and auxin responses

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Gene expression

HOG1 OE ... lines ... ARR5 ... 3- to 9-fold decrease in the expression levels

Godge MR, Kumar D, Kumar PP - Arabidopsis HOG1 gene and its petunia homolog PETCBP act as key regulators of yield parameters

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Gene expression

We performed qRT-PCR analysis with RNA from seedlings of ... HOG1 ... AS lines ... The transcript levels of KNAT1 ... were significantly upregulated by 6- to 8-fold even without BA treatment

Godge MR, Kumar D, Kumar PP - Arabidopsis HOG1 gene and its petunia homolog PETCBP act as key regulators of yield parameters

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Gene expression

HOG1 ... AS lines ... ARR4 ... higher level (4- to 9-fold increase)

Godge MR, Kumar D, Kumar PP - Arabidopsis HOG1 gene and its petunia homolog PETCBP act as key regulators of yield parameters

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Gene expression

HOG1 OE ... lines ... ARR6 ... 3- to 9-fold decrease in the expression levels

Godge MR, Kumar D, Kumar PP - Arabidopsis HOG1 gene and its petunia homolog PETCBP act as key regulators of yield parameters

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Gene expression

ARR5 ... were upregulated within minutes after a single cytokinin pulse treatment

Godge MR, Kumar D, Kumar PP - Arabidopsis HOG1 gene and its petunia homolog PETCBP act as key regulators of yield parameters

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Gene expression

HOG1 OE ... lines ... ARR4 ... 3- to 9-fold decrease in the expression levels

Godge MR, Kumar D, Kumar PP - Arabidopsis HOG1 gene and its petunia homolog PETCBP act as key regulators of yield parameters

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Gene expression

HOG1 ... AS lines ... ARR5 ... higher level (4- to 9-fold increase)

Godge MR, Kumar D, Kumar PP - Arabidopsis HOG1 gene and its petunia homolog PETCBP act as key regulators of yield parameters

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Gene expression

Expression analysis of different plant parts indicated that HOG1 (Fig. 2d) and HOG2 (Fig. 2a) are constitutively expressed in Arabidopsis, with relatively higher levels in the leaves and inflorescence stem

Godge MR, Kumar D, Kumar PP - Arabidopsis HOG1 gene and its petunia homolog PETCBP act as key regulators of yield parameters

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Gene expression

HOG1 ... AS lines ... ARR6 ... higher level (4- to 9-fold increase)

Godge MR, Kumar D, Kumar PP - Arabidopsis HOG1 gene and its petunia homolog PETCBP act as key regulators of yield parameters

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Gene expression

Application of BA caused a dose-dependent increase of ... STM transcripts

Godge MR, Kumar D, Kumar PP - Arabidopsis HOG1 gene and its petunia homolog PETCBP act as key regulators of yield parameters

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Gene expression

We performed qRT-PCR analysis with RNA from seedlings of ... HOG1 ... AS lines ... The transcript levels of ... STM were significantly upregulated by 6- to 8-fold even without BA treatment

Godge MR, Kumar D, Kumar PP - Arabidopsis HOG1 gene and its petunia homolog PETCBP act as key regulators of yield parameters

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Gene expression

ARR6 ... were upregulated within minutes after a single cytokinin pulse treatment

Godge MR, Kumar D, Kumar PP - Arabidopsis HOG1 gene and its petunia homolog PETCBP act as key regulators of yield parameters

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Gene expression

ARR4 ... were upregulated within minutes after a single cytokinin pulse treatment

Godge MR, Kumar D, Kumar PP - Arabidopsis HOG1 gene and its petunia homolog PETCBP act as key regulators of yield parameters

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Gene expression

Expression analysis of different plant parts indicated that HOG1 (Fig. 2d) and HOG2 (Fig. 2a) are constitutively expressed in Arabidopsis, with relatively higher levels in the leaves and inflorescence stem

Godge MR, Kumar D, Kumar PP - Arabidopsis HOG1 gene and its petunia homolog PETCBP act as key regulators of yield parameters

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Gene expression

Application of BA caused a dose-dependent increase of KNAT1

Godge MR, Kumar D, Kumar PP - Arabidopsis HOG1 gene and its petunia homolog PETCBP act as key regulators of yield parameters

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Gene expression

HOG1 OE ... lines ... showed 4- to 7-fold decrease of ... STM transcripts

Godge MR, Kumar D, Kumar PP - Arabidopsis HOG1 gene and its petunia homolog PETCBP act as key regulators of yield parameters

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Gene expression

HOG1 OE ... lines ... showed 4- to 7-fold decrease of ... KNAT1

Godge MR, Kumar D, Kumar PP - Arabidopsis HOG1 gene and its petunia homolog PETCBP act as key regulators of yield parameters

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Gene expression

For IAA28 overexpressing lines, the IAA13 transcription level was not altered

Falkenberg B, Witt I, Zanor MI, Steinhauser D, Mueller-Roeber B, Hesse H, Hoefgen R - Transcription factors relevant to auxin signalling coordinate broad-spectrum metabolic shifts including sulphur metabolism

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Gene expression

For IAA28 overexpressing lines ... the ARF1-BP transcript was down-regulated by up to 88%

Falkenberg B, Witt I, Zanor MI, Steinhauser D, Mueller-Roeber B, Hesse H, Hoefgen R - Transcription factors relevant to auxin signalling coordinate broad-spectrum metabolic shifts including sulphur metabolism

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Gene expression

IAA28 ... up-regulated during sulphur starvation in Arabidopsis thaliana seedlings

Falkenberg B, Witt I, Zanor MI, Steinhauser D, Mueller-Roeber B, Hesse H, Hoefgen R - Transcription factors relevant to auxin signalling coordinate broad-spectrum metabolic shifts including sulphur metabolism

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Gene expression

In ARF1-BP overexpressing lines ... IAA13 transcript accumulated ... 2-fold

Falkenberg B, Witt I, Zanor MI, Steinhauser D, Mueller-Roeber B, Hesse H, Hoefgen R - Transcription factors relevant to auxin signalling coordinate broad-spectrum metabolic shifts including sulphur metabolism

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Gene expression

In IAA13 overexpressing lines ... ARF1-BP transcript was reduced by 86%

Falkenberg B, Witt I, Zanor MI, Steinhauser D, Mueller-Roeber B, Hesse H, Hoefgen R - Transcription factors relevant to auxin signalling coordinate broad-spectrum metabolic shifts including sulphur metabolism

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Gene expression

IAA13 ... up-regulated during sulphur starvation in Arabidopsis thaliana seedlings

Falkenberg B, Witt I, Zanor MI, Steinhauser D, Mueller-Roeber B, Hesse H, Hoefgen R - Transcription factors relevant to auxin signalling coordinate broad-spectrum metabolic shifts including sulphur metabolism

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Gene expression

In ARF1-BP overexpressing lines ... IAA28 ... transcript accumulated 3-fold

Falkenberg B, Witt I, Zanor MI, Steinhauser D, Mueller-Roeber B, Hesse H, Hoefgen R - Transcription factors relevant to auxin signalling coordinate broad-spectrum metabolic shifts including sulphur metabolism

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Gene expression

ARF2 ... up-regulated during sulphur starvation in Arabidopsis thaliana seedlings

Falkenberg B, Witt I, Zanor MI, Steinhauser D, Mueller-Roeber B, Hesse H, Hoefgen R - Transcription factors relevant to auxin signalling coordinate broad-spectrum metabolic shifts including sulphur metabolism

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Gene expression

In seedlings expressing the GUS protein under the control of the DOT1 promoter, expression was detected in emerging leaf primordia and young leaves (Figure S5

Petricka JJ, Clay NK, Nelson TM - Vein patterning screens and the defectively organized tributaries mutants in Arabidopsis thaliana

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Gene expression

A DOT3 promoter fused to GUS was detected in emerging leaf primordia (Figure S5

Petricka JJ, Clay NK, Nelson TM - Vein patterning screens and the defectively organized tributaries mutants in Arabidopsis thaliana

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Gene expression

Expression of a DOT2 promoter-GUS fusion can be seen in young leaves (Figure S5

Petricka JJ, Clay NK, Nelson TM - Vein patterning screens and the defectively organized tributaries mutants in Arabidopsis thaliana

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Gene expression

Very weak expression in leaves was detected in DOT4 promoter GUS lines (Figure S5

Petricka JJ, Clay NK, Nelson TM - Vein patterning screens and the defectively organized tributaries mutants in Arabidopsis thaliana

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Gene expression

genes induced by threefold or more in BA-treated wild-type shoots ... includes type A ARRs, the cytokinin receptor AHK4

Argyros RD, Mathews DE, Chiang YH, Palmer CM, Thibault DM, Etheridge N, Argyros DA, Mason MG, Kieber JJ, Schaller GE - Type B response regulators of Arabidopsis play key roles in cytokinin signaling and plant development

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Gene expression

AHK4 ... in the arr1-3 arr10-5 arr12-1 mutant ... basal expression level was reduced compared with that in the wild type, indicating a reduced response to endogenous cytokinin in the mutant

Argyros RD, Mathews DE, Chiang YH, Palmer CM, Thibault DM, Etheridge N, Argyros DA, Mason MG, Kieber JJ, Schaller GE - Type B response regulators of Arabidopsis play key roles in cytokinin signaling and plant development

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Gene expression

ARR15 ... in the arr1-3 arr10-5 arr12-1 mutant ... basal expression level was reduced compared with that in the wild type, indicating a reduced response to endogenous cytokinin in the mutant

Argyros RD, Mathews DE, Chiang YH, Palmer CM, Thibault DM, Etheridge N, Argyros DA, Mason MG, Kieber JJ, Schaller GE - Type B response regulators of Arabidopsis play key roles in cytokinin signaling and plant development

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Gene expression

CKX4 in the arr1-3 arr10-5 arr12-1 mutant ... basal expression level was reduced compared with that in the wild type, indicating a reduced response to endogenous cytokinin in the mutant

Argyros RD, Mathews DE, Chiang YH, Palmer CM, Thibault DM, Etheridge N, Argyros DA, Mason MG, Kieber JJ, Schaller GE - Type B response regulators of Arabidopsis play key roles in cytokinin signaling and plant development

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Gene expression

ARR7 ... the arr1-3 arr10-5 arr12-1 mutant ... basal expression level was reduced compared with that in the wild type, indicating a reduced response to endogenous cytokinin in the mutant

Argyros RD, Mathews DE, Chiang YH, Palmer CM, Thibault DM, Etheridge N, Argyros DA, Mason MG, Kieber JJ, Schaller GE - Type B response regulators of Arabidopsis play key roles in cytokinin signaling and plant development

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Gene expression

ARR5 ... in the arr1-3 arr10-5 arr12-1 mutant ... basal expression level was reduced compared with that in the wild type, indicating a reduced response to endogenous cytokinin in the mutant

Argyros RD, Mathews DE, Chiang YH, Palmer CM, Thibault DM, Etheridge N, Argyros DA, Mason MG, Kieber JJ, Schaller GE - Type B response regulators of Arabidopsis play key roles in cytokinin signaling and plant development

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Gene expression

genes induced by threefold or more in BA-treated wild-type shoots ... includes ... cytokinin oxidase CKX4

Argyros RD, Mathews DE, Chiang YH, Palmer CM, Thibault DM, Etheridge N, Argyros DA, Mason MG, Kieber JJ, Schaller GE - Type B response regulators of Arabidopsis play key roles in cytokinin signaling and plant development

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Gene expression

The arr1-3 arr10-5 arr12-1 mutant exhibited a severely abrogated response to BA, with only one of these 71 genes (ARR5) exhibiting a greater than threefold induction upon treatment with BA

Argyros RD, Mathews DE, Chiang YH, Palmer CM, Thibault DM, Etheridge N, Argyros DA, Mason MG, Kieber JJ, Schaller GE - Type B response regulators of Arabidopsis play key roles in cytokinin signaling and plant development

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Gene expression

Consistent with the decreased cell division in the arr1-3 arr10-5 arr12-1 mutant, real-time RT-PCR revealed a significant difference (P < 0.001, t test) in the basal levels of CycD3;1 expression, which is 43% lower in the mutant compared with the wild type

Argyros RD, Mathews DE, Chiang YH, Palmer CM, Thibault DM, Etheridge N, Argyros DA, Mason MG, Kieber JJ, Schaller GE - Type B response regulators of Arabidopsis play key roles in cytokinin signaling and plant development

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Gene expression

most suppressed gene in our original analysis of jaw-D ... plants ... TCP4

Schommer C, Palatnik JF, Aggarwal P, Chételat A, Cubas P, Farmer EE, Nath U, Weigel D - Control of jasmonate biosynthesis and senescence by miR319 targets

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Gene expression

The expression of ... LOX4 ... is ... increased in rTCP4 plants

Schommer C, Palatnik JF, Aggarwal P, Chételat A, Cubas P, Farmer EE, Nath U, Weigel D - Control of jasmonate biosynthesis and senescence by miR319 targets

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Gene expression

other lipoxygenases that are predicted to be chloroplast-localized ... LOX4

Schommer C, Palatnik JF, Aggarwal P, Chételat A, Cubas P, Farmer EE, Nath U, Weigel D - Control of jasmonate biosynthesis and senescence by miR319 targets

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Gene expression

The expression of LOX3 ... is ... increased in rTCP4 plants

Schommer C, Palatnik JF, Aggarwal P, Chételat A, Cubas P, Farmer EE, Nath U, Weigel D - Control of jasmonate biosynthesis and senescence by miR319 targets

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Gene expression

LOX2:GUS ... wild-type reporter had strong GUS activity throughout leaves, similar to what has been reported

Schommer C, Palatnik JF, Aggarwal P, Chételat A, Cubas P, Farmer EE, Nath U, Weigel D - Control of jasmonate biosynthesis and senescence by miR319 targets

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Gene expression

LOX2 ... chloroplast-localized

Schommer C, Palatnik JF, Aggarwal P, Chételat A, Cubas P, Farmer EE, Nath U, Weigel D - Control of jasmonate biosynthesis and senescence by miR319 targets

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Gene expression

other lipoxygenases that are predicted to be chloroplast-localized ... LOX3

Schommer C, Palatnik JF, Aggarwal P, Chételat A, Cubas P, Farmer EE, Nath U, Weigel D - Control of jasmonate biosynthesis and senescence by miR319 targets

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Gene expression

The expression of LOX3 ... is reduced in jaw-D plants

Schommer C, Palatnik JF, Aggarwal P, Chételat A, Cubas P, Farmer EE, Nath U, Weigel D - Control of jasmonate biosynthesis and senescence by miR319 targets

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Gene expression

LOX2 was the second most suppressed gene in our original analysis of jaw-D plants, after TCP4 itself

Schommer C, Palatnik JF, Aggarwal P, Chételat A, Cubas P, Farmer EE, Nath U, Weigel D - Control of jasmonate biosynthesis and senescence by miR319 targets

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Gene expression

LOX2:GUS ... wild-type reporter was less active in tcp2 tcp4 tcp10 triple mutants

Schommer C, Palatnik JF, Aggarwal P, Chételat A, Cubas P, Farmer EE, Nath U, Weigel D - Control of jasmonate biosynthesis and senescence by miR319 targets

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Gene expression

WRKY53 ... is induced more than 30 times in rTCP4:GFP plants, although it lacks TCP4 consensus binding motifs in its promoter

Schommer C, Palatnik JF, Aggarwal P, Chételat A, Cubas P, Farmer EE, Nath U, Weigel D - Control of jasmonate biosynthesis and senescence by miR319 targets

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Gene expression

other lipoxygenases that are predicted to be chloroplast-localized ... LOX6

Schommer C, Palatnik JF, Aggarwal P, Chételat A, Cubas P, Farmer EE, Nath U, Weigel D - Control of jasmonate biosynthesis and senescence by miR319 targets

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Gene expression

The expression of ... LOX4 ... is reduced in jaw-D plants

Schommer C, Palatnik JF, Aggarwal P, Chételat A, Cubas P, Farmer EE, Nath U, Weigel D - Control of jasmonate biosynthesis and senescence by miR319 targets

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Gene expression

AtCDT1a ... mRNA levels ... were downregulated in plants with higher levels of ABAP1 mRNA and protein

Masuda HP, Cabral LM, De Veylder L, Tanurdzic M, de Almeida Engler J, Geelen D, Inzé D, Martienssen RA, Ferreira PC, Hemerly AS - ABAP1 is a novel plant Armadillo BTB protein involved in DNA replication and transcription

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Gene expression

AtCDT1b mRNA levels ... were downregulated in plants with higher levels of ABAP1 mRNA and protein

Masuda HP, Cabral LM, De Veylder L, Tanurdzic M, de Almeida Engler J, Geelen D, Inzé D, Martienssen RA, Ferreira PC, Hemerly AS - ABAP1 is a novel plant Armadillo BTB protein involved in DNA replication and transcription

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Gene expression

confocal and fluorescent microscopy of Arabidopsis plants producing GFP::ABAP1 indicated that ABAP1 was exclusively located in the nucleus, homogeneously distributed or enriched in nuclear domains, and GFP::ABAP1 was absent in mitotic cells

Masuda HP, Cabral LM, De Veylder L, Tanurdzic M, de Almeida Engler J, Geelen D, Inzé D, Martienssen RA, Ferreira PC, Hemerly AS - ABAP1 is a novel plant Armadillo BTB protein involved in DNA replication and transcription

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Gene expression

AtCDT1b mRNA levels ... were upregulated in plants where ABAP1 mRNA and protein levels were diminished

Masuda HP, Cabral LM, De Veylder L, Tanurdzic M, de Almeida Engler J, Geelen D, Inzé D, Martienssen RA, Ferreira PC, Hemerly AS - ABAP1 is a novel plant Armadillo BTB protein involved in DNA replication and transcription

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Gene expression

GUS expression in the meristemoid and young stomatal cells illustrates ABAP1 expression during stomatal cell differentiation

Masuda HP, Cabral LM, De Veylder L, Tanurdzic M, de Almeida Engler J, Geelen D, Inzé D, Martienssen RA, Ferreira PC, Hemerly AS - ABAP1 is a novel plant Armadillo BTB protein involved in DNA replication and transcription

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Gene expression

Studies on localization of ABAP1 expression in plant tissues showed that ABAP1 was ... mainly expressed in ... young leaves ... As the leaf grew, GUS expression decreased in a tip-to-base gradient (Figure 2C and D). In agreement, ABAP1 mRNA levels were higher in developing leaves during proliferation stage (9-day-old plants) and rapidly declined as observed in leaves of 13 till 21-day-old plants

Masuda HP, Cabral LM, De Veylder L, Tanurdzic M, de Almeida Engler J, Geelen D, Inzé D, Martienssen RA, Ferreira PC, Hemerly AS - ABAP1 is a novel plant Armadillo BTB protein involved in DNA replication and transcription

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Gene expression

Studies on localization of ABAP1 expression in plant tissues showed that ABAP1 was ... mainly expressed in the shoot apex

Masuda HP, Cabral LM, De Veylder L, Tanurdzic M, de Almeida Engler J, Geelen D, Inzé D, Martienssen RA, Ferreira PC, Hemerly AS - ABAP1 is a novel plant Armadillo BTB protein involved in DNA replication and transcription

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Gene expression

AtCDT1a ... mRNA levels ... were upregulated in plants where ABAP1 mRNA and protein levels were diminished

Masuda HP, Cabral LM, De Veylder L, Tanurdzic M, de Almeida Engler J, Geelen D, Inzé D, Martienssen RA, Ferreira PC, Hemerly AS - ABAP1 is a novel plant Armadillo BTB protein involved in DNA replication and transcription

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Gene expression

Gene expression analyses of 9-day-old AtTCP24OE seedlings with real-time RT–PCR showed that AtCDT1a ... levels were downregulated in AtTCP24OE plants

Masuda HP, Cabral LM, De Veylder L, Tanurdzic M, de Almeida Engler J, Geelen D, Inzé D, Martienssen RA, Ferreira PC, Hemerly AS - ABAP1 is a novel plant Armadillo BTB protein involved in DNA replication and transcription

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Gene expression

Gene expression analyses of 9-day-old AtTCP24OE seedlings with real-time RT–PCR showed that ... AtCDT1b levels were downregulated in AtTCP24OE plants

Masuda HP, Cabral LM, De Veylder L, Tanurdzic M, de Almeida Engler J, Geelen D, Inzé D, Martienssen RA, Ferreira PC, Hemerly AS - ABAP1 is a novel plant Armadillo BTB protein involved in DNA replication and transcription

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Gene expression

in plants transformed with 35S:KAN1-GR, a construct that constitutively expresses a dexamethasone (DEX)-inducible form of KAN1. Semiquantitative RT-PCR analysis of 35S:KAN-GR plants grown continuously on DEX or on basal medium followed by DEX ... had no effect on the expression of AS1

Wu G, Lin WC, Huang T, Poethig RS, Springer PS, Kerstetter RA - KANADI1 regulates adaxial-abaxial polarity in Arabidopsis by directly repressing the transcription of ASYMMETRIC LEAVES2

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Gene expression

Plants mutant for both KAN1 and KAN2 expressed pAS2:GUS ubiquitously in cotyledons

Wu G, Lin WC, Huang T, Poethig RS, Springer PS, Kerstetter RA - KANADI1 regulates adaxial-abaxial polarity in Arabidopsis by directly repressing the transcription of ASYMMETRIC LEAVES2

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Gene expression

In as2–1, pKAN1:GUS expression extended to the adaxial side of young leaf primordia and persisted late in leaf development

Wu G, Lin WC, Huang T, Poethig RS, Springer PS, Kerstetter RA - KANADI1 regulates adaxial-abaxial polarity in Arabidopsis by directly repressing the transcription of ASYMMETRIC LEAVES2

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Gene expression

in plants transformed with 35S:KAN1-GR, a construct that constitutively expresses a dexamethasone (DEX)-inducible form of KAN1. Semiquantitative RT-PCR analysis of 35S:KAN-GR plants grown continuously on DEX or on basal medium followed by DEX ... had no effect on the expression of ... FIL1

Wu G, Lin WC, Huang T, Poethig RS, Springer PS, Kerstetter RA - KANADI1 regulates adaxial-abaxial polarity in Arabidopsis by directly repressing the transcription of ASYMMETRIC LEAVES2

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Gene expression

loss-of-function mutants of KAN1 ... had little effect on the expression of pAS2:GUS in cotyledons (Fig. 5B), but caused this reporter to be expressed on both the adaxial and abaxial sides of leaf primordia

Wu G, Lin WC, Huang T, Poethig RS, Springer PS, Kerstetter RA - KANADI1 regulates adaxial-abaxial polarity in Arabidopsis by directly repressing the transcription of ASYMMETRIC LEAVES2

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Gene expression

examined the expression of AS2 and several other polarity genes in plants transformed with 35S:KAN1-GR, a construct that constitutively expresses a dexamethasone (DEX)-inducible form of KAN1. Semiquantitative RT-PCR analysis of 35S:KAN-GR plants grown continuously on DEX or on basal medium followed by DEX ... had no effect on the expression of ... YAB3

Wu G, Lin WC, Huang T, Poethig RS, Springer PS, Kerstetter RA - KANADI1 regulates adaxial-abaxial polarity in Arabidopsis by directly repressing the transcription of ASYMMETRIC LEAVES2

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Gene expression

To examine the effect of the as2–5D mutation on the expression of AS2, we generated promoter–reporter constructs with ... mutated AS2 regulatory sequences fused to ßglucuronidase (GUS). For this purpose we used a 4.1-kb genomic fragment that is capable of rescuing the as2–1 phenotype when fused to an AS2 cDNA [supporting information (SI) Fig. S1]. Constructs with the ... mutated versions of this fragment containing either the as2–5D mutation (pas2–5D:GUS ... were introduced into plants by Agrobacterium-mediated transformation ... plants transformed with pas2–5D:GUS expressed GUS throughout the cotyledons

Wu G, Lin WC, Huang T, Poethig RS, Springer PS, Kerstetter RA - KANADI1 regulates adaxial-abaxial polarity in Arabidopsis by directly repressing the transcription of ASYMMETRIC LEAVES2

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Gene expression

Plants mutant for both KAN1 and KAN2 expressed pAS2:GUS ubiquitously in ... leaf primordia

Wu G, Lin WC, Huang T, Poethig RS, Springer PS, Kerstetter RA - KANADI1 regulates adaxial-abaxial polarity in Arabidopsis by directly repressing the transcription of ASYMMETRIC LEAVES2

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Gene expression

To examine the effect of the as2–5D mutation on the expression of AS2, we generated promoter–reporter constructs with ... mutated AS2 regulatory sequences fused to ßglucuronidase (GUS). For this purpose we used a 4.1-kb genomic fragment that is capable of rescuing the as2–1 phenotype when fused to an AS2 cDNA [supporting information (SI) Fig. S1]. Constructs with the ... mutated versions of this fragment containing either the as2–5D mutation (pas2–5D:GUS ... were introduced into plants by Agrobacterium-mediated transformation ... plants transformed with pas2–5D:GUS expressed GUS throughout the cotyledons, leaf primordia, and the hypocotyl

Wu G, Lin WC, Huang T, Poethig RS, Springer PS, Kerstetter RA - KANADI1 regulates adaxial-abaxial polarity in Arabidopsis by directly repressing the transcription of ASYMMETRIC LEAVES2

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Gene expression

Consistent with their subtle morphological phenotype, mutations in KAN2 had no apparent effect on pAS2:GUS expression

Wu G, Lin WC, Huang T, Poethig RS, Springer PS, Kerstetter RA - KANADI1 regulates adaxial-abaxial polarity in Arabidopsis by directly repressing the transcription of ASYMMETRIC LEAVES2

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Gene expression

in as2–5D plants pKAN1:GUS expression was excluded from leaf primordia

Wu G, Lin WC, Huang T, Poethig RS, Springer PS, Kerstetter RA - KANADI1 regulates adaxial-abaxial polarity in Arabidopsis by directly repressing the transcription of ASYMMETRIC LEAVES2

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Gene expression

in plants transformed with 35S:KAN1-GR, a construct that constitutively expresses a dexamethasone (DEX)-inducible form of KAN1. Semiquantitative RT-PCR analysis of 35S:KAN-GR plants grown continuously on DEX or on basal medium followed by DEX revealed that DEX reduced the amount of AS2 mRNA

Wu G, Lin WC, Huang T, Poethig RS, Springer PS, Kerstetter RA - KANADI1 regulates adaxial-abaxial polarity in Arabidopsis by directly repressing the transcription of ASYMMETRIC LEAVES2

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Gene expression

using in situ hybridization to examine the expression of WUS ... Among 14-day-old emb1611-2 seedlings, 50% showed WUS expression in a reduced domain ... in the SAM

Leasure CD, Fiume E, Fletcher JC - The essential gene EMB1611 maintains shoot apical meristem function during Arabidopsis development

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Gene expression

using in situ hybridization to ... examine the expression of WUS ... Among 7-day-old emb1611-2 seedlings, 50% had a normal WUS expression domain ... in the SAM

Leasure CD, Fiume E, Fletcher JC - The essential gene EMB1611 maintains shoot apical meristem function during Arabidopsis development

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Gene expression

using in situ hybridization to examine the expression of WUS ... Among 7-day-old emb1611-2 seedlings ... 50% displayed no WUS expression in the SAM

Leasure CD, Fiume E, Fletcher JC - The essential gene EMB1611 maintains shoot apical meristem function during Arabidopsis development

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Gene expression

Some 13-day-old emb1611-2 seedlings showed a normal pattern of pSTM::uidA activity

Leasure CD, Fiume E, Fletcher JC - The essential gene EMB1611 maintains shoot apical meristem function during Arabidopsis development

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Gene expression

The pattern of pSTM::uidA activity in the periphery of the meristem was largely unaltered in 7-day-old emb1611-2 seedlings ... compared with wild-type seedlings

Leasure CD, Fiume E, Fletcher JC - The essential gene EMB1611 maintains shoot apical meristem function during Arabidopsis development

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Gene expression

Some 13-day-old emb1611-2 seedlings ... displayed pSTM::uidA activity throughout the SAM

Leasure CD, Fiume E, Fletcher JC - The essential gene EMB1611 maintains shoot apical meristem function during Arabidopsis development

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Gene expression

using in situ hybridization to examine the expression of WUS ... Among 14-day-old emb1611-2 seedlings ... 50% displayed no WUS expression in the SAM

Leasure CD, Fiume E, Fletcher JC - The essential gene EMB1611 maintains shoot apical meristem function during Arabidopsis development

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Gene expression

pattern of pSTM::uidA activity ... in 7-day-old emb1611-2 seedlings ... a few emb1611-2 individuals showed light GUS staining in the center of the SAM

Leasure CD, Fiume E, Fletcher JC - The essential gene EMB1611 maintains shoot apical meristem function during Arabidopsis development

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Gene expression

In 17-day-old emb1611-2 plants, pSTM::uidA activity was invariably detected throughout the meristem

Leasure CD, Fiume E, Fletcher JC - The essential gene EMB1611 maintains shoot apical meristem function during Arabidopsis development

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Gene expression

EMB1611 ... is transcribed at low but equivalent levels in all tissues ... based on RT-PCR

Leasure CD, Fiume E, Fletcher JC - The essential gene EMB1611 maintains shoot apical meristem function during Arabidopsis development

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Gene expression

using in situ hybridization to examine the expression of WUS ... Among 14-day-old emb1611-2 seedlings, 50% showed WUS expression ... at lower levels than in wild-type seedlings ... in the SAM

Leasure CD, Fiume E, Fletcher JC - The essential gene EMB1611 maintains shoot apical meristem function during Arabidopsis development

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Gene expression

However, the expression of TSB1 was not affected in the mutant

Jing Y, Cui D, Bao F, Hu Z, Qin Z, Hu Y - Tryptophan deficiency affects organ growth by retarding cell expansion in Arabidopsis

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Gene expression

To investigate the function of FZR genes in plant development, we obtained two T-DNA insertion mutants for FZR2 from the SALK collection. The fzr2-1 (SALK_083656 ... lines carry insertions in the fourth exon ... Insertions were confirmed by sequencing. RT-PCR analysis showed that both alleles of fzr2 fail to produce full-length transcript

Larson-Rabin Z, Li Z, Masson PH, Day CD - FZR2/CCS52A1 expression is a determinant of endoreduplication and cell expansion in Arabidopsis

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Gene expression

leaf development by quantitative real-time RT-PCR ... steady expression ... FZR2 ... at 2 to 5 d

Larson-Rabin Z, Li Z, Masson PH, Day CD - FZR2/CCS52A1 expression is a determinant of endoreduplication and cell expansion in Arabidopsis

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Gene expression

leaf development by quantitative real-time RT-PCR ... steady expression of FZR1 ... at 2 to 5 d

Larson-Rabin Z, Li Z, Masson PH, Day CD - FZR2/CCS52A1 expression is a determinant of endoreduplication and cell expansion in Arabidopsis

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Gene expression

FZR2 expression in seedlings

Larson-Rabin Z, Li Z, Masson PH, Day CD - FZR2/CCS52A1 expression is a determinant of endoreduplication and cell expansion in Arabidopsis

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Gene expression

FZR2 expression in ... leaves

Larson-Rabin Z, Li Z, Masson PH, Day CD - FZR2/CCS52A1 expression is a determinant of endoreduplication and cell expansion in Arabidopsis

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Gene expression

To investigate the function of FZR genes in plant development, we obtained two T-DNA insertion mutants for FZR2 from the SALK collection ... fzr2-2 (SALK_101689) lines carry insertions in ... the second intron ... Insertions were confirmed by sequencing. RT-PCR analysis showed that both alleles of fzr2 fail to produce full-length transcript

Larson-Rabin Z, Li Z, Masson PH, Day CD - FZR2/CCS52A1 expression is a determinant of endoreduplication and cell expansion in Arabidopsis

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Gene expression

leaf development by quantitative real-time RT-PCR ... FZR3 was highly expressed in 3- and 5-d-old leaves

Larson-Rabin Z, Li Z, Masson PH, Day CD - FZR2/CCS52A1 expression is a determinant of endoreduplication and cell expansion in Arabidopsis

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Gene expression

The mutant pldε-1 ... resulted in loss of PLDε expression, as indicated by the absence of detectable PLDε transcript

Hong Y, Devaiah SP, Bahn SC, Thamasandra BN, Li M, Welti R, Wang X - Phospholipase D epsilon and phosphatidic acid enhance Arabidopsis nitrogen signaling and growth

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Gene expression

PLDε mRNA level was ... low in leaves

Hong Y, Devaiah SP, Bahn SC, Thamasandra BN, Li M, Welti R, Wang X - Phospholipase D epsilon and phosphatidic acid enhance Arabidopsis nitrogen signaling and growth

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Gene expression

in the AtMRB1 overexpressor plants ... the transcription level of ANT ... significantly upregulated

Guan H, Kang D, Fan M, Chen Z, Qu LJ - Overexpression of a new putative membrane protein gene AtMRB1 results in organ size enlargement in Arabidopsis

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Gene expression

in the AtMRB1 overexpressor plants ... the transcription level of ... ROT3 ... significantly upregulated

Guan H, Kang D, Fan M, Chen Z, Qu LJ - Overexpression of a new putative membrane protein gene AtMRB1 results in organ size enlargement in Arabidopsis

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Gene expression

To analyze the expression pattern of AtMRB1, we amplified a 1.8 kb long fragment from the AtMRB1 promoter and fused it to β-glucuronidase (GUS) prior to transformation into wild type Arabidopsis. Analysis of the transgenic plants showed that the expression of AtMRB1 was mainly focused in young leaves, hypocotyl, and in the roots of the seedlings

Guan H, Kang D, Fan M, Chen Z, Qu LJ - Overexpression of a new putative membrane protein gene AtMRB1 results in organ size enlargement in Arabidopsis

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Gene expression

in the AtMRB1 overexpressor plants ... the transcription level of ... BB ... not much altered

Guan H, Kang D, Fan M, Chen Z, Qu LJ - Overexpression of a new putative membrane protein gene AtMRB1 results in organ size enlargement in Arabidopsis

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Gene expression

In adult plants, AtMRB1 transcripts were mainly concentrated in the roots and flower organs, including young upper stems, shoot tips and shoot apical meristems (SAM), and flower buds (Figure 3D–M

Guan H, Kang D, Fan M, Chen Z, Qu LJ - Overexpression of a new putative membrane protein gene AtMRB1 results in organ size enlargement in Arabidopsis

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Gene expression

We transformed wild type Arabidopsis thaliana with the AtMRB1-green fluorescent protein (GFP) fusion protein construct driven by a CaMV 35S promoter and successfully obtained 35 independent transgenic lines used to study the subcellular localization using Confocal Imaging. As shown in Figure 2, green fluorescence was found in the membrane of the cells in undifferentiated cells in the root tip extension zone of AtMRB1-GFP transgenic plants

Guan H, Kang D, Fan M, Chen Z, Qu LJ - Overexpression of a new putative membrane protein gene AtMRB1 results in organ size enlargement in Arabidopsis

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Gene expression

in the AtMRB1 overexpressor plants ... the transcription level of ... AtGIF ... not much altered

Guan H, Kang D, Fan M, Chen Z, Qu LJ - Overexpression of a new putative membrane protein gene AtMRB1 results in organ size enlargement in Arabidopsis

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Gene expression

in the AtMRB1 overexpressor plants ... the transcription level of ... GRF5 ... significantly upregulated

Guan H, Kang D, Fan M, Chen Z, Qu LJ - Overexpression of a new putative membrane protein gene AtMRB1 results in organ size enlargement in Arabidopsis

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Gene expression

In adult plants, AtMRB1 transcripts were mainly concentrated in the roots and flower organs, including young upper stems, shoot tips and shoot apical meristems (SAM), and flower buds (Figure 3D–M

Guan H, Kang D, Fan M, Chen Z, Qu LJ - Overexpression of a new putative membrane protein gene AtMRB1 results in organ size enlargement in Arabidopsis

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Gene expression

Strong EXO expression was observed in rosette leaves

Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves

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Gene expression

proteomics approaches also identified the EXO ... as part of the cell wall proteome

Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves

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Gene expression

The EXO:HA protein was detected in the cell wall

Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves

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Gene expression

expression was observed in rosette leaves ... EXL5

Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves

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Gene expression

Senescing leaves ... displayed little EXO expression

Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves

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Gene expression

Real-time RT-PCR analysis demonstrated weaker EXP5 ... expression in the exo mutant in comparison to Col-0 wild-type plants

Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves

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Gene expression

Real-time RT-PCR analysis demonstrated weaker ... KCS1 expression in the exo mutant in comparison to Col-0 wild-type plants

Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves

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Gene expression

expression was observed in ... cotyledons ... EXL1

Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves

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Gene expression

proteomics approaches also identified ... EXL2 ... as part of the cell wall proteome

Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves

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Gene expression

expression was observed in rosette leaves ... EXL1

Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves

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Gene expression

expression was observed in ... cotyledons ... EXL3

Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves

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Gene expression

proteomics approaches also identified ... EXL3 ... as part of the cell wall proteome

Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves

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Gene expression

proteomics approaches also identified ... EXL4 ... as part of the cell wall proteome

Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves

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Gene expression

proteomics approaches also identified ... EXL1 ... as part of the cell wall proteome

Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves

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Gene expression

Strong EXO expression was observed in ... cotyledons

Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves

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Gene expression

EXL1 ... expression was also induced by BR

Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves

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Gene expression

expression was observed in ... cotyledons ... EXL5

Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves

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Gene expression

expression was observed in rosette leaves ... EXL3

Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves

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Gene expression

EXL5 expression was also induced by BR

Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves

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Gene expression

exo plants ... WAK1 (Wall-Associated Kinase 1) [22] transcript levels were reduced approximately 2.5-fold in the mutant

Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves

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Gene expression

a 35S::EXO:GFP construct was stably introduced into Arabidopsis wild-type plants. Green fluorescence was detected in the apoplast. Plasmolysed cells showed fluorescence in the apoplast as well

Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves

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Gene expression

EXL3 ... expression was also induced by BR

Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves

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Gene expression

CDKA;1 ... 2690-bp upstream region ... fused it to the β-glucuronidase (GUS) gene ... We then dissected the CDKA;1 promoter and observed GUS expression in tissues. Since Imajuku et al. (2001) have reported that the promoter region from − 1268 bp to the initiation codon was enough to reproduce the expression pattern driven by longer promoter fragments, we generated a deletion series of the 1290-bp region as shown in Fig. 2. The constructs, which were termed 7505, 7506, 7507, 7508, 7509, and 7510, differed with regard to the length of the 5′ region at 100-bp intervals. Seven-day-old seedlings harboring each reporter gene were subjected to GUS staining. We could not find any significant difference in the level and tissue specificity of the expressions of this gene among transgenic lines transformed with the constructs of 7501, 7505, 7506, 7507, 7508, and 7509 (Fig. 3). A shorter incubation in a GUS-staining buffer at a lower temperature (20 °C, 10 min) also showed no significant difference among these lines (Supplemental Fig. 1). However, GUS expression in the 7510 seedlings was lower in both the shoots and roots as compared to that in the above-mentioned lines, although no obvious change was observed in the expression pattern (Fig. 3). We could observe similar quantitative differences in the embryos and inflorescences (Supplemental Fig. 2). These results suggest that the region from − 890 bp to − 791 bp contains cis-regulatory element(s) that promote CDKA;1 expression independently of tissues ... Therefore, we constructed GUS reporter genes with 3′-end truncations of the 1290-bp region. The 7505, 7502R, 7503R, 7504R, 7505R, 7506R, and 7507R constructs differed with regard to the length of the 3′ region at 100-bp intervals (Fig. 2). In the 7-day-old seedlings, we found a significant decrease in the level of GUS expression between the 7502R and 7503R constructs (Fig. 4A). A similar reduction was also noted in embryos (Supplemental Fig. 3), indicating the presence of another cis-regulatory element between − 200 and − 101 bp. In the 7503R–7505R lines, the expression in the roots was gradually reduced as the 3′ end became shorter and, in the roots of the 7506R or 7507R plants, we could not detect any GUS expression (Fig. 4B). Despite the disappearance of GUS expression in roots, we could observe GUS signals in young leaves of the 7506R and 7507R lines (Fig. 4B), indicating the presence of root-specific regulatory elements that promote CDKA;1 expression. Presence of a promoter region enhancing CDKA;1 expression in the leaf epidermis ... 7509F/7R, which carries the region between − 890 bp and − 601 bp (Fig. 2). Although we included the region from − 890 bp to − 791 bp that promotes CDKA;1 expression as mentioned above, the GUS signal was very weak as compared to that in 7507R. However, we could again identify epidermis-specific expression (Fig. 5D), suggesting that this region contains enough information to promote epidermal expression in leaves ... then introduced the 7507R reporter gene into the phbulosa-1d (phb-1d) mutants ... Although the stipules were stained in a similar manner to the wild-type background, the GUS expression in the leaf epidermis was severely suppressed, and almost no expression was detected in any cell type

Adachi S, Nobusawa T, Umeda M - Quantitative and cell type-specific transcriptional regulation of A-type cyclin-dependent kinase in Arabidopsis thaliana

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Gene expression

CDKA;1 ... 2690-bp upstream region ... fused it to the β-glucuronidase (GUS) gene ... weakly stained mesophyll cells in the leaves ... We then dissected the CDKA;1 promoter and observed GUS expression in tissues. Since Imajuku et al. (2001) have reported that the promoter region from − 1268 bp to the initiation codon was enough to reproduce the expression pattern driven by longer promoter fragments, we generated a deletion series of the 1290-bp region as shown in Fig. 2. The constructs, which were termed 7505, 7506, 7507, 7508, 7509, and 7510, differed with regard to the length of the 5′ region at 100-bp intervals. Seven-day-old seedlings harboring each reporter gene were subjected to GUS staining. We could not find any significant difference in the level and tissue specificity of the expressions of this gene among transgenic lines transformed with the constructs of 7501, 7505, 7506, 7507, 7508, and 7509 (Fig. 3). A shorter incubation in a GUS-staining buffer at a lower temperature (20 °C, 10 min) also showed no significant difference among these lines (Supplemental Fig. 1). However, GUS expression in the 7510 seedlings was lower in both the shoots and roots as compared to that in the above-mentioned lines, although no obvious change was observed in the expression pattern (Fig. 3). We could observe similar quantitative differences in the embryos and inflorescences (Supplemental Fig. 2). These results suggest that the region from − 890 bp to − 791 bp contains cis-regulatory element(s) that promote CDKA;1 expression independently of tissues ... Therefore, we constructed GUS reporter genes with 3′-end truncations of the 1290-bp region. The 7505, 7502R, 7503R, 7504R, 7505R, 7506R, and 7507R constructs differed with regard to the length of the 3′ region at 100-bp intervals (Fig. 2). In the 7-day-old seedlings, we found a significant decrease in the level of GUS expression between the 7502R and 7503R constructs (Fig. 4A). A similar reduction was also noted in embryos (Supplemental Fig. 3), indicating the presence of another cis-regulatory element between − 200 and − 101 bp. In the 7503R–7505R lines, the expression in the roots was gradually reduced as the 3′ end became shorter and, in the roots of the 7506R or 7507R plants, we could not detect any GUS expression (Fig. 4B). Despite the disappearance of GUS expression in roots, we could observe GUS signals in young leaves of the 7506R and 7507R lines (Fig. 4B), indicating the presence of root-specific regulatory elements that promote CDKA;1 expression. Presence of a promoter region enhancing CDKA;1 expression in the leaf epidermis ... we created another reporter construct, 7509F/7R, which carries the region between − 890 bp and − 601 bp ... we could again identify epidermis-specific expression (Fig. 5D), suggesting that this region contains enough information to promote epidermal expression in leaves

Adachi S, Nobusawa T, Umeda M - Quantitative and cell type-specific transcriptional regulation of A-type cyclin-dependent kinase in Arabidopsis thaliana

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Gene expression

We then dissected the CDKA;1 promoter and observed GUS expression in tissues ... we constructed GUS reporter genes with 3′-end truncations of the 1290-bp region. The 7505, 7502R, 7503R, 7504R, 7505R, 7506R, and 7507R constructs differed with regard to the length of the 3′ region at 100-bp intervals ... We then conducted a detailed analysis of the epidermal expression of this gene by using the 7507R reporter gene. Since the longitudinal sections of shoot apices showed biased GUS staining on the abaxial side of the leaf epidermis (Fig. 5C), we also created transverse sections of the SAM and leaves. As expected, GUS expression was higher on the abaxial side of young leaves, and only a trace level of expression was observed on the adaxial side

Adachi S, Nobusawa T, Umeda M - Quantitative and cell type-specific transcriptional regulation of A-type cyclin-dependent kinase in Arabidopsis thaliana

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Gene expression

We then dissected the CDKA;1 promoter and observed GUS expression in tissues ... To examine cell type-specific regulation of CDKA;1 expression, we made sections of GUS-stained shoot apices ... GUS expression was observed in the ... leaf primordia

Adachi S, Nobusawa T, Umeda M - Quantitative and cell type-specific transcriptional regulation of A-type cyclin-dependent kinase in Arabidopsis thaliana

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Gene expression

We then dissected the CDKA;1 promoter and observed GUS expression in tissues ... we created another reporter construct, 7509F/7R, which carries the region between − 890 bp and − 601 bp (Fig. 2). Although we included the region from − 890 bp to − 791 bp that promotes CDKA;1 expression as mentioned above, the GUS signal was very weak as compared to that in 7507R. However, we could again identify epidermis-specific expression (Fig. 5D), suggesting that this region contains enough information to promote epidermal expression in leaves

Adachi S, Nobusawa T, Umeda M - Quantitative and cell type-specific transcriptional regulation of A-type cyclin-dependent kinase in Arabidopsis thaliana

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Gene expression

We then dissected the CDKA;1 promoter and observed GUS expression in tissues ... To examine cell type-specific regulation of CDKA;1 expression, we made sections of GUS-stained shoot apices ... GUS expression was observed in the ... developing leaves

Adachi S, Nobusawa T, Umeda M - Quantitative and cell type-specific transcriptional regulation of A-type cyclin-dependent kinase in Arabidopsis thaliana

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Gene expression

CDKA ... 1 ... 2690-bp upstream region ... fused it to the β-glucuronidase (GUS) gene ... an intense signal in the stomata ... in the leaves ... We then dissected the CDKA;1 promoter and observed GUS expression in tissues. Since Imajuku et al. (2001) have reported that the promoter region from − 1268 bp to the initiation codon was enough to reproduce the expression pattern driven by longer promoter fragments, we generated a deletion series of the 1290-bp region as shown in Fig. 2. The constructs, which were termed 7505, 7506, 7507, 7508, 7509, and 7510, differed with regard to the length of the 5′ region at 100-bp intervals. Seven-day-old seedlings harboring each reporter gene were subjected to GUS staining. We could not find any significant difference in the level and tissue specificity of the expressions of this gene among transgenic lines transformed with the constructs of 7501, 7505, 7506, 7507, 7508, and 7509 (Fig. 3). A shorter incubation in a GUS-staining buffer at a lower temperature (20 °C, 10 min) also showed no significant difference among these lines (Supplemental Fig. 1). However, GUS expression in the 7510 seedlings was lower in both the shoots and roots as compared to that in the above-mentioned lines, although no obvious change was observed in the expression pattern (Fig. 3). We could observe similar quantitative differences in the embryos and inflorescences (Supplemental Fig. 2). These results suggest that the region from − 890 bp to − 791 bp contains cis-regulatory element(s) that promote CDKA;1 expression independently of tissues ... Therefore, we constructed GUS reporter genes with 3′-end truncations of the 1290-bp region. The 7505, 7502R, 7503R, 7504R, 7505R, 7506R, and 7507R constructs differed with regard to the length of the 3′ region at 100-bp intervals (Fig. 2). In the 7-day-old seedlings, we found a significant decrease in the level of GUS expression between the 7502R and 7503R constructs (Fig. 4A). A similar reduction was also noted in embryos (Supplemental Fig. 3), indicating the presence of another cis-regulatory element between − 200 and − 101 bp. In the 7503R–7505R lines, the expression in the roots was gradually reduced as the 3′ end became shorter and, in the roots of the 7506R or 7507R plants, we could not detect any GUS expression (Fig. 4B). Despite the disappearance of GUS expression in roots, we could observe GUS signals in young leaves of the 7506R and 7507R lines (Fig. 4B), indicating the presence of root-specific regulatory elements that promote CDKA;1 expression. Presence of a promoter region enhancing CDKA;1 expression in the leaf epidermis ... we created another reporter construct, 7509F/7R, which carries the region between − 890 bp and − 601 bp ... we could again identify epidermis-specific expression (Fig. 5D), suggesting that this region contains enough information to promote epidermal expression in leaves

Adachi S, Nobusawa T, Umeda M - Quantitative and cell type-specific transcriptional regulation of A-type cyclin-dependent kinase in Arabidopsis thaliana

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Gene expression

We then dissected the CDKA;1 promoter and observed GUS expression in tissues ... 7503R exhibited lower GUS expression in the SAM and no expression in the inner layers of leaves. The results showed that the epidermis of leaves and trichomes were prominently GUS-stained (Fig. 5C). This indicates that the region between − 200 bp and − 101 bp is associated with CDKA;1 expression in the ... leaves except for the epidermis ... The epidermis-specific expression pattern was also observed in 7504R–7507R ... In situ hybridization using probes for transcripts of GUS showed the similar epidermis-specific expression in leaves of the 7503R and 7507R lines

Adachi S, Nobusawa T, Umeda M - Quantitative and cell type-specific transcriptional regulation of A-type cyclin-dependent kinase in Arabidopsis thaliana

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Gene expression

We then dissected the CDKA;1 promoter and observed GUS expression in tissues ... we constructed GUS reporter genes with 3′-end truncations of the 1290-bp region. The 7505, 7502R, 7503R, 7504R, 7505R, 7506R, and 7507R constructs differed with regard to the length of the 3′ region at 100-bp intervals ... 7503R exhibited lower GUS expression in the SAM and no expression in the inner layers of leaves. The results showed that the epidermis of leaves and trichomes were prominently GUS-stained (Fig. 5C). This indicates that the region between − 200 bp and − 101 bp is associated with CDKA;1 expression in the ... leaves except for the epidermis ... The epidermis-specific expression pattern was also observed in 7504R–7507R

Adachi S, Nobusawa T, Umeda M - Quantitative and cell type-specific transcriptional regulation of A-type cyclin-dependent kinase in Arabidopsis thaliana

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Gene expression

We then dissected the CDKA;1 promoter and observed GUS expression in tissues ... we constructed GUS reporter genes with 3′-end truncations of the 1290-bp region. The 7505, 7502R, 7503R, 7504R, 7505R, 7506R, and 7507R constructs differed with regard to the length of the 3′ region at 100-bp intervals ... We then conducted a detailed analysis of the epidermal expression of this gene by using the 7507R reporter gene. Since the longitudinal sections of shoot apices showed biased GUS staining on the abaxial side of the leaf epidermis (Fig. 5C), we also created transverse sections of the SAM and leaves. As expected, GUS expression was higher on the abaxial side of young leaves, and only a trace level of expression was observed on the adaxial side

Adachi S, Nobusawa T, Umeda M - Quantitative and cell type-specific transcriptional regulation of A-type cyclin-dependent kinase in Arabidopsis thaliana

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Gene expression

We then dissected the CDKA;1 promoter and observed GUS expression in tissues ... 7503R exhibited lower GUS expression in the SAM and no expression in the inner layers of leaves. The results showed that the epidermis of leaves and trichomes were prominently GUS-stained (Fig. 5C). This indicates that the region between − 200 bp and − 101 bp is associated with CDKA;1 expression in the ... leaves except for the epidermis

Adachi S, Nobusawa T, Umeda M - Quantitative and cell type-specific transcriptional regulation of A-type cyclin-dependent kinase in Arabidopsis thaliana

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Gene expression

MED21 ... Significant expression in rosette leaves

Dhawan R, Luo H, Foerster AM, Abuqamar S, Du HN, Briggs SD, Mittelsten Scheid O, Mengiste T - HISTONE MONOUBIQUITINATION1 interacts with a subunit of the mediator complex and regulates defense against necrotrophic fungal pathogens in Arabidopsis

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Gene expression

HERK1 ... expression levels ... were ... increased in the constitutive BR-response mutant bes1-D

Guo H, Li L, Ye H, Yu X, Algreen A, Yin Y - Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana

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Gene expression

THE1 ... localized to the plasma membrane

Guo H, Li L, Ye H, Yu X, Algreen A, Yin Y - Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana

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Gene expression

FER ... universally expressed in most vegetative tissues, including leaves, stems and roots as revealed by our promoter-GUS (β-glucuronidase) reporter gene studies

Guo H, Li L, Ye H, Yu X, Algreen A, Yin Y - Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana

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Gene expression

HERK1 ... expression is particularly strong in the regions undergoing cell elongation, such as in hypocotyls and leaf petioles

Guo H, Li L, Ye H, Yu X, Algreen A, Yin Y - Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana

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Gene expression

In our effort to identify and characterize BR regulated genes, we found that HERK1, THE1 and FER are induced by BRs as revealed by both published (15) and publicly available microarray data (http://bbc.botany.utoronto.ca/efp/cgi-bin/efpWeb.cgi). HERK1, THE1, and FER are the most highly induced genes by BR and, therefore, were chosen for further study. Our quantitative RT-PCR experiments confirmed that brassinolide (BL), the most active BR, up-regulated these RLKs by ≈20–80% in seedlings and/or adult plants (Fig. 1A

Guo H, Li L, Ye H, Yu X, Algreen A, Yin Y - Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana

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Gene expression

In our effort to identify and characterize BR regulated genes, we found that HERK1, THE1 and FER are induced by BRs as revealed by both published (15) and publicly available microarray data (http://bbc.botany.utoronto.ca/efp/cgi-bin/efpWeb.cgi). HERK1, THE1, and FER are the most highly induced genes by BR and, therefore, were chosen for further study. Our quantitative RT-PCR experiments confirmed that brassinolide (BL), the most active BR, up-regulated these RLKs by ≈20–80% in seedlings and/or adult plants (Fig. 1A

Guo H, Li L, Ye H, Yu X, Algreen A, Yin Y - Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana

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Gene expression

FER ... localized to the plasma membrane

Guo H, Li L, Ye H, Yu X, Algreen A, Yin Y - Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana

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Gene expression

FER ... expression levels ... were reduced in the loss-of-function mutant bri1

Guo H, Li L, Ye H, Yu X, Algreen A, Yin Y - Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana

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Gene expression

FER ... expression is particularly strong in the regions undergoing cell elongation, such as in hypocotyls and leaf petioles

Guo H, Li L, Ye H, Yu X, Algreen A, Yin Y - Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana

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Gene expression

HERK1-GFP fusion localized to the plasma membrane when expressed in Arabidopsis protoplasts and plants

Guo H, Li L, Ye H, Yu X, Algreen A, Yin Y - Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana

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Gene expression

THE1 ... universally expressed in most vegetative tissues, including leaves, stems and roots as revealed by our promoter-GUS (β-glucuronidase) reporter gene studies

Guo H, Li L, Ye H, Yu X, Algreen A, Yin Y - Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana

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Gene expression

HERK1 ... expression levels ... were reduced in the loss-of-function mutant bri1

Guo H, Li L, Ye H, Yu X, Algreen A, Yin Y - Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana

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Gene expression

HERK1 ... universally expressed in most vegetative tissues, including leaves, stems and roots as revealed by our promoter-GUS (β-glucuronidase) reporter gene studies

Guo H, Li L, Ye H, Yu X, Algreen A, Yin Y - Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana

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Gene expression

THE1 ... expression levels ... were ... increased in the constitutive BR-response mutant bes1-D

Guo H, Li L, Ye H, Yu X, Algreen A, Yin Y - Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana

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Gene expression

THE1 ... expression levels ... were reduced in the loss-of-function mutant bri1

Guo H, Li L, Ye H, Yu X, Algreen A, Yin Y - Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana

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Gene expression

In our effort to identify and characterize BR regulated genes, we found that HERK1, THE1 and FER are induced by BRs as revealed by both published (15) and publicly available microarray data (http://bbc.botany.utoronto.ca/efp/cgi-bin/efpWeb.cgi). HERK1, THE1, and FER are the most highly induced genes by BR and, therefore, were chosen for further study. Our quantitative RT-PCR experiments confirmed that brassinolide (BL), the most active BR, up-regulated these RLKs by ≈20–80% in seedlings and/or adult plants (Fig. 1A

Guo H, Li L, Ye H, Yu X, Algreen A, Yin Y - Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana

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Gene expression

FER ... expression levels ... were ... increased in the constitutive BR-response mutant bes1-D

Guo H, Li L, Ye H, Yu X, Algreen A, Yin Y - Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana

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Gene expression

THE1 ... expression is particularly strong in the regions undergoing cell elongation, such as in hypocotyls and leaf petioles

Guo H, Li L, Ye H, Yu X, Algreen A, Yin Y - Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana

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Gene expression

The expression pattern of SPI was determined by RT-PCR analysis. SPI could be amplified from total RNA of all organs tested, including the flowers, cauline leaves, stem, rosette leaves, hypocotyl and roots

Saedler R, Jakoby M, Marin B, Galiana-Jaime E, Hülskamp M - The cell morphogenesis gene SPIRRIG in Arabidopsis encodes a WD/BEACH domain protein

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Gene expression

When we examined the accumula- tion of AN3 transcript in the ... oli5 ... by RT-PCR analysis, it accumulated at a comparable level to that in wild-type

Fujikura U, Horiguchi G, Ponce MR, Micol JL, Tsukaya H - Coordination of cell proliferation and cell expansion mediated by ribosome-related processes in the leaves of Arabidopsis thaliana

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Gene expression

When we examined the accumula- tion of AN3 transcript in the ... oli7 ... by RT-PCR analysis, it accumulated at a comparable level to that in wild-type

Fujikura U, Horiguchi G, Ponce MR, Micol JL, Tsukaya H - Coordination of cell proliferation and cell expansion mediated by ribosome-related processes in the leaves of Arabidopsis thaliana

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Gene expression

When we examined the accumula- tion of AN3 transcript in the oli2 ... by RT-PCR analysis, it accumulated at a comparable level to that in wild-type

Fujikura U, Horiguchi G, Ponce MR, Micol JL, Tsukaya H - Coordination of cell proliferation and cell expansion mediated by ribosome-related processes in the leaves of Arabidopsis thaliana

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Gene expression

The bil1bil2 mutation increases the SAUR-ACS1 transcript level by approximately 2-fold

Yan Z, Zhao J, Peng P, Chihara RK, Li J - BIN2 functions redundantly with other Arabidopsis GSK3-like kinases to regulate brassinosteroid signaling

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Gene expression

without BR treatment, bin2-3 mutants accumulated more SAUR-AC1 transcripts ... than wild-type plants

Yan Z, Zhao J, Peng P, Chihara RK, Li J - BIN2 functions redundantly with other Arabidopsis GSK3-like kinases to regulate brassinosteroid signaling

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Gene expression

without BR treatment, bin2-3 mutants accumulate an easily detectable amount of hypophosphorylated BES1 compared with wild-type control plants grown under the same growth conditions

Yan Z, Zhao J, Peng P, Chihara RK, Li J - BIN2 functions redundantly with other Arabidopsis GSK3-like kinases to regulate brassinosteroid signaling

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Gene expression

1 h of BL treatment stimulated the expression of SAUR-AC1

Yan Z, Zhao J, Peng P, Chihara RK, Li J - BIN2 functions redundantly with other Arabidopsis GSK3-like kinases to regulate brassinosteroid signaling

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Gene expression

1 h of BL treatment ... inhibited the accumulation of transcripts of ... CPD

Yan Z, Zhao J, Peng P, Chihara RK, Li J - BIN2 functions redundantly with other Arabidopsis GSK3-like kinases to regulate brassinosteroid signaling

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Gene expression

1 h of BL treatment ... inhibited the accumulation of transcripts of ... DWF4

Yan Z, Zhao J, Peng P, Chihara RK, Li J - BIN2 functions redundantly with other Arabidopsis GSK3-like kinases to regulate brassinosteroid signaling

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Gene expression

SAUR-AC1 expression was significantly enhanced in the bin2-3bil1bil3 triple mutant, with a nearly 7-fold increase compared with an approximately 3-fold BR-elicited induction in wild-type seedlings

Yan Z, Zhao J, Peng P, Chihara RK, Li J - BIN2 functions redundantly with other Arabidopsis GSK3-like kinases to regulate brassinosteroid signaling

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Gene expression

without BR treatment, bin2-3 mutants accumulated ... lower levels of DWF4 ... mRNAs than wild-type plants

Yan Z, Zhao J, Peng P, Chihara RK, Li J - BIN2 functions redundantly with other Arabidopsis GSK3-like kinases to regulate brassinosteroid signaling

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Gene expression

without BR treatment, bin2-3 mutants accumulated ... lower levels of ... CPD mRNAs than wild-type plants

Yan Z, Zhao J, Peng P, Chihara RK, Li J - BIN2 functions redundantly with other Arabidopsis GSK3-like kinases to regulate brassinosteroid signaling

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Gene expression

bin2-3bil1bil2 ... the transcript level of CPD was reduced by approximately 80% in the triple mutant compared with an approximately 40% reduction in BR-treated wild-type plants

Yan Z, Zhao J, Peng P, Chihara RK, Li J - BIN2 functions redundantly with other Arabidopsis GSK3-like kinases to regulate brassinosteroid signaling

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Gene expression

wild-type plants predominantly accumulate the hyperphosphorylated form of BES1, and BL treatment results in rapid disappearance of the hyperphosphorylated BES1 accompanied by accumulation of hypophosphorylated BES1, which has a faster electrophoretic mobility on western blot

Yan Z, Zhao J, Peng P, Chihara RK, Li J - BIN2 functions redundantly with other Arabidopsis GSK3-like kinases to regulate brassinosteroid signaling

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Gene expression

The bil1bil2 mutation ... reduces the CPD mRNA level by approximately 30%

Yan Z, Zhao J, Peng P, Chihara RK, Li J - BIN2 functions redundantly with other Arabidopsis GSK3-like kinases to regulate brassinosteroid signaling

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Gene expression

pEPF2:GUS expressed ... in very young leaves and during leaf development became restricted to meristemoids and GMCs

Hunt L, Gray JE - The signaling peptide EPF2 controls asymmetric cell divisions during stomatal development

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Gene expression

In transgenic plants harboring EPF1–GFP ... EPF1 ... activated green fluorescent protein ... in stomatal precursors

Hara K, Yokoo T, Kajita R, Onishi T, Yahata S, Peterson KM, Torii KU, Kakimoto T - Epidermal cell density is autoregulated via a secretory peptide, EPIDERMAL PATTERNING FACTOR 2 in Arabidopsis leaves

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Gene expression

An in situ RNA hybridization experiment showed that the EPF2 message ... was also expressed in another type of cells with a small quadrangular shape, which are perhaps cells with asymmetric cell division competency, including MMCs

Hara K, Yokoo T, Kajita R, Onishi T, Yahata S, Peterson KM, Torii KU, Kakimoto T - Epidermal cell density is autoregulated via a secretory peptide, EPIDERMAL PATTERNING FACTOR 2 in Arabidopsis leaves

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Gene expression

Overexpression of ... EPF2 decreased the number of cells expressing EPF1–GFP

Hara K, Yokoo T, Kajita R, Onishi T, Yahata S, Peterson KM, Torii KU, Kakimoto T - Epidermal cell density is autoregulated via a secretory peptide, EPIDERMAL PATTERNING FACTOR 2 in Arabidopsis leaves

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Gene expression

EPF2–GFP was not detected in guard cells nor in any non-epidermal cells

Hara K, Yokoo T, Kajita R, Onishi T, Yahata S, Peterson KM, Torii KU, Kakimoto T - Epidermal cell density is autoregulated via a secretory peptide, EPIDERMAL PATTERNING FACTOR 2 in Arabidopsis leaves

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Gene expression

In ... transgenic plants harboring ... EPF2–GFP ... At1g34245 ... activated green fluorescent protein ... in stomatal precursors

Hara K, Yokoo T, Kajita R, Onishi T, Yahata S, Peterson KM, Torii KU, Kakimoto T - Epidermal cell density is autoregulated via a secretory peptide, EPIDERMAL PATTERNING FACTOR 2 in Arabidopsis leaves

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Gene expression

EPF1–GFP ... were expressed ... not in the spch homozygotes

Hara K, Yokoo T, Kajita R, Onishi T, Yahata S, Peterson KM, Torii KU, Kakimoto T - Epidermal cell density is autoregulated via a secretory peptide, EPIDERMAL PATTERNING FACTOR 2 in Arabidopsis leaves

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Gene expression

Overexpression of neither EPF1 ... affected TMM–GFP expression

Hara K, Yokoo T, Kajita R, Onishi T, Yahata S, Peterson KM, Torii KU, Kakimoto T - Epidermal cell density is autoregulated via a secretory peptide, EPIDERMAL PATTERNING FACTOR 2 in Arabidopsis leaves

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Gene expression

Reverse transcription–PCR (RT–PCR) analysis revealed that EPF2 ... is preferentially expressed in the aerial organs

Hara K, Yokoo T, Kajita R, Onishi T, Yahata S, Peterson KM, Torii KU, Kakimoto T - Epidermal cell density is autoregulated via a secretory peptide, EPIDERMAL PATTERNING FACTOR 2 in Arabidopsis leaves

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Gene expression

Overexpression of neither ... EPF2 affected TMM–GFP expression

Hara K, Yokoo T, Kajita R, Onishi T, Yahata S, Peterson KM, Torii KU, Kakimoto T - Epidermal cell density is autoregulated via a secretory peptide, EPIDERMAL PATTERNING FACTOR 2 in Arabidopsis leaves

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Gene expression

Overexpression of ... EPF1 ... decreased the number of cells expressing EPF1–GFP

Hara K, Yokoo T, Kajita R, Onishi T, Yahata S, Peterson KM, Torii KU, Kakimoto T - Epidermal cell density is autoregulated via a secretory peptide, EPIDERMAL PATTERNING FACTOR 2 in Arabidopsis leaves

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Gene expression

An in situ RNA hybridization experiment showed that ... During early leaf development, the onset of the EPF2–GFP signal preceded the emergence of meristemoids ... signal was later detected in meristemoids, their sister cells and GMCs

Hara K, Yokoo T, Kajita R, Onishi T, Yahata S, Peterson KM, Torii KU, Kakimoto T - Epidermal cell density is autoregulated via a secretory peptide, EPIDERMAL PATTERNING FACTOR 2 in Arabidopsis leaves

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Gene expression

Reverse transcription–PCR (RT–PCR) analysis revealed that ... EPF1 ... is preferentially expressed in the aerial organs

Hara K, Yokoo T, Kajita R, Onishi T, Yahata S, Peterson KM, Torii KU, Kakimoto T - Epidermal cell density is autoregulated via a secretory peptide, EPIDERMAL PATTERNING FACTOR 2 in Arabidopsis leaves

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Gene expression

EPF2–GFP ... were expressed ... not in the spch homozygotes

Hara K, Yokoo T, Kajita R, Onishi T, Yahata S, Peterson KM, Torii KU, Kakimoto T - Epidermal cell density is autoregulated via a secretory peptide, EPIDERMAL PATTERNING FACTOR 2 in Arabidopsis leaves

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Gene expression

overexpression of EPF1 ... did not reduce the number of cells expressing EPF2–GFP

Hara K, Yokoo T, Kajita R, Onishi T, Yahata S, Peterson KM, Torii KU, Kakimoto T - Epidermal cell density is autoregulated via a secretory peptide, EPIDERMAL PATTERNING FACTOR 2 in Arabidopsis leaves

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Gene expression

EPF2-overexpressors had a decreased number of cells that express EPF2–GFP

Hara K, Yokoo T, Kajita R, Onishi T, Yahata S, Peterson KM, Torii KU, Kakimoto T - Epidermal cell density is autoregulated via a secretory peptide, EPIDERMAL PATTERNING FACTOR 2 in Arabidopsis leaves

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Gene expression

TMM–GFP ... were expressed ... not in the spch homozygotes

Hara K, Yokoo T, Kajita R, Onishi T, Yahata S, Peterson KM, Torii KU, Kakimoto T - Epidermal cell density is autoregulated via a secretory peptide, EPIDERMAL PATTERNING FACTOR 2 in Arabidopsis leaves

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Gene expression

in 35S:MIR396 transgenic plants ... levels of ... AtGRF3 transcripts were ... lower than those in control plants

Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis

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Gene expression

AtGRF8 ... mRNA levels were reduced in transgenic 35S:MIR396 plant leaves relative to those in control plants

Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis

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Gene expression

AtGRF4 mRNA levels were also reduced in transgenic 35S:MIR396 plant leaves compared with control plants

Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis

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Gene expression

AtGRF7 ... mRNA levels were reduced in transgenic 35S:MIR396 plant leaves relative to those in control plants

Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis

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Gene expression

Both RNA gel blot analysis and RT-PCR analysis showed that the expression levels of AN3 were significantly reduced in the transgenic 35S:MIR396a ... plants

Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis

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Gene expression

Wild-type plants (Col-0) (3 weeks old) grown in soil were subjected to drought stress by withholding watering and the levels of miR396 were determined by RNA blot analysis. As shown in Fig. 11, the level of miR396 was slightly increased in 1 day of watering withholding and was further elevated 2 days later. The levels of miR396 remained substantially higher after 4 and 5 days of drought stress than those detected prior to drought stress treatment. These results indicated that the level of miR396 was induced by drought stress

Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis

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Gene expression

AtGRF8 ... mRNA levels were reduced in transgenic 35S:MIR396 plant leaves relative to those in control plants

Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis

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Gene expression

in 35S:MIR396 transgenic plants ... levels of ... AtGRF3 transcripts were ... lower than those in control plants

Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis

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Gene expression

in 35S:MIR396 transgenic plants ... levels of AtGRF1 ... transcripts were ... lower than those in control plants

Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis

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Gene expression

in 35S:MIR396 transgenic plants ... levels of AtGRF1 ... transcripts were ... lower than those in control plants

Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis

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Gene expression

the transcript levels for ... AtGRF3 ... were significantly lower after 4 days of watering withholding than those detected at the beginning of drought stress

Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis

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Gene expression

AtGRF9 mRNA levels were reduced in transgenic 35S:MIR396 plant leaves relative to those in control plants

Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis

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Gene expression

the transcript levels for ... AN3 were significantly lower after 4 days of watering withholding than those detected at the beginning of drought stress

Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis

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Gene expression

in 35S:MIR396 transgenic plants ... levels of ... AtGRF2 ... transcripts were ... lower than those in control plants

Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis

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Gene expression

in 35S:MIR396 transgenic plants ... levels of ... AtGRF2 ... transcripts were ... lower than those in control plants

Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis

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Gene expression

AtGRF4 mRNA levels were also reduced in transgenic 35S:MIR396 plant leaves compared with control plants

Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis

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Gene expression

AtGRF9 mRNA levels were reduced in transgenic 35S:MIR396 plant leaves relative to those in control plants

Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis

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Gene expression

AtGRF7 ... mRNA levels were reduced in transgenic 35S:MIR396 plant leaves relative to those in control plants

Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis

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Gene expression

miR396 was broadly an expression profile and strongly expressed in 6-day-old seedlings and rosette leaves

Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis

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Gene expression

the transcript levels for AtGRF1 ... were significantly lower after 4 days of watering withholding than those detected at the beginning of drought stress

Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis

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Gene expression

Both RNA gel blot analysis and RT-PCR analysis showed that the expression levels of AN3 were significantly reduced in the transgenic ... 35S:MIR396b plants

Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis

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Gene expression

Transcripts for AtRPT2a ... were detectable in all organs tested at both 16 and 45 days after sowing (DAS)

Sonoda Y, Sako K, Maki Y, Yamazaki N, Yamamoto H, Ikeda A, Yamaguchi J - Regulation of leaf organ size by the Arabidopsis RPT2a 19S proteasome subunit

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Gene expression

At the proliferation stage (11 DAS), there was no difference in KRP2 transcript accumulation between the wild type and rpt2a-2

Sonoda Y, Sako K, Maki Y, Yamazaki N, Yamamoto H, Ikeda A, Yamaguchi J - Regulation of leaf organ size by the Arabidopsis RPT2a 19S proteasome subunit

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Gene expression

Transcripts for ... AtRPT2b were detectable in all organs tested at both 16 and 45 days after sowing (DAS)

Sonoda Y, Sako K, Maki Y, Yamazaki N, Yamamoto H, Ikeda A, Yamaguchi J - Regulation of leaf organ size by the Arabidopsis RPT2a 19S proteasome subunit

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Gene expression

AtRPT2a ... was detectable in ... trichomes

Sonoda Y, Sako K, Maki Y, Yamazaki N, Yamamoto H, Ikeda A, Yamaguchi J - Regulation of leaf organ size by the Arabidopsis RPT2a 19S proteasome subunit

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Gene expression

At the proliferation stage (11 DAS) ... the expression of ... CYCB1;1 ... was increased in rpt2a-2 compared with the wild type

Sonoda Y, Sako K, Maki Y, Yamazaki N, Yamamoto H, Ikeda A, Yamaguchi J - Regulation of leaf organ size by the Arabidopsis RPT2a 19S proteasome subunit

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Gene expression

The AtRPT2a promoter showed strong activity in the shoot meristem

Sonoda Y, Sako K, Maki Y, Yamazaki N, Yamamoto H, Ikeda A, Yamaguchi J - Regulation of leaf organ size by the Arabidopsis RPT2a 19S proteasome subunit

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Gene expression

AtRPT2a ... was detectable in various tissues, such as expanded cotyledons, vascular cells and trichomes

Sonoda Y, Sako K, Maki Y, Yamazaki N, Yamamoto H, Ikeda A, Yamaguchi J - Regulation of leaf organ size by the Arabidopsis RPT2a 19S proteasome subunit

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Gene expression

AtRPT2b ... was detectable in ... trichomes

Sonoda Y, Sako K, Maki Y, Yamazaki N, Yamamoto H, Ikeda A, Yamaguchi J - Regulation of leaf organ size by the Arabidopsis RPT2a 19S proteasome subunit

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Gene expression

AtRPT2b ... was detectable in various tissues, such as expanded cotyledons, vascular cells and trichomes

Sonoda Y, Sako K, Maki Y, Yamazaki N, Yamamoto H, Ikeda A, Yamaguchi J - Regulation of leaf organ size by the Arabidopsis RPT2a 19S proteasome subunit

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Gene expression

At the proliferation stage (11 DAS) ... the expression of ... KRP1 was increased in rpt2a-2 compared with the wild type

Sonoda Y, Sako K, Maki Y, Yamazaki N, Yamamoto H, Ikeda A, Yamaguchi J - Regulation of leaf organ size by the Arabidopsis RPT2a 19S proteasome subunit

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Gene expression

At the proliferation stage (11 DAS) ... the expression of CDKB1;1 ... was increased in rpt2a-2 compared with the wild type

Sonoda Y, Sako K, Maki Y, Yamazaki N, Yamamoto H, Ikeda A, Yamaguchi J - Regulation of leaf organ size by the Arabidopsis RPT2a 19S proteasome subunit

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Gene expression

AtRPT2b showed strong activity in both the shoot and root meristems

Sonoda Y, Sako K, Maki Y, Yamazaki N, Yamamoto H, Ikeda A, Yamaguchi J - Regulation of leaf organ size by the Arabidopsis RPT2a 19S proteasome subunit

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Gene expression

At4g37740/GRF2 ... was also increased in the gra-D mutants

Horiguchi G, Gonzalez N, Beemster GT, Inzé D, Tsukaya H - Impact of segmental chromosomal duplications on leaf size in the grandifolia-D mutants of Arabidopsis thaliana

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Gene expression

we further analyzed the expression array data. In the commonly duplicated regions, we found four cyclin genes, CYCD3;1 (At4g34160), CYCB1;1 (At4g37490), CYCB2;2 (At4g35620) and CYCD5;1 (At4g37630) (Vandepoele et al., 2002) ... The expression of these genes in at least two gra-D mutants was more than 1.36-fold higher than that in wild-type (P < 0.05, Table S1)

Horiguchi G, Gonzalez N, Beemster GT, Inzé D, Tsukaya H - Impact of segmental chromosomal duplications on leaf size in the grandifolia-D mutants of Arabidopsis thaliana

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Gene expression

a bHLH transcription factor BEE2 (At4g36540) (Friedrichsen et al., 2002) that positively controls brassinosteroid signaling, showed elevated expression levels in all three gra-D mutants (P < 0.05, Table S1)

Horiguchi G, Gonzalez N, Beemster GT, Inzé D, Tsukaya H - Impact of segmental chromosomal duplications on leaf size in the grandifolia-D mutants of Arabidopsis thaliana

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Gene expression

ANT ... expression ... in at least two gra-D mutants was more than 1.36-fold higher than that in wild-type (P < 0.05, Table S1

Horiguchi G, Gonzalez N, Beemster GT, Inzé D, Tsukaya H - Impact of segmental chromosomal duplications on leaf size in the grandifolia-D mutants of Arabidopsis thaliana

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Gene expression

in the gra-D mutants ... the expression of ... TUB4 on chromosome 5 were normal

Horiguchi G, Gonzalez N, Beemster GT, Inzé D, Tsukaya H - Impact of segmental chromosomal duplications on leaf size in the grandifolia-D mutants of Arabidopsis thaliana

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Gene expression

SMP2 ... expression ... in at least two gra-D mutants was more than 1.36-fold higher than that in wild-type (P < 0.05, Table S1

Horiguchi G, Gonzalez N, Beemster GT, Inzé D, Tsukaya H - Impact of segmental chromosomal duplications on leaf size in the grandifolia-D mutants of Arabidopsis thaliana

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Gene expression

we further analyzed the expression array data. In the commonly duplicated regions, we found four cyclin genes, CYCD3;1 (At4g34160), CYCB1;1 (At4g37490), CYCB2;2 (At4g35620) and CYCD5;1 (At4g37630) (Vandepoele et al., 2002) ... The expression of these genes in at least two gra-D mutants was more than 1.36-fold higher than that in wild-type (P < 0.05, Table S1)

Horiguchi G, Gonzalez N, Beemster GT, Inzé D, Tsukaya H - Impact of segmental chromosomal duplications on leaf size in the grandifolia-D mutants of Arabidopsis thaliana

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Gene expression

a brassinosteroid-signaling kinase BSK1 (At4g35230) (Tang et al., 2008) ... showed elevated expression levels in all three gra-D mutants (P < 0.05, Table S1)

Horiguchi G, Gonzalez N, Beemster GT, Inzé D, Tsukaya H - Impact of segmental chromosomal duplications on leaf size in the grandifolia-D mutants of Arabidopsis thaliana

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Gene expression

we further analyzed the expression array data. In the commonly duplicated regions, we found four cyclin genes, CYCD3;1 (At4g34160), CYCB1;1 (At4g37490), CYCB2;2 (At4g35620) and CYCD5;1 (At4g37630) (Vandepoele et al., 2002) ... The expression of these genes in at least two gra-D mutants was more than 1.36-fold higher than that in wild-type (P < 0.05, Table S1)

Horiguchi G, Gonzalez N, Beemster GT, Inzé D, Tsukaya H - Impact of segmental chromosomal duplications on leaf size in the grandifolia-D mutants of Arabidopsis thaliana

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Gene expression

At4g37760 ... was also increased in the gra-D mutants

Horiguchi G, Gonzalez N, Beemster GT, Inzé D, Tsukaya H - Impact of segmental chromosomal duplications on leaf size in the grandifolia-D mutants of Arabidopsis thaliana

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Gene expression

the brassinosteroid receptor BRI1 (At4g39400) (Li and Chory, 1997) ... showed elevated expression levels in all three gra-D mutants (P < 0.05, Table S1)

Horiguchi G, Gonzalez N, Beemster GT, Inzé D, Tsukaya H - Impact of segmental chromosomal duplications on leaf size in the grandifolia-D mutants of Arabidopsis thaliana

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Gene expression

RT-PCR analysis confirmed that the ANT ... transcripts were more abundant in the gra-D mutants than in the wild-type

Horiguchi G, Gonzalez N, Beemster GT, Inzé D, Tsukaya H - Impact of segmental chromosomal duplications on leaf size in the grandifolia-D mutants of Arabidopsis thaliana

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Gene expression

in the gra-D mutants ... the expression of ARGOS, which acts upstream of ANT (Hu et al., 2003) and is located on chromosome 3 ... were normal

Horiguchi G, Gonzalez N, Beemster GT, Inzé D, Tsukaya H - Impact of segmental chromosomal duplications on leaf size in the grandifolia-D mutants of Arabidopsis thaliana

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Gene expression

RT-PCR analysis confirmed that the ... CYCD3;1 transcripts were more abundant in the gra-D mutants than in the wild-type

Horiguchi G, Gonzalez N, Beemster GT, Inzé D, Tsukaya H - Impact of segmental chromosomal duplications on leaf size in the grandifolia-D mutants of Arabidopsis thaliana

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Gene expression

we further analyzed the expression array data. In the commonly duplicated regions, we found four cyclin genes, CYCD3;1 (At4g34160), CYCB1;1 (At4g37490), CYCB2;2 (At4g35620) and CYCD5;1 (At4g37630) (Vandepoele et al., 2002) ... The expression of these genes in at least two gra-D mutants was more than 1.36-fold higher than that in wild-type (P < 0.05, Table S1)

Horiguchi G, Gonzalez N, Beemster GT, Inzé D, Tsukaya H - Impact of segmental chromosomal duplications on leaf size in the grandifolia-D mutants of Arabidopsis thaliana

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Gene expression

Immunoblot analyses ... against ... D2 ... dramatically reduced

Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis

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Gene expression

the expression of ... ndhG ... reduced in emb1303-2

Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis

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Gene expression

GUS) staining on transgenic plants expressing GUS driven by the EMB1303 promoter ... GUS activity was ... low in stems

Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis

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Gene expression

Semi-quantitative RT-PCR analysis showed that the levels of ... GGRS transcripts were greatly decreased in emb1303-2

Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis

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Gene expression

the expression of ... rbcL ... reduced in emb1303-2

Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis

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Gene expression

the expression of ... psaA ... reduced in emb1303-2

Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis

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Gene expression

the expression of ... psbG ... reduced in emb1303-2

Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis

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Gene expression

Semi-quantitative RT-PCR analysis showed that the levels of PORB ... transcripts were greatly decreased in emb1303-2

Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis

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Gene expression

the expression of ... psbD ... reduced in emb1303-2

Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis

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Gene expression

the expression of ... psbE ... reduced in emb1303-2

Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis

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Gene expression

we analyzed protein profile of emb1303-2. A significant reduction of the large subunit of Rubisco (RBCL) was observed by Coomassie brilliant blue staining

Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis

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Gene expression

Immunoblot analyses ... against ... AtpB ... dramatically reduced

Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis

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Gene expression

GUS) staining on transgenic plants expressing GUS driven by the EMB1303 promoter ... GUS activity was high in ... young rosette leaves

Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis

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Gene expression

the expression of ... psbB ... reduced in emb1303-2

Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis

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Gene expression

Immunoblot analyses ... against ... LHCII ... barely detectable in emb1303-2

Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis

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Gene expression

Semi-quantitative RT-PCR analysis showed that the levels of ... PORC ... transcripts were greatly decreased in emb1303-2

Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis

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Gene expression

the expression of ... psbT ... reduced in emb1303-2

Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis

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Gene expression

the expression of ... psbA ... reduced in emb1303-2

Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis

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Gene expression

Semi-quantitative RT-PCR analysis ... CLA1 ... expression was also reduced in the mutant

Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis

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Gene expression

Immunoblot analyses ... against ... PsbO ... dramatically reduced

Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis

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Gene expression

GUS) staining on transgenic plants expressing GUS driven by the EMB1303 promoter ... GUS activity was high in cotyledons

Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis

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Gene expression

the expression of ... psaB ... reduced in emb1303-2

Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis

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Gene expression

transcript levels of accD ... remained the same in the mutant

Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis

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Gene expression

Immunoblot analyses ... against ... D1 ... barely detectable in emb1303-2

Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis

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Gene expression

Semi-quantitative RT-PCR analysis showed that the levels of ... CAO ... transcripts were greatly decreased in emb1303-2

Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis

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Gene expression

Total RNA was isolated from the first two leaves of 6-d-old wild-type and gif1/2/3 triple mutant plants, in which their cell proliferation occurred at a maximum rate, and subjected to RT-PCR. The transcript levels of ... CycB1;1 ... were significantly reduced in the triple mutant

Lee BH, Ko JH, Lee S, Lee Y, Pak JH, Kim JH - The Arabidopsis GRF-INTERACTING FACTOR gene family performs an overlapping function in determining organ size as well as multiple developmental properties

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Gene expression

Total RNA was isolated from the first two leaves of 6-d-old wild-type and gif1/2/3 triple mutant plants, in which their cell proliferation occurred at a maximum rate, and subjected to RT-PCR. The transcript levels of ... CycD3;1 ... were significantly reduced in the triple mutant

Lee BH, Ko JH, Lee S, Lee Y, Pak JH, Kim JH - The Arabidopsis GRF-INTERACTING FACTOR gene family performs an overlapping function in determining organ size as well as multiple developmental properties

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Gene expression

Total RNA was isolated from the first two leaves of 6-d-old wild-type and gif1/2/3 triple mutant plants, in which their cell proliferation occurred at a maximum rate, and subjected to RT-PCR. The transcript levels of ... a cyclin-dependent kinase gene, Cdc2b, were significantly reduced in the triple mutant

Lee BH, Ko JH, Lee S, Lee Y, Pak JH, Kim JH - The Arabidopsis GRF-INTERACTING FACTOR gene family performs an overlapping function in determining organ size as well as multiple developmental properties

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Gene expression

Total RNA was isolated from the first two leaves of 6-d-old wild-type and gif1/2/3 triple mutant plants, in which their cell proliferation occurred at a maximum rate, and subjected to RT-PCR ... The S-phase-specific proliferating cell nuclear antigen PCNA1 ... was remarkably down-regulated as well

Lee BH, Ko JH, Lee S, Lee Y, Pak JH, Kim JH - The Arabidopsis GRF-INTERACTING FACTOR gene family performs an overlapping function in determining organ size as well as multiple developmental properties

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Gene expression

In Arabidopsis, organ polarity regulation has been characterized extensively at the molecular level. We therefore have monitored the expression levels of a selection of Arabidopsis genes known to be involved in abaxial/adaxial patterning. The selection of genes comprises ... AS2 ... None of the monitored Arabidopsis genes exhibited significant changes in transcript levels between wox1 prs mutant samples and the wild type

Vandenbussche M, Horstman A, Zethof J, Koes R, Rijpkema AS, Gerats T - Differential recruitment of WOX transcription factors for lateral development and organ fusion in Petunia and Arabidopsis

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Gene expression

In Arabidopsis, organ polarity regulation has been characterized extensively at the molecular level. We therefore have monitored the expression levels of a selection of Arabidopsis genes known to be involved in abaxial/adaxial patterning. The selection of genes comprises ... ASYMMETRIC LEAVES1 (AS1 ... None of the monitored Arabidopsis genes exhibited significant changes in transcript levels between wox1 prs mutant samples and the wild type

Vandenbussche M, Horstman A, Zethof J, Koes R, Rijpkema AS, Gerats T - Differential recruitment of WOX transcription factors for lateral development and organ fusion in Petunia and Arabidopsis

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Gene expression

In Arabidopsis, organ polarity regulation has been characterized extensively at the molecular level. We therefore have monitored the expression levels of a selection of Arabidopsis genes known to be involved in abaxial/adaxial patterning. The selection of genes comprises ... REVOLUTA ... None of the monitored Arabidopsis genes exhibited significant changes in transcript levels between wox1 prs mutant samples and the wild type

Vandenbussche M, Horstman A, Zethof J, Koes R, Rijpkema AS, Gerats T - Differential recruitment of WOX transcription factors for lateral development and organ fusion in Petunia and Arabidopsis

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Gene expression

In Arabidopsis, organ polarity regulation has been characterized extensively at the molecular level. We therefore have monitored the expression levels of a selection of Arabidopsis genes known to be involved in abaxial/adaxial patterning. The selection of genes comprises ... KANADI (KAN ... None of the monitored Arabidopsis genes exhibited significant changes in transcript levels between wox1 prs mutant samples and the wild type

Vandenbussche M, Horstman A, Zethof J, Koes R, Rijpkema AS, Gerats T - Differential recruitment of WOX transcription factors for lateral development and organ fusion in Petunia and Arabidopsis

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Gene expression

In Arabidopsis, organ polarity regulation has been characterized extensively at the molecular level. We therefore have monitored the expression levels of a selection of Arabidopsis genes known to be involved in abaxial/adaxial patterning. The selection of genes comprises ... ETTIN (ETT ... None of the monitored Arabidopsis genes exhibited significant changes in transcript levels between wox1 prs mutant samples and the wild type

Vandenbussche M, Horstman A, Zethof J, Koes R, Rijpkema AS, Gerats T - Differential recruitment of WOX transcription factors for lateral development and organ fusion in Petunia and Arabidopsis

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Gene expression

None of the monitored Arabidopsis genes exhibited significant changes in transcript levels between wox1 prs mutant samples and the wild type, except for the ARF4 gene, which was moderately upregulated in wox1 prs mutants (Figure 6A ). Although only a subtle effect, upregulation of the abaxial cell fate–promoting ARF4 gene is consistent with our morphological observations

Vandenbussche M, Horstman A, Zethof J, Koes R, Rijpkema AS, Gerats T - Differential recruitment of WOX transcription factors for lateral development and organ fusion in Petunia and Arabidopsis

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Gene expression

In Arabidopsis, organ polarity regulation has been characterized extensively at the molecular level. We therefore have monitored the expression levels of a selection of Arabidopsis genes known to be involved in abaxial/adaxial patterning. The selection of genes comprises FILAMENTOUS FLOWER/YABBY1 ... YAB1 ... None of the monitored Arabidopsis genes exhibited significant changes in transcript levels between wox1 prs mutant samples and the wild type

Vandenbussche M, Horstman A, Zethof J, Koes R, Rijpkema AS, Gerats T - Differential recruitment of WOX transcription factors for lateral development and organ fusion in Petunia and Arabidopsis

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Gene expression

At2g39360 ... slightly upregulated by BL treatment in the wild-type

Guo H, Ye H, Li L, Yin Y - A family of receptor-like kinases are regulated by BES1 and involved in plant growth in Arabidopsis thaliana

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Gene expression

At2g39360 ... more clearly increased in bes1-D mutant

Guo H, Ye H, Li L, Yin Y - A family of receptor-like kinases are regulated by BES1 and involved in plant growth in Arabidopsis thaliana

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Gene expression

At5g24010 ... slightly upregulated by BL treatment in the wild-type

Guo H, Ye H, Li L, Yin Y - A family of receptor-like kinases are regulated by BES1 and involved in plant growth in Arabidopsis thaliana

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Gene expression

At1g30570 (HERK2 ... slightly upregulated by BL treatment in the wild-type

Guo H, Ye H, Li L, Yin Y - A family of receptor-like kinases are regulated by BES1 and involved in plant growth in Arabidopsis thaliana

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Gene expression

At1g30570 (HERK2 ... more clearly increased in bes1-D mutant

Guo H, Ye H, Li L, Yin Y - A family of receptor-like kinases are regulated by BES1 and involved in plant growth in Arabidopsis thaliana

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Gene expression

At5g24010 ... more clearly increased in bes1-D mutant

Guo H, Ye H, Li L, Yin Y - A family of receptor-like kinases are regulated by BES1 and involved in plant growth in Arabidopsis thaliana

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Gene expression

MYB23 (At5g40330 ... downregulated in the ang3 mutant

Van Minnebruggen A, Neyt P, De Groeve S, Coussens G, Ponce MR, Micol JL, Van Lijsebettens M - The ang3 mutation identified the ribosomal protein gene RPL5B with a role in cell expansion during organ growth

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Gene expression

We analyzed the levels of AtIPT7 transcripts in the above-ground parts of 10-day-old ... plants by real-time qRT-PCR ... the level of the transcript was 1.5-fold higher in 35S:BP plants than in the wild-type plants

Ikezaki M, Kojima M, Sakakibara H, Kojima S, Ueno Y, Machida C, Machida Y - Genetic networks regulated by ASYMMETRIC LEAVES1 (AS1) and AS2 in leaf development in Arabidopsis thaliana: KNOX genes control five morphological events

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Gene expression

In as1 ... levels of ETT/ARF3 transcripts are elevated ... We analyzed the levels of ETT transcripts in the shoot apices of as1-1 ... mutant plants by real-time qRT-PCR

Ikezaki M, Kojima M, Sakakibara H, Kojima S, Ueno Y, Machida C, Machida Y - Genetic networks regulated by ASYMMETRIC LEAVES1 (AS1) and AS2 in leaf development in Arabidopsis thaliana: KNOX genes control five morphological events

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Gene expression

the level of AtGA20ox1 transcripts in as1-1 plants was only 50% of that in the wild type

Ikezaki M, Kojima M, Sakakibara H, Kojima S, Ueno Y, Machida C, Machida Y - Genetic networks regulated by ASYMMETRIC LEAVES1 (AS1) and AS2 in leaf development in Arabidopsis thaliana: KNOX genes control five morphological events

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Gene expression

In as1 and as2 mutants, levels of ETT/ARF3 transcripts are elevated (Iwakawa et al., 2007; Figure 6l). We analyzed the levels of ETT transcripts in the shoot apices of ... as1-1 knat126 ... mutant plants by real-time qRT-PCR. As shown in Figure 6(l), the levels of ETT transcripts were similar in all plants examined, suggesting that the elevated levels of ETT transcripts in as1-1 and as2-1 plants were not caused by the ectopically expressed KNAT126 genes

Ikezaki M, Kojima M, Sakakibara H, Kojima S, Ueno Y, Machida C, Machida Y - Genetic networks regulated by ASYMMETRIC LEAVES1 (AS1) and AS2 in leaf development in Arabidopsis thaliana: KNOX genes control five morphological events

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Gene expression

We analyzed the levels of AtIPT7 transcripts in the above-ground parts of 10-day-old as1-1 mutant ... by real-time qRT-PCR. As shown in Figure 5(g), there was no obvious difference, in terms of the level of the AtIPT7 transcript, between wild-type and mutant plants

Ikezaki M, Kojima M, Sakakibara H, Kojima S, Ueno Y, Machida C, Machida Y - Genetic networks regulated by ASYMMETRIC LEAVES1 (AS1) and AS2 in leaf development in Arabidopsis thaliana: KNOX genes control five morphological events

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Gene expression

We analyzed the levels of AtGA20ox1 transcripts in the aerial parts of ... as1-1 plus knat126 mutant plants by real-time quantitative RT-PCR ... When a mutation in each KNAT126 gene was introduced separately into as1-1 plants, the level of the transcript returned to close to control values. These results suggest that the ectopically expressed KNAT126 genes in as1-1 plants might repress the expression of AtGA20ox1 in a redundant manner

Ikezaki M, Kojima M, Sakakibara H, Kojima S, Ueno Y, Machida C, Machida Y - Genetic networks regulated by ASYMMETRIC LEAVES1 (AS1) and AS2 in leaf development in Arabidopsis thaliana: KNOX genes control five morphological events

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Gene expression

In ... as2 mutants, levels of ETT/ARF3 transcripts are elevated ... We analyzed the levels of ETT transcripts in the shoot apices of ... as2-1 ... mutant plants by real-time qRT-PCR

Ikezaki M, Kojima M, Sakakibara H, Kojima S, Ueno Y, Machida C, Machida Y - Genetic networks regulated by ASYMMETRIC LEAVES1 (AS1) and AS2 in leaf development in Arabidopsis thaliana: KNOX genes control five morphological events

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Gene expression

We analyzed the levels of AtIPT7 transcripts in the above-ground parts of 10-day-old ... as1-1 plus knat126 mutant plants by real-time qRT-PCR. As shown in Figure 5(g), there was no obvious difference, in terms of the level of the AtIPT7 transcript, between wild-type and mutant plants

Ikezaki M, Kojima M, Sakakibara H, Kojima S, Ueno Y, Machida C, Machida Y - Genetic networks regulated by ASYMMETRIC LEAVES1 (AS1) and AS2 in leaf development in Arabidopsis thaliana: KNOX genes control five morphological events

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Gene expression

In as1 and as2 mutants, levels of ETT/ARF3 transcripts are elevated (Iwakawa et al., 2007; Figure 6l). We analyzed the levels of ETT transcripts in the shoot apices of ... as2-1 knat126 mutant plants by real-time qRT-PCR. As shown in Figure 6(l), the levels of ETT transcripts were similar in all plants examined, suggesting that the elevated levels of ETT transcripts in as1-1 and as2-1 plants were not caused by the ectopically expressed KNAT126 genes

Ikezaki M, Kojima M, Sakakibara H, Kojima S, Ueno Y, Machida C, Machida Y - Genetic networks regulated by ASYMMETRIC LEAVES1 (AS1) and AS2 in leaf development in Arabidopsis thaliana: KNOX genes control five morphological events

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Gene expression

To determine the expression pattern of SMO2, we generated transgenic plants expressing a pSMO2:GUS fusion gene and examined GUS activities in seedlings and developing organs ... strong GUS staining was observed ... in leaf

Hu Z, Qin Z, Wang M, Xu C, Feng G, Liu J, Meng Z, Hu Y - The Arabidopsis SMO2, a homologue of yeast TRM112, modulates progression of cell division during organ growth

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Gene expression

To determine the expression pattern of SMO2, we generated transgenic plants expressing a pSMO2:GUS fusion gene and examined GUS activities in seedlings and developing organs ... strong GUS staining was observed in shoot ... meristem

Hu Z, Qin Z, Wang M, Xu C, Feng G, Liu J, Meng Z, Hu Y - The Arabidopsis SMO2, a homologue of yeast TRM112, modulates progression of cell division during organ growth

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Gene expression

we ... examined the expression of five cell cycle checkpoint-related genes in WT and smo2, including ... HISTONE H4 ... by real-time quantitative reverse-transcriptional polymerase chain reaction (qRT-PCR), and found that the expression ... was elevated in the smo2 mutant

Hu Z, Qin Z, Wang M, Xu C, Feng G, Liu J, Meng Z, Hu Y - The Arabidopsis SMO2, a homologue of yeast TRM112, modulates progression of cell division during organ growth

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Gene expression

we ... examined the expression of five cell cycle checkpoint-related genes in WT and smo2, including ... CYCA1;1 ... by real-time quantitative reverse-transcriptional polymerase chain reaction (qRT-PCR), and found that the expression ... was elevated in the smo2 mutant

Hu Z, Qin Z, Wang M, Xu C, Feng G, Liu J, Meng Z, Hu Y - The Arabidopsis SMO2, a homologue of yeast TRM112, modulates progression of cell division during organ growth

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Gene expression

No transcript of At1g22270 was detected in smo2 plants by RT-PCR analysis

Hu Z, Qin Z, Wang M, Xu C, Feng G, Liu J, Meng Z, Hu Y - The Arabidopsis SMO2, a homologue of yeast TRM112, modulates progression of cell division during organ growth

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Gene expression

we introduced a pCYCB1;1:Dbox-GUS construct into smo2 ... the most dramatic accumulation of CYCB1;1:GUS protein was observed in smo2 ... juvenile leaves

Hu Z, Qin Z, Wang M, Xu C, Feng G, Liu J, Meng Z, Hu Y - The Arabidopsis SMO2, a homologue of yeast TRM112, modulates progression of cell division during organ growth

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Gene expression

we introduced a pCYCB1;1:Dbox-GUS construct into smo2 ... the most dramatic accumulation of CYCB1;1:GUS protein was observed in smo2 leaf primordia

Hu Z, Qin Z, Wang M, Xu C, Feng G, Liu J, Meng Z, Hu Y - The Arabidopsis SMO2, a homologue of yeast TRM112, modulates progression of cell division during organ growth

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Gene expression

we ... examined the expression of five cell cycle checkpoint-related genes in WT and smo2, including ... CYCB1;1, by real-time quantitative reverse-transcriptional polymerase chain reaction (qRT-PCR), and found that the expression ... was elevated in the smo2 mutant

Hu Z, Qin Z, Wang M, Xu C, Feng G, Liu J, Meng Z, Hu Y - The Arabidopsis SMO2, a homologue of yeast TRM112, modulates progression of cell division during organ growth

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Gene expression

To determine the expression pattern of SMO2, we generated transgenic plants expressing a pSMO2:GUS fusion gene and examined GUS activities in seedlings and developing organs ... a moderate level of GUS expression was detected in the cotyledon vascular bundles

Hu Z, Qin Z, Wang M, Xu C, Feng G, Liu J, Meng Z, Hu Y - The Arabidopsis SMO2, a homologue of yeast TRM112, modulates progression of cell division during organ growth

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Gene expression

we ... examined the expression of five cell cycle checkpoint-related genes in WT and smo2, including ... CYCB2;3 ... by real-time quantitative reverse-transcriptional polymerase chain reaction (qRT-PCR), and found that the expression ... was elevated in the smo2 mutant

Hu Z, Qin Z, Wang M, Xu C, Feng G, Liu J, Meng Z, Hu Y - The Arabidopsis SMO2, a homologue of yeast TRM112, modulates progression of cell division during organ growth

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Gene expression

we ... examined the expression of five cell cycle checkpoint-related genes in WT and smo2, including CYCD3;1 ... by real-time quantitative reverse-transcriptional polymerase chain reaction (qRT-PCR), and found that the expression ... was elevated in the smo2 mutant

Hu Z, Qin Z, Wang M, Xu C, Feng G, Liu J, Meng Z, Hu Y - The Arabidopsis SMO2, a homologue of yeast TRM112, modulates progression of cell division during organ growth

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Gene expression

FLC transcript levels were reduced in the ssrp1-2

Lolas IB, Himanen K, Grønlund JT, Lynggaard C, Houben A, Melzer M, Van Lijsebettens M, Grasser KD - The transcript elongation factor FACT affects Arabidopsis vegetative and reproductive development and genetically interacts with HUB1/2

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Gene expression

in the ... spt16-1 ... increased level of SOC1 transcript was detected

Lolas IB, Himanen K, Grønlund JT, Lynggaard C, Houben A, Melzer M, Van Lijsebettens M, Grasser KD - The transcript elongation factor FACT affects Arabidopsis vegetative and reproductive development and genetically interacts with HUB1/2

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Gene expression

in the ... spt16-2 ... increased level of SOC1 transcript was detected

Lolas IB, Himanen K, Grønlund JT, Lynggaard C, Houben A, Melzer M, Van Lijsebettens M, Grasser KD - The transcript elongation factor FACT affects Arabidopsis vegetative and reproductive development and genetically interacts with HUB1/2

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Gene expression

FLC transcript levels were reduced in the ... spt16-1

Lolas IB, Himanen K, Grønlund JT, Lynggaard C, Houben A, Melzer M, Van Lijsebettens M, Grasser KD - The transcript elongation factor FACT affects Arabidopsis vegetative and reproductive development and genetically interacts with HUB1/2

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Gene expression

the transcript level of SPT16 is not reduced in ssrp1-2

Lolas IB, Himanen K, Grønlund JT, Lynggaard C, Houben A, Melzer M, Van Lijsebettens M, Grasser KD - The transcript elongation factor FACT affects Arabidopsis vegetative and reproductive development and genetically interacts with HUB1/2

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Gene expression

The SSRP1 and SPT16 protein levels were also analysed by immunoblotting ... the amount of SPT16 was reduced in ... ssrp1-2

Lolas IB, Himanen K, Grønlund JT, Lynggaard C, Houben A, Melzer M, Van Lijsebettens M, Grasser KD - The transcript elongation factor FACT affects Arabidopsis vegetative and reproductive development and genetically interacts with HUB1/2

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Gene expression

FLC transcript levels were reduced in the ... spt16-2

Lolas IB, Himanen K, Grønlund JT, Lynggaard C, Houben A, Melzer M, Van Lijsebettens M, Grasser KD - The transcript elongation factor FACT affects Arabidopsis vegetative and reproductive development and genetically interacts with HUB1/2

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Gene expression

The SSRP1 and SPT16 protein levels were also analysed by immunoblotting ... The amount of SSRP1 was slightly reduced in the spt16 lines

Lolas IB, Himanen K, Grønlund JT, Lynggaard C, Houben A, Melzer M, Van Lijsebettens M, Grasser KD - The transcript elongation factor FACT affects Arabidopsis vegetative and reproductive development and genetically interacts with HUB1/2

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Gene expression

in the ssrp1-2 ... increased level of SOC1 transcript was detected

Lolas IB, Himanen K, Grønlund JT, Lynggaard C, Houben A, Melzer M, Van Lijsebettens M, Grasser KD - The transcript elongation factor FACT affects Arabidopsis vegetative and reproductive development and genetically interacts with HUB1/2

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Gene expression

SSRP1 is not reduced in spt16

Lolas IB, Himanen K, Grønlund JT, Lynggaard C, Houben A, Melzer M, Van Lijsebettens M, Grasser KD - The transcript elongation factor FACT affects Arabidopsis vegetative and reproductive development and genetically interacts with HUB1/2

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Gene expression

weak expression of miR396 at ... leaf primordia