ROT3P::GUS ... in leaves, histochemical analysis indicated that the ... expression ... was ubiquitous in all cell layers, including epidermis, palisade tissue, and the spongy layer
Kim GT, Tsukaya H, Saito Y, Uchimiya H - Changes in the shapes of leaves and flowers upon overexpression of cytochrome P450 in Arabidopsis
Visit the PubMed ArticleROT3P::GUS ... expression ... was ... in ... the spongy layer
Kim GT, Tsukaya H, Saito Y, Uchimiya H - Changes in the shapes of leaves and flowers upon overexpression of cytochrome P450 in Arabidopsis
Visit the PubMed ArticleROT3P::GUS ... we observed ... in leaves
Kim GT, Tsukaya H, Saito Y, Uchimiya H - Changes in the shapes of leaves and flowers upon overexpression of cytochrome P450 in Arabidopsis
Visit the PubMed ArticleROT3P::GUS ... expression ... was ... in ... epidermis
Kim GT, Tsukaya H, Saito Y, Uchimiya H - Changes in the shapes of leaves and flowers upon overexpression of cytochrome P450 in Arabidopsis
Visit the PubMed Articlein leaves, histochemical analysis indicated that the ROT3 promoter was expressed more strongly in distal, mature regions than in proximal, immature regions
Kim GT, Tsukaya H, Saito Y, Uchimiya H - Changes in the shapes of leaves and flowers upon overexpression of cytochrome P450 in Arabidopsis
Visit the PubMed ArticleROT3P::GUS ... expression ... was ... in ... palisade tissue
Kim GT, Tsukaya H, Saito Y, Uchimiya H - Changes in the shapes of leaves and flowers upon overexpression of cytochrome P450 in Arabidopsis
Visit the PubMed ArticleYAB3 is detected in the abaxial regions of the developing leaves
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleYAB2 also appears to be expressed abaxially in cotyledon primordia
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleFIL ... expression extending through more than half of the cell layers in leaf primordia
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleFIL, YAB2 and YAB3 are expressed in a qualitatively similar manner at the mRNA level, although expression levels differ dramatically ... FIL is expressed at a high level, YAB3 at a moderate level, and YAB2 at a low level
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleFIL ... Expression is initially in a small group of subepidermal cells in the central region of leaf anlagen
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleIn most leaf primordia of phb-1d homozygotes, FIL expression is undetectable
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleFIL ... expressed in abaxial regions of above ground lateral organ primordia
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleYAB2 ... expressed in abaxial regions of above ground lateral organ
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleFIL is initially expressed in the transition-stage embryo (between the late globular and heart stages) in a small number of subepidermal cells in the central region of the cotyledon anlagen
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleIn leaves, FIL is more broadly ... expressed
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleIn phb-1d/+ heterozygotes ... FIL expression domain is reduced, usually consisting of only a couple of cell layers on the abaxial side of the leaf primordium (Fig. 5B), rather than in four cell layers as observed in wild type
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed Articlein developing embryos ... initial detection of YAB3 is at the early heart stage
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleYAB3 ... expressed in abaxial regions of above ground lateral organ primordia
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleYAB3 ... Expression ... appears to be in ... three layers of spongy mesophyll
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleFIL ... No signal is detected in differentiated leaves
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed Articlein leaf primordium ... As the central cells begin differentiating, FIL expression declines, but remains high in the marginal abaxial regions
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleIn differentiating leaves FIL mRNA can be detected ... in layers destined to become spongy mesophyll
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleIn differentiating leaves FIL mRNA can be detected in the abaxial epidermis
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleFIL, YAB2 and YAB3 are expressed in a qualitatively similar manner at the mRNA level, although expression levels differ dramatically ... FIL is expressed at a high level, YAB3 at a moderate level, and YAB2 at a low level
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleYAB3 ... expressed in abaxial regions of ... cotyledons
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleExpression of YAB2 ... is detected in the abaxial regions of the developing leaves
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleIn differentiating leaves FIL mRNA ... in the ... adaxial epidermis expression ... is undetectable
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleIn phb-1d/+ heterozygotes FIL expression is detected in most leaf primordia
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleFIL ... expression is throughout the abaxial domain of the cotyledon ... primordia ... walking-stick ... stages
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleYAB2 ... expressed in abaxial regions of ... cotyledons
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleFIL ... expressed in abaxial regions of ... cotyledons
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleFIL ... expression is throughout the abaxial domain of the cotyledon primordia ... U ... - ... shaped stages
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleAs leaves fully differentiate, YAB3 expression declines to an undetectable level
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleYAB3 expression ... with initial expression occurring in leaf anlagen
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleFIL ... expressed in abaxial regions of ... leaves
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleFIL ... As leaf primordia emerge, expression becomes restricted to the abaxial regions
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleFIL ... By mid-heart stage expression is throughout the abaxial domain of the cotyledon primordia
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleIn differentiating leaves FIL mRNA ... in the palisade mesophyll ... expression ... is undetectable
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleYAB2 ... expressed in abaxial regions of ... leaves
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleFIL, YAB2 and YAB3 are expressed in a qualitatively similar manner at the mRNA level, although expression levels differ dramatically ... FIL is expressed at a high level, YAB3 at a moderate level, and YAB2 at a low level
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleYAB3 ... in developing embryos ... expressed abaxially in cotyledon primordia
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleFIL expression is detectable in at least two incipient leaf primordia prior to their emergence from the flanks of the apical meristem
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleFIL ... gradually fades as the embryos mature
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleFIL expression is not altered in ... leaves ... of yab3-1 mutants
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleYAB3 ... Expression appears to be in ... the abaxial epidermis
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleYAB3 ... expressed in abaxial regions of ... leaves
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleYAB3 ... As leaf primordia emerge from the apical meristem, expression is confined to the abaxial regions of developing primordia
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleFIL ... By early heart stage expression has expanded to include cells on the abaxial side of the emergent cotyledon primordia, but does not immediately extend to the tip of the cotyledon primordia
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleFIL ... expression is throughout the abaxial domain of the cotyledon ... primordia ... torpedo ... stages
Siegfried KR, Eshed Y, Baum SF, Otsuga D, Drews GN, Bowman JL - Members of the YABBY gene family specify abaxial cell fate in Arabidopsis
Visit the PubMed ArticleCycD3 transcripts were still observed in 35S::ANT leaves, but not in control
Mizukami Y, Fischer RL - Plant organ size control: AINTEGUMENTA regulates growth and cell numbers during organogenesis
Visit the PubMed ArticleANT mRNA accumulated in leaf
Mizukami Y, Fischer RL - Plant organ size control: AINTEGUMENTA regulates growth and cell numbers during organogenesis
Visit the PubMed ArticleWe estimate ... ≈35-fold more AtEXP10 mRNA in young petioles as compared with corresponding mature tissues
Cho HT, Cosgrove DJ - Altered expression of expansin modulates leaf growth and pedicel abscission in Arabidopsis thaliana
Visit the PubMed ArticleAs leaf development progressed, AtEXP10::GUS expression began in the base of the petiole and gradually extended toward the whole midrib
Cho HT, Cosgrove DJ - Altered expression of expansin modulates leaf growth and pedicel abscission in Arabidopsis thaliana
Visit the PubMed ArticleThe first expression detectable by AtEXP10::GUS occurred at the base of the emerging first two true leaves but not of the cotyledons
Cho HT, Cosgrove DJ - Altered expression of expansin modulates leaf growth and pedicel abscission in Arabidopsis thaliana
Visit the PubMed ArticleAtEXP10::GUS ... low level of staining in the shoot meristem was observed
Cho HT, Cosgrove DJ - Altered expression of expansin modulates leaf growth and pedicel abscission in Arabidopsis thaliana
Visit the PubMed ArticleAtEXP10::GUS expression ... disappeared altogether as the leaf matured
Cho HT, Cosgrove DJ - Altered expression of expansin modulates leaf growth and pedicel abscission in Arabidopsis thaliana
Visit the PubMed ArticleAtEXP10::GUS expression ... later ... was restricted to the vasculature of the petiole and leaf blade
Cho HT, Cosgrove DJ - Altered expression of expansin modulates leaf growth and pedicel abscission in Arabidopsis thaliana
Visit the PubMed ArticleAtEXP10::GUS also was notably expressed in growing trichomes
Cho HT, Cosgrove DJ - Altered expression of expansin modulates leaf growth and pedicel abscission in Arabidopsis thaliana
Visit the PubMed ArticleWe estimate ≈200-fold more AtEXP10 mRNA in young leaf blades ... as compared with corresponding mature tissues
Cho HT, Cosgrove DJ - Altered expression of expansin modulates leaf growth and pedicel abscission in Arabidopsis thaliana
Visit the PubMed ArticleLEP expression was significantly increased in let compared to wild type
van der Graaff E, Dulk-Ras AD, Hooykaas PJ, Keller B - Activation tagging of the LEAFY PETIOLE gene affects leaf petiole development in Arabidopsis thaliana
Visit the PubMed ArticleLEP is only expressed in young shoots of wild-type plants
van der Graaff E, Dulk-Ras AD, Hooykaas PJ, Keller B - Activation tagging of the LEAFY PETIOLE gene affects leaf petiole development in Arabidopsis thaliana
Visit the PubMed ArticleIn wild type no LEP expression could be detected in the cotyledons
van der Graaff E, Dulk-Ras AD, Hooykaas PJ, Keller B - Activation tagging of the LEAFY PETIOLE gene affects leaf petiole development in Arabidopsis thaliana
Visit the PubMed Articledetected LEP expression in stipules
van der Graaff E, Dulk-Ras AD, Hooykaas PJ, Keller B - Activation tagging of the LEAFY PETIOLE gene affects leaf petiole development in Arabidopsis thaliana
Visit the PubMed ArticleIn wild type no LEP expression could be detected in the ... non-expanding (old) leaves
van der Graaff E, Dulk-Ras AD, Hooykaas PJ, Keller B - Activation tagging of the LEAFY PETIOLE gene affects leaf petiole development in Arabidopsis thaliana
Visit the PubMed ArticleLEP expression ... restricted to the leaf blades
van der Graaff E, Dulk-Ras AD, Hooykaas PJ, Keller B - Activation tagging of the LEAFY PETIOLE gene affects leaf petiole development in Arabidopsis thaliana
Visit the PubMed ArticleLEP expression was strongest in very young leaf blades and became weaker upon further expansion of these blades
van der Graaff E, Dulk-Ras AD, Hooykaas PJ, Keller B - Activation tagging of the LEAFY PETIOLE gene affects leaf petiole development in Arabidopsis thaliana
Visit the PubMed ArticleIn let ... LEP expression was detected in all organs analysed
van der Graaff E, Dulk-Ras AD, Hooykaas PJ, Keller B - Activation tagging of the LEAFY PETIOLE gene affects leaf petiole development in Arabidopsis thaliana
Visit the PubMed ArticleIn wild type, LEP was expressed throughout very young leaf primordia
van der Graaff E, Dulk-Ras AD, Hooykaas PJ, Keller B - Activation tagging of the LEAFY PETIOLE gene affects leaf petiole development in Arabidopsis thaliana
Visit the PubMed ArticleLEP was very weakly expressed in wild type and could only be detected in young shoots
van der Graaff E, Dulk-Ras AD, Hooykaas PJ, Keller B - Activation tagging of the LEAFY PETIOLE gene affects leaf petiole development in Arabidopsis thaliana
Visit the PubMed ArticleLEP ... staining in all cells constituting the young leaf primordia
van der Graaff E, Dulk-Ras AD, Hooykaas PJ, Keller B - Activation tagging of the LEAFY PETIOLE gene affects leaf petiole development in Arabidopsis thaliana
Visit the PubMed ArticleIn ... 35SDE-LEP overexpressors LEP expression was detected in all organs analysed
van der Graaff E, Dulk-Ras AD, Hooykaas PJ, Keller B - Activation tagging of the LEAFY PETIOLE gene affects leaf petiole development in Arabidopsis thaliana
Visit the PubMed ArticleLEP expression could also be detected in young developing leaves
van der Graaff E, Dulk-Ras AD, Hooykaas PJ, Keller B - Activation tagging of the LEAFY PETIOLE gene affects leaf petiole development in Arabidopsis thaliana
Visit the PubMed Articleclf-18 ... leaves ... contain ... AP3 floral genes
Serrano-Cartagena J, Candela H, Robles P, Ponce MR, Pérez-Pérez JM, Piqueras P, Micol JL - Genetic analysis of incurvata mutants reveals three independent genetic operations at work in Arabidopsis leaf morphogenesis
Visit the PubMed Articleicu2 leaves ... contain ... AG ... floral genes
Serrano-Cartagena J, Candela H, Robles P, Ponce MR, Pérez-Pérez JM, Piqueras P, Micol JL - Genetic analysis of incurvata mutants reveals three independent genetic operations at work in Arabidopsis leaf morphogenesis
Visit the PubMed Articleclf-18 ... leaves ... contain ... AG ... floral genes
Serrano-Cartagena J, Candela H, Robles P, Ponce MR, Pérez-Pérez JM, Piqueras P, Micol JL - Genetic analysis of incurvata mutants reveals three independent genetic operations at work in Arabidopsis leaf morphogenesis
Visit the PubMed ArticleEctopic transcription of AP1 was detected in ... clf-61 allele
Serrano-Cartagena J, Candela H, Robles P, Ponce MR, Pérez-Pérez JM, Piqueras P, Micol JL - Genetic analysis of incurvata mutants reveals three independent genetic operations at work in Arabidopsis leaf morphogenesis
Visit the PubMed ArticleEctopic transcription of AP1 was detected in icu2
Serrano-Cartagena J, Candela H, Robles P, Ponce MR, Pérez-Pérez JM, Piqueras P, Micol JL - Genetic analysis of incurvata mutants reveals three independent genetic operations at work in Arabidopsis leaf morphogenesis
Visit the PubMed Articleicu2 leaves ... contain ... AP3 floral genes
Serrano-Cartagena J, Candela H, Robles P, Ponce MR, Pérez-Pérez JM, Piqueras P, Micol JL - Genetic analysis of incurvata mutants reveals three independent genetic operations at work in Arabidopsis leaf morphogenesis
Visit the PubMed ArticleUsing in situ hybridization ... SHOOTMERISTEMLESS (STM) ... as1 ... se ... strong expression in the meristem
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleUsing in situ hybridization, we could not detect SHOOTMERISTEMLESS (STM) expression in leaves of as1 ... se
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleKNAT1 ... as1 ... se ... expression was confined to the peripheral zone
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed Articleas1 se ... showed expression of KNAT1::GUS in leaves
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleKNAT2::GUS ... Leaves of ... as2 ... revealed GUS expression in the sinuses
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleKNAT2::GUS expression appeared normal in se mutants
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleKNAT1::GUS-19 expression in pkl-15 single mutants was indistinguishable from that of wild type
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed Articleas2 se ... showed expression of KNAT1::GUS in leaves
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleKNAT1::GUS-18 ... Weak GUS expression was also seen in the sinus of the occasional lobes that formed on as1 ... leaves
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleIn as2 mutants, KNAT1::GUS-1 expression was detected in the petioles of leaves
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleKNAT1::GUS-18 ... Weak GUS expression was also seen in the sinus of the occasional lobes that formed on ... as2 leaves
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleSporadic expression of KNAT1 was detected in the basal part of young leaves of ... as2 se mutants
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleKNAT1 ... as2 ... se ... expression was confined to the peripheral zone
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleSporadic expression of KNAT1 was detected in the basal part of young leaves of ... as2
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleIn as2 mutants, KNAT1::GUS-1 expression was detected ... in the main vein
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleExpression of KNAT1 RNA in meristems of ... as1 ... se ... appeared similar to that of wild- type plants
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleIn as2 mutants, KNAT1::GUS-1 expression was detected ... sometimes in secondary veins
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleKNAT1::GUS-19 ... GUS expression in as1-1 pkl-15 plants was ... less intense throughout the leaf
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleKNAT1::GUS-19 ... GUS expression in as1-1 pkl-15 plants was concentrated in the sinus region
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleKNAT1 ... as1 ... expression was confined to the peripheral zone
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleKNAT1::GUS-1 line was detected in the ... shoot meristems
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleSporadic expression of KNAT1 was detected in the basal part of young leaves of as1
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleKNAT1 ... as2 ... expression was confined to the peripheral zone
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleExpression of KNAT1 RNA in meristems of ... as1 ... appeared similar to that of wild- type plants
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleKNAT1 ... as1 ... se ... not detected ... in the youngest leaf primordia
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleKNAT2::GUS ... Leaves of ... as2 single mutants revealed GUS expression ... slightly in the basal portion of the leaf petioles
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleUsing in situ hybridization ... SHOOTMERISTEMLESS (STM) ... as1 ... strong expression in the meristem
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleUsing in situ hybridization, we could not detect SHOOTMERISTEMLESS (STM) expression in leaves of as1
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleExpression of KNAT1 RNA in meristems of ... as2 ... se ... appeared similar to that of wild- type plants
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleUsing in situ hybridization ... SHOOTMERISTEMLESS (STM) ... as2 ... strong expression in the meristem
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleUsing in situ hybridization, we could not detect SHOOTMERISTEMLESS (STM) expression in leaves of ... se
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed Articleas2 se ... showed expression of KNAT1::GUS in leaves
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleUsing in situ hybridization, we could not detect SHOOTMERISTEMLESS (STM) expression in leaves of ... as2
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleKNAT1::GUS-18 ... expression was strong ... in the vasculature of as1 leaves
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleKNAT2::GUS ... Leaves of as1 ... single mutants revealed GUS expression ... slightly in the basal portion of the leaf petioles
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed Articleas2 se double mutants expressed KNAT2::GUS strongly in the sinuses of lobes
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleKNAT1 ... as2 ... not detected ... in the youngest leaf primordia
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleUsing in situ hybridization ... SHOOTMERISTEMLESS (STM) ... as2 ... se ... strong expression in the meristem
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleIn wild-type plants carrying the KNAT2::GUS construct, staining was detected in meristems but not leaves
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleKNAT2::GUS ... Leaves of as1 ... revealed GUS expression in the sinuses
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed Articleas1 se ... double mutants expressed KNAT2::GUS strongly in the sinuses of lobes
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleUsing in situ hybridization ... SHOOTMERISTEMLESS (STM) ... se ... strong expression in the meristem
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleKNAT1::GUS-1 line was detected ... not in leaves
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleUsing in situ hybridization, we could not detect SHOOTMERISTEMLESS (STM) expression in leaves of ... as2 ... se
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleKNAT1 ... as1 ... not detected ... in the youngest leaf primordia
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleExpression of KNAT1 RNA in meristems of ... as2 ... appeared similar to that of wild- type plants
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleIn wild-type plants carrying the KNAT2::GUS construct, staining was detected in meristems
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleKNAT1 ... as2 ... se ... not detected ... in the youngest leaf primordia
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleIn as2 mutants, KNAT1::GUS-1 expression was detected ... occasionally in patches on the leaves
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleKNAT1::GUS-18 ... expression was strong in the petioles ... of as1 leaves
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed ArticleSporadic expression of KNAT1 was detected in the basal part of young leaves of ... as1 se
Ori N, Eshed Y, Chuck G, Bowman JL, Hake S - Mechanisms that control knox gene expression in the Arabidopsis shoot
Visit the PubMed Articletransgenic lines in which NAC1 was ... silenced ... DBP ... showed ... a reduction
Xie Q, Frugis G, Colgan D, Chua NH - Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development
Visit the PubMed Articleauxin treatment increased ... AIR3 expression levels in wild-type plants
Xie Q, Frugis G, Colgan D, Chua NH - Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development
Visit the PubMed ArticleWhole-mount in situ hybridization of 7–12-d-old seedlings detected ... tissues actively expressing the NAC1 gene ... the primordia of the developing leaves
Xie Q, Frugis G, Colgan D, Chua NH - Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development
Visit the PubMed Articletransgenic lines in which NAC1 was ... silenced ... AIR3 ... showed ... a reduction
Xie Q, Frugis G, Colgan D, Chua NH - Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development
Visit the PubMed Articletransgenic lines carrying a GFP-NAC1 fusion gene expressed under the control of a dexamethasone-inducible GVG promoter ... In the absence of dex, no fluorescence was observed (Fig. (Fig.2A,2A, panel 2), however, in the presence of dex, the GFP–NAC1 was localized exclusively to the nucleus
Xie Q, Frugis G, Colgan D, Chua NH - Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development
Visit the PubMed ArticleNAC1 was expressed at a moderate level in 2-wk-old seedlings, however, in dissected tissues of mature plants it was expressed at high levels in roots, at low levels in stems and leaves, and not at all in flowers or siliques
Xie Q, Frugis G, Colgan D, Chua NH - Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development
Visit the PubMed Articletransgenic lines in which NAC1 was overexpressed ... AIR3 ... showed an increase
Xie Q, Frugis G, Colgan D, Chua NH - Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development
Visit the PubMed ArticleWhole-mount in situ hybridization of 7–12-d-old seedlings detected ... tissues actively expressing the NAC1 gene: the expanding cotyledons
Xie Q, Frugis G, Colgan D, Chua NH - Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development
Visit the PubMed Articleauxin treatment increased ... NAC1 ... expression levels in wild-type plants
Xie Q, Frugis G, Colgan D, Chua NH - Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development
Visit the PubMed Articleconstruct containing a 2-kb DNA fragment upstream of the NAC1 coding region was fused to a β-glucuronidase (GUS) reporter gene, and several independent transgenic lines were analyzed ... In the leaf primordia, a signal, which diminished as the leaf matured, was also observed
Xie Q, Frugis G, Colgan D, Chua NH - Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development
Visit the PubMed Articlea construct containing a 2-kb DNA fragment upstream of the NAC1 coding region was fused to a β-glucuronidase (GUS) reporter gene, and several independent transgenic lines were analyzed ... moderate activity was observed in the cotyledons
Xie Q, Frugis G, Colgan D, Chua NH - Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development
Visit the PubMed Articletransgenic lines in which NAC1 was overexpressed ... DBP ... showed an increase
Xie Q, Frugis G, Colgan D, Chua NH - Arabidopsis NAC1 transduces auxin signal downstream of TIR1 to promote lateral root development
Visit the PubMed Article35S-ICK1 ... Expression of the CDK gene cdc2a did not decrease, and perhaps showed a slight increase
Wang H, Zhou Y, Gilmer S, Whitwill S, Fowke LC - Expression of the plant cyclin-dependent kinase inhibitor ICK1 affects cell division, plant growth and morphology
Visit the PubMed ArticleKNAT1 was ectopically expressed in as1 leaves
Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis
Visit the PubMed ArticleKNAT1 transcripts in wild-type plants were detected ... not in leaves
Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis
Visit the PubMed ArticleKNAT2 transcripts were present at high levels in wild-type shoot
Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis
Visit the PubMed Articleas1 showed misexpression of KNAT1
Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis
Visit the PubMed ArticleIn wild-type embryos AS1 RNA ... was absent or reduced in ... the cotyledon epidermis
Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis
Visit the PubMed ArticleSTM expression ... is absent from ... leaf primordia ... in as1
Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis
Visit the PubMed Articleas1 showed misexpression of ... KNAT2
Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis
Visit the PubMed Articlewild-type embryos AS1 RNA ... was absent or reduced in cells that would subsequently form the shoot apical meristem
Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis
Visit the PubMed ArticleSTM expression in the wild type ... is absent from ... leaf primordia
Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis
Visit the PubMed ArticleIn wild-type embryos AS1 RNA first became detectable in late globular stage, predominantly in two subepidermal domains corresponding to cotyledon initials
Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis
Visit the PubMed ArticleAfter germination, AS1 was detected in leaf founder cells ... until stage P4
Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis
Visit the PubMed ArticleKNAT2 transcripts were present ... at a low level in wild-type leaves
Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis
Visit the PubMed ArticleIn wild-type embryos AS1 RNA ... Expression was maintained in subepidermal cells of developing cotyledons from heart stage onwards
Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis
Visit the PubMed ArticleAfter germination, AS1 was detected ... in cells associated with the cotyledon vasculature
Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis
Visit the PubMed ArticleKNAT1 transcripts in wild-type plants were detected in whole-shoot
Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis
Visit the PubMed ArticleSTM expression in the wild type ... is absent from leaves
Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis
Visit the PubMed Articlein as1 ... STM expression was unchanged
Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis
Visit the PubMed ArticleSTM expression ... is absent from leaves ... in as1
Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis
Visit the PubMed ArticleSTM expression in the wild type is confined to SAM cells
Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis
Visit the PubMed ArticleKNAT1 ... was ectopically expressed in the cotyledons of as1 embryos
Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis
Visit the PubMed ArticleSTM ... expression ... is confined to SAM cells ... in as1
Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis
Visit the PubMed ArticleIn as1 mutant leaves, expression of KNAT2 was upregulated
Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis
Visit the PubMed Articlestm embryos revealed AS1 expression throughout the apical half of ... heart stage embryos
Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis
Visit the PubMed Articlethe expression pattern of STM does not change in as1 mutants
Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis
Visit the PubMed ArticleAfter germination, AS1 was detected in leaf founder cells from the time of primordium initiation
Byrne ME, Barley R, Curtis M, Arroyo JM, Dunham M, Hudson A, Martienssen RA - Asymmetric leaves1 mediates leaf patterning and stem cell function in Arabidopsis
Visit the PubMed Articleexpression of ATβ-Amy ... in plants grown on media lacking sugars did not differ between 35S::ATHB13 plants and wild type
Hanson J, Johannesson H, Engström P - Sugar-dependent alterations in cotyledon and leaf development in transgenic plants expressing the HDZhdip gene ATHB13
Visit the PubMed Articleexpression of ATβ-Amy ... was induced to higher levels in 35S::ATHB13 plants than in the wild type
Hanson J, Johannesson H, Engström P - Sugar-dependent alterations in cotyledon and leaf development in transgenic plants expressing the HDZhdip gene ATHB13
Visit the PubMed Articleexpression of ... VSP was induced to higher levels in 35S::ATHB13 plants than in the wild type
Hanson J, Johannesson H, Engström P - Sugar-dependent alterations in cotyledon and leaf development in transgenic plants expressing the HDZhdip gene ATHB13
Visit the PubMed ArticleCHS ... not affected by ATHB13 over-expression
Hanson J, Johannesson H, Engström P - Sugar-dependent alterations in cotyledon and leaf development in transgenic plants expressing the HDZhdip gene ATHB13
Visit the PubMed Articleexpression of ... VSP ... in plants grown on media lacking sugars did not differ between 35S::ATHB13 plants and wild type
Hanson J, Johannesson H, Engström P - Sugar-dependent alterations in cotyledon and leaf development in transgenic plants expressing the HDZhdip gene ATHB13
Visit the PubMed ArticleKNAT1 ... transcripts were detected in the peripheral zones ... in the wild type
Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves
Visit the PubMed Articlewild-type leaves did not accumulate any detectable STM transcripts
Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves
Visit the PubMed Articleas1-1 ... levels of KNAT1 ... transcripts ... were higher than in the wild type
Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves
Visit the PubMed ArticleKNAT1 transcripts ... as2-1 ... were detected ... not in the ... presumptive leaf initials
Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves
Visit the PubMed ArticleKNAT1 transcripts ... were detected ... not in the ... presumptive leaf initials ... in the wild type
Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves
Visit the PubMed ArticleLevels of STM transcripts in as2- 1 rosette leaves fluctuated
Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves
Visit the PubMed ArticleIn the rosette leaves of as1 ... levels of transcripts of the KNAT6 gene increased
Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves
Visit the PubMed ArticleKNAT1 transcripts ... were detected in the ... basal regions of leaf primordia ... in the wild type
Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves
Visit the PubMed ArticleKNAT1 transcripts ... as2-1 ... were detected ... not in the ... small leaf primordia
Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves
Visit the PubMed ArticleKNAT1 transcripts ... were detected ... not in the central zones ... in the wild type
Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves
Visit the PubMed Articleas2-1 ... levels of KNAT1 transcripts ... were higher than in the wild type
Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves
Visit the PubMed ArticleWhen primers for amplification of KNAT1 cDNA were used, products of PCR were detected in samples from all the leaves ... of ... as2 ... but not of wild-type plants
Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves
Visit the PubMed ArticleKNAT1 transcripts ... as2-1 ... were detected in the ... basal regions of leaf primordia
Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves
Visit the PubMed ArticleKNAT1 ... we occasionally detected weak but significant hybridization signals in some as2-1 leaf primordia and such signals were not detected in the wild type
Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves
Visit the PubMed ArticleNo significant increase in the accumulation of WUS transcripts was detected in as1-1 ... rosette leaves
Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves
Visit the PubMed ArticleTranscripts of the STM gene accumulated in the first and second rosette leaves of as1-1 plants
Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves
Visit the PubMed ArticleWhen primers for amplification of KNAT1 cDNA were used, products of PCR were detected in samples from all the leaves ... of as1 ... but not of wild-type plants
Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves
Visit the PubMed ArticleThe pattern of accumulation of KNAT1 transcripts in the as1-1 meristem was also similar to that in the wild type
Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves
Visit the PubMed ArticleNo significant increase in the accumulation of WUS transcripts was detected in ... as2-1 rosette leaves
Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves
Visit the PubMed ArticleKNAT1 ... we detected relatively strong hybridization signals at the bases of as2-1 leaf primordia
Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves
Visit the PubMed ArticleKNAT1 transcripts ... were detected ... not in the ... small leaf primordia ... in the wild type
Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves
Visit the PubMed ArticleWhen we used primers for KNAT2 cDNA, increased levels of products were detected in ... as2
Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves
Visit the PubMed ArticleWhen we used primers for KNAT2 cDNA, increased levels of products were detected in ... as1
Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves
Visit the PubMed ArticleKNAT6 ... in the wild type, transcripts of this gene accumulated in the shoot apices
Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves
Visit the PubMed ArticleIn situ hybridization showed that the pattern of accumulation of KNAT1 transcripts around vegetative meristems of as2-1 was similar to that of the wild type
Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves
Visit the PubMed ArticleKNAT1 ... as2-1 ... transcripts were detected in the peripheral zones
Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves
Visit the PubMed ArticleIn the rosette leaves of ... as2 ... levels of transcripts of the KNAT6 gene increased
Semiarti E, Ueno Y, Tsukaya H, Iwakawa H, Machida C, Machida Y - The ASYMMETRIC LEAVES2 gene of Arabidopsis thaliana regulates formation of a symmetric lamina, establishment of venation and repression of meristem-related homeobox genes in leaves
Visit the PubMed ArticleKAN ... soon thereafter becomes restricted to peripheral cells in a basal region of the embryo
Kerstetter RA, Bollman K, Taylor RA, Bomblies K, Poethig RS - KANADI regulates organ polarity in Arabidopsis
Visit the PubMed ArticleTranslational fusions between the KAN cDNA and ... b-glucuronidase (GUS ... KAN directed reporter gene expression to the nucleus
Kerstetter RA, Bollman K, Taylor RA, Bomblies K, Poethig RS - KANADI regulates organ polarity in Arabidopsis
Visit the PubMed ArticleKAN ... In heart-stage embryos, expression was observed both in the periphery of the presumptive hypocotyl
Kerstetter RA, Bollman K, Taylor RA, Bomblies K, Poethig RS - KANADI regulates organ polarity in Arabidopsis
Visit the PubMed ArticleKAN is weakly expressed throughout the early globular embryo
Kerstetter RA, Bollman K, Taylor RA, Bomblies K, Poethig RS - KANADI regulates organ polarity in Arabidopsis
Visit the PubMed ArticleDuring vegetative growth, KANmRNAwas observed ... not in older leaves
Kerstetter RA, Bollman K, Taylor RA, Bomblies K, Poethig RS - KANADI regulates organ polarity in Arabidopsis
Visit the PubMed ArticleDuring vegetative growth, KANmRNAwas observed on the abaxial side of very young leaf primordia
Kerstetter RA, Bollman K, Taylor RA, Bomblies K, Poethig RS - KANADI regulates organ polarity in Arabidopsis
Visit the PubMed ArticleKAN ... In heart-stage embryos, expression was observed ... on the abaxial side of cotyledon primordia
Kerstetter RA, Bollman K, Taylor RA, Bomblies K, Poethig RS - KANADI regulates organ polarity in Arabidopsis
Visit the PubMed ArticleTranslational fusions between the KAN cDNA and ... modified green fluorescent protein (mGFP5 ... KAN directed reporter gene expression to the nucleus
Kerstetter RA, Bollman K, Taylor RA, Bomblies K, Poethig RS - KANADI regulates organ polarity in Arabidopsis
Visit the PubMed ArticleThe highest levels of PHB RNA are in adaxial (or central) regions of the embryo
McConnell JR, Emery J, Eshed Y, Bao N, Bowman J, Barton MK - Role of PHABULOSA and PHAVOLUTA in determining radial patterning in shoots
Visit the PubMed ArticlePHB transcript is initially present throughout the presumptive cotyledons
McConnell JR, Emery J, Eshed Y, Bao N, Bowman J, Barton MK - Role of PHABULOSA and PHAVOLUTA in determining radial patterning in shoots
Visit the PubMed ArticlePHB expression in the meristem are missing in phb-1d mutants
McConnell JR, Emery J, Eshed Y, Bao N, Bowman J, Barton MK - Role of PHABULOSA and PHAVOLUTA in determining radial patterning in shoots
Visit the PubMed ArticleThe PHB transcript is preferentially expressed in the adaxial domain of the developing leaf
McConnell JR, Emery J, Eshed Y, Bao N, Bowman J, Barton MK - Role of PHABULOSA and PHAVOLUTA in determining radial patterning in shoots
Visit the PubMed ArticlePHB ... globular stage just before cotyledon outgrowth) is seen at high levels in their adaxial domains
McConnell JR, Emery J, Eshed Y, Bao N, Bowman J, Barton MK - Role of PHABULOSA and PHAVOLUTA in determining radial patterning in shoots
Visit the PubMed ArticleThus, in young primordia, the unit accumulating PHB transcript is the adaxial leaf domain and adjacent meristem cells
McConnell JR, Emery J, Eshed Y, Bao N, Bowman J, Barton MK - Role of PHABULOSA and PHAVOLUTA in determining radial patterning in shoots
Visit the PubMed ArticleTo visualize the deduced decrease in cell division, we analyzed a mitotic reporter (19, 20) in the gpa1 background. b-Glucuronidase (GUS) staining of both the apical meristems and basal cells of the first leaf was markedly reduced in gpa1 mutants compared with con- trols
Ullah H, Chen JG, Young JC, Im KH, Sussman MR, Jones AM - Modulation of cell proliferation by heterotrimeric G protein in Arabidopsis
Visit the PubMed ArticleGPA1 expression in actively dividing cells
Ullah H, Chen JG, Young JC, Im KH, Sussman MR, Jones AM - Modulation of cell proliferation by heterotrimeric G protein in Arabidopsis
Visit the PubMed ArticleThe levels of BAS1 transcripts were not detectable in dwf4
Choe S, Fujioka S, Noguchi T, Takatsuto S, Yoshida S, Feldmann KA - Overexpression of DWARF4 in the brassinosteroid biosynthetic pathway results in increased vegetative growth and seed yield in Arabidopsis
Visit the PubMed ArticleThe levels of BAS1 transcripts were ... decreased in bri1
Choe S, Fujioka S, Noguchi T, Takatsuto S, Yoshida S, Feldmann KA - Overexpression of DWARF4 in the brassinosteroid biosynthetic pathway results in increased vegetative growth and seed yield in Arabidopsis
Visit the PubMed ArticleDET2 ... transcript levels were increased in the ... bri1 mutant plants
Choe S, Fujioka S, Noguchi T, Takatsuto S, Yoshida S, Feldmann KA - Overexpression of DWARF4 in the brassinosteroid biosynthetic pathway results in increased vegetative growth and seed yield in Arabidopsis
Visit the PubMed ArticleCPD transcript levels were increased in the ... bri1 mutant plants
Choe S, Fujioka S, Noguchi T, Takatsuto S, Yoshida S, Feldmann KA - Overexpression of DWARF4 in the brassinosteroid biosynthetic pathway results in increased vegetative growth and seed yield in Arabidopsis
Visit the PubMed ArticleCPD transcript levels were increased in the dwf4 ... mutant plants
Choe S, Fujioka S, Noguchi T, Takatsuto S, Yoshida S, Feldmann KA - Overexpression of DWARF4 in the brassinosteroid biosynthetic pathway results in increased vegetative growth and seed yield in Arabidopsis
Visit the PubMed Articleoverexpresses the DWF4 gene ... The level of TCH4 was significantly increased in AOD4 compared to both wild-type and dwarf mutants
Choe S, Fujioka S, Noguchi T, Takatsuto S, Yoshida S, Feldmann KA - Overexpression of DWARF4 in the brassinosteroid biosynthetic pathway results in increased vegetative growth and seed yield in Arabidopsis
Visit the PubMed ArticleDET2 ... transcript levels were increased in the dwf4 ... mutant plants
Choe S, Fujioka S, Noguchi T, Takatsuto S, Yoshida S, Feldmann KA - Overexpression of DWARF4 in the brassinosteroid biosynthetic pathway results in increased vegetative growth and seed yield in Arabidopsis
Visit the PubMed Articleoverexpresses the DWF4 gene ... The levels of BAS1 transcripts were ... slightly increased in AOD4 relative to the wild type
Choe S, Fujioka S, Noguchi T, Takatsuto S, Yoshida S, Feldmann KA - Overexpression of DWARF4 in the brassinosteroid biosynthetic pathway results in increased vegetative growth and seed yield in Arabidopsis
Visit the PubMed ArticlemRNA of REV ... restricted to the adaxial domain as developing primordia
Eshed Y, Baum SF, Perea JV, Bowman JL - Establishment of polarity in lateral organs of plants
Visit the PubMed ArticleIn kan1 kan2 seedlings, initial FIL expression appears normal
Eshed Y, Baum SF, Perea JV, Bowman JL - Establishment of polarity in lateral organs of plants
Visit the PubMed ArticleREV ... initiates normally in kan1 kan2 leaf primordia
Eshed Y, Baum SF, Perea JV, Bowman JL - Establishment of polarity in lateral organs of plants
Visit the PubMed ArticlemRNA of ... PHV ... Later expression is confined to the provascular and vascular tissues of leaves
Eshed Y, Baum SF, Perea JV, Bowman JL - Establishment of polarity in lateral organs of plants
Visit the PubMed ArticlemRNA of ... PHV is localized to the SAM, throughout leaf primordia anlagen
Eshed Y, Baum SF, Perea JV, Bowman JL - Establishment of polarity in lateral organs of plants
Visit the PubMed ArticlemRNA of REV ... Later expression is confined to the provascular and vascular tissues of leaves
Eshed Y, Baum SF, Perea JV, Bowman JL - Establishment of polarity in lateral organs of plants
Visit the PubMed ArticleIn wild-type, mRNA of FIL is first detected throughout leaf primordia anlagen
Eshed Y, Baum SF, Perea JV, Bowman JL - Establishment of polarity in lateral organs of plants
Visit the PubMed ArticlemRNA of ... PHV is localized to the SAM
Eshed Y, Baum SF, Perea JV, Bowman JL - Establishment of polarity in lateral organs of plants
Visit the PubMed ArticlemRNA of REV ... is localized to the SAM
Eshed Y, Baum SF, Perea JV, Bowman JL - Establishment of polarity in lateral organs of plants
Visit the PubMed ArticleREV ... in kan1 kan2 leaf primordia ... confinement to the adaxial domain is delayed
Eshed Y, Baum SF, Perea JV, Bowman JL - Establishment of polarity in lateral organs of plants
Visit the PubMed ArticlePHV ... in kan1 kan2 leaf primordia ... confinement to the adaxial domain is delayed
Eshed Y, Baum SF, Perea JV, Bowman JL - Establishment of polarity in lateral organs of plants
Visit the PubMed ArticlemRNA of ... PHV is ... restricted to the adaxial domain as developing primordia
Eshed Y, Baum SF, Perea JV, Bowman JL - Establishment of polarity in lateral organs of plants
Visit the PubMed Articlekan1 kan2 seedlings ... FIL ... domain is reduced in size. In developing leaf primordia, FIL was not detected in more than 2-3 cell layers, even though these primordia have more cell layers than wild-type
Eshed Y, Baum SF, Perea JV, Bowman JL - Establishment of polarity in lateral organs of plants
Visit the PubMed ArticlePHV, initiates normally in kan1 kan2 leaf primordia
Eshed Y, Baum SF, Perea JV, Bowman JL - Establishment of polarity in lateral organs of plants
Visit the PubMed ArticlemRNA of REV ... is localized to the SAM, throughout leaf primordia anlagen
Eshed Y, Baum SF, Perea JV, Bowman JL - Establishment of polarity in lateral organs of plants
Visit the PubMed Articlekan1 kan2 ... PHV, levels of mRNA are higher
Eshed Y, Baum SF, Perea JV, Bowman JL - Establishment of polarity in lateral organs of plants
Visit the PubMed ArticleIn wild-type, mRNA of FIL ... becomes confined to the abaxial side of the leaf
Eshed Y, Baum SF, Perea JV, Bowman JL - Establishment of polarity in lateral organs of plants
Visit the PubMed ArticleExpression of ELK4 (AGB1) was found in all tissues tested
Lease KA, Wen J, Li J, Doke JT, Liscum E, Walker JC - A mutant Arabidopsis heterotrimeric G-protein beta subunit affects leaf, flower, and fruit development
Visit the PubMed Articleto determine the level of AtCAP1 in distinct Arabidopsis tissues. After RT-PCR, a specific 410-bp fragment was detected in ... cotyledons
Barrero RA, Umeda M, Yamamura S, Uchimiya H - Arabidopsis CAP regulates the actin cytoskeleton necessary for plant cell elongation and division
Visit the PubMed Articleto determine the level of AtCAP1 in distinct Arabidopsis tissues. After RT-PCR, a specific 410-bp fragment was detected in ... shoots
Barrero RA, Umeda M, Yamamura S, Uchimiya H - Arabidopsis CAP regulates the actin cytoskeleton necessary for plant cell elongation and division
Visit the PubMed Articleto determine the level of AtCAP1 in distinct Arabidopsis tissues. After RT-PCR, a specific 410-bp fragment was detected in ... leaves
Barrero RA, Umeda M, Yamamura S, Uchimiya H - Arabidopsis CAP regulates the actin cytoskeleton necessary for plant cell elongation and division
Visit the PubMed ArticleThe quantitative estimation of the amount of protein accumulation indicated that although the actin level was maintained constant in all plants, the AtCAP1 level increased as the amount of Dex was increased in transgenic plants (Figure 6B). S4 plants accumulated AtCAP1 protein at high levels, whereas S11 plants accumulated AtCAP1 at low levels
Barrero RA, Umeda M, Yamamura S, Uchimiya H - Arabidopsis CAP regulates the actin cytoskeleton necessary for plant cell elongation and division
Visit the PubMed ArticleWe obtained several transgenic lines possessing a chimeric AtCAP1 gene under a GAL4 promoter, which is recognized by the GVG transcription factor upon activation with dexamethasone (Dex) (Aoyama and Chua, 1997) (Figure 6A) . Three independent transgenic plants, S4, S9, and S11, each with a single copy of AtCAP1 (data not shown) and with different expression levels of the AtCAP1 protein (Figure 6B), were chosen for further analysis
Barrero RA, Umeda M, Yamamura S, Uchimiya H - Arabidopsis CAP regulates the actin cytoskeleton necessary for plant cell elongation and division
Visit the PubMed ArticleTo characterize the level of accumulation of the AtCAP1 protein in Arabidopsis tissues, total proteins from suspension-cultured cells, roots, stems, leaves, and flowers were extracted and subjected to protein gel blot analysis as described in Methods. The AtCAP1 antibody specifically recognized a 52-kD protein, which is the predicted molecular mass of the AtCAP1 protein. A detectable level of AtCAP1 protein was found in suspension-cultured cells and roots
Barrero RA, Umeda M, Yamamura S, Uchimiya H - Arabidopsis CAP regulates the actin cytoskeleton necessary for plant cell elongation and division
Visit the PubMed ArticleAS1 was expressed in all plant tissues examined: roots, stems, leaves, flowers and siliques, with preferential expression in young and immature plant tissues. In the developed rosette and cauline leaves, AS1 had very low expression
Sun Y, Zhou Q, Zhang W, Fu Y, Huang H - ASYMMETRIC LEAVES1, an Arabidopsis gene that is involved in the control of cell differentiation in leaves
Visit the PubMed ArticleAtCUL1 is expressed in dividing cells throughout the plant (data not shown) and is localized primarily to the nucleus
del Pozo JC, Dharmasiri S, Hellmann H, Walker L, Gray WM, Estelle M - AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 is required for auxin response
Visit the PubMed ArticleTo further study AXR1 gene expression, we placed the β-glucuronidase reporter gene (GUS) adjacent to the promoter region of the AXR1 gene and introduced this construct into Arabidopsis plants ... Staining also was observed in the ... hydathodes
del Pozo JC, Dharmasiri S, Hellmann H, Walker L, Gray WM, Estelle M - AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 is required for auxin response
Visit the PubMed ArticleECR1 expression was observed in ... shoot meristems
del Pozo JC, Dharmasiri S, Hellmann H, Walker L, Gray WM, Estelle M - AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 is required for auxin response
Visit the PubMed ArticleECR1 RNA was not detected in mature (nongrowing) tissues of the plant, such as in the stem distal to the apex or in fully expanded leaves
del Pozo JC, Dharmasiri S, Hellmann H, Walker L, Gray WM, Estelle M - AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 is required for auxin response
Visit the PubMed ArticleTo further study AXR1 gene expression, we placed the β-glucuronidase reporter gene (GUS) adjacent to the promoter region of the AXR1 gene and introduced this construct into Arabidopsis plants ... we observed GUS staining in vascular tissues in cotyledons
del Pozo JC, Dharmasiri S, Hellmann H, Walker L, Gray WM, Estelle M - AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 is required for auxin response
Visit the PubMed Articlewe examined the pattern of AXR1 accumulation during embryogenesis. High levels of AXR1 protein were found in the zygote and throughout embryogenesis (Figures 1M to 1P and data not shown). A similar distribution of AXR1 RNA was observed (data not shown)
del Pozo JC, Dharmasiri S, Hellmann H, Walker L, Gray WM, Estelle M - AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 is required for auxin response
Visit the PubMed ArticleAXR1 protein was found in the ... leaf primordium
del Pozo JC, Dharmasiri S, Hellmann H, Walker L, Gray WM, Estelle M - AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 is required for auxin response
Visit the PubMed ArticleTo further study AXR1 gene expression, we placed the β-glucuronidase reporter gene (GUS) adjacent to the promoter region of the AXR1 gene and introduced this construct into Arabidopsis plants ... Staining also was observed in the trichomes
del Pozo JC, Dharmasiri S, Hellmann H, Walker L, Gray WM, Estelle M - AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 is required for auxin response
Visit the PubMed ArticleTo further study AXR1 gene expression, we placed the β-glucuronidase reporter gene (GUS) adjacent to the promoter region of the AXR1 gene and introduced this construct into Arabidopsis plants ... we observed GUS staining in vascular tissues in ... mature leaves
del Pozo JC, Dharmasiri S, Hellmann H, Walker L, Gray WM, Estelle M - AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 is required for auxin response
Visit the PubMed ArticleAXR1 RNA accumulated ... in young leaves
del Pozo JC, Dharmasiri S, Hellmann H, Walker L, Gray WM, Estelle M - AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 is required for auxin response
Visit the PubMed ArticleAXR1 was found primarily in the nuclei of these cells
del Pozo JC, Dharmasiri S, Hellmann H, Walker L, Gray WM, Estelle M - AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 is required for auxin response
Visit the PubMed ArticleAXR1 protein was found in the shoot meristem
del Pozo JC, Dharmasiri S, Hellmann H, Walker L, Gray WM, Estelle M - AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 is required for auxin response
Visit the PubMed ArticleAXR1 RNA accumulated in the root and shoot meristems
del Pozo JC, Dharmasiri S, Hellmann H, Walker L, Gray WM, Estelle M - AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 is required for auxin response
Visit the PubMed ArticleECR1 expression was observed in ... embryo
del Pozo JC, Dharmasiri S, Hellmann H, Walker L, Gray WM, Estelle M - AXR1-ECR1-dependent conjugation of RUB1 to the Arabidopsis Cullin AtCUL1 is required for auxin response
Visit the PubMed ArticleAN gene was expressed ... in ... leaves
Kim GT, Shoda K, Tsuge T, Cho KH, Uchimiya H, Yokoyama R, Nishitani K, Tsukaya H - The ANGUSTIFOLIA gene of Arabidopsis, a plant CtBP gene, regulates leaf-cell expansion, the arrangement of cortical microtubules in leaf cells and expression of a gene involved in cell-wall formation
Visit the PubMed ArticleMERI5 (Medford et al., 1991), was among the genes that were specifically up- regulated in the an mutant ... We found thatMERI5 was expressed at a 3-fold higher level in the an-1 mutant than in the wild type, as indicated by the microarray analysis (3.3-fold higher by microarray analysis; 3.27-fold higher by RT±PCR analy- sis
Kim GT, Shoda K, Tsuge T, Cho KH, Uchimiya H, Yokoyama R, Nishitani K, Tsukaya H - The ANGUSTIFOLIA gene of Arabidopsis, a plant CtBP gene, regulates leaf-cell expansion, the arrangement of cortical microtubules in leaf cells and expression of a gene involved in cell-wall formation
Visit the PubMed ArticleAN gene was expressed ... in cotyledons
Kim GT, Shoda K, Tsuge T, Cho KH, Uchimiya H, Yokoyama R, Nishitani K, Tsukaya H - The ANGUSTIFOLIA gene of Arabidopsis, a plant CtBP gene, regulates leaf-cell expansion, the arrangement of cortical microtubules in leaf cells and expression of a gene involved in cell-wall formation
Visit the PubMed Articlean ... We found no difference in numbers of MTs ... by moni- toring expression of the TUB6::GFP fusion gene
Kim GT, Shoda K, Tsuge T, Cho KH, Uchimiya H, Yokoyama R, Nishitani K, Tsukaya H - The ANGUSTIFOLIA gene of Arabidopsis, a plant CtBP gene, regulates leaf-cell expansion, the arrangement of cortical microtubules in leaf cells and expression of a gene involved in cell-wall formation
Visit the PubMed ArticleAN gene to a gene for green fluorescent protein (sGFP::S65T) under the control of the constitutively expressed 35S RNA promoter of cauliflower mosaic virus (CaMV ... we detected green fluorescence within the nuclei of cells in stable Arabidopsis transformants
Kim GT, Shoda K, Tsuge T, Cho KH, Uchimiya H, Yokoyama R, Nishitani K, Tsukaya H - The ANGUSTIFOLIA gene of Arabidopsis, a plant CtBP gene, regulates leaf-cell expansion, the arrangement of cortical microtubules in leaf cells and expression of a gene involved in cell-wall formation
Visit the PubMed Articlean ... Analysis by RT±PCR on one of the endogenous genes for tubulin, TUB4, indicated that there were no significant differences among the levels of expression of the transcript of this gene
Kim GT, Shoda K, Tsuge T, Cho KH, Uchimiya H, Yokoyama R, Nishitani K, Tsukaya H - The ANGUSTIFOLIA gene of Arabidopsis, a plant CtBP gene, regulates leaf-cell expansion, the arrangement of cortical microtubules in leaf cells and expression of a gene involved in cell-wall formation
Visit the PubMed Articlerot3 ... We found no difference in numbers of MTs ... by moni- toring expression of the TUB6::GFP fusion gene
Kim GT, Shoda K, Tsuge T, Cho KH, Uchimiya H, Yokoyama R, Nishitani K, Tsukaya H - The ANGUSTIFOLIA gene of Arabidopsis, a plant CtBP gene, regulates leaf-cell expansion, the arrangement of cortical microtubules in leaf cells and expression of a gene involved in cell-wall formation
Visit the PubMed ArticleEXGT-A1 ... did not show such differences (not significantly different from the wild type; level of significance, 5%) when we compared an-1 and wild-type plants
Kim GT, Shoda K, Tsuge T, Cho KH, Uchimiya H, Yokoyama R, Nishitani K, Tsukaya H - The ANGUSTIFOLIA gene of Arabidopsis, a plant CtBP gene, regulates leaf-cell expansion, the arrangement of cortical microtubules in leaf cells and expression of a gene involved in cell-wall formation
Visit the PubMed Articlerot3 ... Analysis by RT±PCR on one of the endogenous genes for tubulin, TUB4, indicated that there were no significant differences among the levels of expression of the transcript of this gene also
Kim GT, Shoda K, Tsuge T, Cho KH, Uchimiya H, Yokoyama R, Nishitani K, Tsukaya H - The ANGUSTIFOLIA gene of Arabidopsis, a plant CtBP gene, regulates leaf-cell expansion, the arrangement of cortical microtubules in leaf cells and expression of a gene involved in cell-wall formation
Visit the PubMed ArticleEXGT-A2 ... did not show such differences (not significantly different from the wild type; level of significance, 5%) when we compared an-1 and wild-type plants
Kim GT, Shoda K, Tsuge T, Cho KH, Uchimiya H, Yokoyama R, Nishitani K, Tsukaya H - The ANGUSTIFOLIA gene of Arabidopsis, a plant CtBP gene, regulates leaf-cell expansion, the arrangement of cortical microtubules in leaf cells and expression of a gene involved in cell-wall formation
Visit the PubMed ArticleAN ... fusion construct with green fluorescent protein (GFP) under the control of the 35S promotor from the cauliflower mosaic virus ... AN±GFP fusion protein was found ... in the cytoplasm and the nucleus
Folkers U, Kirik V, Schöbinger U, Falk S, Krishnakumar S, Pollock MA, Oppenheimer DG, Day I, Reddy AS, Jürgens G, Hülskamp M - The cell morphogenesis gene ANGUSTIFOLIA encodes a CtBP/BARS-like protein and is involved in the control of the microtubule cytoskeleton
Visit the PubMed Articlewe found AN expression in all tissues analyzed, including roots, leaves, stems and flowers
Folkers U, Kirik V, Schöbinger U, Falk S, Krishnakumar S, Pollock MA, Oppenheimer DG, Day I, Reddy AS, Jürgens G, Hülskamp M - The cell morphogenesis gene ANGUSTIFOLIA encodes a CtBP/BARS-like protein and is involved in the control of the microtubule cytoskeleton
Visit the PubMed ArticleAN ... fusion construct with green fluorescent protein (GFP) under the control of the 35S promotor from the cauliflower mosaic virus ... AN±GFP fusion protein was found ubiquitously in the cytoplasm and the nucleus
Folkers U, Kirik V, Schöbinger U, Falk S, Krishnakumar S, Pollock MA, Oppenheimer DG, Day I, Reddy AS, Jürgens G, Hülskamp M - The cell morphogenesis gene ANGUSTIFOLIA encodes a CtBP/BARS-like protein and is involved in the control of the microtubule cytoskeleton
Visit the PubMed Articleweak expression of SDD1 mRNA was observed in the mesophyll cells of developing rosette leaves and leaf primordia and in all cell layers of the entire shoot apical meristem
Von Groll U, Berger D, Altmann T - The subtilisin-like serine protease SDD1 mediates cell-to-cell signaling during Arabidopsis stomatal development
Visit the PubMed ArticleIn the 35S-SDD1ct-GFP–overexpressing lines, green fluorescence corresponding to GFP was localized to the plasma membrane (Figures 5A to 5D) . Similar fluorescence patterns were observed when leaves were incubated for 1 to 12 h in a buffer at pH 7.0 in an attempt to neutralize the apoplast, because GFP is unstable at low pH (Haseloff et al., 1997) (Figures 5A and 5B). Plasmolysis with 1 M KNO3 confirmed the localization of the GFP fusion protein in association with the plasma membrane (Figure 5B). Because SDD1 has no predicted membrane-spanning domain, the 35S-SDD1ct-GFP fusion protein may be attached to the plasma membrane via additional factors
Von Groll U, Berger D, Altmann T - The subtilisin-like serine protease SDD1 mediates cell-to-cell signaling during Arabidopsis stomatal development
Visit the PubMed ArticleIn paradermal sections of the epidermis, these specialized cell types were identified as meristemoids/GMCs (Ms/GMCs) according to their characteristic shape, as described in Zhao and Sack (1999) (Figure 2D
Von Groll U, Berger D, Altmann T - The subtilisin-like serine protease SDD1 mediates cell-to-cell signaling during Arabidopsis stomatal development
Visit the PubMed Articleweak expression of SDD1 mRNA was observed in the mesophyll cells of developing rosette leaves and leaf primordia and in all cell layers of the entire shoot apical meristem
Von Groll U, Berger D, Altmann T - The subtilisin-like serine protease SDD1 mediates cell-to-cell signaling during Arabidopsis stomatal development
Visit the PubMed Articleweak expression of SDD1 mRNA was observed in the mesophyll cells of developing rosette leaves and leaf primordia and in all cell layers of the entire shoot apical meristem
Von Groll U, Berger D, Altmann T - The subtilisin-like serine protease SDD1 mediates cell-to-cell signaling during Arabidopsis stomatal development
Visit the PubMed ArticleIn paradermal sections of the epidermis, these specialized cell types were identified as meristemoids/GMCs (Ms/GMCs) according to their characteristic shape, as described in Zhao and Sack (1999) (Figure 2D
Von Groll U, Berger D, Altmann T - The subtilisin-like serine protease SDD1 mediates cell-to-cell signaling during Arabidopsis stomatal development
Visit the PubMed Articleno SDD1 mRNA was detected in mature GCs
Von Groll U, Berger D, Altmann T - The subtilisin-like serine protease SDD1 mediates cell-to-cell signaling during Arabidopsis stomatal development
Visit the PubMed ArticleThe results showed that SDD1 was expressed predominantly in specialized cell types in the epidermis of developing leaves and siliques
Von Groll U, Berger D, Altmann T - The subtilisin-like serine protease SDD1 mediates cell-to-cell signaling during Arabidopsis stomatal development
Visit the PubMed Articlein the Rbx1-depleted plants, we found a significant higher level of cyclin D3 protein (Fig. 9) whereas cyclin D3 mRNAs accumulated at a similar level than in the Dex non-induced or control plants (data not shown). However, this protein band is detected at ∼46 kDa, whereas cyclin D3 detected in roots is ∼55 kDa (47). Because the antibody was raised against the C-terminal peptide of cyclin D3 (47), the 46-kDa form of the protein must represent the loss of an N-terminal fragment or could be an alternative splice form or a modified form of the protein
Lechner E, Xie D, Grava S, Pigaglio E, Planchais S, Murray JA, Parmentier Y, Mutterer J, Dubreucq B, Shen WH, Genschik P - The AtRbx1 protein is part of plant SCF complexes, and its down-regulation causes severe growth and developmental defects
Visit the PubMed ArticleRNA blot analyses were performed to investigate AtRbx1 expression, and its mRNAs could be detected in all the plant organs analyzed
Lechner E, Xie D, Grava S, Pigaglio E, Planchais S, Murray JA, Parmentier Y, Mutterer J, Dubreucq B, Shen WH, Genschik P - The AtRbx1 protein is part of plant SCF complexes, and its down-regulation causes severe growth and developmental defects
Visit the PubMed ArticleTo check whether the reductions of AtRbx1 and, to a lesser extent, AtCul1 would affect the pattern of ubiquitin conjugates, the blot was probed with an anti-ubiquitin antibody that recognizes both free and conjugated ubiquitin. Indeed, we noted that several protein bands either disappeared or were strongly reduced in the Dex-induced dsRNA line. The level of free ubiquitin also was reduced in those plants. However, the pattern of most ubiquitin conjugates were still similar between the non-induced and the induced line
Lechner E, Xie D, Grava S, Pigaglio E, Planchais S, Murray JA, Parmentier Y, Mutterer J, Dubreucq B, Shen WH, Genschik P - The AtRbx1 protein is part of plant SCF complexes, and its down-regulation causes severe growth and developmental defects
Visit the PubMed ArticleTo understand the biochemical basis of the severe growth defects observed in some of the dsRNA lines, protein extracts from dsRNA-2 line and control line were blotted with different antibodies (Fig. 9). Interestingly, we noted that AtRbx1-depleted seedlings do not only show a strong reduction of AtRbx1 protein level, but also a decreased AtCul1 protein level. In fact, both Rub1-modified and unmodified AtCul1 protein bands were weaker
Lechner E, Xie D, Grava S, Pigaglio E, Planchais S, Murray JA, Parmentier Y, Mutterer J, Dubreucq B, Shen WH, Genschik P - The AtRbx1 protein is part of plant SCF complexes, and its down-regulation causes severe growth and developmental defects
Visit the PubMed Articlediffuse localization of RHL2 throughout the nucleus
Sugimoto-Shirasu K, Stacey NJ, Corsar J, Roberts K, McCann MC - DNA topoisomerase VI is essential for endoreduplication in Arabidopsis
Visit the PubMed ArticleBP was expressed in leaves of the ... yab3-2
Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis
Visit the PubMed Articlefil-8 yab3-2 plants, STM expression ... was restricted to the apical meristem
Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis
Visit the PubMed Articleexpression of STM in the leaves ... not in wild-type plants
Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis
Visit the PubMed ArticleIn a wild-type background, BP::GUS expression is detected in the meristematic zone
Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis
Visit the PubMed ArticleIn seedlings heterozygous for yab3-2 ... we observed GUS expression on either side of the leaf margin in the younger tissues of leaf blades
Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis
Visit the PubMed ArticleYAB3 ... In seedlings heterozygous for yab3-2, GUS expression was observed in the young leaves
Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis
Visit the PubMed ArticleYAB3 ... In seedlings heterozygous for yab3-2 ... as the leaf matured, expression was restricted to the abaxial tissues of leaves
Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis
Visit the PubMed Articlefil-8 yab3-2 plants, STM expression was excluded from leaf anlagen
Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis
Visit the PubMed ArticleKNAT2 ... enhanced level of expression was detected in the leaves of ... yab3-2
Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis
Visit the PubMed ArticleIn most of the fil-8 seedlings, BP::GUS was ... prominent in the leaf blade margins
Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis
Visit the PubMed ArticleIn wild-type plants, STM expression was excluded from leaf anlagen
Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis
Visit the PubMed ArticleAs lateral organs emerged from the apical meristem, the expression of BP::GUS was downregulated
Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis
Visit the PubMed ArticleIn some fil-8 seedlings, BP::GUS expression appeared to be restricted to the abaxial domains of the emerging leaves
Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis
Visit the PubMed ArticleBP was expressed in leaves of the fil-8
Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis
Visit the PubMed ArticleSTM leaf expression was detectable even in fil-8
Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis
Visit the PubMed ArticleSTM leaf expression was detectable even in ... yab3-2 single-mutant plants
Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis
Visit the PubMed ArticleWe detected low-level expression of KNAT2 in the leaves of wild-type plants
Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis
Visit the PubMed ArticleIn most of the fil-8 seedlings, BP::GUS was detectable in emerging leaves
Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis
Visit the PubMed Articlestm mutant plants using the YAB3::GUS ... we detected no differences in the YAB gene expression pattern in the cotyledons of stm mutants and wild-type plants
Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis
Visit the PubMed ArticleBP was expressed in leaves of ... fil-8 yab3-2 double mutants
Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis
Visit the PubMed ArticleKNAT2 ... enhanced level of expression was detected in the leaves ... fil-8 yab3-2
Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis
Visit the PubMed Articlefil-8 yab3-2 plants ... We observed the expression of STM in the leaves of double mutants
Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis
Visit the PubMed ArticleYAB3 expression also was more intense near the leaf margins (Figure 2B). Similar to the previous results with the fil-5 yab3-1 mutants (Siegfried et al., 1999
Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis
Visit the PubMed ArticleWe also tested, by RT-PCR, the levels of AS1 transcripts in fil yab3 leaves, and these appeared unchanged compared with those in wild-type leaves
Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis
Visit the PubMed ArticleKNAT2 ... enhanced level of expression was detected in the leaves of ... fil-8
Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis
Visit the PubMed ArticleIn wild-type plants, STM expression was ... being restricted to the apical meristem
Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis
Visit the PubMed ArticleBP was expressed in leaves ... not in wild-type plants
Kumaran MK, Bowman JL, Sundaresan V - YABBY polarity genes mediate the repression of KNOX homeobox genes in Arabidopsis
Visit the PubMed ArticleSWP ... expression was maintained in developing leaves
Autran D, Jonak C, Belcram K, Beemster GT, Kronenberger J, Grandjean O, Inzé D, Traas J - Cell numbers and leaf development in Arabidopsis: a functional analysis of the STRUWWELPETER gene
Visit the PubMed ArticleSWP ... A weak signal, sometimes just above the detection level, was also visible at the vegetative shoot apical meristem
Autran D, Jonak C, Belcram K, Beemster GT, Kronenberger J, Grandjean O, Inzé D, Traas J - Cell numbers and leaf development in Arabidopsis: a functional analysis of the STRUWWELPETER gene
Visit the PubMed ArticleDuring embryogenesis, SWP mRNA was detected in all cells ... cotyledonar stage
Autran D, Jonak C, Belcram K, Beemster GT, Kronenberger J, Grandjean O, Inzé D, Traas J - Cell numbers and leaf development in Arabidopsis: a functional analysis of the STRUWWELPETER gene
Visit the PubMed ArticleDuring embryogenesis, SWP mRNA was detected in all cells, from the early octant ... stage
Autran D, Jonak C, Belcram K, Beemster GT, Kronenberger J, Grandjean O, Inzé D, Traas J - Cell numbers and leaf development in Arabidopsis: a functional analysis of the STRUWWELPETER gene
Visit the PubMed Articleswp ... At the level of the young primordia, downregulation of STM ... was systematically observed
Autran D, Jonak C, Belcram K, Beemster GT, Kronenberger J, Grandjean O, Inzé D, Traas J - Cell numbers and leaf development in Arabidopsis: a functional analysis of the STRUWWELPETER gene
Visit the PubMed Articlein swp ... At the level of the young primordia ... upregulation of the primordium marker AINTEGUMENTA (ANT) was systematically observed
Autran D, Jonak C, Belcram K, Beemster GT, Kronenberger J, Grandjean O, Inzé D, Traas J - Cell numbers and leaf development in Arabidopsis: a functional analysis of the STRUWWELPETER gene
Visit the PubMed ArticleSWP was strongly expressed in young leaf primordia
Autran D, Jonak C, Belcram K, Beemster GT, Kronenberger J, Grandjean O, Inzé D, Traas J - Cell numbers and leaf development in Arabidopsis: a functional analysis of the STRUWWELPETER gene
Visit the PubMed ArticleKNAT6 ... expression ... in the cotyledons ... bop1-1 seedlings were significantly higher than those in wild- type seedlings
Ha CM, Kim GT, Kim BC, Jun JH, Soh MS, Ueno Y, Machida Y, Tsukaya H, Nam HG - The BLADE-ON-PETIOLE 1 gene controls leaf pattern formation through the modulation of meristematic activity in Arabidopsis
Visit the PubMed Articlein the petioles of cotyledons and rosette leaves ... KNAT1::GUS was misexpressed ... in the bop1- 1
Ha CM, Kim GT, Kim BC, Jun JH, Soh MS, Ueno Y, Machida Y, Tsukaya H, Nam HG - The BLADE-ON-PETIOLE 1 gene controls leaf pattern formation through the modulation of meristematic activity in Arabidopsis
Visit the PubMed ArticleExamination of the expression pattern in single leaves of bop1-1 showed that, unlike in wild-type leaves, KNAT1::GUS was detected in the petioles of ... rosette leaves
Ha CM, Kim GT, Kim BC, Jun JH, Soh MS, Ueno Y, Machida Y, Tsukaya H, Nam HG - The BLADE-ON-PETIOLE 1 gene controls leaf pattern formation through the modulation of meristematic activity in Arabidopsis
Visit the PubMed ArticleKNAT1 ... expression ... in the cotyledons ... of bop1-1 seedlings were significantly higher than those in wild- type seedlings
Ha CM, Kim GT, Kim BC, Jun JH, Soh MS, Ueno Y, Machida Y, Tsukaya H, Nam HG - The BLADE-ON-PETIOLE 1 gene controls leaf pattern formation through the modulation of meristematic activity in Arabidopsis
Visit the PubMed ArticleIn bop1-1 plants, KNAT1::GUS was expressed in a pattern similar to that in wild-type plants, but in more expanded regions
Ha CM, Kim GT, Kim BC, Jun JH, Soh MS, Ueno Y, Machida Y, Tsukaya H, Nam HG - The BLADE-ON-PETIOLE 1 gene controls leaf pattern formation through the modulation of meristematic activity in Arabidopsis
Visit the PubMed Articlebop1-1 ... STM transcription was not detectable in our experiment in the ... cotyledons
Ha CM, Kim GT, Kim BC, Jun JH, Soh MS, Ueno Y, Machida Y, Tsukaya H, Nam HG - The BLADE-ON-PETIOLE 1 gene controls leaf pattern formation through the modulation of meristematic activity in Arabidopsis
Visit the PubMed ArticleKNAT2 ... bop1-1 ... there were no notable differences between the wild-type and mutant SAM regions in the expression
Ha CM, Kim GT, Kim BC, Jun JH, Soh MS, Ueno Y, Machida Y, Tsukaya H, Nam HG - The BLADE-ON-PETIOLE 1 gene controls leaf pattern formation through the modulation of meristematic activity in Arabidopsis
Visit the PubMed ArticleKNAT1 ... bop1-1 ... there were no notable differences between the wild-type and mutant SAM regions in the expression
Ha CM, Kim GT, Kim BC, Jun JH, Soh MS, Ueno Y, Machida Y, Tsukaya H, Nam HG - The BLADE-ON-PETIOLE 1 gene controls leaf pattern formation through the modulation of meristematic activity in Arabidopsis
Visit the PubMed ArticleExamination of the expression pattern in single leaves of bop1-1 showed that, unlike in wild-type leaves, KNAT1::GUS was detected in the petioles of cotyledons
Ha CM, Kim GT, Kim BC, Jun JH, Soh MS, Ueno Y, Machida Y, Tsukaya H, Nam HG - The BLADE-ON-PETIOLE 1 gene controls leaf pattern formation through the modulation of meristematic activity in Arabidopsis
Visit the PubMed ArticleKNAT2 ... expression ... the cotyledons ... of bop1-1 seedlings were significantly higher than those in wild- type seedlings
Ha CM, Kim GT, Kim BC, Jun JH, Soh MS, Ueno Y, Machida Y, Tsukaya H, Nam HG - The BLADE-ON-PETIOLE 1 gene controls leaf pattern formation through the modulation of meristematic activity in Arabidopsis
Visit the PubMed ArticleKNAT6 ... expression ... in the ... rosette leaves of bop1-1 seedlings were significantly higher than those in wild- type seedlings
Ha CM, Kim GT, Kim BC, Jun JH, Soh MS, Ueno Y, Machida Y, Tsukaya H, Nam HG - The BLADE-ON-PETIOLE 1 gene controls leaf pattern formation through the modulation of meristematic activity in Arabidopsis
Visit the PubMed Articlebop1-1 ... KNAT2 that showed only a slightly increased expression in the mutant rosette leaf
Ha CM, Kim GT, Kim BC, Jun JH, Soh MS, Ueno Y, Machida Y, Tsukaya H, Nam HG - The BLADE-ON-PETIOLE 1 gene controls leaf pattern formation through the modulation of meristematic activity in Arabidopsis
Visit the PubMed ArticleKNAT1 ... expression ... in the ... rosette leaves of bop1-1 seedlings were significantly higher than those in wild- type seedlings
Ha CM, Kim GT, Kim BC, Jun JH, Soh MS, Ueno Y, Machida Y, Tsukaya H, Nam HG - The BLADE-ON-PETIOLE 1 gene controls leaf pattern formation through the modulation of meristematic activity in Arabidopsis
Visit the PubMed Articlebop1-1 ... STM transcription was not detectable in our experiment in the ... rosette leaves
Ha CM, Kim GT, Kim BC, Jun JH, Soh MS, Ueno Y, Machida Y, Tsukaya H, Nam HG - The BLADE-ON-PETIOLE 1 gene controls leaf pattern formation through the modulation of meristematic activity in Arabidopsis
Visit the PubMed ArticleKNAT6 ... bop1-1 ... there were no notable differences between the wild-type and mutant SAM regions in the expression
Ha CM, Kim GT, Kim BC, Jun JH, Soh MS, Ueno Y, Machida Y, Tsukaya H, Nam HG - The BLADE-ON-PETIOLE 1 gene controls leaf pattern formation through the modulation of meristematic activity in Arabidopsis
Visit the PubMed ArticleIn wild-type plants, high levels of PNH mRNA are observed in developing vascular tissue
Newman KL, Fernandez AG, Barton MK - Regulation of axis determinacy by the Arabidopsis PINHEAD gene
Visit the PubMed Articlein situ hybridization experiments were performed on PNH; FIL::PNH transgenic plants using a PNH antisense probe. In addition to the wild-type expression domain, transformants expressed PNH ... near the margins on the abaxial sides of true leaves at levels resembling those on the adaxial sides
Newman KL, Fernandez AG, Barton MK - Regulation of axis determinacy by the Arabidopsis PINHEAD gene
Visit the PubMed ArticleIn wild-type plants ... PNH mRNA are observed ... on the adaxial, but not abaxial, sides of lateral organ primordia
Newman KL, Fernandez AG, Barton MK - Regulation of axis determinacy by the Arabidopsis PINHEAD gene
Visit the PubMed Articlein pnh; FIL::PNH embryos ... Similarly, STM was expressed only in its normal domain at the early and middle stages of embryogenesis, but it was expressed in some cotyledon primordia by the bent-cotyledon stage (Figures 8C and 8D). Unlike CUC2 expression, STM expression in cotyledon primordia was detected only after they were morphologically distinguishable as double cotyledons
Newman KL, Fernandez AG, Barton MK - Regulation of axis determinacy by the Arabidopsis PINHEAD gene
Visit the PubMed ArticleEarly in development, the expression of CUC2 in pnh; FIL::PNH embryos was confined to the same region as in the wild type (Figure 8A) . However, by the late heart stage, when cotyledon primordia are easily distinguishable, expression of this gene also was evident at the distal tip of some cotyledon primordia (Figure 8B). At later stages of embryogenesis, staining was seen in a higher proportion of cotyledon primordia
Newman KL, Fernandez AG, Barton MK - Regulation of axis determinacy by the Arabidopsis PINHEAD gene
Visit the PubMed ArticleIn wild-type plants ... PNH mRNA are observed in ... low levels ... throughout the SAM
Newman KL, Fernandez AG, Barton MK - Regulation of axis determinacy by the Arabidopsis PINHEAD gene
Visit the PubMed Articlein situ hybridization experiments were performed on PNH; FIL::PNH transgenic plants using a PNH antisense probe. In addition to the wild-type expression domain, transformants expressed PNH on the abaxial sides of cotyledons
Newman KL, Fernandez AG, Barton MK - Regulation of axis determinacy by the Arabidopsis PINHEAD gene
Visit the PubMed Articleno change in CYCD2;1 transcript levels was observed, despite the morphological and cytological changes apparent in CYCD3;1 OE plants
Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3
Visit the PubMed ArticleLevels of Rb mRNA were increased in ... mature rosette leaves (by 3.5-fold ... in CYCD3;1 OE
Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3
Visit the PubMed ArticleTo determine whether the effects of CYCD3;1 on the G1/S transition also resulted in a modification of the spatial localization of cell division, we assessed the overall distribution of cell proliferation using a fusion of GUS to the promoter and destruction box of the mitotic cyclin CYCB1;1 (Colon-Carmona et al., 1999). The CYCB1-GUS fusion protein is present only in cells in G2/M and is destroyed rapidly when cells passed through mitosis, so GUS staining patterns indicate regions containing cells engaged in active cell division. CYCD3;1 OE seedlings carrying this construct stained for GUS over a wider region than wild-type control seedlings (Figure 4C
Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3
Visit the PubMed Articlelarge increase in Rb protein level also was detected in CYCD3;1 OE seedlings
Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3
Visit the PubMed ArticleAll E2F genes were upregulated in CYCD3;1 OE plants
Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3
Visit the PubMed ArticleCYCD3;1 mRNA levels ... were not detected with a gel-based assay in mature rosette leaves
Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3
Visit the PubMed ArticleWe found that CYCD3;1 mRNA ... is present at high levels in young developing leaves
Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3
Visit the PubMed ArticleCYCD3;1 OE ... SHOOTMERISTEMLESS ... mRNA levels ... were unchanged despite the dramatic morphological differences
Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3
Visit the PubMed ArticleWe found that CYCD3;1 mRNA is abundant ... particularly in peripheral meristem areas
Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3
Visit the PubMed ArticleLittle or no change in CYCD3;2 ... mRNA levels was observed in CYCD3;1 OE seedlings
Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3
Visit the PubMed ArticleLow CYCD3;1 mRNA levels were detected in wild-type seedlings
Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3
Visit the PubMed Articlewe found that mRNA levels of ANT were unaffected in the transgenic ... CYCD3;1 OE
Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3
Visit the PubMed ArticleAll E2F genes were upregulated in CYCD3;1 OE plants
Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3
Visit the PubMed ArticleWe found that CYCD3;1 mRNA is abundant in ... young primordia
Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3
Visit the PubMed ArticleWe found that CYCD3;1 mRNA ... In more mature developing leaves, a strong signal persists in adaxial cells
Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3
Visit the PubMed ArticleWe found that CYCD3;1 mRNA is abundant in the vegetative shoot meristem
Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3
Visit the PubMed ArticleCYCD3;1 OE ... PHABULOSA ... mRNA levels ... were unchanged despite the dramatic morphological differences
Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3
Visit the PubMed ArticleLittle or no change in ... CYCD3;3 mRNA levels was observed in CYCD3;1 OE seedlings
Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3
Visit the PubMed ArticleAll E2F genes were upregulated in CYCD3;1 OE plants
Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3
Visit the PubMed ArticleLevels of Rb mRNA were increased ... by 5.7-fold ... in CYCD3;1 OE seedlings
Dewitte W, Riou-Khamlichi C, Scofield S, Healy JM, Jacqmard A, Kilby NJ, Murray JA - Altered cell cycle distribution, hyperplasia, and inhibited differentiation in Arabidopsis caused by the D-type cyclin CYCD3
Visit the PubMed ArticlepPHAB-GUS ... were introgressed into drl1-2 ... marker lines displayed promoter activity in the dorsal part of the leaf primordium, including the vascular bundles
Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants
Visit the PubMed ArticlepDRL1-GUS ... transverse sections of 8- to 12-day-old SAMs ... section more apical to the SAM showed circular ... staining that coincided with the central zone
Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants
Visit the PubMed ArticlepDRL1-GUS ... GUS activity also was observed typically around the vascular bun- dles
Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants
Visit the PubMed ArticlepDRL1-GUS ... In young leaf primordia, GUS activity occurred as a linear area representing the basal part of the dorsal side
Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants
Visit the PubMed ArticlepDRL1-GUS ... In older leaf primordia, X-Gluc staining was apparent as a continuous blue linear area ... the mesophyll be- tween the vascular bundles
Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants
Visit the PubMed ArticlepDRL1-GUS ... In transverse serial sec- tions of expanding leaves, GUS activity was patchy: staining was seen in individual palisade and spongy mesophyll paren- chyma cells
Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants
Visit the PubMed Articleleaves ... mRNA of DRL1 was detected in every plant organ investigated in the wild type
Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants
Visit the PubMed ArticleIn the strong drl1-2 allele with severely reduced DRL1 transcript, a significant upregulation of the AN transcript (up to twofold) was seen
Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants
Visit the PubMed ArticlepFIL-GUS ... introgressed into drl1-2 ... marker lines displayed promoter activity ... in the abaxial part of the leaf primordia, excluding vascular bundles
Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants
Visit the PubMed ArticlepDRL1-GUS ... longitudinal sections through shoot and inflorescence apices, GUS activity was ... absent in the L3 layer
Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants
Visit the PubMed ArticlepDRL1-GUS ... transverse sections of 8- to 12-day-old SAMs (n = 6), staining was ring shaped, indicating GUS activity in the periph- eral zone of the SAM
Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants
Visit the PubMed ArticlepDRL1-GUS ... GUS activity was not observed in the mesophyll cells at the leaf tip
Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants
Visit the PubMed ArticlepREV(DLUTA)-GUS ... were introgressed into drl1-2 ... marker lines displayed promoter activity in the dorsal part of the leaf primordium, including the vascular bundles
Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants
Visit the PubMed ArticlepDRL1-GUS ... In longitudinal sections through shoot and inflorescence apices, GUS activity was most prominent in the L2 layer
Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants
Visit the PubMed ArticlepDRL1-GUS ... GUS activity was not observed ... at the ventral mesophyll of the midrib
Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants
Visit the PubMed ArticleThe pDRL1-GUS pattern coincided with the patchy pat- tern of expression of pcyc1at-GUS during leaf development
Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants
Visit the PubMed ArticlepDRL1-GUS ... Homogeneous GUS activity was detected in globular-, heart-, and torpedo-stage embryos
Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants
Visit the PubMed ArticlepYAB3-GUS ... introgressed into drl1-2 ... marker lines displayed promoter activity ... in the abaxial part of the leaf primordia, excluding vascular bundles
Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants
Visit the PubMed Articlecotyledons ... mRNA of DRL1 was detected in every plant organ investigated in the wild type
Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants
Visit the PubMed ArticlepDRL1-GUS ... In the leaf epidermis, GUS activity was restricted to the stomatal guard cells, which are generated by cell division from epidermal meristemoids after cell division has ceased in the epidermal pavement cells
Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants
Visit the PubMed ArticlepDRL1-GUS ... GUS activity was not observed ... at the margin of the distal part of the leaf lamina
Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants
Visit the PubMed ArticlepDRL1-GUS ... pattern was identi- cal to the pattern of the dorsal marker lines pPHAB-GUS and pREV-GUS in leaf primordia
Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants
Visit the PubMed ArticlepDRL1-GUS ... In longitudinal sections through shoot and inflorescence apices, GUS activity was ... less prominent in the L1 layer
Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants
Visit the PubMed Articleshoot apices ... mRNA of DRL1 was detected in every plant organ investigated in the wild type
Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants
Visit the PubMed ArticlepDRL1-GUS ... In older leaf primordia, X-Gluc staining was apparent as a continuous blue linear area, including vascular bundles
Nelissen H, Clarke JH, De Block M, De Block S, Vanderhaeghen R, Zielinski RE, Dyer T, Lust S, Inzé D, Van Lijsebettens M - DRL1, a homolog of the yeast TOT4/KTI12 protein, has a function in meristem activity and organ growth in plants
Visit the PubMed ArticleWe used RNA in situ hybridizations and a β-glucuronidase (GUS) reporter gene for expression analysis of DRN/ESR1. To construct the GUS reporter, the DRN/ESR1 coding region was replaced by the GUS open reading frame in a genomic clone carrying 4.8-kb DNA sequences upstream of the ATG and 1.5-kb DNA sequences downstream of the termination codon ... from the torpedo stage onward, transcripts were found exclusively in the L1 layer of the SAM
Kirch T, Simon R, Grünewald M, Werr W - The DORNROSCHEN/ENHANCER OF SHOOT REGENERATION1 gene of Arabidopsis acts in the control of meristem ccll fate and lateral organ development
Visit the PubMed ArticleWe used RNA in situ hybridizations and a β-glucuronidase (GUS) reporter gene for expression analysis of DRN/ESR1. To construct the GUS reporter, the DRN/ESR1 coding region was replaced by the GUS open reading frame in a genomic clone carrying 4.8-kb DNA sequences upstream of the ATG and 1.5-kb DNA sequences downstream of the termination codon ... When the seedling had germinated, expression was found in young leaf primordia
Kirch T, Simon R, Grünewald M, Werr W - The DORNROSCHEN/ENHANCER OF SHOOT REGENERATION1 gene of Arabidopsis acts in the control of meristem ccll fate and lateral organ development
Visit the PubMed ArticleWe used RNA in situ hybridizations and a β-glucuronidase (GUS) reporter gene for expression analysis of DRN/ESR1. To construct the GUS reporter, the DRN/ESR1 coding region was replaced by the GUS open reading frame in a genomic clone carrying 4.8-kb DNA sequences upstream of the ATG and 1.5-kb DNA sequences downstream of the termination codon ... expression was found ... in the two outer cell layers, L1 and L2, of the SAM
Kirch T, Simon R, Grünewald M, Werr W - The DORNROSCHEN/ENHANCER OF SHOOT REGENERATION1 gene of Arabidopsis acts in the control of meristem ccll fate and lateral organ development
Visit the PubMed ArticleWe used RNA in situ hybridizations and a β-glucuronidase (GUS) reporter gene for expression analysis of DRN/ESR1. To construct the GUS reporter, the DRN/ESR1 coding region was replaced by the GUS open reading frame in a genomic clone carrying 4.8-kb DNA sequences upstream of the ATG and 1.5-kb DNA sequences downstream of the termination codon ... At the early heart stage, transcripts accumulated at high levels in the emerging cotyledons and at lower levels at the position of the prospective SAM (Figure 3B). Expression in cotyledons was only transient
Kirch T, Simon R, Grünewald M, Werr W - The DORNROSCHEN/ENHANCER OF SHOOT REGENERATION1 gene of Arabidopsis acts in the control of meristem ccll fate and lateral organ development
Visit the PubMed ArticleWe used RNA in situ hybridizations and a β-glucuronidase (GUS) reporter gene for expression analysis of DRN/ESR1. To construct the GUS reporter, the DRN/ESR1 coding region was replaced by the GUS open reading frame in a genomic clone carrying 4.8-kb DNA sequences upstream of the ATG and 1.5-kb DNA sequences downstream of the termination codon ... expression was detected first in the proembryo at the four-cell stage (Figure 3A) and throughout the embryo at the globular stage
Kirch T, Simon R, Grünewald M, Werr W - The DORNROSCHEN/ENHANCER OF SHOOT REGENERATION1 gene of Arabidopsis acts in the control of meristem ccll fate and lateral organ development
Visit the PubMed Articlewe produced transgenic Arabidopsis plants expressing a HST-GUS fusion protein under the regulation of the CaMV 35S promoter. hst-1 ... In trichomes ... GUS staining was clearly localized in the vicinity of the nuclear membrane
Bollman KM, Aukerman MJ, Park MY, Hunter C, Berardini TZ, Poethig RS - HASTY, the Arabidopsis ortholog of exportin 5/MSN5, regulates phase change and morphogenesis
Visit the PubMed Articlewe produced transgenic Arabidopsis plants expressing a HST-GUS fusion protein under the regulation of the CaMV 35S promoter. hst-1 plants containing the transgene were phenotypically wild type ... GUS activity was concentrated in the nucleus ... in ... trichomes
Bollman KM, Aukerman MJ, Park MY, Hunter C, Berardini TZ, Poethig RS - HASTY, the Arabidopsis ortholog of exportin 5/MSN5, regulates phase change and morphogenesis
Visit the PubMed Articlewe produced transgenic Arabidopsis plants expressing a HST-GUS fusion protein under the regulation of the CaMV 35S promoter. hst-1 ... In trichomes ... GUS staining ... was always most intense on the apical side of the nucleus
Bollman KM, Aukerman MJ, Park MY, Hunter C, Berardini TZ, Poethig RS - HASTY, the Arabidopsis ortholog of exportin 5/MSN5, regulates phase change and morphogenesis
Visit the PubMed ArticleHST ... Hybridization of this cDNA to poly(A)-enriched RNA revealed a single 4 kb transcript in leaves
Bollman KM, Aukerman MJ, Park MY, Hunter C, Berardini TZ, Poethig RS - HASTY, the Arabidopsis ortholog of exportin 5/MSN5, regulates phase change and morphogenesis
Visit the PubMed Articlewe produced transgenic Arabidopsis plants expressing a HST-GUS fusion protein under the regulation of the CaMV 35S promoter. hst-1 plants containing the transgene were phenotypically wild type ... GUS activity was concentrated in the nucleus and the surrounding cytoplasm ... in ... trichomes
Bollman KM, Aukerman MJ, Park MY, Hunter C, Berardini TZ, Poethig RS - HASTY, the Arabidopsis ortholog of exportin 5/MSN5, regulates phase change and morphogenesis
Visit the PubMed Articlevery young primordia of the shoot apical meristem ... expression was considerable ... of ... IGS
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleDR5::GUS ... In the young plants examined ... cotyledons did not show any GUS expression
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleDR5::GUS ... Different patterns of reporter-gene expression were observed in the well-developed 9-week-old plants, characterized by late-formed enlarged leaves (typical of short-day conditions), which continuously showed distinct patterns with GUS activity in the lobes
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleIn later developmental stages, in 5- to 9-mm-long leaves of 9-week-old plants, the expression of ... ILL was again similar in the margin and in the lamina
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleA striking difference between the shoot and the root was found in the extent of DR5::GUS expression in their apices. In none of the 100 shoot tips studied could GUS activity be detected in the ... youngest leaves
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleDR5::GUS ... In advanced stages of primordium development ... GUS activity occurred (away from the leaf margin) at the tips of the freely ending veinlets, or at intermediate sites along these smallest blind veinlets
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleDR5::GUS ... In the young plants examined, the two lowest leaves were almost free of GUS activity
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleDR5::GUS ... Different patterns of reporter-gene expression were observed in the well-developed 9-week-old plants, characterized by late-formed enlarged leaves (typical of short-day conditions), which continuously showed distinct patterns with GUS activity ... but nearly no expression in the lamina
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleDR5::GUS ... To ensure that this pattern of GUS expression did not reflect inhibition of penetration or reaction of -glucuronide, incisions were made in 24 leaf primordia at various developmental stages. By exposing the mesophyll to the GUS-staining solution, no induction of GUS expression could be detected along the inner-leaf surfaces (Fig. 1e). This incision control confirmed that no GUS activity existed below the free-auxin-producing tip during this early stage of leaf growth
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleDR5::GUS ... in the upper rosette leaves with slightly serrate edges, the marginal GUS expression was mainly restricted to the lobes
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleDR5::GUS ... At higher microscopic magnification, GUS expression was also observed in some of the bundles (Fig. 2c), indicating auxin flow within these veins
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleDR5::GUS ... In more developed primordia, new sites of GUS activity appeared basipetally along the distal regions of the leaf margin
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleIn later developmental stages, in 5- to 9-mm-long leaves of 9-week-old plants, the expression of IGS ... was again similar in the margin and in the lamina
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed Articlein very young primordia of the shoot apical meristem ... CHS expression was considerable
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleDR5::GUS ... Large hydathodes develop in the leaf tip and in the lobes. During the early stages of their differentiation, a center of high free-auxin production could be detected as a concentrated dark-blue GUS stain in the lobe (Fig. 2b, see Fig. 7b-d). From this very active center, a downward gradual decrease in GUS activity was observed from the margin to the differentiating bundle, which is assumed to function as a sink for the auxin flow originating in the primordium tip or in a lobe (Fig. 2b). In more developed hydathodes, the differentiating vessel elements extended towards the lobe margin, usually with more vessel elements in the distal parts of the bundle near the margin (Fig. 2c). Elevated GUS expression persisted in the lobes after xylem maturation in the hydathode (Fig. 3a, b
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleInterestingly, chalcone synthase, the key enzyme for biosynthesis of flavonoids, which are natural inhibitors of basipetal IAA transport and of IAA-degrading peroxidase, was strongly and preferentially expressed in the margin
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleIn later developmental stages, in 5- to 9-mm-long leaves of 9-week-old plants, the expression of ... ILL was again similar in the margin and in the lamina
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleIn later developmental stages, in 5- to 9-mm-long leaves of 9-week-old plants, the expression of IGS ... was again similar in the margin and in the lamina
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleThe largest difference was found in the expression of PIN1, an essential component of the basipetally directed auxin carrier (G�lweiler et al. 1998), with strong expression in the hydathode-containing margin
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleDR5::GUS ... Gradients of decreasing GUS expression could be detected along the margins of early developing primordia, being stronger in the distal than in the proximal parts below
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleCHS expression was more pronounced now in the margin
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed Articleindole-3-glycerol phosphate synthase (IGS), regarded as a key enzyme of the tryptophan-independent IAA synthesis pathway (Normanly and Bartel 1999), was strongly expressed along the hydathode-containing margin
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleDR5::GUS ... Different patterns of reporter-gene expression were observed in the well-developed 9-week-old plants, characterized by late-formed enlarged leaves (typical of short-day conditions), which continuously showed distinct patterns with GUS activity in the ... central part of the midvein
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleDR5::GUS ... Elevated GUS activity also occurred at and above bundle junctions along the primary and secondary veins (1*) as well as along the tertiary veins
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleIAA-amino acid hydrolase (ILL), which catalyzes the hydrolysis of IAA-amino acid conjugates (Normanly and Bartel 1999), was well expressed in ... lamina
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleDR5::GUS ... In advanced stages of primordium development, GUS expression was detected in the mesophyll, in central regions of the lamina
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleThe expression of NIT in very young primordia of the shoot apical meristem (Fig. 4a) was clearly stronger than in leaflets of 9-week-old plants
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleThe largest difference was found in the expression of PIN1, an essential component of the basipetally directed auxin carrier ... which was nearly absent in the lamina
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleDR5::GUS ... In young leaf primordia, i.e., in the second or third leaf (counted from the shoot apex), relatively low GUS activity was detected in the tip
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleCHS expression was ... nearly absent in the lamina
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleDR5::GUS ... GUS activity was visible at the base of some of the trichomes (Fig. 2a, g). During leaf primordium development, the trichomes showing GUS expression appeared in different sites along the primordium, near to, or far away from the margins. Those developing along the margins showed stronger GUS activity than those in central regions of the lamina
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleDR5::GUS ... In advanced stages of primordium development, GUS expression was detected ... at the sites of small vein formation (usually tertiary veins)
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleNitrilase (NIT 1-3), a key enzyme in the tryptophan-dependent IAA synthesis pathway (Hillebrand et al. 1998; Normanly and Bartel 1999), was equally expressed in ... lamina
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleIn young leaf primordia 2-5 mm long (compare Fig. 1g, i), indole-3-glycerol phosphate synthase (IGS), regarded as a key enzyme of the tryptophan-independent IAA synthesis pathway (Normanly and Bartel 1999), was ... expressed ... strongly in the lamina
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleIAA-amino acid hydrolase (ILL), which catalyzes the hydrolysis of IAA-amino acid conjugates (Normanly and Bartel 1999), was well expressed in ... margin
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleDR5::GUS ... Different patterns of reporter-gene expression were observed in the well-developed 9-week-old plants, characterized by late-formed enlarged leaves (typical of short-day conditions), which continuously showed distinct patterns with GUS activity in the ... margin
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleDR5::GUS ... In the lowest rosette leaves, characterized by rounded blades, GUS activity had spread evenly downward from the tip
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleDR5::GUS ... Later on, the GUS activity boosted and appeared as a dark blue point in the tip (Fig. 1f, see Fig. 7b). At this stage, no GUS expression could be detected in the leaf tissue below the free-auxin-producing tip of the young primordium, either along the margins, or in the lamina
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleInterestingly, chalcone synthase, the key enzyme for biosynthesis of flavonoids, which are natural inhibitors of basipetal IAA transport and of IAA-degrading peroxidase ... was ... weakly expressed in the lamina of the primordia
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleIn the youngest shoot region, a typical strong DR5::GUS expression was first detected in the stipules
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed Articlein very young primordia of the shoot apical meristem ... expression was considerable ... of ... PIN
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleA striking difference between the shoot and the root was found in the extent of DR5::GUS expression in their apices. In none of the 100 shoot tips studied could GUS activity be detected in the shoot promeristem
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed Articlein very young primordia of the shoot apical meristem ... expression was considerable ... of ILL
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed ArticleNitrilase (NIT 1-3), a key enzyme in the tryptophan-dependent IAA synthesis pathway (Hillebrand et al. 1998; Normanly and Bartel 1999), was equally expressed in ... margin
Aloni R, Schwalm K, Langhans M, Ullrich CI - Gradual shifts in sites of free-auxin production during leaf-primordium development and their role in vascular differentiation and leaf morphogenesis in Arabidopsis
Visit the PubMed Articlein-frame fusion between RCE1 and the β-glucuronidase (GUS) gene ... In leaves, particularly strong staining was observed in the ... trichomes
Dharmasiri S, Dharmasiri N, Hellmann H, Estelle M - The RUB/Nedd8 conjugation pathway is required for early development in Arabidopsis
Visit the PubMed Articlewe examined the status of CUL1 in wild-type and rce1-1 plants by immunoblotting. Figure 3A shows that the level of RUB–CUL1 in the mutant is significantly reduced compared with Ler plants
Dharmasiri S, Dharmasiri N, Hellmann H, Estelle M - The RUB/Nedd8 conjugation pathway is required for early development in Arabidopsis
Visit the PubMed Articlein-frame fusion between RCE1 and the β-glucuronidase (GUS) gene ... intense staining was observed at the shoot apex
Dharmasiri S, Dharmasiri N, Hellmann H, Estelle M - The RUB/Nedd8 conjugation pathway is required for early development in Arabidopsis
Visit the PubMed Articlein-frame fusion between RCE1 and the β-glucuronidase (GUS) gene ... In leaves, particularly strong staining was observed in the veins
Dharmasiri S, Dharmasiri N, Hellmann H, Estelle M - The RUB/Nedd8 conjugation pathway is required for early development in Arabidopsis
Visit the PubMed ArticleNULL
Dharmasiri S, Dharmasiri N, Hellmann H, Estelle M - The RUB/Nedd8 conjugation pathway is required for early development in Arabidopsis
Visit the PubMed ArticleTo investigate the pattern of RCE1 expression, we first examined expression in various plant tissues by RNA blotting. As indicated in Figure 1A, RCE1 RNA accumulates to substantial levels in all plant organs examined
Dharmasiri S, Dharmasiri N, Hellmann H, Estelle M - The RUB/Nedd8 conjugation pathway is required for early development in Arabidopsis
Visit the PubMed Articlejaw-D ... TCP gene with strong similarity to CIN was also downregulated
Palatnik JF, Allen E, Wu X, Schommer C, Schwab R, Carrington JC, Weigel D - Control of leaf morphogenesis by microRNAs
Visit the PubMed Articlejaw-D ... TCP gene with strong similarity to CIN was also downregulated
Palatnik JF, Allen E, Wu X, Schommer C, Schwab R, Carrington JC, Weigel D - Control of leaf morphogenesis by microRNAs
Visit the PubMed ArticleDuring the torpedo stage, TCP4 RNA becomes localized to subepidermal regions of the cotyledons, with highest levels near the tip (e–g). h, A similar pattern as in cotyledons is seen in young leaves of a wild-type seedling
Palatnik JF, Allen E, Wu X, Schommer C, Schwab R, Carrington JC, Weigel D - Control of leaf morphogenesis by microRNAs
Visit the PubMed ArticleTCP4 RNA becomes gradually restricted to emerging cotyledons during the heart stage
Palatnik JF, Allen E, Wu X, Schommer C, Schwab R, Carrington JC, Weigel D - Control of leaf morphogenesis by microRNAs
Visit the PubMed ArticleTCP4 mRNA in wild-type embryos ... initially uniform pattern during the globular stage
Palatnik JF, Allen E, Wu X, Schommer C, Schwab R, Carrington JC, Weigel D - Control of leaf morphogenesis by microRNAs
Visit the PubMed Articlejaw-D ... TCP gene with strong similarity to CIN was also downregulated
Palatnik JF, Allen E, Wu X, Schommer C, Schwab R, Carrington JC, Weigel D - Control of leaf morphogenesis by microRNAs
Visit the PubMed ArticleUsing RT–qPCR, we found that the MIR-JAW precursor was more abundant in the shoot apex, inflorescence and siliques
Palatnik JF, Allen E, Wu X, Schommer C, Schwab R, Carrington JC, Weigel D - Control of leaf morphogenesis by microRNAs
Visit the PubMed Articlejaw-D ... TCP gene with strong similarity to CIN was also downregulated
Palatnik JF, Allen E, Wu X, Schommer C, Schwab R, Carrington JC, Weigel D - Control of leaf morphogenesis by microRNAs
Visit the PubMed Articlejaw-D ... TCP gene with strong similarity to CIN was also downregulated
Palatnik JF, Allen E, Wu X, Schommer C, Schwab R, Carrington JC, Weigel D - Control of leaf morphogenesis by microRNAs
Visit the PubMed ArticleUsing RT–qPCR, we found that the MIR-JAW precursor was ... elevated in jaw-D mutants (Fig. 3b), mimicking the differential accumulation of the processed miRNA in different tissues and in jaw-D
Palatnik JF, Allen E, Wu X, Schommer C, Schwab R, Carrington JC, Weigel D - Control of leaf morphogenesis by microRNAs
Visit the PubMed ArticleDuring the torpedo stage, TCP4 RNA becomes localized to subepidermal regions of the cotyledons, with highest levels near the tip
Palatnik JF, Allen E, Wu X, Schommer C, Schwab R, Carrington JC, Weigel D - Control of leaf morphogenesis by microRNAs
Visit the PubMed Articleplants expressing an ARGOS– β-glucuronidase(GUS) ... GUS expression was detected in cotyledon vascular bundles
Hu Y, Xie Q, Chua NH - The Arabidopsis auxin-inducible gene ARGOS controls lateral organ size
Visit the PubMed ArticleARGOS induction by NAA still was observed in ... aux1-7
Hu Y, Xie Q, Chua NH - The Arabidopsis auxin-inducible gene ARGOS controls lateral organ size
Visit the PubMed ArticleARGOS was expressed at low levels in ... young rosette leaves
Hu Y, Xie Q, Chua NH - The Arabidopsis auxin-inducible gene ARGOS controls lateral organ size
Visit the PubMed ArticleARGOS induction by NAA still was observed in axr2
Hu Y, Xie Q, Chua NH - The Arabidopsis auxin-inducible gene ARGOS controls lateral organ size
Visit the PubMed Articlede- creased levels of ARGOS expression were observed in ... axr1-12 ... and the induction of ARGOS by NAA was ... abolished completely in axr1- 12
Hu Y, Xie Q, Chua NH - The Arabidopsis auxin-inducible gene ARGOS controls lateral organ size
Visit the PubMed ArticleARGOS induction by NAA still was observed in ... tir1-3
Hu Y, Xie Q, Chua NH - The Arabidopsis auxin-inducible gene ARGOS controls lateral organ size
Visit the PubMed Articleplants expressing an ARGOS– β-glucuronidase(GUS) ... 12-day-old seedlings, GUS staining was observed ... juvenile leaf blades
Hu Y, Xie Q, Chua NH - The Arabidopsis auxin-inducible gene ARGOS controls lateral organ size
Visit the PubMed Articlewe investigated the cellular localization of ARGOS in onion epi- dermal cells using 35S-ARGOS-GFP and 35S-GFP-ARGOS fusion genes ... ARGOS is distributed in the nucleus
Hu Y, Xie Q, Chua NH - The Arabidopsis auxin-inducible gene ARGOS controls lateral organ size
Visit the PubMed ArticleARGOS induction by NAA still was observed in ... iaa28
Hu Y, Xie Q, Chua NH - The Arabidopsis auxin-inducible gene ARGOS controls lateral organ size
Visit the PubMed Articlewe investigated the cellular localization of ARGOS in onion epi- dermal cells using 35S-ARGOS-GFP and 35S-GFP-ARGOS fusion genes ... ARGOS is distributed in the ... cytosol
Hu Y, Xie Q, Chua NH - The Arabidopsis auxin-inducible gene ARGOS controls lateral organ size
Visit the PubMed Articlede- creased levels of ARGOS expression were observed in axr1-3 ... and the induction of ARGOS by NAA was attenu- ated in the weak allele axr1-3
Hu Y, Xie Q, Chua NH - The Arabidopsis auxin-inducible gene ARGOS controls lateral organ size
Visit the PubMed Articleplants expressing an ARGOS– β-glucuronidase(GUS) ... In 12-day-old seedlings, GUS staining was observed ... juvenile leaf ... petioles
Hu Y, Xie Q, Chua NH - The Arabidopsis auxin-inducible gene ARGOS controls lateral organ size
Visit the PubMed ArticleANT expression persisted in rosette leaves of 40-day-old 35S-ARGOS plants
Hu Y, Xie Q, Chua NH - The Arabidopsis auxin-inducible gene ARGOS controls lateral organ size
Visit the PubMed Articleplants expressing an ARGOS– β-glucuronidase(GUS) ... In 12-day-old seedlings, GUS staining was observed clearly in leaf primordia
Hu Y, Xie Q, Chua NH - The Arabidopsis auxin-inducible gene ARGOS controls lateral organ size
Visit the PubMed ArticleARGOS ... was almost undetectable in mature leaves
Hu Y, Xie Q, Chua NH - The Arabidopsis auxin-inducible gene ARGOS controls lateral organ size
Visit the PubMed Articleprolonged expression of CycD3;1 also was detected in 35S-ARGOS leaves
Hu Y, Xie Q, Chua NH - The Arabidopsis auxin-inducible gene ARGOS controls lateral organ size
Visit the PubMed ArticleOne gene, which is identical to the putative gene At3g59900 in the Arabidopsis database, was found to be highly induced by NAA (data not shown). We designated this gene ARGOS (for Auxin-Regulated Gene involved in Organ Size; see below). RNA gel blot analysis confirmed the auxin induction of ARGOS in both roots and aerial parts (Figure 1B
Hu Y, Xie Q, Chua NH - The Arabidopsis auxin-inducible gene ARGOS controls lateral organ size
Visit the PubMed ArticleAtGRF7 ... predominantly expressed in shoot tips and flowers
Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis
Visit the PubMed ArticleAtGRF1 through AtGRF6 ... expression was low in mature stems and leaves
Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis
Visit the PubMed ArticleLeaf tissues were stained ... over the whole leaf area in ... AtGRF2::GUS
Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis
Visit the PubMed ArticleAtGRF1 through AtGRF6 genes were strongly expressed in roots, upper stems, and shoot tips containing the shoot apical meristem (SAM) and flower buds, as well as in mature flowers
Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis
Visit the PubMed ArticleThe SAM tissues were also stained in seedlings and at the reproductive stage of AtGRF3::GUS plants
Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis
Visit the PubMed ArticleAtGRF1 through AtGRF6 ... expression was low in mature stems and leaves
Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis
Visit the PubMed ArticleAtGRF1 through AtGRF6 genes were strongly expressed in roots, upper stems, and shoot tips containing the shoot apical meristem (SAM) and flower buds, as well as in mature flowers
Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis
Visit the PubMed ArticleAtGRF1 through AtGRF6 genes were strongly expressed in roots, upper stems, and shoot tips containing the shoot apical meristem (SAM) and flower buds, as well as in mature flowers
Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis
Visit the PubMed ArticleAtGRF1 through AtGRF6 genes were strongly expressed in roots, upper stems, and shoot tips containing the shoot apical meristem (SAM) and flower buds, as well as in mature flowers
Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis
Visit the PubMed ArticleAtGRF1 through AtGRF6 ... expression was low in mature stems and leaves
Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis
Visit the PubMed ArticleAtGRF1 through AtGRF6 ... expression was low in mature stems and leaves
Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis
Visit the PubMed ArticleAtGRF1 through AtGRF6 genes were strongly expressed in roots, upper stems, and shoot tips containing the shoot apical meristem (SAM) and flower buds, as well as in mature flowers
Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis
Visit the PubMed ArticleThe transcript of AtGRF9 was detected in the SAM and flower buds only after a prolonged exposure of the PhosphoImager screen (data not shown
Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis
Visit the PubMed ArticleStaining in leaves of AtGRF3::GUS plants was restricted to the very early stage of emerging leaves (Figure 3l), after which no staining in leaves was visible anymore (data not shown), reflecting the low transcript level of AtGRF3 in leaf tissues on an RNA gel blot
Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis
Visit the PubMed ArticleAtGRF1 through AtGRF6 genes were strongly expressed in roots, upper stems, and shoot tips containing the shoot apical meristem (SAM) and flower buds, as well as in mature flowers
Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis
Visit the PubMed ArticleAtGRF1 through AtGRF6 ... expression was low in mature stems and leaves
Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis
Visit the PubMed ArticleAtGRF8 ... predominantly expressed in shoot tips and flowers
Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis
Visit the PubMed ArticleLeaf tissues were stained along the veins ... in AtGRF1::GUS
Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis
Visit the PubMed ArticleAtGRF1 through AtGRF6 ... expression was low in mature stems and leaves
Kim JH, Choi D, Kende H - The AtGRF family of putative transcription factors is involved in leaf and cotyledon growth in Arabidopsis
Visit the PubMed Articleas1 35S:AS2 ... We detected no change in BP transcript levels in these plants
Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning
Visit the PubMed ArticleTranscript levels of ... YAB3 ... were reduced in 35S:AS2 shoots
Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning
Visit the PubMed ArticleAS2 transcript levels were increased slightly in ... fil-8 yab3-2 double mutants
Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning
Visit the PubMed ArticlePHB in as2-1 mutants. No significant differences in the transcript levels
Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning
Visit the PubMed ArticleAS2 transcript levels were increased slightly in ... phb-1d mutants
Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning
Visit the PubMed Article35S:AS2 ... Higher levels of WUS transcripts also were detected in class-IIB seedlings
Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning
Visit the PubMed Article35S:AS2 ... reduction in ... KNAT6 transcript levels was observed in ... transgenic seedlings
Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning
Visit the PubMed ArticleIn kan1 kan2 double mutants, AS2 transcripts were de- tected at wild-type levels
Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning
Visit the PubMed ArticleFIL ... in as2-1 mutants. No significant differences in the transcript levels
Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning
Visit the PubMed ArticleAS1 transcripts were detected in both petioles and blades of rosette leaves and appeared to be slightly more abundant in blade tissue, whereas AS2 transcripts were more abun- dant in petioles
Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning
Visit the PubMed Article35S:AS2 ... reduction in KNAT2 ... transcript levels was observed in ... transgenic seedlings
Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning
Visit the PubMed ArticleKAN2 ... in as2-1 mutants. No significant differences in the transcript levels
Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning
Visit the PubMed ArticleKAN2 transcripts were detectable in 35S:AS2 shoots, but at reduced levels compared with those in the wild type
Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning
Visit the PubMed ArticleKAN1 ... in as2-1 mutants. No significant differences in the transcript levels
Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning
Visit the PubMed ArticleAS1 transcripts were detected in all tissues tested
Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning
Visit the PubMed ArticleYAB3 ... in as2-1 mutants. No significant differences in the transcript levels
Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning
Visit the PubMed ArticleLOB transcripts were not detected in rosette leaf blades
Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning
Visit the PubMed ArticleTranscript levels of FIL ... were reduced in 35S:AS2 shoots
Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning
Visit the PubMed Article35S:AS2 ... reduction in ... BP transcript levels
Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning
Visit the PubMed ArticleKAN1 ... transcripts were detectable in 35S:AS2 shoots, but at reduced levels compared with those in the wild type
Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning
Visit the PubMed Article35S:AS2 plants ... KNAT2:GUS expression ... was reduced in some class- I seedlings and all class-II individuals
Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning
Visit the PubMed ArticleLOB transcripts ... were detected in petioles
Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning
Visit the PubMed Article35S:AS2 ... STM transcript levels ... were increased in class-IIB seedlings
Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning
Visit the PubMed ArticleAS1 transcripts were detected in both petioles and blades of rosette leaves and appeared to be slightly more abundant in blade tissue, whereas AS2 transcripts were more abun- dant in petioles
Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning
Visit the PubMed Articlethe majority of class-I 35S:AS2 plants retained high lev- els of KNAT2:GUS expression, similar to the parental line
Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning
Visit the PubMed ArticleAS2 transcripts ... were detected in cotyledons
Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning
Visit the PubMed ArticleAS2 transcripts were detected in rosette leaves, where they were more abundant in the petiole than in the blade
Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning
Visit the PubMed ArticleBP:GUS background, all 102 seedlings carrying the 35S:AS2 transgene showed a reduction in staining intensity relative to the wild type
Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning
Visit the PubMed Articletranscript levels of PHB, a gene that regulates adaxial identity, were increased in 35S:AS2 shoots of both classes, with the more severe class-IIB seedlings showing higher levels than class-I seedlings
Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning
Visit the PubMed ArticleAS2 transcripts were detected in most tissues tested
Lin WC, Shuai B, Springer PS - The Arabidopsis LATERAL ORGAN BOUNDARIES-domain gene ASYMMETRIC LEAVES2 functions in the repression of KNOX gene expression and in adaxial-abaxial patterning
Visit the PubMed ArticleAt 2 DAG, ARR5:GUS activity was completely absent in the shoot apical meristem (SAM) of 35S:AtCKX1 transgenic plants
Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity
Visit the PubMed ArticleAtCKX5:GUS ... was detected in the rib zone of the axillary shoot meristems
Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity
Visit the PubMed ArticleAtCKX4:GUS ... GUS staining was present in epidermal pavement cells
Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity
Visit the PubMed ArticleAtCKX6:GUS ... the AtCKX6 promoter was active during the maturation phase of stomatal guard cells but not in fully mature stomata in older leaves
Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity
Visit the PubMed Articleepidermal leaf cells of plants expressing high levels of AtCKX3-GFP accu- mulated fluorescence in the large central vacuole
Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity
Visit the PubMed ArticleAtCKX6:GUS expression ... was undetectable in fully expanded leaves
Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity
Visit the PubMed ArticleAtCKX1-GFP ... was not detected in vacuoles. However, AtCKX1-GFP fluorescence was detected in vacuoles of mainly smaller root cells, such as those of the vascular cylinder (Figure 10K). This finding suggests that vacuolar targeting of AtCKX1-GFP might be specific to certain cell types, or, more likely, that the AtCKX1-GFP expression level was lower and thus stained only smaller vacuoles. To- gether, these data support the conclusion that the final destina- tion of AtCKX1 and AtCKX3 is the vacuole
Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity
Visit the PubMed ArticleARR5:GUS activity was reduced strongly during various developmental stages of 35S:AtCKX1 plants
Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity
Visit the PubMed ArticleVery strong AtCKX4:GUS expression was detected in stipules
Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity
Visit the PubMed ArticleIn the shoot, AtCKX5:GUS expression was confined to the edges at the most basal part of the youngest leaves
Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity
Visit the PubMed ArticleAtCKX6:GUS expression was localized primarily to the vas- cular system of developing cotyledons
Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity
Visit the PubMed ArticleAtCKX2-GFP fluorescence to be distributed as a thin, more or less discontinuous line at the cell periphery and around nuclei (Figure 10D). In an optical section at the level of the cor- tex cytoplasm, the fluorescence appeared as a reticulate po- lygonal network often linked with punctuated structures (Fig- ure 10E). Based on published data, this distinct pattern can be identified as indicating localization to the ER
Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity
Visit the PubMed ArticleAtCKX2 promoter was detected in the shoot apex
Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity
Visit the PubMed ArticleHigh AtCKX4:GUS activity was localized to trichomes
Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity
Visit the PubMed ArticleAtCKX6:GUS expression was localized primarily to the vas- cular system of developing ... leaves
Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity
Visit the PubMed ArticleAtCKX3: GUS transformants, in which GUS expression was driven by a 1.46-kb promoter fragment, we occasionally detected very weak expression in the young shoot tissues 2 weeks after germination (data not shown
Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity
Visit the PubMed ArticleAtCKX1 promoter drove gene expression in the ... lateral shoot meristems
Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity
Visit the PubMed ArticleAtCKX6:GUS ... activity was strongest in the vasculature of young growing leaves. It gradually decreased basipetally in older leaves and was undetectable in fully expanded leaves
Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity
Visit the PubMed ArticleAtCKX2 promoter was detected ... in stipules
Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity
Visit the PubMed ArticleAtCKX3-GFP entered the ER lumen
Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity
Visit the PubMed ArticleAtCKX1 promoter drove gene expression in the shoot apex
Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity
Visit the PubMed Articlethe shoot, AtCKX5:GUS expression ... marking the developing leaf petiole
Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity
Visit the PubMed ArticleAtCKX1-GFP fusion marked the ER network in leaf epidermal cells
Werner T, Motyka V, Laucou V, Smets R, Van Onckelen H, Schmülling T - Cytokinin-deficient transgenic Arabidopsis plants show multiple developmental alterations indicating opposite functions of cytokinins in the regulation of shoot and root meristem activity
Visit the PubMed ArticleIn Northern blot experiments, ATHB16 mRNA was detected in all organs examined, the mRNA level being relatively high in rosette leaves, intermediate in roots, cauline leaves, inflorescences, and buds, but low in the stem and in siliques, as shown in Fig. 2A
Wang Y, Henriksson E, Söderman E, Henriksson KN, Sundberg E, Engström P - The Arabidopsis homeobox gene, ATHB16, regulates leaf development and the sensitivity to photoperiod in Arabidopsis
Visit the PubMed ArticleDHS transcript was detectable in RNA preparations from leaves at all stages, which indicates that expression of this gene is required throughout the course of leaf development and senescence (Figure 1B). Of particular interest, however, is the finding that DHS expression was strongly upregulated in the third pair of rosette leaves between days 7 and 14 after emergence (Figure 1B). This upregulation coincides with bolting, which marks initiation of the conversion from vegetative to reproductive growth. Between days 14 and 21 after emergence, DHS transcript levels in the third pair of rosette leaves declined again (Figure 1B). However, there was a further increase in transcript abundance through days 28 and 35 after emergence during the period when there is extensive catabolism of macromolecules within the dying cells and concurrent translocation of nutrients out of the senescing leaves into the developing seeds
Wang TW, Lu L, Zhang CG, Taylor C, Thompson JE - Pleiotropic effects of suppressing deoxyhypusine synthase expression in Arabidopsis thaliana
Visit the PubMed ArticleWe found no significant differences in the expression of the ... CycD3 ... the wild-type plants after auxin ... treatment
Pérez-Pérez JM, Ponce MR, Micol JL - The ULTRACURVATA2 gene of Arabidopsis encodes an FK506-binding protein involved in auxin and brassinosteroid signaling
Visit the PubMed ArticleWe found no significant differences in the expression of the ... CycD3 ... in the wild-type plants after ... BR treatment
Pérez-Pérez JM, Ponce MR, Micol JL - The ULTRACURVATA2 gene of Arabidopsis encodes an FK506-binding protein involved in auxin and brassinosteroid signaling
Visit the PubMed ArticleCPD ... down-regulated by brassinolide in the wild types studied, an effect that is abolished or extremely reduced by the ucu2 mutations
Pérez-Pérez JM, Ponce MR, Micol JL - The ULTRACURVATA2 gene of Arabidopsis encodes an FK506-binding protein involved in auxin and brassinosteroid signaling
Visit the PubMed ArticleROT3 ... down-regulated by brassinolide in the wild types studied, an effect that is abolished or extremely reduced by the ucu2 mutations
Pérez-Pérez JM, Ponce MR, Micol JL - The ULTRACURVATA2 gene of Arabidopsis encodes an FK506-binding protein involved in auxin and brassinosteroid signaling
Visit the PubMed ArticleWe found no significant differences in the expression of the NCED3 ... in the wild-type plants after ... BR treatment
Pérez-Pérez JM, Ponce MR, Micol JL - The ULTRACURVATA2 gene of Arabidopsis encodes an FK506-binding protein involved in auxin and brassinosteroid signaling
Visit the PubMed ArticleWe found no significant differences in the expression of the ... BEE1 ... in the wild-type plants after auxin ... treatment
Pérez-Pérez JM, Ponce MR, Micol JL - The ULTRACURVATA2 gene of Arabidopsis encodes an FK506-binding protein involved in auxin and brassinosteroid signaling
Visit the PubMed ArticleWe found no significant differences in the expression of the NCED3 ... in the wild-type plants after auxin ... treatment
Pérez-Pérez JM, Ponce MR, Micol JL - The ULTRACURVATA2 gene of Arabidopsis encodes an FK506-binding protein involved in auxin and brassinosteroid signaling
Visit the PubMed ArticleWe found no significant differences in the expression of the ... UCU1 ... in the wild-type plants after ... BR treatment
Pérez-Pérez JM, Ponce MR, Micol JL - The ULTRACURVATA2 gene of Arabidopsis encodes an FK506-binding protein involved in auxin and brassinosteroid signaling
Visit the PubMed ArticleWe found no significant differences in the expression of the ... UCU1 ... in the wild-type plants after auxin ... treatment
Pérez-Pérez JM, Ponce MR, Micol JL - The ULTRACURVATA2 gene of Arabidopsis encodes an FK506-binding protein involved in auxin and brassinosteroid signaling
Visit the PubMed ArticleWe found no significant differences in the expression of the ... BEE1 ... in the wild-type plants after ... BR treatment
Pérez-Pérez JM, Ponce MR, Micol JL - The ULTRACURVATA2 gene of Arabidopsis encodes an FK506-binding protein involved in auxin and brassinosteroid signaling
Visit the PubMed ArticleCPD ... found down-regulated by brassinolide in the wild types studied
Pérez-Pérez JM, Ponce MR, Micol JL - The ULTRACURVATA2 gene of Arabidopsis encodes an FK506-binding protein involved in auxin and brassinosteroid signaling
Visit the PubMed Articleexpression of ... CPD in the ucu1 mutant studied was higher than in the wild type in noninductive conditions
Pérez-Pérez JM, Ponce MR, Micol JL - The ULTRACURVATA2 gene of Arabidopsis encodes an FK506-binding protein involved in auxin and brassinosteroid signaling
Visit the PubMed Articleexpression of ROT3 ... in the ucu1 mutant studied was higher than in the wild type in noninductive conditions and did not change significantly by auxin or brassinolide treatment
Pérez-Pérez JM, Ponce MR, Micol JL - The ULTRACURVATA2 gene of Arabidopsis encodes an FK506-binding protein involved in auxin and brassinosteroid signaling
Visit the PubMed ArticleROT3 ... found down-regulated by brassinolide in the wild types studied
Pérez-Pérez JM, Ponce MR, Micol JL - The ULTRACURVATA2 gene of Arabidopsis encodes an FK506-binding protein involved in auxin and brassinosteroid signaling
Visit the PubMed Articlejag-3 ... JAG- VENUS fusion protein is located in the nucleus
Ohno CK, Reddy GV, Heisler MG, Meyerowitz EM - The Arabidopsis JAGGED gene encodes a zinc finger protein that promotes leaf tissue development
Visit the PubMed ArticleAs the cotyledon primordia continue to grow out during the heart stage, JAG mRNA expression is excluded from the tips of the cotyledon primordia but continues to be expressed in a band below this region of repressed expression
Ohno CK, Reddy GV, Heisler MG, Meyerowitz EM - The Arabidopsis JAGGED gene encodes a zinc finger protein that promotes leaf tissue development
Visit the PubMed ArticleIn the torpedo stage embryo, JAG expression continues to be absent at the tip of the cotyledon primordia (Fig. 6C). Cells in the epidermis of the cotyledon have a lower level of JAG transcripts than cells in the inner layers
Ohno CK, Reddy GV, Heisler MG, Meyerowitz EM - The Arabidopsis JAGGED gene encodes a zinc finger protein that promotes leaf tissue development
Visit the PubMed ArticleJAG transcripts ... are absent from the SAM
Ohno CK, Reddy GV, Heisler MG, Meyerowitz EM - The Arabidopsis JAGGED gene encodes a zinc finger protein that promotes leaf tissue development
Visit the PubMed ArticleJAG is also expressed in developing leaf primordia of the seedling at 3.5 days and/until 7 days after germination. It is initially expressed throughout the young primordium but later expression becomes excluded from the distal tip of the primordium (Fig. 6D-F). Reduced JAG expression is also detected at the proximal base of the leaf primordia
Ohno CK, Reddy GV, Heisler MG, Meyerowitz EM - The Arabidopsis JAGGED gene encodes a zinc finger protein that promotes leaf tissue development
Visit the PubMed ArticleJAG mRNA is first detected in late transition stage embryos in cells corresponding to the cotyledon primordia
Ohno CK, Reddy GV, Heisler MG, Meyerowitz EM - The Arabidopsis JAGGED gene encodes a zinc finger protein that promotes leaf tissue development
Visit the PubMed Articlejag-5D ... JAG RNA is also detected in the mutant in other tissues in which it is normally not expressed, such as the inflorescence meristem and ovules
Dinneny JR, Yadegari R, Fischer RL, Yanofsky MF, Weigel D - The role of JAGGED in shaping lateral organs
Visit the PubMed ArticleWe confirmed the predicted subcellular localization of JAG with a GFP fusion, which localizes to the nucleus
Dinneny JR, Yadegari R, Fischer RL, Yanofsky MF, Weigel D - The role of JAGGED in shaping lateral organs
Visit the PubMed ArticleJAG RNA accumulates in the initiating bracts of jag-5D heterozygotes (Fig. 6I), where it is maintained long after emergence of the bracts
Dinneny JR, Yadegari R, Fischer RL, Yanofsky MF, Weigel D - The role of JAGGED in shaping lateral organs
Visit the PubMed ArticleBy RT-PCR, JAG RNA is detected during vegetative and reproductive development in the shoot apex
Dinneny JR, Yadegari R, Fischer RL, Yanofsky MF, Weigel D - The role of JAGGED in shaping lateral organs
Visit the PubMed ArticleNo JAG expression was detected in the petioles
Dinneny JR, Yadegari R, Fischer RL, Yanofsky MF, Weigel D - The role of JAGGED in shaping lateral organs
Visit the PubMed ArticleJAG mRNA ... is absent from the shoot apical meristem
Dinneny JR, Yadegari R, Fischer RL, Yanofsky MF, Weigel D - The role of JAGGED in shaping lateral organs
Visit the PubMed ArticleJAG mRNA is detected in emerging leaf primordia
Dinneny JR, Yadegari R, Fischer RL, Yanofsky MF, Weigel D - The role of JAGGED in shaping lateral organs
Visit the PubMed Articlenot in ... leaves ... JGL mRNA
Dinneny JR, Yadegari R, Fischer RL, Yanofsky MF, Weigel D - The role of JAGGED in shaping lateral organs
Visit the PubMed ArticleSerial sections through leaf primordia revealed that JAG is predominantly expressed in the blade regions
Dinneny JR, Yadegari R, Fischer RL, Yanofsky MF, Weigel D - The role of JAGGED in shaping lateral organs
Visit the PubMed ArticleJAG mRNA is localized in the distal region of leaves
Dinneny JR, Yadegari R, Fischer RL, Yanofsky MF, Weigel D - The role of JAGGED in shaping lateral organs
Visit the PubMed ArticleBy RT-PCR, JAG RNA is ... not in ... leaves
Dinneny JR, Yadegari R, Fischer RL, Yanofsky MF, Weigel D - The role of JAGGED in shaping lateral organs
Visit the PubMed Articleis detected during vegetative and reproductive development in the shoot apex ... JGL mRNA
Dinneny JR, Yadegari R, Fischer RL, Yanofsky MF, Weigel D - The role of JAGGED in shaping lateral organs
Visit the PubMed Article1-h BR treatments resulted in increased EXO transcript levels in ... wild-type ... plants
Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes
Visit the PubMed ArticleThe KCS1 gene showed weaker expression in untreated dwf1-6 ... plants in comparison to untreated wild-type plants
Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes
Visit the PubMed ArticleBR-deficient dwf1-6 ... plants showed weaker EXO expression
Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes
Visit the PubMed ArticleExp5 ... induced upon 1-h BR treatments in ... dwf1-6 plants
Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes
Visit the PubMed ArticleReal-time RT-PCR analysis confirmed increased ... δ-TIP transcript levels in soil-grown 35S::EXO T2 generation plants
Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes
Visit the PubMed ArticleNorthern blot analysis confirmed elevated KCS1 ... mRNA levels in T1 generation 35S::EXO plants
Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes
Visit the PubMed ArticleIn agreement with our previous data, increased EXO mRNA levels were found in BR-treated plants (Table 1). Northern blot analysis confirmed the BR induction
Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes
Visit the PubMed ArticleCPD antisense plants showed weaker EXO expression
Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes
Visit the PubMed ArticleKCS1 ... decreased mRNA levels in T1 generation EXO-RNAi plants
Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes
Visit the PubMed Articleδ-TIP ... induced upon 1-h BR treatments in ... dwf1-6 plants
Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes
Visit the PubMed Article2,4-D (10 nM, 0.1 μM, and 1 μM; 1 h and 3 h) ... did not alter EXO transcript levels
Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes
Visit the PubMed ArticleAGP4 ... induced upon 1-h BR treatments in wild-type ... plants
Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes
Visit the PubMed ArticleGA (0.1 μM and 1 μM GA4/GA7; 1 h and 3 h) ... did not alter EXO transcript levels
Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes
Visit the PubMed ArticleNorthern blot analysis confirmed elevated ... Exp5 ... mRNA levels in T1 generation 35S::EXO plants
Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes
Visit the PubMed Articleδ-TIP ... induced upon 1-h BR treatments in wild-type ... plants
Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes
Visit the PubMed ArticleNorthern blot analysis confirmed elevated ... AGP4 mRNA levels in T1 generation 35S::EXO plants
Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes
Visit the PubMed ArticleKCS1 ... induced upon 1-h BR treatments in ... dwf1-6 plants
Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes
Visit the PubMed ArticleThe KCS1 gene showed weaker expression in untreated ... CPD antisense plants in comparison to untreated wild-type plants
Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes
Visit the PubMed ArticleAGP4 ... decreased mRNA levels in T1 generation EXO-RNAi plants
Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes
Visit the PubMed ArticleExp5 ... decreased mRNA levels in T1 generation EXO-RNAi plants
Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes
Visit the PubMed Article1-h BR treatments resulted in increased EXO transcript levels in ... dwf1-6 plants
Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes
Visit the PubMed ArticleKCS1 ... induced upon 1-h BR treatments in wild-type ... plants
Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes
Visit the PubMed ArticleAGP4 ... induced upon 1-h BR treatments in ... dwf1-6 plants
Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes
Visit the PubMed Articleethylene precursor ACC (0.1 μM, 1 μM; 1 h and 3 h) did not alter EXO transcript levels
Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes
Visit the PubMed ArticleExp5 ... induced upon 1-h BR treatments in wild-type ... plants
Coll-Garcia D, Mazuch J, Altmann T, Müssig C - EXORDIUM regulates brassinosteroid-responsive genes
Visit the PubMed Articletransgenic WIN1 overexpressors ... KCS1, which is predicted to encode a ketoacyl-CoA synthase involved in wax biosynthesis ... appeared to be up-regulated
Broun P, Poindexter P, Osborne E, Jiang CZ, Riechmann JL - WIN1, a transcriptional activator of epidermal wax accumulation in Arabidopsis
Visit the PubMed ArticleTo determine the effects of WIN1 on gene expression, Northern analysis was performed by using total RNA isolated from leaves of overexpressing lines ... CER1 presented the most striking change in gene expression: its transcript level was clearly correlated with the strength of WIN1 overexpression
Broun P, Poindexter P, Osborne E, Jiang CZ, Riechmann JL - WIN1, a transcriptional activator of epidermal wax accumulation in Arabidopsis
Visit the PubMed ArticleWIN1 is expressed at higher levels in flowers than in any other tissues. Expression was also detectable, although at lower levels, in stems, siliques, and rosette leaves
Broun P, Poindexter P, Osborne E, Jiang CZ, Riechmann JL - WIN1, a transcriptional activator of epidermal wax accumulation in Arabidopsis
Visit the PubMed Articletransgenic WIN1 overexpressors ... CER2, a presumed regulatory protein ... appeared to be up-regulated
Broun P, Poindexter P, Osborne E, Jiang CZ, Riechmann JL - WIN1, a transcriptional activator of epidermal wax accumulation in Arabidopsis
Visit the PubMed ArticleRNA gel blot analysis indicated that HLR mRNA accumulates in all the organs that we tested (flower bud, stem, leaf and root; data not shown), which is consistent with the pleiotropic phenotype of the hlr mutant. In particular, mRNA accumulated greatly in organs containing meristems, suggesting that the HLR gene works throughout the entire plant, especially in the meristematic regions
Ueda M, Matsui K, Ishiguro S, Sano R, Wada T, Paponov I, Palme K, Okada K - The HALTED ROOT gene encoding the 26S proteasome subunit RPT2a is essential for the maintenance of Arabidopsis meristems
Visit the PubMed ArticleWe then examined expression of the HLR gene in meristems by in situ RNA hybridization experiments ... In the shoot, the HLR gene was expressed uniformly in the SAM in both the vegetative and the reproductive phase
Ueda M, Matsui K, Ishiguro S, Sano R, Wada T, Paponov I, Palme K, Okada K - The HALTED ROOT gene encoding the 26S proteasome subunit RPT2a is essential for the maintenance of Arabidopsis meristems
Visit the PubMed Articlehlr mutants ... In 11-day-old mutant seedlings, the WUS expression domain was markedly enlarged, and was expanded to outer and/or inner cell layers (Fig. 5B). Furthermore, WUS mRNA accumulation was sometimes detected in some groups of cells at abnormal places
Ueda M, Matsui K, Ishiguro S, Sano R, Wada T, Paponov I, Palme K, Okada K - The HALTED ROOT gene encoding the 26S proteasome subunit RPT2a is essential for the maintenance of Arabidopsis meristems
Visit the PubMed ArticleNo signal was detected in the crl mutant
Asano T, Yoshioka Y, Kurei S, Sakamoto W, Machida Y; Sodmergen - A mutation of the CRUMPLED LEAF gene that encodes a protein localized in the outer envelope membrane of plastids affects the pattern of cell division, cell differentiation, and plastid division in Arabidopsis
Visit the PubMed ArticleThe CRL gene was ubiquitously expressed
Asano T, Yoshioka Y, Kurei S, Sakamoto W, Machida Y; Sodmergen - A mutation of the CRUMPLED LEAF gene that encodes a protein localized in the outer envelope membrane of plastids affects the pattern of cell division, cell differentiation, and plastid division in Arabidopsis
Visit the PubMed ArticleCRL protein is localized in the outer envelope membrane of chloroplasts
Asano T, Yoshioka Y, Kurei S, Sakamoto W, Machida Y; Sodmergen - A mutation of the CRUMPLED LEAF gene that encodes a protein localized in the outer envelope membrane of plastids affects the pattern of cell division, cell differentiation, and plastid division in Arabidopsis
Visit the PubMed ArticleThe ROT4 expression was highest in the shoot apices
Narita NN, Moore S, Horiguchi G, Kubo M, Demura T, Fukuda H, Goodrich J, Tsukaya H - Overexpression of a novel small peptide ROTUNDIFOLIA4 decreases cell proliferation and alters leaf shape in Arabidopsis thaliana
Visit the PubMed ArticleROT4 ... Expression in the mature cotyledons was extremely low
Narita NN, Moore S, Horiguchi G, Kubo M, Demura T, Fukuda H, Goodrich J, Tsukaya H - Overexpression of a novel small peptide ROTUNDIFOLIA4 decreases cell proliferation and alters leaf shape in Arabidopsis thaliana
Visit the PubMed ArticleWhen the ROT4 antisense probe was hybridized to rot4-1D materials, unlike wild type, a strong signal was consistently observed (Figure 4f�l). This agreed with the RT-PCR results, which also indicated higher expression in the rot4-1D mutant than in wild type. In seedlings, there was little expression in the shoot apical meristem (Figure 4f), whereas young leaf primordia showed expression throughout the lamina of young leaf primordia (Figure 4f,g). In cotyledons and older leaves, expression was restricted to vasculature (Figure 4f,g)
Narita NN, Moore S, Horiguchi G, Kubo M, Demura T, Fukuda H, Goodrich J, Tsukaya H - Overexpression of a novel small peptide ROTUNDIFOLIA4 decreases cell proliferation and alters leaf shape in Arabidopsis thaliana
Visit the PubMed ArticleTo determine the intracellular localization of the ROT4 protein, we examined young leaves of 35S::ROT4�GFP transgenic Arabidopsis plants using confocal microscopy ... ROT4�GFP fusion protein was localized to the plasma membrane
Narita NN, Moore S, Horiguchi G, Kubo M, Demura T, Fukuda H, Goodrich J, Tsukaya H - Overexpression of a novel small peptide ROTUNDIFOLIA4 decreases cell proliferation and alters leaf shape in Arabidopsis thaliana
Visit the PubMed ArticleThe ROT4 expression was ... somewhat reduced in the leaf primordia
Narita NN, Moore S, Horiguchi G, Kubo M, Demura T, Fukuda H, Goodrich J, Tsukaya H - Overexpression of a novel small peptide ROTUNDIFOLIA4 decreases cell proliferation and alters leaf shape in Arabidopsis thaliana
Visit the PubMed ArticleIn young seedlings, however, the expression of ROT4 was clearly detected in shoots
Narita NN, Moore S, Horiguchi G, Kubo M, Demura T, Fukuda H, Goodrich J, Tsukaya H - Overexpression of a novel small peptide ROTUNDIFOLIA4 decreases cell proliferation and alters leaf shape in Arabidopsis thaliana
Visit the PubMed ArticleROT4 is expressed in wild-type leaves during early developmental stages
Narita NN, Moore S, Horiguchi G, Kubo M, Demura T, Fukuda H, Goodrich J, Tsukaya H - Overexpression of a novel small peptide ROTUNDIFOLIA4 decreases cell proliferation and alters leaf shape in Arabidopsis thaliana
Visit the PubMed Articleaccumulation of ROT4 mRNA was not detected in mature rosette leaves
Narita NN, Moore S, Horiguchi G, Kubo M, Demura T, Fukuda H, Goodrich J, Tsukaya H - Overexpression of a novel small peptide ROTUNDIFOLIA4 decreases cell proliferation and alters leaf shape in Arabidopsis thaliana
Visit the PubMed ArticleVascular systems were also strongly stained throughout the whole plant ... AHK4:GUS
Nishimura C, Ohashi Y, Sato S, Kato T, Tabata S, Ueguchi C - Histidine kinase homologs that act as cytokinin receptors possess overlapping functions in the regulation of shoot and root growth in Arabidopsis
Visit the PubMed Articlestrong staining was mainly localized in ... growing leaf ... AHK3:GUS
Nishimura C, Ohashi Y, Sato S, Kato T, Tabata S, Ueguchi C - Histidine kinase homologs that act as cytokinin receptors possess overlapping functions in the regulation of shoot and root growth in Arabidopsis
Visit the PubMed ArticleIn various organs, the AHK genes are expressed nearly ubiquitously but at low levels. In seedlings, strong staining was mainly localized in meristematic tissues, such as the SAM ... AHK4:GUS
Nishimura C, Ohashi Y, Sato S, Kato T, Tabata S, Ueguchi C - Histidine kinase homologs that act as cytokinin receptors possess overlapping functions in the regulation of shoot and root growth in Arabidopsis
Visit the PubMed Articlestrong staining was mainly localized in ... growing leaf ... AHK2:GUS
Nishimura C, Ohashi Y, Sato S, Kato T, Tabata S, Ueguchi C - Histidine kinase homologs that act as cytokinin receptors possess overlapping functions in the regulation of shoot and root growth in Arabidopsis
Visit the PubMed ArticleAHK3:GUS ... Vascular systems were also strongly stained throughout the whole plant
Nishimura C, Ohashi Y, Sato S, Kato T, Tabata S, Ueguchi C - Histidine kinase homologs that act as cytokinin receptors possess overlapping functions in the regulation of shoot and root growth in Arabidopsis
Visit the PubMed ArticleAHK2:GUS ... Vascular systems were also strongly stained throughout the whole plant
Nishimura C, Ohashi Y, Sato S, Kato T, Tabata S, Ueguchi C - Histidine kinase homologs that act as cytokinin receptors possess overlapping functions in the regulation of shoot and root growth in Arabidopsis
Visit the PubMed ArticleIn various organs, the AHK genes are expressed nearly ubiquitously but at low levels. In seedlings, strong staining was mainly localized in meristematic tissues, such as the SAM ... AHK2:GUS
Nishimura C, Ohashi Y, Sato S, Kato T, Tabata S, Ueguchi C - Histidine kinase homologs that act as cytokinin receptors possess overlapping functions in the regulation of shoot and root growth in Arabidopsis
Visit the PubMed ArticleIn various organs, the AHK genes are expressed nearly ubiquitously but at low levels. In seedlings, strong staining was mainly localized in meristematic tissues, such as the SAM ... AHK3:GUS
Nishimura C, Ohashi Y, Sato S, Kato T, Tabata S, Ueguchi C - Histidine kinase homologs that act as cytokinin receptors possess overlapping functions in the regulation of shoot and root growth in Arabidopsis
Visit the PubMed Articlestrong staining was mainly localized in ... growing leaf ... AHK4:GUS
Nishimura C, Ohashi Y, Sato S, Kato T, Tabata S, Ueguchi C - Histidine kinase homologs that act as cytokinin receptors possess overlapping functions in the regulation of shoot and root growth in Arabidopsis
Visit the PubMed Articleno PHB expression was detected in AS1>>KAN2 leaves
Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities
Visit the PubMed Articlein wild type, PHB mRNA was localized to the SAM
Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities
Visit the PubMed ArticlePHB was localized to the adaxial regions of wild-type leaves
Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities
Visit the PubMed ArticleThe use of promoter-reporter gene fusions suggested that KAN ... phloem-associated vascular bundle expression
Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities
Visit the PubMed ArticlePHB was expressed throughout kan1 kan2 kan3 leaf primordia, although there was still a gradient with expression highest towards the adaxial side
Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities
Visit the PubMed Articlekan1 kan2 syd ... FIL expression was greatly reduced in this background
Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities
Visit the PubMed ArticleYAB3 ... β-glucuronidase (GUS) activity was undetectable in the kan1-2 kan2-1 kan3-1 yab3-2
Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities
Visit the PubMed Articlein AS1>>KAN2 ... a low level of PHB expression was still present in the apical meristem
Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities
Visit the PubMed ArticleThe use of promoter-reporter gene fusions suggested that KAN1 ... phloem-associated vascular bundle expression
Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities
Visit the PubMed Articleheart stage of embryogenesis, KAN1 ... expression pattern in the abaxial basal portion of emerging cotyledon primordia
Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities
Visit the PubMed Articleheart stage of embryogenesis ... KAN2 ... expression pattern in the abaxial basal portion of emerging cotyledon primordia
Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities
Visit the PubMed ArticleKAN1 is expressed in the abaxial regions of all lateral organs
Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities
Visit the PubMed Articlein wild type, PHB mRNA ... Later expression was confined to the provascular and vascular tissues of leaves and stems
Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities
Visit the PubMed ArticleThe use of promoter-reporter gene fusions suggested that KAN ... phloem-associated vascular bundle expression
Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities
Visit the PubMed Articlekan1 kan2 syd ... PHB was expressed throughout the developing leaves
Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities
Visit the PubMed ArticleKAN4 appears to have a more limited expression pattern in the ovules
Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities
Visit the PubMed ArticleYAB3 expression ... in an otherwise wild-type background, GUS activity was detected in the abaxial regions of developing yab3-2 leaves
Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities
Visit the PubMed ArticleFIL expression was detected throughout young AS1>>KAN2 leaf primordia (Fig. 3I). However, unlike wild-type leaves in which FIL expression is maintained in the marginal abaxial regions (Fig. 3H), FIL expression in AS1>>KAN2 was transient, only being detected in one leaf per apex
Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities
Visit the PubMed Articleheart stage of embryogenesis ... KAN3 ... expression pattern in the abaxial basal portion of emerging cotyledon primordia
Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities
Visit the PubMed Articlein wild type, PHB mRNA ... was restricted to the adaxial domain as developing primordia separated from the meristem
Eshed Y, Izhaki A, Baum SF, Floyd SK, Bowman JL - Asymmetric leaf development and blade expansion in Arabidopsis are mediated by KANADI and YABBY activities
Visit the PubMed ArticleIn ... situ hybridization showed that the timing and domain of AG mRNA accumulation in hua1-1 hua2-1 hen3-1 flowers were normal
Wang W, Chen X - HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis
Visit the PubMed ArticleGUS activity ... showed that HEN3 is expressed in young, presumably dividing, tissue, such as ... young leaves
Wang W, Chen X - HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis
Visit the PubMed Articlein hua1-1 hua2-1 hen3-1 flowers ... AG protein abundance was similar between hua1-1 hua2-1 ... flowers
Wang W, Chen X - HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis
Visit the PubMed Articlein the ... hua1-1 hua2-1 hen3-1 flowers ... The abundance of ... AP2 ... was also increased
Wang W, Chen X - HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis
Visit the PubMed Articlein the ... hua1-1 hua2-1 hen3-1 flowers ... The abundance of AP1 ... was also increased
Wang W, Chen X - HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis
Visit the PubMed Articlehua1-1 hua2-1 hen3-1 flowers were defective in the spatial control of AP1 expression
Wang W, Chen X - HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis
Visit the PubMed ArticleWe performed in situ hybridization to determine the localization of HEN3 RNA in inflorescence meristems and in flowers. HEN3 RNA was present throughout the inflorescence meristem, the inflorescence stem and young flowers
Wang W, Chen X - HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis
Visit the PubMed ArticleIn situ hybridization showed that histone H4, a gene known to be expressed in a cell cycle-dependent manner, was expressed similarly in wild-type and hen3-1 SAMs
Wang W, Chen X - HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis
Visit the PubMed Articlehua1-1 hua2-1 hen3-1 flowers were defective in the ... temporal control of WUS expression in flower development
Wang W, Chen X - HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis
Visit the PubMed ArticleRNA filter hybridization showed that HEN3 RNA was present in ... stems
Wang W, Chen X - HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis
Visit the PubMed ArticleGUS activity showed that HEN3 is expressed in young, presumably dividing, tissue, such as shoot ... tips
Wang W, Chen X - HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis
Visit the PubMed ArticleHEN3 RNA ... was more abundant in ag-3 inflorescences
Wang W, Chen X - HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis
Visit the PubMed ArticleGUS activity ... showed that HEN3 is expressed in young, presumably dividing, tissue, such as ... flowers
Wang W, Chen X - HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis
Visit the PubMed ArticleWe performed RNA filter hybridization to determine whether the hen3-1 mutation also results in reduced AG RNA levels in the hua1-1 hua2-1 background ... Three AG RNAs, RNA1, RNA2 and AG mRNA, can be detected in hua1-1 hua2-1 and hua1-1 hua2-1 hen3-1 flowers ... hen3-1 resulted in an increase in abundance in both the intron-containing RNAs and the mRNA in the hua1-1 hua2-1 background
Wang W, Chen X - HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis
Visit the PubMed ArticleRNA filter hybridization showed that HEN3 RNA was present in leaves
Wang W, Chen X - HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis
Visit the PubMed ArticleIn situ hybridization showed that histone H4, a gene known to be expressed in a cell cycle-dependent manner, was expressed similarly in wild-type and hen3-1 ... leaf primordia
Wang W, Chen X - HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis
Visit the PubMed ArticleRNA filter hybridization showed that HEN3 RNA was present in ... inflorescences
Wang W, Chen X - HUA ENHANCER3 reveals a role for a cyclin-dependent protein kinase in the specification of floral organ identity in Arabidopsis
Visit the PubMed ArticleThe expression of the above- mentioned genes was quantified by real-time PCR in expanding leaves collected from ron2-1 ... homozygous plants ... AG expression was measured on the leaf samples described above and no significant differences with the wild type were found
Cnops G, Jover-Gil S, Peters JL, Neyt P, De Block S, Robles P, Ponce MR, Gerats T, Micol JL, Van Lijsebettens M - The rotunda2 mutants identify a role for the LEUNIG gene in vegetative leaf morphogenesis
Visit the PubMed ArticleThe expression of the above- mentioned genes was quantified by real-time PCR in expanding leaves collected from ... ron2-2 ... homozygous plants ... AS1 ... transcript levels were normal in the mutants and did not account for the reduction in STM transcript level
Cnops G, Jover-Gil S, Peters JL, Neyt P, De Block S, Robles P, Ponce MR, Gerats T, Micol JL, Van Lijsebettens M - The rotunda2 mutants identify a role for the LEUNIG gene in vegetative leaf morphogenesis
Visit the PubMed ArticleThe expression of the above- mentioned genes was quantified by real-time PCR in expanding leaves collected from ... ron2-2 ... homozygous plants ... STM expression level in each mutant tested was reduced 10–20-fold compared with that of the wild type
Cnops G, Jover-Gil S, Peters JL, Neyt P, De Block S, Robles P, Ponce MR, Gerats T, Micol JL, Van Lijsebettens M - The rotunda2 mutants identify a role for the LEUNIG gene in vegetative leaf morphogenesis
Visit the PubMed ArticleThe expression of the above- mentioned genes was quantified by real-time PCR in expanding leaves collected from ... lug- 1 homozygous plants ... AS2 transcript levels were normal in the mutants and did not account for the reduction in STM transcript level
Cnops G, Jover-Gil S, Peters JL, Neyt P, De Block S, Robles P, Ponce MR, Gerats T, Micol JL, Van Lijsebettens M - The rotunda2 mutants identify a role for the LEUNIG gene in vegetative leaf morphogenesis
Visit the PubMed ArticleThe expression of the above- mentioned genes was quantified by real-time PCR in expanding leaves collected from ron2-1 ... homozygous plants ... STM expression level in each mutant tested was reduced 10–20-fold compared with that of the wild type
Cnops G, Jover-Gil S, Peters JL, Neyt P, De Block S, Robles P, Ponce MR, Gerats T, Micol JL, Van Lijsebettens M - The rotunda2 mutants identify a role for the LEUNIG gene in vegetative leaf morphogenesis
Visit the PubMed ArticleThe expression of the above- mentioned genes was quantified by real-time PCR in expanding leaves collected from ... ron2-2 ... homozygous plants ... AS2 transcript levels were normal in the mutants and did not account for the reduction in STM transcript level
Cnops G, Jover-Gil S, Peters JL, Neyt P, De Block S, Robles P, Ponce MR, Gerats T, Micol JL, Van Lijsebettens M - The rotunda2 mutants identify a role for the LEUNIG gene in vegetative leaf morphogenesis
Visit the PubMed ArticleThe expression of the above- mentioned genes was quantified by real-time PCR in expanding leaves collected from ron2-1 ... homozygous plants ... AS2 transcript levels were normal in the mutants and did not account for the reduction in STM transcript level
Cnops G, Jover-Gil S, Peters JL, Neyt P, De Block S, Robles P, Ponce MR, Gerats T, Micol JL, Van Lijsebettens M - The rotunda2 mutants identify a role for the LEUNIG gene in vegetative leaf morphogenesis
Visit the PubMed ArticleThe expression of the above- mentioned genes was quantified by real-time PCR in expanding leaves collected from ... ron2-2 ... homozygous plants ... AG expression was measured on the leaf samples described above and no significant differences with the wild type were found
Cnops G, Jover-Gil S, Peters JL, Neyt P, De Block S, Robles P, Ponce MR, Gerats T, Micol JL, Van Lijsebettens M - The rotunda2 mutants identify a role for the LEUNIG gene in vegetative leaf morphogenesis
Visit the PubMed ArticleThe expression of the above- mentioned genes was quantified by real-time PCR in expanding leaves collected from ... lug- 1 homozygous plants ... AS1 ... transcript levels were normal in the mutants and did not account for the reduction in STM transcript level
Cnops G, Jover-Gil S, Peters JL, Neyt P, De Block S, Robles P, Ponce MR, Gerats T, Micol JL, Van Lijsebettens M - The rotunda2 mutants identify a role for the LEUNIG gene in vegetative leaf morphogenesis
Visit the PubMed ArticleThe expression of the above- mentioned genes was quantified by real-time PCR in expanding leaves collected from ... lug- 1 homozygous plants ... AG expression was measured on the leaf samples described above and no significant differences with the wild type were found
Cnops G, Jover-Gil S, Peters JL, Neyt P, De Block S, Robles P, Ponce MR, Gerats T, Micol JL, Van Lijsebettens M - The rotunda2 mutants identify a role for the LEUNIG gene in vegetative leaf morphogenesis
Visit the PubMed ArticleThe expression of the above- mentioned genes was quantified by real-time PCR in expanding leaves collected from ... lug- 1 homozygous plants ... STM expression level in each mutant tested was reduced 10–20-fold compared with that of the wild type
Cnops G, Jover-Gil S, Peters JL, Neyt P, De Block S, Robles P, Ponce MR, Gerats T, Micol JL, Van Lijsebettens M - The rotunda2 mutants identify a role for the LEUNIG gene in vegetative leaf morphogenesis
Visit the PubMed ArticleThe expression of the above- mentioned genes was quantified by real-time PCR in expanding leaves collected from ron2-1 ... homozygous plants ... AS1 ... transcript levels were normal in the mutants and did not account for the reduction in STM transcript level
Cnops G, Jover-Gil S, Peters JL, Neyt P, De Block S, Robles P, Ponce MR, Gerats T, Micol JL, Van Lijsebettens M - The rotunda2 mutants identify a role for the LEUNIG gene in vegetative leaf morphogenesis
Visit the PubMed Articlelevel of HLS1 protein was increased in response to ethylene treatment
Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis
Visit the PubMed ArticleThe in situ localization of ARF2 mRNA in hls1-1 mutants also revealed that the expression patterns of ARF2 in cotyledons and hypocotyl were not affected by the hls1-1 mutation
Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis
Visit the PubMed Articlelevel of ARF2 was ... not affected in hls1-1 mutant upon exposure to light
Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis
Visit the PubMed ArticleOur studies show that ARF2 protein levels were unchanged by treatment with various concentrations of auxin (Figure 4C), while auxin-inducible IAA1 mRNA was increased significantly by the same auxin treatment. These results suggest that the abundance of the ARF2 is not mediated by an indirect effect of ethylene on auxin levels
Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis
Visit the PubMed ArticleTransgenic seedlings that overexpress HLS1 have slightly lower ARF2 protein levels than that observed in wild-type
Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis
Visit the PubMed ArticleARF2 also showed expression in the cotyledons and the hypocotyl after hook opening upon exposure of etiolated seedlings to light
Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis
Visit the PubMed Articlelevel of ARF2 protein was decreased upon exposure to ethylene
Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis
Visit the PubMed Articlelevel of HLS1 transcription is increased by ethylene (Lehman et al., 1996)
Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis
Visit the PubMed ArticleARF2 mRNA was found to be present in ... etiolated seedlings ... in the ... leaf primordia
Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis
Visit the PubMed ArticleARF2 mRNA was found to be present in ... etiolated seedlings ... in the cotyledons
Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis
Visit the PubMed ArticleImmunoblot analyses of etiolated seedlings that were treated with ACC with or without MG132, a specific inhibitor of the proteasome-dependent protein degradation, revealed that MG132 suppressed the ethylene-dependent decrease in ARF2 protein levels (Figure 3C). These results strongly suggest that ethylene-mediated degradation of the ARF2 protein is via a proteasome-dependent pathway
Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis
Visit the PubMed ArticleInterestingly, the arf2-3 mutation partially restored DR5::GUS expression within cells located on the concave side of the hook in the arf2-3 hls1-1 double mutant in the presence of ethylene
Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis
Visit the PubMed Articlehistochemical staining of transgenic seedlings expressing pARF2::GUS:ARF2 in wild-type, hls1 mutant, and light-grown wild-type seedlings revealed that the ARF2 protein was mainly expressed in the apical portion of the hypocotyl and in the cotyledons, and these patterns were not affected by the hls1-1 mutation or exposure to light (Figure 2F)
Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis
Visit the PubMed Articlehistochemical staining of transgenic seedlings expressing pARF2::GUS:ARF2 in wild-type, hls1 mutant, and light-grown wild-type seedlings revealed that the ARF2 protein was mainly expressed in the apical portion of the hypocotyl and in the cotyledons, and these patterns were not affected by the hls1-1 mutation or exposure to light (Figure 2F)
Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis
Visit the PubMed ArticleImmunoblot analyses revealed that the level of HLS1 protein was decreased upon light exposure, even in the presence of the ethylene precursor ACC
Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis
Visit the PubMed ArticleARF2 mRNA was found to be present in the apical hook region of etiolated seedlings
Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis
Visit the PubMed ArticleSince neither expression patterns nor transcription levels of ARF2 (data not shown) were affected by the loss of HLS1 function
Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis
Visit the PubMed ArticleDR5::GUS was only expressed within cells located at the concave side of the hypocotyl hook (Figure 5), and the level of expression was enhanced by ethylene treatment. However, the expression level in the cotyledon was highly variable. In hls1-1 mutants, this highly localized expression pattern was completely abolished
Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis
Visit the PubMed Articlelevel of ARF2 was increased in wild-type seedlings ... upon exposure to light
Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis
Visit the PubMed ArticlePolyclonal antibodies that specifically recognize the ARF2 protein were generated (Figure 3A). Immunoblot analysis was used to detect ARF2 protein levels in 3-day-old etiolated seedlings. Consistent with the transgenic expression of pARF2::GUS:ARF2 in wild-type and hls1-1 mutant, the level of ARF2 protein was increased in the hls1-1 mutant
Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis
Visit the PubMed ArticleInterestingly, the intensity of GUS staining in etiolated hls1 mutants and in wild-type seedlings exposed to light was higher than that observed in etiolated wild-type seedlings (Figure 2F), suggesting that the level of GUS:ARF2 protein may be regulated by HLS1 functions
Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis
Visit the PubMed Articlearf2-3 had no effect on the expression pattern of DR5::GUS in wild-type plants
Li H, Johnson P, Stepanova A, Alonso JM, Ecker JR - Convergence of signaling pathways in the control of differential cell growth in Arabidopsis
Visit the PubMed ArticleIn older leaves (rosette) as well as in cauline leaves, SHN3 was mainly expressed in the central vein with lower expression in the entire blade
Aharoni A, Dixit S, Jetter R, Thoenes E, van Arkel G, Pereira A - The SHINE clade of AP2 domain transcription factors activates wax biosynthesis, alters cuticle properties, and confers drought tolerance when overexpressed in Arabidopsis
Visit the PubMed ArticleIn older leaves (rosette) as well as in cauline leaves, SHN3 was mainly expressed in the central vein with lower expression in the entire blade
Aharoni A, Dixit S, Jetter R, Thoenes E, van Arkel G, Pereira A - The SHINE clade of AP2 domain transcription factors activates wax biosynthesis, alters cuticle properties, and confers drought tolerance when overexpressed in Arabidopsis
Visit the PubMed ArticleSHN1/WIN1 ... constructs, which linked 2.0-kb DNA sequences upstream of the predicted ATG codon of each gene to the β-glucuronidase (GUS) reporter gene ... SHN1/WIN1 expression was detected in the inflorescence and root tissues but not in stem, mature rosette leaves, or cauline leaves
Aharoni A, Dixit S, Jetter R, Thoenes E, van Arkel G, Pereira A - The SHINE clade of AP2 domain transcription factors activates wax biosynthesis, alters cuticle properties, and confers drought tolerance when overexpressed in Arabidopsis
Visit the PubMed ArticleSHN1/WIN1 ... constructs, which linked 2.0-kb DNA sequences upstream of the predicted ATG codon of each gene to the β-glucuronidase (GUS) reporter gene ... GUS expression was observed ... in ... support cells of their trichomes
Aharoni A, Dixit S, Jetter R, Thoenes E, van Arkel G, Pereira A - The SHINE clade of AP2 domain transcription factors activates wax biosynthesis, alters cuticle properties, and confers drought tolerance when overexpressed in Arabidopsis
Visit the PubMed ArticleSHN1/WIN1 ... constructs, which linked 2.0-kb DNA sequences upstream of the predicted ATG codon of each gene to the β-glucuronidase (GUS) reporter gene ... GUS expression was observed ... in ... very young leaves in the rosette
Aharoni A, Dixit S, Jetter R, Thoenes E, van Arkel G, Pereira A - The SHINE clade of AP2 domain transcription factors activates wax biosynthesis, alters cuticle properties, and confers drought tolerance when overexpressed in Arabidopsis
Visit the PubMed ArticleThe SHN3 gene was most broadly expressed and was active in all plant organs. It showed expression in the vasculature and in the lateral root tip (Figure 9). When staining young 10-d-old seedlings, expression was detected in the support cells of trichomes present on the most newly formed leaves
Aharoni A, Dixit S, Jetter R, Thoenes E, van Arkel G, Pereira A - The SHINE clade of AP2 domain transcription factors activates wax biosynthesis, alters cuticle properties, and confers drought tolerance when overexpressed in Arabidopsis
Visit the PubMed ArticleRNA gel blot analysis of total RNAs, isolated from mock- or brassinolide-treated seedlings of wild-type ... revealed no detectable difference in the abundance of the TTL transcript between mock- and BR-treated seedlings
Nam KH, Li J - The Arabidopsis transthyretin-like protein is a potential substrate of BRASSINOSTEROID-INSENSITIVE 1
Visit the PubMed ArticleTo obtain a better picture of tissue specificity of TTL gene expression, we constructed a TTL-β-glucuronidase (GUS) reporter gene by fusing a 1.6-kb genomic fragment containing the native TTL promoter to a GUS reporter gene. As shown in Figures 3C to 3F, the GUS activity was detected mainly in ... leaf primordia
Nam KH, Li J - The Arabidopsis transthyretin-like protein is a potential substrate of BRASSINOSTEROID-INSENSITIVE 1
Visit the PubMed ArticleTo obtain a better picture of tissue specificity of TTL gene expression, we constructed a TTL-β-glucuronidase (GUS) reporter gene by fusing a 1.6-kb genomic fragment containing the native TTL promoter to a GUS reporter gene. As shown in Figures 3C to 3F, the GUS activity was detected mainly in ... newly expanding young leaves
Nam KH, Li J - The Arabidopsis transthyretin-like protein is a potential substrate of BRASSINOSTEROID-INSENSITIVE 1
Visit the PubMed ArticleConfocal microscopic analysis of the resulting TTL:GFP transgenic plants revealed a similar green fluorescent pattern between TTL:GFP and BRI1:GFP, indicating that TTL was indeed associated with the plasma membrane ... The membrane association of TTL was also confirmed by a cell fractionation assay. Membrane proteins of a transgenic TTL:GFP plant were separated from cytosolic proteins by differential centrifugation, these proteins were separated by SDS-PAGE, and the presence of TTL:GFP in the two fractions was detected by immunoblotting using a commercial anti-GFP antiserum. As shown in Figure 4B, both BRI1:GFP and TTL:GFP were mainly in the membrane fraction
Nam KH, Li J - The Arabidopsis transthyretin-like protein is a potential substrate of BRASSINOSTEROID-INSENSITIVE 1
Visit the PubMed ArticleTTL was expressed in all the tissues that we analyzed, with the flower buds and elongating inflorescence stems accumulating the highest levels of the TTL transcripts
Nam KH, Li J - The Arabidopsis transthyretin-like protein is a potential substrate of BRASSINOSTEROID-INSENSITIVE 1
Visit the PubMed ArticleGIF1 was ... expressed ... weakly in leaves
Kim JH, Kende H - A transcriptional coactivator, AtGIF1, is involved in regulating leaf growth and morphology in Arabidopsis
Visit the PubMed ArticleOnion epidermal cells transformed transiently to express GIF1 fused to enhanced yellow fluorescent protein (EYFP) also showed nuclear speckles, which were distributed throughout the nucleus in 21 of 40 transformants
Kim JH, Kende H - A transcriptional coactivator, AtGIF1, is involved in regulating leaf growth and morphology in Arabidopsis
Visit the PubMed ArticleGIF1 was strongly expressed in ... shoot tips containing the shoot apical meristem
Kim JH, Kende H - A transcriptional coactivator, AtGIF1, is involved in regulating leaf growth and morphology in Arabidopsis
Visit the PubMed Articlerdr6-11 ... expression was increased ... SPL3
Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS - SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis
Visit the PubMed Articlerdr6-11 ... we examined the accumulation of miR156 ... Little or no difference in the levels of these miRNAs was observed
Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS - SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis
Visit the PubMed ArticleAt5g18040 ... mRNA accumulates in sgs3-11 and rdr6-11, but not in zip-1
Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS - SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis
Visit the PubMed Articlesgs3-11 ... expression was increased ... of ARF3/ETTIN
Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS - SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis
Visit the PubMed Articlerdr6-11 ... expression was increased ... of ARF3/ETTIN
Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS - SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis
Visit the PubMed Articlezip-1 ... expression was increased ... of ... SPL3
Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS - SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis
Visit the PubMed Articlezip-1 ... expression was increased ... of ARF3/ETTIN
Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS - SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis
Visit the PubMed ArticleAt1g63130 ... mRNA accumulates in sgs3-11 and rdr6-11, but not in zip-1
Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS - SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis
Visit the PubMed Articlezip-1 ... expression was increased ... of ... ARF4
Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS - SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis
Visit the PubMed ArticleAt1g63130 ... mRNA accumulates in sgs3-11
Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS - SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis
Visit the PubMed ArticleAt5g18040 ... mRNA accumulates in sgs3-11
Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS - SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis
Visit the PubMed Articlesgs3-11 ... expression was increased ... of ... ARF4
Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS - SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis
Visit the PubMed ArticleAt5g18040 ... mRNA accumulates in ... rdr6-11
Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS - SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis
Visit the PubMed Articlesgs3-11 ... expression was increased ... of ... SPL3
Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS - SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis
Visit the PubMed Articlerdr6-11 ... expression was increased ... ARF4
Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS - SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis
Visit the PubMed ArticleAt1g63130 ... mRNA accumulates in ... rdr6-11
Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS - SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis
Visit the PubMed Articlesgs3-11 ... we examined the accumulation of miR156 ... Little or no difference in the levels of these miRNAs was observed
Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS - SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis
Visit the PubMed Articlezip-1 ... we examined the accumulation of miR156 ... Little or no difference in the levels of these miRNAs was observed
Peragine A, Yoshikawa M, Wu G, Albrecht HL, Poethig RS - SGS3 and SGS2/SDE1/RDR6 are required for juvenile development and the production of trans-acting siRNAs in Arabidopsis
Visit the PubMed ArticleIn contrast with ATB2 and LHY, several other leaders tested drove translation as effectively in eif3h-1 as in the wild type; these included the leaders of ABSCISIC ACID INSENSITIVE5 (ABI5), the omega (Ω) leader of Tobacco mosaic virus (TMV), the small subunit of Rubisco (RBCS), and the bZip protein HY5 (Figure 7G). A moderate, yet significant (P < 0.05) difference in expression was observed for chlorophyll a/b binding protein 2 (CAB2
Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h
Visit the PubMed Articlewe examined whether and how eIF3h might affect the translation of specific mRNAs. Selected 5′ leader sequences were compared for their translational capacity between eif3h mutants and the wild type using a dual-luciferase reporter assay ... the activity of the FLUC reporter gene fused to the ATB2 leader was reduced fourfold, on average, in the eif3h-1 mutant compared with the wild type
Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h
Visit the PubMed Articlewe examined whether and how eIF3h might affect the translation of specific mRNAs. Selected 5′ leader sequences were compared for their translational capacity between eif3h mutants and the wild type using a dual-luciferase reporter assay ... the activity of the FLUC reporter gene fused to the ATB2 leader was reduced fourfold, on average, in the eif3h-1 mutant compared with the wild type ... Inhibition of translation was also observed for the leader of the myb protein LATE ELONGATED HYPOCOTYL (LHY
Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h
Visit the PubMed ArticleVice versa, no difference was detected in the level of two eIF3 subunits, eIF3b and eIF3h, in csn1 or csn7 mutants
Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h
Visit the PubMed ArticleIn contrast with ATB2 and LHY, several other leaders tested drove translation as effectively in eif3h-1 as in the wild type; these included the leaders of ABSCISIC ACID INSENSITIVE5 (ABI5), the omega (Ω) leader of Tobacco mosaic virus (TMV), the small subunit of Rubisco (RBCS), and the bZip protein HY5
Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h
Visit the PubMed ArticleIn wild-type plants, the translational activity of the ATB2:FLUC reporter was specifically repressed by sucrose (Figure 9C), consistent with previous conclusions (Rook et al., 1998a); for comparison, the HY5 leader was insensitive to sucrose
Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h
Visit the PubMed ArticleMorphological phenotypes of the eif3h-1 mutant ... suggested an altered auxin response. Consistent with this notion, the mRNA levels of two auxin-inducible genes were elevated slightly in the absence (IAA3 and IAA14) or the presence (IAA3) of added auxin, whereas the level of IAA17 remained normal (Figure 3G
Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h
Visit the PubMed ArticleIn contrast with ATB2 and LHY, several other leaders tested drove translation as effectively in eif3h-1 as in the wild type; these included the leaders of ABSCISIC ACID INSENSITIVE5 (ABI5), the omega (Ω) leader of Tobacco mosaic virus (TMV), the small subunit of Rubisco (RBCS), and the bZip protein HY5
Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h
Visit the PubMed ArticleVice versa, no difference was detected in the level of two eIF3 subunits, eIF3b and eIF3h, in csn1 or csn7 mutants
Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h
Visit the PubMed ArticleTo seek independent evidence for a role of eIF3h in translation initiation on specific mRNAs, we compared eif3h-1 mutants and the wild type for the distribution of specific mRNAs in sucrose gradients designed to fractionate polysomal and nonpolysomal RNA, followed by detection of specific transcripts by RT-PCR ... a reduction in the polysome loading of the endogenous ATB2 mRNA was detected in the eif3h-1 mutant
Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h
Visit the PubMed ArticleMorphological phenotypes of the eif3h-1 mutant ... suggested an altered auxin response. Consistent with this notion, the mRNA levels of two auxin-inducible genes were elevated slightly in the absence (IAA3 and IAA14) or the presence (IAA3) of added auxin, whereas the level of IAA17 remained normal (Figure 3G
Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h
Visit the PubMed ArticleVice versa, no difference was detected in the level of two eIF3 subunits, eIF3b and eIF3h, in csn1 or csn7 mutants
Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h
Visit the PubMed ArticleVice versa, no difference was detected in the level of two eIF3 subunits, eIF3b and eIF3h, in csn1 or csn7 mutants
Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h
Visit the PubMed ArticleTo investigate the role of eIF3h in Arabidopsis, we isolated a recessive mutant allele (eif3h-1) carrying a T-DNA insertion in the 10th exon (Figure 2A). A truncated eIF3h-related protein was detected in this allele
Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h
Visit the PubMed ArticleAlthough eIF3h could associate with the CSN (Karniol et al., 1998), loss of eIF3h did not affect accumulation of its CSN4 or CSN5 subunits (Figure 4C
Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h
Visit the PubMed ArticleMorphological phenotypes of the eif3h-1 mutant ... suggested an altered auxin response. Consistent with this notion, the mRNA levels of two auxin-inducible genes were elevated slightly in the absence (IAA3 and IAA14) or the presence (IAA3) of added auxin, whereas the level of IAA17 remained normal (Figure 3G
Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h
Visit the PubMed ArticleIn wild-type plants, the translational activity of the ATB2:FLUC reporter was specifically repressed by sucrose (Figure 9C), consistent with previous conclusions (Rook et al., 1998a); for comparison, the HY5 leader was insensitive to sucrose
Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h
Visit the PubMed ArticleTo seek independent evidence for a role of eIF3h in translation initiation on specific mRNAs, we compared eif3h-1 mutants and the wild type for the distribution of specific mRNAs in sucrose gradients designed to fractionate polysomal and nonpolysomal RNA, followed by detection of specific transcripts by RT-PCR ... For comparison, the rbcS mRNA did not significantly change its distribution between polysomal and nonpolysomal fractions
Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h
Visit the PubMed ArticleAlthough eIF3h could associate with the CSN (Karniol et al., 1998), loss of eIF3h did not affect accumulation of its CSN4 or CSN5 subunits (Figure 4C
Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h
Visit the PubMed ArticleThe eIF3h protein accumulated in all organs tested yet was most abundant in roots and flowers
Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h
Visit the PubMed ArticleMorphological phenotypes of the eif3h-1 mutant ... suggested an altered auxin response. Consistent with this notion, the mRNA levels of two auxin-inducible genes were elevated slightly in the absence (IAA3 and IAA14) or the presence (IAA3) of added auxin, whereas the level of IAA17 remained normal (Figure 3G
Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h
Visit the PubMed ArticleIn contrast with ATB2 and LHY, several other leaders tested drove translation as effectively in eif3h-1 as in the wild type; these included the leaders of ABSCISIC ACID INSENSITIVE5 (ABI5), the omega (Ω) leader of Tobacco mosaic virus (TMV), the small subunit of Rubisco (RBCS), and the bZip protein HY5
Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h
Visit the PubMed ArticleAn eIF3h protein truncated by ∼2 kD accumulated in the eif3h-2 mutant
Kim TH, Kim BH, Yahalom A, Chamovitz DA, von Arnim AG - Translational regulation via 5' mRNA leader sequences revealed by mutational analysis of the Arabidopsis translation initiation factor subunit eIF3h
Visit the PubMed ArticleBOP1 ... is expressed at very high levels in the petiole relative to the blade
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE1 encodes a BTB/POZ domain protein required for leaf morphogenesis in Arabidopsis thaliana
Visit the PubMed ArticleBOP1 transcripts are also present along the margin of young leaves, where the adaxial and abax- ial domains juxtapose
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE1 encodes a BTB/POZ domain protein required for leaf morphogenesis in Arabidopsis thaliana
Visit the PubMed ArticleAfter germination, BOP1 transcripts ... are present in the rosette leaf primordia
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE1 encodes a BTB/POZ domain protein required for leaf morphogenesis in Arabidopsis thaliana
Visit the PubMed ArticleThe first stage at which BOP1 transcripts can be relia- bly detected is the torpedo stage of embryogenesis, when BOP1 is found in two discrete foci at the base of the develop- ing cotyledons, specifically on the adaxial side near the bound- ary with the shoot apical meristem
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE1 encodes a BTB/POZ domain protein required for leaf morphogenesis in Arabidopsis thaliana
Visit the PubMed ArticleBOP1 appears to be transcribed at the same relative level in whole rosette leaves regardless of age
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE1 encodes a BTB/POZ domain protein required for leaf morphogenesis in Arabidopsis thaliana
Visit the PubMed ArticleIn maturing leaves, a low level of BOP1 expression becomes detectable across the adaxial por- tion of the leaf
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE1 encodes a BTB/POZ domain protein required for leaf morphogenesis in Arabidopsis thaliana
Visit the PubMed ArticleAs the leaves grow out, BOP1 is expressed strongly yet in a highly restricted pattern at the base of the leaf primordia on the adaxial side adjacent to the SAM
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE1 encodes a BTB/POZ domain protein required for leaf morphogenesis in Arabidopsis thaliana
Visit the PubMed ArticleIn bop1-1 mutant plants, BOP1 transcription is elevated in all tissues compared with the wild type and becomes readily detectable in cotyledons and cauline leaves (Fig. 5B), consistent with the manifestation of phenotypes in these tissues
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE1 encodes a BTB/POZ domain protein required for leaf morphogenesis in Arabidopsis thaliana
Visit the PubMed ArticleAfter germination, BOP1 transcripts are not observed in the SAM
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE1 encodes a BTB/POZ domain protein required for leaf morphogenesis in Arabidopsis thaliana
Visit the PubMed ArticleBOP1 is expressed at barely detectable levels in cotyledons
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE1 encodes a BTB/POZ domain protein required for leaf morphogenesis in Arabidopsis thaliana
Visit the PubMed Articleupon AtE2Fa over-expression ... we also observed ele- vated expression of CycB1, a mitotic cyclin, sug- gesting the involvement of AtE2Fa for further cell cycle progression
He SS, Liu J, Xie Z, O'Neill D, Dotson S - Arabidopsis E2Fa plays a bimodal role in regulating cell division and cell growth
Visit the PubMed Articleupon AtE2Fa over-expression ... we found that the expression of several cell cycle inhibitor genes, including ... KRP3 ... was also up- regulated
He SS, Liu J, Xie Z, O'Neill D, Dotson S - Arabidopsis E2Fa plays a bimodal role in regulating cell division and cell growth
Visit the PubMed ArticleThe positive cell cycle factors up- regulated upon AtE2Fa over-expression included genes for G1/S transition (CycD2)
He SS, Liu J, Xie Z, O'Neill D, Dotson S - Arabidopsis E2Fa plays a bimodal role in regulating cell division and cell growth
Visit the PubMed ArticleThe positive cell cycle factors up- regulated upon AtE2Fa over-expression included genes for ... DNA synthesis (RNR1
He SS, Liu J, Xie Z, O'Neill D, Dotson S - Arabidopsis E2Fa plays a bimodal role in regulating cell division and cell growth
Visit the PubMed Articleupon AtE2Fa over-expression ... we found that the expression of several cell cycle inhibitor genes, including RB1 ... was also up- regulated
He SS, Liu J, Xie Z, O'Neill D, Dotson S - Arabidopsis E2Fa plays a bimodal role in regulating cell division and cell growth
Visit the PubMed Articleupon AtE2Fa over-expression ... we found that the expression of several cell cycle inhibitor genes, including ... KRP5, was also up- regulated
He SS, Liu J, Xie Z, O'Neill D, Dotson S - Arabidopsis E2Fa plays a bimodal role in regulating cell division and cell growth
Visit the PubMed ArticleComparison of the expression levels in bulked leaf samples revealed that, while the expression of ATPK1 was not changed ... upon the over-expression of AtE2Fa
He SS, Liu J, Xie Z, O'Neill D, Dotson S - Arabidopsis E2Fa plays a bimodal role in regulating cell division and cell growth
Visit the PubMed ArticleThe positive cell cycle factors up- regulated upon AtE2Fa over-expression included genes for ... DNA synthesis ... PCNA
He SS, Liu J, Xie Z, O'Neill D, Dotson S - Arabidopsis E2Fa plays a bimodal role in regulating cell division and cell growth
Visit the PubMed ArticleComparison of the expression levels in bulked leaf samples revealed that ... the expression of ATPK19 was increased upon the over-expression of AtE2Fa
He SS, Liu J, Xie Z, O'Neill D, Dotson S - Arabidopsis E2Fa plays a bimodal role in regulating cell division and cell growth
Visit the PubMed ArticleIn wild-type first leaves, DR5:GUS appears 3 DAG in a single cell at the leaf distal tip (Table 4, Figure 5A). In mutant first leaves, DR5:GUS is evident with similar timing. However, the position is often asymmetric relative to the leaf, shifted to one side of the distal tip (Table 4, Figures 5B and 5C). Subsequent DR5:GUS expression in mutant leaf blades is slightly later than in the wild type (Table 4), and no differences in pattern are evident, although expression within the blade at these stages is very transient, so that pattern differences would be difficult to detect (Figures 5D to 5F). At this stage, distal tip expression coincides with the distal margin gap, and in mutant leaves, both sometimes appear asymmetrically placed (Figure 5E). Asymmetric DR5:GUS expression is especially evident when expression begins in hydathodes (Table 4). In wild-type first leaves, hydathode expression usually appears in two points at equivalent positions along the leaf margin (Figure 5G). By contrast, expression in both as1-16 and as2-1 may begin at just one point and be followed by expression in another one or two, often unequally positioned points in as2-1 leaves (Figures 5H and 5I). Expression of DR5:GUS at this stage coincides with margin gaps (Figures 5J to 5L), and the margin gaps seem to contain more cells in the mutants than the wild type (cf. Figures 5K and 5L to 5J
Zgurski JM, Sharma R, Bolokoski DA, Schultz EA - Asymmetric auxin response precedes asymmetric growth and differentiation of asymmetric leaf1 and asymmetric leaf2 Arabidopsis leaves
Visit the PubMed ArticleConsistent with basipetal decrease in cell division (Donnelly et al., 1999), at 5 DAG, cycB1:GUS expression is diminishing in the distal half of both wild-type and mutant leaves, and the number of cells expressing is again lower in as2-1
Zgurski JM, Sharma R, Bolokoski DA, Schultz EA - Asymmetric auxin response precedes asymmetric growth and differentiation of asymmetric leaf1 and asymmetric leaf2 Arabidopsis leaves
Visit the PubMed ArticleAt 4 DAG, cycB1:GUS ... number of cells expressing the reporter gene is reduced in both halves of as2-1 leaves
Zgurski JM, Sharma R, Bolokoski DA, Schultz EA - Asymmetric auxin response precedes asymmetric growth and differentiation of asymmetric leaf1 and asymmetric leaf2 Arabidopsis leaves
Visit the PubMed ArticleIn wild-type first leaves, DR5:GUS appears 3 DAG in a single cell at the leaf distal tip (Table 4, Figure 5A). In mutant first leaves, DR5:GUS is evident with similar timing. However, the position is often asymmetric relative to the leaf, shifted to one side of the distal tip (Table 4, Figures 5B and 5C). Subsequent DR5:GUS expression in mutant leaf blades is slightly later than in the wild type (Table 4), and no differences in pattern are evident, although expression within the blade at these stages is very transient, so that pattern differences would be difficult to detect (Figures 5D to 5F). At this stage, distal tip expression coincides with the distal margin gap, and in mutant leaves, both sometimes appear asymmetrically placed (Figure 5E). Asymmetric DR5:GUS expression is especially evident when expression begins in hydathodes (Table 4). In wild-type first leaves, hydathode expression usually appears in two points at equivalent positions along the leaf margin (Figure 5G). By contrast, expression in both as1-16 and as2-1 may begin at just one point and be followed by expression in another one or two, often unequally positioned points in as2-1 leaves (Figures 5H and 5I). Expression of DR5:GUS at this stage coincides with margin gaps (Figures 5J to 5L), and the margin gaps seem to contain more cells in the mutants than the wild type (cf. Figures 5K and 5L to 5J
Zgurski JM, Sharma R, Bolokoski DA, Schultz EA - Asymmetric auxin response precedes asymmetric growth and differentiation of asymmetric leaf1 and asymmetric leaf2 Arabidopsis leaves
Visit the PubMed ArticleAt 4 DAG, cycB1:GUS expression is similar in distal and proximal leaf halves and shows no significant difference between the wild type and as1-16
Zgurski JM, Sharma R, Bolokoski DA, Schultz EA - Asymmetric auxin response precedes asymmetric growth and differentiation of asymmetric leaf1 and asymmetric leaf2 Arabidopsis leaves
Visit the PubMed Articleit has been reported that rosette leaves of transgenic plants carrying the ABI4::GUS construct have no detectable GUS activity (Arroyo et al. 2003
Kozuka T, Horiguchi G, Kim GT, Ohgishi M, Sakai T, Tsukaya H - The different growth responses of the Arabidopsis thaliana leaf blade and the petiole during shade avoidance are regulated by photoreceptors and sugar
Visit the PubMed ArticleIn seedlings overexpressing CYP72C1, the expression levels of TCH4 ... were reduced
Takahashi N, Nakazawa M, Shibata K, Yokota T, Ishikawa A, Suzuki K, Kawashima M, Ichikawa T, Shimada H, Matsui M - shk1-D, a dwarf Arabidopsis mutant caused by activation of the CYP72C1 gene, has altered brassinosteroid levels
Visit the PubMed ArticleThe CYP72C1 gene was expressed more strongly in the dark than in the light
Takahashi N, Nakazawa M, Shibata K, Yokota T, Ishikawa A, Suzuki K, Kawashima M, Ichikawa T, Shimada H, Matsui M - shk1-D, a dwarf Arabidopsis mutant caused by activation of the CYP72C1 gene, has altered brassinosteroid levels
Visit the PubMed ArticleTo confirm the expression of CYP72C1, we performed RT-PCR. We prepared total RNA from flowers, siliques, stems, cauline leaves and rosette leaves of 4-week-old plants and from roots in liquid culture. We observed CYP72C1 expression in all tissues examined and relatively strong expression in siliques and roots (Figure 5j)
Takahashi N, Nakazawa M, Shibata K, Yokota T, Ishikawa A, Suzuki K, Kawashima M, Ichikawa T, Shimada H, Matsui M - shk1-D, a dwarf Arabidopsis mutant caused by activation of the CYP72C1 gene, has altered brassinosteroid levels
Visit the PubMed ArticleIn seedlings overexpressing CYP72C1, the expression levels of ... BAS1 were reduced
Takahashi N, Nakazawa M, Shibata K, Yokota T, Ishikawa A, Suzuki K, Kawashima M, Ichikawa T, Shimada H, Matsui M - shk1-D, a dwarf Arabidopsis mutant caused by activation of the CYP72C1 gene, has altered brassinosteroid levels
Visit the PubMed ArticleIn seedlings overexpressing CYP72C1 ... CPD expression was slightly increased
Takahashi N, Nakazawa M, Shibata K, Yokota T, Ishikawa A, Suzuki K, Kawashima M, Ichikawa T, Shimada H, Matsui M - shk1-D, a dwarf Arabidopsis mutant caused by activation of the CYP72C1 gene, has altered brassinosteroid levels
Visit the PubMed Articletransgenic plants containing the 2.0-kb upstream promoter region of the CYP72C1 gene fused to the β-glucuronidase gene (GUS ... in light-grown seedlings it was seen in cotyledons
Takahashi N, Nakazawa M, Shibata K, Yokota T, Ishikawa A, Suzuki K, Kawashima M, Ichikawa T, Shimada H, Matsui M - shk1-D, a dwarf Arabidopsis mutant caused by activation of the CYP72C1 gene, has altered brassinosteroid levels
Visit the PubMed ArticleIn bop1-1 plants, KNAT1::GUS was expressed in a pattern similar to that in wild-type plants, but in more expanded regions
Norberg M, Holmlund M, Nilsson O - The BLADE ON PETIOLE genes act redundantly to control the growth and development of lateral organs
Visit the PubMed ArticleExamination of the expression pattern in single leaves of bop1-1 showed that, unlike in wild-type leaves, KNAT1::GUS was detected in the petioles of cotyledons and rosette leaves
Norberg M, Holmlund M, Nilsson O - The BLADE ON PETIOLE genes act redundantly to control the growth and development of lateral organs
Visit the PubMed ArticleKNAT6 transcript ... levels ... in the cotyledons and rosette leaves of bop1-1 seedlings were significantly higher than those in wild-type seedlings
Norberg M, Holmlund M, Nilsson O - The BLADE ON PETIOLE genes act redundantly to control the growth and development of lateral organs
Visit the PubMed Articlein the case of the KNAT2 that showed only a slightly increased expression in the mutant rosette leaf
Norberg M, Holmlund M, Nilsson O - The BLADE ON PETIOLE genes act redundantly to control the growth and development of lateral organs
Visit the PubMed Articlethe KNAT1 ... transcript ... levels ... in the cotyledons and rosette leaves of bop1-1 seedlings were significantly higher than those in wild-type seedlings
Norberg M, Holmlund M, Nilsson O - The BLADE ON PETIOLE genes act redundantly to control the growth and development of lateral organs
Visit the PubMed Articlethe KNAT1 ... there were no notable differences between the wild-type and mutant SAM regions in the expression of these genes
Norberg M, Holmlund M, Nilsson O - The BLADE ON PETIOLE genes act redundantly to control the growth and development of lateral organs
Visit the PubMed ArticleKNAT2 ... there were no notable differences between the wild-type and mutant SAM regions in the expression of these genes
Norberg M, Holmlund M, Nilsson O - The BLADE ON PETIOLE genes act redundantly to control the growth and development of lateral organs
Visit the PubMed ArticleKNAT6 ... there were no notable differences between the wild-type and mutant SAM regions in the expression of these genes
Norberg M, Holmlund M, Nilsson O - The BLADE ON PETIOLE genes act redundantly to control the growth and development of lateral organs
Visit the PubMed ArticleTo determine the subcellular localization of the BOP2 protein, a 35S:BOP2�green fluorescent protein (GFP) transgene was introduced into bop1 bop2 plants by Agrobacterium-mediated transformation. Partial complementation of the phenotype conferred by bop1 bop2 was obtained for many of the resulting transgenic plant lines, indicating that the fusion protein had biological activity (data not shown). Confocal laser scanning of a seedling at 4 d after germination shows that in root cells, the BOP2-GFP fusion protein was localized to both the cytoplasm and the nucleus
Hepworth SR, Zhang Y, McKim S, Li X, Haughn GW - BLADE-ON-PETIOLE-dependent signaling controls leaf and floral patterning in Arabidopsis
Visit the PubMed ArticleBOP2 ... transcripts ... were detected in leaves, stems, apices, flowers, and roots but not in siliques
Hepworth SR, Zhang Y, McKim S, Li X, Haughn GW - BLADE-ON-PETIOLE-dependent signaling controls leaf and floral patterning in Arabidopsis
Visit the PubMed ArticleTo determine the subcellular localization of the BOP2 protein, a 35S:BOP2�green fluorescent protein (GFP) transgene was introduced into bop1 bop2 plants by Agrobacterium-mediated transformation. Partial complementation of the phenotype conferred by bop1 bop2 was obtained for many of the resulting transgenic plant lines, indicating that the fusion protein had biological activity (data not shown). Confocal laser scanning of a seedling at 4 d after germination shows that in root cells, the BOP2-GFP fusion protein was localized to both the cytoplasm and the nucleus
Hepworth SR, Zhang Y, McKim S, Li X, Haughn GW - BLADE-ON-PETIOLE-dependent signaling controls leaf and floral patterning in Arabidopsis
Visit the PubMed ArticleBOP1 ... transcripts ... were detected in leaves, stems, apices, flowers, and roots but not in siliques
Hepworth SR, Zhang Y, McKim S, Li X, Haughn GW - BLADE-ON-PETIOLE-dependent signaling controls leaf and floral patterning in Arabidopsis
Visit the PubMed ArticleThe expression of the ... ELO3 genes was monitored in different plant organs by RT-PCR: roots, young first leaves, whole seedlings (14 DAG), cotyledons, expanded first leaves, floral buds, and shoot apices (27 DAG). The three ELO genes were expressed in every plant organ examined
Nelissen H, Fleury D, Bruno L, Robles P, De Veylder L, Traas J, Micol JL, Van Montagu M, Inzé D, Van Lijsebettens M - The elongata mutants identify a functional Elongator complex in plants with a role in cell proliferation during organ growth
Visit the PubMed ArticleThe microarray experiment using mRNA of the shoot apices of the elo mutants showed that ... no difference in expression level was seen for the ELO3 gene between the elo3 mutant and Ler
Nelissen H, Fleury D, Bruno L, Robles P, De Veylder L, Traas J, Micol JL, Van Montagu M, Inzé D, Van Lijsebettens M - The elongata mutants identify a functional Elongator complex in plants with a role in cell proliferation during organ growth
Visit the PubMed ArticleThe expression of the ... ELO2 ... was monitored in different plant organs by RT-PCR: roots, young first leaves, whole seedlings (14 DAG), cotyledons, expanded first leaves, floral buds, and shoot apices (27 DAG ... were expressed in every plant organ examined
Nelissen H, Fleury D, Bruno L, Robles P, De Veylder L, Traas J, Micol JL, Van Montagu M, Inzé D, Van Lijsebettens M - The elongata mutants identify a functional Elongator complex in plants with a role in cell proliferation during organ growth
Visit the PubMed ArticleThe microarray experiment using mRNA of the shoot apices of the elo mutants showed that the expression of ELO2 in elo2 was only 20.1% that of the wild type
Nelissen H, Fleury D, Bruno L, Robles P, De Veylder L, Traas J, Micol JL, Van Montagu M, Inzé D, Van Lijsebettens M - The elongata mutants identify a functional Elongator complex in plants with a role in cell proliferation during organ growth
Visit the PubMed ArticleThe expression of the ELO1 ... was monitored in different plant organs by RT-PCR: roots, young first leaves, whole seedlings (14 DAG), cotyledons, expanded first leaves, floral buds, and shoot apices (27 DAG ... were expressed in every plant organ examined ... in every
Nelissen H, Fleury D, Bruno L, Robles P, De Veylder L, Traas J, Micol JL, Van Montagu M, Inzé D, Van Lijsebettens M - The elongata mutants identify a functional Elongator complex in plants with a role in cell proliferation during organ growth
Visit the PubMed ArticleThe expression of the ACL5 gene was examined in wild-type and tkv plants by reverse transcription (RT)-PCR and was found to be similarly up-regulated in tkv mutant plants (Fig. 7
Clay NK, Nelson T - Arabidopsis thickvein mutation affects vein thickness and organ vascularization, and resides in a provascular cell-specific spermine synthase involved in vein definition and in polar auxin transport
Visit the PubMed ArticleThe procambial-specific expression pattern continues during primary root development (Figs. 8, E and G, 9I) and early leaf development after an initially broad expression domain in leaf primordia (Figs. 8C and 9, E–H
Clay NK, Nelson T - Arabidopsis thickvein mutation affects vein thickness and organ vascularization, and resides in a provascular cell-specific spermine synthase involved in vein definition and in polar auxin transport
Visit the PubMed ArticleThe procambial-specific expression pattern continues during primary root development (Figs. 8, E and G, 9I) and early leaf development after an initially broad expression domain in leaf primordia (Figs. 8C and 9, E–H
Clay NK, Nelson T - Arabidopsis thickvein mutation affects vein thickness and organ vascularization, and resides in a provascular cell-specific spermine synthase involved in vein definition and in polar auxin transport
Visit the PubMed ArticleCYCD3;1 expressions were examined by RNA gel blot analysis and were found to be unchanged in smpepi ... plants
Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf
Visit the PubMed ArticleSWP expression level was examined by RNA gel blot analysis and RT-PCR and was found to be ... normal in ... smp2-1
Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf
Visit the PubMed ArticleANT overexpression activated ectopic expression of CYCD3;1
Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf
Visit the PubMed ArticleRT-PCR results indicated that the transcripts of ... SMP1 ... were reduced in smp1epi homozygous plants
Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf
Visit the PubMed Articlefull-length SWP cDNA driven by the 35S CaMV promoter was introduced into smpepi mutant plants ... RT-PCR results confirmed that two of these restored lines expressed a wild-type level of fully spliced SWP transcript and more so than the line that exhibited the mutant phenotype
Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf
Visit the PubMed Articletransgenic copy of the SMP1 promoter driving the β-glucuronidase (GUS) reporter gene ... After germination, SMP1 mRNA expression was ... not in the SAM
Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf
Visit the PubMed Articletransgenic copy of the SMP1 promoter driving the β-glucuronidase (GUS) reporter gene ... SMP1 promoter activity was also found throughout very young leaf primordia (Figure 8J) and as the leaf matured, was turned off in a tip-to-base manner (Figure 8K). This pattern of GUS expression overlapped with the general pattern of cell proliferation and presaged the basipetal wave of cell maturation that would flow from leaf tip to leaf base
Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf
Visit the PubMed Articletransgenic copy of the SMP1 promoter driving the β-glucuronidase (GUS) reporter gene ... After germination, SMP1 mRNA expression was found throughout leaf primordia
Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf
Visit the PubMed ArticleSMP1 promoter activity was also found throughout very young leaf primordia
Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf
Visit the PubMed ArticleCDC2A expression ... was ... unchanged in ... SMP1-overexpressing plants
Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf
Visit the PubMed ArticleCDC2A expression also was found to be unchanged in ... SMP1-overexpressing plants
Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf
Visit the PubMed Articlefull-length SWP cDNA driven by the 35S CaMV promoter was introduced into smpepi mutant plants. Because of the moderate infertility of these plants, only four independent lines were obtained ... transcript levels of ... SMP2 were also increased in all three lines
Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf
Visit the PubMed ArticleSMP1 mRNA expression was found throughout early globular-staged embryos
Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf
Visit the PubMed ArticleSWP expression level was examined by RNA gel blot analysis and RT-PCR and was found to be undetectable in smpepi plants
Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf
Visit the PubMed ArticleCYCD3;1 expressions were examined by RNA gel blot analysis and were found to be unchanged in ... SMP1-overexpressing plants
Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf
Visit the PubMed ArticleCDC2A expression also was found to be unchanged in smpepi ... plants
Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf
Visit the PubMed Articlefull-length SWP cDNA driven by the 35S CaMV promoter was introduced into smpepi mutant plants. Because of the moderate infertility of these plants, only four independent lines were obtained ... transcript levels of SMP1 ... were also increased in all three lines
Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf
Visit the PubMed ArticleANT ... expressions were examined by RNA gel blot analysis and were found to be unchanged in ... SMP1-overexpressing plants
Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf
Visit the PubMed ArticleSWP expression level was examined by RNA gel blot analysis and RT-PCR and was found to be ... normal ... in smp1-1
Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf
Visit the PubMed Articletransgenic copy of the SMP1 promoter driving the β-glucuronidase (GUS) reporter gene ... bent cotyledon stage where SMP1 promoter activity increased in procambial cells
Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf
Visit the PubMed ArticleSWP expression level was examined by RNA gel blot analysis and RT-PCR and was found to be ... normal in SMP1-overexpressing plants
Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf
Visit the PubMed ArticleANT ... expressions were examined by RNA gel blot analysis and were found to be unchanged in smpepi ... plants
Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf
Visit the PubMed ArticleCDC2A expression ... was ... unchanged in smpepi ... plants
Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf
Visit the PubMed ArticleSMP1 mRNA ... expression intensified in the cotyledon primordia of heart-staged embryos
Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf
Visit the PubMed ArticleRT-PCR results indicated that the transcripts of ... SMP2 were reduced in smp1epi homozygous plants
Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf
Visit the PubMed ArticleSMP1 expression is associated with regions of cell proliferation in lateral organs
Clay NK, Nelson T - The recessive epigenetic swellmap mutation affects the expression of two step II splicing factors required for the transcription of the cell proliferation gene STRUWWELPETER and for the timing of cell cycle arrest in the Arabidopsis leaf
Visit the PubMed ArticleWe also generated transgenic plants expressing ProARF2:GUS in order to monitor ARF2 expression during development. GUS activity was detected in the peripheral zone of cotyledons ... in 3-day-old, light-grown transgenic seedlings
Okushima Y, Mitina I, Quach HL, Theologis A - AUXIN RESPONSE FACTOR 2 (ARF2): a pleiotropic developmental regulator
Visit the PubMed ArticleThe results shown in Figure 1(c) indicate that ARF2 expression in wild-type seedlings ... slightly induced by IAA treatment
Okushima Y, Mitina I, Quach HL, Theologis A - AUXIN RESPONSE FACTOR 2 (ARF2): a pleiotropic developmental regulator
Visit the PubMed ArticleWe also generated transgenic plants expressing ProARF2:GUS in order to monitor ARF2 expression during development ... little or no GUS expression was detected in cotyledons of etiolated seedlings
Okushima Y, Mitina I, Quach HL, Theologis A - AUXIN RESPONSE FACTOR 2 (ARF2): a pleiotropic developmental regulator
Visit the PubMed ArticlePrevious studies by Ulmasov et al. (1999a) have shown that ARF2 is expressed in all major plant organs including roots, rosette and caulin leaves, flowers and siliques. These expression characteristics were also confirmed by us using RT-PCR analysis (data not shown
Okushima Y, Mitina I, Quach HL, Theologis A - AUXIN RESPONSE FACTOR 2 (ARF2): a pleiotropic developmental regulator
Visit the PubMed ArticleAN3 ... expressed at detectable levels in the shoot tip
Horiguchi G, Kim GT, Tsukaya H - The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana
Visit the PubMed ArticleThe expression domains of AtGRF4 ... partially overlapped with the AN3 expression domain, mainly near the midvein
Horiguchi G, Kim GT, Tsukaya H - The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana
Visit the PubMed ArticleAtGRF5 ... undetectable in mature leaves
Horiguchi G, Kim GT, Tsukaya H - The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana
Visit the PubMed ArticleGUS activities in ... AtGRF9 reporter plants were clearly restricted to the lower half of the leaf primordium ... Consistent with this analysis the RT-PCR analysis showed that AN3, AtGRF5, and AtGRF9 are expressed at stronger levels in the lower half than in the upper half of the primordium
Horiguchi G, Kim GT, Tsukaya H - The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana
Visit the PubMed ArticleAN3 expression was undetectable in mature leaves
Horiguchi G, Kim GT, Tsukaya H - The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana
Visit the PubMed ArticleAN3 was strongly expressed in the basal region of leaf primordia of 5-day-old plants
Horiguchi G, Kim GT, Tsukaya H - The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana
Visit the PubMed ArticleAtGRF9 ... undetectable in mature leaves
Horiguchi G, Kim GT, Tsukaya H - The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana
Visit the PubMed ArticleGUS activities in ... AtGRF5 ... reporter plants ... were clearly restricted to the lower half of the leaf primordium ... Consistent with this analysis the RT-PCR analysis showed that AN3, AtGRF5, and AtGRF9 are expressed at stronger levels in the lower half than in the upper half of the primordium
Horiguchi G, Kim GT, Tsukaya H - The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana
Visit the PubMed ArticleThe expression domains of ... AtGRF6 partially overlapped with the AN3 expression domain, mainly near the midvein
Horiguchi G, Kim GT, Tsukaya H - The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana
Visit the PubMed ArticleAtGRF9 ... expressed uniformly in the primordium
Horiguchi G, Kim GT, Tsukaya H - The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana
Visit the PubMed ArticleAtGRF7 was strongly expressed in cells surrounding the vasculature
Horiguchi G, Kim GT, Tsukaya H - The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana
Visit the PubMed ArticleGUS activities in AN3 ... reporter plants ... were clearly restricted to the lower half of the leaf primordium ... Consistent with this analysis the RT-PCR analysis showed that AN3, AtGRF5, and AtGRF9 are expressed at stronger levels in the lower half than in the upper half of the primordium
Horiguchi G, Kim GT, Tsukaya H - The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana
Visit the PubMed ArticleAN3 was ... expressed ... weakly in the shoot apical meristem
Horiguchi G, Kim GT, Tsukaya H - The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana
Visit the PubMed ArticleAtGRF5 ... expressed uniformly in the primordium
Horiguchi G, Kim GT, Tsukaya H - The transcription factor AtGRF5 and the transcription coactivator AN3 regulate cell proliferation in leaf primordia of Arabidopsis thaliana
Visit the PubMed ArticleIn spite of the striking similarity of the leaf phenotypes of as and aba1 mutants, ectopic expression of the KNAT1 ... was not found, as tested by RT-PCR amplifications of RNA extracted from the leaves of aba1/aba1 plants (data not shown
Barrero JM, Piqueras P, González-Guzmán M, Serrano R, Rodríguez PL, Ponce MR, Micol JL - A mutational analysis of the ABA1 gene of Arabidopsis thaliana highlights the involvement of ABA in vegetative development
Visit the PubMed ArticleIn spite of the striking similarity of the leaf phenotypes of as and aba1 mutants, ectopic expression of ... STM ... was not found, as tested by RT-PCR amplifications of RNA extracted from the leaves of aba1/aba1 plants (data not shown
Barrero JM, Piqueras P, González-Guzmán M, Serrano R, Rodríguez PL, Ponce MR, Micol JL - A mutational analysis of the ABA1 gene of Arabidopsis thaliana highlights the involvement of ABA in vegetative development
Visit the PubMed ArticleIn spite of the striking similarity of the leaf phenotypes of as and aba1 mutants, ectopic expression of the ... KNAT2 ... was not found, as tested by RT-PCR amplifications of RNA extracted from the leaves of aba1/aba1 plants (data not shown
Barrero JM, Piqueras P, González-Guzmán M, Serrano R, Rodríguez PL, Ponce MR, Micol JL - A mutational analysis of the ABA1 gene of Arabidopsis thaliana highlights the involvement of ABA in vegetative development
Visit the PubMed ArticleOur examination of ANT expression by real time RT-PCR produced results that are in agreement with previous reports of ANT expression in ... seedlings
Nole-Wilson S, Tranby TL, Krizek BA - AINTEGUMENTA-like (AIL) genes are expressed in young tissues and may specify meristematic or division-competent states
Visit the PubMed ArticleOur examination of ANT expression by real time RT-PCR ... we also observed low expression in mature rosette leaves, which was not previously detected by RNA gel blot analysis (Klucher et al., 1996
Nole-Wilson S, Tranby TL, Krizek BA - AINTEGUMENTA-like (AIL) genes are expressed in young tissues and may specify meristematic or division-competent states
Visit the PubMed ArticleANT is expressed at higher levels in most tissues compared to other members of the AIL group
Nole-Wilson S, Tranby TL, Krizek BA - AINTEGUMENTA-like (AIL) genes are expressed in young tissues and may specify meristematic or division-competent states
Visit the PubMed Articlethe gene encoding ribosomal protein L10A (At2g27530) ... were induced in the ATAF2 KO line compared to the wild type in the non-wounded case only
Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis
Visit the PubMed ArticlePDF1.1 ... were induced in the KO, in both non-wounded and wounded (30 min) conditions
Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis
Visit the PubMed ArticleArabidopsis plants transformed with the ATAF2 promoter fused to the GUS reporter gene (PATAF2:GUS) confirmed the sqRT-PCR results, indicating that ATAF2 was expressed at varying levels in all plant tissues. ATAF2 was highly expressed in ... leaves
Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis
Visit the PubMed ArticleThe expression pattern in cotyledons and leaves was not homogeneous, but GUS staining was present only in particular cell types (Figure 3b). Microscopic examination indicated that ATAF2 was expressed mainly in cells surrounding the sub-stomatal cavity
Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis
Visit the PubMed ArticleWhen plants were placed into a high osmotic medium containing mannitol to induce water loss, ATAF2 was induced 2.6-fold
Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis
Visit the PubMed ArticleWound induction of ATAF2 was not blocked by mutations affecting ABA biosynthesis (aba1; Koornneef et al., 1982)
Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis
Visit the PubMed ArticleGST1 (At1g02930) ... were induced in the ATAF2 KO line compared to the wild type in the non-wounded case only
Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis
Visit the PubMed ArticleATAF2 ... In mature leaves, strong expression was observed in the hydathodes
Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis
Visit the PubMed ArticleTo confirm the GUS staining results, we measured the expression level of ATAF2 at the wound site (local), in the non-wounded tissue next to the wound site (adjacent), in non-wounded leaves of a wounded plant (systemic) and in leaves of a control non-wounded plant (NWP) using sqRT-PCR (Figure 4). ATAF2 was induced after 30 min in the local tissue (approximately 13× over leaves of non-wounded plants), to a lower extent in the adjacent tissue (approximately 4×) and less in systemic leaves (approximately 2×). This high level of wounding induction is transient, and 5 h after wounding, induction levels were markedly reduced (6×, 2× and 2× over leaves of non-wounded plants, respectively
Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis
Visit the PubMed ArticleWound induction of ATAF2 was not blocked by mutations affecting ... ABA response pathways ... abi2
Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis
Visit the PubMed ArticlePATAF2:GUS transgenic Arabidopsis plants showed a strong induction of GUS activity immediately around the damaged area of a wounded leaf
Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis
Visit the PubMed ArticleATAF2 expression was induced in leaves by the application of ... SA (6.5X)
Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis
Visit the PubMed ArticlePR1 (At2g14610) ... were induced in the ATAF2 KO line compared to the wild type in the non-wounded case only
Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis
Visit the PubMed ArticleArabidopsis plants transformed with the ATAF2 promoter fused to the GUS reporter gene (PATAF2:GUS) confirmed the sqRT-PCR results, indicating that ATAF2 was expressed at varying levels in all plant tissues. ATAF2 was highly expressed in cotyledons
Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis
Visit the PubMed ArticleHowever, plants kept in the dark for 5 h showed increased ATAF2 expression (2.1-fold; Figure 5a), indicating that light negatively influences ATAF2 expression
Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis
Visit the PubMed ArticleATAF2 expression ... in leaves ... ABA treatment did not have any effect
Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis
Visit the PubMed ArticleWhen the response of ATAF2 to sugar was tested by transferring plants to liquid MS medium in the presence or absence of 3% sucrose 4 days prior to hormone or stress treatments, ATAF2 expression was higher in all treatments in the absence of sugars. This was especially evident in the response to dark, where ATAF2 induction was 2.6 times higher in the absence than in the presence of sucrose
Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis
Visit the PubMed ArticleATAF2 expression was induced in leaves by the application of MeJA (7.4X)
Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis
Visit the PubMed ArticleATAF2 ... in older senescing cotyledons, expression is clearly restricted to stomatal guard cells
Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis
Visit the PubMed Articlethe H-protein promoter binding factor-2a (At3g47500) were induced in the ATAF2 KO line compared to the wild type in the non-wounded case only
Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis
Visit the PubMed ArticleExpression analysis using semi-quantitative real-time reverse transcription polymerase chain reaction (sqRT-PCR) indicated that ATAF2 was expressed in roots, leaves, seedlings and mature flowers (Figure 2). The expression was highest in roots and leaves, and lowest in flower buds and bolt stems
Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis
Visit the PubMed Articlea gene encoding a 33 kDa secretory protein-related protein (At5g48540) were induced in the KO, in both non-wounded and wounded (30 min) conditions
Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis
Visit the PubMed ArticlePDF1.2 ... were induced in the KO, in both non-wounded and wounded (30 min) conditions
Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis
Visit the PubMed ArticleATAF2 ... In mature leaves ... a low background level of expression was occasionally observed in the whole leaf and/or in the vascular tissue
Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis
Visit the PubMed ArticleWound induction of ATAF2 was not blocked by mutations affecting ... ABA response pathways ... abi1
Delessert C, Kazan K, Wilson IW, Van Der Straeten D, Manners J, Dennis ES, Dolferus R - The transcription factor ATAF2 represses the expression of pathogenesis-related genes in Arabidopsis
Visit the PubMed ArticleWe then compared expression of AP1, FUL, and SEP3 in mature rosette leaves of wild-type plants or those overexpressing FT (Figure 2B) or TFT (see Supplemental Figure 3B online). Plants were grown for 30 d under conditions that enhance curling (conditions A2 and A4 in Table 1, respectively). All three genes were highly misexpressed in the rosette leaves of both transgenic lines. Fold induction ranged between 5.4 (FUL) and 21 (SEP3) for Pro35S:FT plants grown under long days
Teper-Bamnolker P, Samach A - The flowering integrator FT regulates SEPALLATA3 and FRUITFULL accumulation in Arabidopsis leaves
Visit the PubMed ArticleIn 30-d-old rosette leaves, we detected a consistent 10-fold decrease in the expression of SEP3 and FUL in ft-2 mutants
Teper-Bamnolker P, Samach A - The flowering integrator FT regulates SEPALLATA3 and FRUITFULL accumulation in Arabidopsis leaves
Visit the PubMed ArticleWe therefore asked whether FD is required for FT-dependent accumulation of SEP3 and FUL in mature rosette leaves. Indeed, the fd-1 mutation had an effect similar to that of ft-2 in reducing the expression of these genes in mature rosette leaves (Figure 2C
Teper-Bamnolker P, Samach A - The flowering integrator FT regulates SEPALLATA3 and FRUITFULL accumulation in Arabidopsis leaves
Visit the PubMed ArticleWe then compared expression of AP1, FUL, and SEP3 in mature rosette leaves of wild-type plants or those overexpressing FT (Figure 2B) or TFT (see Supplemental Figure 3B online). Plants were grown for 30 d under conditions that enhance curling (conditions A2 and A4 in Table 1, respectively). All three genes were highly misexpressed in the rosette leaves of both transgenic lines. Fold induction ranged between 5.4 (FUL) and 21 (SEP3) for Pro35S:FT plants grown under long days
Teper-Bamnolker P, Samach A - The flowering integrator FT regulates SEPALLATA3 and FRUITFULL accumulation in Arabidopsis leaves
Visit the PubMed ArticleIn 30-d-old rosette leaves, we detected a consistent 10-fold decrease in the expression of SEP3 and FUL in ft-2 mutants
Teper-Bamnolker P, Samach A - The flowering integrator FT regulates SEPALLATA3 and FRUITFULL accumulation in Arabidopsis leaves
Visit the PubMed ArticleWe then compared expression of AP1, FUL, and SEP3 in mature rosette leaves of wild-type plants or those overexpressing FT (Figure 2B) or TFT (see Supplemental Figure 3B online). Plants were grown for 30 d under conditions that enhance curling (conditions A2 and A4 in Table 1, respectively). All three genes were highly misexpressed in the rosette leaves of both transgenic lines. Fold induction ranged between 5.4 (FUL) and 21 (SEP3) for Pro35S:FT plants grown under long days
Teper-Bamnolker P, Samach A - The flowering integrator FT regulates SEPALLATA3 and FRUITFULL accumulation in Arabidopsis leaves
Visit the PubMed ArticleWe therefore asked whether FD is required for FT-dependent accumulation of SEP3 and FUL in mature rosette leaves. Indeed, the fd-1 mutation had an effect similar to that of ft-2 in reducing the expression of these genes in mature rosette leaves (Figure 2C
Teper-Bamnolker P, Samach A - The flowering integrator FT regulates SEPALLATA3 and FRUITFULL accumulation in Arabidopsis leaves
Visit the PubMed ArticleThe expression of AS2 was not altered in the ... GTE6 RNAi plants
Chua YL, Channelière S, Mott E, Gray JC - The bromodomain protein GTE6 controls leaf development in Arabidopsis by histone acetylation at ASYMMETRIC LEAVES1
Visit the PubMed ArticleThe expression of AS2 was not altered in the GTE6 overexpressing mutants
Chua YL, Channelière S, Mott E, Gray JC - The bromodomain protein GTE6 controls leaf development in Arabidopsis by histone acetylation at ASYMMETRIC LEAVES1
Visit the PubMed ArticleThe expression of other genes involved in leaf development, such as ... TPC2 ... was also investigated. However, no change in the transcript levels of these genes was observed in the GTE6 mutants, suggesting that GTE6 is not regulating the expression of these genes
Chua YL, Channelière S, Mott E, Gray JC - The bromodomain protein GTE6 controls leaf development in Arabidopsis by histone acetylation at ASYMMETRIC LEAVES1
Visit the PubMed ArticleThe expression of other genes involved in leaf development, such as ... CLF ... was also investigated. However, no change in the transcript levels of these genes was observed in the GTE6 mutants, suggesting that GTE6 is not regulating the expression of these genes
Chua YL, Channelière S, Mott E, Gray JC - The bromodomain protein GTE6 controls leaf development in Arabidopsis by histone acetylation at ASYMMETRIC LEAVES1
Visit the PubMed ArticleElevated GTE6 expression in the 35S::GTE6 plants resulted in more AS1 transcripts
Chua YL, Channelière S, Mott E, Gray JC - The bromodomain protein GTE6 controls leaf development in Arabidopsis by histone acetylation at ASYMMETRIC LEAVES1
Visit the PubMed ArticleThe expression of other genes involved in leaf development, such as ... DRL ... was also investigated. However, no change in the transcript levels of these genes was observed in the GTE6 mutants, suggesting that GTE6 is not regulating the expression of these genes
Chua YL, Channelière S, Mott E, Gray JC - The bromodomain protein GTE6 controls leaf development in Arabidopsis by histone acetylation at ASYMMETRIC LEAVES1
Visit the PubMed ArticleIn the basal rosette leaves of 21-d-old wild-type plants, GTE6 transcripts are more abundant in leaves 6 and 7, which possess narrow elliptical laminae, than in leaves 1-4, which have round laminae (Fig. 1C,D), suggesting a possible correlation between GTE6 expression and the formation of elliptical leaf laminae in mature leaves
Chua YL, Channelière S, Mott E, Gray JC - The bromodomain protein GTE6 controls leaf development in Arabidopsis by histone acetylation at ASYMMETRIC LEAVES1
Visit the PubMed ArticleThe expression of other genes involved in leaf development, such as ... ROT3 ... was also investigated. However, no change in the transcript levels of these genes was observed in the GTE6 mutants, suggesting that GTE6 is not regulating the expression of these genes
Chua YL, Channelière S, Mott E, Gray JC - The bromodomain protein GTE6 controls leaf development in Arabidopsis by histone acetylation at ASYMMETRIC LEAVES1
Visit the PubMed ArticleThe T-DNA insertions resulted in fewer GTE6 transcripts in leaf 7 (Fig. 2B), suggesting misregulation of GTE6 expression. The transcript abundance of GTE6 in leaf 7 of the insertion mutants was similar to leaf 4 of the wild-type and the insertion mutant plants, suggesting that the T-DNA insertions have disrupted the activation of GTE6 expression in the mature leaves ... Thus, elevated expression of GTE6 in leaves 6 and 7 of wild-type plants is important for the development of the elliptical leaf lamina
Chua YL, Channelière S, Mott E, Gray JC - The bromodomain protein GTE6 controls leaf development in Arabidopsis by histone acetylation at ASYMMETRIC LEAVES1
Visit the PubMed ArticleThe expression of other genes involved in leaf development, such as AN ... was also investigated. However, no change in the transcript levels of these genes was observed in the GTE6 mutants, suggesting that GTE6 is not regulating the expression of these genes
Chua YL, Channelière S, Mott E, Gray JC - The bromodomain protein GTE6 controls leaf development in Arabidopsis by histone acetylation at ASYMMETRIC LEAVES1
Visit the PubMed Articledecreased expression of GTE6 in the RNAi plants resulted in fewer AS1 transcripts
Chua YL, Channelière S, Mott E, Gray JC - The bromodomain protein GTE6 controls leaf development in Arabidopsis by histone acetylation at ASYMMETRIC LEAVES1
Visit the PubMed ArticleIn wild-type plants, PIAA3::GUS was expressed in ... cotyledons
Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana
Visit the PubMed ArticleARF19 ... induced in senescing leaves (Lin and Wu, 2004) and this was confirmed by RNA gel blot analysis (Fig. 8C
Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana
Visit the PubMed ArticleEctopic PIAA3::GUS ... stain was also observed in the leaves of arf1-4 seedlings
Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana
Visit the PubMed ArticleIAA5 mRNA levels were increased in auxin-treated arf1-4 seedlings
Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana
Visit the PubMed ArticleRNA levels of ... IAA5 ... in arf2 flowers (Fig. 6S) and seedlings (data not shown) were similar to those of Columbia plants
Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana
Visit the PubMed ArticleIAA2 ... mRNA levels were increased in auxin-treated arf1-4 seedlings
Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana
Visit the PubMed ArticleIn wild-type plants ... PIAA7::GUS was expressed in ... shoot meristems
Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana
Visit the PubMed ArticlePARF1::GUS ... X-Gluc staining appeared throughout 8-day-old seedlings and in rosette leaves (data not shown
Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana
Visit the PubMed ArticleMicroarray studies also indicated that ARF2 mRNA increased during senescence (Lin and Wu, 2004). We examined PARF2::GUS::ARF2 plants (Li et al., 2004) to determine the levels of ARF2 protein in senescing leaves. In mature, fresh fully expanded rosette leaves or in those placed in the dark for 2 days, little GUS staining was observed (Fig. 4A,B). After 4 and 6 days in darkness, GUS staining was evident (Fig. 4C,D). GUS activity was highest in vasculature, but was also present in ground tissue. Together these data indicate that ARF2 accumulated during leaf senescence
Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana
Visit the PubMed ArticleRNA gel blot hybridizations confirmed that endogenous ... IAA7 mRNA levels were increased in arf1-4 flowers and seedlings, and in arf1-4 arf2-8 flowers (Fig. 6S
Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana
Visit the PubMed ArticleEctopic ... PIAA7::GUS stain was also observed in the leaves of arf1-4 seedlings
Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana
Visit the PubMed ArticleARF1 mRNA decreased as senescence progressed in ... Columbia ... plants
Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana
Visit the PubMed ArticleARF1 mRNA decreased as senescence progressed in ... arf2-8 plants
Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana
Visit the PubMed ArticleIn wild-type plants, PIAA3::GUS was expressed in ... expanding leaves
Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana
Visit the PubMed ArticlePARF2::GUS ... X-Gluc staining appeared throughout 8-day-old seedlings and in rosette leaves (data not shown
Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana
Visit the PubMed ArticleRNA levels of ... IAA7 ... in arf2 flowers (Fig. 6S) and seedlings (data not shown) were similar to those of Columbia plants
Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana
Visit the PubMed ArticleNPH4/ARF7 mRNA levels in arf2-8 leaves were approximately twofold higher than in Columbia leaves at the start of the experiment
Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana
Visit the PubMed ArticleRNA gel blot hybridizations confirmed that endogenous IAA3 ... mRNA levels were increased in arf1-4 flowers and seedlings, and in arf1-4 arf2-8 flowers (Fig. 6S
Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana
Visit the PubMed ArticleRNA levels of IAA2 ... in arf2 flowers (Fig. 6S) and seedlings (data not shown) were similar to those of Columbia plants
Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana
Visit the PubMed ArticleARF1 ... present in roots, rosette leaves, cauline leaves and flowers (Ulmasov et al., 1999b
Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana
Visit the PubMed ArticleYounger, expanding leaves of PARF2::GUS::ARF2 plants also stained in the vasculature and ground tissue, both in freshly detached leaves and in those placed in the dark for 4 days
Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana
Visit the PubMed ArticleARF2 mRNA ... present in roots, rosette leaves, cauline leaves and flowers (Ulmasov et al., 1999b
Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana
Visit the PubMed ArticleRNA levels of ... IAA3 ... in arf2 flowers (Fig. 6S) and seedlings (data not shown) were similar to those of Columbia plants
Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana
Visit the PubMed ArticleNPH4/ARF7 ... induced in senescing leaves (Lin and Wu, 2004) and this was confirmed by RNA gel blot analysis (Fig. 8C
Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana
Visit the PubMed ArticleYounger, expanding leaves of PARF2::GUS::ARF2 plants also stained in the vasculature ... both in freshly detached leaves and in those placed in the dark for 4 days
Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana
Visit the PubMed ArticleARF1 mRNA levels were quantified in three independent blots and were found to be slightly higher (2.7±0.9-fold) in freshly detached arf2 leaves when compared with Columbia leaves
Ellis CM, Nagpal P, Young JC, Hagen G, Guilfoyle TJ, Reed JW - AUXIN RESPONSE FACTOR1 and AUXIN RESPONSE FACTOR2 regulate senescence and floral organ abscission in Arabidopsis thaliana
Visit the PubMed ArticleZip III gene CORONA (CNA)17 ... shows elevated expression in se-3 plants
Grigg SP, Canales C, Hay A, Tsiantis M - SERRATE coordinates shoot meristem function and leaf axial patterning in Arabidopsis
Visit the PubMed ArticleMisexpression of PHB in se mutants was evident at the eight-cell stage of embryogenesis
Grigg SP, Canales C, Hay A, Tsiantis M - SERRATE coordinates shoot meristem function and leaf axial patterning in Arabidopsis
Visit the PubMed ArticlePHB ... in se plants it is expressed broadly, extending into the abaxial ... domain
Grigg SP, Canales C, Hay A, Tsiantis M - SERRATE coordinates shoot meristem function and leaf axial patterning in Arabidopsis
Visit the PubMed ArticleBP ... are not ... misregulated in se mutants
Grigg SP, Canales C, Hay A, Tsiantis M - SERRATE coordinates shoot meristem function and leaf axial patterning in Arabidopsis
Visit the PubMed Articlese-3 plants with hypertrophic apices express PHB transcript strongly ... lateral organ primordia
Grigg SP, Canales C, Hay A, Tsiantis M - SERRATE coordinates shoot meristem function and leaf axial patterning in Arabidopsis
Visit the PubMed ArticleLevels of miR165 ... primary transcripts were elevated in se mutants compared to wild type
Grigg SP, Canales C, Hay A, Tsiantis M - SERRATE coordinates shoot meristem function and leaf axial patterning in Arabidopsis
Visit the PubMed ArticleLevels of ... miR166 primary transcripts were elevated in se mutants compared to wild type
Grigg SP, Canales C, Hay A, Tsiantis M - SERRATE coordinates shoot meristem function and leaf axial patterning in Arabidopsis
Visit the PubMed ArticleKNAT2 are not ... misregulated in se mutants
Grigg SP, Canales C, Hay A, Tsiantis M - SERRATE coordinates shoot meristem function and leaf axial patterning in Arabidopsis
Visit the PubMed ArticlePHB expression domain is expanded in se mutant SAMs
Grigg SP, Canales C, Hay A, Tsiantis M - SERRATE coordinates shoot meristem function and leaf axial patterning in Arabidopsis
Visit the PubMed ArticleAll three se alleles show increased expression of ... PHB
Grigg SP, Canales C, Hay A, Tsiantis M - SERRATE coordinates shoot meristem function and leaf axial patterning in Arabidopsis
Visit the PubMed ArticleSTM ... are not ... misregulated in se mutants
Grigg SP, Canales C, Hay A, Tsiantis M - SERRATE coordinates shoot meristem function and leaf axial patterning in Arabidopsis
Visit the PubMed Articlein arf2 mutants ... we detected ... CYCD3;1 ... expression in mature leaves of mutant but not wild-type plants, indicating that loss of ARF2 function prolongs expression of these genes
Schruff MC, Spielman M, Tiwari S, Adams S, Fenby N, Scott RJ - The AUXIN RESPONSE FACTOR 2 gene of Arabidopsis links auxin signalling, cell division, and the size of seeds and other organs
Visit the PubMed Articlein arf2 mutants ... ARGOS was expressed in mature leaf and stem at similar levels in mutants and wild type
Schruff MC, Spielman M, Tiwari S, Adams S, Fenby N, Scott RJ - The AUXIN RESPONSE FACTOR 2 gene of Arabidopsis links auxin signalling, cell division, and the size of seeds and other organs
Visit the PubMed ArticleRNA gel blot analysis has shown that ARF2 is expressed in roots, leaves, flowers and siliques (Ulmasov et al., 1999b
Schruff MC, Spielman M, Tiwari S, Adams S, Fenby N, Scott RJ - The AUXIN RESPONSE FACTOR 2 gene of Arabidopsis links auxin signalling, cell division, and the size of seeds and other organs
Visit the PubMed Articlein arf2 mutants ... we detected both ANT ... expression in mature leaves of mutant but not wild-type plants
Schruff MC, Spielman M, Tiwari S, Adams S, Fenby N, Scott RJ - The AUXIN RESPONSE FACTOR 2 gene of Arabidopsis links auxin signalling, cell division, and the size of seeds and other organs
Visit the PubMed ArticleWe found that hyp7 has similar GL2::GUS expression patterns in leaf trichomes
Sugimoto-Shirasu K, Roberts GR, Stacey NJ, McCann MC, Maxwell A, Roberts K - RHL1 is an essential component of the plant DNA topoisomerase VI complex and is required for ploidy-dependent cell growth
Visit the PubMed Articleall topo VI genes, AtSPO11-3/RHL2, AtTOP6B/HYP6/RHL3, and RHL1, are expressed in proliferating and endoreduplicating cells
Sugimoto-Shirasu K, Roberts GR, Stacey NJ, McCann MC, Maxwell A, Roberts K - RHL1 is an essential component of the plant DNA topoisomerase VI complex and is required for ploidy-dependent cell growth
Visit the PubMed Articlewe found that the topo II gene, AtTOP2, is preferentially expressed in proliferating cells
Sugimoto-Shirasu K, Roberts GR, Stacey NJ, McCann MC, Maxwell A, Roberts K - RHL1 is an essential component of the plant DNA topoisomerase VI complex and is required for ploidy-dependent cell growth
Visit the PubMed Articleall topo VI genes, AtSPO11-3/RHL2, AtTOP6B/HYP6/RHL3, and RHL1, are expressed in proliferating and endoreduplicating cells
Sugimoto-Shirasu K, Roberts GR, Stacey NJ, McCann MC, Maxwell A, Roberts K - RHL1 is an essential component of the plant DNA topoisomerase VI complex and is required for ploidy-dependent cell growth
Visit the PubMed Articlein vivo visualization revealed that the AtSPO11–3/RHL2 protein fused to GFP is present in ... shoot apical meristem
Sugimoto-Shirasu K, Roberts GR, Stacey NJ, McCann MC, Maxwell A, Roberts K - RHL1 is an essential component of the plant DNA topoisomerase VI complex and is required for ploidy-dependent cell growth
Visit the PubMed Articleall topo VI genes, AtSPO11-3/RHL2, AtTOP6B/HYP6/RHL3, and RHL1, are expressed in proliferating and endoreduplicating cells
Sugimoto-Shirasu K, Roberts GR, Stacey NJ, McCann MC, Maxwell A, Roberts K - RHL1 is an essential component of the plant DNA topoisomerase VI complex and is required for ploidy-dependent cell growth
Visit the PubMed Articlein vivo visualization revealed that the AtSPO11–3/RHL2 protein fused to GFP is present in ... young leaf petiole cells
Sugimoto-Shirasu K, Roberts GR, Stacey NJ, McCann MC, Maxwell A, Roberts K - RHL1 is an essential component of the plant DNA topoisomerase VI complex and is required for ploidy-dependent cell growth
Visit the PubMed Articledexamethasone (Dex)-inducible system ... Arabidopsis transgenic plants that can express geminivirus RepA protein (RepAwt) in an inducible manner ... we detected the viral protein by immunofluorescence in cross sections of leaves. As shown in Figure 1F, RepA accumulates in all leaf cell layers after Dex treatment
Desvoyes B, Ramirez-Parra E, Xie Q, Chua NH, Gutierrez C - Cell type-specific role of the retinoblastoma/E2F pathway during Arabidopsis leaf development
Visit the PubMed Articledexamethasone (Dex)-inducible system ... Arabidopsis transgenic plants that can express geminivirus RepA protein (RepAwt) in an inducible manner ... PCNA ... expression ... increased in the RepAwt-expressing plants
Desvoyes B, Ramirez-Parra E, Xie Q, Chua NH, Gutierrez C - Cell type-specific role of the retinoblastoma/E2F pathway during Arabidopsis leaf development
Visit the PubMed Articledexamethasone (Dex)-inducible system ... Arabidopsis transgenic plants that can express geminivirus RepA protein (RepAwt) in an inducible manner ... we determined by real-time reverse transcription (RT)-PCR the mRNA levels of ... after induction of the viral protein ... E2Fc ... was up-regulated in these plants
Desvoyes B, Ramirez-Parra E, Xie Q, Chua NH, Gutierrez C - Cell type-specific role of the retinoblastoma/E2F pathway during Arabidopsis leaf development
Visit the PubMed Articledexamethasone (Dex)-inducible system ... Arabidopsis transgenic plants that can express geminivirus RepA protein (RepAwt) in an inducible manner ... CDC6 ... expression ... increased in the RepAwt-expressing plants
Desvoyes B, Ramirez-Parra E, Xie Q, Chua NH, Gutierrez C - Cell type-specific role of the retinoblastoma/E2F pathway during Arabidopsis leaf development
Visit the PubMed Articledexamethasone (Dex)-inducible system ... Arabidopsis transgenic plants that can express geminivirus RepA protein (RepAwt) in an inducible manner ... expressing a translational fusion of the β-glucuronidase (GUS) reporter gene with the destruction box of cyclin B1;1, a useful G2/M marker ... after induction by Dex, we observed a stronger GUS expression in the leaf primordia
Desvoyes B, Ramirez-Parra E, Xie Q, Chua NH, Gutierrez C - Cell type-specific role of the retinoblastoma/E2F pathway during Arabidopsis leaf development
Visit the PubMed Articledexamethasone (Dex)-inducible system ... Arabidopsis transgenic plants that can express geminivirus RepA protein (RepAwt) in an inducible manner ... CDT1 ... expression ... increased in the RepAwt-expressing plants
Desvoyes B, Ramirez-Parra E, Xie Q, Chua NH, Gutierrez C - Cell type-specific role of the retinoblastoma/E2F pathway during Arabidopsis leaf development
Visit the PubMed Articledexamethasone (Dex)-inducible system ... Arabidopsis transgenic plants that can express geminivirus RepA protein (RepAwt) in an inducible manner ... we determined by real-time reverse transcription (RT)-PCR the mRNA levels of ... after induction of the viral protein ... E2Fa ... was up-regulated in these plants
Desvoyes B, Ramirez-Parra E, Xie Q, Chua NH, Gutierrez C - Cell type-specific role of the retinoblastoma/E2F pathway during Arabidopsis leaf development
Visit the PubMed Articledexamethasone (Dex)-inducible system ... Arabidopsis transgenic plants that can express geminivirus RepA protein (RepAwt) in an inducible manner ... expressing a translational fusion of the β-glucuronidase (GUS) reporter gene with the destruction box of cyclin B1;1, a useful G2/M marker ... after induction by Dex, we observed a stronger GUS expression ... in the proximal zone of young leaves
Desvoyes B, Ramirez-Parra E, Xie Q, Chua NH, Gutierrez C - Cell type-specific role of the retinoblastoma/E2F pathway during Arabidopsis leaf development
Visit the PubMed Articledexamethasone (Dex)-inducible system ... Arabidopsis transgenic plants that can express geminivirus RepA protein (RepAwt) in an inducible manner ... members of the ORC (origin recognition complex ... expression ... increased in the RepAwt-expressing plants
Desvoyes B, Ramirez-Parra E, Xie Q, Chua NH, Gutierrez C - Cell type-specific role of the retinoblastoma/E2F pathway during Arabidopsis leaf development
Visit the PubMed ArticleBAM1, BAM2 and BAM3 were broadly expressed throughout the different tissues and developmental stages examined
DeYoung BJ, Bickle KL, Schrage KJ, Muskett P, Patel K, Clark SE - The CLAVATA1-related BAM1, BAM2 and BAM3 receptor kinase-like proteins are required for meristem function in Arabidopsis
Visit the PubMed ArticleA robust signal for both BAM1 and BAM2 genes could be detected on the flanks of the meristem (Figure 5)
DeYoung BJ, Bickle KL, Schrage KJ, Muskett P, Patel K, Clark SE - The CLAVATA1-related BAM1, BAM2 and BAM3 receptor kinase-like proteins are required for meristem function in Arabidopsis
Visit the PubMed ArticleBAM1, BAM2 and BAM3 were broadly expressed throughout the different tissues and developmental stages examined
DeYoung BJ, Bickle KL, Schrage KJ, Muskett P, Patel K, Clark SE - The CLAVATA1-related BAM1, BAM2 and BAM3 receptor kinase-like proteins are required for meristem function in Arabidopsis
Visit the PubMed ArticleBAM1, BAM2 and BAM3 were broadly expressed throughout the different tissues and developmental stages examined
DeYoung BJ, Bickle KL, Schrage KJ, Muskett P, Patel K, Clark SE - The CLAVATA1-related BAM1, BAM2 and BAM3 receptor kinase-like proteins are required for meristem function in Arabidopsis
Visit the PubMed ArticleA robust signal for both BAM1 and BAM2 genes could be detected on the flanks of the meristem (Figure 5
DeYoung BJ, Bickle KL, Schrage KJ, Muskett P, Patel K, Clark SE - The CLAVATA1-related BAM1, BAM2 and BAM3 receptor kinase-like proteins are required for meristem function in Arabidopsis
Visit the PubMed ArticleGenes expressed to higher levels in the cyp98A3 mutant were clearly over-represented in the functional group related to jasmonate signaling (Fig. 8B). Indeed, many genes involved in the biosynthesis of jasmonate are significantly higher expressed in the mutant compared to wild type (Ws); These include LOX2 (see above), LOX1, allene oxide synthase (CYP74A, At5g42650), and allene oxide cyclase 4 (At1g13280). In addition, genes related to the abscisic acid cascade are over-represented in the group of genes expressed to higher levels in cyp98A3 insertion mutants (Fig. 8B). These include the gene responsive to dehydration 22 (RD22I, At5g25610), which is strongly induced by abscisic acid and drought (Yamaguchi-Shinozaki and Shinozaki, 1993), and the Arg decarboxylase 2 (ADC2, At4g34710) gene, which is strongly activated by abscisic acid, but also by methyl jasmonate and mechanical wounding, and is involved in polyamine biosynthesis (Perez-Amador et al., 2002). Also expressed to higher levels in cyp98A3 insertion mutants were two homeodomain Leu-zipper transcription factors (ATHB5, At5g65310; and ATHB7, At2g46680) involved in abscisic acid-mediated signaling pathways related to drought and osmotic stress (Söderman et al., 1996) and seed germination (Johannesson et al., 2003), respectively
Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth
Visit the PubMed ArticleIndeed, several genes encoding proteins involved in the light-harvesting complex (e.g. chlorophyll a/b-binding proteins At2g34430, At3g08940, and At3g47470), in photosynthetic electron transport (e.g. the putative ferredoxin At1g10960 and proton gradient regulation 5, At2g05620; Munekage et al., 2002), and in the Calvin cycle (e.g. Rubisco At5g38410 and the putative Rib-5-P isomerase At3g04790) are strongly down-regulated in the mutant compared to the wild type (Supplemental Table I
Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth
Visit the PubMed ArticleIn contrast, genes expressed to higher levels in cyp98A3 insertion mutants compared to wild type were over-represented in functional categories covering carbohydrate metabolism and include genes encoding enzymes in glycolysis (e.g. the glyceraldehyde 3-P dehydrogenase, At3g04120), the tricarboxylic acid cycle (e.g. the phosphoenolpyruvate carboxylase, At3g14940), and other anaerobic metabolisms (alcohol dehydrogenase, At1g77120). Also, functional categories related to defense responses were over-represented (Fig. 8A). Among these were genes known to be transcriptionally induced by insect feeding (the β-glucosidase BGL1, At1g52400; and the lipoxygenase LOX2, At3g45140; Stotz et al., 2000) or wounding/jasmonate (e.g. jasmonate response JR1, At3g16470; Leon et al., 1998). Finally, genes involved in secondary metabolism are over-represented in the group of up-regulated genes (Fig. 8A). It is noteworthy that genes encoding enzymes involved in flavonoid and anthocyanin biosynthesis and transport are expressed to higher levels in the cyp98A3 insertion mutant; These include chalcone isomerase (CHI, At3g55120), dihydroflavonol 4-reductase (DFR, At5g42800), flavanone 3-hydroxylase (F3H, At3g51240), and gluthation transferase (TT19, At5g17220; Weisshaar and Jenkins, 1998; Kitamura et al., 2004
Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth
Visit the PubMed ArticleWhile transcript levels of genes encoding Phe ammonia lyase (PAL1; At2g37040) and HCT (At5g48930; Fig. 7A) were comparable in cyp98A3 mutants and wild type at all time points, an increase in C4H (CYP73A5; At2g30490) expression compared to wild type was observed at the 2-, 4-, and 8-h time points in cyp98A3 insertion mutants (Fig. 7A
Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth
Visit the PubMed ArticleGenes expressed to higher levels in the cyp98A3 mutant were clearly over-represented in the functional group related to jasmonate signaling (Fig. 8B). Indeed, many genes involved in the biosynthesis of jasmonate are significantly higher expressed in the mutant compared to wild type (Ws); These include LOX2 (see above), LOX1, allene oxide synthase (CYP74A, At5g42650), and allene oxide cyclase 4 (At1g13280). In addition, genes related to the abscisic acid cascade are over-represented in the group of genes expressed to higher levels in cyp98A3 insertion mutants (Fig. 8B). These include the gene responsive to dehydration 22 (RD22I, At5g25610), which is strongly induced by abscisic acid and drought (Yamaguchi-Shinozaki and Shinozaki, 1993), and the Arg decarboxylase 2 (ADC2, At4g34710) gene, which is strongly activated by abscisic acid, but also by methyl jasmonate and mechanical wounding, and is involved in polyamine biosynthesis (Perez-Amador et al., 2002). Also expressed to higher levels in cyp98A3 insertion mutants were two homeodomain Leu-zipper transcription factors (ATHB5, At5g65310; and ATHB7, At2g46680) involved in abscisic acid-mediated signaling pathways related to drought and osmotic stress (Söderman et al., 1996) and seed germination (Johannesson et al., 2003), respectively
Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth
Visit the PubMed ArticleGenes expressed to higher levels in the cyp98A3 mutant were clearly over-represented in the functional group related to jasmonate signaling (Fig. 8B). Indeed, many genes involved in the biosynthesis of jasmonate are significantly higher expressed in the mutant compared to wild type (Ws); These include LOX2 (see above), LOX1, allene oxide synthase (CYP74A, At5g42650), and allene oxide cyclase 4 (At1g13280). In addition, genes related to the abscisic acid cascade are over-represented in the group of genes expressed to higher levels in cyp98A3 insertion mutants (Fig. 8B). These include the gene responsive to dehydration 22 (RD22I, At5g25610), which is strongly induced by abscisic acid and drought (Yamaguchi-Shinozaki and Shinozaki, 1993), and the Arg decarboxylase 2 (ADC2, At4g34710) gene, which is strongly activated by abscisic acid, but also by methyl jasmonate and mechanical wounding, and is involved in polyamine biosynthesis (Perez-Amador et al., 2002). Also expressed to higher levels in cyp98A3 insertion mutants were two homeodomain Leu-zipper transcription factors (ATHB5, At5g65310; and ATHB7, At2g46680) involved in abscisic acid-mediated signaling pathways related to drought and osmotic stress (Söderman et al., 1996) and seed germination (Johannesson et al., 2003), respectively
Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth
Visit the PubMed ArticleIndeed, several genes encoding proteins involved in the light-harvesting complex (e.g. chlorophyll a/b-binding proteins At2g34430, At3g08940, and At3g47470), in photosynthetic electron transport (e.g. the putative ferredoxin At1g10960 and proton gradient regulation 5, At2g05620; Munekage et al., 2002), and in the Calvin cycle (e.g. Rubisco At5g38410 and the putative Rib-5-P isomerase At3g04790) are strongly down-regulated in the mutant compared to the wild type (Supplemental Table I
Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth
Visit the PubMed ArticleIndeed, several genes encoding proteins involved in the light-harvesting complex (e.g. chlorophyll a/b-binding proteins At2g34430, At3g08940, and At3g47470), in photosynthetic electron transport (e.g. the putative ferredoxin At1g10960 and proton gradient regulation 5, At2g05620; Munekage et al., 2002), and in the Calvin cycle (e.g. Rubisco At5g38410 and the putative Rib-5-P isomerase At3g04790) are strongly down-regulated in the mutant compared to the wild type (Supplemental Table I
Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth
Visit the PubMed ArticleSix of the 28 expansin family members represented on the array used are differentially expressed, significantly more than expected by chance. However, some expansin isoforms (EXP1, At1g69530; EXP10, At1g26770; and EXPR, At4g17030) are expressed to higher levels in cyp98A3 insertion mutants, whereas others (EXP8, At2g40610; EXP3, At2g37640; and EXP11, At1g20190) are down-regulated compared to levels found in wild-type plants
Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth
Visit the PubMed ArticleIn contrast, genes expressed to higher levels in cyp98A3 insertion mutants compared to wild type were over-represented in functional categories covering carbohydrate metabolism and include genes encoding enzymes in glycolysis (e.g. the glyceraldehyde 3-P dehydrogenase, At3g04120), the tricarboxylic acid cycle (e.g. the phosphoenolpyruvate carboxylase, At3g14940), and other anaerobic metabolisms (alcohol dehydrogenase, At1g77120). Also, functional categories related to defense responses were over-represented (Fig. 8A). Among these were genes known to be transcriptionally induced by insect feeding (the β-glucosidase BGL1, At1g52400; and the lipoxygenase LOX2, At3g45140; Stotz et al., 2000) or wounding/jasmonate (e.g. jasmonate response JR1, At3g16470; Leon et al., 1998). Finally, genes involved in secondary metabolism are over-represented in the group of up-regulated genes (Fig. 8A). It is noteworthy that genes encoding enzymes involved in flavonoid and anthocyanin biosynthesis and transport are expressed to higher levels in the cyp98A3 insertion mutant; These include chalcone isomerase (CHI, At3g55120), dihydroflavonol 4-reductase (DFR, At5g42800), flavanone 3-hydroxylase (F3H, At3g51240), and gluthation transferase (TT19, At5g17220; Weisshaar and Jenkins, 1998; Kitamura et al., 2004
Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth
Visit the PubMed ArticleIndeed, several genes encoding proteins involved in the light-harvesting complex (e.g. chlorophyll a/b-binding proteins At2g34430, At3g08940, and At3g47470), in photosynthetic electron transport (e.g. the putative ferredoxin At1g10960 and proton gradient regulation 5, At2g05620; Munekage et al., 2002), and in the Calvin cycle (e.g. Rubisco At5g38410 and the putative Rib-5-P isomerase At3g04790) are strongly down-regulated in the mutant compared to the wild type (Supplemental Table I
Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth
Visit the PubMed ArticleIn contrast, genes expressed to higher levels in cyp98A3 insertion mutants compared to wild type were over-represented in functional categories covering carbohydrate metabolism and include genes encoding enzymes in glycolysis (e.g. the glyceraldehyde 3-P dehydrogenase, At3g04120), the tricarboxylic acid cycle (e.g. the phosphoenolpyruvate carboxylase, At3g14940), and other anaerobic metabolisms (alcohol dehydrogenase, At1g77120). Also, functional categories related to defense responses were over-represented (Fig. 8A). Among these were genes known to be transcriptionally induced by insect feeding (the β-glucosidase BGL1, At1g52400; and the lipoxygenase LOX2, At3g45140; Stotz et al., 2000) or wounding/jasmonate (e.g. jasmonate response JR1, At3g16470; Leon et al., 1998). Finally, genes involved in secondary metabolism are over-represented in the group of up-regulated genes (Fig. 8A). It is noteworthy that genes encoding enzymes involved in flavonoid and anthocyanin biosynthesis and transport are expressed to higher levels in the cyp98A3 insertion mutant; These include chalcone isomerase (CHI, At3g55120), dihydroflavonol 4-reductase (DFR, At5g42800), flavanone 3-hydroxylase (F3H, At3g51240), and gluthation transferase (TT19, At5g17220; Weisshaar and Jenkins, 1998; Kitamura et al., 2004
Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth
Visit the PubMed ArticleIn contrast, genes expressed to higher levels in cyp98A3 insertion mutants compared to wild type were over-represented in functional categories covering carbohydrate metabolism and include genes encoding enzymes in glycolysis (e.g. the glyceraldehyde 3-P dehydrogenase, At3g04120), the tricarboxylic acid cycle (e.g. the phosphoenolpyruvate carboxylase, At3g14940), and other anaerobic metabolisms (alcohol dehydrogenase, At1g77120). Also, functional categories related to defense responses were over-represented (Fig. 8A). Among these were genes known to be transcriptionally induced by insect feeding (the β-glucosidase BGL1, At1g52400; and the lipoxygenase LOX2, At3g45140; Stotz et al., 2000) or wounding/jasmonate (e.g. jasmonate response JR1, At3g16470; Leon et al., 1998). Finally, genes involved in secondary metabolism are over-represented in the group of up-regulated genes (Fig. 8A). It is noteworthy that genes encoding enzymes involved in flavonoid and anthocyanin biosynthesis and transport are expressed to higher levels in the cyp98A3 insertion mutant; These include chalcone isomerase (CHI, At3g55120), dihydroflavonol 4-reductase (DFR, At5g42800), flavanone 3-hydroxylase (F3H, At3g51240), and gluthation transferase (TT19, At5g17220; Weisshaar and Jenkins, 1998; Kitamura et al., 2004
Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth
Visit the PubMed ArticleSix of the 28 expansin family members represented on the array used are differentially expressed, significantly more than expected by chance. However, some expansin isoforms (EXP1, At1g69530; EXP10, At1g26770; and EXPR, At4g17030) are expressed to higher levels in cyp98A3 insertion mutants, whereas others (EXP8, At2g40610; EXP3, At2g37640; and EXP11, At1g20190) are down-regulated compared to levels found in wild-type plants
Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth
Visit the PubMed ArticleIndeed, several genes encoding proteins involved in the light-harvesting complex (e.g. chlorophyll a/b-binding proteins At2g34430, At3g08940, and At3g47470), in photosynthetic electron transport (e.g. the putative ferredoxin At1g10960 and proton gradient regulation 5, At2g05620; Munekage et al., 2002), and in the Calvin cycle (e.g. Rubisco At5g38410 and the putative Rib-5-P isomerase At3g04790) are strongly down-regulated in the mutant compared to the wild type (Supplemental Table I
Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth
Visit the PubMed ArticleSix of the 28 expansin family members represented on the array used are differentially expressed, significantly more than expected by chance. However, some expansin isoforms (EXP1, At1g69530; EXP10, At1g26770; and EXPR, At4g17030) are expressed to higher levels in cyp98A3 insertion mutants, whereas others (EXP8, At2g40610; EXP3, At2g37640; and EXP11, At1g20190) are down-regulated compared to levels found in wild-type plants
Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth
Visit the PubMed ArticleIn contrast, genes expressed to higher levels in cyp98A3 insertion mutants compared to wild type were over-represented in functional categories covering carbohydrate metabolism and include genes encoding enzymes in glycolysis (e.g. the glyceraldehyde 3-P dehydrogenase, At3g04120), the tricarboxylic acid cycle (e.g. the phosphoenolpyruvate carboxylase, At3g14940), and other anaerobic metabolisms (alcohol dehydrogenase, At1g77120). Also, functional categories related to defense responses were over-represented (Fig. 8A). Among these were genes known to be transcriptionally induced by insect feeding (the β-glucosidase BGL1, At1g52400; and the lipoxygenase LOX2, At3g45140; Stotz et al., 2000) or wounding/jasmonate (e.g. jasmonate response JR1, At3g16470; Leon et al., 1998). Finally, genes involved in secondary metabolism are over-represented in the group of up-regulated genes (Fig. 8A). It is noteworthy that genes encoding enzymes involved in flavonoid and anthocyanin biosynthesis and transport are expressed to higher levels in the cyp98A3 insertion mutant; These include chalcone isomerase (CHI, At3g55120), dihydroflavonol 4-reductase (DFR, At5g42800), flavanone 3-hydroxylase (F3H, At3g51240), and gluthation transferase (TT19, At5g17220; Weisshaar and Jenkins, 1998; Kitamura et al., 2004
Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth
Visit the PubMed ArticleThe total inactivation of the CYP98A3 gene in homozygous plants was confirmed by RNA-blot hybridization (Fig. 2C ) and quantitative reverse-transcription (RT)-PCR (see below
Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth
Visit the PubMed ArticleSix of the 28 expansin family members represented on the array used are differentially expressed, significantly more than expected by chance. However, some expansin isoforms (EXP1, At1g69530; EXP10, At1g26770; and EXPR, At4g17030) are expressed to higher levels in cyp98A3 insertion mutants, whereas others (EXP8, At2g40610; EXP3, At2g37640; and EXP11, At1g20190) are down-regulated compared to levels found in wild-type plants
Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth
Visit the PubMed ArticleGenes expressed to higher levels in the cyp98A3 mutant were clearly over-represented in the functional group related to jasmonate signaling (Fig. 8B). Indeed, many genes involved in the biosynthesis of jasmonate are significantly higher expressed in the mutant compared to wild type (Ws); These include LOX2 (see above), LOX1, allene oxide synthase (CYP74A, At5g42650), and allene oxide cyclase 4 (At1g13280). In addition, genes related to the abscisic acid cascade are over-represented in the group of genes expressed to higher levels in cyp98A3 insertion mutants (Fig. 8B). These include the gene responsive to dehydration 22 (RD22I, At5g25610), which is strongly induced by abscisic acid and drought (Yamaguchi-Shinozaki and Shinozaki, 1993), and the Arg decarboxylase 2 (ADC2, At4g34710) gene, which is strongly activated by abscisic acid, but also by methyl jasmonate and mechanical wounding, and is involved in polyamine biosynthesis (Perez-Amador et al., 2002). Also expressed to higher levels in cyp98A3 insertion mutants were two homeodomain Leu-zipper transcription factors (ATHB5, At5g65310; and ATHB7, At2g46680) involved in abscisic acid-mediated signaling pathways related to drought and osmotic stress (Söderman et al., 1996) and seed germination (Johannesson et al., 2003), respectively
Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth
Visit the PubMed ArticleSix of the 28 expansin family members represented on the array used are differentially expressed, significantly more than expected by chance. However, some expansin isoforms (EXP1, At1g69530; EXP10, At1g26770; and EXPR, At4g17030) are expressed to higher levels in cyp98A3 insertion mutants, whereas others (EXP8, At2g40610; EXP3, At2g37640; and EXP11, At1g20190) are down-regulated compared to levels found in wild-type plants
Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth
Visit the PubMed ArticleWhile transcript levels of genes encoding Phe ammonia lyase (PAL1; At2g37040) and HCT (At5g48930; Fig. 7A) were comparable in cyp98A3 mutants and wild type at all time points, an increase in C4H (CYP73A5; At2g30490) expression compared to wild type was observed at the 2-, 4-, and 8-h time points in cyp98A3 insertion mutants (Fig. 7A
Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth
Visit the PubMed ArticleIn contrast, genes expressed to higher levels in cyp98A3 insertion mutants compared to wild type were over-represented in functional categories covering carbohydrate metabolism and include genes encoding enzymes in glycolysis (e.g. the glyceraldehyde 3-P dehydrogenase, At3g04120), the tricarboxylic acid cycle (e.g. the phosphoenolpyruvate carboxylase, At3g14940), and other anaerobic metabolisms (alcohol dehydrogenase, At1g77120). Also, functional categories related to defense responses were over-represented (Fig. 8A). Among these were genes known to be transcriptionally induced by insect feeding (the β-glucosidase BGL1, At1g52400; and the lipoxygenase LOX2, At3g45140; Stotz et al., 2000) or wounding/jasmonate (e.g. jasmonate response JR1, At3g16470; Leon et al., 1998). Finally, genes involved in secondary metabolism are over-represented in the group of up-regulated genes (Fig. 8A). It is noteworthy that genes encoding enzymes involved in flavonoid and anthocyanin biosynthesis and transport are expressed to higher levels in the cyp98A3 insertion mutant; These include chalcone isomerase (CHI, At3g55120), dihydroflavonol 4-reductase (DFR, At5g42800), flavanone 3-hydroxylase (F3H, At3g51240), and gluthation transferase (TT19, At5g17220; Weisshaar and Jenkins, 1998; Kitamura et al., 2004
Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth
Visit the PubMed ArticleIndeed, several genes encoding proteins involved in the light-harvesting complex (e.g. chlorophyll a/b-binding proteins At2g34430, At3g08940, and At3g47470), in photosynthetic electron transport (e.g. the putative ferredoxin At1g10960 and proton gradient regulation 5, At2g05620; Munekage et al., 2002), and in the Calvin cycle (e.g. Rubisco At5g38410 and the putative Rib-5-P isomerase At3g04790) are strongly down-regulated in the mutant compared to the wild type (Supplemental Table I
Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth
Visit the PubMed ArticleWhile transcript levels of genes encoding Phe ammonia lyase (PAL1; At2g37040) and HCT (At5g48930; Fig. 7A) were comparable in cyp98A3 mutants and wild type at all time points, an increase in C4H (CYP73A5; At2g30490) expression compared to wild type was observed at the 2-, 4-, and 8-h time points in cyp98A3 insertion mutants (Fig. 7A
Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth
Visit the PubMed ArticleIn contrast, genes expressed to higher levels in cyp98A3 insertion mutants compared to wild type were over-represented in functional categories covering carbohydrate metabolism and include genes encoding enzymes in glycolysis (e.g. the glyceraldehyde 3-P dehydrogenase, At3g04120), the tricarboxylic acid cycle (e.g. the phosphoenolpyruvate carboxylase, At3g14940), and other anaerobic metabolisms (alcohol dehydrogenase, At1g77120). Also, functional categories related to defense responses were over-represented (Fig. 8A). Among these were genes known to be transcriptionally induced by insect feeding (the β-glucosidase BGL1, At1g52400; and the lipoxygenase LOX2, At3g45140; Stotz et al., 2000) or wounding/jasmonate (e.g. jasmonate response JR1, At3g16470; Leon et al., 1998). Finally, genes involved in secondary metabolism are over-represented in the group of up-regulated genes (Fig. 8A). It is noteworthy that genes encoding enzymes involved in flavonoid and anthocyanin biosynthesis and transport are expressed to higher levels in the cyp98A3 insertion mutant; These include chalcone isomerase (CHI, At3g55120), dihydroflavonol 4-reductase (DFR, At5g42800), flavanone 3-hydroxylase (F3H, At3g51240), and gluthation transferase (TT19, At5g17220; Weisshaar and Jenkins, 1998; Kitamura et al., 2004
Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth
Visit the PubMed ArticleIn contrast, genes expressed to higher levels in cyp98A3 insertion mutants compared to wild type were over-represented in functional categories covering carbohydrate metabolism and include genes encoding enzymes in glycolysis (e.g. the glyceraldehyde 3-P dehydrogenase, At3g04120), the tricarboxylic acid cycle (e.g. the phosphoenolpyruvate carboxylase, At3g14940), and other anaerobic metabolisms (alcohol dehydrogenase, At1g77120). Also, functional categories related to defense responses were over-represented (Fig. 8A). Among these were genes known to be transcriptionally induced by insect feeding (the β-glucosidase BGL1, At1g52400; and the lipoxygenase LOX2, At3g45140; Stotz et al., 2000) or wounding/jasmonate (e.g. jasmonate response JR1, At3g16470; Leon et al., 1998). Finally, genes involved in secondary metabolism are over-represented in the group of up-regulated genes (Fig. 8A). It is noteworthy that genes encoding enzymes involved in flavonoid and anthocyanin biosynthesis and transport are expressed to higher levels in the cyp98A3 insertion mutant; These include chalcone isomerase (CHI, At3g55120), dihydroflavonol 4-reductase (DFR, At5g42800), flavanone 3-hydroxylase (F3H, At3g51240), and gluthation transferase (TT19, At5g17220; Weisshaar and Jenkins, 1998; Kitamura et al., 2004
Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth
Visit the PubMed ArticleSix of the 28 expansin family members represented on the array used are differentially expressed, significantly more than expected by chance. However, some expansin isoforms (EXP1, At1g69530; EXP10, At1g26770; and EXPR, At4g17030) are expressed to higher levels in cyp98A3 insertion mutants, whereas others (EXP8, At2g40610; EXP3, At2g37640; and EXP11, At1g20190) are down-regulated compared to levels found in wild-type plants
Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth
Visit the PubMed ArticleIndeed, several genes encoding proteins involved in the light-harvesting complex (e.g. chlorophyll a/b-binding proteins At2g34430, At3g08940, and At3g47470), in photosynthetic electron transport (e.g. the putative ferredoxin At1g10960 and proton gradient regulation 5, At2g05620; Munekage et al., 2002), and in the Calvin cycle (e.g. Rubisco At5g38410 and the putative Rib-5-P isomerase At3g04790) are strongly down-regulated in the mutant compared to the wild type (Supplemental Table I
Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth
Visit the PubMed ArticleIn contrast, genes expressed to higher levels in cyp98A3 insertion mutants compared to wild type were over-represented in functional categories covering carbohydrate metabolism and include genes encoding enzymes in glycolysis (e.g. the glyceraldehyde 3-P dehydrogenase, At3g04120), the tricarboxylic acid cycle (e.g. the phosphoenolpyruvate carboxylase, At3g14940), and other anaerobic metabolisms (alcohol dehydrogenase, At1g77120). Also, functional categories related to defense responses were over-represented (Fig. 8A). Among these were genes known to be transcriptionally induced by insect feeding (the β-glucosidase BGL1, At1g52400; and the lipoxygenase LOX2, At3g45140; Stotz et al., 2000) or wounding/jasmonate (e.g. jasmonate response JR1, At3g16470; Leon et al., 1998). Finally, genes involved in secondary metabolism are over-represented in the group of up-regulated genes (Fig. 8A). It is noteworthy that genes encoding enzymes involved in flavonoid and anthocyanin biosynthesis and transport are expressed to higher levels in the cyp98A3 insertion mutant; These include chalcone isomerase (CHI, At3g55120), dihydroflavonol 4-reductase (DFR, At5g42800), flavanone 3-hydroxylase (F3H, At3g51240), and gluthation transferase (TT19, At5g17220; Weisshaar and Jenkins, 1998; Kitamura et al., 2004
Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth
Visit the PubMed ArticleGenes expressed to higher levels in the cyp98A3 mutant were clearly over-represented in the functional group related to jasmonate signaling (Fig. 8B). Indeed, many genes involved in the biosynthesis of jasmonate are significantly higher expressed in the mutant compared to wild type (Ws); These include LOX2 (see above), LOX1, allene oxide synthase (CYP74A, At5g42650), and allene oxide cyclase 4 (At1g13280). In addition, genes related to the abscisic acid cascade are over-represented in the group of genes expressed to higher levels in cyp98A3 insertion mutants (Fig. 8B). These include the gene responsive to dehydration 22 (RD22I, At5g25610), which is strongly induced by abscisic acid and drought (Yamaguchi-Shinozaki and Shinozaki, 1993), and the Arg decarboxylase 2 (ADC2, At4g34710) gene, which is strongly activated by abscisic acid, but also by methyl jasmonate and mechanical wounding, and is involved in polyamine biosynthesis (Perez-Amador et al., 2002). Also expressed to higher levels in cyp98A3 insertion mutants were two homeodomain Leu-zipper transcription factors (ATHB5, At5g65310; and ATHB7, At2g46680) involved in abscisic acid-mediated signaling pathways related to drought and osmotic stress (Söderman et al., 1996) and seed germination (Johannesson et al., 2003), respectively
Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth
Visit the PubMed ArticleGenes expressed to higher levels in the cyp98A3 mutant were clearly over-represented in the functional group related to jasmonate signaling (Fig. 8B). Indeed, many genes involved in the biosynthesis of jasmonate are significantly higher expressed in the mutant compared to wild type (Ws); These include LOX2 (see above), LOX1, allene oxide synthase (CYP74A, At5g42650), and allene oxide cyclase 4 (At1g13280). In addition, genes related to the abscisic acid cascade are over-represented in the group of genes expressed to higher levels in cyp98A3 insertion mutants (Fig. 8B). These include the gene responsive to dehydration 22 (RD22I, At5g25610), which is strongly induced by abscisic acid and drought (Yamaguchi-Shinozaki and Shinozaki, 1993), and the Arg decarboxylase 2 (ADC2, At4g34710) gene, which is strongly activated by abscisic acid, but also by methyl jasmonate and mechanical wounding, and is involved in polyamine biosynthesis (Perez-Amador et al., 2002). Also expressed to higher levels in cyp98A3 insertion mutants were two homeodomain Leu-zipper transcription factors (ATHB5, At5g65310; and ATHB7, At2g46680) involved in abscisic acid-mediated signaling pathways related to drought and osmotic stress (Söderman et al., 1996) and seed germination (Johannesson et al., 2003), respectively
Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth
Visit the PubMed ArticleIn contrast, genes expressed to higher levels in cyp98A3 insertion mutants compared to wild type were over-represented in functional categories covering carbohydrate metabolism and include genes encoding enzymes in glycolysis (e.g. the glyceraldehyde 3-P dehydrogenase, At3g04120), the tricarboxylic acid cycle (e.g. the phosphoenolpyruvate carboxylase, At3g14940), and other anaerobic metabolisms (alcohol dehydrogenase, At1g77120). Also, functional categories related to defense responses were over-represented (Fig. 8A). Among these were genes known to be transcriptionally induced by insect feeding (the β-glucosidase BGL1, At1g52400; and the lipoxygenase LOX2, At3g45140; Stotz et al., 2000) or wounding/jasmonate (e.g. jasmonate response JR1, At3g16470; Leon et al., 1998). Finally, genes involved in secondary metabolism are over-represented in the group of up-regulated genes (Fig. 8A). It is noteworthy that genes encoding enzymes involved in flavonoid and anthocyanin biosynthesis and transport are expressed to higher levels in the cyp98A3 insertion mutant; These include chalcone isomerase (CHI, At3g55120), dihydroflavonol 4-reductase (DFR, At5g42800), flavanone 3-hydroxylase (F3H, At3g51240), and gluthation transferase (TT19, At5g17220; Weisshaar and Jenkins, 1998; Kitamura et al., 2004
Abdulrazzak N, Pollet B, Ehlting J, Larsen K, Asnaghi C, Ronseau S, Proux C, Erhardt M, Seltzer V, Renou JP, Ullmann P, Pauly M, Lapierre C, Werck-Reichhart D - A coumaroyl-ester-3-hydroxylase insertion mutant reveals the existence of nonredundant meta-hydroxylation pathways and essential roles for phenolic precursors in cell expansion and plant growth
Visit the PubMed ArticleMIF1 expression was undetectable in the rosette leaf blade
Hu W, Ma H - Characterization of a novel putative zinc finger gene MIF1: involvement in multiple hormonal regulation of Arabidopsis development
Visit the PubMed Articlein 35S::MIF1 seedlings ... At1g62500, a member of the lipid transfer protein family that is similar to an auxin down-regulated gene in soybean (Glycine max), was found to be up-regulated
Hu W, Ma H - Characterization of a novel putative zinc finger gene MIF1: involvement in multiple hormonal regulation of Arabidopsis development
Visit the PubMed ArticleThe expression of ... the gibberellin-responsive GASA gene family ... At1g22690 (GAST2 homolog ... was significantly down-regulated ... in 35S::MIF1 seedlings
Hu W, Ma H - Characterization of a novel putative zinc finger gene MIF1: involvement in multiple hormonal regulation of Arabidopsis development
Visit the PubMed ArticleMIF1 was expressed ... very weakly in the petiole
Hu W, Ma H - Characterization of a novel putative zinc finger gene MIF1: involvement in multiple hormonal regulation of Arabidopsis development
Visit the PubMed ArticleThe expression of ... the gibberellin-responsive GASA gene family, At1g74670 (GAST1-like protein) ... was significantly down-regulated ... in 35S::MIF1 seedlings
Hu W, Ma H - Characterization of a novel putative zinc finger gene MIF1: involvement in multiple hormonal regulation of Arabidopsis development
Visit the PubMed Articlein 35S::MIF1 seedlings ... EXP2 ... up-regulated
Hu W, Ma H - Characterization of a novel putative zinc finger gene MIF1: involvement in multiple hormonal regulation of Arabidopsis development
Visit the PubMed ArticleReverse transcriptase–polymerase chain reaction (RT-PCR) analysis yielded results ... MIF1 expression was also detectable ... in the leaf, including the petiole
Hu W, Ma H - Characterization of a novel putative zinc finger gene MIF1: involvement in multiple hormonal regulation of Arabidopsis development
Visit the PubMed Articleincreased transcript levels of ... RGL3 ... in 35S::MIF1 seedlings
Hu W, Ma H - Characterization of a novel putative zinc finger gene MIF1: involvement in multiple hormonal regulation of Arabidopsis development
Visit the PubMed Articlein 35S::MIF1 seedlings ... XTH11 ... up-regulated
Hu W, Ma H - Characterization of a novel putative zinc finger gene MIF1: involvement in multiple hormonal regulation of Arabidopsis development
Visit the PubMed ArticleMIF2 was ... undetectable in the ... leaf
Hu W, Ma H - Characterization of a novel putative zinc finger gene MIF1: involvement in multiple hormonal regulation of Arabidopsis development
Visit the PubMed ArticleABI4 ... up-regulated in the dark-grown 35S::MIF1 seedlings
Hu W, Ma H - Characterization of a novel putative zinc finger gene MIF1: involvement in multiple hormonal regulation of Arabidopsis development
Visit the PubMed ArticleABI3 ... up-regulated in the dark-grown 35S::MIF1 seedlings
Hu W, Ma H - Characterization of a novel putative zinc finger gene MIF1: involvement in multiple hormonal regulation of Arabidopsis development
Visit the PubMed Articleincreased transcript levels of RGL2 ... in 35S::MIF1 seedlings
Hu W, Ma H - Characterization of a novel putative zinc finger gene MIF1: involvement in multiple hormonal regulation of Arabidopsis development
Visit the PubMed Articledecreased expression of RGL1 ... in 35S::MIF1 seedlings
Hu W, Ma H - Characterization of a novel putative zinc finger gene MIF1: involvement in multiple hormonal regulation of Arabidopsis development
Visit the PubMed ArticleThe expression of ... the gibberellin-responsive GASA gene family ... At2g14900, was significantly down-regulated ... in 35S::MIF1 seedlings
Hu W, Ma H - Characterization of a novel putative zinc finger gene MIF1: involvement in multiple hormonal regulation of Arabidopsis development
Visit the PubMed ArticleTwo GA 20-oxidases were significantly up-regulated in the dark-grown 35S::MIF1 seedlings (Table 2), consistent with the notion that GA biosynthesis is usually subject to negative feedback regulation (Olszewski et al., 2002). No other genes involved in GA metabolism, including GA 3-oxidases and GA 2-oxidases, showed significantly altered expression
Hu W, Ma H - Characterization of a novel putative zinc finger gene MIF1: involvement in multiple hormonal regulation of Arabidopsis development
Visit the PubMed Articlebdg cells accumulate higher levels of CER1 (∼184%) ... transcripts compared with wild-type plants
Kurdyukov S, Faust A, Nawrath C, Bär S, Voisin D, Efremova N, Franke R, Schreiber L, Saedler H, Métraux JP, Yephremov A - The epidermis-specific extracellular BODYGUARD controls cuticle development and morphogenesis in Arabidopsis
Visit the PubMed ArticleWith this method, the Alexa Fluor 488–specific fluorescent signals were detected in the epidermis of transgenic bdg BDG–Strep-tag plants (Figures 10B to 10G) but not in the bdg mutant (Figure 10A) or wild-type plants (data not shown). Although present throughout the epidermis of incipient and developing leaf primordia, BDG accumulated to higher levels on the adaxial side of organs (Figures 10B and 10C). In contrast with mRNA in situ hybridization (Figures 9F and 9G), a stronger signal intensity was not detected in the lateral regions of leaf primordia
Kurdyukov S, Faust A, Nawrath C, Bär S, Voisin D, Efremova N, Franke R, Schreiber L, Saedler H, Métraux JP, Yephremov A - The epidermis-specific extracellular BODYGUARD controls cuticle development and morphogenesis in Arabidopsis
Visit the PubMed ArticleA polarly localized fluorescence pattern was observed in epidermal cells, suggesting that BDG contains a motif required for polar protein sorting (Figures 10D to 10G). In the epidermis, the epitope-specific signal was localized in the outermost cell wall layer
Kurdyukov S, Faust A, Nawrath C, Bär S, Voisin D, Efremova N, Franke R, Schreiber L, Saedler H, Métraux JP, Yephremov A - The epidermis-specific extracellular BODYGUARD controls cuticle development and morphogenesis in Arabidopsis
Visit the PubMed ArticleTissue sections prepared from vegetative meristems and reproductive organs of wild-type Arabidopsis plants were hybridized with riboprobes directed against BDG mRNA ... in all organs analyzed (leaf primordia, immature floral buds, sepals, petals, and anthers), expression was found to be limited to epidermal cells
Kurdyukov S, Faust A, Nawrath C, Bär S, Voisin D, Efremova N, Franke R, Schreiber L, Saedler H, Métraux JP, Yephremov A - The epidermis-specific extracellular BODYGUARD controls cuticle development and morphogenesis in Arabidopsis
Visit the PubMed ArticleRNA gel blot hybridization revealed that whereas BDG is expressed in various organs, including roots, the strongest expression is detected in flowers and floral buds (see Supplemental Figure 2 online). Cauline leaves show higher levels of BDG transcripts than do rosette leaves
Kurdyukov S, Faust A, Nawrath C, Bär S, Voisin D, Efremova N, Franke R, Schreiber L, Saedler H, Métraux JP, Yephremov A - The epidermis-specific extracellular BODYGUARD controls cuticle development and morphogenesis in Arabidopsis
Visit the PubMed ArticleIn all organs of the BDG-GFP plants, GFP fluorescence was detected exclusively in protodermal and epidermal cells
Kurdyukov S, Faust A, Nawrath C, Bär S, Voisin D, Efremova N, Franke R, Schreiber L, Saedler H, Métraux JP, Yephremov A - The epidermis-specific extracellular BODYGUARD controls cuticle development and morphogenesis in Arabidopsis
Visit the PubMed Articlebdg cells accumulate higher levels of ... SHN1/WIN1 (∼158%) transcripts compared with wild-type plants
Kurdyukov S, Faust A, Nawrath C, Bär S, Voisin D, Efremova N, Franke R, Schreiber L, Saedler H, Métraux JP, Yephremov A - The epidermis-specific extracellular BODYGUARD controls cuticle development and morphogenesis in Arabidopsis
Visit the PubMed ArticleLeaf primordia exhibited higher levels of BDG expression in lateral regions
Kurdyukov S, Faust A, Nawrath C, Bär S, Voisin D, Efremova N, Franke R, Schreiber L, Saedler H, Métraux JP, Yephremov A - The epidermis-specific extracellular BODYGUARD controls cuticle development and morphogenesis in Arabidopsis
Visit the PubMed ArticleRNA in situ hybridization on seedlings and inflorescences reveals BB expression mainly in proliferating tissues, i.e., shoot and floral meristems and young organs
Disch S, Anastasiou E, Sharma VK, Laux T, Fletcher JC, Lenhard M - The E3 ubiquitin ligase BIG BROTHER controls arabidopsis organ size in a dosage-dependent manner
Visit the PubMed ArticleBy RT-PCR, BB mRNA can be detected in all plant organs tested
Disch S, Anastasiou E, Sharma VK, Laux T, Fletcher JC, Lenhard M - The E3 ubiquitin ligase BIG BROTHER controls arabidopsis organ size in a dosage-dependent manner
Visit the PubMed ArticleThe BB promoter is also active ... in developing embryos
Disch S, Anastasiou E, Sharma VK, Laux T, Fletcher JC, Lenhard M - The E3 ubiquitin ligase BIG BROTHER controls arabidopsis organ size in a dosage-dependent manner
Visit the PubMed ArticleBB expression ... in the vasculature
Disch S, Anastasiou E, Sharma VK, Laux T, Fletcher JC, Lenhard M - The E3 ubiquitin ligase BIG BROTHER controls arabidopsis organ size in a dosage-dependent manner
Visit the PubMed ArticleTo investigate whether ANT is nuclear localized, ANT was transiently expressed as a GFP fusion protein in leek epidermal cells. While GFP is present in both the cytoplasm and nucleus (Fig. 6a), the GFP–ANT protein is present exclusively in the nucleus (Fig. 6b
Krizek BA, Sulli C - Mapping sequences required for nuclear localization and the transcriptional activation function of the Arabidopsis protein AINTEGUMENTA
Visit the PubMed ArticleStrong expression of ... TRN2 was detected in ... leaf primordia
Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana
Visit the PubMed Articlein trn leaves ... little PDR5:GUS activity was detected at or around the vascular system of 8-d-old leaves 1 and 2
Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana
Visit the PubMed Articlein trn leaves ... little PDR5:GUS activity was detected at or around the vascular system of 8-d-old leaves 1 and 2
Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana
Visit the PubMed ArticlePCYCB1-1:GUS activity ... trn1-1 ... ectopic activity was noted in the vascular system
Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana
Visit the PubMed ArticleTRN1 ... Expression was also detected in the lamina of the cotyledons
Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana
Visit the PubMed ArticleStrong expression of TRN1 ... was detected in the SAM
Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana
Visit the PubMed ArticleStrong expression of TRN1 ... was detected in the ... leaf primordia
Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana
Visit the PubMed Articlein trn leaves ... ectopic GUS activity ... in the ... mesophyll layers
Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana
Visit the PubMed ArticleTRN1 ... expressed in ... leaves
Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana
Visit the PubMed Articletrn leaves were treated with α-naphthaleneacetic acid ... expression of PDR5:GUS ... more intense ... was observed in trn
Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana
Visit the PubMed ArticleStrong expression of ... TRN2 was detected in the SAM
Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana
Visit the PubMed Articletrn leaves were treated with α-naphthaleneacetic acid ... expression of PDR5:GUS ... more intense ... was observed in trn
Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana
Visit the PubMed ArticleTRN1 ... expressed in ... cell suspensions
Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana
Visit the PubMed ArticleTRN2 ... Expression was also detected in the lamina of the cotyledons
Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana
Visit the PubMed ArticleTRN1 ... expressed in seedlings
Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana
Visit the PubMed Articletopology prediction programs ... predict TRN1 to be cytosolic
Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana
Visit the PubMed Articlein trn leaves ... PDR5:GUS ... ectopic expression was observed along the margins
Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana
Visit the PubMed Articlein trn leaves ... PDR5:GUS ... ectopic expression was observed along the margins
Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana
Visit the PubMed Articlein trn leaves ... ectopic GUS activity ... in the ... mesophyll layers
Cnops G, Neyt P, Raes J, Petrarulo M, Nelissen H, Malenica N, Luschnig C, Tietz O, Ditengou F, Palme K, Azmi A, Prinsen E, Van Lijsebettens M - The TORNADO1 and TORNADO2 genes function in several patterning processes during early leaf development in Arabidopsis thaliana
Visit the PubMed ArticleRbcS3 ... abundant in both young-rosettes, 2.5 weeks of age, and mature rosettes, 4 weeks of age, but decline in abundance between 4 and 5 weeks of age
Duguay J, Jamal S, Liu Z, Wang TW, Thompson JE - Leaf-specific suppression of deoxyhypusine synthase in Arabidopsis thaliana enhances growth without negative pleiotropic effects
Visit the PubMed ArticleRbcS1 ... abundant in both young-rosettes, 2.5 weeks of age, and mature rosettes, 4 weeks of age, but decline in abundance between 4 and 5 weeks of age
Duguay J, Jamal S, Liu Z, Wang TW, Thompson JE - Leaf-specific suppression of deoxyhypusine synthase in Arabidopsis thaliana enhances growth without negative pleiotropic effects
Visit the PubMed ArticleThe temporal and spatial expression of DHS in Arabidopsis was examined by measuring GUS activity in transgenic plants expressing the transgene ProAtDHS:GUS. Quantitative fluorescence measurements of GUS in the developing 2nd rosette leaf pair revealed a small peak of DHS promoter activity at 3.5 weeks of age coincident with the onset of bolting and the initiation of nutrient translocation from rosette leaves, and a much more pronounced peak of activity at weeks 5 and 6, the period during which there is extensive cell death (Fig. 1). This bimodal pattern of DHS promoter activity temporally matches a similar pattern of DHS protein expression in senescing Arabidopsis rosettes observed previously
Duguay J, Jamal S, Liu Z, Wang TW, Thompson JE - Leaf-specific suppression of deoxyhypusine synthase in Arabidopsis thaliana enhances growth without negative pleiotropic effects
Visit the PubMed ArticleRbcS2 ... abundant in both young-rosettes, 2.5 weeks of age, and mature rosettes, 4 weeks of age, but decline in abundance between 4 and 5 weeks of age
Duguay J, Jamal S, Liu Z, Wang TW, Thompson JE - Leaf-specific suppression of deoxyhypusine synthase in Arabidopsis thaliana enhances growth without negative pleiotropic effects
Visit the PubMed ArticleHistochemical analysis of transgenic plants expressing ProAteIF5A1GUS indicated that AteIF5A1 is also expressed in the 2nd rosette leaf pair of 5-week-old plants
Duguay J, Jamal S, Liu Z, Wang TW, Thompson JE - Leaf-specific suppression of deoxyhypusine synthase in Arabidopsis thaliana enhances growth without negative pleiotropic effects
Visit the PubMed ArticleWe detected ICU4 transcripts in roots, vegetative leaves, shoots, flower buds, and open flowers of Col-0 by semiquantitative reverse transcription (RT)-PCR
Ochando I, Jover-Gil S, Ripoll JJ, Candela H, Vera A, Ponce MR, Martínez-Laborda A, Micol JL - Mutations in the microRNA complementarity site of the INCURVATA4 gene perturb meristem function and adaxialize lateral organs in arabidopsis
Visit the PubMed ArticleThe ICU4 gene has previously been shown to be expressed in the vasculature, as seen in plants carrying a pATHB-15::β-glucuronidase (GUS) transgene (Ohashi-Ito and Fukuda, 2003)
Ochando I, Jover-Gil S, Ripoll JJ, Candela H, Vera A, Ponce MR, Martínez-Laborda A, Micol JL - Mutations in the microRNA complementarity site of the INCURVATA4 gene perturb meristem function and adaxialize lateral organs in arabidopsis
Visit the PubMed ArticleTo test whether the gain-of-function icu4-1 mutation altered the leaf venation pattern, we studied the expression of the pATHB-8-GUS reporter transgene, which is restricted to provascular cells and has previously been used to characterize the development of the vascular system in leaves and stems (Baima et al., 1995; Kang and Dengler, 2002; Kang et al., 2003). The pattern of GUS staining revealed no differences between the wild-type and icu4-1/icu4-1 mutant plants
Ochando I, Jover-Gil S, Ripoll JJ, Candela H, Vera A, Ponce MR, Martínez-Laborda A, Micol JL - Mutations in the microRNA complementarity site of the INCURVATA4 gene perturb meristem function and adaxialize lateral organs in arabidopsis
Visit the PubMed ArticleKNAT2 was found to be misexpressed, with a 3-fold up-regulation in ... icu4-1/icu4-1 leaves
Ochando I, Jover-Gil S, Ripoll JJ, Candela H, Vera A, Ponce MR, Martínez-Laborda A, Micol JL - Mutations in the microRNA complementarity site of the INCURVATA4 gene perturb meristem function and adaxialize lateral organs in arabidopsis
Visit the PubMed ArticleICU4 ... transcript levels were 8-fold higher in icu4-1/icu4-1 leaves than in En-2 leaves, as is to be expected if icu4-1 escapes cleavage by the miRNA machinery
Ochando I, Jover-Gil S, Ripoll JJ, Candela H, Vera A, Ponce MR, Martínez-Laborda A, Micol JL - Mutations in the microRNA complementarity site of the INCURVATA4 gene perturb meristem function and adaxialize lateral organs in arabidopsis
Visit the PubMed ArticleTranscript levels of ICU4 were lower in the leaves than in the aerial tissues of En-2
Ochando I, Jover-Gil S, Ripoll JJ, Candela H, Vera A, Ponce MR, Martínez-Laborda A, Micol JL - Mutations in the microRNA complementarity site of the INCURVATA4 gene perturb meristem function and adaxialize lateral organs in arabidopsis
Visit the PubMed ArticleFIL was not upregulated in as1
Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway
Visit the PubMed ArticleETT ... upregulated in ... rdr6
Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway
Visit the PubMed ArticleARF4 ... upregulated in ... rdr6 ... and further increased in ... double mutants with as1
Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway
Visit the PubMed ArticlePHAVOLUTA ... Expression is re- stricted to the adaxial domain
Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway
Visit the PubMed ArticleARF4 ... upregulated in ... sgs3
Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway
Visit the PubMed ArticleARF4 ... upregulated significantly in ago7 as pre- viously described
Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway
Visit the PubMed ArticleETT ... upregulated in ago7
Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway
Visit the PubMed ArticleETT ... upregulated significantly in ago7 ... and were slightly further increased in as1 ago7 double mutants
Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway
Visit the PubMed ArticleSTM was also slightly upregulated in as1 whole seedlings, in contrast with leaves [35], but no additional upregulation was ob- served in the as1 ago7 double mutants, and the level of upregulation was too low to be detected by microarray analysis (see below
Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway
Visit the PubMed Articlein ... rdr6 ... double mutants with as1 ... FIL was upregulated
Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway
Visit the PubMed ArticleETT ... upregulated in ago7 ... and further increased in ... double mutants with as1
Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway
Visit the PubMed ArticleETT ... upregulated in ... sgs3 ... and further increased in ... double mutants with as1
Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway
Visit the PubMed ArticleFIL was not upregulated ... in ago7
Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway
Visit the PubMed ArticleWe first assayed BP accumulation in as1 and as1 ago7 (Figure S2J), but we could not detect further overexpres- sion in the double mutants, although we could not rule out changes in transcript localization
Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway
Visit the PubMed Articlein ago7 ... double mutants with as1 ... FIL was upregulated
Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway
Visit the PubMed ArticleREVOLUTA ... Expression is re- stricted to the adaxial domain
Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway
Visit the PubMed ArticleARF4 ... upregulated significantly in ago7 ... and were slightly further increased in as1 ago7 double mutants
Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway
Visit the PubMed Articleoverexpression of FIL in the as1 ago7 double mutant was reduced in the as1 ago7 ett triple mutant
Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway
Visit the PubMed Articlein ... sgs3 ... double mutants with as1 ... FIL was upregulated
Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway
Visit the PubMed Articlein as1 ago7 ... FIL ... was strongly upregu- lated in the double mutant
Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway
Visit the PubMed ArticleFIL ... was strongly upregu- lated in ... as2 rdr6
Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway
Visit the PubMed ArticleWe found that a gene-trap reporter gene integrated into the promoter of TAS3 was expressed on the adaxial side of early leaf primordia
Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway
Visit the PubMed ArticleThe microRNA miR165 ... is expressed in the abaxial domain in older primordia
Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway
Visit the PubMed ArticleETT ... upregulated in ... sgs3
Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway
Visit the PubMed ArticleETT ... upregulated in ... rdr6 ... and further increased in ... double mutants with as1
Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway
Visit the PubMed ArticleBP was strongly upregulated in as1 mutants compared to wild-type plants
Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway
Visit the PubMed ArticleARF4 ... upregulated in ago7
Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway
Visit the PubMed ArticleBP was strongly upregulated in as1 mutants compared to wild-type plants, but no additional upregu- lation was observed in as1 ago7 double mutants
Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway
Visit the PubMed ArticleARF4 ... upregulated in ago7 ... and further increased in ... double mutants with as1
Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway
Visit the PubMed ArticleWe found that a gene-trap reporter gene integrated into the promoter of TAS3 was expressed ... in stipules
Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway
Visit the PubMed ArticleETT ... upregulated significantly in ago7 as pre- viously described
Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway
Visit the PubMed ArticlePHABULOSA ... Expression is re- stricted to the adaxial domain
Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway
Visit the PubMed ArticleARF4 ... upregulated in ... rdr6
Garcia D, Collier SA, Byrne ME, Martienssen RA - Specification of leaf polarity in Arabidopsis via the trans-acting siRNA pathway
Visit the PubMed ArticleUsing quantitative RT (qRT)-PCR, we studied the expression of the RpoTm ... in 4-d-old seedlings (showing only expanded cotyledons), 12-d-old seedlings (showing expanded cotyledons and four expanding leaves), 3-week-old rosettes (see Fig. 1, G and H), and roots ... found to be expressed in all the organs and stages analyzed in wild-type ... plants. In Col-0, RpoTm reached the highest level of expression in 12-d-old seedlings, where it was 1.9-, 1.5-, and 2.1-fold higher than in 4-d-old seedlings, rosettes, and roots, respectively
Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis
Visit the PubMed ArticleqRT-PCR amplifications were performed on RNA extracted from 4- and 12-d-old seedlings and 21-d-old rosettes of Col-0 and the sca3-2 mutant ... sca3-2 mutant ... rpoC1 ... down-regulated in ... all the developmental stages studied
Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis
Visit the PubMed ArticleIn the sca3-2 mutant, the LCHB2 ... 6.4 ... fold down-regulated by qRT-PCR, whereas microarray analyses showed 3.7 ... fold levels
Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis
Visit the PubMed ArticleArabidopsis in 4-d-old seedlings (showing only expanded cotyledons), 12-d-old seedlings (showing expanded cotyledons and four expanding leaves), 3-week-old rosettes (see Fig. 1, G and H), and roots ... in ... sca3-2/sca3-2 plants ... RpoTm ... reduction was found ... in 12-d-old seedlings
Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis
Visit the PubMed ArticleIn the sca3-2 mutant ... PSAK ... 2.8 ... fold down-regulated by qRT-PCR, whereas microarray analyses showed ... 2.1 ... fold levels
Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis
Visit the PubMed ArticleqRT-PCR amplifications were performed on RNA extracted from 4- and 12-d-old seedlings and 21-d-old rosettes of Col-0 and the sca3-2 mutant ... transcript levels were reduced in sca3-2/sca3-2 ... of rps18 only in 4-d-old seedlings
Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis
Visit the PubMed ArticleqRT-PCR amplifications were performed on RNA extracted from 4- and 12-d-old seedlings and 21-d-old rosettes of Col-0 and the sca3-2 mutant ... transcript levels of ... clpP were reduced in 4-d-old seedlings ... in sca3-2/sca3-2
Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis
Visit the PubMed ArticleArabidopsis in 4-d-old seedlings (showing only expanded cotyledons), 12-d-old seedlings (showing expanded cotyledons and four expanding leaves), 3-week-old rosettes (see Fig. 1, G and H), and roots ... in ... sca3-2/sca3-2 plants ... RpoTmp ... a reduction was found ... in 12-d-old seedlings
Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis
Visit the PubMed ArticleqRT-PCR amplifications were performed on RNA extracted from 4- and 12-d-old seedlings and 21-d-old rosettes of Col-0 and the sca3-2 mutant ... transcript levels of accD and ... were ... increased in 12-d-old seedlings ... in sca3-2/sca3-2
Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis
Visit the PubMed Articledown-regulated in sca3-2 ... CLB6
Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis
Visit the PubMed Articledown-regulated in sca3-2 ... CHLOROPLAST BIOGENESIS 4 ... CLB4; hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase
Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis
Visit the PubMed ArticleUsing quantitative RT (qRT)-PCR, we studied the expression ... SCA3 (RpoTp) ... 4-d-old seedlings (showing only expanded cotyledons), 12-d-old seedlings (showing expanded cotyledons and four expanding leaves), 3-week-old rosettes (see Fig. 1, G and H), and roots ... found to be expressed in all the organs and stages analyzed in wild-type ... SCA3 ... predominantly expressed in rosettes ... transcripts accumulated 3.3-, 2.5-, and 2.8-fold higher than in 4- and 12-d-old seedlings and roots, respectively
Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis
Visit the PubMed ArticleTo validate our microarray results, we used qRT-PCR to analyze the expression of five genes in 3-week-old sca3-2/sca3-2 and Col-0 plants. SEP3 was 7.0- and 2.7-fold up-regulated in sca3-2 compared with Col-0, as detected by qRT-PCR and microarray analysis, respectively
Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis
Visit the PubMed ArticleqRT-PCR amplifications were performed on RNA extracted from 4- and 12-d-old seedlings and 21-d-old rosettes of Col-0 and the sca3-2 mutant ... transcript levels of ... clpP were ... increased in 12-d-old seedlings ... in sca3-2/sca3-2
Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis
Visit the PubMed Articledown-regulated in sca3-2 ... the RNAP σ-subunit SigA ... SIG1
Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis
Visit the PubMed ArticleArabidopsis in 4-d-old seedlings (showing only expanded cotyledons), 12-d-old seedlings (showing expanded cotyledons and four expanding leaves), 3-week-old rosettes (see Fig. 1, G and H), and roots ... in ... sca3-2/sca3-2 plants ... RpoTm ... showed higher transcript levels than in Col-0 in 4-d-old seedlings and 21-d-old plants
Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis
Visit the PubMed ArticleqRT-PCR amplifications were performed on RNA extracted from 4- and 12-d-old seedlings and 21-d-old rosettes of Col-0 and the sca3-2 mutant ... transcript levels of accD ... were reduced in 4-d-old seedlings ... in sca3-2/sca3-2
Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis
Visit the PubMed ArticleqRT-PCR amplifications were performed on RNA extracted from 4- and 12-d-old seedlings and 21-d-old rosettes of Col-0 and the sca3-2 mutant ... sca3-2 mutant ... rpoB ... down-regulated in all the developmental stages studied
Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis
Visit the PubMed Articledown-regulated in sca3-2 ... CLA1 ... encoding the 1-deoxy-D-xylulose-5-P synthase
Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis
Visit the PubMed ArticleclpP transcript levels were reduced in sca3-2/sca3-2 rosettes
Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis
Visit the PubMed ArticleSCA3 was down-regulated in the mutant in all developmental stages studied, especially in 4-d-old seedlings
Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis
Visit the PubMed Articledown-regulated in sca3-2 ... NCED4
Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis
Visit the PubMed Articledown-regulated in sca3-2 ... ABSCISIC ACID1 ... ABA1; zeaxanthin epoxidase
Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis
Visit the PubMed ArticleIn the sca3-2 mutant, the ... SIG1 ... 2.1 ... fold down-regulated by qRT-PCR, whereas microarray analyses showed ... 1.7 ... fold levels
Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis
Visit the PubMed ArticleUsing quantitative RT (qRT)-PCR, we studied the expression of ... RpoTmp ... in 4-d-old seedlings (showing only expanded cotyledons), 12-d-old seedlings (showing expanded cotyledons and four expanding leaves), 3-week-old rosettes (see Fig. 1, G and H), and roots ... found to be expressed in all the organs and stages analyzed in wild-type ... RpoTmp ... predominantly expressed in rosettes
Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis
Visit the PubMed Articlewe performed a microarray analysis using RNA extracted from 3-week-old plants of the sca3-2 ... the most up-regulated gene found in the microarray was the MADS-box family member SEP3
Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis
Visit the PubMed ArticleArabidopsis in 4-d-old seedlings (showing only expanded cotyledons), 12-d-old seedlings (showing expanded cotyledons and four expanding leaves), 3-week-old rosettes (see Fig. 1, G and H), and roots ... in ... sca3-2/sca3-2 plants ... RpoTmp showed higher transcript levels than in Col-0 in 4-d-old seedlings and 21-d-old plants
Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis
Visit the PubMed ArticleSCA3 gene ... is targeted exclusively to plastids (Hedtke et al., 1997, 1999)
Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis
Visit the PubMed ArticleIn the sca3-2 mutant ... ABA1 ... 1.6-fold down-regulated by qRT-PCR, whereas microarray analyses showed ... 1.7-fold levels
Hricová A, Quesada V, Micol JL - The SCABRA3 nuclear gene encodes the plastid RpoTp RNA polymerase, which is required for chloroplast biogenesis and mesophyll cell proliferation in Arabidopsis
Visit the PubMed Articleleaves of the sgs3-11 as2-101 ... contained ... reduced levels of REV transcripts
Xu L, Yang L, Pi L, Liu Q, Ling Q, Wang H, Poethig RS, Huang H - Genetic interaction between the AS1-AS2 and RDR6-SGS3-AGO7 pathways for leaf morphogenesis
Visit the PubMed ArticlemiR165/166 ... transcript levels were ... elevated in the rdr6-3 as2-101 leaves
Xu L, Yang L, Pi L, Liu Q, Ling Q, Wang H, Poethig RS, Huang H - Genetic interaction between the AS1-AS2 and RDR6-SGS3-AGO7 pathways for leaf morphogenesis
Visit the PubMed ArticleREV transcripts were detected in the earlier stage leaf primordia in ... zip as1-101
Xu L, Yang L, Pi L, Liu Q, Ling Q, Wang H, Poethig RS, Huang H - Genetic interaction between the AS1-AS2 and RDR6-SGS3-AGO7 pathways for leaf morphogenesis
Visit the PubMed Articleleaves of the ... zip as2-101 ... contained ... reduced levels of REV transcripts
Xu L, Yang L, Pi L, Liu Q, Ling Q, Wang H, Poethig RS, Huang H - Genetic interaction between the AS1-AS2 and RDR6-SGS3-AGO7 pathways for leaf morphogenesis
Visit the PubMed ArticleREV ... repressed ... in the rdr6-3 as2-101 leaves
Xu L, Yang L, Pi L, Liu Q, Ling Q, Wang H, Poethig RS, Huang H - Genetic interaction between the AS1-AS2 and RDR6-SGS3-AGO7 pathways for leaf morphogenesis
Visit the PubMed ArticlePHB ... repressed ... in the rdr6-3 as2-101 leaves
Xu L, Yang L, Pi L, Liu Q, Ling Q, Wang H, Poethig RS, Huang H - Genetic interaction between the AS1-AS2 and RDR6-SGS3-AGO7 pathways for leaf morphogenesis
Visit the PubMed Articleleaves of the ... zip as2-101 ... double mutant plants contained dramatically enhanced miR165/ 166 levels
Xu L, Yang L, Pi L, Liu Q, Ling Q, Wang H, Poethig RS, Huang H - Genetic interaction between the AS1-AS2 and RDR6-SGS3-AGO7 pathways for leaf morphogenesis
Visit the PubMed ArticleREV transcripts were detected in the earlier stage leaf primordia in ... sgs3-11 as2-101
Xu L, Yang L, Pi L, Liu Q, Ling Q, Wang H, Poethig RS, Huang H - Genetic interaction between the AS1-AS2 and RDR6-SGS3-AGO7 pathways for leaf morphogenesis
Visit the PubMed ArticleFIL transcript levels were ... elevated in the rdr6-3 as2-101 leaves
Xu L, Yang L, Pi L, Liu Q, Ling Q, Wang H, Poethig RS, Huang H - Genetic interaction between the AS1-AS2 and RDR6-SGS3-AGO7 pathways for leaf morphogenesis
Visit the PubMed Articleleaves of the sgs3-11 as2-101 ... double mutant plants contained dramatically enhanced miR165/ 166 levels
Xu L, Yang L, Pi L, Liu Q, Ling Q, Wang H, Poethig RS, Huang H - Genetic interaction between the AS1-AS2 and RDR6-SGS3-AGO7 pathways for leaf morphogenesis
Visit the PubMed Articlerdr6-3 as2-101 ... leaves ... contained ... reduced levels of REV transcripts
Xu L, Yang L, Pi L, Liu Q, Ling Q, Wang H, Poethig RS, Huang H - Genetic interaction between the AS1-AS2 and RDR6-SGS3-AGO7 pathways for leaf morphogenesis
Visit the PubMed ArticleREV transcripts were detected in the earlier stage leaf primordia in ... zip as2-101
Xu L, Yang L, Pi L, Liu Q, Ling Q, Wang H, Poethig RS, Huang H - Genetic interaction between the AS1-AS2 and RDR6-SGS3-AGO7 pathways for leaf morphogenesis
Visit the PubMed ArticleREV transcripts were detected in the earlier stage leaf primordia in sgs3-11 as1-101
Xu L, Yang L, Pi L, Liu Q, Ling Q, Wang H, Poethig RS, Huang H - Genetic interaction between the AS1-AS2 and RDR6-SGS3-AGO7 pathways for leaf morphogenesis
Visit the PubMed Articlethe FILAMENTOUS FLOWER (FIL) gene that is known to promote abaxial leaf identity (Bowman 2000, Eshed et al. 2001) was expressed more abundantly in the ago1-27 as2-101 leaves than in the ago1-27 and as2-101 single mutant leaves
Xu L, Yang L, Pi L, Liu Q, Ling Q, Wang H, Poethig RS, Huang H - Genetic interaction between the AS1-AS2 and RDR6-SGS3-AGO7 pathways for leaf morphogenesis
Visit the PubMed ArticleTo investigate OBP2 expression at the cell and tissue level, approximately 1 kb of the 5′ upstream regulatory region of OBP2 was fused to the E. coliβ-glucuronidase (GUS) reporter gene and transferred to the nuclear genome of Arabidopsis ... In leaves, GUS expression in the vasculature was strongest in phloem cells
Skirycz A, Reichelt M, Burow M, Birkemeyer C, Rolcik J, Kopka J, Zanor MI, Gershenzon J, Strnad M, Szopa J, Mueller-Roeber B, Witt I - DOF transcription factor AtDof1
Visit the PubMed ArticleTo reduce OBP2 transcript level, we transformed Arabidopsis plants with an RNAi–OBP2 construct ... CYP79 ... B3 ... significantly reduced in just one line
Skirycz A, Reichelt M, Burow M, Birkemeyer C, Rolcik J, Kopka J, Zanor MI, Gershenzon J, Strnad M, Szopa J, Mueller-Roeber B, Witt I - DOF transcription factor AtDof1
Visit the PubMed Articlemechanical wounding, which stimulates jasmonate biosynthesis, enhanced OBP2 transcript level
Skirycz A, Reichelt M, Burow M, Birkemeyer C, Rolcik J, Kopka J, Zanor MI, Gershenzon J, Strnad M, Szopa J, Mueller-Roeber B, Witt I - DOF transcription factor AtDof1
Visit the PubMed ArticleTo reduce OBP2 transcript level, we transformed Arabidopsis plants with an RNAi–OBP2 construct ... CYP79B2 ... significantly reduced in just one line
Skirycz A, Reichelt M, Burow M, Birkemeyer C, Rolcik J, Kopka J, Zanor MI, Gershenzon J, Strnad M, Szopa J, Mueller-Roeber B, Witt I - DOF transcription factor AtDof1
Visit the PubMed ArticleTargeted expression analysis on 35S:OBP2 plants using quantitative real-time PCR (Q-RTPCR) confirmed the increased transcript levels of ... ATR1 ... identified by transcript profiling
Skirycz A, Reichelt M, Burow M, Birkemeyer C, Rolcik J, Kopka J, Zanor MI, Gershenzon J, Strnad M, Szopa J, Mueller-Roeber B, Witt I - DOF transcription factor AtDof1
Visit the PubMed ArticleTargeted expression analysis on 35S:OBP2 plants using quantitative real-time PCR (Q-RTPCR ... showed increases for CYP83B1 ... which were not represented on the array
Skirycz A, Reichelt M, Burow M, Birkemeyer C, Rolcik J, Kopka J, Zanor MI, Gershenzon J, Strnad M, Szopa J, Mueller-Roeber B, Witt I - DOF transcription factor AtDof1
Visit the PubMed ArticleUsing semi-quantitative RT-PCR, OBP2 was previously found to be expressed in all organs of 6-week-old Arabidopsis plants, revealing the highest expression in roots and leaves, and a comparatively weak expression in stems and flowers
Skirycz A, Reichelt M, Burow M, Birkemeyer C, Rolcik J, Kopka J, Zanor MI, Gershenzon J, Strnad M, Szopa J, Mueller-Roeber B, Witt I - DOF transcription factor AtDof1
Visit the PubMed ArticleTo reduce OBP2 transcript level, we transformed Arabidopsis plants with an RNAi–OBP2 construct ... CYP83B1 was significantly reduced in two lines
Skirycz A, Reichelt M, Burow M, Birkemeyer C, Rolcik J, Kopka J, Zanor MI, Gershenzon J, Strnad M, Szopa J, Mueller-Roeber B, Witt I - DOF transcription factor AtDof1
Visit the PubMed ArticleTargeted expression analysis on 35S:OBP2 plants using quantitative real-time PCR (Q-RTPCR ... showed increases for ... MAM-1 ... which were not represented on the array
Skirycz A, Reichelt M, Burow M, Birkemeyer C, Rolcik J, Kopka J, Zanor MI, Gershenzon J, Strnad M, Szopa J, Mueller-Roeber B, Witt I - DOF transcription factor AtDof1
Visit the PubMed ArticleNorthern blot analysis indicated that the OBP2 transcript level increased within 4–6 h upon external MeJA application
Skirycz A, Reichelt M, Burow M, Birkemeyer C, Rolcik J, Kopka J, Zanor MI, Gershenzon J, Strnad M, Szopa J, Mueller-Roeber B, Witt I - DOF transcription factor AtDof1
Visit the PubMed ArticleTo reduce OBP2 transcript level, we transformed Arabidopsis plants with an RNAi–OBP2 construct ... MAM-1 ... not changed
Skirycz A, Reichelt M, Burow M, Birkemeyer C, Rolcik J, Kopka J, Zanor MI, Gershenzon J, Strnad M, Szopa J, Mueller-Roeber B, Witt I - DOF transcription factor AtDof1
Visit the PubMed ArticleTargeted expression analysis on 35S:OBP2 plants using quantitative real-time PCR (Q-RTPCR) confirmed the increased transcript levels of CYP79B2 ... identified by transcript profiling
Skirycz A, Reichelt M, Burow M, Birkemeyer C, Rolcik J, Kopka J, Zanor MI, Gershenzon J, Strnad M, Szopa J, Mueller-Roeber B, Witt I - DOF transcription factor AtDof1
Visit the PubMed ArticleTargeted expression analysis on 35S:OBP2 plants using quantitative real-time PCR (Q-RTPCR) confirmed the increased transcript levels of CYP79 ... B3 ... identified by transcript profiling
Skirycz A, Reichelt M, Burow M, Birkemeyer C, Rolcik J, Kopka J, Zanor MI, Gershenzon J, Strnad M, Szopa J, Mueller-Roeber B, Witt I - DOF transcription factor AtDof1
Visit the PubMed ArticleTo reduce OBP2 transcript level, we transformed Arabidopsis plants with an RNAi–OBP2 construct ... ATR1 ... not changed
Skirycz A, Reichelt M, Burow M, Birkemeyer C, Rolcik J, Kopka J, Zanor MI, Gershenzon J, Strnad M, Szopa J, Mueller-Roeber B, Witt I - DOF transcription factor AtDof1
Visit the PubMed ArticleARGOS transcripts were highly induced by auxin
Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth
Visit the PubMed Articlethe induction of ARL by epi-BL was ... still detectable in the BR-deficient mutant det2-1
Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth
Visit the PubMed ArticleARL expression was detected ... not in leaf primordia
Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth
Visit the PubMed ArticleARL expression was ... also observed in expanding leaves
Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth
Visit the PubMed Articlethe intracellular localization of ARL by transient expression of a 35S-ARL–GFP or a 35S-GFP–ARL fusion gene in onion epidermal cells using green fluorescent protein (GFP) alone as a control. The intracellular distribution of both fusion proteins was similar to that of GFP alone (Figure 1c), indicating no specific cellular localization of ARL to any cellular compartment
Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth
Visit the PubMed ArticleTCH4 gene is expressed in expanding cells ... and is responsive to some plant hormones and stimuli, including auxin, BR and touch
Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth
Visit the PubMed ArticleARL transcript were ... somewhat repressed by ... GA treatments
Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth
Visit the PubMed ArticleARL transcript were just slightly elevated by ... cytokinin
Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth
Visit the PubMed ArticleARL transcript were just slightly elevated by auxin
Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth
Visit the PubMed ArticleARL transcript were ... somewhat repressed by ABA
Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth
Visit the PubMed ArticleARL expression was detected throughout the cotyledons
Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth
Visit the PubMed Articlean apparent induction of ARL (2.2-fold) was found when seedlings were treated with epi-brassinolide (epi-BL)
Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth
Visit the PubMed ArticleARGOS transcripts were highly induced by ... cytokinin
Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth
Visit the PubMed Articletranscript levels of TCH4 were reduced in ARLi lines
Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth
Visit the PubMed ArticleARGOS transcripts ... remained almost unchanged under epi-BL treatment
Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth
Visit the PubMed ArticleARL expression was detected ... not in ... juvenile leaves
Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth
Visit the PubMed Articletranscript levels of TCH4 were ... elevated in ARL-OE lines
Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth
Visit the PubMed Articlethe induction of ARL by epi-BL was disrupted in bri1–119
Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth
Visit the PubMed ArticleARGOS observed only in juvenile leaves
Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth
Visit the PubMed ArticleARGOS observed ... in ... vascular tissues of cotyledons
Hu Y, Poh HM, Chua NH - The Arabidopsis ARGOS-LIKE gene regulates cell expansion during organ growth
Visit the PubMed ArticleDividing, but not mature, leaf cells contained high SGB1 levels
Wang HX, Weerasinghe RR, Perdue TD, Cakmakci NG, Taylor JP, Marzluff WF, Jones AM - A Golgi-localized hexose transporter is involved in heterotrimeric G protein-mediated early development in Arabidopsis
Visit the PubMed ArticleApplication of d-glucose greatly increased the steady-state level of the SGB1-GUS fusion protein and its distribution pattern
Wang HX, Weerasinghe RR, Perdue TD, Cakmakci NG, Taylor JP, Marzluff WF, Jones AM - A Golgi-localized hexose transporter is involved in heterotrimeric G protein-mediated early development in Arabidopsis
Visit the PubMed ArticleSGB1 RNA was detected in various tissues of wild-type plants by RT-PCR
Wang HX, Weerasinghe RR, Perdue TD, Cakmakci NG, Taylor JP, Marzluff WF, Jones AM - A Golgi-localized hexose transporter is involved in heterotrimeric G protein-mediated early development in Arabidopsis
Visit the PubMed ArticleNeither the absence ... of SGB1 had an effect on AGB1 gene expression
Wang HX, Weerasinghe RR, Perdue TD, Cakmakci NG, Taylor JP, Marzluff WF, Jones AM - A Golgi-localized hexose transporter is involved in heterotrimeric G protein-mediated early development in Arabidopsis
Visit the PubMed ArticleA specific set of newly formed cells of vascular traces in expanding leaves was shown to contain SGB1
Wang HX, Weerasinghe RR, Perdue TD, Cakmakci NG, Taylor JP, Marzluff WF, Jones AM - A Golgi-localized hexose transporter is involved in heterotrimeric G protein-mediated early development in Arabidopsis
Visit the PubMed ArticleSimilarly, sucrose increased the steady-state level of SGB1-GUS but with less efficacy. Sorbitol, inositol, and fructose did not alter the levels or distribution pattern of SGB1 over the no-sugar control
Wang HX, Weerasinghe RR, Perdue TD, Cakmakci NG, Taylor JP, Marzluff WF, Jones AM - A Golgi-localized hexose transporter is involved in heterotrimeric G protein-mediated early development in Arabidopsis
Visit the PubMed ArticleSGB1 protein was also high in ... embryos
Wang HX, Weerasinghe RR, Perdue TD, Cakmakci NG, Taylor JP, Marzluff WF, Jones AM - A Golgi-localized hexose transporter is involved in heterotrimeric G protein-mediated early development in Arabidopsis
Visit the PubMed ArticleSGB1-YFP driven by the native promoter in wild-type (Figure 7B) and agb1-2 null (Figure 7C) backgrounds showed that fluorescence was observed in root tissues where the SGB1 promoter is active (Figure 6, B and C). Moreover, the fluorescence imaging was observed to be in multiple punctate compartments within each cell, reminiscent of the size, distribution, and number of Golgi apparatus in plant cells (Boevink et al., 1998 blue right-pointing triangle). Moreover, the dynamics of SGB1-GFP movement (our unpublished data) also matched published Golgi dynamics ... To confirm that the observed fluorescence was the Golgi apparatus, BFA was used. BFA has been shown in plants to disrupt the integrity of Golgi stacks, resulting in the fusion of the endoplasmic reticulum (ER) and the Golgi stacks and the formation of so called “BFA compartments” (Ritzenthaler et al., 2002 blue right-pointing triangle). After treatment with brefeldin-A (Figure 7D, bottom), the GFP fluorescence was observed to change from punctate structures representing the Golgi to both a diffuse fluorescence representing the fused ER/Golgi compartments and larger punctate structures representing the newly formed BFA compartments (Ritzenthaler et al., 2002 blue right-pointing triangle). Z-stack reconstruction imaging of the young leaf epidermal cells of SGB1-GFP plants before (Figure 7E, untreated) and after BFA treatment (Figure 7D, +100 μM BFA) clearly demonstrate the effect of BFA on SGB1 localization. Higher magnification imaging of an individual BFA compartment (Figure 7, F and G) demonstrated that the larger BFA compartments were made up of fused smaller compartments, presumably individual Golgi stacks. Figure 7F is a three-dimensional reconstruction from a Z-stack acquisition of control and BFA-treated cells
Wang HX, Weerasinghe RR, Perdue TD, Cakmakci NG, Taylor JP, Marzluff WF, Jones AM - A Golgi-localized hexose transporter is involved in heterotrimeric G protein-mediated early development in Arabidopsis
Visit the PubMed Articlenor excessive amount of SGB1 had an effect on AGB1 gene expression
Wang HX, Weerasinghe RR, Perdue TD, Cakmakci NG, Taylor JP, Marzluff WF, Jones AM - A Golgi-localized hexose transporter is involved in heterotrimeric G protein-mediated early development in Arabidopsis
Visit the PubMed ArticleRE expression ... in the wild types ... roots, rosette leaves, stems, flower buds, and open flowers of Col-0, En-2 and Ler
González-Bayón R, Kinsman EA, Quesada V, Vera A, Robles P, Ponce MR, Pyke KA, Micol JL - Mutations in the RETICULATA gene dramatically alter internal architecture but have little effect on overall organ shape in Arabidopsis leaves
Visit the PubMed ArticleRE expression ... GUS activity was also detected in stipules
González-Bayón R, Kinsman EA, Quesada V, Vera A, Robles P, Ponce MR, Pyke KA, Micol JL - Mutations in the RETICULATA gene dramatically alter internal architecture but have little effect on overall organ shape in Arabidopsis leaves
Visit the PubMed ArticleRE expression ... GUS activity was also detected in ... cauline leaves
González-Bayón R, Kinsman EA, Quesada V, Vera A, Robles P, Ponce MR, Pyke KA, Micol JL - Mutations in the RETICULATA gene dramatically alter internal architecture but have little effect on overall organ shape in Arabidopsis leaves
Visit the PubMed ArticleRE expression ... GUS activity ... decreased as leaves aged
González-Bayón R, Kinsman EA, Quesada V, Vera A, Robles P, Ponce MR, Pyke KA, Micol JL - Mutations in the RETICULATA gene dramatically alter internal architecture but have little effect on overall organ shape in Arabidopsis leaves
Visit the PubMed ArticleRE expression ... GUS activity was high in the vasculature of developing leaf primordia
González-Bayón R, Kinsman EA, Quesada V, Vera A, Robles P, Ponce MR, Pyke KA, Micol JL - Mutations in the RETICULATA gene dramatically alter internal architecture but have little effect on overall organ shape in Arabidopsis leaves
Visit the PubMed ArticleSubsequently, during leaf development, the pattern of PPD expression followed the tip-to-base progression of the general proliferative arrest front before becoming restricted to developing vascular traces and eventually declining in expression in the same pattern as the arrest of procambial cell division
White DW - PEAPOD regulates lamina size and curvature in Arabidopsis
Visit the PubMed ArticleDuring leaf development, PPD expression was first detected at the tip of the developing leaf, distal to the general proliferative cell-cycle arrest front and coincident with the initiation of trichome and margin cell development (Fig. 4 A and D Inset). This expression appeared to exist initially in all cells in the newly formed DMC zone and was not restricted to meristemoid cells
White DW - PEAPOD regulates lamina size and curvature in Arabidopsis
Visit the PubMed ArticleSubsequently, during leaf development, the pattern of PPD expression followed the tip-to-base progression of the general proliferative arrest front before becoming restricted to developing vascular traces and eventually declining in expression in the same pattern as the arrest of procambial cell division
White DW - PEAPOD regulates lamina size and curvature in Arabidopsis
Visit the PubMed ArticleDuring leaf development, PPD expression was first detected at the tip of the developing leaf, distal to the general proliferative cell-cycle arrest front and coincident with the initiation of trichome and margin cell development (Fig. 4 A and D Inset). This expression appeared to exist initially in all cells in the newly formed DMC zone and was not restricted to meristemoid cells
White DW - PEAPOD regulates lamina size and curvature in Arabidopsis
Visit the PubMed ArticleDR5-GUS reporter was not expressed in the veins of 21-day-old Col-0 and hve-3/hve-3 leaves from the first, third and seventh nodes
Alonso-Peral MM, Candela H, del Pozo JC, Martínez-Laborda A, Ponce MR, Micol JL - The HVE/CAND1 gene is required for the early patterning of leaf venation in Arabidopsis
Visit the PubMed ArticleATHB-8-GUS ... the expression of the reporter revealed a much simpler GUS pattern in hve-3/hve-3 leaves than in wild-type leaves of the same age (compare Fig. 5A with 5I, 5B with 5J, 5C with 5K, and 5D with 5L). The GUS pattern was restricted to the fully differentiated venation pattern of the mutant, which lacked most tertiary and higher order veins. The absence of GUS staining at the sites where the veins normally differentiate indicates that ATHB-8-GUS expression is dependent on the earlier acquisition of vascular fate at specific locations within the leaf. Identical results were obtained in comparisons of Ws-2 with hve-1/hve-1
Alonso-Peral MM, Candela H, del Pozo JC, Martínez-Laborda A, Ponce MR, Micol JL - The HVE/CAND1 gene is required for the early patterning of leaf venation in Arabidopsis
Visit the PubMed ArticleCol-0 RNA was extracted from assorted organs and RT-PCR amplified, and HVE was found to be expressed in every organ studied
Alonso-Peral MM, Candela H, del Pozo JC, Martínez-Laborda A, Ponce MR, Micol JL - The HVE/CAND1 gene is required for the early patterning of leaf venation in Arabidopsis
Visit the PubMed Articlepostembryonic spatial expression pattern ... HVE ... GUS staining was mostly confined to the leaf vasculature in 21-day-old plants, indicating that HVE expression changes dynamically throughout leaf development (Fig. 4C). For cotyledons and vegetative leaves, the expression in mesophyll cells was apparently more intense at the beginning of leaf expansion, disappearing progressively as the leaves grew (Fig. 4D,E-H,P). Closer inspection revealed that the gene was expressed before xylem differentiation in developing veins at the basal actively dividing region of rosette leaves (Fig. 4R) and, after the differentiation of tracheary elements, in the living cells of the vascular bundles (Fig. 4Q
Alonso-Peral MM, Candela H, del Pozo JC, Martínez-Laborda A, Ponce MR, Micol JL - The HVE/CAND1 gene is required for the early patterning of leaf venation in Arabidopsis
Visit the PubMed ArticleTo further define the postembryonic spatial expression pattern of HVE/CAND1, we characterized the wild-type expression pattern (Fig. 4) of a CAND1 promoter-GUS fusion (PETA2-GUS) that includes 2.7 kb of upstream sequence (Chuang et al., 2004). Consistent with the pleiotropic phenotype of loss-of-function hve mutants and with our semi-quantitative RT-PCR results, the gene was found almost ubiquitously expressed in aerial and underground organs of 10-day-old plants (Fig. 4A,B). At this stage, the gene was widely expressed in rosette leaves, at the highest level in the vasculature
Alonso-Peral MM, Candela H, del Pozo JC, Martínez-Laborda A, Ponce MR, Micol JL - The HVE/CAND1 gene is required for the early patterning of leaf venation in Arabidopsis
Visit the PubMed ArticleDR5-GUS reporter ... was in the leaf margin, the hydathodes and some mesophyll regions. For actively developing leaves of the 9th and upper nodes, by contrast, the DR5-GUS reporter was expressed at the tip, hydathodes, and tertiary and higher order veins of the proximal regions of the lamina in the wild type, but only at the tip in hve-3/hve-3 leaves
Alonso-Peral MM, Candela H, del Pozo JC, Martínez-Laborda A, Ponce MR, Micol JL - The HVE/CAND1 gene is required for the early patterning of leaf venation in Arabidopsis
Visit the PubMed ArticleATHB-8-GUS reporter ... in ... wild-type leaves, the expression of the reporter uncovered a complex, reticulate pattern consisting of mature and differentiating vascular strands
Alonso-Peral MM, Candela H, del Pozo JC, Martínez-Laborda A, Ponce MR, Micol JL - The HVE/CAND1 gene is required for the early patterning of leaf venation in Arabidopsis
Visit the PubMed ArticleWe observed a similar expression profile of ectopic BP but not STM in leaves of a dominant Aux/IAA17 mutant, axr3-1
Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis
Visit the PubMed Articlepolar localisation of PIN1:GFP in margin cells of plants grown on MS medium (arrowheads, Fig. 3G) were abolished in NPA-grown plants
Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis
Visit the PubMed ArticleIn comparison with wild-type leaves ... DR5::GUS expression was repressed in the distal lamina and concentrated in developing lobes of 35S::BP leaves
Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis
Visit the PubMed ArticleThis ectopic BP expression in axr1 leaves was not accompanied by a reduction in AS1 transcripts
Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis
Visit the PubMed ArticleBP::GUS ... expression was observed in the serration tips of axr1 leaves
Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis
Visit the PubMed Articlewe observed ectopic BP::GUS expression in the leaves of plants treated with the auxin transport inhibitors 1-N-naphthylphthalamic acid (NPA) or 2,3,5triiodobenzoic acid (TIBA
Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis
Visit the PubMed ArticleWe observed inappropriate expression of the KNOX gene BP but not SHOOTMERISTEMLESS (STM) in axr1 mutant leaves, although the level of BP expression was substantially higher in as1 than in axr1 leaves
Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis
Visit the PubMed ArticleWe observed inappropriate expression of the KNOX gene BP ... in axr1 mutant leaves
Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis
Visit the PubMed Articlein FIL>>BP plants ... NPA treatment ... prevented the generation of PIN1-directed auxin maxima in the leaf margin
Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis
Visit the PubMed ArticleIn comparison with wild-type leaves ... PIN1::GUS ... expression was repressed in the distal lamina and concentrated in developing lobes of 35S::BP leaves
Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis
Visit the PubMed ArticleWe observed inappropriate expression of the KNOX gene BP but not SHOOTMERISTEMLESS (STM) in axr1
Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis
Visit the PubMed ArticlePIN1:GFP expression in initiating leaflets of FIL>>BP leaves was indistinguishable from that of wild type; however, expression persisted as leaflets developed in FIL>>BP leaves (arrows Fig. 4G), suggesting that KNOX activity in the leaf prevents the normal basipetal displacement of PIN1:GFP expression maxima, correlating with prolonged localised growth and leaflet formation
Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis
Visit the PubMed ArticleBP::GUS was absent from wild-type leaves
Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis
Visit the PubMed ArticlePIN1:GFP expression in initiating leaflets of FIL>>BP leaves was indistinguishable from that of wild type; however, expression persisted as leaflets developed in FIL>>BP leaves (arrows Fig. 4G), suggesting that KNOX activity in the leaf prevents the normal basipetal displacement of PIN1:GFP expression maxima, correlating with prolonged localised growth and leaflet formation. BP-induced alterations in PIN1:GFP expression were mirrored by similar alterations in expression of the AINTEGUMENTA (ANT) gene
Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis
Visit the PubMed ArticleDR5rev::GFP was localised in the tips of initiating serrations
Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis
Visit the PubMed Articlein pin1 leaves, relative to wild type ... AS1 transcript levels were unaltered
Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis
Visit the PubMed ArticleBP::GUS ... was observed ... in the ... midvein ... of as1 leaves
Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis
Visit the PubMed ArticleWe observed inappropriate expression of the KNOX gene BP ... expression was substantially higher in as1 than in axr1 leaves
Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis
Visit the PubMed ArticleWe observed a similar expression profile of ectopic BP ... in leaves of a dominant Aux/IAA17 mutant, axr3-1
Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis
Visit the PubMed Articlepolar expression of PIN1:GFP in the epidermis
Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis
Visit the PubMed ArticleBP::GUS ... expression was observed ... in the petiole, midvein and serration tips of as1 leaves
Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis
Visit the PubMed Articlein the leaves of plants treated with the auxin transport inhibitors 1-N-naphthylphthalamic acid (NPA) or 2,3,5triiodobenzoic acid (TIBA ... STM::GUS expression was unaltered
Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis
Visit the PubMed ArticleBP::GUS ... expression was observed ... in the petiole ... of as1 leaves
Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis
Visit the PubMed ArticleWe observed misexpression of BP transcripts in pin1 leaves, relative to wild type
Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis
Visit the PubMed Articlein as1 ... leaves ... This ectopic BP expression ... was not accompanied by a reduction in AS1 transcripts
Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis
Visit the PubMed ArticleAlthough BP::GUS was absent from wild-type leaves (Fig. 1R), expression was observed in the serration tips of axr1 leaves (Fig. 1S), and in the petiole, midvein and serration tips of as1 leaves (Fig. 1T). However, the pattern of BP::GUS expression in as1;axr1 double mutant leaves was different than that of either single mutant, being sharply localised to margin cells in the sinus of every lobe from an early stage in leaf development (arrows, Fig. 1U,V), correlating with the ectopic initiation of stipules
Hay A, Barkoulas M, Tsiantis M - ASYMMETRIC LEAVES1 and auxin activities converge to repress BREVIPEDICELLUS expression and promote leaf development in Arabidopsis
Visit the PubMed ArticleFAS1 transcript ... fas1-1 ... is a null allele
Exner V, Taranto P, Schönrock N, Gruissem W, Hennig L - Chromatin assembly factor CAF-1 is required for cellular differentiation during plant development
Visit the PubMed Articlesome FAS1 transcript is made in the fas1-4 insertion mutant (see Fig. S1 in the supplementary material), demonstrating that fas1-1 but not fas1-4 is a null allele
Exner V, Taranto P, Schönrock N, Gruissem W, Hennig L - Chromatin assembly factor CAF-1 is required for cellular differentiation during plant development
Visit the PubMed Articlefas2 alleles appear to be null
Exner V, Taranto P, Schönrock N, Gruissem W, Hennig L - Chromatin assembly factor CAF-1 is required for cellular differentiation during plant development
Visit the PubMed Articlethe Atebp1(RNAi) plants had a ... reduction of the CDKB1;1
Horváth BM, Magyar Z, Zhang Y, Hamburger AW, Bakó L, Visser RG, Bachem CW, Bögre L - EBP1 regulates organ size through cell growth and proliferation in plants
Visit the PubMed ArticleWe found that the overexpression of EBP1 in Arabidopsis cells dose-dependently reduced the endogenous RBR1 protein
Horváth BM, Magyar Z, Zhang Y, Hamburger AW, Bakó L, Visser RG, Bachem CW, Bögre L - EBP1 regulates organ size through cell growth and proliferation in plants
Visit the PubMed ArticleWe found that silencing of AtEBP1 completely abolished the activation of RNR2 promoter by auxin (Figure 5E), indicating that AtEBP1 is required for the auxin-dependent promoter activity
Horváth BM, Magyar Z, Zhang Y, Hamburger AW, Bakó L, Visser RG, Bachem CW, Bögre L - EBP1 regulates organ size through cell growth and proliferation in plants
Visit the PubMed ArticleIn contrast, the Atebp1(RNAi) plants had a consistently elevated RBR1 protein accumulation
Horváth BM, Magyar Z, Zhang Y, Hamburger AW, Bakó L, Visser RG, Bachem CW, Bögre L - EBP1 regulates organ size through cell growth and proliferation in plants
Visit the PubMed Articleexpression levels of ... CYP90D1 (a ROT3 homolog ... not altered in ... lng1-3 lng2-1
Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis
Visit the PubMed Articlewe determined the expression patterns of these genes in promoter:GUS transgenic plants. Promoter sequences (∼2 kb) from the LNG1 and LNG2 genes were used to drive expression of GUS. The ProLNG1:GUS ... expressed in ... leaf blades
Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis
Visit the PubMed Articlewe determined the expression patterns of these genes in promoter:GUS transgenic plants. Promoter sequences (∼2 kb) from the LNG1 and LNG2 genes were used to drive expression of GUS. The ProLNG1:GUS ... expressed in various parts of adult transgenic plants, including the petioles
Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis
Visit the PubMed Articlewe determined the expression patterns of these genes in promoter:GUS transgenic plants. Promoter sequences (∼2 kb) from the LNG1 and LNG2 genes were used to drive expression of GUS ... ProLNG2:GUS ... expressed in ... petioles
Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis
Visit the PubMed Articlelng1-1D ... T-DNA insertion at 5477 bp upstream of At5g15880 ... Northern blot analysis indicated that the expression of one gene (At5g15880) was higher in mutant plants compared with wild type
Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis
Visit the PubMed Articleexpression levels of ... AN ... not altered in lng1-1D
Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis
Visit the PubMed ArticleLNG2 expression levels were unchanged in the rot3-1 mutant
Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis
Visit the PubMed ArticleProLNG1:GUS ... GUS staining ... stronger staining in the veins
Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis
Visit the PubMed Articleexpression levels of ... ATHB13 were not altered in lng1-1D
Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis
Visit the PubMed ArticleProLNG2:GUS ... In leaves, GUS staining was observed in all parts
Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis
Visit the PubMed Articleexpression levels of ROT3 ... not altered in ... lng1-3 lng2-1
Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis
Visit the PubMed ArticleProLNG2:GUS ... GUS was strongly expressed in and at the base of the petioles
Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis
Visit the PubMed ArticleProLNG1:GUS ... GUS was strongly expressed in and at the base of the petioles
Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis
Visit the PubMed Articleexpression levels of ROT3 ... not altered in lng1-1D
Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis
Visit the PubMed ArticleProLNG1:GUS ... In leaves, GUS staining was observed in all parts
Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis
Visit the PubMed ArticleThe GFP-LNG1 fusion protein showed similar localizations in ... nucleus
Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis
Visit the PubMed Articleexpression levels of ... AN ... not altered in ... lng1-3 lng2-1
Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis
Visit the PubMed Articleexpression levels of ... ATHB13 were not altered in ... lng1-3 lng2-1
Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis
Visit the PubMed Articleexpression levels of ... CYP90D1 (a ROT3 homolog ... not altered in lng1-1D
Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis
Visit the PubMed ArticleThe GFP-LNG1 fusion protein showed similar localizations in ... the cytosol
Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis
Visit the PubMed ArticleLNG1 ... expression levels were unchanged in the rot3-1 mutant
Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis
Visit the PubMed Articlewe determined the expression patterns of these genes in promoter:GUS transgenic plants. Promoter sequences (∼2 kb) from the LNG1 and LNG2 genes were used to drive expression of GUS ... ProLNG2:GUS ... expressed in ... leaf blades
Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis
Visit the PubMed ArticleProLNG2:GUS ... GUS staining ... stronger staining in the veins
Lee YK, Kim GT, Kim IJ, Park J, Kwak SS, Choi G, Chung WI - LONGIFOLIA1 and LONGIFOLIA2, two homologous genes, regulate longitudinal cell elongation in Arabidopsis
Visit the PubMed ArticleTo obtain further insights into the potential role of farnesylation for AtNAP1;1 subcellular localization, GFP-AtNAP1;1 and GFP-AtNAP1;1C369S were stably expressed in transgenic tobacco BY-2 cells (Fig. 2B). GFP-AtNAP1;1 was localized in both the cytoplasm and the nucleoplasm of interphase cells. During mitosis, GFP-AtNAP1;1 was colocalized with the phragmoplast in telophase
Galichet A, Gruissem W - Developmentally controlled farnesylation modulates AtNAP1;1 function in cell proliferation and cell expansion during Arabidopsis leaf development
Visit the PubMed ArticleTo investigate the subcellular localization of AtNAP1;1, GFP-AtNAP1;1 and GFP-AtNAP1;1C369S were transiently expressed in onion (Allium cepa) epidermal cells. GFP alone was distributed uniformly in the nucleus and the cytoplasm, whereas GFP-AtNAP1;1 and GFP-AtNAP1;1C369S were restricted to the cytoplasm
Galichet A, Gruissem W - Developmentally controlled farnesylation modulates AtNAP1;1 function in cell proliferation and cell expansion during Arabidopsis leaf development
Visit the PubMed ArticleBOLpromoter-GUS construct was used to study further the temporal and spatial pattern of expression ... Five days after germination, seedlings showed mild staining at the SAM
Marsch-Martinez N, Greco R, Becker JD, Dixit S, Bergervoet JH, Karaba A, de Folter S, Pereira A - BOLITA, an Arabidopsis AP2/ERF-like transcription factor that affects cell expansion and proliferation/differentiation pathways
Visit the PubMed Articlebol- D ... Arabidopsis leaves ... many calcium or calmodulin binding genes, including the calcium dependent protein kinase CPK32, were also downregulated
Marsch-Martinez N, Greco R, Becker JD, Dixit S, Bergervoet JH, Karaba A, de Folter S, Pereira A - BOLITA, an Arabidopsis AP2/ERF-like transcription factor that affects cell expansion and proliferation/differentiation pathways
Visit the PubMed Articlebol- D ... Arabidopsis leaves ... The auxin induced genes TCH3 and PBP1 which contain calcium binding motives and interact in a calcium dependent manner with the PINOID kinase, a key component in auxin signaling, were also repressed
Marsch-Martinez N, Greco R, Becker JD, Dixit S, Bergervoet JH, Karaba A, de Folter S, Pereira A - BOLITA, an Arabidopsis AP2/ERF-like transcription factor that affects cell expansion and proliferation/differentiation pathways
Visit the PubMed ArticleBOLpromoter-GUS construct was used to study further the temporal and spatial pattern of expression ... In the first 2 days after germination, staining occurred at the shoot apical meristem
Marsch-Martinez N, Greco R, Becker JD, Dixit S, Bergervoet JH, Karaba A, de Folter S, Pereira A - BOLITA, an Arabidopsis AP2/ERF-like transcription factor that affects cell expansion and proliferation/differentiation pathways
Visit the PubMed ArticleBOLpromoter-GUS construct was used to study further the temporal and spatial pattern of expression ... Emerging leaves from older seedlings stained first at the tip and later at separated spots at the leaf periphery (hydatodes)
Marsch-Martinez N, Greco R, Becker JD, Dixit S, Bergervoet JH, Karaba A, de Folter S, Pereira A - BOLITA, an Arabidopsis AP2/ERF-like transcription factor that affects cell expansion and proliferation/differentiation pathways
Visit the PubMed ArticleBOLpromoter-GUS construct was used to study further the temporal and spatial pattern of expression ... Five days after germination, seedlings showed ... intense staining at leaf primordia
Marsch-Martinez N, Greco R, Becker JD, Dixit S, Bergervoet JH, Karaba A, de Folter S, Pereira A - BOLITA, an Arabidopsis AP2/ERF-like transcription factor that affects cell expansion and proliferation/differentiation pathways
Visit the PubMed ArticleBOLpromoter-GUS construct was used to study further the temporal and spatial pattern of expression ... In the first 2 days after germination, staining occurred ... at the distal regions of the cotyledons
Marsch-Martinez N, Greco R, Becker JD, Dixit S, Bergervoet JH, Karaba A, de Folter S, Pereira A - BOLITA, an Arabidopsis AP2/ERF-like transcription factor that affects cell expansion and proliferation/differentiation pathways
Visit the PubMed ArticleRT-PCR experiments were then performed to assess expression of AP2/ERF gene At1g24590, representing the best candidate based on the position of the insert in the activation tag mutant and the nature of the gene itself. While in wild type plants the presence of its transcript was detected only in flower buds, in the bol-D mutant hyper-accumulation of this transcript occurred in roots, rosette and cauline leaves, flowers buds and open flowers
Marsch-Martinez N, Greco R, Becker JD, Dixit S, Bergervoet JH, Karaba A, de Folter S, Pereira A - BOLITA, an Arabidopsis AP2/ERF-like transcription factor that affects cell expansion and proliferation/differentiation pathways
Visit the PubMed Articlebol- D ... Arabidopsis leaves ... The auxin induced genes TCH3 and PBP1 which contain calcium binding motives and interact in a calcium dependent manner with the PINOID kinase, a key component in auxin signaling, were also repressed
Marsch-Martinez N, Greco R, Becker JD, Dixit S, Bergervoet JH, Karaba A, de Folter S, Pereira A - BOLITA, an Arabidopsis AP2/ERF-like transcription factor that affects cell expansion and proliferation/differentiation pathways
Visit the PubMed ArticleProCUC2:GUS reporter ... In larger leaves, GUS staining was restricted to the proximal part of the leaves, in which the teeth were still forming
Nikovics K, Blein T, Peaucelle A, Ishida T, Morin H, Aida M, Laufs P - The balance between the MIR164A and CUC2 genes controls leaf margin serration in Arabidopsis
Visit the PubMed ArticleProMIR164A-2.1:GUS construct ... later appeared ... in the vasculature
Nikovics K, Blein T, Peaucelle A, Ishida T, Morin H, Aida M, Laufs P - The balance between the MIR164A and CUC2 genes controls leaf margin serration in Arabidopsis
Visit the PubMed ArticleGUS staining was observed at the point of insertion of the primordium into the meristem in plants carrying the ProMIR164A-2.1:GUS construct
Nikovics K, Blein T, Peaucelle A, Ishida T, Morin H, Aida M, Laufs P - The balance between the MIR164A and CUC2 genes controls leaf margin serration in Arabidopsis
Visit the PubMed ArticleIn mir164a-4 leaves ... At5g61430 transcript levels were two to three times higher than those in the wild type
Nikovics K, Blein T, Peaucelle A, Ishida T, Morin H, Aida M, Laufs P - The balance between the MIR164A and CUC2 genes controls leaf margin serration in Arabidopsis
Visit the PubMed Articleno ectopic KNAT1 ... expression was observed in the serrated CUC2g-m4 leaves
Nikovics K, Blein T, Peaucelle A, Ishida T, Morin H, Aida M, Laufs P - The balance between the MIR164A and CUC2 genes controls leaf margin serration in Arabidopsis
Visit the PubMed ArticleIn mir164a-4 leaves ... At5g07680 ... transcript levels were two to three times higher than those in the wild type
Nikovics K, Blein T, Peaucelle A, Ishida T, Morin H, Aida M, Laufs P - The balance between the MIR164A and CUC2 genes controls leaf margin serration in Arabidopsis
Visit the PubMed ArticleIn mir164a-4 leaves ... CUC2 transcript levels were about five times higher than those in the wild type
Nikovics K, Blein T, Peaucelle A, Ishida T, Morin H, Aida M, Laufs P - The balance between the MIR164A and CUC2 genes controls leaf margin serration in Arabidopsis
Visit the PubMed Articleno ectopic ... KNAT2 expression was observed in the serrated CUC2g-m4 leaves
Nikovics K, Blein T, Peaucelle A, Ishida T, Morin H, Aida M, Laufs P - The balance between the MIR164A and CUC2 genes controls leaf margin serration in Arabidopsis
Visit the PubMed ArticleProMIR164A-2.1:GUS ... later appeared at the tip of the organ
Nikovics K, Blein T, Peaucelle A, Ishida T, Morin H, Aida M, Laufs P - The balance between the MIR164A and CUC2 genes controls leaf margin serration in Arabidopsis
Visit the PubMed ArticleProMIR164A-2.1:GUS construct ... In small primordia, GUS activity was observed at the margins
Nikovics K, Blein T, Peaucelle A, Ishida T, Morin H, Aida M, Laufs P - The balance between the MIR164A and CUC2 genes controls leaf margin serration in Arabidopsis
Visit the PubMed ArticleProCUC2:GUS reporter ... During leaf development, GUS activity was observed in the margins of the developing primordium
Nikovics K, Blein T, Peaucelle A, Ishida T, Morin H, Aida M, Laufs P - The balance between the MIR164A and CUC2 genes controls leaf margin serration in Arabidopsis
Visit the PubMed ArticleIn mir164a-4 leaves, NAC1 ... transcript levels ... were two to three times higher than those in the wild type
Nikovics K, Blein T, Peaucelle A, Ishida T, Morin H, Aida M, Laufs P - The balance between the MIR164A and CUC2 genes controls leaf margin serration in Arabidopsis
Visit the PubMed ArticleA transcriptional reporter for MUTE is expressed ... at lower levels in ... guard cells
MacAlister CA, Ohashi-Ito K, Bergmann DC - Transcription factor control of asymmetric cell divisions that establish the stomatal lineage
Visit the PubMed ArticleA transcriptional green fluorescent protein (GFP) reporter (SPCHpro::nucGFP) and a rescuing translational reporter (SPCHpro::SPCH�GFP) are expressed in a subset of epidermal cells that lack overt signs of differentiation ... Expression commences in cotyledons coincident with the onset of postembryonic cell division
MacAlister CA, Ohashi-Ito K, Bergmann DC - Transcription factor control of asymmetric cell divisions that establish the stomatal lineage
Visit the PubMed ArticleSPCH is expressed in the developing leaf epidermis
MacAlister CA, Ohashi-Ito K, Bergmann DC - Transcription factor control of asymmetric cell divisions that establish the stomatal lineage
Visit the PubMed Articlespch-1 plants also fail to express TMM
MacAlister CA, Ohashi-Ito K, Bergmann DC - Transcription factor control of asymmetric cell divisions that establish the stomatal lineage
Visit the PubMed ArticleIn older organs, SPCHpro::SPCH�GFP expression continues to be restricted to small cells in the epidermis (Fig. 2d), including cells that have recently divided next to stomatal lineage cells
MacAlister CA, Ohashi-Ito K, Bergmann DC - Transcription factor control of asymmetric cell divisions that establish the stomatal lineage
Visit the PubMed ArticleSPCHpro::nucGFP persists in stomatal lineage cells
MacAlister CA, Ohashi-Ito K, Bergmann DC - Transcription factor control of asymmetric cell divisions that establish the stomatal lineage
Visit the PubMed ArticleA transcriptional reporter for MUTE is expressed ... at lower levels in GMCs
MacAlister CA, Ohashi-Ito K, Bergmann DC - Transcription factor control of asymmetric cell divisions that establish the stomatal lineage
Visit the PubMed ArticleSPCH expression is often found in two neighbouring cells�a pattern consistent with expression in the dividing cell population
MacAlister CA, Ohashi-Ito K, Bergmann DC - Transcription factor control of asymmetric cell divisions that establish the stomatal lineage
Visit the PubMed ArticleA transcriptional reporter for MUTE is expressed strongly in meristemoids
MacAlister CA, Ohashi-Ito K, Bergmann DC - Transcription factor control of asymmetric cell divisions that establish the stomatal lineage
Visit the PubMed ArticleFAMA is expressed in ... immature guard cells
Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata
Visit the PubMed ArticleTo examine ... subcellular localization, we used ... the translational fusion of a full-length MUTE protein and GFP (MUTE ... MUTE�GFP ... No GFP signal was detected in ... newly formed meristemoids
Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata
Visit the PubMed ArticleSPCH promoter activity (SPCH::GUS) was observed broadly in the protoderm of leaf primordia
Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata
Visit the PubMed ArticleThe ... arrested mute meristemoids expressed high levels ... ERL1::GUS, a reporter b-glucuronidase driven by the ERL1 promoter6
Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata
Visit the PubMed ArticleThe arrested mute meristemoids expressed high levels of TMM::TMM�GFP, a translational fusion of TMM with a green fluorescent protein
Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata
Visit the PubMed ArticleBubble-like� clusters of small cells ... observed in the epidermis of ... mute; erecta; erl1; erl2 ... expressed TMM::GUS ... at high levels
Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata
Visit the PubMed ArticleTo examine promoter activity ... we used the native MUTE promoter to drive expression of the GUS reporter (MUTE::GUS ... MUTE::GUS expression ... residual activity in ... immature guard cells
Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata
Visit the PubMed ArticleIn ... ectopic overexpression of SPCH by the CaMV35S promoter ... numerous small cells ... strongly express TMM::TMM�GFP, indicating that they probably have the identity of stomatal lineage cells
Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata
Visit the PubMed ArticleERL1 mark stomatal lineage cells ... ERL1::GUS, a reporter b-glucuronidase driven by the ERL1 promoter6
Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata
Visit the PubMed ArticleLikewise, the arrested meristemoids in mute did not express the mature-guard-cell-specific GFP marker E994
Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata
Visit the PubMed ArticleTMM ... mark stomatal lineage cells with the highest expression in meristemoids ... TMM::TMM�GFP
Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata
Visit the PubMed ArticleTo examine promoter activity ... we used the native MUTE promoter to drive expression of the GUS reporter (MUTE::GUS ... MUTE::GUS expression was highest in a subset of meristemoids that have undergone a few rounds of asymmetric division
Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata
Visit the PubMed ArticleIn the wild-type leaf epidermis, FAMA�GFP was ... not in meristemoids
Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata
Visit the PubMed ArticleFAMA::FAMA�GFP, a translational fusion of GFP and FAMA ... In the wild-type leaf epidermis, FAMA�GFP was specifically detected in the nuclei of GMCs
Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata
Visit the PubMed ArticleTo examine ... subcellular localization, we used ... the translational fusion of a full-length MUTE protein and GFP (MUTE::MUTE�GFP ... The MUTE�GFP fusion protein was localized in the nuclei ... nuclei of ... recently transitioned GMCs
Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata
Visit the PubMed ArticleBubble-like� clusters of small cells ... observed in the epidermis of ... mute; erecta; erl1; erl2 ... expressed ... ERL1::GUS at high levels
Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata
Visit the PubMed ArticleIn the wild-type leaf epidermis, FAMA�GFP was specifically detected in the nuclei ... early, immature guard cells
Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata
Visit the PubMed ArticleTo examine ... subcellular localization, we used ... the translational fusion of a full-length MUTE protein and GFP (MUTE::MUTE�GFP ... No GFP signal was detected in ... immature ... e and mature guard cells
Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata
Visit the PubMed ArticleFAMA is expressed in GMCs
Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata
Visit the PubMed ArticleBubble-like� clusters of small cells ... observed in the epidermis of mute; tmm ... expressed ... ERL1::GUS at high levels
Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata
Visit the PubMed ArticleIn mute, no FAMA�GFP expression was detected
Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata
Visit the PubMed ArticleBubble-like� clusters of small cells ... observed in the epidermis of mute; tmm ... expressed TMM::GUS ... at high levels
Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata
Visit the PubMed ArticleTMM ... mark stomatal lineage cells with the highest expression in meristemoids ... TMM::TMM�GFP
Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata
Visit the PubMed ArticleTo examine ... subcellular localization, we used ... the translational fusion of a full-length MUTE protein and GFP (MUTE::MUTE�GFP ... The MUTE�GFP fusion protein was localized in the nuclei of a subset of meristemoids that have undergone a few rounds of asymmetric division
Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata
Visit the PubMed ArticleSPCH promoter activity (SPCH::GUS) was ... localized in stomatal lineage cells later in development
Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata
Visit the PubMed ArticleThe spch mutant does not express MUTE transcripts
Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata
Visit the PubMed ArticleTo examine promoter activity ... we used the native MUTE promoter to drive expression of the GUS reporter ... residual activity in GMC
Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata
Visit the PubMed ArticleERL1 mark stomatal lineage cells with the highest expression in meristemoids ... ERL1::GUS, a reporter b-glucuronidase driven by the ERL1 promoter6
Pillitteri LJ, Sloan DB, Bogenschutz NL, Torii KU - Termination of asymmetric cell division and differentiation of stomata
Visit the PubMed ArticleDuring leaf development ... expression of ... AtML1∷KRP4 was observed ... in the abaxial epidermis
Bemis SM, Torii KU - Autonomy of cell proliferation and developmental programs during Arabidopsis aboveground organ morphogenesis
Visit the PubMed Articleno difference was observed in CLV3 ... expression levels ... between transgenic and Ler plants
Bemis SM, Torii KU - Autonomy of cell proliferation and developmental programs during Arabidopsis aboveground organ morphogenesis
Visit the PubMed Articleno difference was observed in CLV3 expression ... patterns between transgenic and Ler plants
Bemis SM, Torii KU - Autonomy of cell proliferation and developmental programs during Arabidopsis aboveground organ morphogenesis
Visit the PubMed ArticleWe ... investigated expression patterns in the transgenic plants expressing ... AtML1∷KRP4 ... L1-layer-specific accumulation of ... KRP4 transcripts was detected in the ... leaf primordia
Bemis SM, Torii KU - Autonomy of cell proliferation and developmental programs during Arabidopsis aboveground organ morphogenesis
Visit the PubMed ArticleWe ... investigated expression patterns in the transgenic plants expressing ... AtML1∷KRP4 ... L1-layer-specific accumulation of ... KRP4 transcripts was detected in the SAM
Bemis SM, Torii KU - Autonomy of cell proliferation and developmental programs during Arabidopsis aboveground organ morphogenesis
Visit the PubMed ArticleWe ... investigated expression patterns in the transgenic plants expressing AtML1∷KRP1/ICK1 ... L1-layer-specific accumulation of KRP1/ICK1 ... transcripts was detected in the SAM
Bemis SM, Torii KU - Autonomy of cell proliferation and developmental programs during Arabidopsis aboveground organ morphogenesis
Visit the PubMed Articleno difference was observed in CLV3 expression ... patterns between transgenic and Ler plants
Bemis SM, Torii KU - Autonomy of cell proliferation and developmental programs during Arabidopsis aboveground organ morphogenesis
Visit the PubMed ArticleWe ... investigated expression patterns in the transgenic plants expressing AtML1∷KRP1/ICK1 ... L1-layer-specific accumulation of KRP1/ICK1 ... transcripts was detected in the ... leaf primordia
Bemis SM, Torii KU - Autonomy of cell proliferation and developmental programs during Arabidopsis aboveground organ morphogenesis
Visit the PubMed Articleexpression patterns of endogenous ... KRP4 transcripts were examined in non-transformed Ler plants ... KRP4 was detected in the SAM
Bemis SM, Torii KU - Autonomy of cell proliferation and developmental programs during Arabidopsis aboveground organ morphogenesis
Visit the PubMed Articleexpression patterns of endogenous KRP1/ICK1 ... transcripts were examined in non-transformed Ler plants. We did not detect significant expression of KRP1/ICK1 beyond the level of the sense control, perhaps due to its uniform expression in vacuolated, mature cells
Bemis SM, Torii KU - Autonomy of cell proliferation and developmental programs during Arabidopsis aboveground organ morphogenesis
Visit the PubMed ArticleAtML1∷KRP4 transgenic lines, cells in the inner layers did not exhibit high accumulation of ... KRP4 beyond the levels of endogenous transcripts
Bemis SM, Torii KU - Autonomy of cell proliferation and developmental programs during Arabidopsis aboveground organ morphogenesis
Visit the PubMed ArticleDuring leaf development ... expression of AtML1∷KRP1/ICK1 ... was observed in the adaxial epidermis
Bemis SM, Torii KU - Autonomy of cell proliferation and developmental programs during Arabidopsis aboveground organ morphogenesis
Visit the PubMed ArticleAtML1∷KRP1/ICK1 ... transgenic lines, cells in the inner layers did not exhibit high accumulation of KRP1/ICK1 ... beyond the levels of endogenous transcripts
Bemis SM, Torii KU - Autonomy of cell proliferation and developmental programs during Arabidopsis aboveground organ morphogenesis
Visit the PubMed ArticleDuring leaf development ... expression of AtML1∷KRP1/ICK1 ... was observed ... in the abaxial epidermis
Bemis SM, Torii KU - Autonomy of cell proliferation and developmental programs during Arabidopsis aboveground organ morphogenesis
Visit the PubMed ArticleDuring leaf development ... expression of ... AtML1∷KRP4 was observed in the adaxial epidermis
Bemis SM, Torii KU - Autonomy of cell proliferation and developmental programs during Arabidopsis aboveground organ morphogenesis
Visit the PubMed Articleno difference was observed in CLV3 expression levels ... between transgenic and Ler plants
Bemis SM, Torii KU - Autonomy of cell proliferation and developmental programs during Arabidopsis aboveground organ morphogenesis
Visit the PubMed ArticleTwo upregulated genes were involved in callose synthesis during cell plate formation: ENDOXYLOGLUCAN TRANSFERASE (DECOY) and CALLOSE SYNTHASE1, both of which are implicated in cell wall/cell plate formation (Table 3 ... in hub1-1
Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth
Visit the PubMed ArticleOther categories downregulated in hub1-1 comprised genes involved in carboxylic acid metabolism, fatty acid metabolism, lipid biosynthesis, vitamin biosynthesis, cofactor biosynthesis, chlorophyll biosynthesis, transfer RNA metabolism, photosynthesis, and protein targeted to chloroplast
Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth
Visit the PubMed Articlethe E2F dimerization partner (DPa) transcription factor gene were upregulated ... in hub1-1
Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth
Visit the PubMed Articlesome overex- pressed genes in hub1-1 were related to meristem development, such as the homeodomain genes ... SHOOT MERISTEMLESS
Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth
Visit the PubMed Articlethe analysis of upregulated genes also identified GO categories related to cytokinesis ... in hub1-1
Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth
Visit the PubMed ArticleA significantly high number of genes differentially ex- pressed in hub1-1 were related to cell cycle (24 genes) and cytokinesis (31 genes)
Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth
Visit the PubMed ArticleAmong the downregulated genes in hub1-1, cell fate specifi- cation, histone phosphorylation, regulation of progression through cell cycle, mitotic cell cycle, and microtubule-based movement genes were significantly overrepresented. For cellular compo- nents, the underexpressed genes in hub1-1 were significantly related to myosin, spindle, microtubule, and phragmoplast (data not shown). Among the 82 genes with a peak expression in mitosis (Menges et al., 2005), 66 were significantly downregulated in the hub1-1 mutant, of which 37 genes had a known function in mitosis
Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth
Visit the PubMed ArticleTwo upregulated genes were involved in callose synthesis during cell plate formation: ENDOXYLOGLUCAN TRANSFERASE (DECOY) and CALLOSE SYNTHASE1, both of which are implicated in cell wall/cell plate formation (Table 3 ... in hub1-1
Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth
Visit the PubMed Articlethe SIAMESE (SIM) gene, with a function in endoreduplication during trichome formation on leaves ... upregulated ... in hub1-1
Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth
Visit the PubMed Articlesome overex- pressed genes in hub1-1 were related to meristem development, such as the homeodomain genes ... KNAT6
Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth
Visit the PubMed ArticleA total of 13 homeotic genes had an altered expression in hub1-1, among which were two SNF2 pro- teins (At3g63950 and At1g05480)
Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth
Visit the PubMed Articlesome overex- pressed genes in hub1-1 were related to meristem development, such as the homeodomain genes KNOTTED1-LIKE2 (KNAT2)
Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth
Visit the PubMed ArticleOf the cytokinesis-related genes, 26 kinesins or kinesin-like genes and genes related to microtubule and myosin were downregulated in hub1-1, among which are the kinesins ... TETRASPORE (NACK2)
Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth
Visit the PubMed Articlesome overex- pressed genes in hub1-1 were related to meristem development, such as ... 12 NO APICAL MERI- STEM genes
Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth
Visit the PubMed ArticleOf the cytokinesis-related genes, 26 kinesins or kinesin-like genes and genes related to microtubule and myosin were downregulated in hub1-1, among which are the ... syntaxin (KNOLLE)
Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth
Visit the PubMed ArticleA total of 13 homeotic genes had an altered expression in hub1-1, among which ... a BRAHMA-like protein (At3g06010) of the Polycomb group of proteins in fruitfly (Dro- sophila melanogaster
Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth
Visit the PubMed ArticleOf the cytokinesis-related genes, 26 kinesins or kinesin-like genes and genes related to microtubule and myosin were downregulated in hub1-1, among which are the ... MAP63-3 (PLEIADE)
Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth
Visit the PubMed ArticleOf the cytokinesis-related genes, 26 kinesins or kinesin-like genes and genes related to microtubule and myosin were downregulated in hub1-1, among which are the kinesins HINKEL (NACK1)
Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth
Visit the PubMed ArticleBiological processes, such as response to stress, proton trans- port, cell redox homeostasis, hexose metabolism, cellular respi- ration, and cofactor catabolism, were significantly overexpressed in hub1-1
Fleury D, Himanen K, Cnops G, Nelissen H, Boccardi TM, Maere S, Beemster GT, Neyt P, Anami S, Robles P, Micol JL, Inzé D, Van Lijsebettens M - The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth
Visit the PubMed ArticleReal time PCR experiments ... in trn2-2 3010 mutant ... did not substantiate a change in WUS transcripts
Chiu WH, Chandler J, Cnops G, Van Lijsebettens M, Werr W - Mutations in the TORNADO2 gene affect cellular decisions in the peripheral zone of the shoot apical meristem of Arabidopsis thaliana
Visit the PubMed ArticleSTM promoter activity ... increased ... in line 3010
Chiu WH, Chandler J, Cnops G, Van Lijsebettens M, Werr W - Mutations in the TORNADO2 gene affect cellular decisions in the peripheral zone of the shoot apical meristem of Arabidopsis thaliana
Visit the PubMed Articletrn2-2 ... mutant meristem ... CLV3 ... expression ... show no major differences in size or position relative to wild type
Chiu WH, Chandler J, Cnops G, Van Lijsebettens M, Werr W - Mutations in the TORNADO2 gene affect cellular decisions in the peripheral zone of the shoot apical meristem of Arabidopsis thaliana
Visit the PubMed Articletrn2-2 ... mutant meristem ... WUS expression ... show no major differences in size or position relative to wild type
Chiu WH, Chandler J, Cnops G, Van Lijsebettens M, Werr W - Mutations in the TORNADO2 gene affect cellular decisions in the peripheral zone of the shoot apical meristem of Arabidopsis thaliana
Visit the PubMed Articlea comparison between trn2-2 3010 seedlings and wild type controls ... shows no significant difference in LFY transcriptional activity in the vegetative SAM
Chiu WH, Chandler J, Cnops G, Van Lijsebettens M, Werr W - Mutations in the TORNADO2 gene affect cellular decisions in the peripheral zone of the shoot apical meristem of Arabidopsis thaliana
Visit the PubMed ArticleTRN2 transcriptional activity was detected in all types of meristems
Chiu WH, Chandler J, Cnops G, Van Lijsebettens M, Werr W - Mutations in the TORNADO2 gene affect cellular decisions in the peripheral zone of the shoot apical meristem of Arabidopsis thaliana
Visit the PubMed ArticleSTM transcript levels in trn2-2 3010 mutant seedlings ... 4-fold increase
Chiu WH, Chandler J, Cnops G, Van Lijsebettens M, Werr W - Mutations in the TORNADO2 gene affect cellular decisions in the peripheral zone of the shoot apical meristem of Arabidopsis thaliana
Visit the PubMed ArticleTRN2 transcripts in leaf primorida are preferentially found in vascular strands
Chiu WH, Chandler J, Cnops G, Van Lijsebettens M, Werr W - Mutations in the TORNADO2 gene affect cellular decisions in the peripheral zone of the shoot apical meristem of Arabidopsis thaliana
Visit the PubMed ArticleTRN2 transcripts in leaf primorida are preferentially found ... at the distal tip of the leaflet
Chiu WH, Chandler J, Cnops G, Van Lijsebettens M, Werr W - Mutations in the TORNADO2 gene affect cellular decisions in the peripheral zone of the shoot apical meristem of Arabidopsis thaliana
Visit the PubMed Articlenor quantification of the STM RNA suggests there are significant quantitative changes in STM expression between mutant and wild type inflorescences
Chiu WH, Chandler J, Cnops G, Van Lijsebettens M, Werr W - Mutations in the TORNADO2 gene affect cellular decisions in the peripheral zone of the shoot apical meristem of Arabidopsis thaliana
Visit the PubMed ArticleTRN2 ... transcripts are ... detectable in lateral organ primordia
Chiu WH, Chandler J, Cnops G, Van Lijsebettens M, Werr W - Mutations in the TORNADO2 gene affect cellular decisions in the peripheral zone of the shoot apical meristem of Arabidopsis thaliana
Visit the PubMed Articleskb1 mutants and wild-type plants did not show a significant difference in transcript levels of ... VRN1
Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis
Visit the PubMed ArticleLoss of SKB1 ... upregulated EMF1
Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis
Visit the PubMed ArticleSKB1 protein levels were ... slightly decreased with age under long-day conditions
Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis
Visit the PubMed ArticleConsistent with the protein expression pattern, SKB1 RNA was more abundant in ... leaf primordia
Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis
Visit the PubMed ArticleConsistent with the protein expression pattern, SKB1 RNA was more abundant in ... young leaves
Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis
Visit the PubMed Articleskb1 mutants and wild-type plants did not show a significant difference in transcript levels of ... SPY
Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis
Visit the PubMed ArticleConsistent with the protein expression pattern, SKB1 ... expressed ... not as much in older leaves
Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis
Visit the PubMed ArticleLoss of SKB1 downregulated ... FT
Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis
Visit the PubMed ArticleConsistent with the protein expression pattern, SKB1 RNA was more abundant in shoot apex
Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis
Visit the PubMed ArticleLoss of SKB1 downregulated ... SOC1
Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis
Visit the PubMed ArticleLoss of SKB1 ... upregulated ... EMF2
Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis
Visit the PubMed ArticleSKB1 protein levels were weakly increased with plant age under short-day condition
Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis
Visit the PubMed ArticleLoss of SKB1 downregulated LFY
Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis
Visit the PubMed Articleskb1 mutants and wild-type plants did not show a significant difference in transcript levels of ... CO
Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis
Visit the PubMed ArticleWestern blotting results showed that SKB1 was highly expressed in flowers, roots and siliques, but less so in stems and mature leaves
Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis
Visit the PubMed ArticleThe expression of the flowering repressor gene FLC, however, was significantly upregulated in skb1-1 mutant plants
Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis
Visit the PubMed Articleskb1 mutants and wild-type plants did not show a significant difference in transcript levels of GI
Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis
Visit the PubMed ArticleLoss of SKB1 ... upregulated ... TFL1
Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis
Visit the PubMed ArticleThe expression of LD, in the autonomous pathway upstream of FLC (He and Amasino, 2005), did not differ between skb1-1 and wild-type plants
Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis
Visit the PubMed Articleskb1 mutants and wild-type plants did not show a significant difference in transcript levels of ... VRN2
Wang X, Zhang Y, Ma Q, Zhang Z, Xue Y, Bao S, Chong K - SKB1-mediated symmetric dimethylation of histone H4R3 controls flowering time in Arabidopsis
Visit the PubMed ArticleP35S:GFP:PARL1 ... nuclear fluores- cence was most strongly localized to a subnuclear re- gion, presumably the nucleolus
Petricka JJ, Nelson TM - Arabidopsis nucleolin affects plant development and patterning
Visit the PubMed ArticleWhen the P35S:GFP:PARL1 con- struct was viewed in the same cells under UV illumi- nation, nuclear fluorescence was observed in cotyledon (Fig. 5K) and leaf (Fig. 5N) cells
Petricka JJ, Nelson TM - Arabidopsis nucleolin affects plant development and patterning
Visit the PubMed ArticleIn young leaf primor- dia PPARL1:GUS expression was initially strong and ubiquitous (Fig. 5, D and F), whereas later expression was restricted to the leaf tip and developing vascular cells
Petricka JJ, Nelson TM - Arabidopsis nucleolin affects plant development and patterning
Visit the PubMed ArticleIn parl1 mutant leaves, DR5:GUS expression was initially correctly localized in the tip at comparable stages, suggesting the incipient midvein pattern was normal
Petricka JJ, Nelson TM - Arabidopsis nucleolin affects plant development and patterning
Visit the PubMed ArticlePARL1 was expressed ... ubiqui- tously throughout the globular stage embryo
Petricka JJ, Nelson TM - Arabidopsis nucleolin affects plant development and patterning
Visit the PubMed ArticleAs auxin response was disrupted in parl1 leaves, we wanted to investigate the role of auxin transport in parl1/DR5:GUS expressing leaves. In wild-type leaves, the inhibition of PAT by naphthylphthalamic acid (NPA) and similar inhibitors leads to restriction of DR5:GUS expression to the leaf margins, possibly re- flecting the accumulation of auxin in these regions (Fig. 6, N and P; Mattsson et al., 2003). Subsequent venation is restricted to the leaf margins. In agreement with this previous report, 1 mM NPA treatment of wild- type plants restricted DR5:GUS expression to the leaf margin (Fig. 6, N and P; Mattsson et al., 2003). In con- trast, this marginal expression was absent in 1 mM NPA-treated leaves of the parl1 mutant (compare Fig. 6, N and P with Fig. 6, O and Q). Thus the expression pattern of DR5:GUS was similar in both NPA-treated and -untreated parl1 leaves
Petricka JJ, Nelson TM - Arabidopsis nucleolin affects plant development and patterning
Visit the PubMed ArticleExpression of the PPARL1:GUS construct was enriched in vascular cells throughout the seedling and was vascular spe- cific in cotyledons
Petricka JJ, Nelson TM - Arabidopsis nucleolin affects plant development and patterning
Visit the PubMed ArticleAthb8:GUS is a re- porter line that marks cells in the provascular stage of development before lignification is observed and is one of the earliest characterized reporters for vein de- velopment (Baima et al., 1995; Kang and Dengler, 2002; Scarpella et al., 2004, 2006). In wild-type plants, ex- pression of this reporter was initially visible in both wild-type and the parl1/Athb8:GUS line leaf primor- dia as a single file of expression that extended from the petiole to the tip of leaf blade, marking cells that later differentiate into the midvein (Fig. 6A, inset). Next, as previously reported, two pairs of expression traces connecting to the central line in high-arching loops were observed in the Athb8:GUS line that mark cells destined to become secondary vein loops (Fig. 6A). While parl1 mutants resembled the wild type for early midvein expression, in subsequent stages multiple traces of Athb8:GUS expression extended from the petiole into the leaf primordia, and often ended freely (Fig. 6B). This expression pattern change continued with subsequent vein initiation in parl1 (Fig. 6D). In wild-type plants, the expression of Athb8:GUS marked the closed, interconnected vein pattern observed in normal leaves at later stages of development and expansion (Fig. 6C). However, parl1 at this same stage displayed an Athb8:GUS expression pattern that was unconnected and consisted of freely ending parallel veins
Petricka JJ, Nelson TM - Arabidopsis nucleolin affects plant development and patterning
Visit the PubMed ArticleThis apparently normal tip expression of DR5 persisted into the next stage, when subsequent veins were initiated. How- ever, parl1 mutants failed to show DR5:GUS expres- sion in apical loops, as is observed in wild type (Fig. 6J) and subsequently failed to differentiate veins in apical loops. At the next stage and subsequent stages, the persistent tip expression typical of DR5:GUS in wild- type leaves was drastically mislocalized in parl1 leaves of comparable stages (compare Fig. 6, K–M with Fig. 6, G and H). In the mutant, expression ceased at the tip, and ectopic DR5:GUS expression appeared in one or two foci offset from the tip. This mislocalization was observed at 9 d after germination (DAG) in 66% (n 5 502) and at 10 DAG in 86% (n 5 349) of parl1/DR5:GUS leaves and never (7 DAG, n 5 178; 8 DAG, n 5 274) in the wild type
Petricka JJ, Nelson TM - Arabidopsis nucleolin affects plant development and patterning
Visit the PubMed ArticleFurther analysis of the PPARL1: GUS construct in other tissues revealed expression of the PARL1 gene in regions associated with cell divi- sion (root and leaf tips) as well as in vascular cells of roots and flowers
Petricka JJ, Nelson TM - Arabidopsis nucleolin affects plant development and patterning
Visit the PubMed ArticlePARL1 ... ubiquitous GUS expression was also observed later in ... globular ... stages of embry- onic development
Petricka JJ, Nelson TM - Arabidopsis nucleolin affects plant development and patterning
Visit the PubMed ArticlePARL1 ... ubiquitous GUS expression was also observed later in ... the ... heart stages of embry- onic development
Petricka JJ, Nelson TM - Arabidopsis nucleolin affects plant development and patterning
Visit the PubMed ArticleIn young leaf primor- dia PPARL1:GUS expression was initially strong and ubiquitous
Petricka JJ, Nelson TM - Arabidopsis nucleolin affects plant development and patterning
Visit the PubMed ArticleIn young leaf primor- dia PPARL1:GUS expression was initially strong and ubiquitous (Fig. 5, D and F), whereas later expression was restricted to the leaf tip and developing vascular cells
Petricka JJ, Nelson TM - Arabidopsis nucleolin affects plant development and patterning
Visit the PubMed Articlein the vfb1-1 vfb2-1 vfb3-1 vfb4-1 (RNAi) mutants ... The group of repressed auxin signaling genes includes the AUX/IAA repressors ... IAA29
Schwager KM, Calderon-Villalobos LI, Dohmann EM, Willige BC, Knierer S, Nill C, Schwechheimer C - Characterization of the VIER F-BOX PROTEINE genes from Arabidopsis reveals their importance for plant growth and development
Visit the PubMed Articlethe promoter:GUS constructs for VFB1 ... showed strong GUS staining in many plant tissues, including the vascular system, leaves, flowers, and roots
Schwager KM, Calderon-Villalobos LI, Dohmann EM, Willige BC, Knierer S, Nill C, Schwechheimer C - Characterization of the VIER F-BOX PROTEINE genes from Arabidopsis reveals their importance for plant growth and development
Visit the PubMed Articlein the vfb1-1 vfb2-1 vfb3-1 vfb4-1 (RNAi) mutants ... The group of repressed auxin signaling genes includes the AUX/IAA repressors ... IAA3 (SHORT HYPOCOTYL2 [SHY2])
Schwager KM, Calderon-Villalobos LI, Dohmann EM, Willige BC, Knierer S, Nill C, Schwechheimer C - Characterization of the VIER F-BOX PROTEINE genes from Arabidopsis reveals their importance for plant growth and development
Visit the PubMed Articlein the vfb1-1 vfb2-1 vfb3-1 vfb4-1 (RNAi) mutants ... The group of repressed auxin signaling genes includes the AUX/IAA repressors ... IAA6
Schwager KM, Calderon-Villalobos LI, Dohmann EM, Willige BC, Knierer S, Nill C, Schwechheimer C - Characterization of the VIER F-BOX PROTEINE genes from Arabidopsis reveals their importance for plant growth and development
Visit the PubMed Articlein the vfb1-1 vfb2-1 vfb3-1 vfb4-1 (RNAi) mutants ... The group of repressed auxin signaling genes includes the AUX/IAA repressors ... IAA19 (MASSUGU2 [MSG2])
Schwager KM, Calderon-Villalobos LI, Dohmann EM, Willige BC, Knierer S, Nill C, Schwechheimer C - Characterization of the VIER F-BOX PROTEINE genes from Arabidopsis reveals their importance for plant growth and development
Visit the PubMed Article35S:VFB2:GFP ... and 35S:YFP:VFB2 localize to the cytoplasm when expressed in a transient manner in Arabidopsis protoplasts ... Similarly, a VFB2 promoter (2-kb fragment)–driven VFB2:GFP fusion protein also localized predominantly to the cytoplasm (data not shown
Schwager KM, Calderon-Villalobos LI, Dohmann EM, Willige BC, Knierer S, Nill C, Schwechheimer C - Characterization of the VIER F-BOX PROTEINE genes from Arabidopsis reveals their importance for plant growth and development
Visit the PubMed ArticleVFB2 ... also seem to be expressed in the cotyledons of bent cotyledon stage embryos but not at earlier stages of embryo development
Schwager KM, Calderon-Villalobos LI, Dohmann EM, Willige BC, Knierer S, Nill C, Schwechheimer C - Characterization of the VIER F-BOX PROTEINE genes from Arabidopsis reveals their importance for plant growth and development
Visit the PubMed Articlethe promoter:GUS constructs for ... VFB2 ... showed strong GUS staining in many plant tissues, including the vascular system, leaves, flowers, and roots
Schwager KM, Calderon-Villalobos LI, Dohmann EM, Willige BC, Knierer S, Nill C, Schwechheimer C - Characterization of the VIER F-BOX PROTEINE genes from Arabidopsis reveals their importance for plant growth and development
Visit the PubMed Article35S:VFB4:GFP ... localize to the cytoplasm when expressed in a transient manner in Arabidopsis protoplasts
Schwager KM, Calderon-Villalobos LI, Dohmann EM, Willige BC, Knierer S, Nill C, Schwechheimer C - Characterization of the VIER F-BOX PROTEINE genes from Arabidopsis reveals their importance for plant growth and development
Visit the PubMed ArticleVFB4 also seem to be expressed in the cotyledons of bent cotyledon stage embryos but not at earlier stages of embryo development
Schwager KM, Calderon-Villalobos LI, Dohmann EM, Willige BC, Knierer S, Nill C, Schwechheimer C - Characterization of the VIER F-BOX PROTEINE genes from Arabidopsis reveals their importance for plant growth and development
Visit the PubMed Articlein the vfb1-1 vfb2-1 vfb3-1 vfb4-1 (RNAi) mutants ... In agreement with previous reports, we detected DR5:GUS staining in the leaf margins, at sites of lateral root initiation, and in the root tips of wild-type seedlings. Interestingly, the staining at these sites was reduced in the vfb mutant background, suggesting—in agreement with our microaray data—that auxin-responsive gene expression is impaired in the vfb mutant background
Schwager KM, Calderon-Villalobos LI, Dohmann EM, Willige BC, Knierer S, Nill C, Schwechheimer C - Characterization of the VIER F-BOX PROTEINE genes from Arabidopsis reveals their importance for plant growth and development
Visit the PubMed Articlein the vfb1-1 vfb2-1 vfb3-1 vfb4-1 (RNAi) mutants ... The group of repressed auxin signaling genes includes the AUX/IAA repressors ... IAA5
Schwager KM, Calderon-Villalobos LI, Dohmann EM, Willige BC, Knierer S, Nill C, Schwechheimer C - Characterization of the VIER F-BOX PROTEINE genes from Arabidopsis reveals their importance for plant growth and development
Visit the PubMed Articlein the vfb1-1 vfb2-1 vfb3-1 vfb4-1 (RNAi) mutants ... The group of repressed auxin signaling genes includes the AUX/IAA repressors ... IAA2
Schwager KM, Calderon-Villalobos LI, Dohmann EM, Willige BC, Knierer S, Nill C, Schwechheimer C - Characterization of the VIER F-BOX PROTEINE genes from Arabidopsis reveals their importance for plant growth and development
Visit the PubMed Articlein the vfb1-1 vfb2-1 vfb3-1 vfb4-1 (RNAi) mutants ... The group of repressed auxin signaling genes includes the AUX/IAA repressors IAA1 (AXR5)
Schwager KM, Calderon-Villalobos LI, Dohmann EM, Willige BC, Knierer S, Nill C, Schwechheimer C - Characterization of the VIER F-BOX PROTEINE genes from Arabidopsis reveals their importance for plant growth and development
Visit the PubMed Articlethe promoter:GUS constructs for ... VFB4 showed strong GUS staining in many plant tissues, including the vascular system, leaves, flowers, and roots
Schwager KM, Calderon-Villalobos LI, Dohmann EM, Willige BC, Knierer S, Nill C, Schwechheimer C - Characterization of the VIER F-BOX PROTEINE genes from Arabidopsis reveals their importance for plant growth and development
Visit the PubMed ArticleWe found that, in 4-day-old seedlings, AtOFP1prom:GUS was expressed mainly in the roots and lower part of hypocotyls, but not in root hairs, lateral root primordium or shoot (Figure 5a). AtOFP1prom:GUS was still expressed in the roots of 10-day-old seedlings including root hair (Figure 5b), but unlike the 4-day-old seedlings, it was also expressed in shoots, vasculatures and trichomes
Wang S, Chang Y, Guo J, Chen JG - Arabidopsis Ovate Family Protein 1 is a transcriptional repressor that suppresses cell elongation
Visit the PubMed ArticleBy using these transgenic plants, we found that AtOFP1 is predominantly localized in the nucleus ... In a transient expression system using protoplasts derived from Arabidopsis rosette leaves, we also observed a nuclear-localized AtOFP1–GFP fusion protein when the 35S promoter was used to drive AtOFP1–GFP expression (Figure 5h), which is largely consistent with the results reported by Hackbusch et al. (2005), except that we did not observe a significant association of AtOFP1–GFP with the cytoskeleton
Wang S, Chang Y, Guo J, Chen JG - Arabidopsis Ovate Family Protein 1 is a transcriptional repressor that suppresses cell elongation
Visit the PubMed ArticleWe found that, in 4-day-old seedlings, AtOFP1prom:GUS was expressed mainly in the roots and lower part of hypocotyls, but not in root hairs, lateral root primordium or shoot (Figure 5a). AtOFP1prom:GUS was still expressed in the roots of 10-day-old seedlings including root hair (Figure 5b), but unlike the 4-day-old seedlings, it was also expressed in shoots, vasculatures and trichomes
Wang S, Chang Y, Guo J, Chen JG - Arabidopsis Ovate Family Protein 1 is a transcriptional repressor that suppresses cell elongation
Visit the PubMed Articlewe found AtOFP1 transcript in the roots of young seedlings and both roots and shoots of relatively old seedlings
Wang S, Chang Y, Guo J, Chen JG - Arabidopsis Ovate Family Protein 1 is a transcriptional repressor that suppresses cell elongation
Visit the PubMed ArticleWe found that, in 4-day-old seedlings, AtOFP1prom:GUS was expressed mainly in the roots and lower part of hypocotyls, but not in root hairs, lateral root primordium or shoot (Figure 5a). AtOFP1prom:GUS was still expressed in the roots of 10-day-old seedlings including root hair (Figure 5b), but unlike the 4-day-old seedlings, it was also expressed in shoots, vasculatures and trichomes
Wang S, Chang Y, Guo J, Chen JG - Arabidopsis Ovate Family Protein 1 is a transcriptional repressor that suppresses cell elongation
Visit the PubMed ArticleTransgenic plants were generated carrying the GUS reporter gene driven by a 2.2 kb BEN1 promoter to examine tissue-specific expression patterns of BEN1 ... In mature rosette leaves, GUS expression was seen mainly in ... hydathodes
Yuan T, Fujioka S, Takatsuto S, Matsumoto S, Gou X, He K, Russell SD, Li J - BEN1, a gene encoding a dihydroflavonol 4-reductase (DFR)-like protein, regulates the levels of brassinosteroids in Arabidopsis thaliana
Visit the PubMed Articlethe expression of BEN1 is indeed significantly downregulated in darkness
Yuan T, Fujioka S, Takatsuto S, Matsumoto S, Gou X, He K, Russell SD, Li J - BEN1, a gene encoding a dihydroflavonol 4-reductase (DFR)-like protein, regulates the levels of brassinosteroids in Arabidopsis thaliana
Visit the PubMed ArticleBAS1 was significantly downregulated ... in ben1-1D mutant plants, compared to wild-type plants
Yuan T, Fujioka S, Takatsuto S, Matsumoto S, Gou X, He K, Russell SD, Li J - BEN1, a gene encoding a dihydroflavonol 4-reductase (DFR)-like protein, regulates the levels of brassinosteroids in Arabidopsis thaliana
Visit the PubMed ArticleTransgenic plants were generated carrying the GUS reporter gene driven by a 2.2 kb BEN1 promoter to examine tissue-specific expression patterns of BEN1 ... In mature rosette leaves, GUS expression was seen mainly in vascular tissues
Yuan T, Fujioka S, Takatsuto S, Matsumoto S, Gou X, He K, Russell SD, Li J - BEN1, a gene encoding a dihydroflavonol 4-reductase (DFR)-like protein, regulates the levels of brassinosteroids in Arabidopsis thaliana
Visit the PubMed ArticleRT-PCR results also showed that BEN1 transcripts can be detected in all major organs tested, with the strongest signals detected in roots and siliques
Yuan T, Fujioka S, Takatsuto S, Matsumoto S, Gou X, He K, Russell SD, Li J - BEN1, a gene encoding a dihydroflavonol 4-reductase (DFR)-like protein, regulates the levels of brassinosteroids in Arabidopsis thaliana
Visit the PubMed ArticleCPD is downregulated when BR levels are increased
Yuan T, Fujioka S, Takatsuto S, Matsumoto S, Gou X, He K, Russell SD, Li J - BEN1, a gene encoding a dihydroflavonol 4-reductase (DFR)-like protein, regulates the levels of brassinosteroids in Arabidopsis thaliana
Visit the PubMed ArticleThe subcellular localization revealed using bri1-5 35S-BEN1–GFP should thus represent the real in vivo site of the BEN1 protein. Confocal analyses indicated that BEN1 is a cytoplasmic protein
Yuan T, Fujioka S, Takatsuto S, Matsumoto S, Gou X, He K, Russell SD, Li J - BEN1, a gene encoding a dihydroflavonol 4-reductase (DFR)-like protein, regulates the levels of brassinosteroids in Arabidopsis thaliana
Visit the PubMed ArticleRT-PCR analyses also indicated that the expression of BEN1 in wild-type plants cannot be significantly regulated by exogenously applied BL
Yuan T, Fujioka S, Takatsuto S, Matsumoto S, Gou X, He K, Russell SD, Li J - BEN1, a gene encoding a dihydroflavonol 4-reductase (DFR)-like protein, regulates the levels of brassinosteroids in Arabidopsis thaliana
Visit the PubMed ArticleBAS1 is upregulated in response to exogenously elevated BR levels
Yuan T, Fujioka S, Takatsuto S, Matsumoto S, Gou X, He K, Russell SD, Li J - BEN1, a gene encoding a dihydroflavonol 4-reductase (DFR)-like protein, regulates the levels of brassinosteroids in Arabidopsis thaliana
Visit the PubMed ArticleCPD was significantly upregulated in ben1-1D mutant plants, compared to wild-type plants
Yuan T, Fujioka S, Takatsuto S, Matsumoto S, Gou X, He K, Russell SD, Li J - BEN1, a gene encoding a dihydroflavonol 4-reductase (DFR)-like protein, regulates the levels of brassinosteroids in Arabidopsis thaliana
Visit the PubMed Articlelevel of transcripts of the ... KAN2 ... increased in the ... as1-1
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed Articlethe level of ARF4 did not clearly increase in as2-1
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed Articlein as1-1 ... No significant differences in levels of transcripts were detected for ... YAB3
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed Articlein as2-1 ... No significant differences in levels of transcripts were detected for ... YAB2
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed Articlein as2-1 ... No significant differences in levels of transcripts were detected for ... YAB3
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed ArticleAS1 transcripts ... As primordia grew (P4–P6), we detected strong signals in the central region between the adaxial and abaxial domains (Figure 1l,m). The
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed Articlelevel of transcripts of the ETT/ARF3 ... increased in the as2-1
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed ArticleLevels of ARF2 transcripts were not affected by ... as2
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed Articlelevel of transcripts of the ... KAN2 ... decreased in the as2-1/pAS1::AS2#4 plants
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed ArticleThe level of FIL transcripts ... did not clearly increase in as1-1
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed Articlelevel of transcripts of the ... class 1 KNOX genes, increased in the as2-1
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed Articlein as1-1 ... No significant differences in levels of transcripts were detected for ... KAN3
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed Articlelevel of transcripts of the ... class 1 KNOX genes ... decreased in the as2-1/pAS1::AS2#4 plants
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed ArticleLevels of ARF2 transcripts were not affected by ... as1
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed Articlein as2-1 ... No significant differences in levels of transcripts were detected for ... PHV
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed Articlelevel of transcripts of the ... KAN2 ... increased in the as2-1
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed ArticleWe obtained five independent transgenic lines that harbored pAS2::GUS and all yielded essentially the same pattern of expression of GUS activity ... When leaves had developed (21-day-old plants), we detected GUS activity over the entire adaxial surface of leaves
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed Articlelevel of transcripts of the ETT/ARF3 ... increased in the ... as1-1
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed ArticleThe level of FIL transcripts ... decreased in the as2-1/pAS1::AS2#4 plants
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed ArticleWe obtained five independent transgenic lines that harbored pAS2::GUS and all yielded essentially the same pattern of expression of GUS activity ... we detected GUS activity on the adaxial sides of cotyledons
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed ArticleWe obtained eight independent pAS1::GUS transgenic lines and detected GUS activity mainly in inner tissues, which included the vascu- lature of leaves, hypocotyls and roots
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed ArticleLevels of transcripts of the BP/KNAT1 ... decreased in as2-1/pAS1::AS2#4 plants
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed ArticleThe level of FIL transcripts increased in as2-1
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed ArticleWe obtained five independent transgenic lines that harbored pAS2::GUS and all yielded essentially the same pattern of expression of GUS activity ... The activity in cotyledons and leaves disappeared as the leaves grew further
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed Articlelevel of transcripts of the ... YAB5 ... increased in the ... as1-1
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed ArticleIn P0– P3, we detected weak signals due to AS1 in entire primordia
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed Articlein as2-1 ... No significant differences in levels of transcripts were detected for KAN1
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed Articlelevel of transcripts of the ... YAB5 ... increased in the as2-1
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed Articlelevel of transcripts of the ... YAB5 ... decreased in the as2-1/pAS1::AS2#4 plants
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed ArticleLevels of transcripts of the ... KNAT6 ... decreased in as2-1/pAS1::AS2#4 plants
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed ArticleAS1 transcripts ... In more developed primordia (after P8), we detected signals in both the vasculature and lateral regions
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed Articlein as1-1 ... No significant differences in levels of transcripts were detected for KAN1
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed ArticleAS1 transcripts were clearly evident in the inner tissues of leaves (Figure 1l,m), as reported by Byrne et al. (2000
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed Articlethe level of ARF4 ... decreased in the as2-1/pAS1::AS2#4 plants
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed ArticleThe level of ARF4 transcripts increased in as1-1
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed ArticleLevels of transcripts of the ... KNAT6 ... increased in the ... as1-1
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed Articlelevel of transcripts of the ... class 1 KNOX genes, increased in the ... as1-1
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed Articlein as1-1 ... No significant differences in levels of transcripts were detected for ... PHV
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed ArticleLevels of transcripts of the ... KNAT6 ... increased in the as2-1
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed Articlein as1-1 ... No significant differences in levels of transcripts were detected for ... KAN4
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed Articlein as2-1 ... No significant differences in levels of transcripts were detected for ... PHB
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed Articlein as2-1 ... No significant differences in levels of transcripts were detected for ... KAN4
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed ArticleThe level of the KNAT2 gene transcript was not clearly altered in shoot apices of as2-1
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed ArticleLevels of transcripts of the BP/KNAT1 ... increased in the ... as1-1
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed ArticleWe performed in situ hybridization in order to detect AS2 transcripts in plantlets ... When primordia had developed, we detected stronger signals in the adaxial region, including the epidermis of leaf primordia. However, weak signals were also evident in the inner region (P3–P9; Figure 1a–f
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed ArticleWe obtained eight independent pAS1::GUS transgenic lines ... When leaves had developed (24-day-old plants), GUS signals in the vasculature of cotyledons and leaves became weaker, as previously shown by RT-PCR (Figure 1p) (Chua et al., 2005)
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed ArticleWe performed in situ hybridization in order to detect AS2 transcripts in plantlets. In transverse and longitudinal sections of 12-day-old plants, we detected relatively weak signals due to AS2 transcripts in the region of the predicted leaf primordium (P0) and in early detectable primordia (P1 and P2) (Figure 1a–f,r
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed Articlein as1-1 ... No significant differences in levels of transcripts were detected for ... YAB2
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed ArticleWe obtained five independent transgenic lines that harbored pAS2::GUS and all yielded essentially the same pattern of expression of GUS activity ... we detected GUS activity on the adaxial sides of ... leaves
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed ArticleLevels of transcripts of the BP/KNAT1 ... increased in the as2-1
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed Articlein as2-1 ... No significant differences in levels of transcripts were detected for ... KAN3
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed Articlein as1-1 ... No significant differences in levels of transcripts were detected for ... PHB
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed Articlein as2-1 ... No significant differences in levels of transcripts were detected for ... REV
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed Articlelevel of transcripts of the ETT/ARF3 ... decreased in the as2-1/pAS1::AS2#4 plants
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed Articlein as1-1 ... No significant differences in levels of transcripts were detected for ... REV
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed ArticleAS1 transcripts ... In more developed primordia (after P8), we detected signals in ... the vasculature
Iwakawa H, Iwasaki M, Kojima S, Ueno Y, Soma T, Tanaka H, Semiarti E, Machida Y, Machida C - Expression of the ASYMMETRIC LEAVES2 gene in the adaxial domain of Arabidopsis leaves represses cell proliferation in this domain and is critical for the development of properly expanded leaves
Visit the PubMed ArticlepANT::GUS ... GUS activity was seen throughout very young leaves at Day 0
Kang J, Mizukami Y, Wang H, Fowke L, Dengler NG - Modification of cell proliferation patterns alters leaf vein architecture in Arabidopsis thaliana
Visit the PubMed ArticlepANT::GUS ... GUS activity ... declined basipetally in the leaf blades at Day 4
Kang J, Mizukami Y, Wang H, Fowke L, Dengler NG - Modification of cell proliferation patterns alters leaf vein architecture in Arabidopsis thaliana
Visit the PubMed ArticleCross sections of the leaf revealed that pANT::GUS expression was seen in procambial cells prior to vascular differentiation (Fig. 2b), remained in procambial cells during vein differentiation (Fig. 2b asterisks), and declined as procambial cells differentiated into xylem and phloem
Kang J, Mizukami Y, Wang H, Fowke L, Dengler NG - Modification of cell proliferation patterns alters leaf vein architecture in Arabidopsis thaliana
Visit the PubMed ArticlepANT::GUS ... GUS activity ... remained high in developing veins through to Day 8
Kang J, Mizukami Y, Wang H, Fowke L, Dengler NG - Modification of cell proliferation patterns alters leaf vein architecture in Arabidopsis thaliana
Visit the PubMed Articlethe number of cells accumulating CYCB1;1::GUS in the apical half of leaves was similar to that in wild-type leaves at Day 0, there appeared to be fewer GUS expressing cells in ant-1 leaves by Day 4 compared to wild-type leaves at the same stage
Kang J, Mizukami Y, Wang H, Fowke L, Dengler NG - Modification of cell proliferation patterns alters leaf vein architecture in Arabidopsis thaliana
Visit the PubMed ArticlepANT::GUS expression in procambial cells disappeared after leaf maturation (data not shown)
Kang J, Mizukami Y, Wang H, Fowke L, Dengler NG - Modification of cell proliferation patterns alters leaf vein architecture in Arabidopsis thaliana
Visit the PubMed ArticleIn 35S::ICK1 leaves, CYCB1;1::GUS accumulation was sharply reduced in comparison to wild-type at all stages, reflecting a repression of cell proliferation throughout leaf development
Kang J, Mizukami Y, Wang H, Fowke L, Dengler NG - Modification of cell proliferation patterns alters leaf vein architecture in Arabidopsis thaliana
Visit the PubMed ArticlemRNA level of FLOWERING LOCUS K (FLK), a component of autonomous pathway, was increased by 2-fold ... in atprmt5 mutants as compared to wild-type
Pei Y, Niu L, Lu F, Liu C, Zhai J, Kong X, Cao X - Mutations in the Type II protein arginine methyltransferase AtPRMT5 result in pleiotropic developmental defects in Arabidopsis
Visit the PubMed ArticleExpression of FLC was dramatically increased due to mutations in AtPRMT5
Pei Y, Niu L, Lu F, Liu C, Zhai J, Kong X, Cao X - Mutations in the Type II protein arginine methyltransferase AtPRMT5 result in pleiotropic developmental defects in Arabidopsis
Visit the PubMed Articledue to mutations in AtPRMT5 ... expression of SUPPRESSOR OF OVEREXPRESSION OF CO1 (SOC1)/AGAMOUS-LIKE20, a floral integrator downstream of FLC, was greatly reduced
Pei Y, Niu L, Lu F, Liu C, Zhai J, Kong X, Cao X - Mutations in the Type II protein arginine methyltransferase AtPRMT5 result in pleiotropic developmental defects in Arabidopsis
Visit the PubMed ArticlemRNA level of ... ARP6 (ACTIN-RELATED PROTEIN6), an activator of FLC, was decreased by 2-fold in atprmt5 mutants as compared to wild-type Col
Pei Y, Niu L, Lu F, Liu C, Zhai J, Kong X, Cao X - Mutations in the Type II protein arginine methyltransferase AtPRMT5 result in pleiotropic developmental defects in Arabidopsis
Visit the PubMed Articleno FIL expression was detected in ... as1 ... differentiated leaves
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed Articlewe could not detect a significant change in the expression levels of FIL ... in ... bop1-4 bop2-11 plants
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleFIL transcripts were likewise detected on the adaxial side of bop1 bop2 as2 ... differentiated leaves but with the expression in the more central region of the leaf
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleThe expression levels of ... KNAT2 ... were significantly increased in bop1-4 bop2-11 seedlings
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleThe domain of PHV expression is unaltered in ... as1 seedlings
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleLBD36/ASL1 transcript levels were elevated in 35S:BOP1 ... plants
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed Articlein ... bop1 bop2 as1 ... plants, FIL expression was detected on the abaxial side of initiating and young leaf primordia
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleLBD36/ASL1 transcript levels were elevated in ... 35S:BOP2 plants
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed Articlein bop1 bop2 ... plants, FIL expression was detected on the abaxial side of initiating and young leaf primordia
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed Articlewe could not detect a significant change in the expression levels of FIL ... in ... bop2-11 ... plants
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleAS1 transcript levels were slightly elevated in ... bop1-4 bop2-11 plants compared with the wild type
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticlePHV expression is not detected in the differentiated leaves of ... as1
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed Articlewe found that PHB transcription was slightly upregulated ... in the developing young leaves of 22-d-old 35S:BOP1 leaves compared with wild-type leaves
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleIn as2 ... FIL transcripts are detected in the correct abaxial domain ... of initiating and young leaf primordia
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleThe expression levels of BP ... were significantly increased in bop1-4 bop2-11 seedlings
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed Articlein ... bop1 bop2 kan1 kan2 plants, FIL expression was detected on the abaxial side of initiating and young leaf primordia
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleKAN1 transcription was slightly reduced in the developing young leaves of 22-d-old 35S:BOP1 leaves compared with wild-type leaves
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed Articlemore mature ... bop1 bop2 as1 ... leaves exhibited ectopic FIL transcription in the adaxial domain
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleAS1 expression levels were unchanged in ... 35S:BOP2 plants
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed Articlewe could not detect a significant change in the expression levels of ... KAN1 ... in bop1-4
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleAS2 transcript levels were also reduced in bop1-1 ... plants
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleExpression levels of ... AS2 ... were highly upregulated in 35S:BOP1 ... plants
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleDeveloping young leaves of ... bop1 bop2 as1 ... exhibited PHV expression in the normal adaxial domain
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleAS1 transcript levels were slightly elevated in bop1-1 ... plants compared with the wild type
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed Articleno FIL expression was detected in as2 ... differentiated leaves
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleLBD36/ASL1 transcript levels ... were reduced in bop1-1 ... plants
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed Articlewe could not detect a significant change in the expression levels of FIL ... in bop1-4
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed Articlelob:ET22 GUS ... We also detected ectopic GUS activity broadly across the blade region of 35S:BOP1 leaves ... a pattern that was not observed in lob leaves
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed Articlewe could not detect a significant change in the expression levels of ... KAN1 ... in ... bop2-11 ... plants
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticlePHV expression ... can be observed on the adaxial side of the differentiated leaves of ... bop1 bop2 as1 ... Furthermore, PHV was expressed more centrally in ... bop1 bop2 as1 ... leaves than in bop1 bop2 leaves
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleThe differentiated leaves of bop1 bop2 plants exhibited FIL expression on the adaxial side of the primordial ectopic organ outgrowths (Figure 8P, arrows), while FIL transcripts were not detected in differentiated leaves of Col plants
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleExpression levels of ... LOB were highly upregulated in 35S:BOP1 ... plants
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleIn kan1 kan2 seedlings, PHV expression is detected throughout the leaf primordia, but a stronger signal is observed on the adaxial side than on the abaxial side (Figure 9D), suggesting that these organs still maintain some residual asymmetry
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleIn bop1-4 bop2-11 plants containing lob:ET22, GUS expression was observed in the shoot apex but more weakly than in lob plants
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleExpression levels of ... LOB were highly upregulated in ... 35S:BOP2 plants
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed Articlein bop1 bop2 leaves, KAN1 expression is detected in the adaxial region in the area in which the ectopic organ outgrowths develop
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed Articlein ... bop1 bop2 as2 ... plants, FIL expression was detected on the abaxial side of initiating and young leaf primordia
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleFIL transcripts were likewise detected on the adaxial side of ... bop1 bop2 kan1 kan2 differentiated leaves but with the expression in the more central region of the leaf
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticlePHB is one of the class III HD-ZIP genes that specify adaxial cell fate, and its steady state transcript levels were increased in 8-d-old 35S:BOP1 seedlings compared with wild-type seedlings
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleLOB expression was decreased in ... bop1-4 bop2-11 plants
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed Articleno FIL expression was detected in ... kan1 kan2 differentiated leaves
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleExpression levels of ... AS2 ... were highly upregulated in ... 35S:BOP2 plants
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleDeveloping young leaves of ... bop1 bop2 as2 ... exhibited PHV expression in the normal adaxial domain
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticlePHB ... we could not detect a significant change in the expression levels ... in ... bop1-4 bop2-11 plants
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleThe domain of PHV expression is unaltered in as2 ... seedlings
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed Articlein 8-d-old 35S:BOP1 seedlings ... we could not detect a significant change in the expression levels of FIL
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleAS2 transcript levels were also reduced in ... bop1-4 bop2-11 plants
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed Articlemore mature ... bop1 bop2 kan1 kan2 leaves exhibited ectopic FIL transcription in the adaxial domain
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleBP mRNA levels were reduced in the shoots of 25-d-old 35S:BOP1 ... plants
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed Articleectopic expression of BOP1 resulted in ... expanded lob:ET22 GUS expression in the shoot apex
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed Articlemore mature ... bop1 bop2 as2 ... leaves exhibited ectopic FIL transcription in the adaxial domain
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleDeveloping young leaves of ... bop1 bop2 kan1 kan2 seedlings exhibited PHV expression in the normal adaxial domain (Figures 9I to 9L). However, in bop1 bop2 kan1 kan2 leaves, PHV expression is restricted to a smaller area of the adaxial domain than in kan1 kan2 leaves (Figures 9D and 9L, arrow). This result indicates that, whereas the young leaves of kan1 kan2 plants have adaxialized organ identity, the young leaves of quadruple mutant plants acquire abaxialized organ identity, which is consistent with histological data
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticlePHV expression is not detected in the differentiated leaves of ... kan1 kan2 plants
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleIn lob plants, the lob:ET22 enhancer trap drove GUS reporter gene expression at the shoot apex
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed Articleectopic expression of BOP1 resulted in increased ... lob:ET22 GUS expression in the shoot apex
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticlePHV expression is not detected in the differentiated leaves of ... as2
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticlePHB ... we could not detect a significant change in the expression levels ... in ... bop2-11
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticlePHV expression ... can be observed on the adaxial side of the differentiated leaves of ... bop1 bop2 kan1 kan2 plants ... Furthermore, PHV was expressed more centrally in ... bop1 bop2 kan1 kan2 leaves than in bop1 bop2 leaves
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed Articlewe could not detect a significant change in the expression levels of ... KAN1 ... in ... bop1-4 bop2-11 plants
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleLOB expression was decreased in bop1-1 ... plants
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleBP mRNA levels were reduced in the shoots of 25-d-old ... 35S:BOP2 plants
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleFIL ... transcription was slightly reduced in the developing young leaves of 22-d-old 35S:BOP1 leaves compared with wild-type leaves
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticlePHB ... we could not detect a significant change in the expression levels ... in bop1-4 ... plants
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleIn bop1-1 plants containing lob:ET22, we could not detect GUS gene activity in the shoot apex
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticlePHV expression ... can be observed on the adaxial side of the differentiated leaves of ... bop1 bop2 as2 ... Furthermore, PHV was expressed more centrally in bop1 bop2 as2 ... leaves than in bop1 bop2 leaves
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleIn ... kan1 kan2 ... FIL transcripts are detected in the correct abaxial domain ... of initiating and young leaf primordia
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleFIL transcripts were likewise detected on the adaxial side of ... bop1 bop2 as1 ... differentiated leaves but with the expression in the more central region of the leaf
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleIn ... as1 ... FIL transcripts are detected in the correct abaxial domain ... of initiating and young leaf primordia
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleAS1 expression levels were unchanged in ... 35S:BOP1 ... plants
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed Articlein 8-d-old 35S:BOP1 seedlings ... we could not detect a significant change in the expression levels of ... KAN1
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticlePHV expression ... can be observed on the adaxial side of the differentiated leaves of bop1 bop2
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleDeveloping young leaves of bop1 bop2 ... exhibited PHV expression in the normal adaxial domain
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleIn lob plants, the lob:ET22 enhancer trap drove GUS reporter gene expression at the shoot apex, in the hypocotyl, at the base of all lateral organs, and in the roots
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleThe expression levels of ... KNAT6 ... were significantly increased in bop1-4 bop2-11 seedlings
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed Articlemore mature bop1 bop2 ... leaves exhibited ectopic FIL transcription in the adaxial domain
Ha CM, Jun JH, Nam HG, Fletcher JC - BLADE-ON-PETIOLE 1 and 2 control Arabidopsis lateral organ fate through regulation of LOB domain and adaxial-abaxial polarity genes
Visit the PubMed ArticleThe abundance of AXL mRNA was extremely low compared with AXR1 mRNA (data not shown). Although AXL RNA was detected in all the tissue types examined, root tissues showed the highest RNA abundance. However, at least 50 PCR cycles were required to obtain a band detectable by standard agarose gel electrophoresis. The relatively low level of expression of AXL in all tissues is also confirmed by microarray analysis
Dharmasiri N, Dharmasiri S, Weijers D, Karunarathna N, Jurgens G, Estelle M - AXL and AXR1 have redundant functions in RUB conjugation and growth and development in Arabidopsis
Visit the PubMed Articlethe level of the EBP1 mRNA was found to be around twofold ... lower in the ... AtTOR-silenced lines
Deprost D, Yao L, Sormani R, Moreau M, Leterreux G, Nicolaï M, Bedu M, Robaglia C, Meyer C - The Arabidopsis TOR kinase links plant growth, yield, stress resistance and mRNA translation
Visit the PubMed ArticleA higher expression of the AtEBP1 gene was indeed detected in the AtTOR-overexpressing line S7817 when compared with Col0 after hybridization of CATMA arrays (urgv.evry.inra.fr/cgi-bin/projects/CATdb; D. Deprost & C. Meyer, unpublished data). These preliminary results were confirmed by quantitative real-time reverse transcriptase–PCR and the level of the EBP1 mRNA was found to be around twofold higher ... in the AtTOR-overexpressing ... lines
Deprost D, Yao L, Sormani R, Moreau M, Leterreux G, Nicolaï M, Bedu M, Robaglia C, Meyer C - The Arabidopsis TOR kinase links plant growth, yield, stress resistance and mRNA translation
Visit the PubMed ArticleCYCD3;1 expression is restricted to the ... very young primordia
Dewitte W, Scofield S, Alcasabas AA, Maughan SC, Menges M, Braun N, Collins C, Nieuwland J, Prinsen E, Sundaresan V, Murray JA - Arabidopsis CYCD3 D-type cyclins link cell proliferation and endocycles and are rate-limiting for cytokinin responses
Visit the PubMed ArticleCYCD3;2 ... reporters are also active in older leaf primordia
Dewitte W, Scofield S, Alcasabas AA, Maughan SC, Menges M, Braun N, Collins C, Nieuwland J, Prinsen E, Sundaresan V, Murray JA - Arabidopsis CYCD3 D-type cyclins link cell proliferation and endocycles and are rate-limiting for cytokinin responses
Visit the PubMed ArticleCYCD3;2 expression persisting longest in young leaves
Dewitte W, Scofield S, Alcasabas AA, Maughan SC, Menges M, Braun N, Collins C, Nieuwland J, Prinsen E, Sundaresan V, Murray JA - Arabidopsis CYCD3 D-type cyclins link cell proliferation and endocycles and are rate-limiting for cytokinin responses
Visit the PubMed Articleearlier decline in expression of the mitotic B-type cyclin CYCB1;1 in young cycd3;1-3 leaves
Dewitte W, Scofield S, Alcasabas AA, Maughan SC, Menges M, Braun N, Collins C, Nieuwland J, Prinsen E, Sundaresan V, Murray JA - Arabidopsis CYCD3 D-type cyclins link cell proliferation and endocycles and are rate-limiting for cytokinin responses
Visit the PubMed ArticleCYCD3;3 reporters are also active in older leaf primordia
Dewitte W, Scofield S, Alcasabas AA, Maughan SC, Menges M, Braun N, Collins C, Nieuwland J, Prinsen E, Sundaresan V, Murray JA - Arabidopsis CYCD3 D-type cyclins link cell proliferation and endocycles and are rate-limiting for cytokinin responses
Visit the PubMed ArticleCYCD3;1 expression is restricted to the ... young hydathodes
Dewitte W, Scofield S, Alcasabas AA, Maughan SC, Menges M, Braun N, Collins C, Nieuwland J, Prinsen E, Sundaresan V, Murray JA - Arabidopsis CYCD3 D-type cyclins link cell proliferation and endocycles and are rate-limiting for cytokinin responses
Visit the PubMed ArticleCYCD3;1 expression is restricted to the SAM
Dewitte W, Scofield S, Alcasabas AA, Maughan SC, Menges M, Braun N, Collins C, Nieuwland J, Prinsen E, Sundaresan V, Murray JA - Arabidopsis CYCD3 D-type cyclins link cell proliferation and endocycles and are rate-limiting for cytokinin responses
Visit the PubMed ArticleRNA gel blot analysis revealed that, whereas the expression of PAL1 ... was not significantly changed in siz1-3
Catala R, Ouyang J, Abreu IA, Hu Y, Seo H, Zhang X, Chua NH - The Arabidopsis E3 SUMO ligase SIZ1 regulates plant growth and drought responses
Visit the PubMed Articlein 3-week-old plants ... SIZ1 was not expressed ... in the basal region of developing young leaves
Catala R, Ouyang J, Abreu IA, Hu Y, Seo H, Zhang X, Chua NH - The Arabidopsis E3 SUMO ligase SIZ1 regulates plant growth and drought responses
Visit the PubMed ArticleFor example, under control conditions, SQS2 expression in the wild type was approximately threefold higher than in siz1-3 (Figure 5, panel 2, column WT C/siz1-3 C); however, the relative fold induction of this gene by drought was comparable in the mutant and the wild type
Catala R, Ouyang J, Abreu IA, Hu Y, Seo H, Zhang X, Chua NH - The Arabidopsis E3 SUMO ligase SIZ1 regulates plant growth and drought responses
Visit the PubMed Articlein siz1-3, the CHS1 transcript accumulation was clearly lower in the mutant compared with the wild type
Catala R, Ouyang J, Abreu IA, Hu Y, Seo H, Zhang X, Chua NH - The Arabidopsis E3 SUMO ligase SIZ1 regulates plant growth and drought responses
Visit the PubMed Articlein developing adult leaves, SIZ1 expression was detected in leaf blades and petioles
Catala R, Ouyang J, Abreu IA, Hu Y, Seo H, Zhang X, Chua NH - The Arabidopsis E3 SUMO ligase SIZ1 regulates plant growth and drought responses
Visit the PubMed Articlein 3-week-old plants ... SIZ1 was not expressed, at detectable levels, in juvenile leaves
Catala R, Ouyang J, Abreu IA, Hu Y, Seo H, Zhang X, Chua NH - The Arabidopsis E3 SUMO ligase SIZ1 regulates plant growth and drought responses
Visit the PubMed ArticleRNA gel blot analysis revealed that, whereas the expression of ... CHI was not significantly changed in siz1-3
Catala R, Ouyang J, Abreu IA, Hu Y, Seo H, Zhang X, Chua NH - The Arabidopsis E3 SUMO ligase SIZ1 regulates plant growth and drought responses
Visit the PubMed ArticleFigure 1 shows the representative expression profile of pSIZ1-2:GUS containing 3535 bp of 5′ upstream sequences ... In 3-d-old germinating seedlings, SIZ1 was expressed in all organs except part of the hypocotyl and the basal region of the cotyledons
Catala R, Ouyang J, Abreu IA, Hu Y, Seo H, Zhang X, Chua NH - The Arabidopsis E3 SUMO ligase SIZ1 regulates plant growth and drought responses
Visit the PubMed Articlein developing adult leaves, SIZ1 expression was detected in leaf blades and petioles
Catala R, Ouyang J, Abreu IA, Hu Y, Seo H, Zhang X, Chua NH - The Arabidopsis E3 SUMO ligase SIZ1 regulates plant growth and drought responses
Visit the PubMed ArticleIn the second category, exemplified by HMG1, basal level expression was reduced in siz1-3 compared with the wild type
Catala R, Ouyang J, Abreu IA, Hu Y, Seo H, Zhang X, Chua NH - The Arabidopsis E3 SUMO ligase SIZ1 regulates plant growth and drought responses
Visit the PubMed ArticleRT-PCR analysis of line E1439 confirmed that expression of DWF4 was absent in these plants
Reinhardt B, Hänggi E, Müller S, Bauch M, Wyrzykowska J, Kerstetter R, Poethig S, Fleming AJ - Restoration of DWF4 expression to the leaf margin of a dwf4 mutant is sufficient to restore leaf shape but not size: the role of the margin in leaf development
Visit the PubMed ArticleConfocal scanning laser microscopy of the E1439 line revealed that GFP expression was tightly restricted to the epidermal cell layer at the leaf perimeter
Reinhardt B, Hänggi E, Müller S, Bauch M, Wyrzykowska J, Kerstetter R, Poethig S, Fleming AJ - Restoration of DWF4 expression to the leaf margin of a dwf4 mutant is sufficient to restore leaf shape but not size: the role of the margin in leaf development
Visit the PubMed Articleour analysis revealed an accumulation of DWF4 mRNAin the epidermis of young leaf primordia
Reinhardt B, Hänggi E, Müller S, Bauch M, Wyrzykowska J, Kerstetter R, Poethig S, Fleming AJ - Restoration of DWF4 expression to the leaf margin of a dwf4 mutant is sufficient to restore leaf shape but not size: the role of the margin in leaf development
Visit the PubMed ArticleDWF4 transcripts ... became apparent in the vascular tissue
Reinhardt B, Hänggi E, Müller S, Bauch M, Wyrzykowska J, Kerstetter R, Poethig S, Fleming AJ - Restoration of DWF4 expression to the leaf margin of a dwf4 mutant is sufficient to restore leaf shape but not size: the role of the margin in leaf development
Visit the PubMed ArticleGFP expression observed in the E1439 driver line ... margin pattern is highlighted
Reinhardt B, Hänggi E, Müller S, Bauch M, Wyrzykowska J, Kerstetter R, Poethig S, Fleming AJ - Restoration of DWF4 expression to the leaf margin of a dwf4 mutant is sufficient to restore leaf shape but not size: the role of the margin in leaf development
Visit the PubMed ArticleDWF4::GUS reporter gene fusion under the control of the UAS promoter ... into the E1439 driver line ... Reporter gene activity was restricted to the leaf margin
Reinhardt B, Hänggi E, Müller S, Bauch M, Wyrzykowska J, Kerstetter R, Poethig S, Fleming AJ - Restoration of DWF4 expression to the leaf margin of a dwf4 mutant is sufficient to restore leaf shape but not size: the role of the margin in leaf development
Visit the PubMed ArticleDWF4 transcripts became more restricted to the ... abaxial epidermis
Reinhardt B, Hänggi E, Müller S, Bauch M, Wyrzykowska J, Kerstetter R, Poethig S, Fleming AJ - Restoration of DWF4 expression to the leaf margin of a dwf4 mutant is sufficient to restore leaf shape but not size: the role of the margin in leaf development
Visit the PubMed ArticleDWF4 transcripts became more restricted to the lateral ... epidermis
Reinhardt B, Hänggi E, Müller S, Bauch M, Wyrzykowska J, Kerstetter R, Poethig S, Fleming AJ - Restoration of DWF4 expression to the leaf margin of a dwf4 mutant is sufficient to restore leaf shape but not size: the role of the margin in leaf development
Visit the PubMed ArticleE1439>>DWF4 ... in situ hybridization analysis detected a low level of DWF4 transcripts only in a small group of cells around the margin of the complemented lines
Reinhardt B, Hänggi E, Müller S, Bauch M, Wyrzykowska J, Kerstetter R, Poethig S, Fleming AJ - Restoration of DWF4 expression to the leaf margin of a dwf4 mutant is sufficient to restore leaf shape but not size: the role of the margin in leaf development
Visit the PubMed ArticleOne of the lines identified by this screen (E1439) showed restriction of GFP expression within the leaf, primarily at the margin
Reinhardt B, Hänggi E, Müller S, Bauch M, Wyrzykowska J, Kerstetter R, Poethig S, Fleming AJ - Restoration of DWF4 expression to the leaf margin of a dwf4 mutant is sufficient to restore leaf shape but not size: the role of the margin in leaf development
Visit the PubMed ArticleMID:GUS ... Expression was found in the actively proliferating tissues of the shoot and root
Kirik V, Schrader A, Uhrig JF, Hulskamp M - MIDGET unravels functions of the Arabidopsis topoisomerase VI complex in DNA endoreduplication, chromatin condensation, and transcriptional silencing
Visit the PubMed ArticleMID:GUS ... We also found expression in elongating hypocotyl cells and developing trichomes
Kirik V, Schrader A, Uhrig JF, Hulskamp M - MIDGET unravels functions of the Arabidopsis topoisomerase VI complex in DNA endoreduplication, chromatin condensation, and transcriptional silencing
Visit the PubMed ArticleTSI expression is activated in the mid ... mutants
Kirik V, Schrader A, Uhrig JF, Hulskamp M - MIDGET unravels functions of the Arabidopsis topoisomerase VI complex in DNA endoreduplication, chromatin condensation, and transcriptional silencing
Visit the PubMed ArticleWe analyzed the expression of the pCYCB1;1:GUS reporter in the mid mutants (Colon-Carmona et al., 1999) and found expression not only in mitotic cells but also in differentiated cells, including cells that have endoreduplicated DNA-like trichomes and hypocotyl cells
Kirik V, Schrader A, Uhrig JF, Hulskamp M - MIDGET unravels functions of the Arabidopsis topoisomerase VI complex in DNA endoreduplication, chromatin condensation, and transcriptional silencing
Visit the PubMed ArticleTSI expression is activated in the ... fas1 mutants
Kirik V, Schrader A, Uhrig JF, Hulskamp M - MIDGET unravels functions of the Arabidopsis topoisomerase VI complex in DNA endoreduplication, chromatin condensation, and transcriptional silencing
Visit the PubMed ArticleIn 35S:MID-YFP lines, a strong YFP fluorescence signal was found in the nuclei in different cell types, indicating that MID is a nuclear protein
Kirik V, Schrader A, Uhrig JF, Hulskamp M - MIDGET unravels functions of the Arabidopsis topoisomerase VI complex in DNA endoreduplication, chromatin condensation, and transcriptional silencing
Visit the PubMed ArticleCYC1;4 ... does not appear to differ between the wild type and bin4
Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis
Visit the PubMed ArticleBIN4 promoter ... GUS signal is also detectable in various tissues that contain proliferating cells, such as root and shoot apical meristems
Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis
Visit the PubMed ArticleIn addition, our real-time RT-PCR analysis revealed that several DSB-inducible genes, including poly (ADP-ribose) polymerase1 (At PARP1 ... were significantly upregulated in bin4
Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis
Visit the PubMed ArticleThe publicly available microarray data, using synchronized Arabidopsis cell cultures (Menges et al., 2005), revealed that BIN4 is ubiquitously expressed at low levels during the mitotic cell cycle (see Supplemental Figure 5 online). This pattern of expression is similar to that of other components of the plant topo VI complex ... i.e ... TOP6B/RHL3/HYP6/BIN3
Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis
Visit the PubMed ArticleIndeed, we found that the BIN4:GFP (for green fluorescent protein) fusion protein, expressed under the control of a cauliflower mosaic virus 35S promoter in Arabidopsis leaf epidermal cells, is targeted to the nucleus
Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis
Visit the PubMed ArticleWEE1 ... does not appear to differ between the wild type and bin4
Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis
Visit the PubMed ArticleCYCA1;1 ... does not appear to differ between the wild type and bin4
Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis
Visit the PubMed Articlehistone H4 ... does not appear to differ between the wild type and bin4
Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis
Visit the PubMed Articleour real-time RT-PCR analyses showed that the transcript level of a G2/M-specific gene, cyclin B1;1 (CYCB1;1), was increased significantly in bin4-1
Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis
Visit the PubMed ArticleCYCB2;3 ... does not appear to differ between the wild type and bin4
Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis
Visit the PubMed ArticleCYCB1;2 ... does not appear to differ between the wild type and bin4
Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis
Visit the PubMed Articleaddition, our real-time RT-PCR analysis revealed that several DSB-inducible genes, including ... breast cancer susceptibility1 (At BRCA1) ... were significantly upregulated in bin4
Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis
Visit the PubMed ArticleWe examined the developmental pattern of the BIN4 promoter activity by fusing the 2-kb genomic region upstream of the BIN4 initiation codon to the β-glucuronidase (GUS) reporter gene. The histochemical analysis revealed that the BIN4 promoter is active in endoreduplicating cells, including those in young, rapidly expanding cotyledons
Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis
Visit the PubMed ArticleThe publicly available microarray data, using synchronized Arabidopsis cell cultures (Menges et al., 2005), revealed that BIN4 is ubiquitously expressed at low levels during the mitotic cell cycle (see Supplemental Figure 5 online). This pattern of expression is similar to that of other components of the plant topo VI complex ... i.e., RHL1/HYP7
Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis
Visit the PubMed ArticleThe publicly available microarray data, using synchronized Arabidopsis cell cultures (Menges et al., 2005), revealed that BIN4 is ubiquitously expressed at low levels during the mitotic cell cycle
Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis
Visit the PubMed ArticleThe publicly available microarray data, using synchronized Arabidopsis cell cultures (Menges et al., 2005), revealed that BIN4 is ubiquitously expressed at low levels during the mitotic cell cycle (see Supplemental Figure 5 online). This pattern of expression is similar to that of other components of the plant topo VI complex ... i.e ... SPO11-3/RHL2/BIN5
Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis
Visit the PubMed ArticleCYCD4;1 ... does not appear to differ between the wild type and bin4
Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis
Visit the PubMed ArticleIn addition, our real-time RT-PCR analysis revealed that several DSB-inducible genes, including ... RAD51 ... were significantly upregulated in bin4
Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis
Visit the PubMed ArticleTo further explore the spatial pattern of ectopic CYCB1;1 expression in bin4, we introduced the CYCB1;1:GUS construct (Colon-Carmona et al., 1999) into the bin4-1 background and examined the accumulation of CYCB1;1 transcripts. In the wild type, CYCB1;1 was predominantly expressed in proliferating tissues, and very little GUS signal was detected in mature tissues (Colon-Carmona et al., 1999) (Figures 7B, 7D, 7F, 7H, and 7J). We detected similar, or slightly reduced, levels of CYCB1;1 expression in the bin4 root meristems (Figure 7C), suggesting that bin4 is not greatly impaired in G2/M progression during the mitotic cell cycle. Unexpectedly, however, we found strong CYCB1;1 accumulation in nondividing cells, including those in light- and dark-grown hypocotyls (Figures 7E and 7G, respectively), cotyledons (Figure 7I), the mature part of roots (Figure 7K), leaf trichomes (Figure 7L), and leaf marginal cells
Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis
Visit the PubMed ArticleIn addition, our real-time RT-PCR analysis revealed that several DSB-inducible genes, including ... At PARP2 ... were significantly upregulated in bin4
Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis
Visit the PubMed ArticleCYCB2;1 ... does not appear to differ between the wild type and bin4
Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis
Visit the PubMed ArticleWe further investigated the expression pattern of At PARP2 by introducing the pPARP2:GUS construct (Babiychuk et al., 1998) into the bin4-1 background. The histochemical analysis revealed that the At PARP2 gene is normally expressed at an undetectable level (Figures 5B, 5F, 5H, 5J, and 5L). To our surprise, however, we found that the bin4 mutation induces the expression of At PARP2 predominantly in postmeristematic cells ... In addition, we detected similar ectopic expression of At PARP2 in other postproliferating tissues, including light- and dark-grown hypocotyls (Figures 5G and 5I, respectively), cotyledons (Figure 5K), mature part of roots (Figure 5M), leaf trichomes (Figure 5N), and leaf marginal cells (Figure 5O), in bin4. These results suggest that the induced transcriptional activation of DNA damage response genes is restricted to the nonproliferating cells that have undergone several endocycles
Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis
Visit the PubMed ArticleBIN4 promoter is active in endoreduplicating cells, including ... vascular cells (Figure 4C), elongating root cells (Figures 4E and 4H), and developing leaf trichomes
Breuer C, Stacey NJ, West CE, Zhao Y, Chory J, Tsukaya H, Azumi Y, Maxwell A, Roberts K, Sugimoto-Shirasu K - BIN4, a novel component of the plant DNA topoisomerase VI complex, is required for endoreduplication in Arabidopsis
Visit the PubMed ArticleKLU is expressed ... at the base of developing leaves
Anastasiou E, Kenz S, Gerstung M, MacLean D, Timmer J, Fleck C, Lenhard M - Control of plant organ size by KLUH/CYP78A5-dependent intercellular signaling
Visit the PubMed ArticleTo localize KLU protein, we expressed a KLU-vYFP fusion protein under the control of the KLU promoter. The fusion protein fully complemented the klu mutant phenotype, demonstrating its functionality (Figure S2I). Intracellularly, vYFP fluorescence was detected in a ring around the nucleus and in a mesh-like pattern in the cytoplasm (Figure 4I), which is typical for ER-associated proteins
Anastasiou E, Kenz S, Gerstung M, MacLean D, Timmer J, Fleck C, Lenhard M - Control of plant organ size by KLUH/CYP78A5-dependent intercellular signaling
Visit the PubMed ArticleWhen comparing the regions of KLU expression and cell division in young leaves (Figures 4A and 4B) and in petals at the stage that is most sensitive to KLU activity (Figures 4E′ and 4F′; cf. above), it is apparent that KLU is expressed outside the region of proliferation. Of note, KLU expression is still detected when cells have already ceased to proliferate (Figures 4E″ and 4F″), suggesting that it is not the downregulation of KLU expression that causes the eventual arrest of proliferation
Anastasiou E, Kenz S, Gerstung M, MacLean D, Timmer J, Fleck C, Lenhard M - Control of plant organ size by KLUH/CYP78A5-dependent intercellular signaling
Visit the PubMed ArticleKLU is expressed ... at the boundary of the shoot and lateral organs
Anastasiou E, Kenz S, Gerstung M, MacLean D, Timmer J, Fleck C, Lenhard M - Control of plant organ size by KLUH/CYP78A5-dependent intercellular signaling
Visit the PubMed ArticleKLU is expressed on the flanks of the shoot meristem
Anastasiou E, Kenz S, Gerstung M, MacLean D, Timmer J, Fleck C, Lenhard M - Control of plant organ size by KLUH/CYP78A5-dependent intercellular signaling
Visit the PubMed ArticleWe generated C-terminal and N-terminal GFP translational fusion genomic rescue constructs: pSEU::GFP:SEU and pSEU::SEU:GFP ... For all transformants, GFP florescence was detected in all tissues examined ... vegetative ... SAM
Azhakanandam S, Nole-Wilson S, Bao F, Franks RG - SEUSS and AINTEGUMENTA mediate patterning and ovule initiation during gynoecium medial domain development
Visit the PubMed ArticleWe generated C-terminal and N-terminal GFP translational fusion genomic rescue constructs: pSEU::GFP:SEU and pSEU::SEU:GFP ... For all transformants, GFP florescence was detected in all tissues examined ... young leaf
Azhakanandam S, Nole-Wilson S, Bao F, Franks RG - SEUSS and AINTEGUMENTA mediate patterning and ovule initiation during gynoecium medial domain development
Visit the PubMed ArticleAtEXP3 was induced by IAA only ... in wild type seedlings
Kwon YR, Lee HJ, Kim KH, Hong SW, Lee SJ, Lee H - Ectopic expression of Expansin3 or Expansinbeta1 causes enhanced hormone and salt stress sensitivity in Arabidopsis
Visit the PubMed ArticleAtEXPβ1 was not induced by IAA in wild type seedlings
Kwon YR, Lee HJ, Kim KH, Hong SW, Lee SJ, Lee H - Ectopic expression of Expansin3 or Expansinbeta1 causes enhanced hormone and salt stress sensitivity in Arabidopsis
Visit the PubMed ArticleAtEXPβ1 was induced by ACC only in wild type seedlings
Kwon YR, Lee HJ, Kim KH, Hong SW, Lee SJ, Lee H - Ectopic expression of Expansin3 or Expansinbeta1 causes enhanced hormone and salt stress sensitivity in Arabidopsis
Visit the PubMed ArticleA DA1 promoter::GUS fusion (pDA1::GUS) was expressed most strongly during the early stages of leaf, petal, integument, and embryo formation (Fig. 4D–H) and at much reduced levels later in petal and leaf development, consistent with its role in controlling the period of cell proliferation
Li Y, Zheng L, Corke F, Smith C, Bevan MW - Control of final seed and organ size by the DA1 gene family in Arabidopsis thaliana
Visit the PubMed ArticleMicroarray data showed that DA1 is expressed at higher levels in ABA-treated seedlings (Li et al. 2006), and further experiments established that DA1 is slowly induced by ABA (Fig. 4L) and not by other growth regulators such as jasmonic acid, auxin, cytokinin, brassinosteroids, gibberellins, or glucose
Li Y, Zheng L, Corke F, Smith C, Bevan MW - Control of final seed and organ size by the DA1 gene family in Arabidopsis thaliana
Visit the PubMed ArticleTo assess the subcellular distribution of UBP15, constructs containing either 35S:GFP-UBP15 or 35S:UBP15-GFP were transformed into onion epidermal cells. Transient expression showed that the fusion proteins were ubiquitously present in both the cytosol and nucleus
Liu Y, Wang F, Zhang H, He H, Ma L, Deng XW - Functional characterization of the Arabidopsis ubiquitin-specific protease gene family reveals specific role and redundancy of individual members in development
Visit the PubMed ArticleTo assess the subcellular distribution of UBP15, constructs containing either 35S:GFP-UBP15 or 35S:UBP15-GFP were transformed into onion epidermal cells. Transient expression showed that the fusion proteins were ubiquitously present in both the cytosol and nucleus
Liu Y, Wang F, Zhang H, He H, Ma L, Deng XW - Functional characterization of the Arabidopsis ubiquitin-specific protease gene family reveals specific role and redundancy of individual members in development
Visit the PubMed Articlein ubp15-1 ... ICK1 was up-regulated
Liu Y, Wang F, Zhang H, He H, Ma L, Deng XW - Functional characterization of the Arabidopsis ubiquitin-specific protease gene family reveals specific role and redundancy of individual members in development
Visit the PubMed Articlein ubp15-1 ... Expression of the CAL gene ... was slightly increased (by 1.98-fold)
Liu Y, Wang F, Zhang H, He H, Ma L, Deng XW - Functional characterization of the Arabidopsis ubiquitin-specific protease gene family reveals specific role and redundancy of individual members in development
Visit the PubMed Articlein ubp15-1 ... Cyclin-like F-box domain-containing protein was down-regulated
Liu Y, Wang F, Zhang H, He H, Ma L, Deng XW - Functional characterization of the Arabidopsis ubiquitin-specific protease gene family reveals specific role and redundancy of individual members in development
Visit the PubMed Articlein ubp15-1 ... expression of MAF5 ... decreased by 0.31-fold
Liu Y, Wang F, Zhang H, He H, Ma L, Deng XW - Functional characterization of the Arabidopsis ubiquitin-specific protease gene family reveals specific role and redundancy of individual members in development
Visit the PubMed Articlethe mRNA level of UBP15 was higher in rosette leaves and inflorescences than in other organs ... We further examined the spatial expression pattern of UBP15 within a rosette leaf at various different developmental stages. Interestingly, the UBP15 mRNA level increased from the early to late leaf stages, with higher expression in the leaf margin in the late stage
Liu Y, Wang F, Zhang H, He H, Ma L, Deng XW - Functional characterization of the Arabidopsis ubiquitin-specific protease gene family reveals specific role and redundancy of individual members in development
Visit the PubMed ArticleWe found that there were substantially fewer Histone H4–expressing cells in the ProSPL9:rSPL9 shoot apical meristem
Wang JW, Schwab R, Czech B, Mica E, Weigel D - Dual effects of miR156-targeted SPL genes and CYP78A5/KLUH on plastochron length and organ size in Arabidopsis thaliana
Visit the PubMed ArticleThere was no significant change of CYP78A5 transcript levels in ... Pro35S:MIR156f ... plants
Wang JW, Schwab R, Czech B, Mica E, Weigel D - Dual effects of miR156-targeted SPL genes and CYP78A5/KLUH on plastochron length and organ size in Arabidopsis thaliana
Visit the PubMed ArticleCYP78A5/KLUH ... expressed at the periphery of the meristem proper
Wang JW, Schwab R, Czech B, Mica E, Weigel D - Dual effects of miR156-targeted SPL genes and CYP78A5/KLUH on plastochron length and organ size in Arabidopsis thaliana
Visit the PubMed ArticleSPL9 mRNA was found in leaf anlagen and in developing leaf primordia
Wang JW, Schwab R, Czech B, Mica E, Weigel D - Dual effects of miR156-targeted SPL genes and CYP78A5/KLUH on plastochron length and organ size in Arabidopsis thaliana
Visit the PubMed Articledirect analysis of miR156 expression by in situ hybridization, which revealed that miR156 accumulated ... in ... leaf primordia
Wang JW, Schwab R, Czech B, Mica E, Weigel D - Dual effects of miR156-targeted SPL genes and CYP78A5/KLUH on plastochron length and organ size in Arabidopsis thaliana
Visit the PubMed ArticleSPL9 levels were unaffected by cyp78a5
Wang JW, Schwab R, Czech B, Mica E, Weigel D - Dual effects of miR156-targeted SPL genes and CYP78A5/KLUH on plastochron length and organ size in Arabidopsis thaliana
Visit the PubMed ArticleAs young leaves grew, SPL9 expression became gradually re- stricted to vascular tissues
Wang JW, Schwab R, Czech B, Mica E, Weigel D - Dual effects of miR156-targeted SPL genes and CYP78A5/KLUH on plastochron length and organ size in Arabidopsis thaliana
Visit the PubMed Articledirect analysis of miR156 expression by in situ hybridization, which revealed that miR156 accumulated ... in ... shoot apical meristem proper
Wang JW, Schwab R, Czech B, Mica E, Weigel D - Dual effects of miR156-targeted SPL genes and CYP78A5/KLUH on plastochron length and organ size in Arabidopsis thaliana
Visit the PubMed ArticleIn Pro35S:MIR156f plants, SPL9 transcripts were strongly reduced
Wang JW, Schwab R, Czech B, Mica E, Weigel D - Dual effects of miR156-targeted SPL genes and CYP78A5/KLUH on plastochron length and organ size in Arabidopsis thaliana
Visit the PubMed Articlewe did not detect any SPL9 transcripts in the central part of the meristem
Wang JW, Schwab R, Czech B, Mica E, Weigel D - Dual effects of miR156-targeted SPL genes and CYP78A5/KLUH on plastochron length and organ size in Arabidopsis thaliana
Visit the PubMed ArticleThere was no significant change of CYP78A5 transcript levels in ... ProSPL9:rSPL9 plants
Wang JW, Schwab R, Czech B, Mica E, Weigel D - Dual effects of miR156-targeted SPL genes and CYP78A5/KLUH on plastochron length and organ size in Arabidopsis thaliana
Visit the PubMed ArticleWe therefore analyzed SPL9 expression in two mutants with reduced miR156 levels, se-1 and ago1-27. Compared with the wild type, SPL9 RNA levels were substantially elevated in both mutants. However, the overall pattern of expression was similar to that seen in the wild type
Wang JW, Schwab R, Czech B, Mica E, Weigel D - Dual effects of miR156-targeted SPL genes and CYP78A5/KLUH on plastochron length and organ size in Arabidopsis thaliana
Visit the PubMed ArticleWe therefore analyzed SPL9 expression in two mutants with reduced miR156 levels, se-1 and ago1-27. Compared with the wild type, SPL9 RNA levels were substantially elevated in both mutants. However, the overall pattern of expression was similar to that seen in the wild type
Wang JW, Schwab R, Czech B, Mica E, Weigel D - Dual effects of miR156-targeted SPL genes and CYP78A5/KLUH on plastochron length and organ size in Arabidopsis thaliana
Visit the PubMed Articlewe employed transgenic plants in which miR156 activity is specifically reduced by con- stitutive expression of a target mimic (Franco-Zorrilla et al., 2007). SPL9 mRNA expression was changed in these Pro35S: MIM156 plants in a similar manner as in se-1 and ago1-27 plants
Wang JW, Schwab R, Czech B, Mica E, Weigel D - Dual effects of miR156-targeted SPL genes and CYP78A5/KLUH on plastochron length and organ size in Arabidopsis thaliana
Visit the PubMed ArticleCYP78A5/KLUH ... expressed ... at the base of new leaf primordia
Wang JW, Schwab R, Czech B, Mica E, Weigel D - Dual effects of miR156-targeted SPL genes and CYP78A5/KLUH on plastochron length and organ size in Arabidopsis thaliana
Visit the PubMed ArticleCol CPL2pro:CPL2-GUS seedlings ... In shoots, GUS activity was particularly strong in ... shoot meristems
Ueda A, Li P, Feng Y, Vikram M, Kim S, Kang CH, Kang JS, Bahk JD, Lee SY, Fukuhara T, Staswick PE, Pepper AE, Koiwa H - The Arabidopsis thaliana carboxyl-terminal domain phosphatase-like 2 regulates plant growth, stress and auxin responses
Visit the PubMed ArticleIn Col CPL2pro:CPL2-GUS seedlings, CPL2-GUS expression was observed throughout the plants (Fig. 2b). In later stages, GUS activity was associated with tissues undergoing cell division and expansion. In shoots, GUS activity was particularly strong in expanding leaves
Ueda A, Li P, Feng Y, Vikram M, Kim S, Kang CH, Kang JS, Bahk JD, Lee SY, Fukuhara T, Staswick PE, Pepper AE, Koiwa H - The Arabidopsis thaliana carboxyl-terminal domain phosphatase-like 2 regulates plant growth, stress and auxin responses
Visit the PubMed ArticleIn order to determine the level of auxin responsiveness of whole plants, DR5-GUS expressions in wild type and cpl2 was determined using a histochemical GUS assay. GUS activity in untreated cpl2-2 leaves was very low, whereas its expression level in the roots was similar to wild type (Fig. 8a). Consistently, root growth responses and lateral root development of wild type and cpl2 alleles to exogenously supplied auxin were indistinguishable
Ueda A, Li P, Feng Y, Vikram M, Kim S, Kang CH, Kang JS, Bahk JD, Lee SY, Fukuhara T, Staswick PE, Pepper AE, Koiwa H - The Arabidopsis thaliana carboxyl-terminal domain phosphatase-like 2 regulates plant growth, stress and auxin responses
Visit the PubMed ArticleUnlike fry2-1, RD29a-LUC expression in cpl2-2 was specifically hyper-responsive to high NaCl treatment (Fig. 4a), but its cold and ABA induction was not as pronounced as in fry2-1
Ueda A, Li P, Feng Y, Vikram M, Kim S, Kang CH, Kang JS, Bahk JD, Lee SY, Fukuhara T, Staswick PE, Pepper AE, Koiwa H - The Arabidopsis thaliana carboxyl-terminal domain phosphatase-like 2 regulates plant growth, stress and auxin responses
Visit the PubMed ArticleCol CPL2pro:CPL2-GUS seedlings ... cotyledons were stained only in ... petioles
Ueda A, Li P, Feng Y, Vikram M, Kim S, Kang CH, Kang JS, Bahk JD, Lee SY, Fukuhara T, Staswick PE, Pepper AE, Koiwa H - The Arabidopsis thaliana carboxyl-terminal domain phosphatase-like 2 regulates plant growth, stress and auxin responses
Visit the PubMed ArticleBasal expression level of DR5-GUS in cpl2-2 seedlings was 28% of wild type seedlings (Fig. 6). This is consistent with the data obtained with GUS activity based on MUG assay (data not shown).
Ueda A, Li P, Feng Y, Vikram M, Kim S, Kang CH, Kang JS, Bahk JD, Lee SY, Fukuhara T, Staswick PE, Pepper AE, Koiwa H - The Arabidopsis thaliana carboxyl-terminal domain phosphatase-like 2 regulates plant growth, stress and auxin responses
Visit the PubMed Articlebasal expression levels of IAA1 ... were decreased in cpl2-2 mutant plants
Ueda A, Li P, Feng Y, Vikram M, Kim S, Kang CH, Kang JS, Bahk JD, Lee SY, Fukuhara T, Staswick PE, Pepper AE, Koiwa H - The Arabidopsis thaliana carboxyl-terminal domain phosphatase-like 2 regulates plant growth, stress and auxin responses
Visit the PubMed Articlebasal expression levels of ... IAA19 were decreased in cpl2-2 mutant plants
Ueda A, Li P, Feng Y, Vikram M, Kim S, Kang CH, Kang JS, Bahk JD, Lee SY, Fukuhara T, Staswick PE, Pepper AE, Koiwa H - The Arabidopsis thaliana carboxyl-terminal domain phosphatase-like 2 regulates plant growth, stress and auxin responses
Visit the PubMed ArticleCol CPL2pro:CPL2-GUS seedlings ... cotyledons were stained only in vein tissues
Ueda A, Li P, Feng Y, Vikram M, Kim S, Kang CH, Kang JS, Bahk JD, Lee SY, Fukuhara T, Staswick PE, Pepper AE, Koiwa H - The Arabidopsis thaliana carboxyl-terminal domain phosphatase-like 2 regulates plant growth, stress and auxin responses
Visit the PubMed ArticleHOG1 OE ... lines ... ARR5 ... 3- to 9-fold decrease in the expression levels
Godge MR, Kumar D, Kumar PP - Arabidopsis HOG1 gene and its petunia homolog PETCBP act as key regulators of yield parameters
Visit the PubMed ArticleWe performed qRT-PCR analysis with RNA from seedlings of ... HOG1 ... AS lines ... The transcript levels of KNAT1 ... were significantly upregulated by 6- to 8-fold even without BA treatment
Godge MR, Kumar D, Kumar PP - Arabidopsis HOG1 gene and its petunia homolog PETCBP act as key regulators of yield parameters
Visit the PubMed ArticleHOG1 ... AS lines ... ARR4 ... higher level (4- to 9-fold increase)
Godge MR, Kumar D, Kumar PP - Arabidopsis HOG1 gene and its petunia homolog PETCBP act as key regulators of yield parameters
Visit the PubMed ArticleHOG1 OE ... lines ... ARR6 ... 3- to 9-fold decrease in the expression levels
Godge MR, Kumar D, Kumar PP - Arabidopsis HOG1 gene and its petunia homolog PETCBP act as key regulators of yield parameters
Visit the PubMed ArticleARR5 ... were upregulated within minutes after a single cytokinin pulse treatment
Godge MR, Kumar D, Kumar PP - Arabidopsis HOG1 gene and its petunia homolog PETCBP act as key regulators of yield parameters
Visit the PubMed ArticleHOG1 OE ... lines ... ARR4 ... 3- to 9-fold decrease in the expression levels
Godge MR, Kumar D, Kumar PP - Arabidopsis HOG1 gene and its petunia homolog PETCBP act as key regulators of yield parameters
Visit the PubMed ArticleHOG1 ... AS lines ... ARR5 ... higher level (4- to 9-fold increase)
Godge MR, Kumar D, Kumar PP - Arabidopsis HOG1 gene and its petunia homolog PETCBP act as key regulators of yield parameters
Visit the PubMed ArticleExpression analysis of different plant parts indicated that HOG1 (Fig. 2d) and HOG2 (Fig. 2a) are constitutively expressed in Arabidopsis, with relatively higher levels in the leaves and inflorescence stem
Godge MR, Kumar D, Kumar PP - Arabidopsis HOG1 gene and its petunia homolog PETCBP act as key regulators of yield parameters
Visit the PubMed ArticleHOG1 ... AS lines ... ARR6 ... higher level (4- to 9-fold increase)
Godge MR, Kumar D, Kumar PP - Arabidopsis HOG1 gene and its petunia homolog PETCBP act as key regulators of yield parameters
Visit the PubMed ArticleApplication of BA caused a dose-dependent increase of ... STM transcripts
Godge MR, Kumar D, Kumar PP - Arabidopsis HOG1 gene and its petunia homolog PETCBP act as key regulators of yield parameters
Visit the PubMed ArticleWe performed qRT-PCR analysis with RNA from seedlings of ... HOG1 ... AS lines ... The transcript levels of ... STM were significantly upregulated by 6- to 8-fold even without BA treatment
Godge MR, Kumar D, Kumar PP - Arabidopsis HOG1 gene and its petunia homolog PETCBP act as key regulators of yield parameters
Visit the PubMed ArticleARR6 ... were upregulated within minutes after a single cytokinin pulse treatment
Godge MR, Kumar D, Kumar PP - Arabidopsis HOG1 gene and its petunia homolog PETCBP act as key regulators of yield parameters
Visit the PubMed ArticleARR4 ... were upregulated within minutes after a single cytokinin pulse treatment
Godge MR, Kumar D, Kumar PP - Arabidopsis HOG1 gene and its petunia homolog PETCBP act as key regulators of yield parameters
Visit the PubMed ArticleExpression analysis of different plant parts indicated that HOG1 (Fig. 2d) and HOG2 (Fig. 2a) are constitutively expressed in Arabidopsis, with relatively higher levels in the leaves and inflorescence stem
Godge MR, Kumar D, Kumar PP - Arabidopsis HOG1 gene and its petunia homolog PETCBP act as key regulators of yield parameters
Visit the PubMed ArticleApplication of BA caused a dose-dependent increase of KNAT1
Godge MR, Kumar D, Kumar PP - Arabidopsis HOG1 gene and its petunia homolog PETCBP act as key regulators of yield parameters
Visit the PubMed ArticleHOG1 OE ... lines ... showed 4- to 7-fold decrease of ... STM transcripts
Godge MR, Kumar D, Kumar PP - Arabidopsis HOG1 gene and its petunia homolog PETCBP act as key regulators of yield parameters
Visit the PubMed ArticleHOG1 OE ... lines ... showed 4- to 7-fold decrease of ... KNAT1
Godge MR, Kumar D, Kumar PP - Arabidopsis HOG1 gene and its petunia homolog PETCBP act as key regulators of yield parameters
Visit the PubMed ArticleFor IAA28 overexpressing lines, the IAA13 transcription level was not altered
Falkenberg B, Witt I, Zanor MI, Steinhauser D, Mueller-Roeber B, Hesse H, Hoefgen R - Transcription factors relevant to auxin signalling coordinate broad-spectrum metabolic shifts including sulphur metabolism
Visit the PubMed ArticleFor IAA28 overexpressing lines ... the ARF1-BP transcript was down-regulated by up to 88%
Falkenberg B, Witt I, Zanor MI, Steinhauser D, Mueller-Roeber B, Hesse H, Hoefgen R - Transcription factors relevant to auxin signalling coordinate broad-spectrum metabolic shifts including sulphur metabolism
Visit the PubMed ArticleIAA28 ... up-regulated during sulphur starvation in Arabidopsis thaliana seedlings
Falkenberg B, Witt I, Zanor MI, Steinhauser D, Mueller-Roeber B, Hesse H, Hoefgen R - Transcription factors relevant to auxin signalling coordinate broad-spectrum metabolic shifts including sulphur metabolism
Visit the PubMed ArticleIn ARF1-BP overexpressing lines ... IAA13 transcript accumulated ... 2-fold
Falkenberg B, Witt I, Zanor MI, Steinhauser D, Mueller-Roeber B, Hesse H, Hoefgen R - Transcription factors relevant to auxin signalling coordinate broad-spectrum metabolic shifts including sulphur metabolism
Visit the PubMed ArticleIn IAA13 overexpressing lines ... ARF1-BP transcript was reduced by 86%
Falkenberg B, Witt I, Zanor MI, Steinhauser D, Mueller-Roeber B, Hesse H, Hoefgen R - Transcription factors relevant to auxin signalling coordinate broad-spectrum metabolic shifts including sulphur metabolism
Visit the PubMed ArticleIAA13 ... up-regulated during sulphur starvation in Arabidopsis thaliana seedlings
Falkenberg B, Witt I, Zanor MI, Steinhauser D, Mueller-Roeber B, Hesse H, Hoefgen R - Transcription factors relevant to auxin signalling coordinate broad-spectrum metabolic shifts including sulphur metabolism
Visit the PubMed ArticleIn ARF1-BP overexpressing lines ... IAA28 ... transcript accumulated 3-fold
Falkenberg B, Witt I, Zanor MI, Steinhauser D, Mueller-Roeber B, Hesse H, Hoefgen R - Transcription factors relevant to auxin signalling coordinate broad-spectrum metabolic shifts including sulphur metabolism
Visit the PubMed ArticleARF2 ... up-regulated during sulphur starvation in Arabidopsis thaliana seedlings
Falkenberg B, Witt I, Zanor MI, Steinhauser D, Mueller-Roeber B, Hesse H, Hoefgen R - Transcription factors relevant to auxin signalling coordinate broad-spectrum metabolic shifts including sulphur metabolism
Visit the PubMed ArticleIn seedlings expressing the GUS protein under the control of the DOT1 promoter, expression was detected in emerging leaf primordia and young leaves (Figure S5
Petricka JJ, Clay NK, Nelson TM - Vein patterning screens and the defectively organized tributaries mutants in Arabidopsis thaliana
Visit the PubMed ArticleA DOT3 promoter fused to GUS was detected in emerging leaf primordia (Figure S5
Petricka JJ, Clay NK, Nelson TM - Vein patterning screens and the defectively organized tributaries mutants in Arabidopsis thaliana
Visit the PubMed ArticleExpression of a DOT2 promoter-GUS fusion can be seen in young leaves (Figure S5
Petricka JJ, Clay NK, Nelson TM - Vein patterning screens and the defectively organized tributaries mutants in Arabidopsis thaliana
Visit the PubMed ArticleVery weak expression in leaves was detected in DOT4 promoter GUS lines (Figure S5
Petricka JJ, Clay NK, Nelson TM - Vein patterning screens and the defectively organized tributaries mutants in Arabidopsis thaliana
Visit the PubMed Articlegenes induced by threefold or more in BA-treated wild-type shoots ... includes type A ARRs, the cytokinin receptor AHK4
Argyros RD, Mathews DE, Chiang YH, Palmer CM, Thibault DM, Etheridge N, Argyros DA, Mason MG, Kieber JJ, Schaller GE - Type B response regulators of Arabidopsis play key roles in cytokinin signaling and plant development
Visit the PubMed ArticleAHK4 ... in the arr1-3 arr10-5 arr12-1 mutant ... basal expression level was reduced compared with that in the wild type, indicating a reduced response to endogenous cytokinin in the mutant
Argyros RD, Mathews DE, Chiang YH, Palmer CM, Thibault DM, Etheridge N, Argyros DA, Mason MG, Kieber JJ, Schaller GE - Type B response regulators of Arabidopsis play key roles in cytokinin signaling and plant development
Visit the PubMed ArticleARR15 ... in the arr1-3 arr10-5 arr12-1 mutant ... basal expression level was reduced compared with that in the wild type, indicating a reduced response to endogenous cytokinin in the mutant
Argyros RD, Mathews DE, Chiang YH, Palmer CM, Thibault DM, Etheridge N, Argyros DA, Mason MG, Kieber JJ, Schaller GE - Type B response regulators of Arabidopsis play key roles in cytokinin signaling and plant development
Visit the PubMed ArticleCKX4 in the arr1-3 arr10-5 arr12-1 mutant ... basal expression level was reduced compared with that in the wild type, indicating a reduced response to endogenous cytokinin in the mutant
Argyros RD, Mathews DE, Chiang YH, Palmer CM, Thibault DM, Etheridge N, Argyros DA, Mason MG, Kieber JJ, Schaller GE - Type B response regulators of Arabidopsis play key roles in cytokinin signaling and plant development
Visit the PubMed ArticleARR7 ... the arr1-3 arr10-5 arr12-1 mutant ... basal expression level was reduced compared with that in the wild type, indicating a reduced response to endogenous cytokinin in the mutant
Argyros RD, Mathews DE, Chiang YH, Palmer CM, Thibault DM, Etheridge N, Argyros DA, Mason MG, Kieber JJ, Schaller GE - Type B response regulators of Arabidopsis play key roles in cytokinin signaling and plant development
Visit the PubMed ArticleARR5 ... in the arr1-3 arr10-5 arr12-1 mutant ... basal expression level was reduced compared with that in the wild type, indicating a reduced response to endogenous cytokinin in the mutant
Argyros RD, Mathews DE, Chiang YH, Palmer CM, Thibault DM, Etheridge N, Argyros DA, Mason MG, Kieber JJ, Schaller GE - Type B response regulators of Arabidopsis play key roles in cytokinin signaling and plant development
Visit the PubMed Articlegenes induced by threefold or more in BA-treated wild-type shoots ... includes ... cytokinin oxidase CKX4
Argyros RD, Mathews DE, Chiang YH, Palmer CM, Thibault DM, Etheridge N, Argyros DA, Mason MG, Kieber JJ, Schaller GE - Type B response regulators of Arabidopsis play key roles in cytokinin signaling and plant development
Visit the PubMed ArticleThe arr1-3 arr10-5 arr12-1 mutant exhibited a severely abrogated response to BA, with only one of these 71 genes (ARR5) exhibiting a greater than threefold induction upon treatment with BA
Argyros RD, Mathews DE, Chiang YH, Palmer CM, Thibault DM, Etheridge N, Argyros DA, Mason MG, Kieber JJ, Schaller GE - Type B response regulators of Arabidopsis play key roles in cytokinin signaling and plant development
Visit the PubMed ArticleConsistent with the decreased cell division in the arr1-3 arr10-5 arr12-1 mutant, real-time RT-PCR revealed a significant difference (P < 0.001, t test) in the basal levels of CycD3;1 expression, which is 43% lower in the mutant compared with the wild type
Argyros RD, Mathews DE, Chiang YH, Palmer CM, Thibault DM, Etheridge N, Argyros DA, Mason MG, Kieber JJ, Schaller GE - Type B response regulators of Arabidopsis play key roles in cytokinin signaling and plant development
Visit the PubMed Articlemost suppressed gene in our original analysis of jaw-D ... plants ... TCP4
Schommer C, Palatnik JF, Aggarwal P, Chételat A, Cubas P, Farmer EE, Nath U, Weigel D - Control of jasmonate biosynthesis and senescence by miR319 targets
Visit the PubMed ArticleThe expression of ... LOX4 ... is ... increased in rTCP4 plants
Schommer C, Palatnik JF, Aggarwal P, Chételat A, Cubas P, Farmer EE, Nath U, Weigel D - Control of jasmonate biosynthesis and senescence by miR319 targets
Visit the PubMed Articleother lipoxygenases that are predicted to be chloroplast-localized ... LOX4
Schommer C, Palatnik JF, Aggarwal P, Chételat A, Cubas P, Farmer EE, Nath U, Weigel D - Control of jasmonate biosynthesis and senescence by miR319 targets
Visit the PubMed ArticleThe expression of LOX3 ... is ... increased in rTCP4 plants
Schommer C, Palatnik JF, Aggarwal P, Chételat A, Cubas P, Farmer EE, Nath U, Weigel D - Control of jasmonate biosynthesis and senescence by miR319 targets
Visit the PubMed ArticleLOX2:GUS ... wild-type reporter had strong GUS activity throughout leaves, similar to what has been reported
Schommer C, Palatnik JF, Aggarwal P, Chételat A, Cubas P, Farmer EE, Nath U, Weigel D - Control of jasmonate biosynthesis and senescence by miR319 targets
Visit the PubMed ArticleLOX2 ... chloroplast-localized
Schommer C, Palatnik JF, Aggarwal P, Chételat A, Cubas P, Farmer EE, Nath U, Weigel D - Control of jasmonate biosynthesis and senescence by miR319 targets
Visit the PubMed Articleother lipoxygenases that are predicted to be chloroplast-localized ... LOX3
Schommer C, Palatnik JF, Aggarwal P, Chételat A, Cubas P, Farmer EE, Nath U, Weigel D - Control of jasmonate biosynthesis and senescence by miR319 targets
Visit the PubMed ArticleThe expression of LOX3 ... is reduced in jaw-D plants
Schommer C, Palatnik JF, Aggarwal P, Chételat A, Cubas P, Farmer EE, Nath U, Weigel D - Control of jasmonate biosynthesis and senescence by miR319 targets
Visit the PubMed ArticleLOX2 was the second most suppressed gene in our original analysis of jaw-D plants, after TCP4 itself
Schommer C, Palatnik JF, Aggarwal P, Chételat A, Cubas P, Farmer EE, Nath U, Weigel D - Control of jasmonate biosynthesis and senescence by miR319 targets
Visit the PubMed ArticleLOX2:GUS ... wild-type reporter was less active in tcp2 tcp4 tcp10 triple mutants
Schommer C, Palatnik JF, Aggarwal P, Chételat A, Cubas P, Farmer EE, Nath U, Weigel D - Control of jasmonate biosynthesis and senescence by miR319 targets
Visit the PubMed ArticleWRKY53 ... is induced more than 30 times in rTCP4:GFP plants, although it lacks TCP4 consensus binding motifs in its promoter
Schommer C, Palatnik JF, Aggarwal P, Chételat A, Cubas P, Farmer EE, Nath U, Weigel D - Control of jasmonate biosynthesis and senescence by miR319 targets
Visit the PubMed Articleother lipoxygenases that are predicted to be chloroplast-localized ... LOX6
Schommer C, Palatnik JF, Aggarwal P, Chételat A, Cubas P, Farmer EE, Nath U, Weigel D - Control of jasmonate biosynthesis and senescence by miR319 targets
Visit the PubMed ArticleThe expression of ... LOX4 ... is reduced in jaw-D plants
Schommer C, Palatnik JF, Aggarwal P, Chételat A, Cubas P, Farmer EE, Nath U, Weigel D - Control of jasmonate biosynthesis and senescence by miR319 targets
Visit the PubMed ArticleAtCDT1a ... mRNA levels ... were downregulated in plants with higher levels of ABAP1 mRNA and protein
Masuda HP, Cabral LM, De Veylder L, Tanurdzic M, de Almeida Engler J, Geelen D, Inzé D, Martienssen RA, Ferreira PC, Hemerly AS - ABAP1 is a novel plant Armadillo BTB protein involved in DNA replication and transcription
Visit the PubMed ArticleAtCDT1b mRNA levels ... were downregulated in plants with higher levels of ABAP1 mRNA and protein
Masuda HP, Cabral LM, De Veylder L, Tanurdzic M, de Almeida Engler J, Geelen D, Inzé D, Martienssen RA, Ferreira PC, Hemerly AS - ABAP1 is a novel plant Armadillo BTB protein involved in DNA replication and transcription
Visit the PubMed Articleconfocal and fluorescent microscopy of Arabidopsis plants producing GFP::ABAP1 indicated that ABAP1 was exclusively located in the nucleus, homogeneously distributed or enriched in nuclear domains, and GFP::ABAP1 was absent in mitotic cells
Masuda HP, Cabral LM, De Veylder L, Tanurdzic M, de Almeida Engler J, Geelen D, Inzé D, Martienssen RA, Ferreira PC, Hemerly AS - ABAP1 is a novel plant Armadillo BTB protein involved in DNA replication and transcription
Visit the PubMed ArticleAtCDT1b mRNA levels ... were upregulated in plants where ABAP1 mRNA and protein levels were diminished
Masuda HP, Cabral LM, De Veylder L, Tanurdzic M, de Almeida Engler J, Geelen D, Inzé D, Martienssen RA, Ferreira PC, Hemerly AS - ABAP1 is a novel plant Armadillo BTB protein involved in DNA replication and transcription
Visit the PubMed ArticleGUS expression in the meristemoid and young stomatal cells illustrates ABAP1 expression during stomatal cell differentiation
Masuda HP, Cabral LM, De Veylder L, Tanurdzic M, de Almeida Engler J, Geelen D, Inzé D, Martienssen RA, Ferreira PC, Hemerly AS - ABAP1 is a novel plant Armadillo BTB protein involved in DNA replication and transcription
Visit the PubMed ArticleStudies on localization of ABAP1 expression in plant tissues showed that ABAP1 was ... mainly expressed in ... young leaves ... As the leaf grew, GUS expression decreased in a tip-to-base gradient (Figure 2C and D). In agreement, ABAP1 mRNA levels were higher in developing leaves during proliferation stage (9-day-old plants) and rapidly declined as observed in leaves of 13 till 21-day-old plants
Masuda HP, Cabral LM, De Veylder L, Tanurdzic M, de Almeida Engler J, Geelen D, Inzé D, Martienssen RA, Ferreira PC, Hemerly AS - ABAP1 is a novel plant Armadillo BTB protein involved in DNA replication and transcription
Visit the PubMed ArticleStudies on localization of ABAP1 expression in plant tissues showed that ABAP1 was ... mainly expressed in the shoot apex
Masuda HP, Cabral LM, De Veylder L, Tanurdzic M, de Almeida Engler J, Geelen D, Inzé D, Martienssen RA, Ferreira PC, Hemerly AS - ABAP1 is a novel plant Armadillo BTB protein involved in DNA replication and transcription
Visit the PubMed ArticleAtCDT1a ... mRNA levels ... were upregulated in plants where ABAP1 mRNA and protein levels were diminished
Masuda HP, Cabral LM, De Veylder L, Tanurdzic M, de Almeida Engler J, Geelen D, Inzé D, Martienssen RA, Ferreira PC, Hemerly AS - ABAP1 is a novel plant Armadillo BTB protein involved in DNA replication and transcription
Visit the PubMed ArticleGene expression analyses of 9-day-old AtTCP24OE seedlings with real-time RT–PCR showed that AtCDT1a ... levels were downregulated in AtTCP24OE plants
Masuda HP, Cabral LM, De Veylder L, Tanurdzic M, de Almeida Engler J, Geelen D, Inzé D, Martienssen RA, Ferreira PC, Hemerly AS - ABAP1 is a novel plant Armadillo BTB protein involved in DNA replication and transcription
Visit the PubMed ArticleGene expression analyses of 9-day-old AtTCP24OE seedlings with real-time RT–PCR showed that ... AtCDT1b levels were downregulated in AtTCP24OE plants
Masuda HP, Cabral LM, De Veylder L, Tanurdzic M, de Almeida Engler J, Geelen D, Inzé D, Martienssen RA, Ferreira PC, Hemerly AS - ABAP1 is a novel plant Armadillo BTB protein involved in DNA replication and transcription
Visit the PubMed Articlein plants transformed with 35S:KAN1-GR, a construct that constitutively expresses a dexamethasone (DEX)-inducible form of KAN1. Semiquantitative RT-PCR analysis of 35S:KAN-GR plants grown continuously on DEX or on basal medium followed by DEX ... had no effect on the expression of AS1
Wu G, Lin WC, Huang T, Poethig RS, Springer PS, Kerstetter RA - KANADI1 regulates adaxial-abaxial polarity in Arabidopsis by directly repressing the transcription of ASYMMETRIC LEAVES2
Visit the PubMed ArticlePlants mutant for both KAN1 and KAN2 expressed pAS2:GUS ubiquitously in cotyledons
Wu G, Lin WC, Huang T, Poethig RS, Springer PS, Kerstetter RA - KANADI1 regulates adaxial-abaxial polarity in Arabidopsis by directly repressing the transcription of ASYMMETRIC LEAVES2
Visit the PubMed ArticleIn as2–1, pKAN1:GUS expression extended to the adaxial side of young leaf primordia and persisted late in leaf development
Wu G, Lin WC, Huang T, Poethig RS, Springer PS, Kerstetter RA - KANADI1 regulates adaxial-abaxial polarity in Arabidopsis by directly repressing the transcription of ASYMMETRIC LEAVES2
Visit the PubMed Articlein plants transformed with 35S:KAN1-GR, a construct that constitutively expresses a dexamethasone (DEX)-inducible form of KAN1. Semiquantitative RT-PCR analysis of 35S:KAN-GR plants grown continuously on DEX or on basal medium followed by DEX ... had no effect on the expression of ... FIL1
Wu G, Lin WC, Huang T, Poethig RS, Springer PS, Kerstetter RA - KANADI1 regulates adaxial-abaxial polarity in Arabidopsis by directly repressing the transcription of ASYMMETRIC LEAVES2
Visit the PubMed Articleloss-of-function mutants of KAN1 ... had little effect on the expression of pAS2:GUS in cotyledons (Fig. 5B), but caused this reporter to be expressed on both the adaxial and abaxial sides of leaf primordia
Wu G, Lin WC, Huang T, Poethig RS, Springer PS, Kerstetter RA - KANADI1 regulates adaxial-abaxial polarity in Arabidopsis by directly repressing the transcription of ASYMMETRIC LEAVES2
Visit the PubMed Articleexamined the expression of AS2 and several other polarity genes in plants transformed with 35S:KAN1-GR, a construct that constitutively expresses a dexamethasone (DEX)-inducible form of KAN1. Semiquantitative RT-PCR analysis of 35S:KAN-GR plants grown continuously on DEX or on basal medium followed by DEX ... had no effect on the expression of ... YAB3
Wu G, Lin WC, Huang T, Poethig RS, Springer PS, Kerstetter RA - KANADI1 regulates adaxial-abaxial polarity in Arabidopsis by directly repressing the transcription of ASYMMETRIC LEAVES2
Visit the PubMed ArticleTo examine the effect of the as2–5D mutation on the expression of AS2, we generated promoter–reporter constructs with ... mutated AS2 regulatory sequences fused to ßglucuronidase (GUS). For this purpose we used a 4.1-kb genomic fragment that is capable of rescuing the as2–1 phenotype when fused to an AS2 cDNA [supporting information (SI) Fig. S1]. Constructs with the ... mutated versions of this fragment containing either the as2–5D mutation (pas2–5D:GUS ... were introduced into plants by Agrobacterium-mediated transformation ... plants transformed with pas2–5D:GUS expressed GUS throughout the cotyledons
Wu G, Lin WC, Huang T, Poethig RS, Springer PS, Kerstetter RA - KANADI1 regulates adaxial-abaxial polarity in Arabidopsis by directly repressing the transcription of ASYMMETRIC LEAVES2
Visit the PubMed ArticlePlants mutant for both KAN1 and KAN2 expressed pAS2:GUS ubiquitously in ... leaf primordia
Wu G, Lin WC, Huang T, Poethig RS, Springer PS, Kerstetter RA - KANADI1 regulates adaxial-abaxial polarity in Arabidopsis by directly repressing the transcription of ASYMMETRIC LEAVES2
Visit the PubMed ArticleTo examine the effect of the as2–5D mutation on the expression of AS2, we generated promoter–reporter constructs with ... mutated AS2 regulatory sequences fused to ßglucuronidase (GUS). For this purpose we used a 4.1-kb genomic fragment that is capable of rescuing the as2–1 phenotype when fused to an AS2 cDNA [supporting information (SI) Fig. S1]. Constructs with the ... mutated versions of this fragment containing either the as2–5D mutation (pas2–5D:GUS ... were introduced into plants by Agrobacterium-mediated transformation ... plants transformed with pas2–5D:GUS expressed GUS throughout the cotyledons, leaf primordia, and the hypocotyl
Wu G, Lin WC, Huang T, Poethig RS, Springer PS, Kerstetter RA - KANADI1 regulates adaxial-abaxial polarity in Arabidopsis by directly repressing the transcription of ASYMMETRIC LEAVES2
Visit the PubMed ArticleConsistent with their subtle morphological phenotype, mutations in KAN2 had no apparent effect on pAS2:GUS expression
Wu G, Lin WC, Huang T, Poethig RS, Springer PS, Kerstetter RA - KANADI1 regulates adaxial-abaxial polarity in Arabidopsis by directly repressing the transcription of ASYMMETRIC LEAVES2
Visit the PubMed Articlein as2–5D plants pKAN1:GUS expression was excluded from leaf primordia
Wu G, Lin WC, Huang T, Poethig RS, Springer PS, Kerstetter RA - KANADI1 regulates adaxial-abaxial polarity in Arabidopsis by directly repressing the transcription of ASYMMETRIC LEAVES2
Visit the PubMed Articlein plants transformed with 35S:KAN1-GR, a construct that constitutively expresses a dexamethasone (DEX)-inducible form of KAN1. Semiquantitative RT-PCR analysis of 35S:KAN-GR plants grown continuously on DEX or on basal medium followed by DEX revealed that DEX reduced the amount of AS2 mRNA
Wu G, Lin WC, Huang T, Poethig RS, Springer PS, Kerstetter RA - KANADI1 regulates adaxial-abaxial polarity in Arabidopsis by directly repressing the transcription of ASYMMETRIC LEAVES2
Visit the PubMed Articleusing in situ hybridization to examine the expression of WUS ... Among 14-day-old emb1611-2 seedlings, 50% showed WUS expression in a reduced domain ... in the SAM
Leasure CD, Fiume E, Fletcher JC - The essential gene EMB1611 maintains shoot apical meristem function during Arabidopsis development
Visit the PubMed Articleusing in situ hybridization to ... examine the expression of WUS ... Among 7-day-old emb1611-2 seedlings, 50% had a normal WUS expression domain ... in the SAM
Leasure CD, Fiume E, Fletcher JC - The essential gene EMB1611 maintains shoot apical meristem function during Arabidopsis development
Visit the PubMed Articleusing in situ hybridization to examine the expression of WUS ... Among 7-day-old emb1611-2 seedlings ... 50% displayed no WUS expression in the SAM
Leasure CD, Fiume E, Fletcher JC - The essential gene EMB1611 maintains shoot apical meristem function during Arabidopsis development
Visit the PubMed ArticleSome 13-day-old emb1611-2 seedlings showed a normal pattern of pSTM::uidA activity
Leasure CD, Fiume E, Fletcher JC - The essential gene EMB1611 maintains shoot apical meristem function during Arabidopsis development
Visit the PubMed ArticleThe pattern of pSTM::uidA activity in the periphery of the meristem was largely unaltered in 7-day-old emb1611-2 seedlings ... compared with wild-type seedlings
Leasure CD, Fiume E, Fletcher JC - The essential gene EMB1611 maintains shoot apical meristem function during Arabidopsis development
Visit the PubMed ArticleSome 13-day-old emb1611-2 seedlings ... displayed pSTM::uidA activity throughout the SAM
Leasure CD, Fiume E, Fletcher JC - The essential gene EMB1611 maintains shoot apical meristem function during Arabidopsis development
Visit the PubMed Articleusing in situ hybridization to examine the expression of WUS ... Among 14-day-old emb1611-2 seedlings ... 50% displayed no WUS expression in the SAM
Leasure CD, Fiume E, Fletcher JC - The essential gene EMB1611 maintains shoot apical meristem function during Arabidopsis development
Visit the PubMed Articlepattern of pSTM::uidA activity ... in 7-day-old emb1611-2 seedlings ... a few emb1611-2 individuals showed light GUS staining in the center of the SAM
Leasure CD, Fiume E, Fletcher JC - The essential gene EMB1611 maintains shoot apical meristem function during Arabidopsis development
Visit the PubMed ArticleIn 17-day-old emb1611-2 plants, pSTM::uidA activity was invariably detected throughout the meristem
Leasure CD, Fiume E, Fletcher JC - The essential gene EMB1611 maintains shoot apical meristem function during Arabidopsis development
Visit the PubMed ArticleEMB1611 ... is transcribed at low but equivalent levels in all tissues ... based on RT-PCR
Leasure CD, Fiume E, Fletcher JC - The essential gene EMB1611 maintains shoot apical meristem function during Arabidopsis development
Visit the PubMed Articleusing in situ hybridization to examine the expression of WUS ... Among 14-day-old emb1611-2 seedlings, 50% showed WUS expression ... at lower levels than in wild-type seedlings ... in the SAM
Leasure CD, Fiume E, Fletcher JC - The essential gene EMB1611 maintains shoot apical meristem function during Arabidopsis development
Visit the PubMed ArticleHowever, the expression of TSB1 was not affected in the mutant
Jing Y, Cui D, Bao F, Hu Z, Qin Z, Hu Y - Tryptophan deficiency affects organ growth by retarding cell expansion in Arabidopsis
Visit the PubMed ArticleTo investigate the function of FZR genes in plant development, we obtained two T-DNA insertion mutants for FZR2 from the SALK collection. The fzr2-1 (SALK_083656 ... lines carry insertions in the fourth exon ... Insertions were confirmed by sequencing. RT-PCR analysis showed that both alleles of fzr2 fail to produce full-length transcript
Larson-Rabin Z, Li Z, Masson PH, Day CD - FZR2/CCS52A1 expression is a determinant of endoreduplication and cell expansion in Arabidopsis
Visit the PubMed Articleleaf development by quantitative real-time RT-PCR ... steady expression ... FZR2 ... at 2 to 5 d
Larson-Rabin Z, Li Z, Masson PH, Day CD - FZR2/CCS52A1 expression is a determinant of endoreduplication and cell expansion in Arabidopsis
Visit the PubMed Articleleaf development by quantitative real-time RT-PCR ... steady expression of FZR1 ... at 2 to 5 d
Larson-Rabin Z, Li Z, Masson PH, Day CD - FZR2/CCS52A1 expression is a determinant of endoreduplication and cell expansion in Arabidopsis
Visit the PubMed ArticleFZR2 expression in seedlings
Larson-Rabin Z, Li Z, Masson PH, Day CD - FZR2/CCS52A1 expression is a determinant of endoreduplication and cell expansion in Arabidopsis
Visit the PubMed ArticleFZR2 expression in ... leaves
Larson-Rabin Z, Li Z, Masson PH, Day CD - FZR2/CCS52A1 expression is a determinant of endoreduplication and cell expansion in Arabidopsis
Visit the PubMed ArticleTo investigate the function of FZR genes in plant development, we obtained two T-DNA insertion mutants for FZR2 from the SALK collection ... fzr2-2 (SALK_101689) lines carry insertions in ... the second intron ... Insertions were confirmed by sequencing. RT-PCR analysis showed that both alleles of fzr2 fail to produce full-length transcript
Larson-Rabin Z, Li Z, Masson PH, Day CD - FZR2/CCS52A1 expression is a determinant of endoreduplication and cell expansion in Arabidopsis
Visit the PubMed Articleleaf development by quantitative real-time RT-PCR ... FZR3 was highly expressed in 3- and 5-d-old leaves
Larson-Rabin Z, Li Z, Masson PH, Day CD - FZR2/CCS52A1 expression is a determinant of endoreduplication and cell expansion in Arabidopsis
Visit the PubMed ArticleThe mutant pldε-1 ... resulted in loss of PLDε expression, as indicated by the absence of detectable PLDε transcript
Hong Y, Devaiah SP, Bahn SC, Thamasandra BN, Li M, Welti R, Wang X - Phospholipase D epsilon and phosphatidic acid enhance Arabidopsis nitrogen signaling and growth
Visit the PubMed ArticlePLDε mRNA level was ... low in leaves
Hong Y, Devaiah SP, Bahn SC, Thamasandra BN, Li M, Welti R, Wang X - Phospholipase D epsilon and phosphatidic acid enhance Arabidopsis nitrogen signaling and growth
Visit the PubMed Articlein the AtMRB1 overexpressor plants ... the transcription level of ANT ... significantly upregulated
Guan H, Kang D, Fan M, Chen Z, Qu LJ - Overexpression of a new putative membrane protein gene AtMRB1 results in organ size enlargement in Arabidopsis
Visit the PubMed Articlein the AtMRB1 overexpressor plants ... the transcription level of ... ROT3 ... significantly upregulated
Guan H, Kang D, Fan M, Chen Z, Qu LJ - Overexpression of a new putative membrane protein gene AtMRB1 results in organ size enlargement in Arabidopsis
Visit the PubMed ArticleTo analyze the expression pattern of AtMRB1, we amplified a 1.8 kb long fragment from the AtMRB1 promoter and fused it to β-glucuronidase (GUS) prior to transformation into wild type Arabidopsis. Analysis of the transgenic plants showed that the expression of AtMRB1 was mainly focused in young leaves, hypocotyl, and in the roots of the seedlings
Guan H, Kang D, Fan M, Chen Z, Qu LJ - Overexpression of a new putative membrane protein gene AtMRB1 results in organ size enlargement in Arabidopsis
Visit the PubMed Articlein the AtMRB1 overexpressor plants ... the transcription level of ... BB ... not much altered
Guan H, Kang D, Fan M, Chen Z, Qu LJ - Overexpression of a new putative membrane protein gene AtMRB1 results in organ size enlargement in Arabidopsis
Visit the PubMed ArticleIn adult plants, AtMRB1 transcripts were mainly concentrated in the roots and flower organs, including young upper stems, shoot tips and shoot apical meristems (SAM), and flower buds (Figure 3D–M
Guan H, Kang D, Fan M, Chen Z, Qu LJ - Overexpression of a new putative membrane protein gene AtMRB1 results in organ size enlargement in Arabidopsis
Visit the PubMed ArticleWe transformed wild type Arabidopsis thaliana with the AtMRB1-green fluorescent protein (GFP) fusion protein construct driven by a CaMV 35S promoter and successfully obtained 35 independent transgenic lines used to study the subcellular localization using Confocal Imaging. As shown in Figure 2, green fluorescence was found in the membrane of the cells in undifferentiated cells in the root tip extension zone of AtMRB1-GFP transgenic plants
Guan H, Kang D, Fan M, Chen Z, Qu LJ - Overexpression of a new putative membrane protein gene AtMRB1 results in organ size enlargement in Arabidopsis
Visit the PubMed Articlein the AtMRB1 overexpressor plants ... the transcription level of ... AtGIF ... not much altered
Guan H, Kang D, Fan M, Chen Z, Qu LJ - Overexpression of a new putative membrane protein gene AtMRB1 results in organ size enlargement in Arabidopsis
Visit the PubMed Articlein the AtMRB1 overexpressor plants ... the transcription level of ... GRF5 ... significantly upregulated
Guan H, Kang D, Fan M, Chen Z, Qu LJ - Overexpression of a new putative membrane protein gene AtMRB1 results in organ size enlargement in Arabidopsis
Visit the PubMed ArticleIn adult plants, AtMRB1 transcripts were mainly concentrated in the roots and flower organs, including young upper stems, shoot tips and shoot apical meristems (SAM), and flower buds (Figure 3D–M
Guan H, Kang D, Fan M, Chen Z, Qu LJ - Overexpression of a new putative membrane protein gene AtMRB1 results in organ size enlargement in Arabidopsis
Visit the PubMed ArticleStrong EXO expression was observed in rosette leaves
Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves
Visit the PubMed Articleproteomics approaches also identified the EXO ... as part of the cell wall proteome
Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves
Visit the PubMed ArticleThe EXO:HA protein was detected in the cell wall
Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves
Visit the PubMed Articleexpression was observed in rosette leaves ... EXL5
Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves
Visit the PubMed ArticleSenescing leaves ... displayed little EXO expression
Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves
Visit the PubMed ArticleReal-time RT-PCR analysis demonstrated weaker EXP5 ... expression in the exo mutant in comparison to Col-0 wild-type plants
Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves
Visit the PubMed ArticleReal-time RT-PCR analysis demonstrated weaker ... KCS1 expression in the exo mutant in comparison to Col-0 wild-type plants
Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves
Visit the PubMed Articleexpression was observed in ... cotyledons ... EXL1
Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves
Visit the PubMed Articleproteomics approaches also identified ... EXL2 ... as part of the cell wall proteome
Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves
Visit the PubMed Articleexpression was observed in rosette leaves ... EXL1
Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves
Visit the PubMed Articleexpression was observed in ... cotyledons ... EXL3
Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves
Visit the PubMed Articleproteomics approaches also identified ... EXL3 ... as part of the cell wall proteome
Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves
Visit the PubMed Articleproteomics approaches also identified ... EXL4 ... as part of the cell wall proteome
Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves
Visit the PubMed Articleproteomics approaches also identified ... EXL1 ... as part of the cell wall proteome
Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves
Visit the PubMed ArticleStrong EXO expression was observed in ... cotyledons
Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves
Visit the PubMed ArticleEXL1 ... expression was also induced by BR
Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves
Visit the PubMed Articleexpression was observed in ... cotyledons ... EXL5
Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves
Visit the PubMed Articleexpression was observed in rosette leaves ... EXL3
Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves
Visit the PubMed ArticleEXL5 expression was also induced by BR
Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves
Visit the PubMed Articleexo plants ... WAK1 (Wall-Associated Kinase 1) [22] transcript levels were reduced approximately 2.5-fold in the mutant
Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves
Visit the PubMed Articlea 35S::EXO:GFP construct was stably introduced into Arabidopsis wild-type plants. Green fluorescence was detected in the apoplast. Plasmolysed cells showed fluorescence in the apoplast as well
Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves
Visit the PubMed ArticleEXL3 ... expression was also induced by BR
Schröder F, Lisso J, Lange P, Müssig C - The extracellular EXO protein mediates cell expansion in Arabidopsis leaves
Visit the PubMed ArticleCDKA;1 ... 2690-bp upstream region ... fused it to the β-glucuronidase (GUS) gene ... We then dissected the CDKA;1 promoter and observed GUS expression in tissues. Since Imajuku et al. (2001) have reported that the promoter region from − 1268 bp to the initiation codon was enough to reproduce the expression pattern driven by longer promoter fragments, we generated a deletion series of the 1290-bp region as shown in Fig. 2. The constructs, which were termed 7505, 7506, 7507, 7508, 7509, and 7510, differed with regard to the length of the 5′ region at 100-bp intervals. Seven-day-old seedlings harboring each reporter gene were subjected to GUS staining. We could not find any significant difference in the level and tissue specificity of the expressions of this gene among transgenic lines transformed with the constructs of 7501, 7505, 7506, 7507, 7508, and 7509 (Fig. 3). A shorter incubation in a GUS-staining buffer at a lower temperature (20 °C, 10 min) also showed no significant difference among these lines (Supplemental Fig. 1). However, GUS expression in the 7510 seedlings was lower in both the shoots and roots as compared to that in the above-mentioned lines, although no obvious change was observed in the expression pattern (Fig. 3). We could observe similar quantitative differences in the embryos and inflorescences (Supplemental Fig. 2). These results suggest that the region from − 890 bp to − 791 bp contains cis-regulatory element(s) that promote CDKA;1 expression independently of tissues ... Therefore, we constructed GUS reporter genes with 3′-end truncations of the 1290-bp region. The 7505, 7502R, 7503R, 7504R, 7505R, 7506R, and 7507R constructs differed with regard to the length of the 3′ region at 100-bp intervals (Fig. 2). In the 7-day-old seedlings, we found a significant decrease in the level of GUS expression between the 7502R and 7503R constructs (Fig. 4A). A similar reduction was also noted in embryos (Supplemental Fig. 3), indicating the presence of another cis-regulatory element between − 200 and − 101 bp. In the 7503R–7505R lines, the expression in the roots was gradually reduced as the 3′ end became shorter and, in the roots of the 7506R or 7507R plants, we could not detect any GUS expression (Fig. 4B). Despite the disappearance of GUS expression in roots, we could observe GUS signals in young leaves of the 7506R and 7507R lines (Fig. 4B), indicating the presence of root-specific regulatory elements that promote CDKA;1 expression. Presence of a promoter region enhancing CDKA;1 expression in the leaf epidermis ... 7509F/7R, which carries the region between − 890 bp and − 601 bp (Fig. 2). Although we included the region from − 890 bp to − 791 bp that promotes CDKA;1 expression as mentioned above, the GUS signal was very weak as compared to that in 7507R. However, we could again identify epidermis-specific expression (Fig. 5D), suggesting that this region contains enough information to promote epidermal expression in leaves ... then introduced the 7507R reporter gene into the phbulosa-1d (phb-1d) mutants ... Although the stipules were stained in a similar manner to the wild-type background, the GUS expression in the leaf epidermis was severely suppressed, and almost no expression was detected in any cell type
Adachi S, Nobusawa T, Umeda M - Quantitative and cell type-specific transcriptional regulation of A-type cyclin-dependent kinase in Arabidopsis thaliana
Visit the PubMed ArticleCDKA;1 ... 2690-bp upstream region ... fused it to the β-glucuronidase (GUS) gene ... weakly stained mesophyll cells in the leaves ... We then dissected the CDKA;1 promoter and observed GUS expression in tissues. Since Imajuku et al. (2001) have reported that the promoter region from − 1268 bp to the initiation codon was enough to reproduce the expression pattern driven by longer promoter fragments, we generated a deletion series of the 1290-bp region as shown in Fig. 2. The constructs, which were termed 7505, 7506, 7507, 7508, 7509, and 7510, differed with regard to the length of the 5′ region at 100-bp intervals. Seven-day-old seedlings harboring each reporter gene were subjected to GUS staining. We could not find any significant difference in the level and tissue specificity of the expressions of this gene among transgenic lines transformed with the constructs of 7501, 7505, 7506, 7507, 7508, and 7509 (Fig. 3). A shorter incubation in a GUS-staining buffer at a lower temperature (20 °C, 10 min) also showed no significant difference among these lines (Supplemental Fig. 1). However, GUS expression in the 7510 seedlings was lower in both the shoots and roots as compared to that in the above-mentioned lines, although no obvious change was observed in the expression pattern (Fig. 3). We could observe similar quantitative differences in the embryos and inflorescences (Supplemental Fig. 2). These results suggest that the region from − 890 bp to − 791 bp contains cis-regulatory element(s) that promote CDKA;1 expression independently of tissues ... Therefore, we constructed GUS reporter genes with 3′-end truncations of the 1290-bp region. The 7505, 7502R, 7503R, 7504R, 7505R, 7506R, and 7507R constructs differed with regard to the length of the 3′ region at 100-bp intervals (Fig. 2). In the 7-day-old seedlings, we found a significant decrease in the level of GUS expression between the 7502R and 7503R constructs (Fig. 4A). A similar reduction was also noted in embryos (Supplemental Fig. 3), indicating the presence of another cis-regulatory element between − 200 and − 101 bp. In the 7503R–7505R lines, the expression in the roots was gradually reduced as the 3′ end became shorter and, in the roots of the 7506R or 7507R plants, we could not detect any GUS expression (Fig. 4B). Despite the disappearance of GUS expression in roots, we could observe GUS signals in young leaves of the 7506R and 7507R lines (Fig. 4B), indicating the presence of root-specific regulatory elements that promote CDKA;1 expression. Presence of a promoter region enhancing CDKA;1 expression in the leaf epidermis ... we created another reporter construct, 7509F/7R, which carries the region between − 890 bp and − 601 bp ... we could again identify epidermis-specific expression (Fig. 5D), suggesting that this region contains enough information to promote epidermal expression in leaves
Adachi S, Nobusawa T, Umeda M - Quantitative and cell type-specific transcriptional regulation of A-type cyclin-dependent kinase in Arabidopsis thaliana
Visit the PubMed ArticleWe then dissected the CDKA;1 promoter and observed GUS expression in tissues ... we constructed GUS reporter genes with 3′-end truncations of the 1290-bp region. The 7505, 7502R, 7503R, 7504R, 7505R, 7506R, and 7507R constructs differed with regard to the length of the 3′ region at 100-bp intervals ... We then conducted a detailed analysis of the epidermal expression of this gene by using the 7507R reporter gene. Since the longitudinal sections of shoot apices showed biased GUS staining on the abaxial side of the leaf epidermis (Fig. 5C), we also created transverse sections of the SAM and leaves. As expected, GUS expression was higher on the abaxial side of young leaves, and only a trace level of expression was observed on the adaxial side
Adachi S, Nobusawa T, Umeda M - Quantitative and cell type-specific transcriptional regulation of A-type cyclin-dependent kinase in Arabidopsis thaliana
Visit the PubMed ArticleWe then dissected the CDKA;1 promoter and observed GUS expression in tissues ... To examine cell type-specific regulation of CDKA;1 expression, we made sections of GUS-stained shoot apices ... GUS expression was observed in the ... leaf primordia
Adachi S, Nobusawa T, Umeda M - Quantitative and cell type-specific transcriptional regulation of A-type cyclin-dependent kinase in Arabidopsis thaliana
Visit the PubMed ArticleWe then dissected the CDKA;1 promoter and observed GUS expression in tissues ... we created another reporter construct, 7509F/7R, which carries the region between − 890 bp and − 601 bp (Fig. 2). Although we included the region from − 890 bp to − 791 bp that promotes CDKA;1 expression as mentioned above, the GUS signal was very weak as compared to that in 7507R. However, we could again identify epidermis-specific expression (Fig. 5D), suggesting that this region contains enough information to promote epidermal expression in leaves
Adachi S, Nobusawa T, Umeda M - Quantitative and cell type-specific transcriptional regulation of A-type cyclin-dependent kinase in Arabidopsis thaliana
Visit the PubMed ArticleWe then dissected the CDKA;1 promoter and observed GUS expression in tissues ... To examine cell type-specific regulation of CDKA;1 expression, we made sections of GUS-stained shoot apices ... GUS expression was observed in the ... developing leaves
Adachi S, Nobusawa T, Umeda M - Quantitative and cell type-specific transcriptional regulation of A-type cyclin-dependent kinase in Arabidopsis thaliana
Visit the PubMed ArticleCDKA ... 1 ... 2690-bp upstream region ... fused it to the β-glucuronidase (GUS) gene ... an intense signal in the stomata ... in the leaves ... We then dissected the CDKA;1 promoter and observed GUS expression in tissues. Since Imajuku et al. (2001) have reported that the promoter region from − 1268 bp to the initiation codon was enough to reproduce the expression pattern driven by longer promoter fragments, we generated a deletion series of the 1290-bp region as shown in Fig. 2. The constructs, which were termed 7505, 7506, 7507, 7508, 7509, and 7510, differed with regard to the length of the 5′ region at 100-bp intervals. Seven-day-old seedlings harboring each reporter gene were subjected to GUS staining. We could not find any significant difference in the level and tissue specificity of the expressions of this gene among transgenic lines transformed with the constructs of 7501, 7505, 7506, 7507, 7508, and 7509 (Fig. 3). A shorter incubation in a GUS-staining buffer at a lower temperature (20 °C, 10 min) also showed no significant difference among these lines (Supplemental Fig. 1). However, GUS expression in the 7510 seedlings was lower in both the shoots and roots as compared to that in the above-mentioned lines, although no obvious change was observed in the expression pattern (Fig. 3). We could observe similar quantitative differences in the embryos and inflorescences (Supplemental Fig. 2). These results suggest that the region from − 890 bp to − 791 bp contains cis-regulatory element(s) that promote CDKA;1 expression independently of tissues ... Therefore, we constructed GUS reporter genes with 3′-end truncations of the 1290-bp region. The 7505, 7502R, 7503R, 7504R, 7505R, 7506R, and 7507R constructs differed with regard to the length of the 3′ region at 100-bp intervals (Fig. 2). In the 7-day-old seedlings, we found a significant decrease in the level of GUS expression between the 7502R and 7503R constructs (Fig. 4A). A similar reduction was also noted in embryos (Supplemental Fig. 3), indicating the presence of another cis-regulatory element between − 200 and − 101 bp. In the 7503R–7505R lines, the expression in the roots was gradually reduced as the 3′ end became shorter and, in the roots of the 7506R or 7507R plants, we could not detect any GUS expression (Fig. 4B). Despite the disappearance of GUS expression in roots, we could observe GUS signals in young leaves of the 7506R and 7507R lines (Fig. 4B), indicating the presence of root-specific regulatory elements that promote CDKA;1 expression. Presence of a promoter region enhancing CDKA;1 expression in the leaf epidermis ... we created another reporter construct, 7509F/7R, which carries the region between − 890 bp and − 601 bp ... we could again identify epidermis-specific expression (Fig. 5D), suggesting that this region contains enough information to promote epidermal expression in leaves
Adachi S, Nobusawa T, Umeda M - Quantitative and cell type-specific transcriptional regulation of A-type cyclin-dependent kinase in Arabidopsis thaliana
Visit the PubMed ArticleWe then dissected the CDKA;1 promoter and observed GUS expression in tissues ... 7503R exhibited lower GUS expression in the SAM and no expression in the inner layers of leaves. The results showed that the epidermis of leaves and trichomes were prominently GUS-stained (Fig. 5C). This indicates that the region between − 200 bp and − 101 bp is associated with CDKA;1 expression in the ... leaves except for the epidermis ... The epidermis-specific expression pattern was also observed in 7504R–7507R ... In situ hybridization using probes for transcripts of GUS showed the similar epidermis-specific expression in leaves of the 7503R and 7507R lines
Adachi S, Nobusawa T, Umeda M - Quantitative and cell type-specific transcriptional regulation of A-type cyclin-dependent kinase in Arabidopsis thaliana
Visit the PubMed ArticleWe then dissected the CDKA;1 promoter and observed GUS expression in tissues ... we constructed GUS reporter genes with 3′-end truncations of the 1290-bp region. The 7505, 7502R, 7503R, 7504R, 7505R, 7506R, and 7507R constructs differed with regard to the length of the 3′ region at 100-bp intervals ... 7503R exhibited lower GUS expression in the SAM and no expression in the inner layers of leaves. The results showed that the epidermis of leaves and trichomes were prominently GUS-stained (Fig. 5C). This indicates that the region between − 200 bp and − 101 bp is associated with CDKA;1 expression in the ... leaves except for the epidermis ... The epidermis-specific expression pattern was also observed in 7504R–7507R
Adachi S, Nobusawa T, Umeda M - Quantitative and cell type-specific transcriptional regulation of A-type cyclin-dependent kinase in Arabidopsis thaliana
Visit the PubMed ArticleWe then dissected the CDKA;1 promoter and observed GUS expression in tissues ... we constructed GUS reporter genes with 3′-end truncations of the 1290-bp region. The 7505, 7502R, 7503R, 7504R, 7505R, 7506R, and 7507R constructs differed with regard to the length of the 3′ region at 100-bp intervals ... We then conducted a detailed analysis of the epidermal expression of this gene by using the 7507R reporter gene. Since the longitudinal sections of shoot apices showed biased GUS staining on the abaxial side of the leaf epidermis (Fig. 5C), we also created transverse sections of the SAM and leaves. As expected, GUS expression was higher on the abaxial side of young leaves, and only a trace level of expression was observed on the adaxial side
Adachi S, Nobusawa T, Umeda M - Quantitative and cell type-specific transcriptional regulation of A-type cyclin-dependent kinase in Arabidopsis thaliana
Visit the PubMed ArticleWe then dissected the CDKA;1 promoter and observed GUS expression in tissues ... 7503R exhibited lower GUS expression in the SAM and no expression in the inner layers of leaves. The results showed that the epidermis of leaves and trichomes were prominently GUS-stained (Fig. 5C). This indicates that the region between − 200 bp and − 101 bp is associated with CDKA;1 expression in the ... leaves except for the epidermis
Adachi S, Nobusawa T, Umeda M - Quantitative and cell type-specific transcriptional regulation of A-type cyclin-dependent kinase in Arabidopsis thaliana
Visit the PubMed ArticleMED21 ... Significant expression in rosette leaves
Dhawan R, Luo H, Foerster AM, Abuqamar S, Du HN, Briggs SD, Mittelsten Scheid O, Mengiste T - HISTONE MONOUBIQUITINATION1 interacts with a subunit of the mediator complex and regulates defense against necrotrophic fungal pathogens in Arabidopsis
Visit the PubMed ArticleHERK1 ... expression levels ... were ... increased in the constitutive BR-response mutant bes1-D
Guo H, Li L, Ye H, Yu X, Algreen A, Yin Y - Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana
Visit the PubMed ArticleTHE1 ... localized to the plasma membrane
Guo H, Li L, Ye H, Yu X, Algreen A, Yin Y - Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana
Visit the PubMed ArticleFER ... universally expressed in most vegetative tissues, including leaves, stems and roots as revealed by our promoter-GUS (β-glucuronidase) reporter gene studies
Guo H, Li L, Ye H, Yu X, Algreen A, Yin Y - Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana
Visit the PubMed ArticleHERK1 ... expression is particularly strong in the regions undergoing cell elongation, such as in hypocotyls and leaf petioles
Guo H, Li L, Ye H, Yu X, Algreen A, Yin Y - Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana
Visit the PubMed ArticleIn our effort to identify and characterize BR regulated genes, we found that HERK1, THE1 and FER are induced by BRs as revealed by both published (15) and publicly available microarray data (http://bbc.botany.utoronto.ca/efp/cgi-bin/efpWeb.cgi). HERK1, THE1, and FER are the most highly induced genes by BR and, therefore, were chosen for further study. Our quantitative RT-PCR experiments confirmed that brassinolide (BL), the most active BR, up-regulated these RLKs by ≈20–80% in seedlings and/or adult plants (Fig. 1A
Guo H, Li L, Ye H, Yu X, Algreen A, Yin Y - Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana
Visit the PubMed ArticleIn our effort to identify and characterize BR regulated genes, we found that HERK1, THE1 and FER are induced by BRs as revealed by both published (15) and publicly available microarray data (http://bbc.botany.utoronto.ca/efp/cgi-bin/efpWeb.cgi). HERK1, THE1, and FER are the most highly induced genes by BR and, therefore, were chosen for further study. Our quantitative RT-PCR experiments confirmed that brassinolide (BL), the most active BR, up-regulated these RLKs by ≈20–80% in seedlings and/or adult plants (Fig. 1A
Guo H, Li L, Ye H, Yu X, Algreen A, Yin Y - Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana
Visit the PubMed ArticleFER ... localized to the plasma membrane
Guo H, Li L, Ye H, Yu X, Algreen A, Yin Y - Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana
Visit the PubMed ArticleFER ... expression levels ... were reduced in the loss-of-function mutant bri1
Guo H, Li L, Ye H, Yu X, Algreen A, Yin Y - Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana
Visit the PubMed ArticleFER ... expression is particularly strong in the regions undergoing cell elongation, such as in hypocotyls and leaf petioles
Guo H, Li L, Ye H, Yu X, Algreen A, Yin Y - Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana
Visit the PubMed ArticleHERK1-GFP fusion localized to the plasma membrane when expressed in Arabidopsis protoplasts and plants
Guo H, Li L, Ye H, Yu X, Algreen A, Yin Y - Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana
Visit the PubMed ArticleTHE1 ... universally expressed in most vegetative tissues, including leaves, stems and roots as revealed by our promoter-GUS (β-glucuronidase) reporter gene studies
Guo H, Li L, Ye H, Yu X, Algreen A, Yin Y - Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana
Visit the PubMed ArticleHERK1 ... expression levels ... were reduced in the loss-of-function mutant bri1
Guo H, Li L, Ye H, Yu X, Algreen A, Yin Y - Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana
Visit the PubMed ArticleHERK1 ... universally expressed in most vegetative tissues, including leaves, stems and roots as revealed by our promoter-GUS (β-glucuronidase) reporter gene studies
Guo H, Li L, Ye H, Yu X, Algreen A, Yin Y - Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana
Visit the PubMed ArticleTHE1 ... expression levels ... were ... increased in the constitutive BR-response mutant bes1-D
Guo H, Li L, Ye H, Yu X, Algreen A, Yin Y - Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana
Visit the PubMed ArticleTHE1 ... expression levels ... were reduced in the loss-of-function mutant bri1
Guo H, Li L, Ye H, Yu X, Algreen A, Yin Y - Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana
Visit the PubMed ArticleIn our effort to identify and characterize BR regulated genes, we found that HERK1, THE1 and FER are induced by BRs as revealed by both published (15) and publicly available microarray data (http://bbc.botany.utoronto.ca/efp/cgi-bin/efpWeb.cgi). HERK1, THE1, and FER are the most highly induced genes by BR and, therefore, were chosen for further study. Our quantitative RT-PCR experiments confirmed that brassinolide (BL), the most active BR, up-regulated these RLKs by ≈20–80% in seedlings and/or adult plants (Fig. 1A
Guo H, Li L, Ye H, Yu X, Algreen A, Yin Y - Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana
Visit the PubMed ArticleFER ... expression levels ... were ... increased in the constitutive BR-response mutant bes1-D
Guo H, Li L, Ye H, Yu X, Algreen A, Yin Y - Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana
Visit the PubMed ArticleTHE1 ... expression is particularly strong in the regions undergoing cell elongation, such as in hypocotyls and leaf petioles
Guo H, Li L, Ye H, Yu X, Algreen A, Yin Y - Three related receptor-like kinases are required for optimal cell elongation in Arabidopsis thaliana
Visit the PubMed ArticleThe expression pattern of SPI was determined by RT-PCR analysis. SPI could be amplified from total RNA of all organs tested, including the flowers, cauline leaves, stem, rosette leaves, hypocotyl and roots
Saedler R, Jakoby M, Marin B, Galiana-Jaime E, Hülskamp M - The cell morphogenesis gene SPIRRIG in Arabidopsis encodes a WD/BEACH domain protein
Visit the PubMed ArticleWhen we examined the accumula- tion of AN3 transcript in the ... oli5 ... by RT-PCR analysis, it accumulated at a comparable level to that in wild-type
Fujikura U, Horiguchi G, Ponce MR, Micol JL, Tsukaya H - Coordination of cell proliferation and cell expansion mediated by ribosome-related processes in the leaves of Arabidopsis thaliana
Visit the PubMed ArticleWhen we examined the accumula- tion of AN3 transcript in the ... oli7 ... by RT-PCR analysis, it accumulated at a comparable level to that in wild-type
Fujikura U, Horiguchi G, Ponce MR, Micol JL, Tsukaya H - Coordination of cell proliferation and cell expansion mediated by ribosome-related processes in the leaves of Arabidopsis thaliana
Visit the PubMed ArticleWhen we examined the accumula- tion of AN3 transcript in the oli2 ... by RT-PCR analysis, it accumulated at a comparable level to that in wild-type
Fujikura U, Horiguchi G, Ponce MR, Micol JL, Tsukaya H - Coordination of cell proliferation and cell expansion mediated by ribosome-related processes in the leaves of Arabidopsis thaliana
Visit the PubMed ArticleThe bil1bil2 mutation increases the SAUR-ACS1 transcript level by approximately 2-fold
Yan Z, Zhao J, Peng P, Chihara RK, Li J - BIN2 functions redundantly with other Arabidopsis GSK3-like kinases to regulate brassinosteroid signaling
Visit the PubMed Articlewithout BR treatment, bin2-3 mutants accumulated more SAUR-AC1 transcripts ... than wild-type plants
Yan Z, Zhao J, Peng P, Chihara RK, Li J - BIN2 functions redundantly with other Arabidopsis GSK3-like kinases to regulate brassinosteroid signaling
Visit the PubMed Articlewithout BR treatment, bin2-3 mutants accumulate an easily detectable amount of hypophosphorylated BES1 compared with wild-type control plants grown under the same growth conditions
Yan Z, Zhao J, Peng P, Chihara RK, Li J - BIN2 functions redundantly with other Arabidopsis GSK3-like kinases to regulate brassinosteroid signaling
Visit the PubMed Article1 h of BL treatment stimulated the expression of SAUR-AC1
Yan Z, Zhao J, Peng P, Chihara RK, Li J - BIN2 functions redundantly with other Arabidopsis GSK3-like kinases to regulate brassinosteroid signaling
Visit the PubMed Article1 h of BL treatment ... inhibited the accumulation of transcripts of ... CPD
Yan Z, Zhao J, Peng P, Chihara RK, Li J - BIN2 functions redundantly with other Arabidopsis GSK3-like kinases to regulate brassinosteroid signaling
Visit the PubMed Article1 h of BL treatment ... inhibited the accumulation of transcripts of ... DWF4
Yan Z, Zhao J, Peng P, Chihara RK, Li J - BIN2 functions redundantly with other Arabidopsis GSK3-like kinases to regulate brassinosteroid signaling
Visit the PubMed ArticleSAUR-AC1 expression was significantly enhanced in the bin2-3bil1bil3 triple mutant, with a nearly 7-fold increase compared with an approximately 3-fold BR-elicited induction in wild-type seedlings
Yan Z, Zhao J, Peng P, Chihara RK, Li J - BIN2 functions redundantly with other Arabidopsis GSK3-like kinases to regulate brassinosteroid signaling
Visit the PubMed Articlewithout BR treatment, bin2-3 mutants accumulated ... lower levels of DWF4 ... mRNAs than wild-type plants
Yan Z, Zhao J, Peng P, Chihara RK, Li J - BIN2 functions redundantly with other Arabidopsis GSK3-like kinases to regulate brassinosteroid signaling
Visit the PubMed Articlewithout BR treatment, bin2-3 mutants accumulated ... lower levels of ... CPD mRNAs than wild-type plants
Yan Z, Zhao J, Peng P, Chihara RK, Li J - BIN2 functions redundantly with other Arabidopsis GSK3-like kinases to regulate brassinosteroid signaling
Visit the PubMed Articlebin2-3bil1bil2 ... the transcript level of CPD was reduced by approximately 80% in the triple mutant compared with an approximately 40% reduction in BR-treated wild-type plants
Yan Z, Zhao J, Peng P, Chihara RK, Li J - BIN2 functions redundantly with other Arabidopsis GSK3-like kinases to regulate brassinosteroid signaling
Visit the PubMed Articlewild-type plants predominantly accumulate the hyperphosphorylated form of BES1, and BL treatment results in rapid disappearance of the hyperphosphorylated BES1 accompanied by accumulation of hypophosphorylated BES1, which has a faster electrophoretic mobility on western blot
Yan Z, Zhao J, Peng P, Chihara RK, Li J - BIN2 functions redundantly with other Arabidopsis GSK3-like kinases to regulate brassinosteroid signaling
Visit the PubMed ArticleThe bil1bil2 mutation ... reduces the CPD mRNA level by approximately 30%
Yan Z, Zhao J, Peng P, Chihara RK, Li J - BIN2 functions redundantly with other Arabidopsis GSK3-like kinases to regulate brassinosteroid signaling
Visit the PubMed ArticlepEPF2:GUS expressed ... in very young leaves and during leaf development became restricted to meristemoids and GMCs
Hunt L, Gray JE - The signaling peptide EPF2 controls asymmetric cell divisions during stomatal development
Visit the PubMed ArticleIn transgenic plants harboring EPF1–GFP ... EPF1 ... activated green fluorescent protein ... in stomatal precursors
Hara K, Yokoo T, Kajita R, Onishi T, Yahata S, Peterson KM, Torii KU, Kakimoto T - Epidermal cell density is autoregulated via a secretory peptide, EPIDERMAL PATTERNING FACTOR 2 in Arabidopsis leaves
Visit the PubMed ArticleAn in situ RNA hybridization experiment showed that the EPF2 message ... was also expressed in another type of cells with a small quadrangular shape, which are perhaps cells with asymmetric cell division competency, including MMCs
Hara K, Yokoo T, Kajita R, Onishi T, Yahata S, Peterson KM, Torii KU, Kakimoto T - Epidermal cell density is autoregulated via a secretory peptide, EPIDERMAL PATTERNING FACTOR 2 in Arabidopsis leaves
Visit the PubMed ArticleOverexpression of ... EPF2 decreased the number of cells expressing EPF1–GFP
Hara K, Yokoo T, Kajita R, Onishi T, Yahata S, Peterson KM, Torii KU, Kakimoto T - Epidermal cell density is autoregulated via a secretory peptide, EPIDERMAL PATTERNING FACTOR 2 in Arabidopsis leaves
Visit the PubMed ArticleEPF2–GFP was not detected in guard cells nor in any non-epidermal cells
Hara K, Yokoo T, Kajita R, Onishi T, Yahata S, Peterson KM, Torii KU, Kakimoto T - Epidermal cell density is autoregulated via a secretory peptide, EPIDERMAL PATTERNING FACTOR 2 in Arabidopsis leaves
Visit the PubMed ArticleIn ... transgenic plants harboring ... EPF2–GFP ... At1g34245 ... activated green fluorescent protein ... in stomatal precursors
Hara K, Yokoo T, Kajita R, Onishi T, Yahata S, Peterson KM, Torii KU, Kakimoto T - Epidermal cell density is autoregulated via a secretory peptide, EPIDERMAL PATTERNING FACTOR 2 in Arabidopsis leaves
Visit the PubMed ArticleEPF1–GFP ... were expressed ... not in the spch homozygotes
Hara K, Yokoo T, Kajita R, Onishi T, Yahata S, Peterson KM, Torii KU, Kakimoto T - Epidermal cell density is autoregulated via a secretory peptide, EPIDERMAL PATTERNING FACTOR 2 in Arabidopsis leaves
Visit the PubMed ArticleOverexpression of neither EPF1 ... affected TMM–GFP expression
Hara K, Yokoo T, Kajita R, Onishi T, Yahata S, Peterson KM, Torii KU, Kakimoto T - Epidermal cell density is autoregulated via a secretory peptide, EPIDERMAL PATTERNING FACTOR 2 in Arabidopsis leaves
Visit the PubMed ArticleReverse transcription–PCR (RT–PCR) analysis revealed that EPF2 ... is preferentially expressed in the aerial organs
Hara K, Yokoo T, Kajita R, Onishi T, Yahata S, Peterson KM, Torii KU, Kakimoto T - Epidermal cell density is autoregulated via a secretory peptide, EPIDERMAL PATTERNING FACTOR 2 in Arabidopsis leaves
Visit the PubMed ArticleOverexpression of neither ... EPF2 affected TMM–GFP expression
Hara K, Yokoo T, Kajita R, Onishi T, Yahata S, Peterson KM, Torii KU, Kakimoto T - Epidermal cell density is autoregulated via a secretory peptide, EPIDERMAL PATTERNING FACTOR 2 in Arabidopsis leaves
Visit the PubMed ArticleOverexpression of ... EPF1 ... decreased the number of cells expressing EPF1–GFP
Hara K, Yokoo T, Kajita R, Onishi T, Yahata S, Peterson KM, Torii KU, Kakimoto T - Epidermal cell density is autoregulated via a secretory peptide, EPIDERMAL PATTERNING FACTOR 2 in Arabidopsis leaves
Visit the PubMed ArticleAn in situ RNA hybridization experiment showed that ... During early leaf development, the onset of the EPF2–GFP signal preceded the emergence of meristemoids ... signal was later detected in meristemoids, their sister cells and GMCs
Hara K, Yokoo T, Kajita R, Onishi T, Yahata S, Peterson KM, Torii KU, Kakimoto T - Epidermal cell density is autoregulated via a secretory peptide, EPIDERMAL PATTERNING FACTOR 2 in Arabidopsis leaves
Visit the PubMed ArticleReverse transcription–PCR (RT–PCR) analysis revealed that ... EPF1 ... is preferentially expressed in the aerial organs
Hara K, Yokoo T, Kajita R, Onishi T, Yahata S, Peterson KM, Torii KU, Kakimoto T - Epidermal cell density is autoregulated via a secretory peptide, EPIDERMAL PATTERNING FACTOR 2 in Arabidopsis leaves
Visit the PubMed ArticleEPF2–GFP ... were expressed ... not in the spch homozygotes
Hara K, Yokoo T, Kajita R, Onishi T, Yahata S, Peterson KM, Torii KU, Kakimoto T - Epidermal cell density is autoregulated via a secretory peptide, EPIDERMAL PATTERNING FACTOR 2 in Arabidopsis leaves
Visit the PubMed Articleoverexpression of EPF1 ... did not reduce the number of cells expressing EPF2–GFP
Hara K, Yokoo T, Kajita R, Onishi T, Yahata S, Peterson KM, Torii KU, Kakimoto T - Epidermal cell density is autoregulated via a secretory peptide, EPIDERMAL PATTERNING FACTOR 2 in Arabidopsis leaves
Visit the PubMed ArticleEPF2-overexpressors had a decreased number of cells that express EPF2–GFP
Hara K, Yokoo T, Kajita R, Onishi T, Yahata S, Peterson KM, Torii KU, Kakimoto T - Epidermal cell density is autoregulated via a secretory peptide, EPIDERMAL PATTERNING FACTOR 2 in Arabidopsis leaves
Visit the PubMed ArticleTMM–GFP ... were expressed ... not in the spch homozygotes
Hara K, Yokoo T, Kajita R, Onishi T, Yahata S, Peterson KM, Torii KU, Kakimoto T - Epidermal cell density is autoregulated via a secretory peptide, EPIDERMAL PATTERNING FACTOR 2 in Arabidopsis leaves
Visit the PubMed Articlein 35S:MIR396 transgenic plants ... levels of ... AtGRF3 transcripts were ... lower than those in control plants
Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis
Visit the PubMed ArticleAtGRF8 ... mRNA levels were reduced in transgenic 35S:MIR396 plant leaves relative to those in control plants
Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis
Visit the PubMed ArticleAtGRF4 mRNA levels were also reduced in transgenic 35S:MIR396 plant leaves compared with control plants
Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis
Visit the PubMed ArticleAtGRF7 ... mRNA levels were reduced in transgenic 35S:MIR396 plant leaves relative to those in control plants
Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis
Visit the PubMed ArticleBoth RNA gel blot analysis and RT-PCR analysis showed that the expression levels of AN3 were significantly reduced in the transgenic 35S:MIR396a ... plants
Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis
Visit the PubMed ArticleWild-type plants (Col-0) (3 weeks old) grown in soil were subjected to drought stress by withholding watering and the levels of miR396 were determined by RNA blot analysis. As shown in Fig. 11, the level of miR396 was slightly increased in 1 day of watering withholding and was further elevated 2 days later. The levels of miR396 remained substantially higher after 4 and 5 days of drought stress than those detected prior to drought stress treatment. These results indicated that the level of miR396 was induced by drought stress
Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis
Visit the PubMed ArticleAtGRF8 ... mRNA levels were reduced in transgenic 35S:MIR396 plant leaves relative to those in control plants
Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis
Visit the PubMed Articlein 35S:MIR396 transgenic plants ... levels of ... AtGRF3 transcripts were ... lower than those in control plants
Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis
Visit the PubMed Articlein 35S:MIR396 transgenic plants ... levels of AtGRF1 ... transcripts were ... lower than those in control plants
Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis
Visit the PubMed Articlein 35S:MIR396 transgenic plants ... levels of AtGRF1 ... transcripts were ... lower than those in control plants
Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis
Visit the PubMed Articlethe transcript levels for ... AtGRF3 ... were significantly lower after 4 days of watering withholding than those detected at the beginning of drought stress
Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis
Visit the PubMed ArticleAtGRF9 mRNA levels were reduced in transgenic 35S:MIR396 plant leaves relative to those in control plants
Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis
Visit the PubMed Articlethe transcript levels for ... AN3 were significantly lower after 4 days of watering withholding than those detected at the beginning of drought stress
Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis
Visit the PubMed Articlein 35S:MIR396 transgenic plants ... levels of ... AtGRF2 ... transcripts were ... lower than those in control plants
Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis
Visit the PubMed Articlein 35S:MIR396 transgenic plants ... levels of ... AtGRF2 ... transcripts were ... lower than those in control plants
Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis
Visit the PubMed ArticleAtGRF4 mRNA levels were also reduced in transgenic 35S:MIR396 plant leaves compared with control plants
Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis
Visit the PubMed ArticleAtGRF9 mRNA levels were reduced in transgenic 35S:MIR396 plant leaves relative to those in control plants
Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis
Visit the PubMed ArticleAtGRF7 ... mRNA levels were reduced in transgenic 35S:MIR396 plant leaves relative to those in control plants
Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis
Visit the PubMed ArticlemiR396 was broadly an expression profile and strongly expressed in 6-day-old seedlings and rosette leaves
Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis
Visit the PubMed Articlethe transcript levels for AtGRF1 ... were significantly lower after 4 days of watering withholding than those detected at the beginning of drought stress
Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis
Visit the PubMed ArticleBoth RNA gel blot analysis and RT-PCR analysis showed that the expression levels of AN3 were significantly reduced in the transgenic ... 35S:MIR396b plants
Liu D, Song Y, Chen Z, Yu D - Ectopic expression of miR396 suppresses GRF target gene expression and alters leaf growth in Arabidopsis
Visit the PubMed ArticleTranscripts for AtRPT2a ... were detectable in all organs tested at both 16 and 45 days after sowing (DAS)
Sonoda Y, Sako K, Maki Y, Yamazaki N, Yamamoto H, Ikeda A, Yamaguchi J - Regulation of leaf organ size by the Arabidopsis RPT2a 19S proteasome subunit
Visit the PubMed ArticleAt the proliferation stage (11 DAS), there was no difference in KRP2 transcript accumulation between the wild type and rpt2a-2
Sonoda Y, Sako K, Maki Y, Yamazaki N, Yamamoto H, Ikeda A, Yamaguchi J - Regulation of leaf organ size by the Arabidopsis RPT2a 19S proteasome subunit
Visit the PubMed ArticleTranscripts for ... AtRPT2b were detectable in all organs tested at both 16 and 45 days after sowing (DAS)
Sonoda Y, Sako K, Maki Y, Yamazaki N, Yamamoto H, Ikeda A, Yamaguchi J - Regulation of leaf organ size by the Arabidopsis RPT2a 19S proteasome subunit
Visit the PubMed ArticleAtRPT2a ... was detectable in ... trichomes
Sonoda Y, Sako K, Maki Y, Yamazaki N, Yamamoto H, Ikeda A, Yamaguchi J - Regulation of leaf organ size by the Arabidopsis RPT2a 19S proteasome subunit
Visit the PubMed ArticleAt the proliferation stage (11 DAS) ... the expression of ... CYCB1;1 ... was increased in rpt2a-2 compared with the wild type
Sonoda Y, Sako K, Maki Y, Yamazaki N, Yamamoto H, Ikeda A, Yamaguchi J - Regulation of leaf organ size by the Arabidopsis RPT2a 19S proteasome subunit
Visit the PubMed ArticleThe AtRPT2a promoter showed strong activity in the shoot meristem
Sonoda Y, Sako K, Maki Y, Yamazaki N, Yamamoto H, Ikeda A, Yamaguchi J - Regulation of leaf organ size by the Arabidopsis RPT2a 19S proteasome subunit
Visit the PubMed ArticleAtRPT2a ... was detectable in various tissues, such as expanded cotyledons, vascular cells and trichomes
Sonoda Y, Sako K, Maki Y, Yamazaki N, Yamamoto H, Ikeda A, Yamaguchi J - Regulation of leaf organ size by the Arabidopsis RPT2a 19S proteasome subunit
Visit the PubMed ArticleAtRPT2b ... was detectable in ... trichomes
Sonoda Y, Sako K, Maki Y, Yamazaki N, Yamamoto H, Ikeda A, Yamaguchi J - Regulation of leaf organ size by the Arabidopsis RPT2a 19S proteasome subunit
Visit the PubMed ArticleAtRPT2b ... was detectable in various tissues, such as expanded cotyledons, vascular cells and trichomes
Sonoda Y, Sako K, Maki Y, Yamazaki N, Yamamoto H, Ikeda A, Yamaguchi J - Regulation of leaf organ size by the Arabidopsis RPT2a 19S proteasome subunit
Visit the PubMed ArticleAt the proliferation stage (11 DAS) ... the expression of ... KRP1 was increased in rpt2a-2 compared with the wild type
Sonoda Y, Sako K, Maki Y, Yamazaki N, Yamamoto H, Ikeda A, Yamaguchi J - Regulation of leaf organ size by the Arabidopsis RPT2a 19S proteasome subunit
Visit the PubMed ArticleAt the proliferation stage (11 DAS) ... the expression of CDKB1;1 ... was increased in rpt2a-2 compared with the wild type
Sonoda Y, Sako K, Maki Y, Yamazaki N, Yamamoto H, Ikeda A, Yamaguchi J - Regulation of leaf organ size by the Arabidopsis RPT2a 19S proteasome subunit
Visit the PubMed ArticleAtRPT2b showed strong activity in both the shoot and root meristems
Sonoda Y, Sako K, Maki Y, Yamazaki N, Yamamoto H, Ikeda A, Yamaguchi J - Regulation of leaf organ size by the Arabidopsis RPT2a 19S proteasome subunit
Visit the PubMed ArticleAt4g37740/GRF2 ... was also increased in the gra-D mutants
Horiguchi G, Gonzalez N, Beemster GT, Inzé D, Tsukaya H - Impact of segmental chromosomal duplications on leaf size in the grandifolia-D mutants of Arabidopsis thaliana
Visit the PubMed Articlewe further analyzed the expression array data. In the commonly duplicated regions, we found four cyclin genes, CYCD3;1 (At4g34160), CYCB1;1 (At4g37490), CYCB2;2 (At4g35620) and CYCD5;1 (At4g37630) (Vandepoele et al., 2002) ... The expression of these genes in at least two gra-D mutants was more than 1.36-fold higher than that in wild-type (P < 0.05, Table S1)
Horiguchi G, Gonzalez N, Beemster GT, Inzé D, Tsukaya H - Impact of segmental chromosomal duplications on leaf size in the grandifolia-D mutants of Arabidopsis thaliana
Visit the PubMed Articlea bHLH transcription factor BEE2 (At4g36540) (Friedrichsen et al., 2002) that positively controls brassinosteroid signaling, showed elevated expression levels in all three gra-D mutants (P < 0.05, Table S1)
Horiguchi G, Gonzalez N, Beemster GT, Inzé D, Tsukaya H - Impact of segmental chromosomal duplications on leaf size in the grandifolia-D mutants of Arabidopsis thaliana
Visit the PubMed ArticleANT ... expression ... in at least two gra-D mutants was more than 1.36-fold higher than that in wild-type (P < 0.05, Table S1
Horiguchi G, Gonzalez N, Beemster GT, Inzé D, Tsukaya H - Impact of segmental chromosomal duplications on leaf size in the grandifolia-D mutants of Arabidopsis thaliana
Visit the PubMed Articlein the gra-D mutants ... the expression of ... TUB4 on chromosome 5 were normal
Horiguchi G, Gonzalez N, Beemster GT, Inzé D, Tsukaya H - Impact of segmental chromosomal duplications on leaf size in the grandifolia-D mutants of Arabidopsis thaliana
Visit the PubMed ArticleSMP2 ... expression ... in at least two gra-D mutants was more than 1.36-fold higher than that in wild-type (P < 0.05, Table S1
Horiguchi G, Gonzalez N, Beemster GT, Inzé D, Tsukaya H - Impact of segmental chromosomal duplications on leaf size in the grandifolia-D mutants of Arabidopsis thaliana
Visit the PubMed Articlewe further analyzed the expression array data. In the commonly duplicated regions, we found four cyclin genes, CYCD3;1 (At4g34160), CYCB1;1 (At4g37490), CYCB2;2 (At4g35620) and CYCD5;1 (At4g37630) (Vandepoele et al., 2002) ... The expression of these genes in at least two gra-D mutants was more than 1.36-fold higher than that in wild-type (P < 0.05, Table S1)
Horiguchi G, Gonzalez N, Beemster GT, Inzé D, Tsukaya H - Impact of segmental chromosomal duplications on leaf size in the grandifolia-D mutants of Arabidopsis thaliana
Visit the PubMed Articlea brassinosteroid-signaling kinase BSK1 (At4g35230) (Tang et al., 2008) ... showed elevated expression levels in all three gra-D mutants (P < 0.05, Table S1)
Horiguchi G, Gonzalez N, Beemster GT, Inzé D, Tsukaya H - Impact of segmental chromosomal duplications on leaf size in the grandifolia-D mutants of Arabidopsis thaliana
Visit the PubMed Articlewe further analyzed the expression array data. In the commonly duplicated regions, we found four cyclin genes, CYCD3;1 (At4g34160), CYCB1;1 (At4g37490), CYCB2;2 (At4g35620) and CYCD5;1 (At4g37630) (Vandepoele et al., 2002) ... The expression of these genes in at least two gra-D mutants was more than 1.36-fold higher than that in wild-type (P < 0.05, Table S1)
Horiguchi G, Gonzalez N, Beemster GT, Inzé D, Tsukaya H - Impact of segmental chromosomal duplications on leaf size in the grandifolia-D mutants of Arabidopsis thaliana
Visit the PubMed ArticleAt4g37760 ... was also increased in the gra-D mutants
Horiguchi G, Gonzalez N, Beemster GT, Inzé D, Tsukaya H - Impact of segmental chromosomal duplications on leaf size in the grandifolia-D mutants of Arabidopsis thaliana
Visit the PubMed Articlethe brassinosteroid receptor BRI1 (At4g39400) (Li and Chory, 1997) ... showed elevated expression levels in all three gra-D mutants (P < 0.05, Table S1)
Horiguchi G, Gonzalez N, Beemster GT, Inzé D, Tsukaya H - Impact of segmental chromosomal duplications on leaf size in the grandifolia-D mutants of Arabidopsis thaliana
Visit the PubMed ArticleRT-PCR analysis confirmed that the ANT ... transcripts were more abundant in the gra-D mutants than in the wild-type
Horiguchi G, Gonzalez N, Beemster GT, Inzé D, Tsukaya H - Impact of segmental chromosomal duplications on leaf size in the grandifolia-D mutants of Arabidopsis thaliana
Visit the PubMed Articlein the gra-D mutants ... the expression of ARGOS, which acts upstream of ANT (Hu et al., 2003) and is located on chromosome 3 ... were normal
Horiguchi G, Gonzalez N, Beemster GT, Inzé D, Tsukaya H - Impact of segmental chromosomal duplications on leaf size in the grandifolia-D mutants of Arabidopsis thaliana
Visit the PubMed ArticleRT-PCR analysis confirmed that the ... CYCD3;1 transcripts were more abundant in the gra-D mutants than in the wild-type
Horiguchi G, Gonzalez N, Beemster GT, Inzé D, Tsukaya H - Impact of segmental chromosomal duplications on leaf size in the grandifolia-D mutants of Arabidopsis thaliana
Visit the PubMed Articlewe further analyzed the expression array data. In the commonly duplicated regions, we found four cyclin genes, CYCD3;1 (At4g34160), CYCB1;1 (At4g37490), CYCB2;2 (At4g35620) and CYCD5;1 (At4g37630) (Vandepoele et al., 2002) ... The expression of these genes in at least two gra-D mutants was more than 1.36-fold higher than that in wild-type (P < 0.05, Table S1)
Horiguchi G, Gonzalez N, Beemster GT, Inzé D, Tsukaya H - Impact of segmental chromosomal duplications on leaf size in the grandifolia-D mutants of Arabidopsis thaliana
Visit the PubMed ArticleImmunoblot analyses ... against ... D2 ... dramatically reduced
Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis
Visit the PubMed Articlethe expression of ... ndhG ... reduced in emb1303-2
Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis
Visit the PubMed ArticleGUS) staining on transgenic plants expressing GUS driven by the EMB1303 promoter ... GUS activity was ... low in stems
Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis
Visit the PubMed ArticleSemi-quantitative RT-PCR analysis showed that the levels of ... GGRS transcripts were greatly decreased in emb1303-2
Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis
Visit the PubMed Articlethe expression of ... rbcL ... reduced in emb1303-2
Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis
Visit the PubMed Articlethe expression of ... psaA ... reduced in emb1303-2
Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis
Visit the PubMed Articlethe expression of ... psbG ... reduced in emb1303-2
Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis
Visit the PubMed ArticleSemi-quantitative RT-PCR analysis showed that the levels of PORB ... transcripts were greatly decreased in emb1303-2
Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis
Visit the PubMed Articlethe expression of ... psbD ... reduced in emb1303-2
Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis
Visit the PubMed Articlethe expression of ... psbE ... reduced in emb1303-2
Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis
Visit the PubMed Articlewe analyzed protein profile of emb1303-2. A significant reduction of the large subunit of Rubisco (RBCL) was observed by Coomassie brilliant blue staining
Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis
Visit the PubMed ArticleImmunoblot analyses ... against ... AtpB ... dramatically reduced
Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis
Visit the PubMed ArticleGUS) staining on transgenic plants expressing GUS driven by the EMB1303 promoter ... GUS activity was high in ... young rosette leaves
Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis
Visit the PubMed Articlethe expression of ... psbB ... reduced in emb1303-2
Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis
Visit the PubMed ArticleImmunoblot analyses ... against ... LHCII ... barely detectable in emb1303-2
Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis
Visit the PubMed ArticleSemi-quantitative RT-PCR analysis showed that the levels of ... PORC ... transcripts were greatly decreased in emb1303-2
Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis
Visit the PubMed Articlethe expression of ... psbT ... reduced in emb1303-2
Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis
Visit the PubMed Articlethe expression of ... psbA ... reduced in emb1303-2
Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis
Visit the PubMed ArticleSemi-quantitative RT-PCR analysis ... CLA1 ... expression was also reduced in the mutant
Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis
Visit the PubMed ArticleImmunoblot analyses ... against ... PsbO ... dramatically reduced
Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis
Visit the PubMed ArticleGUS) staining on transgenic plants expressing GUS driven by the EMB1303 promoter ... GUS activity was high in cotyledons
Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis
Visit the PubMed Articlethe expression of ... psaB ... reduced in emb1303-2
Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis
Visit the PubMed Articletranscript levels of accD ... remained the same in the mutant
Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis
Visit the PubMed ArticleImmunoblot analyses ... against ... D1 ... barely detectable in emb1303-2
Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis
Visit the PubMed ArticleSemi-quantitative RT-PCR analysis showed that the levels of ... CAO ... transcripts were greatly decreased in emb1303-2
Huang X, Zhang X, Yang S - A novel chloroplast-localized protein EMB1303 is required for chloroplast development in Arabidopsis
Visit the PubMed ArticleTotal RNA was isolated from the first two leaves of 6-d-old wild-type and gif1/2/3 triple mutant plants, in which their cell proliferation occurred at a maximum rate, and subjected to RT-PCR. The transcript levels of ... CycB1;1 ... were significantly reduced in the triple mutant
Lee BH, Ko JH, Lee S, Lee Y, Pak JH, Kim JH - The Arabidopsis GRF-INTERACTING FACTOR gene family performs an overlapping function in determining organ size as well as multiple developmental properties
Visit the PubMed ArticleTotal RNA was isolated from the first two leaves of 6-d-old wild-type and gif1/2/3 triple mutant plants, in which their cell proliferation occurred at a maximum rate, and subjected to RT-PCR. The transcript levels of ... CycD3;1 ... were significantly reduced in the triple mutant
Lee BH, Ko JH, Lee S, Lee Y, Pak JH, Kim JH - The Arabidopsis GRF-INTERACTING FACTOR gene family performs an overlapping function in determining organ size as well as multiple developmental properties
Visit the PubMed ArticleTotal RNA was isolated from the first two leaves of 6-d-old wild-type and gif1/2/3 triple mutant plants, in which their cell proliferation occurred at a maximum rate, and subjected to RT-PCR. The transcript levels of ... a cyclin-dependent kinase gene, Cdc2b, were significantly reduced in the triple mutant
Lee BH, Ko JH, Lee S, Lee Y, Pak JH, Kim JH - The Arabidopsis GRF-INTERACTING FACTOR gene family performs an overlapping function in determining organ size as well as multiple developmental properties
Visit the PubMed ArticleTotal RNA was isolated from the first two leaves of 6-d-old wild-type and gif1/2/3 triple mutant plants, in which their cell proliferation occurred at a maximum rate, and subjected to RT-PCR ... The S-phase-specific proliferating cell nuclear antigen PCNA1 ... was remarkably down-regulated as well
Lee BH, Ko JH, Lee S, Lee Y, Pak JH, Kim JH - The Arabidopsis GRF-INTERACTING FACTOR gene family performs an overlapping function in determining organ size as well as multiple developmental properties
Visit the PubMed ArticleIn Arabidopsis, organ polarity regulation has been characterized extensively at the molecular level. We therefore have monitored the expression levels of a selection of Arabidopsis genes known to be involved in abaxial/adaxial patterning. The selection of genes comprises ... AS2 ... None of the monitored Arabidopsis genes exhibited significant changes in transcript levels between wox1 prs mutant samples and the wild type
Vandenbussche M, Horstman A, Zethof J, Koes R, Rijpkema AS, Gerats T - Differential recruitment of WOX transcription factors for lateral development and organ fusion in Petunia and Arabidopsis
Visit the PubMed ArticleIn Arabidopsis, organ polarity regulation has been characterized extensively at the molecular level. We therefore have monitored the expression levels of a selection of Arabidopsis genes known to be involved in abaxial/adaxial patterning. The selection of genes comprises ... ASYMMETRIC LEAVES1 (AS1 ... None of the monitored Arabidopsis genes exhibited significant changes in transcript levels between wox1 prs mutant samples and the wild type
Vandenbussche M, Horstman A, Zethof J, Koes R, Rijpkema AS, Gerats T - Differential recruitment of WOX transcription factors for lateral development and organ fusion in Petunia and Arabidopsis
Visit the PubMed ArticleIn Arabidopsis, organ polarity regulation has been characterized extensively at the molecular level. We therefore have monitored the expression levels of a selection of Arabidopsis genes known to be involved in abaxial/adaxial patterning. The selection of genes comprises ... REVOLUTA ... None of the monitored Arabidopsis genes exhibited significant changes in transcript levels between wox1 prs mutant samples and the wild type
Vandenbussche M, Horstman A, Zethof J, Koes R, Rijpkema AS, Gerats T - Differential recruitment of WOX transcription factors for lateral development and organ fusion in Petunia and Arabidopsis
Visit the PubMed ArticleIn Arabidopsis, organ polarity regulation has been characterized extensively at the molecular level. We therefore have monitored the expression levels of a selection of Arabidopsis genes known to be involved in abaxial/adaxial patterning. The selection of genes comprises ... KANADI (KAN ... None of the monitored Arabidopsis genes exhibited significant changes in transcript levels between wox1 prs mutant samples and the wild type
Vandenbussche M, Horstman A, Zethof J, Koes R, Rijpkema AS, Gerats T - Differential recruitment of WOX transcription factors for lateral development and organ fusion in Petunia and Arabidopsis
Visit the PubMed ArticleIn Arabidopsis, organ polarity regulation has been characterized extensively at the molecular level. We therefore have monitored the expression levels of a selection of Arabidopsis genes known to be involved in abaxial/adaxial patterning. The selection of genes comprises ... ETTIN (ETT ... None of the monitored Arabidopsis genes exhibited significant changes in transcript levels between wox1 prs mutant samples and the wild type
Vandenbussche M, Horstman A, Zethof J, Koes R, Rijpkema AS, Gerats T - Differential recruitment of WOX transcription factors for lateral development and organ fusion in Petunia and Arabidopsis
Visit the PubMed ArticleNone of the monitored Arabidopsis genes exhibited significant changes in transcript levels between wox1 prs mutant samples and the wild type, except for the ARF4 gene, which was moderately upregulated in wox1 prs mutants (Figure 6A ). Although only a subtle effect, upregulation of the abaxial cell fate–promoting ARF4 gene is consistent with our morphological observations
Vandenbussche M, Horstman A, Zethof J, Koes R, Rijpkema AS, Gerats T - Differential recruitment of WOX transcription factors for lateral development and organ fusion in Petunia and Arabidopsis
Visit the PubMed ArticleIn Arabidopsis, organ polarity regulation has been characterized extensively at the molecular level. We therefore have monitored the expression levels of a selection of Arabidopsis genes known to be involved in abaxial/adaxial patterning. The selection of genes comprises FILAMENTOUS FLOWER/YABBY1 ... YAB1 ... None of the monitored Arabidopsis genes exhibited significant changes in transcript levels between wox1 prs mutant samples and the wild type
Vandenbussche M, Horstman A, Zethof J, Koes R, Rijpkema AS, Gerats T - Differential recruitment of WOX transcription factors for lateral development and organ fusion in Petunia and Arabidopsis
Visit the PubMed ArticleAt2g39360 ... slightly upregulated by BL treatment in the wild-type
Guo H, Ye H, Li L, Yin Y - A family of receptor-like kinases are regulated by BES1 and involved in plant growth in Arabidopsis thaliana
Visit the PubMed ArticleAt2g39360 ... more clearly increased in bes1-D mutant
Guo H, Ye H, Li L, Yin Y - A family of receptor-like kinases are regulated by BES1 and involved in plant growth in Arabidopsis thaliana
Visit the PubMed ArticleAt5g24010 ... slightly upregulated by BL treatment in the wild-type
Guo H, Ye H, Li L, Yin Y - A family of receptor-like kinases are regulated by BES1 and involved in plant growth in Arabidopsis thaliana
Visit the PubMed ArticleAt1g30570 (HERK2 ... slightly upregulated by BL treatment in the wild-type
Guo H, Ye H, Li L, Yin Y - A family of receptor-like kinases are regulated by BES1 and involved in plant growth in Arabidopsis thaliana
Visit the PubMed ArticleAt1g30570 (HERK2 ... more clearly increased in bes1-D mutant
Guo H, Ye H, Li L, Yin Y - A family of receptor-like kinases are regulated by BES1 and involved in plant growth in Arabidopsis thaliana
Visit the PubMed ArticleAt5g24010 ... more clearly increased in bes1-D mutant
Guo H, Ye H, Li L, Yin Y - A family of receptor-like kinases are regulated by BES1 and involved in plant growth in Arabidopsis thaliana
Visit the PubMed ArticleMYB23 (At5g40330 ... downregulated in the ang3 mutant
Van Minnebruggen A, Neyt P, De Groeve S, Coussens G, Ponce MR, Micol JL, Van Lijsebettens M - The ang3 mutation identified the ribosomal protein gene RPL5B with a role in cell expansion during organ growth
Visit the PubMed ArticleWe analyzed the levels of AtIPT7 transcripts in the above-ground parts of 10-day-old ... plants by real-time qRT-PCR ... the level of the transcript was 1.5-fold higher in 35S:BP plants than in the wild-type plants
Ikezaki M, Kojima M, Sakakibara H, Kojima S, Ueno Y, Machida C, Machida Y - Genetic networks regulated by ASYMMETRIC LEAVES1 (AS1) and AS2 in leaf development in Arabidopsis thaliana: KNOX genes control five morphological events
Visit the PubMed ArticleIn as1 ... levels of ETT/ARF3 transcripts are elevated ... We analyzed the levels of ETT transcripts in the shoot apices of as1-1 ... mutant plants by real-time qRT-PCR
Ikezaki M, Kojima M, Sakakibara H, Kojima S, Ueno Y, Machida C, Machida Y - Genetic networks regulated by ASYMMETRIC LEAVES1 (AS1) and AS2 in leaf development in Arabidopsis thaliana: KNOX genes control five morphological events
Visit the PubMed Articlethe level of AtGA20ox1 transcripts in as1-1 plants was only 50% of that in the wild type
Ikezaki M, Kojima M, Sakakibara H, Kojima S, Ueno Y, Machida C, Machida Y - Genetic networks regulated by ASYMMETRIC LEAVES1 (AS1) and AS2 in leaf development in Arabidopsis thaliana: KNOX genes control five morphological events
Visit the PubMed ArticleIn as1 and as2 mutants, levels of ETT/ARF3 transcripts are elevated (Iwakawa et al., 2007; Figure 6l). We analyzed the levels of ETT transcripts in the shoot apices of ... as1-1 knat126 ... mutant plants by real-time qRT-PCR. As shown in Figure 6(l), the levels of ETT transcripts were similar in all plants examined, suggesting that the elevated levels of ETT transcripts in as1-1 and as2-1 plants were not caused by the ectopically expressed KNAT126 genes
Ikezaki M, Kojima M, Sakakibara H, Kojima S, Ueno Y, Machida C, Machida Y - Genetic networks regulated by ASYMMETRIC LEAVES1 (AS1) and AS2 in leaf development in Arabidopsis thaliana: KNOX genes control five morphological events
Visit the PubMed ArticleWe analyzed the levels of AtIPT7 transcripts in the above-ground parts of 10-day-old as1-1 mutant ... by real-time qRT-PCR. As shown in Figure 5(g), there was no obvious difference, in terms of the level of the AtIPT7 transcript, between wild-type and mutant plants
Ikezaki M, Kojima M, Sakakibara H, Kojima S, Ueno Y, Machida C, Machida Y - Genetic networks regulated by ASYMMETRIC LEAVES1 (AS1) and AS2 in leaf development in Arabidopsis thaliana: KNOX genes control five morphological events
Visit the PubMed ArticleWe analyzed the levels of AtGA20ox1 transcripts in the aerial parts of ... as1-1 plus knat126 mutant plants by real-time quantitative RT-PCR ... When a mutation in each KNAT126 gene was introduced separately into as1-1 plants, the level of the transcript returned to close to control values. These results suggest that the ectopically expressed KNAT126 genes in as1-1 plants might repress the expression of AtGA20ox1 in a redundant manner
Ikezaki M, Kojima M, Sakakibara H, Kojima S, Ueno Y, Machida C, Machida Y - Genetic networks regulated by ASYMMETRIC LEAVES1 (AS1) and AS2 in leaf development in Arabidopsis thaliana: KNOX genes control five morphological events
Visit the PubMed ArticleIn ... as2 mutants, levels of ETT/ARF3 transcripts are elevated ... We analyzed the levels of ETT transcripts in the shoot apices of ... as2-1 ... mutant plants by real-time qRT-PCR
Ikezaki M, Kojima M, Sakakibara H, Kojima S, Ueno Y, Machida C, Machida Y - Genetic networks regulated by ASYMMETRIC LEAVES1 (AS1) and AS2 in leaf development in Arabidopsis thaliana: KNOX genes control five morphological events
Visit the PubMed ArticleWe analyzed the levels of AtIPT7 transcripts in the above-ground parts of 10-day-old ... as1-1 plus knat126 mutant plants by real-time qRT-PCR. As shown in Figure 5(g), there was no obvious difference, in terms of the level of the AtIPT7 transcript, between wild-type and mutant plants
Ikezaki M, Kojima M, Sakakibara H, Kojima S, Ueno Y, Machida C, Machida Y - Genetic networks regulated by ASYMMETRIC LEAVES1 (AS1) and AS2 in leaf development in Arabidopsis thaliana: KNOX genes control five morphological events
Visit the PubMed ArticleIn as1 and as2 mutants, levels of ETT/ARF3 transcripts are elevated (Iwakawa et al., 2007; Figure 6l). We analyzed the levels of ETT transcripts in the shoot apices of ... as2-1 knat126 mutant plants by real-time qRT-PCR. As shown in Figure 6(l), the levels of ETT transcripts were similar in all plants examined, suggesting that the elevated levels of ETT transcripts in as1-1 and as2-1 plants were not caused by the ectopically expressed KNAT126 genes
Ikezaki M, Kojima M, Sakakibara H, Kojima S, Ueno Y, Machida C, Machida Y - Genetic networks regulated by ASYMMETRIC LEAVES1 (AS1) and AS2 in leaf development in Arabidopsis thaliana: KNOX genes control five morphological events
Visit the PubMed ArticleTo determine the expression pattern of SMO2, we generated transgenic plants expressing a pSMO2:GUS fusion gene and examined GUS activities in seedlings and developing organs ... strong GUS staining was observed ... in leaf
Hu Z, Qin Z, Wang M, Xu C, Feng G, Liu J, Meng Z, Hu Y - The Arabidopsis SMO2, a homologue of yeast TRM112, modulates progression of cell division during organ growth
Visit the PubMed ArticleTo determine the expression pattern of SMO2, we generated transgenic plants expressing a pSMO2:GUS fusion gene and examined GUS activities in seedlings and developing organs ... strong GUS staining was observed in shoot ... meristem
Hu Z, Qin Z, Wang M, Xu C, Feng G, Liu J, Meng Z, Hu Y - The Arabidopsis SMO2, a homologue of yeast TRM112, modulates progression of cell division during organ growth
Visit the PubMed Articlewe ... examined the expression of five cell cycle checkpoint-related genes in WT and smo2, including ... HISTONE H4 ... by real-time quantitative reverse-transcriptional polymerase chain reaction (qRT-PCR), and found that the expression ... was elevated in the smo2 mutant
Hu Z, Qin Z, Wang M, Xu C, Feng G, Liu J, Meng Z, Hu Y - The Arabidopsis SMO2, a homologue of yeast TRM112, modulates progression of cell division during organ growth
Visit the PubMed Articlewe ... examined the expression of five cell cycle checkpoint-related genes in WT and smo2, including ... CYCA1;1 ... by real-time quantitative reverse-transcriptional polymerase chain reaction (qRT-PCR), and found that the expression ... was elevated in the smo2 mutant
Hu Z, Qin Z, Wang M, Xu C, Feng G, Liu J, Meng Z, Hu Y - The Arabidopsis SMO2, a homologue of yeast TRM112, modulates progression of cell division during organ growth
Visit the PubMed ArticleNo transcript of At1g22270 was detected in smo2 plants by RT-PCR analysis
Hu Z, Qin Z, Wang M, Xu C, Feng G, Liu J, Meng Z, Hu Y - The Arabidopsis SMO2, a homologue of yeast TRM112, modulates progression of cell division during organ growth
Visit the PubMed Articlewe introduced a pCYCB1;1:Dbox-GUS construct into smo2 ... the most dramatic accumulation of CYCB1;1:GUS protein was observed in smo2 ... juvenile leaves
Hu Z, Qin Z, Wang M, Xu C, Feng G, Liu J, Meng Z, Hu Y - The Arabidopsis SMO2, a homologue of yeast TRM112, modulates progression of cell division during organ growth
Visit the PubMed Articlewe introduced a pCYCB1;1:Dbox-GUS construct into smo2 ... the most dramatic accumulation of CYCB1;1:GUS protein was observed in smo2 leaf primordia
Hu Z, Qin Z, Wang M, Xu C, Feng G, Liu J, Meng Z, Hu Y - The Arabidopsis SMO2, a homologue of yeast TRM112, modulates progression of cell division during organ growth
Visit the PubMed Articlewe ... examined the expression of five cell cycle checkpoint-related genes in WT and smo2, including ... CYCB1;1, by real-time quantitative reverse-transcriptional polymerase chain reaction (qRT-PCR), and found that the expression ... was elevated in the smo2 mutant
Hu Z, Qin Z, Wang M, Xu C, Feng G, Liu J, Meng Z, Hu Y - The Arabidopsis SMO2, a homologue of yeast TRM112, modulates progression of cell division during organ growth
Visit the PubMed ArticleTo determine the expression pattern of SMO2, we generated transgenic plants expressing a pSMO2:GUS fusion gene and examined GUS activities in seedlings and developing organs ... a moderate level of GUS expression was detected in the cotyledon vascular bundles
Hu Z, Qin Z, Wang M, Xu C, Feng G, Liu J, Meng Z, Hu Y - The Arabidopsis SMO2, a homologue of yeast TRM112, modulates progression of cell division during organ growth
Visit the PubMed Articlewe ... examined the expression of five cell cycle checkpoint-related genes in WT and smo2, including ... CYCB2;3 ... by real-time quantitative reverse-transcriptional polymerase chain reaction (qRT-PCR), and found that the expression ... was elevated in the smo2 mutant
Hu Z, Qin Z, Wang M, Xu C, Feng G, Liu J, Meng Z, Hu Y - The Arabidopsis SMO2, a homologue of yeast TRM112, modulates progression of cell division during organ growth
Visit the PubMed Articlewe ... examined the expression of five cell cycle checkpoint-related genes in WT and smo2, including CYCD3;1 ... by real-time quantitative reverse-transcriptional polymerase chain reaction (qRT-PCR), and found that the expression ... was elevated in the smo2 mutant
Hu Z, Qin Z, Wang M, Xu C, Feng G, Liu J, Meng Z, Hu Y - The Arabidopsis SMO2, a homologue of yeast TRM112, modulates progression of cell division during organ growth
Visit the PubMed ArticleFLC transcript levels were reduced in the ssrp1-2
Lolas IB, Himanen K, Grønlund JT, Lynggaard C, Houben A, Melzer M, Van Lijsebettens M, Grasser KD - The transcript elongation factor FACT affects Arabidopsis vegetative and reproductive development and genetically interacts with HUB1/2
Visit the PubMed Articlein the ... spt16-1 ... increased level of SOC1 transcript was detected
Lolas IB, Himanen K, Grønlund JT, Lynggaard C, Houben A, Melzer M, Van Lijsebettens M, Grasser KD - The transcript elongation factor FACT affects Arabidopsis vegetative and reproductive development and genetically interacts with HUB1/2
Visit the PubMed Articlein the ... spt16-2 ... increased level of SOC1 transcript was detected
Lolas IB, Himanen K, Grønlund JT, Lynggaard C, Houben A, Melzer M, Van Lijsebettens M, Grasser KD - The transcript elongation factor FACT affects Arabidopsis vegetative and reproductive development and genetically interacts with HUB1/2
Visit the PubMed ArticleFLC transcript levels were reduced in the ... spt16-1
Lolas IB, Himanen K, Grønlund JT, Lynggaard C, Houben A, Melzer M, Van Lijsebettens M, Grasser KD - The transcript elongation factor FACT affects Arabidopsis vegetative and reproductive development and genetically interacts with HUB1/2
Visit the PubMed Articlethe transcript level of SPT16 is not reduced in ssrp1-2
Lolas IB, Himanen K, Grønlund JT, Lynggaard C, Houben A, Melzer M, Van Lijsebettens M, Grasser KD - The transcript elongation factor FACT affects Arabidopsis vegetative and reproductive development and genetically interacts with HUB1/2
Visit the PubMed ArticleThe SSRP1 and SPT16 protein levels were also analysed by immunoblotting ... the amount of SPT16 was reduced in ... ssrp1-2
Lolas IB, Himanen K, Grønlund JT, Lynggaard C, Houben A, Melzer M, Van Lijsebettens M, Grasser KD - The transcript elongation factor FACT affects Arabidopsis vegetative and reproductive development and genetically interacts with HUB1/2
Visit the PubMed ArticleFLC transcript levels were reduced in the ... spt16-2
Lolas IB, Himanen K, Grønlund JT, Lynggaard C, Houben A, Melzer M, Van Lijsebettens M, Grasser KD - The transcript elongation factor FACT affects Arabidopsis vegetative and reproductive development and genetically interacts with HUB1/2
Visit the PubMed ArticleThe SSRP1 and SPT16 protein levels were also analysed by immunoblotting ... The amount of SSRP1 was slightly reduced in the spt16 lines
Lolas IB, Himanen K, Grønlund JT, Lynggaard C, Houben A, Melzer M, Van Lijsebettens M, Grasser KD - The transcript elongation factor FACT affects Arabidopsis vegetative and reproductive development and genetically interacts with HUB1/2
Visit the PubMed Articlein the ssrp1-2 ... increased level of SOC1 transcript was detected
Lolas IB, Himanen K, Grønlund JT, Lynggaard C, Houben A, Melzer M, Van Lijsebettens M, Grasser KD - The transcript elongation factor FACT affects Arabidopsis vegetative and reproductive development and genetically interacts with HUB1/2
Visit the PubMed ArticleSSRP1 is not reduced in spt16
Lolas IB, Himanen K, Grønlund JT, Lynggaard C, Houben A, Melzer M, Van Lijsebettens M, Grasser KD - The transcript elongation factor FACT affects Arabidopsis vegetative and reproductive development and genetically interacts with HUB1/2
Visit the PubMed Articleweak expression of miR396 at ... leaf primordia
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticleGRFs by RT-qPCR ... were expressed during the early stages of leaf development
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticleGRF2:wtGRF2-GUS ... At later stages of development ... reporter activity ... tapered off towards the distal part of the leaf
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticleDuring the initial stages of leaf development, the wild-type GRF2 reporter displayed a high and homogeneous level of expression
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticleMIR396b ... increased tenfold
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticlemiRNA-regulated GRFs in soj8 ... reduction in the levels of these transcription factors
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articlesoj8 to the GRF2:wtGRF2-GUS plants and observed that the reporter was turned off sooner in the mutant than in wild-type plants
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articledecrease in the level of miR396-regulated GRFs during leaf development
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticleGRFs by RT-qPCR ... were expressed during the early stages of leaf development
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articlelevels of GRF5 ... decreased in soj8
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticlemiRNA-regulated GRFs in ... weak rTCP4 transgenic plants ... reduction in the levels of these transcription factors
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articlein situ hybridization on sections of 15-day-old plants grown in short photoperiods ... expression of GRF2 in ... young developing leaves
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticleCYCLINB1;1 (CYCB1;1 ... expression of these cell cycle markers decreased in a similar way to the GRFs during early leaf development
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticleWe then measured the levels of the GRFs in 35S:miR396b stable transgenics by RT-qPCR ... high levels of miR396 caused a reduction in the expression of the miRNA-regulated transcription factors
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articledecrease in the level of miR396-regulated GRFs during leaf development
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articleweak expression of miR396 at the SAM
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticlemiRNA-regulated GRFs in ... weak rTCP4 transgenic plants ... reduction in the levels of these transcription factors
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articleat intermediate developmental stages, the wild-type GRF2 reporter showed a proximo-distal gradient of expression
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articledecrease in the level of miR396-regulated GRFs during leaf development
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticlemiR396 overexpression ... GRF6 expression was decreased
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticlemiRNA-regulated GRFs in ... weak rTCP4 transgenic plants ... reduction in the levels of these transcription factors
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articlelevels of miR396 and found that they were increased two- to three-fold in the apices of soj8
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articleaerial parts of ... 10-day-old ... KNOLLE ... downregulated in 35S:miR396b
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticleGRF2:wtGRF2-GUS ... we did not observe activity of the wild-type GRF2 reporter in fully expanded leaves
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articledecrease in the level of miR396-regulated GRFs during leaf development
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articlein situ hybridization on sections of 15-day-old plants grown in short photoperiods. We detected miR396 throughout the SAM
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticleGRF5 ... similar expression pattern to the miR396-regulated transcription factors during organ growth
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articlelevels of ... GIF1 ... decreased in soj8
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticleSmall RNA blots confirmed that miR396 accumulated to higher levels in the distal part of the leaf
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articleat intermediate developmental stages ... miRNA-resistant GRF2 reporter ... was expressed throughout the leaf
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articleat seedling stage ... wild-type ... transgenes raised the GRF2 mRNA level several fold
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticleGRFs by RT-qPCR ... were expressed during the early stages of leaf development
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticleGRFs by RT-qPCR ... were expressed during the early stages of leaf development
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articlein situ hybridization on sections of 15-day-old plants grown in short photoperiods. We detected miR396 throughout the ... leaf primordia
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticlemiRNA-regulated GRFs in soj8 ... reduction in the levels of these transcription factors
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticlemiRNA-regulated GRFs in ... weak rTCP4 transgenic plants ... reduction in the levels of these transcription factors
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticlemiR396 overexpression ... GRF5 was largely unaffected
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articlethese results indicated that miR396 accumulates during leaf development
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticlemiRNA-regulated GRFs in soj8 ... reduction in the levels of these transcription factors
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articlefifth rosette leaf ... at three-day intervals, starting from the day that it first became visible (~1 mm) to the naked eye, which was 16 days after sowing (DAS ... small RNA blots ... miR396 was expressed at low levels in young leaves ... and that it steadily accumulated during the development of the organ
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticleGRF2:wtGRF2-GUS was strongly expressed in young leaves
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articlelevels of miR396 and found that they were increased two- to three-fold in the apices of soj8
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticleGRF6 ... similar expression pattern to the miR396-regulated transcription factors during organ growth
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticleCYCB1;1 in plants overexpressing miR396 and observed that high levels of the miRNA greatly reduced the number of CYCB1;1 positive cells in developing leaves
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articlethese results indicated that miR396 accumulates during leaf development
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticleSmall RNA blots confirmed that miR396 accumulated to higher levels in the distal part of the leaf
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articleweak expression of miR396 at the SAM
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articleexpression domain of GRF2:rGRF2-GUS was extended in the leaves of 14 out of 20 T1 lines (Fig. 2C), indicating that the GRF2 promoter is apparently uniformly active throughout young leaves
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articleweak rTCP4 transgenic plants ... reduction in the levels of these transcription factors
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articleweak expression of miR396 at ... leaf primordia
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticleWe then measured the levels of the GRFs in 35S:miR396b stable transgenics by RT-qPCR ... high levels of miR396 caused a reduction in the expression of the miRNA-regulated transcription factors
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticleWe then measured the levels of the GRFs in 35S:miR396b stable transgenics by RT-qPCR ... high levels of miR396 caused a reduction in the expression of the miRNA-regulated transcription factors
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticleKNOLLE ... expression of these cell cycle markers decreased in a similar way to the GRFs during early leaf development
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticlemiRNA-regulated GRFs in ... weak rTCP4 transgenic plants ... reduction in the levels of these transcription factors
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticleGRFs by RT-qPCR ... were expressed during the early stages of leaf development
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticlemiR396 ... was expressed at the highest level in the oldest leaf
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticlemiR396 ... was expressed at the highest level in the oldest leaf
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticlemiRNA-regulated GRFs in soj8 ... reduction in the levels of these transcription factors
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articlelevels of ... GRF6 ... decreased in soj8
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticleMIR396a precursor ... was also upregulated during organogenesis
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticleRT-qPCR ... miR396 levels increased up to 30-fold during leaf development
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articledecrease in the level of miR396-regulated GRFs during leaf development
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articlelevels of ... GRF6 ... decreased in ... rTCP4
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articlewild-type GRF2 reporter ... in mature leaves ... was turned off
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articlelevels of miR396 and found that they were increased two- to three-fold in the apices of ... TCP:rTCP4-GFP (rTCP4
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticleWe then measured the levels of the GRFs in 35S:miR396b stable transgenics by RT-qPCR ... high levels of miR396 caused a reduction in the expression of the miRNA-regulated transcription factors
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticleGRF2:wtGRF2-GUS ... At later stages of development, reporter activity was detected mainly at the base of the leaf, with a gradient that tapered off towards the distal part of the leaf
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articlein situ hybridization on sections of 15-day-old plants grown in short photoperiods. We detected miR396 throughout the SAM
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticleNULL
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articlelevels of miR396 and found that they were increased two- to three-fold in the apices of ... TCP:rTCP4-GFP (rTCP4
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticlemiRNA-regulated GRFs in soj8 ... reduction in the levels of these transcription factors
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articlein situ hybridization on sections of 15-day-old plants grown in short photoperiods ... expression of GRF2 in the meristem
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticleGRFs by RT-qPCR ... were expressed during the early stages of leaf development
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articledecrease in the level of miR396-regulated GRFs during leaf development
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticlemiRNA-regulated GRFs in ... weak rTCP4 transgenic plants ... reduction in the levels of these transcription factors
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articlein situ hybridization on sections of 15-day-old plants grown in short photoperiods. We detected miR396 throughout the ... leaf primordia
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articleat seedling stage ... rGRF2 version led to higher levels of mRNA accumulation compared to the wild-type transgene, up to 20-fold
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticleWe then measured the levels of the GRFs in 35S:miR396b stable transgenics by RT-qPCR ... high levels of miR396 caused a reduction in the expression of the miRNA-regulated transcription factors
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticleMIR396a ... increased tenfold
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticlemiRNA-regulated GRFs in soj8 ... reduction in the levels of these transcription factors
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articlelevels of GRF5 ... decreased in ... rTCP4
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articlefifth rosette leaf at three-day intervals, starting from the day that it first became visible (~1 mm) to the naked eye, which was 16 days after sowing (DAS ... small RNA blots ... miR396 was expressed at low levels in young leaves and that it steadily accumulated during the development of the organ
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticleWe then measured the levels of the GRFs in 35S:miR396b stable transgenics by RT-qPCR ... high levels of miR396 caused a reduction in the expression of the miRNA-regulated transcription factors
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticlemiRNA-regulated GRFs in soj8 ... reduction in the levels of these transcription factors
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articlelevels of ... GIF1 ... decreased in ... rTCP4
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticleWe then measured the levels of the GRFs in 35S:miR396b stable transgenics by RT-qPCR ... high levels of miR396 caused a reduction in the expression of the miRNA-regulated transcription factors
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticlemiRNA-regulated GRFs in ... weak rTCP4 transgenic plants ... reduction in the levels of these transcription factors
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticleGRFs by RT-qPCR ... were expressed during the early stages of leaf development
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed Articledecrease in the level of miR396-regulated GRFs during leaf development
Rodriguez RE, Mecchia MA, Debernardi JM, Schommer C, Weigel D, Palatnik JF - Control of cell proliferation in Arabidopsis thaliana by microRNA miR396
Visit the PubMed ArticleSPT expression was ... weakly detected in mature leaves
Ichihashi Y, Horiguchi G, Gleissberg S, Tsukaya H - The bHLH transcription factor SPATULA controls final leaf size in Arabidopsis thaliana
Visit the PubMed Articlewe also found that an3 mutations slightly, but reproducibly, decreased the expression of AtGRF6
Ichihashi Y, Horiguchi G, Gleissberg S, Tsukaya H - The bHLH transcription factor SPATULA controls final leaf size in Arabidopsis thaliana
Visit the PubMed ArticleSPT expression was strongly detected in shoot tips that include young leaf primordia
Ichihashi Y, Horiguchi G, Gleissberg S, Tsukaya H - The bHLH transcription factor SPATULA controls final leaf size in Arabidopsis thaliana
Visit the PubMed Articlewe also found that an3 mutations slightly, but reproducibly, decreased the expression of ... AtGRF9
Ichihashi Y, Horiguchi G, Gleissberg S, Tsukaya H - The bHLH transcription factor SPATULA controls final leaf size in Arabidopsis thaliana
Visit the PubMed Articleapplying exogenous auxin ... We observed a reduced response of the DR5-GUS reporter in the leaves of ron1-1 DR5-GUS plants compared with the response of a nonmutant control
Robles P, Fleury D, Candela H, Cnops G, Alonso-Peral MM, Anami S, Falcone A, Caldana C, Willmitzer L, Ponce MR, Van Lijsebettens M, Micol JL - The RON1/FRY1/SAL1 gene is required for leaf morphogenesis and venation patterning in Arabidopsis
Visit the PubMed ArticleDR5-GUS expression ... in ron1-1 leaf primordia ... was consistently reduced compared with the wild type
Robles P, Fleury D, Candela H, Cnops G, Alonso-Peral MM, Anami S, Falcone A, Caldana C, Willmitzer L, Ponce MR, Van Lijsebettens M, Micol JL - The RON1/FRY1/SAL1 gene is required for leaf morphogenesis and venation patterning in Arabidopsis
Visit the PubMed ArticleRON1/FRY1 is expressed in ... the leaves
Robles P, Fleury D, Candela H, Cnops G, Alonso-Peral MM, Anami S, Falcone A, Caldana C, Willmitzer L, Ponce MR, Van Lijsebettens M, Micol JL - The RON1/FRY1/SAL1 gene is required for leaf morphogenesis and venation patterning in Arabidopsis
Visit the PubMed ArticleRON1/FRY1 ... highest expression signal detected in the leaves occurs in the veins
Robles P, Fleury D, Candela H, Cnops G, Alonso-Peral MM, Anami S, Falcone A, Caldana C, Willmitzer L, Ponce MR, Van Lijsebettens M, Micol JL - The RON1/FRY1/SAL1 gene is required for leaf morphogenesis and venation patterning in Arabidopsis
Visit the PubMed ArticleDR5-GUS expression was weak ... at the basal portion of ron1-1 leaves
Robles P, Fleury D, Candela H, Cnops G, Alonso-Peral MM, Anami S, Falcone A, Caldana C, Willmitzer L, Ponce MR, Van Lijsebettens M, Micol JL - The RON1/FRY1/SAL1 gene is required for leaf morphogenesis and venation patterning in Arabidopsis
Visit the PubMed ArticleIn ron1-1 leaves, ATHB-8-GUS revealed an open network with supernumerary free-ending veins
Robles P, Fleury D, Candela H, Cnops G, Alonso-Peral MM, Anami S, Falcone A, Caldana C, Willmitzer L, Ponce MR, Van Lijsebettens M, Micol JL - The RON1/FRY1/SAL1 gene is required for leaf morphogenesis and venation patterning in Arabidopsis
Visit the PubMed ArticleDR5-GUS expression ... in ron1-1 leaf primordia ... was ... confined to the apical region
Robles P, Fleury D, Candela H, Cnops G, Alonso-Peral MM, Anami S, Falcone A, Caldana C, Willmitzer L, Ponce MR, Van Lijsebettens M, Micol JL - The RON1/FRY1/SAL1 gene is required for leaf morphogenesis and venation patterning in Arabidopsis
Visit the PubMed ArticleRON1 is overexpressed (1.3- to 2.7-fold change) in the continuous vascular ring1 mutant
Robles P, Fleury D, Candela H, Cnops G, Alonso-Peral MM, Anami S, Falcone A, Caldana C, Willmitzer L, Ponce MR, Van Lijsebettens M, Micol JL - The RON1/FRY1/SAL1 gene is required for leaf morphogenesis and venation patterning in Arabidopsis
Visit the PubMed ArticleDR5-GUS expression ... in ron1-1 ... leaf ... persisted in the apex and appeared as a network only in the proximal portion of the leaf
Robles P, Fleury D, Candela H, Cnops G, Alonso-Peral MM, Anami S, Falcone A, Caldana C, Willmitzer L, Ponce MR, Van Lijsebettens M, Micol JL - The RON1/FRY1/SAL1 gene is required for leaf morphogenesis and venation patterning in Arabidopsis
Visit the PubMed ArticleRON1/FRY1 is expressed in ... stems
Robles P, Fleury D, Candela H, Cnops G, Alonso-Peral MM, Anami S, Falcone A, Caldana C, Willmitzer L, Ponce MR, Van Lijsebettens M, Micol JL - The RON1/FRY1/SAL1 gene is required for leaf morphogenesis and venation patterning in Arabidopsis
Visit the PubMed Articleexpression of ... BRCA ... was hyper-induced in etg1-1 ctf18-1 double mutant plants
Takahashi N, Quimbaya M, Schubert V, Lammens T, Vandepoele K, Schubert I, Matsui M, Inzé D, Berx G, De Veylder L - The MCM-binding protein ETG1 aids sister chromatid cohesion required for postreplicative homologous recombination repair
Visit the PubMed ArticleThe spatial expression pattern of CTF18 was analyzed in more than six independent transgenic lines expressing the ß-glucuronidase (GUS) reporter gene under control of the CTF18 promoter. In 7-day-old seedlings, the levels of CTF18 expression were high at the shoot apical and root meristems (Figure 3C and 3D), corresponding with an anticipated role for CTF18 during cell cycle progression
Takahashi N, Quimbaya M, Schubert V, Lammens T, Vandepoele K, Schubert I, Matsui M, Inzé D, Berx G, De Veylder L - The MCM-binding protein ETG1 aids sister chromatid cohesion required for postreplicative homologous recombination repair
Visit the PubMed ArticleTranscriptional activation of the Arabidopsis CTF18 gene in E2Fa-DPa-overexpressing plants was confirmed by quantitative real-time PCR analysis
Takahashi N, Quimbaya M, Schubert V, Lammens T, Vandepoele K, Schubert I, Matsui M, Inzé D, Berx G, De Veylder L - The MCM-binding protein ETG1 aids sister chromatid cohesion required for postreplicative homologous recombination repair
Visit the PubMed Articleexpression of ... CYCB1;1 ... was hyper-induced in etg1-1 ctf18-1 double mutant plants
Takahashi N, Quimbaya M, Schubert V, Lammens T, Vandepoele K, Schubert I, Matsui M, Inzé D, Berx G, De Veylder L - The MCM-binding protein ETG1 aids sister chromatid cohesion required for postreplicative homologous recombination repair
Visit the PubMed Articleexpression of the PARP2 ... was hyper-induced in etg1-1 ctf18-1 double mutant plants
Takahashi N, Quimbaya M, Schubert V, Lammens T, Vandepoele K, Schubert I, Matsui M, Inzé D, Berx G, De Veylder L - The MCM-binding protein ETG1 aids sister chromatid cohesion required for postreplicative homologous recombination repair
Visit the PubMed ArticleUnlike AS1, AS2 was not ectopically expressed in stm-11 embryos until the torpedo stage. In bent-cotyledon stage wild-type embryos, AS2 expression was restricted to the adaxial region of the cotyledons (Figures 7E and 7F). In stm-11 embryos, AS2 was ectopically expressed at the junction between the fused cotyledons, where the SAM would normally have formed (Figures 7G and 7H; see Supplemental Table 2 online). Because ectopic activation of AS2 was observed only after the stm phenotype was manifested, this result indicates that AS2 expression is not directly regulated by STM, but rather by another factor functional in the SAM
Jun JH, Ha CM, Fletcher JC - BLADE-ON-PETIOLE1 coordinates organ determinacy and axial polarity in arabidopsis by directly activating ASYMMETRIC LEAVES2
Visit the PubMed Articlein ... bop1-4 bop2-11 embryos, AS2pro:GUS activity was not detected in the proximal domain of the cotyledons near the SAM (Figures 5B, 5C, 5E, and 5F). Sagittal views confirm that GUS staining was absent from the proximal domain of bop embryonic cotyledons
Jun JH, Ha CM, Fletcher JC - BLADE-ON-PETIOLE1 coordinates organ determinacy and axial polarity in arabidopsis by directly activating ASYMMETRIC LEAVES2
Visit the PubMed ArticleA dual subcellular localization pattern has also been observed for BOP2 (Hepworth et al., 2005), and we likewise detected BOP1-green fluorescent protein fusions in both the nucleus and the cytoplasm
Jun JH, Ha CM, Fletcher JC - BLADE-ON-PETIOLE1 coordinates organ determinacy and axial polarity in arabidopsis by directly activating ASYMMETRIC LEAVES2
Visit the PubMed ArticleNext, we analyzed AS2pro:GUS activity at the vegetative stage. Compared with wild-type seedlings (Figure 5M; see Supplemental Figures 6A and 6B online), GUS activity was reduced or absent in the leaf base of ... bop1-1 ... seedlings ... This region corresponds to the BOP1 and BOP2 expression domains
Jun JH, Ha CM, Fletcher JC - BLADE-ON-PETIOLE1 coordinates organ determinacy and axial polarity in arabidopsis by directly activating ASYMMETRIC LEAVES2
Visit the PubMed ArticleNPR1 has a dual nuclear and cytoplasmic subcellular localization pattern
Jun JH, Ha CM, Fletcher JC - BLADE-ON-PETIOLE1 coordinates organ determinacy and axial polarity in arabidopsis by directly activating ASYMMETRIC LEAVES2
Visit the PubMed ArticleNext, we analyzed AS2pro:GUS activity at the vegetative stage. Compared with wild-type seedlings (Figure 5M; see Supplemental Figures 6A and 6B online), GUS activity was reduced or absent in the leaf base of ... bop1-4 bop2-11 seedlings ... This region corresponds to the BOP1 and BOP2 expression domains
Jun JH, Ha CM, Fletcher JC - BLADE-ON-PETIOLE1 coordinates organ determinacy and axial polarity in arabidopsis by directly activating ASYMMETRIC LEAVES2
Visit the PubMed ArticleWhen fused to a GUS reporter, this BOP1 promoter sequence drives strong GUS activity at the base of the cotyledons and leaves, recapitulating the native BOP1 expression domain
Jun JH, Ha CM, Fletcher JC - BLADE-ON-PETIOLE1 coordinates organ determinacy and axial polarity in arabidopsis by directly activating ASYMMETRIC LEAVES2
Visit the PubMed ArticleBP expression was expanded into the presumptive junction between the hypocotyl and cotyledon base (Figure 7T; see Supplemental Figures 9B and 9C online), the area corresponding to the region of de novo shoot meristem generation in stm-11 bop1-4 bop2-11 seedlings
Jun JH, Ha CM, Fletcher JC - BLADE-ON-PETIOLE1 coordinates organ determinacy and axial polarity in arabidopsis by directly activating ASYMMETRIC LEAVES2
Visit the PubMed ArticleIn as1-1 ... embryos, ectopic BP expression was observed in the basal region of the cotyledons and flanking the SAM
Jun JH, Ha CM, Fletcher JC - BLADE-ON-PETIOLE1 coordinates organ determinacy and axial polarity in arabidopsis by directly activating ASYMMETRIC LEAVES2
Visit the PubMed ArticleWe found that AS2 ... mRNA levels were elevated within 4 and 24 h ... of Dex induction in 35Spro:BOP1-GR bop1-1 ... AS2 expression was induced as early as 1 h after Dex application and continuously increased over the 24-h time course
Jun JH, Ha CM, Fletcher JC - BLADE-ON-PETIOLE1 coordinates organ determinacy and axial polarity in arabidopsis by directly activating ASYMMETRIC LEAVES2
Visit the PubMed Articlein bop1-1 ... embryos, AS2pro:GUS activity was not detected in the proximal domain of the cotyledons near the SAM (Figures 5B, 5C, 5E, and 5F). Sagittal views confirm that GUS staining was absent from the proximal domain of bop embryonic cotyledons
Jun JH, Ha CM, Fletcher JC - BLADE-ON-PETIOLE1 coordinates organ determinacy and axial polarity in arabidopsis by directly activating ASYMMETRIC LEAVES2
Visit the PubMed ArticleWe found that ... ASL4/LOB mRNA levels were elevated within 4 and 24 h ... of Dex induction in 35Spro:BOP1-GR bop1-1 ... over the 24-h time course ... increased ASL4/LOB expression only became detectable 4 to 8 h after induction
Jun JH, Ha CM, Fletcher JC - BLADE-ON-PETIOLE1 coordinates organ determinacy and axial polarity in arabidopsis by directly activating ASYMMETRIC LEAVES2
Visit the PubMed ArticleA dual subcellular localization pattern has also been observed for BOP2 (Hepworth et al., 2005), and we likewise detected BOP1-green fluorescent protein fusions in both the nucleus and the cytoplasm
Jun JH, Ha CM, Fletcher JC - BLADE-ON-PETIOLE1 coordinates organ determinacy and axial polarity in arabidopsis by directly activating ASYMMETRIC LEAVES2
Visit the PubMed ArticleIn ... as2-1 embryos, ectopic BP expression was observed in the basal region of the cotyledons and flanking the SAM
Jun JH, Ha CM, Fletcher JC - BLADE-ON-PETIOLE1 coordinates organ determinacy and axial polarity in arabidopsis by directly activating ASYMMETRIC LEAVES2
Visit the PubMed ArticleAfter 2 h of BOP1 induction, AS2pro:GUS activity in 35Spro:BOP1-GR bop1-1 plants was elevated in developing leaves and expanded more distally into the blade of mature leaves (Figure 3D). After 24 h of induction, ectopic GUS activity was observed throughout the blades of mature leaves and the base of the cotyledons
Jun JH, Ha CM, Fletcher JC - BLADE-ON-PETIOLE1 coordinates organ determinacy and axial polarity in arabidopsis by directly activating ASYMMETRIC LEAVES2
Visit the PubMed ArticleNPR1 has a dual nuclear and cytoplasmic subcellular localization pattern
Jun JH, Ha CM, Fletcher JC - BLADE-ON-PETIOLE1 coordinates organ determinacy and axial polarity in arabidopsis by directly activating ASYMMETRIC LEAVES2
Visit the PubMed Articleectopic BP expression was not observed flanking the shoot apex in bop1-4 bop2-11 embryos
Jun JH, Ha CM, Fletcher JC - BLADE-ON-PETIOLE1 coordinates organ determinacy and axial polarity in arabidopsis by directly activating ASYMMETRIC LEAVES2
Visit the PubMed ArticleTo map the expression of SPATULA throughout the developing plant, a sequence of 6253 bp upstream of the start of transcription to 313 bp downstream was translationally fused to the GUS reporter gene and transformed into wild-type plants ... In germinating seeds (Fig. 1T–Y), expression was first detected 2 d after imbibition of aged seeds (Fig. 1V), and was strong throughout the hypocotyl and cotyledons, and later in the cotyledon's vasculature
Groszmann M, Bylstra Y, Lampugnani ER, Smyth DR - Regulation of tissue-specific expression of SPATULA, a bHLH gene involved in carpel development, seedling germination, and lateral organ growth in Arabidopsis
Visit the PubMed Articleexpression of SPATULA ... As leaf primordia developed, expression was associated with the developing vasculature, and in basal margins (Fig. 1R; see Supplementary Fig. S1 at JXB online). In older developing leaves, continuous expression was seen in basal regions (Fig. 1R, S), and it also occurred in the phloem, hydathodes, and stomata (see Supplementary Fig. 1S at JXB online), falling away as the leaves matured
Groszmann M, Bylstra Y, Lampugnani ER, Smyth DR - Regulation of tissue-specific expression of SPATULA, a bHLH gene involved in carpel development, seedling germination, and lateral organ growth in Arabidopsis
Visit the PubMed Articleexpression of SPATULA ... As seedlings developed, strong expression was observed in the shoot meristem (Fig. 1R), and later in the inflorescence meristem as well
Groszmann M, Bylstra Y, Lampugnani ER, Smyth DR - Regulation of tissue-specific expression of SPATULA, a bHLH gene involved in carpel development, seedling germination, and lateral organ growth in Arabidopsis
Visit the PubMed ArticleSPATULA ... Expression in newly arising leaf primordia did not extend throughout (Fig. 1Y), but was localized to two medial regions, adjacent to and distant from the meristem
Groszmann M, Bylstra Y, Lampugnani ER, Smyth DR - Regulation of tissue-specific expression of SPATULA, a bHLH gene involved in carpel development, seedling germination, and lateral organ growth in Arabidopsis
Visit the PubMed ArticleTo map the expression of SPATULA throughout the developing plant, a sequence of 6253 bp upstream of the start of transcription to 313 bp downstream was translationally fused to the GUS reporter gene and transformed into wild-type plants ... It was observed in the developing embryo from the transition stage through the heart stage, confined to the basal half (Fig. 1N, O). By the torpedo stage it had become concentrated in the developing root meristem and the procambium of the future hypocotyl, and in the newly arisen shoot apical meristem
Groszmann M, Bylstra Y, Lampugnani ER, Smyth DR - Regulation of tissue-specific expression of SPATULA, a bHLH gene involved in carpel development, seedling germination, and lateral organ growth in Arabidopsis
Visit the PubMed ArticleNINJA expression is induced by methyl jasmonate (MeJA) within 1 h and for at least 12 h after elicitation
Pauwels L, Barbero GF, Geerinck J, Tilleman S, Grunewald W, Pérez AC, Chico JM, Bossche RV, Sewell J, Gil E, García-Casado G, Witters E, Inzé D, Long JA, De Jaeger G, Solano R, Goossens A - NINJA connects the co-repressor TOPLESS to jasmonate signalling
Visit the PubMed ArticleAnalysis of seedlings producing a C-terminal GFP fusion with NINJA revealed a clear nuclear localization for NINJA
Pauwels L, Barbero GF, Geerinck J, Tilleman S, Grunewald W, Pérez AC, Chico JM, Bossche RV, Sewell J, Gil E, García-Casado G, Witters E, Inzé D, Long JA, De Jaeger G, Solano R, Goossens A - NINJA connects the co-repressor TOPLESS to jasmonate signalling
Visit the PubMed ArticleIn the rps6b mutant a signal is detected
Creff A, Sormani R, Desnos T - The two Arabidopsis RPS6 genes, encoding for cytoplasmic ribosomal proteins S6, are functionally equivalent
Visit the PubMed Articlethe RPS6 signal in rps6b seedlings is close to the WT level
Creff A, Sormani R, Desnos T - The two Arabidopsis RPS6 genes, encoding for cytoplasmic ribosomal proteins S6, are functionally equivalent
Visit the PubMed ArticleIn the rps6a mutant the RPS6 signal intensity is only ~15% of the WT level
Creff A, Sormani R, Desnos T - The two Arabidopsis RPS6 genes, encoding for cytoplasmic ribosomal proteins S6, are functionally equivalent
Visit the PubMed Articlein the rps6a mutant no RPS6A mRNA is detected
Creff A, Sormani R, Desnos T - The two Arabidopsis RPS6 genes, encoding for cytoplasmic ribosomal proteins S6, are functionally equivalent
Visit the PubMed ArticleWe first confirmed that the steady-state expression levels of BP ... were higher in bop1 bop2 leaves than in wild-type leaves
Ha CM, Jun JH, Fletcher JC - Control of Arabidopsis leaf morphogenesis through regulation of the YABBY and KNOX families of transcription factors
Visit the PubMed ArticleKNAT6 ... expression levels were also higher in fil yab3 leaves than in wild type and bop1 bop2 leaves
Ha CM, Jun JH, Fletcher JC - Control of Arabidopsis leaf morphogenesis through regulation of the YABBY and KNOX families of transcription factors
Visit the PubMed ArticleKNAT2 ... expression levels in bop1 bop2 fil ... leaves were similar to those in bop1 bop2 leaves (Figure 4A), demonstrating that suppression of the bop1 bop2 ectopic outgrowth phenotype by fil ... is not caused by a reduction in ectopic KNOX1 expression
Ha CM, Jun JH, Fletcher JC - Control of Arabidopsis leaf morphogenesis through regulation of the YABBY and KNOX families of transcription factors
Visit the PubMed ArticleKNAT2 ... expression ... was much higher in bop1 bop2 fil yab3 leaves than either bop1 bop2 or fil yab3 leaves (Figure 4A). This result indicates that the BOP and YAB genes act separately to repress KNOX1 transcription in leaf primordia
Ha CM, Jun JH, Fletcher JC - Control of Arabidopsis leaf morphogenesis through regulation of the YABBY and KNOX families of transcription factors
Visit the PubMed ArticleIn bop1 bop2 fil yab3 quadruple mutant leaves, BP::GUS activity was detected in ... the petiole and blade vasculature
Ha CM, Jun JH, Fletcher JC - Control of Arabidopsis leaf morphogenesis through regulation of the YABBY and KNOX families of transcription factors
Visit the PubMed ArticleCompared to wild-type leaves, bop1 bop2 leaves exhibited stronger and more distally expanded BP::GUS activity in the petiole
Ha CM, Jun JH, Fletcher JC - Control of Arabidopsis leaf morphogenesis through regulation of the YABBY and KNOX families of transcription factors
Visit the PubMed ArticleBP ... expression levels in ... bop1 bop2 yab3 leaves were similar to those in bop1 bop2 leaves ... demonstrating that suppression of the bop1 bop2 ectopic outgrowth phenotype by ... yab3 is not caused by a reduction in ectopic KNOX1 expression
Ha CM, Jun JH, Fletcher JC - Control of Arabidopsis leaf morphogenesis through regulation of the YABBY and KNOX families of transcription factors
Visit the PubMed ArticleBP ... expression levels were also higher in fil yab3 leaves than in wild type and bop1 bop2 leaves
Ha CM, Jun JH, Fletcher JC - Control of Arabidopsis leaf morphogenesis through regulation of the YABBY and KNOX families of transcription factors
Visit the PubMed ArticleWe first confirmed that the steady-state expression levels of ... KNAT6 were higher in bop1 bop2 leaves than in wild-type leaves
Ha CM, Jun JH, Fletcher JC - Control of Arabidopsis leaf morphogenesis through regulation of the YABBY and KNOX families of transcription factors
Visit the PubMed ArticleKNAT6 ... expression ... was much higher in bop1 bop2 fil yab3 leaves than either bop1 bop2 or fil yab3 leaves (Figure 4A). This result indicates that the BOP and YAB genes act separately to repress KNOX1 transcription in leaf primordia
Ha CM, Jun JH, Fletcher JC - Control of Arabidopsis leaf morphogenesis through regulation of the YABBY and KNOX families of transcription factors
Visit the PubMed ArticleIn bop1 bop2 fil yab3 quadruple mutant leaves, BP::GUS activity was detected in ... the hydathodes
Ha CM, Jun JH, Fletcher JC - Control of Arabidopsis leaf morphogenesis through regulation of the YABBY and KNOX families of transcription factors
Visit the PubMed ArticleKNAT6 ... expression levels in ... bop1 bop2 yab3 leaves were similar to those in bop1 bop2 leaves (Figure 4A), demonstrating that suppression of the bop1 bop2 ectopic outgrowth phenotype by ... yab3 is not caused by a reduction in ectopic KNOX1 expression
Ha CM, Jun JH, Fletcher JC - Control of Arabidopsis leaf morphogenesis through regulation of the YABBY and KNOX families of transcription factors
Visit the PubMed ArticleKNAT2 ... expression levels were also higher in fil yab3 leaves than in wild type and bop1 bop2 leaves
Ha CM, Jun JH, Fletcher JC - Control of Arabidopsis leaf morphogenesis through regulation of the YABBY and KNOX families of transcription factors
Visit the PubMed ArticleBP ... expression ... was much higher in bop1 bop2 fil yab3 leaves than either bop1 bop2 or fil yab3 leaves (Figure 4A). This result indicates that the BOP and YAB genes act separately to repress KNOX1 transcription in leaf primordia
Ha CM, Jun JH, Fletcher JC - Control of Arabidopsis leaf morphogenesis through regulation of the YABBY and KNOX families of transcription factors
Visit the PubMed ArticleIn fil yab3 leaves, BP::GUS activity was detected strongly in the petiole and more weakly in the vasculature and hydathodes (Figure 4, D and E, arrowheads), and in the distal region of bifurcated leaves
Ha CM, Jun JH, Fletcher JC - Control of Arabidopsis leaf morphogenesis through regulation of the YABBY and KNOX families of transcription factors
Visit the PubMed Articlebop1 bop2 yab3 leaves had strong BP::GUS activity restricted to the proximal region of the petiole (Figure 4, F and G), similar to bop1 bop2 leaves
Ha CM, Jun JH, Fletcher JC - Control of Arabidopsis leaf morphogenesis through regulation of the YABBY and KNOX families of transcription factors
Visit the PubMed ArticleIn fil yab3 leaves, BP::GUS activity was detected strongly in the petiole and more weakly in the vasculature and hydathodes (Figure 4, D and E, arrowheads), and in the distal region of bifurcated leaves
Ha CM, Jun JH, Fletcher JC - Control of Arabidopsis leaf morphogenesis through regulation of the YABBY and KNOX families of transcription factors
Visit the PubMed Articlebop1 bop2 fil ... leaves had strong BP::GUS activity restricted to the proximal region of the petiole (Figure 4, F and G), similar to bop1 bop2 leaves
Ha CM, Jun JH, Fletcher JC - Control of Arabidopsis leaf morphogenesis through regulation of the YABBY and KNOX families of transcription factors
Visit the PubMed ArticleKNAT6 ... expression levels in bop1 bop2 fil ... leaves were similar to those in bop1 bop2 leaves (Figure 4A), demonstrating that suppression of the bop1 bop2 ectopic outgrowth phenotype by fil ... is not caused by a reduction in ectopic KNOX1 expression
Ha CM, Jun JH, Fletcher JC - Control of Arabidopsis leaf morphogenesis through regulation of the YABBY and KNOX families of transcription factors
Visit the PubMed ArticleWe first confirmed that the steady-state expression levels of ... KNAT2 ... were higher in bop1 bop2 leaves than in wild-type leaves
Ha CM, Jun JH, Fletcher JC - Control of Arabidopsis leaf morphogenesis through regulation of the YABBY and KNOX families of transcription factors
Visit the PubMed ArticleIn bop1 bop2 fil yab3 quadruple mutant leaves, BP::GUS activity was detected in a broader domain than in double- and triple-mutant leaves. In addition to the proximal petiole, the petiole and blade vasculature, the hydathodes and the distal region of bifurcated leaves (Figure 4, H and I), bop1 bop2 fil yab3 leaves also showed strong BP::GUS activity in the ectopic organs along the petiole (Figure 4J, arrows). Thus KNOX1 expression is both strongly elevated and spatially expanded in bop1 bop2 fil yab3 leaves, and thus may play an important role in conditioning the extensive ectopic organ development seen in bop1 bop2 fil yab3 leaves
Ha CM, Jun JH, Fletcher JC - Control of Arabidopsis leaf morphogenesis through regulation of the YABBY and KNOX families of transcription factors
Visit the PubMed ArticleBP ... expression levels in bop1 bop2 fil ... leaves were similar to those in bop1 bop2 leaves (Figure 4A), demonstrating that suppression of the bop1 bop2 ectopic outgrowth phenotype by fil ... is not caused by a reduction in ectopic KNOX1 expression
Ha CM, Jun JH, Fletcher JC - Control of Arabidopsis leaf morphogenesis through regulation of the YABBY and KNOX families of transcription factors
Visit the PubMed ArticleKNAT2 ... expression levels in ... bop1 bop2 yab3 leaves were similar to those in bop1 bop2 leaves (Figure 4A), demonstrating that suppression of the bop1 bop2 ectopic outgrowth phenotype by ... yab3 is not caused by a reduction in ectopic KNOX1 expression
Ha CM, Jun JH, Fletcher JC - Control of Arabidopsis leaf morphogenesis through regulation of the YABBY and KNOX families of transcription factors
Visit the PubMed ArticleAt4g35760 ... Transcripts were also detected in cotyledons, siliques and flowers, although at much lower levels than in leaves, and were undetectable in roots
Furt F, Oostende Cv, Widhalm JR, Dale MA, Wertz J, Basset GJ - A bimodular oxidoreductase mediates the specific reduction of phylloquinone (vitamin K1) in chloroplasts
Visit the PubMed ArticleAt4g35760 protein ... was most abundant in cauline and rosette leaves, with lower levels in cotyledons and stems, and was barely detectable in green siliques
Furt F, Oostende Cv, Widhalm JR, Dale MA, Wertz J, Basset GJ - A bimodular oxidoreductase mediates the specific reduction of phylloquinone (vitamin K1) in chloroplasts
Visit the PubMed ArticleAnalyses of mRNA levels using semiquantitative RT-PCR showed that the transcripts of At4g35760 were most abundant in cauline and mature leaves, and stems
Furt F, Oostende Cv, Widhalm JR, Dale MA, Wertz J, Basset GJ - A bimodular oxidoreductase mediates the specific reduction of phylloquinone (vitamin K1) in chloroplasts
Visit the PubMed ArticleTo obtain experimental evidence for such a plastid localization, the full-length cDNA of the Arabidopsis homolog was fused to the N terminus of the GFP reporter protein and transiently expressed in tobacco mesophyll cells (Figure 2). The green fluorescence of the GFP construct resulted in a distinctive punctate pattern that co-localized with the red autofluorescence of chlorophyll in plastids (Figure 2a–c), whereas the GFP-only control displayed fluorescence confined to the cytoplasm and the nucleus (Figure 2d–f ... Subcellular localization of At4g35760
Furt F, Oostende Cv, Widhalm JR, Dale MA, Wertz J, Basset GJ - A bimodular oxidoreductase mediates the specific reduction of phylloquinone (vitamin K1) in chloroplasts
Visit the PubMed ArticleAttempts to express Arabidopsis VKORC1-Trx-like in Escherichia coli were unsuccessful (data not shown). On the other hand, expression of a 6Xhis-tagged version of its Trx-like domain alone (L231 [RIGHTWARDS ARROW] M) in E. coli BL21-codonPlus cells resulted in the production of large quantities of soluble recombinant protein (data not shown). A polyclonal antiserum was raised against this recombinant moiety and used to probe immunoblots corresponding to the same organs than those analyzed for transcript levels (Figure 3b). A 35-kDa immunosignal corresponding to the calculated mass of the At4g35760 protein minus its chloroplast targeting pre-sequence was detected in all organs tested but flowers and roots. In agreement with the transcript levels, the protein was most abundant in cauline and rosette leaves
Furt F, Oostende Cv, Widhalm JR, Dale MA, Wertz J, Basset GJ - A bimodular oxidoreductase mediates the specific reduction of phylloquinone (vitamin K1) in chloroplasts
Visit the PubMed ArticleAs shown in Figure 5, the transcription activities of accD, clpP and rpoB appear to be slightly altered in the mutant (rpoB increased, and accD and clpP decreased by less than 10%, respectively), whereas those of psbA, psbB and rrn16 were reduced more than 10-fold in the mutants compared with the wild type. These results indicated that the changes in transcription activity may account for the reduced accumulation of PEP-dependent chloroplast gene transcripts in dg1
Chi W, Mao J, Li Q, Ji D, Zou M, Lu C, Zhang L - Interaction of the pentatricopeptide-repeat protein DELAYED GREENING 1 with sigma factor SIG6 in the regulation of chloroplast gene expression in Arabidopsis cotyledons
Visit the PubMed Articlethe psbC levels of sig6 and dg1 were reduced to about 20% of the wild-type levels, whereas that of the double mutant was reduced to about 10% of the wild-type level
Chi W, Mao J, Li Q, Ji D, Zou M, Lu C, Zhang L - Interaction of the pentatricopeptide-repeat protein DELAYED GREENING 1 with sigma factor SIG6 in the regulation of chloroplast gene expression in Arabidopsis cotyledons
Visit the PubMed ArticleIn the sig6 dg1 protoplasts, only the expression of SIG6 can induce the accumulation of PEP-dependent gene transcripts
Chi W, Mao J, Li Q, Ji D, Zou M, Lu C, Zhang L - Interaction of the pentatricopeptide-repeat protein DELAYED GREENING 1 with sigma factor SIG6 in the regulation of chloroplast gene expression in Arabidopsis cotyledons
Visit the PubMed ArticlepsbC levels of sig6 ... were reduced to about 20% of the wild-type levels
Chi W, Mao J, Li Q, Ji D, Zou M, Lu C, Zhang L - Interaction of the pentatricopeptide-repeat protein DELAYED GREENING 1 with sigma factor SIG6 in the regulation of chloroplast gene expression in Arabidopsis cotyledons
Visit the PubMed Articlethe transcript levels of the class-II genes ... rrn23 ... were found to be decreased ... in the ... sig6 ... mutant plants
Chi W, Mao J, Li Q, Ji D, Zou M, Lu C, Zhang L - Interaction of the pentatricopeptide-repeat protein DELAYED GREENING 1 with sigma factor SIG6 in the regulation of chloroplast gene expression in Arabidopsis cotyledons
Visit the PubMed ArticleIn the sig6 protoplasts, expression of SIG6 led to a drastic increase in the levels of PEP-dependent chloroplast gene transcripts, whereas expression of DG1 did not increase the levels of these transcripts
Chi W, Mao J, Li Q, Ji D, Zou M, Lu C, Zhang L - Interaction of the pentatricopeptide-repeat protein DELAYED GREENING 1 with sigma factor SIG6 in the regulation of chloroplast gene expression in Arabidopsis cotyledons
Visit the PubMed ArticleWhen SIG1 was fused to the intact YFP moieties, YFP fluorescence was observed in the chloroplast of the protoplasts (Figure 1b), which suggested that SIG1-fused protein was expressed and properly localized in the chloroplast
Chi W, Mao J, Li Q, Ji D, Zou M, Lu C, Zhang L - Interaction of the pentatricopeptide-repeat protein DELAYED GREENING 1 with sigma factor SIG6 in the regulation of chloroplast gene expression in Arabidopsis cotyledons
Visit the PubMed Articlethe transcript levels of the class-II genes ... rrn23 ... were found to be decreased ... in the ... sig6 dg1 mutants
Chi W, Mao J, Li Q, Ji D, Zou M, Lu C, Zhang L - Interaction of the pentatricopeptide-repeat protein DELAYED GREENING 1 with sigma factor SIG6 in the regulation of chloroplast gene expression in Arabidopsis cotyledons
Visit the PubMed Articlethe transcript levels of the class-II ... rrn16 ... were found to be decreased ... in the ... sig6 ... mutant plants
Chi W, Mao J, Li Q, Ji D, Zou M, Lu C, Zhang L - Interaction of the pentatricopeptide-repeat protein DELAYED GREENING 1 with sigma factor SIG6 in the regulation of chloroplast gene expression in Arabidopsis cotyledons
Visit the PubMed Articlethe transcript levels of the class-II genes, ... rrn16 ... were found to be decreased ... in the ... sig6 dg1 mutants
Chi W, Mao J, Li Q, Ji D, Zou M, Lu C, Zhang L - Interaction of the pentatricopeptide-repeat protein DELAYED GREENING 1 with sigma factor SIG6 in the regulation of chloroplast gene expression in Arabidopsis cotyledons
Visit the PubMed ArticleAs shown in Figure 6d, the psbA transcript levels of the 35S:SIG6 dg1 lines were comparable with those of wild-type plants
Chi W, Mao J, Li Q, Ji D, Zou M, Lu C, Zhang L - Interaction of the pentatricopeptide-repeat protein DELAYED GREENING 1 with sigma factor SIG6 in the regulation of chloroplast gene expression in Arabidopsis cotyledons
Visit the PubMed ArticleOn the other hand, the transcript levels of NEP-dependent genes were obviously increased in dg1 mutants compared with wild-type plants (Figure 4); however, their run-on transcription activities were less changed
Chi W, Mao J, Li Q, Ji D, Zou M, Lu C, Zhang L - Interaction of the pentatricopeptide-repeat protein DELAYED GREENING 1 with sigma factor SIG6 in the regulation of chloroplast gene expression in Arabidopsis cotyledons
Visit the PubMed Articlethe transcript levels of the class-II genes ... rrn23 ... were found to be decreased ... in the dg1 ... mutant plants
Chi W, Mao J, Li Q, Ji D, Zou M, Lu C, Zhang L - Interaction of the pentatricopeptide-repeat protein DELAYED GREENING 1 with sigma factor SIG6 in the regulation of chloroplast gene expression in Arabidopsis cotyledons
Visit the PubMed ArticleExpression of either DG1 or SIG6 enhanced the transcript accumulation of the PEP-dependent genes in the dg1 protoplasts
Chi W, Mao J, Li Q, Ji D, Zou M, Lu C, Zhang L - Interaction of the pentatricopeptide-repeat protein DELAYED GREENING 1 with sigma factor SIG6 in the regulation of chloroplast gene expression in Arabidopsis cotyledons
Visit the PubMed ArticlepsbC levels of ... dg1 were reduced to about 20% of the wild-type levels
Chi W, Mao J, Li Q, Ji D, Zou M, Lu C, Zhang L - Interaction of the pentatricopeptide-repeat protein DELAYED GREENING 1 with sigma factor SIG6 in the regulation of chloroplast gene expression in Arabidopsis cotyledons
Visit the PubMed Articlethe transcript levels of the class-II ... rrn16 ... were found to be decreased ... in the dg1 ... mutant plants
Chi W, Mao J, Li Q, Ji D, Zou M, Lu C, Zhang L - Interaction of the pentatricopeptide-repeat protein DELAYED GREENING 1 with sigma factor SIG6 in the regulation of chloroplast gene expression in Arabidopsis cotyledons
Visit the PubMed ArticleNorthern blot analysis showed that the psbA transcript levels in sig6/SIG6 dg1 plants are about 20% and threefold of those of dg1 and double mutants, respectively
Chi W, Mao J, Li Q, Ji D, Zou M, Lu C, Zhang L - Interaction of the pentatricopeptide-repeat protein DELAYED GREENING 1 with sigma factor SIG6 in the regulation of chloroplast gene expression in Arabidopsis cotyledons
Visit the PubMed ArticlepYAB2:GUS ... expressed in the abaxial domain of lateral organs
Sarojam R, Sappl PG, Goldshmidt A, Efroni I, Floyd SK, Eshed Y, Bowman JL - Differentiating Arabidopsis shoots from leaves by combined YABBY activities
Visit the PubMed ArticleYAB5:GUS construct ... strongest expression in the ... midrib region
Sarojam R, Sappl PG, Goldshmidt A, Efroni I, Floyd SK, Eshed Y, Bowman JL - Differentiating Arabidopsis shoots from leaves by combined YABBY activities
Visit the PubMed ArticleYABBY triple mutants display reduced expression of ... AS2
Sarojam R, Sappl PG, Goldshmidt A, Efroni I, Floyd SK, Eshed Y, Bowman JL - Differentiating Arabidopsis shoots from leaves by combined YABBY activities
Visit the PubMed ArticlepYAB2:GUS construct was most pronounced in the ... midrib region
Sarojam R, Sappl PG, Goldshmidt A, Efroni I, Floyd SK, Eshed Y, Bowman JL - Differentiating Arabidopsis shoots from leaves by combined YABBY activities
Visit the PubMed ArticleYABBY triple mutants display reduced expression of ... ARF4
Sarojam R, Sappl PG, Goldshmidt A, Efroni I, Floyd SK, Eshed Y, Bowman JL - Differentiating Arabidopsis shoots from leaves by combined YABBY activities
Visit the PubMed ArticlepYAB5:GUS ... expressed in the abaxial domain of lateral organs
Sarojam R, Sappl PG, Goldshmidt A, Efroni I, Floyd SK, Eshed Y, Bowman JL - Differentiating Arabidopsis shoots from leaves by combined YABBY activities
Visit the PubMed ArticleYABBY triple mutants display reduced expression of ... REV
Sarojam R, Sappl PG, Goldshmidt A, Efroni I, Floyd SK, Eshed Y, Bowman JL - Differentiating Arabidopsis shoots from leaves by combined YABBY activities
Visit the PubMed ArticlepYAB5:GUS construct ... strongest expression in the petiole
Sarojam R, Sappl PG, Goldshmidt A, Efroni I, Floyd SK, Eshed Y, Bowman JL - Differentiating Arabidopsis shoots from leaves by combined YABBY activities
Visit the PubMed ArticleYABBY triple mutants display reduced expression of ... PHB
Sarojam R, Sappl PG, Goldshmidt A, Efroni I, Floyd SK, Eshed Y, Bowman JL - Differentiating Arabidopsis shoots from leaves by combined YABBY activities
Visit the PubMed Articleenhancer trap line (YJ158) that displays GUS activity in leaf margin cells ... in pANT>>miR-YAB13 leaves, GUS activity was weak, patchy, and mostly distal
Sarojam R, Sappl PG, Goldshmidt A, Efroni I, Floyd SK, Eshed Y, Bowman JL - Differentiating Arabidopsis shoots from leaves by combined YABBY activities
Visit the PubMed ArticlepYAB2:GUS construct was most pronounced in the petiole
Sarojam R, Sappl PG, Goldshmidt A, Efroni I, Floyd SK, Eshed Y, Bowman JL - Differentiating Arabidopsis shoots from leaves by combined YABBY activities
Visit the PubMed ArticleTo gain further insight into the function of TPS1 during normal development, we produced 24 independent transformants expressing the GUS reporter gene under the control of a 2 kb promoter region of the AtTPS1 gene. In all transformants, GUS expression during embryo development was similar to that reported by van Dijken et al. (2004) with staining concentrated in the meristematic region
Gómez LD, Gilday A, Feil R, Lunn JE, Graham IA - AtTPS1-mediated trehalose 6-phosphate synthesis is essential for embryogenic and vegetative growth and responsiveness to ABA in germinating seeds and stomatal guard cells
Visit the PubMed Articlewe found that transcript abundance of ... RAB18 ... increased in ABI3::TPS1 tps1/tps1 plantlets compared with wild-type plants
Gómez LD, Gilday A, Feil R, Lunn JE, Graham IA - AtTPS1-mediated trehalose 6-phosphate synthesis is essential for embryogenic and vegetative growth and responsiveness to ABA in germinating seeds and stomatal guard cells
Visit the PubMed ArticleTranscript levels of APL3, which encodes one of the four isoforms of the large subunit of ADP glucose pyrophosphorylase, a key enzyme in starch synthesis, are more than two times higher in ABI3::TPS1 tps1/tps1 plantlets compared with the wild type
Gómez LD, Gilday A, Feil R, Lunn JE, Graham IA - AtTPS1-mediated trehalose 6-phosphate synthesis is essential for embryogenic and vegetative growth and responsiveness to ABA in germinating seeds and stomatal guard cells
Visit the PubMed Articlewe found that transcript abundance of ... EM1 ... increased in ABI3::TPS1 tps1/tps1 plantlets compared with wild-type plants
Gómez LD, Gilday A, Feil R, Lunn JE, Graham IA - AtTPS1-mediated trehalose 6-phosphate synthesis is essential for embryogenic and vegetative growth and responsiveness to ABA in germinating seeds and stomatal guard cells
Visit the PubMed ArticleSUCROSE PHOSPHATE SYNTHASE (SPS) gene At4g10120 ... decreased in ABI3::TPS1 tps1/tps1 compared with wild-type plants of the same age
Gómez LD, Gilday A, Feil R, Lunn JE, Graham IA - AtTPS1-mediated trehalose 6-phosphate synthesis is essential for embryogenic and vegetative growth and responsiveness to ABA in germinating seeds and stomatal guard cells
Visit the PubMed ArticleTo gain further insight into the function of TPS1 during normal development, we produced 24 independent transformants expressing the GUS reporter gene under the control of a 2 kb promoter region of the AtTPS1 gene ... We also observed GUS expression in young seedlings with the first true leaves
Gómez LD, Gilday A, Feil R, Lunn JE, Graham IA - AtTPS1-mediated trehalose 6-phosphate synthesis is essential for embryogenic and vegetative growth and responsiveness to ABA in germinating seeds and stomatal guard cells
Visit the PubMed Articlewe found that transcript abundance of ABI4 ... increased in ABI3::TPS1 tps1/tps1 plantlets compared with wild-type plants
Gómez LD, Gilday A, Feil R, Lunn JE, Graham IA - AtTPS1-mediated trehalose 6-phosphate synthesis is essential for embryogenic and vegetative growth and responsiveness to ABA in germinating seeds and stomatal guard cells
Visit the PubMed Articlein ABI3::TPS1 tps1/tps1 plantlets ... transcript levels of SEX1, which encodes a protein involved in starch mobilisation (Yu et al., 2001), are marginally reduced
Gómez LD, Gilday A, Feil R, Lunn JE, Graham IA - AtTPS1-mediated trehalose 6-phosphate synthesis is essential for embryogenic and vegetative growth and responsiveness to ABA in germinating seeds and stomatal guard cells
Visit the PubMed ArticleTranscript levels of AKIN11, a component of the SnRK1 kinase, are decreased in ABI3::TPS1 tps1/tps1 plantlets
Gómez LD, Gilday A, Feil R, Lunn JE, Graham IA - AtTPS1-mediated trehalose 6-phosphate synthesis is essential for embryogenic and vegetative growth and responsiveness to ABA in germinating seeds and stomatal guard cells
Visit the PubMed Articlein ABI3::TPS1 tps1/tps1 plantlets ... AKIN10 ... similar to the wild type
Gómez LD, Gilday A, Feil R, Lunn JE, Graham IA - AtTPS1-mediated trehalose 6-phosphate synthesis is essential for embryogenic and vegetative growth and responsiveness to ABA in germinating seeds and stomatal guard cells
Visit the PubMed ArticleSUCROSE SYNTHASE1 (SUS1) gene At5g20830 ... decreased in ABI3::TPS1 tps1/tps1 compared with wild-type plants of the same age
Gómez LD, Gilday A, Feil R, Lunn JE, Graham IA - AtTPS1-mediated trehalose 6-phosphate synthesis is essential for embryogenic and vegetative growth and responsiveness to ABA in germinating seeds and stomatal guard cells
Visit the PubMed Articlein ABI3::TPS1 tps1/tps1 plantlets ... AKINβ ... similar to the wild type
Gómez LD, Gilday A, Feil R, Lunn JE, Graham IA - AtTPS1-mediated trehalose 6-phosphate synthesis is essential for embryogenic and vegetative growth and responsiveness to ABA in germinating seeds and stomatal guard cells
Visit the PubMed ArticleThe hexokinase protein plays a very important catalytic and signalling role in carbohydrate metabolism in Arabidopsis (Rolland et al., 2006), but we found only a 22% decrease in HXK1 transcripts in ABI3::TPS1 tps1/tps1 plantlets
Gómez LD, Gilday A, Feil R, Lunn JE, Graham IA - AtTPS1-mediated trehalose 6-phosphate synthesis is essential for embryogenic and vegetative growth and responsiveness to ABA in germinating seeds and stomatal guard cells
Visit the PubMed Articlein ABI3::TPS1 tps1/tps1 plantlets ... no change in those of HXK2 ... Transcript levels
Gómez LD, Gilday A, Feil R, Lunn JE, Graham IA - AtTPS1-mediated trehalose 6-phosphate synthesis is essential for embryogenic and vegetative growth and responsiveness to ABA in germinating seeds and stomatal guard cells
Visit the PubMed ArticleSTM ... transcripts were analyzed by mRNA in situ hybridization (Figure S2). The expression patterns of these genes did not differ significantly between the transgenic and wild-type plants, in either the CZ, PZ or RM, indicating no significant change in meristem organization ... PSTM:CDKA;1.N146 plants
Gaamouche T, Manes CL, Kwiatkowska D, Berckmans B, Koumproglou R, Maes S, Beeckman T, Vernoux T, Doonan JH, Traas J, Inzé D, De Veylder L - Cyclin-dependent kinase activity maintains the shoot apical meristem cells in an undifferentiated state
Visit the PubMed ArticleWUS ... transcripts were analyzed by mRNA in situ hybridization (Figure S2). The expression patterns of these genes did not differ significantly between the transgenic and wild-type plants, in either the CZ, PZ or RM, indicating no significant change in meristem organization ... PSTM:CDKA;1.N146 plants
Gaamouche T, Manes CL, Kwiatkowska D, Berckmans B, Koumproglou R, Maes S, Beeckman T, Vernoux T, Doonan JH, Traas J, Inzé D, De Veylder L - Cyclin-dependent kinase activity maintains the shoot apical meristem cells in an undifferentiated state
Visit the PubMed ArticleASYMMETRIC LEAVES 1 (AS1) transcripts were analyzed by mRNA in situ hybridization (Figure S2). The expression patterns of these genes did not differ significantly between the transgenic and wild-type plants, in either the CZ, PZ or RM, indicating no significant change in meristem organization ... PSTM:CDKA;1.N146 plants
Gaamouche T, Manes CL, Kwiatkowska D, Berckmans B, Koumproglou R, Maes S, Beeckman T, Vernoux T, Doonan JH, Traas J, Inzé D, De Veylder L - Cyclin-dependent kinase activity maintains the shoot apical meristem cells in an undifferentiated state
Visit the PubMed ArticleCLV3 ... transcripts were analyzed by mRNA in situ hybridization (Figure S2). The expression patterns of these genes did not differ significantly between the transgenic and wild-type plants, in either the CZ, PZ or RM, indicating no significant change in meristem organization ... PSTM:CDKA;1.N146 plants
Gaamouche T, Manes CL, Kwiatkowska D, Berckmans B, Koumproglou R, Maes S, Beeckman T, Vernoux T, Doonan JH, Traas J, Inzé D, De Veylder L - Cyclin-dependent kinase activity maintains the shoot apical meristem cells in an undifferentiated state
Visit the PubMed ArticleAtTCTPg-GFP plants, harboring the genomic sequence of AtTCTP containing the 5′ and 3′ UTRs, showed a strong GFP fluorescence in tissues undergoing active cell division, although low GFP fluorescence was observed in fully developed organs such as leaves or stems
Brioudes F, Thierry AM, Chambrier P, Mollereau B, Bendahmane M - Translationally controlled tumor protein is a conserved mitotic growth integrator in animals and plants
Visit the PubMed ArticleWe did not detect significant differences in the expression levels of the abaxial-promoting ... YAB5 ... in icu6/AXR3 or axr3-3/AXR3 mutant rosettes compared witho wild-type rosettes
Pérez-Pérez JM, Candela H, Robles P, López-Torrejón G, del Pozo JC, Micol JL - A role for AUXIN RESISTANT3 in the coordination of leaf growth
Visit the PubMed Articlethe expression levels of ... YAB3 ... were slightly decreased in icu6/AXR3
Pérez-Pérez JM, Candela H, Robles P, López-Torrejón G, del Pozo JC, Micol JL - A role for AUXIN RESISTANT3 in the coordination of leaf growth
Visit the PubMed ArticleSHY2 ... down-regulated in icu6/AXR3
Pérez-Pérez JM, Candela H, Robles P, López-Torrejón G, del Pozo JC, Micol JL - A role for AUXIN RESISTANT3 in the coordination of leaf growth
Visit the PubMed ArticleSHY2 ... down-regulated in axr1-12/axr1-12
Pérez-Pérez JM, Candela H, Robles P, López-Torrejón G, del Pozo JC, Micol JL - A role for AUXIN RESISTANT3 in the coordination of leaf growth
Visit the PubMed Articlethe expression levels of ... FILAMENTOUS FLOWER (FIL ... were slightly decreased in icu6/AXR3
Pérez-Pérez JM, Candela H, Robles P, López-Torrejón G, del Pozo JC, Micol JL - A role for AUXIN RESISTANT3 in the coordination of leaf growth
Visit the PubMed ArticleWe did not detect significant differences in the expression levels of the abaxial-promoting KAN1 ... in icu6/AXR3 or axr3-3/AXR3 mutant rosettes compared witho wild-type rosettes
Pérez-Pérez JM, Candela H, Robles P, López-Torrejón G, del Pozo JC, Micol JL - A role for AUXIN RESISTANT3 in the coordination of leaf growth
Visit the PubMed Articletranscript levels of ... AS2 remained unchanged in icu6/AXR3 rosettes compared with the wild type in qRT-PCR assays
Pérez-Pérez JM, Candela H, Robles P, López-Torrejón G, del Pozo JC, Micol JL - A role for AUXIN RESISTANT3 in the coordination of leaf growth
Visit the PubMed Articlea β-glucuronidase (GUS) reporter driven by a 2.0 kb genomic fragment upstream of AXR3 is expressed in leaf primordia
Pérez-Pérez JM, Candela H, Robles P, López-Torrejón G, del Pozo JC, Micol JL - A role for AUXIN RESISTANT3 in the coordination of leaf growth
Visit the PubMed ArticleAXR3 ... down-regulated in axr1-12/axr1-12
Pérez-Pérez JM, Candela H, Robles P, López-Torrejón G, del Pozo JC, Micol JL - A role for AUXIN RESISTANT3 in the coordination of leaf growth
Visit the PubMed ArticleIn contrast, the transcript levels of BP ... which are known targets of the AS1–AS2 pathway (Byrne et al. 2000, Byrne et al. 2002), were up-regulated in icu6/AXR3 rosettes
Pérez-Pérez JM, Candela H, Robles P, López-Torrejón G, del Pozo JC, Micol JL - A role for AUXIN RESISTANT3 in the coordination of leaf growth
Visit the PubMed ArticleWe next examined the expression pattern of pAS2::GUS, a reporter construct driven by the AS2 promoter (Iwakawa et al. 2007). As expected if ICU6 and AS2 act independently, similar patterns of GUS activity were detected in the adaxial domain of young cotyledons and leaves of wild-type plants (Fig. 6J, K) and in expanding leaves of icu6/AXR3 plants
Pérez-Pérez JM, Candela H, Robles P, López-Torrejón G, del Pozo JC, Micol JL - A role for AUXIN RESISTANT3 in the coordination of leaf growth
Visit the PubMed ArticleAXR2 ... down-regulated in icu6/AXR3
Pérez-Pérez JM, Candela H, Robles P, López-Torrejón G, del Pozo JC, Micol JL - A role for AUXIN RESISTANT3 in the coordination of leaf growth
Visit the PubMed ArticleWe did not detect significant differences in the expression levels of ... the adaxial-promoting PHABULOSA ... gene in icu6/AXR3 or axr3-3/AXR3 mutant rosettes compared witho wild-type rosettes
Pérez-Pérez JM, Candela H, Robles P, López-Torrejón G, del Pozo JC, Micol JL - A role for AUXIN RESISTANT3 in the coordination of leaf growth
Visit the PubMed ArticleIAA2 ... down-regulated in axr1-12/axr1-12
Pérez-Pérez JM, Candela H, Robles P, López-Torrejón G, del Pozo JC, Micol JL - A role for AUXIN RESISTANT3 in the coordination of leaf growth
Visit the PubMed Articletranscript levels of AS1 ... remained unchanged in icu6/AXR3 rosettes compared with the wild type in qRT-PCR assays
Pérez-Pérez JM, Candela H, Robles P, López-Torrejón G, del Pozo JC, Micol JL - A role for AUXIN RESISTANT3 in the coordination of leaf growth
Visit the PubMed ArticleAXR2 ... down-regulated in axr1-12/axr1-12
Pérez-Pérez JM, Candela H, Robles P, López-Torrejón G, del Pozo JC, Micol JL - A role for AUXIN RESISTANT3 in the coordination of leaf growth
Visit the PubMed ArticleIn contrast, the transcript levels of ... KNAT2, which are known targets of the AS1–AS2 pathway (Byrne et al. 2000, Byrne et al. 2002), were up-regulated in icu6/AXR3 rosettes
Pérez-Pérez JM, Candela H, Robles P, López-Torrejón G, del Pozo JC, Micol JL - A role for AUXIN RESISTANT3 in the coordination of leaf growth
Visit the PubMed ArticleSince ICS1 is responsible for pathogen-induced SA biosynthesis, the Atelp2 mutation may suppress the hyperaccumulation of SA in npr1 by inhibiting ICS1 expression. As shown in Figure 3a, Psm ES4326-induced ICS1 expression was significantly reduced in Atelp2 npr1 compared with that in npr1, indicating that AtELP2 is required for hyperaccumulation of ICS1 transcripts in npr1
DeFraia CT, Zhang X, Mou Z - Elongator subunit 2 is an accelerator of immune responses in Arabidopsis thaliana
Visit the PubMed Articlethe auxin-responsive genes IAA6 and IAA19 were decreased in Dof5.1-D
Kim HS, Kim SJ, Abbasi N, Bressan RA, Yun DJ, Yoo SD, Kwon SY, Choi SB - The DOF transcription factor Dof5.1 influences leaf axial patterning by promoting Revoluta transcription in Arabidopsis
Visit the PubMed Articlethe auxin-responsive genes IAA6 and IAA19 were decreased in Dof5.1-D
Kim HS, Kim SJ, Abbasi N, Bressan RA, Yun DJ, Yoo SD, Kwon SY, Choi SB - The DOF transcription factor Dof5.1 influences leaf axial patterning by promoting Revoluta transcription in Arabidopsis
Visit the PubMed ArticleREV and ATHB-15 trans-cripts were strongly enhanced in Dof5.1-D (Figure 6a); however, ATHB-15 was not increased in DEX::Dof5.1 plants upon DEX treatment whereas REV transcript was enhanced (Figure 6b). Increased expression of ATHB-15 in Dof5.1-D is probably due to a secondary effect
Kim HS, Kim SJ, Abbasi N, Bressan RA, Yun DJ, Yoo SD, Kwon SY, Choi SB - The DOF transcription factor Dof5.1 influences leaf axial patterning by promoting Revoluta transcription in Arabidopsis
Visit the PubMed ArticleA cross-section of GUS-stained rosette leaves and petioles showed that Dof5.1 was expressed highly in the vascular tissues
Kim HS, Kim SJ, Abbasi N, Bressan RA, Yun DJ, Yoo SD, Kwon SY, Choi SB - The DOF transcription factor Dof5.1 influences leaf axial patterning by promoting Revoluta transcription in Arabidopsis
Visit the PubMed ArticleREV expression was decreased in 35S::Dof5.1ΔAct plants although the levels were different depending on transgenic lines
Kim HS, Kim SJ, Abbasi N, Bressan RA, Yun DJ, Yoo SD, Kwon SY, Choi SB - The DOF transcription factor Dof5.1 influences leaf axial patterning by promoting Revoluta transcription in Arabidopsis
Visit the PubMed ArticleExpression of ... AtCLCb ... was almost undetectable, suggesting that mainly AtCLCa and AtCLCc are expressed in guard cells
Jossier M, Kroniewicz L, Dalmas F, Le Thiec D, Ephritikhine G, Thomine S, Barbier-Brygoo H, Vavasseur A, Filleur S, Leonhardt N - The Arabidopsis vacuolar anion transporter, AtCLCc, is involved in the regulation of stomatal movements and contributes to salt tolerance
Visit the PubMed ArticleTo determine the subcellular localization of AtCLCc, green fluorescent protein (GFP) was fused to its N-terminus, and the fusion protein was transiently expressed in protoplasts from Arabidopsis cell suspensions under the control of the CaMV 35S promoter. Confocal imaging showed that labelling by the GFP:AtCLCc fusion coincided with the tonoplast
Jossier M, Kroniewicz L, Dalmas F, Le Thiec D, Ephritikhine G, Thomine S, Barbier-Brygoo H, Vavasseur A, Filleur S, Leonhardt N - The Arabidopsis vacuolar anion transporter, AtCLCc, is involved in the regulation of stomatal movements and contributes to salt tolerance
Visit the PubMed ArticleTo confirm these results, quantitative reverse transcription PCR experiments were performed using RNAs prepared from highly purified guard cells and mesophyll cells from Arabidopsis plants treated or not with 100 μm ABA for 4 h. As shown in Figure 3(a), the expression level of AtCLCc was very high in guard cells compared to mesophyll cells
Jossier M, Kroniewicz L, Dalmas F, Le Thiec D, Ephritikhine G, Thomine S, Barbier-Brygoo H, Vavasseur A, Filleur S, Leonhardt N - The Arabidopsis vacuolar anion transporter, AtCLCc, is involved in the regulation of stomatal movements and contributes to salt tolerance
Visit the PubMed ArticleExpression of ... AtCLCg ... was almost undetectable, suggesting that mainly AtCLCa and AtCLCc are expressed in guard cells
Jossier M, Kroniewicz L, Dalmas F, Le Thiec D, Ephritikhine G, Thomine S, Barbier-Brygoo H, Vavasseur A, Filleur S, Leonhardt N - The Arabidopsis vacuolar anion transporter, AtCLCc, is involved in the regulation of stomatal movements and contributes to salt tolerance
Visit the PubMed ArticleIn addition, ABA treatment (10 μm ABA sprayed once at the start of the 4 h treatment) slightly increased the expression of AtCLCa
Jossier M, Kroniewicz L, Dalmas F, Le Thiec D, Ephritikhine G, Thomine S, Barbier-Brygoo H, Vavasseur A, Filleur S, Leonhardt N - The Arabidopsis vacuolar anion transporter, AtCLCc, is involved in the regulation of stomatal movements and contributes to salt tolerance
Visit the PubMed ArticleStudies of AtCLCc promoter activity (Figure 1) and analysis of the guard cell transcriptome (Leonhardt et al., 2004) revealed that AtCLCc is highly expressed in guard cells
Jossier M, Kroniewicz L, Dalmas F, Le Thiec D, Ephritikhine G, Thomine S, Barbier-Brygoo H, Vavasseur A, Filleur S, Leonhardt N - The Arabidopsis vacuolar anion transporter, AtCLCc, is involved in the regulation of stomatal movements and contributes to salt tolerance
Visit the PubMed ArticleExpression of ... AtCLCf ... was almost undetectable, suggesting that mainly AtCLCa and AtCLCc are expressed in guard cells
Jossier M, Kroniewicz L, Dalmas F, Le Thiec D, Ephritikhine G, Thomine S, Barbier-Brygoo H, Vavasseur A, Filleur S, Leonhardt N - The Arabidopsis vacuolar anion transporter, AtCLCc, is involved in the regulation of stomatal movements and contributes to salt tolerance
Visit the PubMed ArticleExposure of plants to light for 4 h resulted in dramatically decreased expression of AtCLCa
Jossier M, Kroniewicz L, Dalmas F, Le Thiec D, Ephritikhine G, Thomine S, Barbier-Brygoo H, Vavasseur A, Filleur S, Leonhardt N - The Arabidopsis vacuolar anion transporter, AtCLCc, is involved in the regulation of stomatal movements and contributes to salt tolerance
Visit the PubMed ArticleTo determine whether AtCLCc is involved in chloride homeostasis under salt stress, we measured the expression of AtCLCc in plants grown in a range of NaCl concentrations. Quantitative reverse transcription PCR experiments were performed on RNA extracted from leaves and roots of wild-type plants (Col-0 and WS) grown for 3 weeks in vitro on half-strength MS only or supplemented with 25 or 50 mm NaCl. AtCLCc expression was significantly increased in the leaves of both wild-type accessions (Figure 5a) in response to 25 and 50 mm NaCl in WS and 50 mm NaCl in Col-0. Induction was also observed in roots in response to 50 mm NaCl in the WS accession only
Jossier M, Kroniewicz L, Dalmas F, Le Thiec D, Ephritikhine G, Thomine S, Barbier-Brygoo H, Vavasseur A, Filleur S, Leonhardt N - The Arabidopsis vacuolar anion transporter, AtCLCc, is involved in the regulation of stomatal movements and contributes to salt tolerance
Visit the PubMed ArticleExposure of plants to light for 4 h resulted in dramatically decreased expression of ... AtCLCc in guard cells
Jossier M, Kroniewicz L, Dalmas F, Le Thiec D, Ephritikhine G, Thomine S, Barbier-Brygoo H, Vavasseur A, Filleur S, Leonhardt N - The Arabidopsis vacuolar anion transporter, AtCLCc, is involved in the regulation of stomatal movements and contributes to salt tolerance
Visit the PubMed ArticleExpression of ... AtCLCe ... was almost undetectable, suggesting that mainly AtCLCa and AtCLCc are expressed in guard cells
Jossier M, Kroniewicz L, Dalmas F, Le Thiec D, Ephritikhine G, Thomine S, Barbier-Brygoo H, Vavasseur A, Filleur S, Leonhardt N - The Arabidopsis vacuolar anion transporter, AtCLCc, is involved in the regulation of stomatal movements and contributes to salt tolerance
Visit the PubMed ArticleA genomic region comprising 1437 bp of the AtCLCc promoter upstream of the start codon was fused in-frame with the uidA reporter gene, and introduced into Arabidopsis wild-type plants. All T3 plants obtained from eight independent AtCLCc::GUS transgenic lines showed a similar qualitative pattern of uidA expression that corresponded to the quantitative reverse transcription PCR data
Jossier M, Kroniewicz L, Dalmas F, Le Thiec D, Ephritikhine G, Thomine S, Barbier-Brygoo H, Vavasseur A, Filleur S, Leonhardt N - The Arabidopsis vacuolar anion transporter, AtCLCc, is involved in the regulation of stomatal movements and contributes to salt tolerance
Visit the PubMed ArticleExpression of ... AtCLCd ... was almost undetectable, suggesting that mainly AtCLCa and AtCLCc are expressed in guard cells
Jossier M, Kroniewicz L, Dalmas F, Le Thiec D, Ephritikhine G, Thomine S, Barbier-Brygoo H, Vavasseur A, Filleur S, Leonhardt N - The Arabidopsis vacuolar anion transporter, AtCLCc, is involved in the regulation of stomatal movements and contributes to salt tolerance
Visit the PubMed ArticleThe expression level of AtCLCc mRNAs was determined in leaves, flowers and pollen from soil-grown plants and germinated seeds and roots from in vitro cultures. Quantitative reverse transcription PCR showed transcript accumulation mainly in aerial organs, with maximal amounts in pollen
Jossier M, Kroniewicz L, Dalmas F, Le Thiec D, Ephritikhine G, Thomine S, Barbier-Brygoo H, Vavasseur A, Filleur S, Leonhardt N - The Arabidopsis vacuolar anion transporter, AtCLCc, is involved in the regulation of stomatal movements and contributes to salt tolerance
Visit the PubMed ArticleABA treatment led to a significant increase in AtCLCc expression in guard cells
Jossier M, Kroniewicz L, Dalmas F, Le Thiec D, Ephritikhine G, Thomine S, Barbier-Brygoo H, Vavasseur A, Filleur S, Leonhardt N - The Arabidopsis vacuolar anion transporter, AtCLCc, is involved in the regulation of stomatal movements and contributes to salt tolerance
Visit the PubMed ArticleTo determine the subcellular localization of AtCLCc, green fluorescent protein (GFP) was fused to its N-terminus ... Stable transformation of A. thaliana with the same construct confirmed tonoplast localization in stomata
Jossier M, Kroniewicz L, Dalmas F, Le Thiec D, Ephritikhine G, Thomine S, Barbier-Brygoo H, Vavasseur A, Filleur S, Leonhardt N - The Arabidopsis vacuolar anion transporter, AtCLCc, is involved in the regulation of stomatal movements and contributes to salt tolerance
Visit the PubMed ArticleTo gain an insight into whether the RAV genes are also involved in pathways common to plant defence and senescence, the expression patterns of RAV1, TEM1, and RAV3 were examined in response to the defence-associated phytohormones, ethylene and MJ ... The transcript levels for the three RAV genes also rapidly increased in response to MJ (Fig. 3C). Twenty hours after MJ treatment, RAV1 transcripts remained at higher levels, but the TEM1 and RAV3 transcripts decreased. These results indicate that the RAV genes might play an important role during senescence modulated by ethylene and MJ as well as during age-dependent senescence
Woo HR, Kim JH, Kim J, Kim J, Lee U, Song IJ, Kim JH, Lee HY, Nam HG, Lim PO - The RAV1 transcription factor positively regulates leaf senescence in Arabidopsis
Visit the PubMed ArticleTo gain an insight into whether the RAV genes are also involved in pathways common to plant defence and senescence, the expression patterns of RAV1, TEM1, and RAV3 were examined in response to the defence-associated phytohormones, ethylene and MJ. As shown in Fig. 3, the transcript levels of these three genes were regulated in a similar manner. Two hours after treatment with ACC, the ethylene precursor, the expression of the three RAV genes was strongly triggered but declined again 20 h afterwards
Woo HR, Kim JH, Kim J, Kim J, Lee U, Song IJ, Kim JH, Lee HY, Nam HG, Lim PO - The RAV1 transcription factor positively regulates leaf senescence in Arabidopsis
Visit the PubMed ArticleTo gain an insight into whether the RAV genes are also involved in pathways common to plant defence and senescence, the expression patterns of RAV1, TEM1, and RAV3 were examined in response to the defence-associated phytohormones, ethylene and MJ. As shown in Fig. 3, the transcript levels of these three genes were regulated in a similar manner. Two hours after treatment with ACC, the ethylene precursor, the expression of the three RAV genes was strongly triggered but declined again 20 h afterwards
Woo HR, Kim JH, Kim J, Kim J, Lee U, Song IJ, Kim JH, Lee HY, Nam HG, Lim PO - The RAV1 transcription factor positively regulates leaf senescence in Arabidopsis
Visit the PubMed ArticleThe expression of the ... senescence molecular markers ... SAG12 (Fig. 4E) was investigated further ... However, in RAV1-overexpressing lines ... SAG12 transcripts started to accumulate in leaves at 22 and 16–18 DAE in wild type and overexpressors, respectively
Woo HR, Kim JH, Kim J, Kim J, Lee U, Song IJ, Kim JH, Lee HY, Nam HG, Lim PO - The RAV1 transcription factor positively regulates leaf senescence in Arabidopsis
Visit the PubMed ArticleTo determine the subcellular localization of RAV1, a RAV1-GFP (green fluorescent protein) fusion protein was expressed under the control of the 35S promoter in Arabidopsis protoplasts. Colocalization with a known nuclear protein, RFP-SHY2 (red fluorescent protein-suppressor of HY2), revealed that RAV1-GFP selectively localized to the nucleus (Fig. 2B), further implying that RAV1 functions as a transcriptional regulator
Woo HR, Kim JH, Kim J, Kim J, Lee U, Song IJ, Kim JH, Lee HY, Nam HG, Lim PO - The RAV1 transcription factor positively regulates leaf senescence in Arabidopsis
Visit the PubMed ArticleTo gain an insight into whether the RAV genes are also involved in pathways common to plant defence and senescence, the expression patterns of RAV1, TEM1, and RAV3 were examined in response to the defence-associated phytohormones, ethylene and MJ. As shown in Fig. 3, the transcript levels of these three genes were regulated in a similar manner. Two hours after treatment with ACC, the ethylene precursor, the expression of the three RAV genes was strongly triggered but declined again 20 h afterwards
Woo HR, Kim JH, Kim J, Kim J, Lee U, Song IJ, Kim JH, Lee HY, Nam HG, Lim PO - The RAV1 transcription factor positively regulates leaf senescence in Arabidopsis
Visit the PubMed ArticleThe RAV1 expression pattern during leaf development was first analysed in detail in the third and fourth rosette leaves at different ages by RNA gel blot analysis ... The RAV1 transcript ... began to increase at the late mature green stage, reaching a maximum level at the early senescence stage, but decreasing again when the SEN4 and SAG12 transcript levels markedly increased. The RAV1 expression profile during leaf development indicated that RAV1 might play a role in regulating the onset of leaf senescence
Woo HR, Kim JH, Kim J, Kim J, Lee U, Song IJ, Kim JH, Lee HY, Nam HG, Lim PO - The RAV1 transcription factor positively regulates leaf senescence in Arabidopsis
Visit the PubMed ArticleThe expression of the ... senescence molecular markers ... SEN4 ... was investigated further. In wild-type leaves ... However, in RAV1-overexpressing lines ... The expression of SEN4 was detectable at 22 DAE and 16 DAE in the wild type and overexpressors, respectively
Woo HR, Kim JH, Kim J, Kim J, Lee U, Song IJ, Kim JH, Lee HY, Nam HG, Lim PO - The RAV1 transcription factor positively regulates leaf senescence in Arabidopsis
Visit the PubMed ArticleThe expression of the photosynthesis-related gene CAB2 ... was investigated further. In wild-type leaves, the CAB2 transcript was highly expressed until 20 DAE. However, in RAV1-overexpressing lines, CAB2 expression was greatly reduced at 18 DAE
Woo HR, Kim JH, Kim J, Kim J, Lee U, Song IJ, Kim JH, Lee HY, Nam HG, Lim PO - The RAV1 transcription factor positively regulates leaf senescence in Arabidopsis
Visit the PubMed ArticleThe RAV1 expression pattern during leaf development was first analysed in detail in the third and fourth rosette leaves at different ages by RNA gel blot analysis ... The RAV1 transcript began to increase at the late mature green stage, reaching a maximum level at the early senescence stage
Woo HR, Kim JH, Kim J, Kim J, Lee U, Song IJ, Kim JH, Lee HY, Nam HG, Lim PO - The RAV1 transcription factor positively regulates leaf senescence in Arabidopsis
Visit the PubMed ArticleTo gain an insight into whether the RAV genes are also involved in pathways common to plant defence and senescence, the expression patterns of RAV1, TEM1, and RAV3 were examined in response to the defence-associated phytohormones, ethylene and MJ ... The transcript levels for the three RAV genes also rapidly increased in response to MJ (Fig. 3C). Twenty hours after MJ treatment, RAV1 transcripts remained at higher levels, but the TEM1 and RAV3 transcripts decreased. These results indicate that the RAV genes might play an important role during senescence modulated by ethylene and MJ as well as during age-dependent senescence
Woo HR, Kim JH, Kim J, Kim J, Lee U, Song IJ, Kim JH, Lee HY, Nam HG, Lim PO - The RAV1 transcription factor positively regulates leaf senescence in Arabidopsis
Visit the PubMed ArticleTo gain an insight into whether the RAV genes are also involved in pathways common to plant defence and senescence, the expression patterns of RAV1, TEM1, and RAV3 were examined in response to the defence-associated phytohormones, ethylene and MJ ... The transcript levels for the three RAV genes also rapidly increased in response to MJ (Fig. 3C). Twenty hours after MJ treatment, RAV1 transcripts remained at higher levels, but the TEM1 and RAV3 transcripts decreased. These results indicate that the RAV genes might play an important role during senescence modulated by ethylene and MJ as well as during age-dependent senescence
Woo HR, Kim JH, Kim J, Kim J, Lee U, Song IJ, Kim JH, Lee HY, Nam HG, Lim PO - The RAV1 transcription factor positively regulates leaf senescence in Arabidopsis
Visit the PubMed Articlesemiquantitative RT-PCR analyses on ... EXO70 genes in Arabidopsis ... RNA samples ... prepared from ... rosette leaves ... genes showed expression in different tissues (Fig. 1
Li S, van Os GM, Ren S, Yu D, Ketelaar T, Emons AM, Liu CM - Expression and functional analyses of EXO70 genes in Arabidopsis implicate their roles in regulating cell type-specific exocytosis
Visit the PubMed ArticleGUS expression in ... EXO70A2 ... were expressed in developing guard cells but not in mature ones
Li S, van Os GM, Ren S, Yu D, Ketelaar T, Emons AM, Liu CM - Expression and functional analyses of EXO70 genes in Arabidopsis implicate their roles in regulating cell type-specific exocytosis
Visit the PubMed Articlesemiquantitative RT-PCR analyses on ... EXO70 genes in Arabidopsis ... RNA samples ... prepared from ... rosette leaves ... genes showed expression in different tissues (Fig. 1 ... EXO70B2
Li S, van Os GM, Ren S, Yu D, Ketelaar T, Emons AM, Liu CM - Expression and functional analyses of EXO70 genes in Arabidopsis implicate their roles in regulating cell type-specific exocytosis
Visit the PubMed Articlesemiquantitative RT-PCR analyses on ... EXO70 genes in Arabidopsis ... RNA samples ... prepared from ... rosette leaves ... genes showed expression in different tissues (Fig. 1
Li S, van Os GM, Ren S, Yu D, Ketelaar T, Emons AM, Liu CM - Expression and functional analyses of EXO70 genes in Arabidopsis implicate their roles in regulating cell type-specific exocytosis
Visit the PubMed ArticleEXO70D3 ... GUS expression became detectable when the trichomes started to form, and ... was absent from mature trichomes
Li S, van Os GM, Ren S, Yu D, Ketelaar T, Emons AM, Liu CM - Expression and functional analyses of EXO70 genes in Arabidopsis implicate their roles in regulating cell type-specific exocytosis
Visit the PubMed Articlefusion constructs carrying 5′ upstream sequences fused to the coding region of the GUS gene ... EXO70A1 ... we found a xylem-specific expression ... of Arabidopsis ... leaves ... restricted to the stage when banded cell wall thickenings are being formed, whereas no GUS expression was observed before and after this stage
Li S, van Os GM, Ren S, Yu D, Ketelaar T, Emons AM, Liu CM - Expression and functional analyses of EXO70 genes in Arabidopsis implicate their roles in regulating cell type-specific exocytosis
Visit the PubMed ArticleGUS expression in ... EXO70C1 ... were expressed in developing guard cells but not in mature ones
Li S, van Os GM, Ren S, Yu D, Ketelaar T, Emons AM, Liu CM - Expression and functional analyses of EXO70 genes in Arabidopsis implicate their roles in regulating cell type-specific exocytosis
Visit the PubMed Articlesemiquantitative RT-PCR analyses ... on ... EXO70 genes in Arabidopsis ... RNA samples ... prepared from ... rosette leaves ... genes showed expression in different tissues (Fig. 1 ... EXO70A1
Li S, van Os GM, Ren S, Yu D, Ketelaar T, Emons AM, Liu CM - Expression and functional analyses of EXO70 genes in Arabidopsis implicate their roles in regulating cell type-specific exocytosis
Visit the PubMed Articlesemiquantitative RT-PCR analyses on ... EXO70 genes in Arabidopsis ... RNA samples ... prepared from ... rosette leaves ... genes showed expression in different tissues (Fig. 1 ... EXO70D2
Li S, van Os GM, Ren S, Yu D, Ketelaar T, Emons AM, Liu CM - Expression and functional analyses of EXO70 genes in Arabidopsis implicate their roles in regulating cell type-specific exocytosis
Visit the PubMed Articlexylem-specific expression ... EXO70G2 ... were expressed in developing xylem elements during secondary cell wall thickening ... as revealed by GUS assays
Li S, van Os GM, Ren S, Yu D, Ketelaar T, Emons AM, Liu CM - Expression and functional analyses of EXO70 genes in Arabidopsis implicate their roles in regulating cell type-specific exocytosis
Visit the PubMed Articlesemiquantitative RT-PCR analyses on ... EXO70 genes in Arabidopsis ... RNA samples ... prepared from ... rosette leaves ... genes showed expression in different tissues (Fig. 1 ... EXO70B1
Li S, van Os GM, Ren S, Yu D, Ketelaar T, Emons AM, Liu CM - Expression and functional analyses of EXO70 genes in Arabidopsis implicate their roles in regulating cell type-specific exocytosis
Visit the PubMed ArticleEXO70D2 ... GUS expression became detectable when the trichomes started to form, and ... was absent from mature trichomes
Li S, van Os GM, Ren S, Yu D, Ketelaar T, Emons AM, Liu CM - Expression and functional analyses of EXO70 genes in Arabidopsis implicate their roles in regulating cell type-specific exocytosis
Visit the PubMed ArticleGUS expression in ... EXO70H4 ... were expressed in developing guard cells but not in mature ones
Li S, van Os GM, Ren S, Yu D, Ketelaar T, Emons AM, Liu CM - Expression and functional analyses of EXO70 genes in Arabidopsis implicate their roles in regulating cell type-specific exocytosis
Visit the PubMed Articlesemiquantitative RT-PCR analyses on ... EXO70 genes in Arabidopsis ... RNA samples ... prepared from ... rosette leaves ... genes showed expression in different tissues (Fig. 1 ... EXO70G1
Li S, van Os GM, Ren S, Yu D, Ketelaar T, Emons AM, Liu CM - Expression and functional analyses of EXO70 genes in Arabidopsis implicate their roles in regulating cell type-specific exocytosis
Visit the PubMed Articlesemiquantitative RT-PCR analyses on ... EXO70 genes in Arabidopsis ... RNA samples ... prepared from ... rosette leaves ... genes showed expression in different tissues (Fig. 1 ... EXO70H7
Li S, van Os GM, Ren S, Yu D, Ketelaar T, Emons AM, Liu CM - Expression and functional analyses of EXO70 genes in Arabidopsis implicate their roles in regulating cell type-specific exocytosis
Visit the PubMed Articlesemiquantitative RT-PCR analyses on ... EXO70 genes in Arabidopsis ... RNA samples ... prepared from ... rosette leaves ... genes showed expression in different tissues (Fig. 1
Li S, van Os GM, Ren S, Yu D, Ketelaar T, Emons AM, Liu CM - Expression and functional analyses of EXO70 genes in Arabidopsis implicate their roles in regulating cell type-specific exocytosis
Visit the PubMed Articlesemiquantitative RT-PCR analyses on ... EXO70 genes in Arabidopsis ... RNA samples ... prepared from ... rosette leaves ... genes showed expression in different tissues (Fig. 1
Li S, van Os GM, Ren S, Yu D, Ketelaar T, Emons AM, Liu CM - Expression and functional analyses of EXO70 genes in Arabidopsis implicate their roles in regulating cell type-specific exocytosis
Visit the PubMed Articlesemiquantitative RT-PCR analyses on ... EXO70 genes in Arabidopsis ... RNA samples ... prepared from ... rosette leaves ... genes showed expression in different tissues (Fig. 1
Li S, van Os GM, Ren S, Yu D, Ketelaar T, Emons AM, Liu CM - Expression and functional analyses of EXO70 genes in Arabidopsis implicate their roles in regulating cell type-specific exocytosis
Visit the PubMed Articlexylem-specific expression ... restricted to the stage when banded cell wall thickenings are being formed ... expression specificity of EXO70A1 was confirmed by RNA in situ hybridization
Li S, van Os GM, Ren S, Yu D, Ketelaar T, Emons AM, Liu CM - Expression and functional analyses of EXO70 genes in Arabidopsis implicate their roles in regulating cell type-specific exocytosis
Visit the PubMed ArticleThe expression pattern of miR396 was further investigated using an antisense LNA-modified DNA probe, which could detect the mature products of both MIR396a and MIR396b. As shown in Fig. 5E, the LNA signals were detected in the SAM and leaf primordia, and appeared to have a higher level in the distal part of primoridia, but no apparent polar distribution along the adaxial–abaxial axis was observed
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleTo analyse the temporal–spatial regulation of cell division by miR396–AtGRF modules, the expression patterns of AtGRF2, AtGRF9, and AtGIF1/AN3 were first examined by in situ hybridization. AtGRF2 and AtGRF9 were expressed in the SAM and young leaf primordium
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleqRT-PCR analysis revealed that the transcription levels of the nine AtGRF genes including seven predicated AtGRF gene targets in 35S:miR396a and 35S:miR396b plants were all decreased
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleIn addition to histone H4, the expression of several other cell cycle-related marker genes was also analysed by qRT-PCR. Cyclin D3;1 (CycD3;1) is mainly expressed at proliferating cells in the G1 phase and drives the G1/S transition together with cyclin-dependent kinase A (CDKA) (Dewitte et al., 2003; Menges et al., 2006). CYCA2;1 is involved in S/G2 transition, and CYCB1;1 in G2/M transition (Doerner et al., 1996; Shaul et al., 1996 ... the mRNA levels of cell cycle marker genes were reduced even more (5- to 15-fold change) in 35S:miR396a/as2 plants (Fig. S2D) as compared with those in the as2 mutant (Fig. S2A, D
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleIn contrast, FIL was expressed throughout the entire primordium of the needle-like leaves of 35S:miR396a/as2 plants and seemed to accumulate to higher levels in the other leaves (Fig. 3L
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleqRT-PCR analysis revealed that the transcription levels of the nine AtGRF genes including seven predicated AtGRF gene targets in 35S:miR396a and 35S:miR396b plants were all decreased
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleIn addition to histone H4, the expression of several other cell cycle-related marker genes was also analysed by qRT-PCR. Cyclin D3;1 (CycD3;1) is mainly expressed at proliferating cells in the G1 phase and drives the G1/S transition together with cyclin-dependent kinase A (CDKA) (Dewitte et al., 2003; Menges et al., 2006). CYCA2;1 is involved in S/G2 transition, and CYCB1;1 in G2/M transition (Doerner et al., 1996; Shaul et al., 1996 ... the mRNA levels of cell cycle marker genes were reduced even more (5- to 15-fold change) in 35S:miR396a/as2 plants (Fig. S2D) as compared with those in the as2 mutant (Fig. S2A, D
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleqRT-PCR analysis revealed that the transcription levels of the nine AtGRF genes including seven predicated AtGRF gene targets in 35S:miR396a and 35S:miR396b plants were all decreased
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleqRT-PCR analysis revealed that the transcription levels of the nine AtGRF genes including seven predicated AtGRF gene targets in 35S:miR396a and 35S:miR396b plants were all decreased
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleCompared with the wild type (Fig. 4A), the number of leaf cells expressing histone H4 was dramatically reduced in the 35S:miR396a plants
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleTo obtain molecular evidence that miR396-targeted AtGRFs are required for establishment of leaf polarity, the expression pattern of a YABBY family gene, FIL/YAB1, was examined by in situ hybridization. FIL is usually expressed on the leaf abaxial domain of both the wild type (Fig. 3J) and the as2 mutant (Fig. 3K
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleAlong the adaxial–abaxial axis, they were expressed uniformly throughout the young leaf primordia, similar to the pattern of AS1 (Fig. 5D), which is believed to be expressed in the whole primordium
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleIn addition to histone H4, the expression of several other cell cycle-related marker genes was also analysed by qRT-PCR. Cyclin D3;1 (CycD3;1) is mainly expressed at proliferating cells in the G1 phase and drives the G1/S transition together with cyclin-dependent kinase A (CDKA) (Dewitte et al., 2003; Menges et al., 2006). CYCA2;1 is involved in S/G2 transition, and CYCB1;1 in G2/M transition (Doerner et al., 1996; Shaul et al., 1996). The expression of all the tested marker genes was reduced in 35S:miR396a plants with a 2- to 4-fold change
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleqRT-PCR analysis revealed that the transcription levels of the nine AtGRF genes including seven predicated AtGRF gene targets in 35S:miR396a and 35S:miR396b plants were all decreased
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleqRT-PCR analysis revealed that the transcription levels of the nine AtGRF genes including seven predicated AtGRF gene targets in 35S:miR396a and 35S:miR396b plants were all decreased
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleTo analyse the temporal–spatial regulation of cell division by miR396–AtGRF modules, the expression patterns of AtGRF2, AtGRF9, and AtGIF1/AN3 were first examined by in situ hybridization. AtGRF2 and AtGRF9 were expressed in the SAM and young leaf primordium
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed Articlefunctional domain of the promoters of MIR396A and MIR396B in the leaves was therefore characterized to better understand the expression pattern of miR396. The ∼3.2 kb 5' upstream regions of MIR396A and MIR396B (counting from the first nucleotide of the mature miR396 sequences) were cloned to represent their respectiveputative promoters. The fragments were fused with the GUS reporter gene to generate the p396a:GUS and p396b:GUS constructs (Fig. 5H), which were then introduced into wild-type plants. By examining the GUS staining signals, it was found that dynamic but similar patterns for both constructs were detected from the cotyledon, shoot apex, and rosette leaves ... In young leaves, the GUS signals were strong in the tip and distal parts, opposite to the expression pattern of CYCB1;1:GUS
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleqRT-PCR analysis revealed that the transcription levels of the nine AtGRF genes including seven predicated AtGRF gene targets in 35S:miR396a and 35S:miR396b plants were all decreased
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleqRT-PCR analysis revealed that the transcription levels of the nine AtGRF genes including seven predicated AtGRF gene targets in 35S:miR396a and 35S:miR396b plants were all decreased
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed Articlefunctional domain of the promoters of MIR396A and MIR396B in the leaves was therefore characterized to better understand the expression pattern of miR396. The ∼3.2 kb 5' upstream regions of MIR396A and MIR396B (counting from the first nucleotide of the mature miR396 sequences) were cloned to represent their respectiveputative promoters. The fragments were fused with the GUS reporter gene to generate the p396a:GUS and p396b:GUS constructs (Fig. 5H), which were then introduced into wild-type plants. By examining the GUS staining signals, it was found that dynamic but similar patterns for both constructs were detected from the cotyledon, shoot apex, and rosette leaves ... In the cotyledon, the GUS signals were very strong (Fig. 5I–J), in contrast to almost no signals of CYCB1;1:GUS
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleqRT-PCR analysis revealed that the transcription levels of the nine AtGRF genes including seven predicated AtGRF gene targets in 35S:miR396a and 35S:miR396b plants were all decreased
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleqRT-PCR analysis revealed that the transcription levels of the nine AtGRF genes including seven predicated AtGRF gene targets in 35S:miR396a and 35S:miR396b plants were all decreased
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleIn addition to histone H4, the expression of several other cell cycle-related marker genes was also analysed by qRT-PCR. Cyclin D3;1 (CycD3;1) is mainly expressed at proliferating cells in the G1 phase and drives the G1/S transition together with cyclin-dependent kinase A (CDKA) (Dewitte et al., 2003; Menges et al., 2006). CYCA2;1 is involved in S/G2 transition, and CYCB1;1 in G2/M transition (Doerner et al., 1996; Shaul et al., 1996 ... the mRNA levels of cell cycle marker genes were reduced even more (5- to 15-fold change) in 35S:miR396a/as2 plants (Fig. S2D) as compared with those in the as2 mutant (Fig. S2A, D
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleqRT-PCR analysis revealed that the transcription levels of the nine AtGRF genes including seven predicated AtGRF gene targets in 35S:miR396a and 35S:miR396b plants were all decreased
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed Articlefunctional domain of the promoters of MIR396A and MIR396B in the leaves was therefore characterized to better understand the expression pattern of miR396. The ∼3.2 kb 5' upstream regions of MIR396A and MIR396B (counting from the first nucleotide of the mature miR396 sequences) were cloned to represent their respectiveputative promoters. The fragments were fused with the GUS reporter gene to generate the p396a:GUS and p396b:GUS constructs (Fig. 5H), which were then introduced into wild-type plants. By examining the GUS staining signals, it was found that dynamic but similar patterns for both constructs were detected from the cotyledon, shoot apex, and rosette leaves ... In young leaves, the GUS signals were strong in the tip and distal parts, opposite to the expression pattern of CYCB1;1:GUS
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleThe transcription levels of AtGRF genes were analysed in 35S:miR396a/as2 transgenic plants by qRT-PCR. Similar to 35S:miR396/Col transgenic plants, the expression levels of AtGRF genes were decreased in 35S:miR396a/as2 (Fig. 1J), suggesting that the leaf polarity defects in 35S:miR396a/as2 plants were due to the decreased expression of AtGRF genes
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleThe expression pattern of miR396 was further investigated using an antisense LNA-modified DNA probe, which could detect the mature products of both MIR396a and MIR396b. As shown in Fig. 5E, the LNA signals were detected in the SAM and leaf primordia, and appeared to have a higher level in the distal part of primoridia, but no apparent polar distribution along the adaxial–abaxial axis was observed
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleqRT-PCR analysis revealed that the transcription levels of the nine AtGRF genes including seven predicated AtGRF gene targets in 35S:miR396a and 35S:miR396b plants were all decreased
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed Articlefunctional domain of the promoters of MIR396A and MIR396B in the leaves was therefore characterized to better understand the expression pattern of miR396. The ∼3.2 kb 5' upstream regions of MIR396A and MIR396B (counting from the first nucleotide of the mature miR396 sequences) were cloned to represent their respectiveputative promoters. The fragments were fused with the GUS reporter gene to generate the p396a:GUS and p396b:GUS constructs (Fig. 5H), which were then introduced into wild-type plants. By examining the GUS staining signals, it was found that dynamic but similar patterns for both constructs were detected from the cotyledon, shoot apex, and rosette leaves ... ( ... In young leaves, the GUS signals were strong in the tip and distal parts, opposite to the expression pattern of CYCB1;1:GUS
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleqRT-PCR analysis revealed that the transcription levels of the nine AtGRF genes including seven predicated AtGRF gene targets in 35S:miR396a and 35S:miR396b plants were all decreased
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed Articlefunctional domain of the promoters of MIR396A and MIR396B in the leaves was therefore characterized to better understand the expression pattern of miR396. The ∼3.2 kb 5' upstream regions of MIR396A and MIR396B (counting from the first nucleotide of the mature miR396 sequences) were cloned to represent their respectiveputative promoters. The fragments were fused with the GUS reporter gene to generate the p396a:GUS and p396b:GUS constructs (Fig. 5H), which were then introduced into wild-type plants. By examining the GUS staining signals, it was found that dynamic but similar patterns for both constructs were detected from the cotyledon, shoot apex, and rosette leaves ... In the cotyledon, the GUS signals were very strong (Fig. 5I–J), in contrast to almost no signals of CYCB1;1:GUS
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleqRT-PCR analysis revealed that the transcription levels of the nine AtGRF genes including seven predicated AtGRF gene targets in 35S:miR396a and 35S:miR396b plants were all decreased
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleTo analyse the temporal–spatial regulation of cell division by miR396–AtGRF modules, the expression patterns of AtGRF2, AtGRF9, and AtGIF1/AN3 were first examined by in situ hybridization. AtGRF2 and AtGRF9 were expressed in the SAM and young leaf primordium
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleqRT-PCR analysis revealed that the transcription levels of the nine AtGRF genes including seven predicated AtGRF gene targets in 35S:miR396a and 35S:miR396b plants were all decreased
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleThe transcription levels of AtGRF genes were analysed in 35S:miR396a/as2 transgenic plants by qRT-PCR. Similar to 35S:miR396/Col transgenic plants, the expression levels of AtGRF genes were decreased in 35S:miR396a/as2 (Fig. 1J), suggesting that the leaf polarity defects in 35S:miR396a/as2 plants were due to the decreased expression of AtGRF genes
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed Articlefunctional domain of the promoters of MIR396A and MIR396B in the leaves was therefore characterized to better understand the expression pattern of miR396. The ∼3.2 kb 5' upstream regions of MIR396A and MIR396B (counting from the first nucleotide of the mature miR396 sequences) were cloned to represent their respectiveputative promoters. The fragments were fused with the GUS reporter gene to generate the p396a:GUS and p396b:GUS constructs (Fig. 5H), which were then introduced into wild-type plants. By examining the GUS staining signals, it was found that dynamic but similar patterns for both constructs were detected from the cotyledon, shoot apex, and rosette leaves ... However, when the leaves gradually become mature, the GUS signals progressively accumulate from the distal to the proximal part of developing leaves, with strong activity in the whole mature leaves
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleTo confirm this further, the construct 35S:rAtGRF9, which carries the miR396-resistant version of AtGRF9 (rAtGRF9), was made and introduced into 35S:miR396a/as2 plants ... Disruption of the miR396-binding sites in AtGRF9 could result in the accumulation of their stabilized transcripts in transgenic plants. By qRT-PCR, it was found that the AtGRF9 mRNA in the double transgenic plants was increased to levels higher than in the wild type, in contrast to the much lower accumulation in 35S:miR396a/as2 plants
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleThe expression pattern of miR396 was further investigated using an antisense LNA-modified DNA probe, which could detect the mature products of both MIR396a and MIR396b. As shown in Fig. 5E, the LNA signals were detected in the SAM and leaf primordia, and appeared to have a higher level in the distal part of primoridia, but no apparent polar distribution along the adaxial–abaxial axis was observed
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleIn addition to histone H4, the expression of several other cell cycle-related marker genes was also analysed by qRT-PCR. Cyclin D3;1 (CycD3;1) is mainly expressed at proliferating cells in the G1 phase and drives the G1/S transition together with cyclin-dependent kinase A (CDKA) (Dewitte et al., 2003; Menges et al., 2006). CYCA2;1 is involved in S/G2 transition, and CYCB1;1 in G2/M transition (Doerner et al., 1996; Shaul et al., 1996 ... the mRNA levels of cell cycle marker genes were reduced even more (5- to 15-fold change) in 35S:miR396a/as2 plants (Fig. S2D) as compared with those in the as2 mutant (Fig. S2A, D
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed Articlefunctional domain of the promoters of MIR396A and MIR396B in the leaves was therefore characterized to better understand the expression pattern of miR396. The ∼3.2 kb 5' upstream regions of MIR396A and MIR396B (counting from the first nucleotide of the mature miR396 sequences) were cloned to represent their respectiveputative promoters. The fragments were fused with the GUS reporter gene to generate the p396a:GUS and p396b:GUS constructs (Fig. 5H), which were then introduced into wild-type plants. By examining the GUS staining signals, it was found that dynamic but similar patterns for both constructs were detected from the cotyledon, shoot apex, and rosette leaves ... In the cotyledon, the GUS signals were very strong (Fig. 5I–J), in contrast to almost no signals of CYCB1;1:GUS
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleTo obtain molecular evidence that miR396-targeted AtGRFs are required for establishment of leaf polarity, the expression pattern of a YABBY family gene, FIL/YAB1, was examined by in situ hybridization. FIL is usually expressed on the leaf abaxial domain of both the wild type (Fig. 3J) and the as2 mutant (Fig. 3K
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleIn addition to histone H4, the expression of several other cell cycle-related marker genes was also analysed by qRT-PCR. Cyclin D3;1 (CycD3;1) is mainly expressed at proliferating cells in the G1 phase and drives the G1/S transition together with cyclin-dependent kinase A (CDKA) (Dewitte et al., 2003; Menges et al., 2006). CYCA2;1 is involved in S/G2 transition, and CYCB1;1 in G2/M transition (Doerner et al., 1996; Shaul et al., 1996). The expression of all the tested marker genes was reduced in 35S:miR396a plants with a 2- to 4-fold change
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleThe transcription levels of AtGRF genes were analysed in 35S:miR396a/as2 transgenic plants by qRT-PCR. Similar to 35S:miR396/Col transgenic plants, the expression levels of AtGRF genes were decreased in 35S:miR396a/as2 (Fig. 1J), suggesting that the leaf polarity defects in 35S:miR396a/as2 plants were due to the decreased expression of AtGRF genes
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleThe expression of cell cycle-related genes was also examined in as2 and 35S:miR396a/as2 plants. Similarly, the number of cells expressing histone H4 was reduced (Supplementary Fig. S2B, C at JXB online), and the mRNA levels of cell cycle marker genes were reduced even more (5- to 15-fold change) in 35S:miR396a/as2 plants (Fig. S2D) as compared with those in the as2 mutant (Fig. S2A, D
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleInterestingly, the transcripts of AtGIF1/AN3 appeared to be highly accumulated in the proximal part of leaf primordia (Fig. 5C), in which cells are rapidly proliferating, consistent with the previous results obtained by promoter–GUS assay
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleThe transcription levels of AtGRF genes were analysed in 35S:miR396a/as2 transgenic plants by qRT-PCR. Similar to 35S:miR396/Col transgenic plants, the expression levels of AtGRF genes were decreased in 35S:miR396a/as2 (Fig. 1J), suggesting that the leaf polarity defects in 35S:miR396a/as2 plants were due to the decreased expression of AtGRF genes
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleqRT-PCR analysis revealed that the transcription levels of the nine AtGRF genes including seven predicated AtGRF gene targets in 35S:miR396a and 35S:miR396b plants were all decreased
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleThe transcription levels of AtGRF genes were analysed in 35S:miR396a/as2 transgenic plants by qRT-PCR. Similar to 35S:miR396/Col transgenic plants, the expression levels of AtGRF genes were decreased in 35S:miR396a ... as2 (Fig. 1J), suggesting that the leaf polarity defects in 35S:miR396a/as2 plants were due to the decreased expression of AtGRF genes
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleThe transcription levels of AtGRF genes were analysed in 35S:miR396a/as2 transgenic plants by qRT-PCR. Similar to 35S:miR396/Col transgenic plants, the expression levels of AtGRF genes were decreased in 35S:miR396a/as2 (Fig. 1J), suggesting that the leaf polarity defects in 35S:miR396a/as2 plants were due to the decreased expression of AtGRF genes
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleqRT-PCR analysis revealed that the transcription levels of the nine AtGRF genes including seven predicated AtGRF gene targets in 35S:miR396a and 35S:miR396b plants were all decreased
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleqRT-PCR analysis revealed that the transcription levels of the nine AtGRF genes including seven predicated AtGRF gene targets in 35S:miR396a and 35S:miR396b plants were all decreased
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleThe transcription levels of AtGRF genes were analysed in 35S:miR396a/as2 transgenic plants by qRT-PCR. Similar to 35S:miR396/Col transgenic plants, the expression levels of AtGRF genes were decreased in 35S:miR396a/as2 (Fig. 1J), suggesting that the leaf polarity defects in 35S:miR396a/as2 plants were due to the decreased expression of AtGRF genes
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleIn addition to histone H4, the expression of several other cell cycle-related marker genes was also analysed by qRT-PCR. Cyclin D3;1 (CycD3;1) is mainly expressed at proliferating cells in the G1 phase and drives the G1/S transition together with cyclin-dependent kinase A (CDKA) (Dewitte et al., 2003; Menges et al., 2006). CYCA2;1 is involved in S/G2 transition, and CYCB1;1 in G2/M transition (Doerner et al., 1996; Shaul et al., 1996). The expression of all the tested marker genes was reduced in 35S:miR396a plants with a 2- to 4-fold change
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleIn addition to histone H4, the expression of several other cell cycle-related marker genes was also analysed by qRT-PCR. Cyclin D3;1 (CycD3;1) is mainly expressed at proliferating cells in the G1 phase and drives the G1/S transition together with cyclin-dependent kinase A (CDKA) (Dewitte et al., 2003; Menges et al., 2006). CYCA2;1 is involved in S/G2 transition, and CYCB1;1 in G2/M transition (Doerner et al., 1996; Shaul et al., 1996). The expression of all the tested marker genes was reduced in 35S:miR396a plants with a 2- to 4-fold change
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleThe transcription levels of AtGRF genes were analysed in 35S:miR396a/as2 transgenic plants by qRT-PCR. Similar to 35S:miR396/Col transgenic plants, the expression levels of AtGRF genes were decreased in 35S:miR396a/as2 (Fig. 1J), suggesting that the leaf polarity defects in 35S:miR396a/as2 plants were due to the decreased expression of AtGRF genes
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleThe transcription levels of AtGRF genes were analysed in 35S:miR396a/as2 transgenic plants by qRT-PCR. Similar to 35S:miR396/Col transgenic plants, the expression levels of AtGRF genes were decreased in 35S:miR396a/as2 (Fig. 1J), suggesting that the leaf polarity defects in 35S:miR396a/as2 plants were due to the decreased expression of AtGRF genes
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleThe expression pattern of miR396 was further investigated using an antisense LNA-modified DNA probe, which could detect the mature products of both MIR396a and MIR396b. As shown in Fig. 5E, the LNA signals were detected in the SAM and leaf primordia, and appeared to have a higher level in the distal part of primoridia, but no apparent polar distribution along the adaxial–abaxial axis was observed
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleTo analyse the temporal–spatial regulation of cell division by miR396–AtGRF modules, the expression patterns of AtGRF2, AtGRF9, and AtGIF1/AN3 were first examined by in situ hybridization. AtGRF2 and AtGRF9 were expressed in the SAM and young leaf primordium
Wang L, Gu X, Xu D, Wang W, Wang H, Zeng M, Chang Z, Huang H, Cui X - miR396-targeted AtGRF transcription factors are required for coordination of cell division and differentiation during leaf development in Arabidopsis
Visit the PubMed ArticleAt about 14 days post-germination ... For LAC8p::GUS very faint staining was detected only in leaf primordia
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleBased on RT-PCR analysis, 15 laccases displayed some level of expression in most tissues ... Fig. 5
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleBased on RT-PCR analysis, 15 laccases displayed some level of expression in most tissues ... Fig. 5
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleGUS-staining in mature leaves ... expression of LAC8p::GUS was very faint, mostly being restricted to tertiary veins
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleBased on RT-PCR analysis, 15 laccases displayed some level of expression in most tissues ... Fig. 5
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleGUS-staining in mature leaves ... staining was observed in mesophyll tissue and supporting trichome epidermal cells ... in the latter cell types, expression of LAC2p::GUS was also noted
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleGUS-staining in mature leaves ... For ... LAC3p::GUS, diffuse staining was observed in mesophyll tissue
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleGUS-staining in mature leaves ... Rosette leaf expression was prominent in vasculature of ... LAC9p::GUS
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleAt about 14 days post-germination ... No expression of ... LAC16p::GUS was observed in aerial organs
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleBased on RT-PCR analysis, 15 laccases displayed some level of expression in most tissues ... Fig. 5
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleBased on RT-PCR analysis, 15 laccases displayed some level of expression in most tissues ... Fig. 5
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleGUS-staining in mature leaves ... Rosette leaf expression was prominent in vasculature of ... LAC4p::GUS
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleAt about 14 days post-germination ... the first set of true leaves ... expression patterns for ... LAC9p::GUS–LAC11p::GUS ... were ... staining ... in trichomes
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleGUS-staining in mature leaves ... Rosette leaf expression was prominent in vasculature of LAC2p::GUS
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleAt about 14 days post-germination ... faint expression levels in leaf vasculature were observed for ... LAC12p::GUS
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleBased on RT-PCR analysis, 15 laccases displayed some level of expression in most tissues ... Fig. 5
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleAt about 14 days post-germination ... the first set of true leaves ... expression patterns for ... LAC15p::GUS ... were ... staining in the vasculature of ... rosette leaves
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleBased on RT-PCR analysis, 15 laccases displayed some level of expression in most tissues ... Fig. 5
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleGUS-staining in mature leaves ... Rosette leaf expression was prominent in vasculature of ... LAC12p::GUS
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleGUS-staining in mature leaves ... For LAC7p::GUS, expression was mainly restricted to hydathodes
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleAt about 14 days post-germination ... the first set of true leaves ... expression patterns for ... LAC4p::GUS ... were ... staining ... in trichomes
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleAt about 14 days post-germination ... the first set of true leaves ... expression patterns for ... LAC4p::GUS ... were ... staining in the vasculature of ... rosette leaves
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleGUS-staining in mature leaves ... LAC3p::GUS ... staining was observed in ... supporting trichome epidermal cells
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleAt about 14 days post-germination ... Expression patterns of ... LAC7p::GUS ... were ... mainly noted in leaf trichomes
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleAt about 14 days post-germination ... the first set of true leaves ... expression patterns for ... LAC9p::GUS–LAC11p::GUS ... were ... staining in the vasculature of ... rosette leaves
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleAt about 14 days post-germination ... the first set of true leaves ... expression patterns for ... LAC14p::GUS ... were ... staining in the vasculature of ... rosette leaves
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleAt about 14 days post-germination ... the first set of true leaves ... expression patterns for ... LAC9p::GUS ... were ... staining ... in trichomes
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleAt about 14 days post-germination ... the first set of true leaves ... expression patterns for LAC2p::GUS ... were ... staining ... in trichomes
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleGUS-staining in mature leaves ... For LAC1p::GUS ... staining was observed in ... supporting trichome epidermal cells
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleAt about 14 days post-germination ... the first set of true leaves ... expression patterns for ... LAC11p::GUS ... were ... staining in the vasculature of ... rosette leaves
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleAt about 14 days post-germination ... the first set of true leaves ... expression patterns for LAC2p::GUS ... were ... staining in the vasculature of ... rosette leaves
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleGUS-staining in mature leaves ... Rosette leaf expression was prominent in vasculature of ... LAC17p::GUS
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleAt about 14 days post-germination ... the first set of true leaves ... expression patterns for ... LAC9p::GUS ... were ... staining in the vasculature of ... rosette leaves
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleGUS-staining in mature leaves ... Rosette leaf expression was prominent in vasculature of ... LAC9p::GUS–LAC12p
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleGUS-staining in mature leaves ... Rosette leaf expression was prominent in vasculature of ... LAC14p::GUS
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleAt about 14 days post-germination ... the first set of true leaves ... expression patterns for ... LAC14p::GUS ... were ... staining ... in trichomes
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleAt about 14 days post-germination ... No expression of LAC6p::GUS ... was observed in aerial organs
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleAt about 14 days post-germination ... the first set of true leaves ... expression patterns for ... LAC17p::GUS ... were ... staining in the vasculature of ... rosette leaves
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleAt about 14 days post-germination ... Expression patterns of LAC1p::GUS ... were ... mainly noted in leaf trichomes
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleGUS-staining in mature leaves ... Rosette leaf expression was prominent in vasculature of ... LAC5p::GUS
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleGUS-staining in mature leaves ... Rosette leaf expression was prominent in vasculature of ... LAC15p::GUS and
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleGUS-staining in mature leaves ... Expression for LAC6p::GUS ... was, however, not evident in rosette leaves
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleAt about 14 days post-germination ... No expression of ... LAC13p::GUS ... was observed in aerial organs
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleAt about 14 days post-germination ... the first set of true leaves ... expression patterns for ... LAC11p::GUS ... were ... staining ... in trichomes
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleAt about 14 days post-germination ... the first set of true leaves ... expression patterns for ... LAC17p::GUS ... were ... staining ... in trichomes
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleAt about 14 days post-germination ... the first set of true leaves ... expression patterns for ... LAC15p::GUS ... were ... staining ... in trichomes
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleBased on RT-PCR analysis, 15 laccases displayed some level of expression in most tissues ... Fig. 5
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleGUS-staining in mature leaves ... For LAC1p::GUS ... diffuse staining was observed in mesophyll tissue
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleGUS-staining in mature leaves ... Rosette leaf expression was prominent in vasculature of ... LAC9p::GUS–LAC12p
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleBased on RT-PCR analysis, 15 laccases displayed some level of expression in most tissues ... Fig. 5
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleGUS-staining in mature leaves ... Expression for ... LAC16p::GUS was, however, not evident in rosette leaves
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleAt about 14 days post-germination ... faint expression levels in leaf vasculature were observed for LAC5p::GUS
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleAt about 14 days post-germination ... Expression patterns of ... LAC3p::GUS ... were ... mainly noted in leaf trichomes
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleGUS-staining in mature leaves ... Expression for ... LAC13p::GUS ... was, however, not evident in rosette leaves
Turlapati PV, Kim KW, Davin LB, Lewis NG - The laccase multigene family in Arabidopsis thaliana: towards addressing the mystery of their gene function(s)
Visit the PubMed ArticleSIM was the most highly expressed cell cycle related gene in gl3-sst nok
Gilding EK, Marks MD - Analysis of purified glabra3-shapeshifter trichomes reveals a role for NOECK in regulating early trichome morphogenic events
Visit the PubMed ArticleAlthough ROP2 was expressed at similar levels in mature wild-type (583 ± 121) and gl3-sst trichomes, RIC4, a target of ROP2, was only detected in gl3-sst
Gilding EK, Marks MD - Analysis of purified glabra3-shapeshifter trichomes reveals a role for NOECK in regulating early trichome morphogenic events
Visit the PubMed ArticleGL1 expression was 3.4-fold higher (P < 0.05) in gl3-sst trichomes. This finding was important because previous analyses showed that GL1 expression is highest in early stage trichomes (Larkin et al., 1993). Compared with gl3-sst sim trichomes, GL1 expression in gl3-sst was 7.8-fold lower (Marks et al., 2009)
Gilding EK, Marks MD - Analysis of purified glabra3-shapeshifter trichomes reveals a role for NOECK in regulating early trichome morphogenic events
Visit the PubMed ArticleAn intriguing difference between gl3-sst and gl3-sst nok trichomes is that ROP2 was expressed five-fold higher in gl3-sst ... Although ROP2 was expressed at similar levels in mature wild-type (583 ± 121) and gl3-sst trichomes
Gilding EK, Marks MD - Analysis of purified glabra3-shapeshifter trichomes reveals a role for NOECK in regulating early trichome morphogenic events
Visit the PubMed ArticleROP11 ... was expressed three-fold higher in gl3-sst nok (2755 ± 463) than in gl3-sst (824 ± 218
Gilding EK, Marks MD - Analysis of purified glabra3-shapeshifter trichomes reveals a role for NOECK in regulating early trichome morphogenic events
Visit the PubMed ArticleA previous study indicated that TRY expression was reduced or absent in gl3-sst trichomes (Esch et al., 2004). In this study, the comparison between gl3-sst and wild-type showed that TRY expression was reduced by a factor of 2.52 (P < 0.05
Gilding EK, Marks MD - Analysis of purified glabra3-shapeshifter trichomes reveals a role for NOECK in regulating early trichome morphogenic events
Visit the PubMed ArticleThere were some significant differences in the gl3-sst sim and gl3-sst nok profiles concerning the expression of genes involved in the cell cycle. For example, CDKB2s 1 and 2, which promote cell division, were more highly expressed in gl3-sst sim [(295 ± 128 and 322 ± 147 versus 108 ± 27 and not detected
Gilding EK, Marks MD - Analysis of purified glabra3-shapeshifter trichomes reveals a role for NOECK in regulating early trichome morphogenic events
Visit the PubMed ArticleThere were some significant differences in the gl3-sst sim and gl3-sst nok profiles concerning the expression of genes involved in the cell cycle. For example, CDKB2s 1 and 2, which promote cell division, were more highly expressed in gl3-sst sim [(295 ± 128 and 322 ± 147 versus 108 ± 27 and not detected
Gilding EK, Marks MD - Analysis of purified glabra3-shapeshifter trichomes reveals a role for NOECK in regulating early trichome morphogenic events
Visit the PubMed ArticleThe transcriptome of gl3-sst nok ... MYB30 and associated lipid metabolism related genes also were highly expressed
Gilding EK, Marks MD - Analysis of purified glabra3-shapeshifter trichomes reveals a role for NOECK in regulating early trichome morphogenic events
Visit the PubMed ArticleGL1 expression was higher in ... gl3-sst nok ... than in gl3-sst trichomes
Gilding EK, Marks MD - Analysis of purified glabra3-shapeshifter trichomes reveals a role for NOECK in regulating early trichome morphogenic events
Visit the PubMed Articlethe comparison between gl3-sst and wild-type showed that ... CPC which functions redundantly with TRY, was expressed 6.64-fold lower
Gilding EK, Marks MD - Analysis of purified glabra3-shapeshifter trichomes reveals a role for NOECK in regulating early trichome morphogenic events
Visit the PubMed ArticleRNA was extracted from trichomes isolated from three biological replicates of gl3-sst plants. The RNA was used to generate probes for hybridization to the Affymetrix ATH1 GeneChip® as previously described [(Marks et al., 2008, 2009); see Table S1 for the normalized means and standard deviation for all genes declared detected (i.e. Affymetrix MAS5 P < 0.04) with all values normalized to 1000 per Chip]. The replicates were grown under different conditions and the samples were processed at two locations ... To identify predominant cellular activities in gl3-sst trichomes, a Gene Set Enrichment Analysis (GSEA) was performed on highly expressed genes using GeneTrail (http://genetrail.bioinf.uni-sb.de/index.php). The complete output of the analysis of 3245 genes is shown in Table S2. The largest enriched set of genes (427 of 3245) contained those involved in stress responses (GO:0006950) and 35 of these genes were found among the 100 most highly expressed. One of the more prevalent subsets of stress genes corresponded to those involved in dealing with osmotic stress (149; GO:0006970). Other sets of more highly expressed genes were involved in translation (229; GO:0006412), cell walls (195; GO:0005618), vacuoles (262; GO:0005773), plasma membrane (719; GO:0005886), and oxidative phosphorylation (32; GO:0006119
Gilding EK, Marks MD - Analysis of purified glabra3-shapeshifter trichomes reveals a role for NOECK in regulating early trichome morphogenic events
Visit the PubMed ArticleThe transcriptome of gl3-sst nok resembled that of gl3-sst sim (gl3-sst sim data available from ArrayExpress and Marks et al., 2009). In both, the genes encoding proteins involved in translation or ribosome structure were the most highly expressed (represented by approximately 50% of the 150 most highly expressed genes in both datasets
Gilding EK, Marks MD - Analysis of purified glabra3-shapeshifter trichomes reveals a role for NOECK in regulating early trichome morphogenic events
Visit the PubMed ArticleA search was conducted to identify transcription factors more highly expressed in gl3-sst trichomes than in wild-type mature trichomes. This search led to the detailed analysis of AT3G01140 (MYB106), which was expressed six-fold higher
Gilding EK, Marks MD - Analysis of purified glabra3-shapeshifter trichomes reveals a role for NOECK in regulating early trichome morphogenic events
Visit the PubMed ArticleThere were some significant differences in the gl3-sst sim and gl3-sst nok profiles concerning the expression of genes involved in the cell cycle ... CDC6 expression, which is required for the initiation of DNA replication and endoreduplication, was expressed at a higher level in gl3-sst nok trichomes
Gilding EK, Marks MD - Analysis of purified glabra3-shapeshifter trichomes reveals a role for NOECK in regulating early trichome morphogenic events
Visit the PubMed ArticleThere were some significant differences in the gl3-sst sim and gl3-sst nok profiles concerning the expression of genes involved in the cell cycle ... CDKA;1, which is required for both endoreduplication and cell division, was expressed at higher levels in gl3-sst nok
Gilding EK, Marks MD - Analysis of purified glabra3-shapeshifter trichomes reveals a role for NOECK in regulating early trichome morphogenic events
Visit the PubMed ArticleTo identify other differences between gl3-sst and wild-type, several types of analyses were performed. First, genes declared detected in gl3-sst trichomes but not in wild-type trichomes were identified. For those genes detected in both sets, the ratios between the expression values were determined and subjected to a t-test (see Table S3 online for complete results). A list containing genes only detected in gl3-sst (and not in wild-type trichomes) along with those expressed at least 2.5-fold higher in gl3-sst than wild-type was used in an overrepresentation analysis (ORA) using GeneTool. It was found that genes involved in lipid metabolic processes (GO:0006629) were specifically enriched in gl3-sst (P-value (fdr) < 8.56 × 10−6) (Table S4). These genes included those involved in fatty acid and wax biosynthesis. Genes involved in trichome differentiation (GO:0010026) also were over represented [P-value (fdr) < 0.011
Gilding EK, Marks MD - Analysis of purified glabra3-shapeshifter trichomes reveals a role for NOECK in regulating early trichome morphogenic events
Visit the PubMed ArticleAn interesting contrast and potentially inconsistent finding was the elevated expression in gl3-sst nok (1584 ± 358) versus gl3-sst sim (206 ± 62) of LOG7, which encodes cytokinin riboside 5′-monophosphate phosphoribohydrolase that promotes cell division
Gilding EK, Marks MD - Analysis of purified glabra3-shapeshifter trichomes reveals a role for NOECK in regulating early trichome morphogenic events
Visit the PubMed ArticleKNAT2 ... transcript levels were ... elevated in ae7 ... mutant leaves
Yuan Z, Luo D, Li G, Yao X, Wang H, Zeng M, Huang H, Cui X - Characterization of the AE7 gene in Arabidopsis suggests that normal cell proliferation is essential for leaf polarity establishment
Visit the PubMed ArticleRAD51 ... was analyzed by RT-PCR and found to be upregulated in ae7
Yuan Z, Luo D, Li G, Yao X, Wang H, Zeng M, Huang H, Cui X - Characterization of the AE7 gene in Arabidopsis suggests that normal cell proliferation is essential for leaf polarity establishment
Visit the PubMed Articlewe analyzed expression levels of several cell-cycle marker genes by qRT-PCR ... in the ae7 mutant ... CYCD3;1 ... unaltered
Yuan Z, Luo D, Li G, Yao X, Wang H, Zeng M, Huang H, Cui X - Characterization of the AE7 gene in Arabidopsis suggests that normal cell proliferation is essential for leaf polarity establishment
Visit the PubMed Articlewe analyzed expression levels of several cell-cycle marker genes by qRT-PCR. The transcript levels of ... in the ae7 mutant ... H4 ... unaltered
Yuan Z, Luo D, Li G, Yao X, Wang H, Zeng M, Huang H, Cui X - Characterization of the AE7 gene in Arabidopsis suggests that normal cell proliferation is essential for leaf polarity establishment
Visit the PubMed Articlewe analyzed expression levels of several cell-cycle marker genes by qRT-PCR. The transcript levels of the G2/Mphase- specific genes, CYCB1;1 ... were increased in the ae7 mutant
Yuan Z, Luo D, Li G, Yao X, Wang H, Zeng M, Huang H, Cui X - Characterization of the AE7 gene in Arabidopsis suggests that normal cell proliferation is essential for leaf polarity establishment
Visit the PubMed ArticleKNAT2 ... transcript levels were ... elevated in ... ae7 as2-101 double mutant leaves
Yuan Z, Luo D, Li G, Yao X, Wang H, Zeng M, Huang H, Cui X - Characterization of the AE7 gene in Arabidopsis suggests that normal cell proliferation is essential for leaf polarity establishment
Visit the PubMed ArticleWe also analyzed H4 hybridization signals in the ae7 mutant, but no significant changes in the H4 pattern were observed between wild-type Ler and ae7
Yuan Z, Luo D, Li G, Yao X, Wang H, Zeng M, Huang H, Cui X - Characterization of the AE7 gene in Arabidopsis suggests that normal cell proliferation is essential for leaf polarity establishment
Visit the PubMed Articlewe examined expression of BP, one of the class I KNOX genes in leaves by introducing a pBP:GUS fusion into the ae7 mutant. In wild- type leaves, the GUS reporter gene is usually repressed (Figure 2k), whereas in the ae7 mutant leaves, GUS signals were indeed ectopically distributed in the leaf vasculature and sinuses of serrations
Yuan Z, Luo D, Li G, Yao X, Wang H, Zeng M, Huang H, Cui X - Characterization of the AE7 gene in Arabidopsis suggests that normal cell proliferation is essential for leaf polarity establishment
Visit the PubMed ArticleIn seedlings, AE7 signals accumulated throughout the early-stage leaf primordia (Figure 5e) with a spotted pattern
Yuan Z, Luo D, Li G, Yao X, Wang H, Zeng M, Huang H, Cui X - Characterization of the AE7 gene in Arabidopsis suggests that normal cell proliferation is essential for leaf polarity establishment
Visit the PubMed ArticleLongitudinal sections showed that in addition to the leaf primordia, AE7 signals accumulated in the SAM
Yuan Z, Luo D, Li G, Yao X, Wang H, Zeng M, Huang H, Cui X - Characterization of the AE7 gene in Arabidopsis suggests that normal cell proliferation is essential for leaf polarity establishment
Visit the PubMed ArticleBP ... transcript levels were ... elevated in ... ae7 as2-101 double mutant leaves
Yuan Z, Luo D, Li G, Yao X, Wang H, Zeng M, Huang H, Cui X - Characterization of the AE7 gene in Arabidopsis suggests that normal cell proliferation is essential for leaf polarity establishment
Visit the PubMed ArticleAt1g68310 ... RT-PCR analyses indicated that this gene was expressed in all plant tissues examined, including the root, stem, rosette leaf, cauline leaf, inflorescence and silique
Yuan Z, Luo D, Li G, Yao X, Wang H, Zeng M, Huang H, Cui X - Characterization of the AE7 gene in Arabidopsis suggests that normal cell proliferation is essential for leaf polarity establishment
Visit the PubMed ArticleKNAT6 ... transcript levels were ... elevated in ae7 ... mutant leaves
Yuan Z, Luo D, Li G, Yao X, Wang H, Zeng M, Huang H, Cui X - Characterization of the AE7 gene in Arabidopsis suggests that normal cell proliferation is essential for leaf polarity establishment
Visit the PubMed Articlewe analyzed expression levels of several cell-cycle marker genes by qRT-PCR. The transcript levels of ... in the ae7 mutant ... CYCA2;1 ... unaltered
Yuan Z, Luo D, Li G, Yao X, Wang H, Zeng M, Huang H, Cui X - Characterization of the AE7 gene in Arabidopsis suggests that normal cell proliferation is essential for leaf polarity establishment
Visit the PubMed ArticleIn situ hybridization, using a gene-specific sequence as a probe, showed that AE7 was expressed throughout the globular-stage (Figure 5a), heart-stage (Figure 5b) and torpedo- stage (Figure 5c) embryos
Yuan Z, Luo D, Li G, Yao X, Wang H, Zeng M, Huang H, Cui X - Characterization of the AE7 gene in Arabidopsis suggests that normal cell proliferation is essential for leaf polarity establishment
Visit the PubMed ArticleIn the late-stage leaf primordia, both AE7 and H4 signals were detected, except in the central part of the abaxial domain, where the midvein cells were enlarging
Yuan Z, Luo D, Li G, Yao X, Wang H, Zeng M, Huang H, Cui X - Characterization of the AE7 gene in Arabidopsis suggests that normal cell proliferation is essential for leaf polarity establishment
Visit the PubMed ArticleAt the bent-cotyledon stage, the AE7 signal became weaker
Yuan Z, Luo D, Li G, Yao X, Wang H, Zeng M, Huang H, Cui X - Characterization of the AE7 gene in Arabidopsis suggests that normal cell proliferation is essential for leaf polarity establishment
Visit the PubMed ArticleWEE1 ... was analyzed by RT-PCR and found to be upregulated in ae7
Yuan Z, Luo D, Li G, Yao X, Wang H, Zeng M, Huang H, Cui X - Characterization of the AE7 gene in Arabidopsis suggests that normal cell proliferation is essential for leaf polarity establishment
Visit the PubMed ArticleKNAT6 ... transcript levels were ... elevated in ... ae7 as2-101 double mutant leaves
Yuan Z, Luo D, Li G, Yao X, Wang H, Zeng M, Huang H, Cui X - Characterization of the AE7 gene in Arabidopsis suggests that normal cell proliferation is essential for leaf polarity establishment
Visit the PubMed Articlewe analyzed expression levels of several cell-cycle marker genes by qRT-PCR. The transcript levels of ... CDKB1;1 ... were increased in the ae7 mutant
Yuan Z, Luo D, Li G, Yao X, Wang H, Zeng M, Huang H, Cui X - Characterization of the AE7 gene in Arabidopsis suggests that normal cell proliferation is essential for leaf polarity establishment
Visit the PubMed ArticleBP ... transcript levels were ... elevated in ae7 ... mutant leaves
Yuan Z, Luo D, Li G, Yao X, Wang H, Zeng M, Huang H, Cui X - Characterization of the AE7 gene in Arabidopsis suggests that normal cell proliferation is essential for leaf polarity establishment
Visit the PubMed ArticleUsing an ATML1 probe as a marker for L1 identity (Lu et al., 1996), we found that, as in the wild-type, only the outermost cell layer of lom1 lom2 lom3 mutants had L1 identity
Schulze S, Schäfer BN, Parizotto EA, Voinnet O, Theres K - LOST MERISTEMS genes regulate cell differentiation of central zone descendants in Arabidopsis shoot meristems
Visit the PubMed ArticleHybridization of consecutive lom1 lom2 lom3 shoot tip sections alternatively with WUS (Figure 5c) and CLV3 (Figure 5d) probes revealed that the CLV3 expression domain completely encompassed the WUS mRNA domain and exceeded it in all directions. These findings demonstrate that the relative positions of WUS- and CLV3-expressing cell groups within lom1 lom2 lom3 SAMs were altered, suggesting disruption of their functional interplay
Schulze S, Schäfer BN, Parizotto EA, Voinnet O, Theres K - LOST MERISTEMS genes regulate cell differentiation of central zone descendants in Arabidopsis shoot meristems
Visit the PubMed ArticleLOM2 ... transcripts accumulated in the SAM
Schulze S, Schäfer BN, Parizotto EA, Voinnet O, Theres K - LOST MERISTEMS genes regulate cell differentiation of central zone descendants in Arabidopsis shoot meristems
Visit the PubMed ArticleAthb23 encodes a homeodomain-leucine zipper (HD-ZIP) protein that is specifically expressed in the rib zone (Figure 5g) and the adaxial domain of leaf primordia (Kim et al., 2007). Examination of ATHB23:GUS expression in lom1 lom2 mutants revealed GUS expression in the small cells between the WUS expression domain and the large cells of the rib zone (Figure 5h). This finding indicates that these cells acquire rib zone identity. However, unlike in the wild-type, they do not differentiate
Schulze S, Schäfer BN, Parizotto EA, Voinnet O, Theres K - LOST MERISTEMS genes regulate cell differentiation of central zone descendants in Arabidopsis shoot meristems
Visit the PubMed ArticleLOM3 mRNA was detected only in the boundary region between leaf primordia and the vegetative shoot apical meristem
Schulze S, Schäfer BN, Parizotto EA, Voinnet O, Theres K - LOST MERISTEMS genes regulate cell differentiation of central zone descendants in Arabidopsis shoot meristems
Visit the PubMed ArticleIn wild-type apices, CLV3 transcripts accumulate in a triangular cell group localized above the WUS expression domain at the distal tip of the SAM (Figure 5b). In the lom triple mutant, this expression pattern was expanded to include the amplified tunica cell layers, but excluded the outermost cell layers of the SAM (Figure 5d). A basipetal shift in the CLV3 expression pattern was observed in the youngest shoot apices analyzed at 21 DAG (Figure S7j–l). Hybridization of consecutive lom1 lom2 lom3 shoot tip sections alternatively with WUS (Figure 5c) and CLV3 (Figure 5d) probes revealed that the CLV3 expression domain completely encompassed the WUS mRNA domain and exceeded it in all directions. These findings demonstrate that the relative positions of WUS- and CLV3-expressing cell groups within lom1 lom2 lom3 SAMs were altered, suggesting disruption of their functional interplay
Schulze S, Schäfer BN, Parizotto EA, Voinnet O, Theres K - LOST MERISTEMS genes regulate cell differentiation of central zone descendants in Arabidopsis shoot meristems
Visit the PubMed ArticleLOM1 transcripts were detected in the vascular strands of young leaf primordia
Schulze S, Schäfer BN, Parizotto EA, Voinnet O, Theres K - LOST MERISTEMS genes regulate cell differentiation of central zone descendants in Arabidopsis shoot meristems
Visit the PubMed ArticleLOM2 ... transcripts were detected in the vascular strands of young leaf primordia
Schulze S, Schäfer BN, Parizotto EA, Voinnet O, Theres K - LOST MERISTEMS genes regulate cell differentiation of central zone descendants in Arabidopsis shoot meristems
Visit the PubMed ArticleTo determine whether cessation of leaf formation is correlated with an alteration in the pattern of cell divisions in the SAM, we monitored accumulation of S phase-associated histone H4 mRNA in shoot apices. As the wild-type, lom triple mutants showed a higher density of histone H4-expressing cells in the peripheral zone than in the central zone of the meristem ... reflecting the increase in cell divisions in the peripheral zone that is required for the formation of leaf primordia
Schulze S, Schäfer BN, Parizotto EA, Voinnet O, Theres K - LOST MERISTEMS genes regulate cell differentiation of central zone descendants in Arabidopsis shoot meristems
Visit the PubMed Articlethe expression domain of the LAS gene, which spanned only the five or six top-most cell layers in wild-type shoot apices (Figure 4c), extended to at least nine or 10 cell layers in lom1 lom2 mutants
Schulze S, Schäfer BN, Parizotto EA, Voinnet O, Theres K - LOST MERISTEMS genes regulate cell differentiation of central zone descendants in Arabidopsis shoot meristems
Visit the PubMed ArticleLOM2 transcripts were found to accumulate in a pattern very similar to that of LOM1; however, the signals obtained with LOM2 probes were consistently weaker than those obtained with LOM1 probes
Schulze S, Schäfer BN, Parizotto EA, Voinnet O, Theres K - LOST MERISTEMS genes regulate cell differentiation of central zone descendants in Arabidopsis shoot meristems
Visit the PubMed ArticleIn lom triple mutants, STM transcripts were initially detected in a pattern similar to that of wild-type plants (Figure 4e). With the increase in meristem width, the STM expression domain expanded laterally from approximately 100 μm at 28 DAG to approximately 150 μm at 49 DAG (Figure S7). STM mRNA was also detected in the additional cell layers that developed in lom1 lom2 lom3 shoot apices. This indicated that, even several weeks after cessation of leaf primordium formation, most cells in the lom1 lom2 lom3 shoot meristem had not lost their meristematic identity. However, from 42 DAG onwards, STM mRNA accumulation was strongly reduced in the topmost extra cell layers of the meristem
Schulze S, Schäfer BN, Parizotto EA, Voinnet O, Theres K - LOST MERISTEMS genes regulate cell differentiation of central zone descendants in Arabidopsis shoot meristems
Visit the PubMed ArticleLOM1 transcripts accumulated in the SAM from the L2 layer downwards to the rib meristem ... with higher levels in the peripheral zone than in the central zone of the meristem
Schulze S, Schäfer BN, Parizotto EA, Voinnet O, Theres K - LOST MERISTEMS genes regulate cell differentiation of central zone descendants in Arabidopsis shoot meristems
Visit the PubMed ArticleWe used ANT transcript accumulation as a marker (Elliott et al., 1996; Long and Barton, 2000) to assess whether leaf founder cells are established at the flanks of lom1 lom2 lom3 meristems (Figure 4k,l). In the wild-type, ANT mRNA was detected in discrete cell groups of the peripheral zone, the incipient leaf primordia and the vascular strands of developing leaf primordia (Figure 4k). The expression in leaf primordia was not altered in lom triple mutants. However, ANT expression in the meristem expanded into an inverted cup-shaped domain comprising the central zone and the peripheral zone of the SAM and extending towards the rib zone (Figure 4l). This pattern of ANT transcript accumulation indicated over-accumulation of leaf founder cells that do not participate in the formation of leaf primordia
Schulze S, Schäfer BN, Parizotto EA, Voinnet O, Theres K - LOST MERISTEMS genes regulate cell differentiation of central zone descendants in Arabidopsis shoot meristems
Visit the PubMed ArticleTransverse sections revealed more precisely that LOM1 mRNA accumulates in and around the vascular elements along the boundary between the adaxial and the abaxial side of leaf primordia
Schulze S, Schäfer BN, Parizotto EA, Voinnet O, Theres K - LOST MERISTEMS genes regulate cell differentiation of central zone descendants in Arabidopsis shoot meristems
Visit the PubMed ArticleWUS expression in shoot apices of lom triple mutants was monitored at 21, 28, 35, 42 and 49 DAG under short photoperiods (Figure S7e–i). WUS mRNA accumulated in the center of the SAM (Figures 5c and S7e–i) in the triple mutant. However, in comparison to the pattern of expression of WUS mRNA in the wild-type (Figure 5a), the expression domain was often enlarged and had an irregular shape, starting at 21 DAG. The number of cell layers separating the WUS expression domain from the L1 layer increased from six layers at 21 DAG to 12 layers at 49 DAG. In the wild-type, the WUS expression domain is positioned just above the large vacuolated cells of the rib zone (Figure 5a). However, in the lom1 lom2 and lom triple mutants, several layers of small cytoplasmic cells separated the cluster of WUS-expressing cells from the rib zone
Schulze S, Schäfer BN, Parizotto EA, Voinnet O, Theres K - LOST MERISTEMS genes regulate cell differentiation of central zone descendants in Arabidopsis shoot meristems
Visit the PubMed ArticleThe steady-state levels of LHCII proteins were examined by immunoblot analyses performed with antibodies raised against ... minor monomeric CP26 protein ... in gdc1-3 ... CP26 accumulated at the same level between the mutant and the wild type
Cui YL, Jia QS, Yin QQ, Lin GN, Kong MM, Yang ZN - The GDC1 gene encodes a novel ankyrin domain-containing protein that is essential for grana formation in Arabidopsis
Visit the PubMed ArticleIn the gdc1-3 mutant, the D1 ... core subunits of PSII accumulated to low levels
Cui YL, Jia QS, Yin QQ, Lin GN, Kong MM, Yang ZN - The GDC1 gene encodes a novel ankyrin domain-containing protein that is essential for grana formation in Arabidopsis
Visit the PubMed ArticleIn the gdc1-3 mutant ... the PsaA subunit of PSI ... accumulated to similar levels as the wild type
Cui YL, Jia QS, Yin QQ, Lin GN, Kong MM, Yang ZN - The GDC1 gene encodes a novel ankyrin domain-containing protein that is essential for grana formation in Arabidopsis
Visit the PubMed ArticleAT1G50900 expression was examined in the allelic mutant plants using reverse transcription (RT)-PCR and real-time RT-PCR analysis with gene-specific primers. Gene expression levels in ... gdc1-2 ... were ... 3.58% of the wild type
Cui YL, Jia QS, Yin QQ, Lin GN, Kong MM, Yang ZN - The GDC1 gene encodes a novel ankyrin domain-containing protein that is essential for grana formation in Arabidopsis
Visit the PubMed ArticleGDC1 expression was not detected in gdc1-3
Cui YL, Jia QS, Yin QQ, Lin GN, Kong MM, Yang ZN - The GDC1 gene encodes a novel ankyrin domain-containing protein that is essential for grana formation in Arabidopsis
Visit the PubMed ArticleNULL
Cui YL, Jia QS, Yin QQ, Lin GN, Kong MM, Yang ZN - The GDC1 gene encodes a novel ankyrin domain-containing protein that is essential for grana formation in Arabidopsis
Visit the PubMed ArticleGDC1 expression patterns in Arabidopsis were also investigated using a GUS reporter gene fused to its promoter ... In 15-d-old seedlings, GUS activity was detected in the basal rosette leaves
Cui YL, Jia QS, Yin QQ, Lin GN, Kong MM, Yang ZN - The GDC1 gene encodes a novel ankyrin domain-containing protein that is essential for grana formation in Arabidopsis
Visit the PubMed ArticleIn the gdc1-3 mutant ... the cytochrome f subunit of cytochrome b6f ... accumulated to similar levels as the wild type
Cui YL, Jia QS, Yin QQ, Lin GN, Kong MM, Yang ZN - The GDC1 gene encodes a novel ankyrin domain-containing protein that is essential for grana formation in Arabidopsis
Visit the PubMed ArticleThe steady-state levels of LHCII proteins were examined by immunoblot analyses performed with antibodies raised against specific subunits ... The results showed that Lhcb1 levels were substantially reduced ... in gdc1-3
Cui YL, Jia QS, Yin QQ, Lin GN, Kong MM, Yang ZN - The GDC1 gene encodes a novel ankyrin domain-containing protein that is essential for grana formation in Arabidopsis
Visit the PubMed ArticleAT1G50900 expression was examined in the allelic mutant plants using reverse transcription (RT)-PCR and real-time RT-PCR analysis with gene-specific primers. Gene expression levels in gdc1-1 ... were 5.07% ... of the wild type
Cui YL, Jia QS, Yin QQ, Lin GN, Kong MM, Yang ZN - The GDC1 gene encodes a novel ankyrin domain-containing protein that is essential for grana formation in Arabidopsis
Visit the PubMed ArticleIn the gdc1-3 mutant ... D2 core subunits of PSII accumulated to low levels
Cui YL, Jia QS, Yin QQ, Lin GN, Kong MM, Yang ZN - The GDC1 gene encodes a novel ankyrin domain-containing protein that is essential for grana formation in Arabidopsis
Visit the PubMed ArticleGDC1 was highly expressed in ... seedlings
Cui YL, Jia QS, Yin QQ, Lin GN, Kong MM, Yang ZN - The GDC1 gene encodes a novel ankyrin domain-containing protein that is essential for grana formation in Arabidopsis
Visit the PubMed ArticleTargetP program analysis showed that GDC1 is a chloroplast-targeted protein (http://www.cbs.dtu.dk/services/TargetP/; Emanuelsson et al., 2000). The subcellular localization of the protein was confirmed by fusing the full-length coding sequence of GDC1 with the GFP gene and introduced into wild-type plants under the control of the cauliflower mosaic virus 35S promoter. Stable transgenic plants were obtained, and GFP fluorescence of transgenic plants was observed with confocal laser microscopy. GFP fluorescence was colocalized with chlorophyll autofluorescence. This confirmed that GDC1 is a chloroplast-localized protein
Cui YL, Jia QS, Yin QQ, Lin GN, Kong MM, Yang ZN - The GDC1 gene encodes a novel ankyrin domain-containing protein that is essential for grana formation in Arabidopsis
Visit the PubMed ArticleThe steady-state levels of LHCII proteins were examined by immunoblot analyses performed with antibodies raised against specific subunits ... Lhcb2 was slightly reduced in gdc1-3
Cui YL, Jia QS, Yin QQ, Lin GN, Kong MM, Yang ZN - The GDC1 gene encodes a novel ankyrin domain-containing protein that is essential for grana formation in Arabidopsis
Visit the PubMed ArticleThe steady-state levels of LHCII proteins were examined by immunoblot analyses performed with antibodies raised against specific subunits of LHCIIb ... in gdc1-3. Lhcb3 ... accumulated at the same level between the mutant and the wild type
Cui YL, Jia QS, Yin QQ, Lin GN, Kong MM, Yang ZN - The GDC1 gene encodes a novel ankyrin domain-containing protein that is essential for grana formation in Arabidopsis
Visit the PubMed ArticleIn the gdc1-3 mutant ... the β-subunit of ATP synthase accumulated to similar levels as the wild type
Cui YL, Jia QS, Yin QQ, Lin GN, Kong MM, Yang ZN - The GDC1 gene encodes a novel ankyrin domain-containing protein that is essential for grana formation in Arabidopsis
Visit the PubMed ArticleGDC1 was highly expressed in leaves
Cui YL, Jia QS, Yin QQ, Lin GN, Kong MM, Yang ZN - The GDC1 gene encodes a novel ankyrin domain-containing protein that is essential for grana formation in Arabidopsis
Visit the PubMed ArticleThe effects of grana deficiency on protein complexes embedded in thylakoid membranes were examined. The chlorophyll-protein complexes were solubilized from thylakoid membranes using dodecyl-β-D-maltopyranoside and separated by blue native (BN)-PAGE (Schägger et al., 1994). After the first-dimensional separation in the presence of Coomassie Brilliant Blue G-250, the major bands representing PSII supercomplexes, monomeric PSI and dimeric PSII, monomeric PSII, dimeric cytochrome b6f, trimeric LHCII, and monomeric LHCII were resolved in the wild type (Fig. 4A). Most of the bands were also detected in the gdc1-3 mutant. However, the trimeric LHCII was negligible, and band I of the wild type disappeared in the gdc1-3 mutant
Cui YL, Jia QS, Yin QQ, Lin GN, Kong MM, Yang ZN - The GDC1 gene encodes a novel ankyrin domain-containing protein that is essential for grana formation in Arabidopsis
Visit the PubMed ArticleIn addition, we examined the effect of light on GDC1 expression (Fig. 7H). Real-time RT-PCR analysis was carried out using total RNA isolated from leaves harvested at 0, 1, 3, 6, 12, or 24 h after the transfer of 7-d-old dark-grown wild-type plants to light conditions. GDC1 was weakly expressed in the dark (0 h), but after transfer of etiolated plants to light conditions, GDC1 mRNA accumulated in a time-dependent manner
Cui YL, Jia QS, Yin QQ, Lin GN, Kong MM, Yang ZN - The GDC1 gene encodes a novel ankyrin domain-containing protein that is essential for grana formation in Arabidopsis
Visit the PubMed ArticleTo further determine the localization of GDC1 within the chloroplast, we investigated the GDC1-GFP fusion protein in 35S::GDC1-GFP transgenic plants with GFP antibody. A positive signal was detected in the total proteins and thylakoid membrane proteins from 35S::GDC1-GFP transgenic plants
Cui YL, Jia QS, Yin QQ, Lin GN, Kong MM, Yang ZN - The GDC1 gene encodes a novel ankyrin domain-containing protein that is essential for grana formation in Arabidopsis
Visit the PubMed ArticleeIF6 ... detected in the cytoplasm
Guo J, Wang S, Valerius O, Hall H, Zeng Q, Li JF, Weston DJ, Ellis BE, Chen JG - Involvement of Arabidopsis RACK1 in protein translation and its regulation by abscisic acid
Visit the PubMed ArticleUsing quantitative RT-PCR, we conducted a detailed analysis of the expression of RACK1 gene family members in response to ABA treatment. The level of transcripts for all three RACK1 genes was down-regulated as early as 1 h after ABA treatment and remained suppressed thereafter
Guo J, Wang S, Valerius O, Hall H, Zeng Q, Li JF, Weston DJ, Ellis BE, Chen JG - Involvement of Arabidopsis RACK1 in protein translation and its regulation by abscisic acid
Visit the PubMed ArticleRACK1 ... detected in the cytoplasm
Guo J, Wang S, Valerius O, Hall H, Zeng Q, Li JF, Weston DJ, Ellis BE, Chen JG - Involvement of Arabidopsis RACK1 in protein translation and its regulation by abscisic acid
Visit the PubMed ArticleeIF6 ... detected in the ... nucleus
Guo J, Wang S, Valerius O, Hall H, Zeng Q, Li JF, Weston DJ, Ellis BE, Chen JG - Involvement of Arabidopsis RACK1 in protein translation and its regulation by abscisic acid
Visit the PubMed ArticleRACK1 ... detected in the ... nucleus
Guo J, Wang S, Valerius O, Hall H, Zeng Q, Li JF, Weston DJ, Ellis BE, Chen JG - Involvement of Arabidopsis RACK1 in protein translation and its regulation by abscisic acid
Visit the PubMed ArticleTo gain further insights into the biochemical/molecular function of RACK1 in Arabidopsis, we performed global coexpression data analysis (PRIME; http://prime.psc.riken.jp/) to identify genes that are coexpressed with all three RACK1 genes. Surprisingly, we found that more than 80% (128 out of 154) of the genes that are coexpressed with RACK1 encode ribosomal proteins (Supplemental Fig. S3; Supplemental Table S3), implying a potential relationship between RACK1 function and the ribosome complex
Guo J, Wang S, Valerius O, Hall H, Zeng Q, Li JF, Weston DJ, Ellis BE, Chen JG - Involvement of Arabidopsis RACK1 in protein translation and its regulation by abscisic acid
Visit the PubMed ArticleeIF6 ... detected in the ... nucleus
Guo J, Wang S, Valerius O, Hall H, Zeng Q, Li JF, Weston DJ, Ellis BE, Chen JG - Involvement of Arabidopsis RACK1 in protein translation and its regulation by abscisic acid
Visit the PubMed ArticleRACK1 ... detected in the ... nucleus
Guo J, Wang S, Valerius O, Hall H, Zeng Q, Li JF, Weston DJ, Ellis BE, Chen JG - Involvement of Arabidopsis RACK1 in protein translation and its regulation by abscisic acid
Visit the PubMed ArticleUsing quantitative RT-PCR, we conducted a detailed analysis of the expression of RACK1 gene family members in response to ABA treatment. The level of transcripts for all three RACK1 genes was down-regulated as early as 1 h after ABA treatment and remained suppressed thereafter
Guo J, Wang S, Valerius O, Hall H, Zeng Q, Li JF, Weston DJ, Ellis BE, Chen JG - Involvement of Arabidopsis RACK1 in protein translation and its regulation by abscisic acid
Visit the PubMed ArticleThe expression of the eIF6B gene was too low to be detected in seedlings used for quantitative RT-PCR
Guo J, Wang S, Valerius O, Hall H, Zeng Q, Li JF, Weston DJ, Ellis BE, Chen JG - Involvement of Arabidopsis RACK1 in protein translation and its regulation by abscisic acid
Visit the PubMed ArticleTo gain further insights into the biochemical/molecular function of RACK1 in Arabidopsis, we performed global coexpression data analysis (PRIME; http://prime.psc.riken.jp/) to identify genes that are coexpressed with all three RACK1 genes. Surprisingly, we found that more than 80% (128 out of 154) of the genes that are coexpressed with RACK1 encode ribosomal proteins (Supplemental Fig. S3; Supplemental Table S3), implying a potential relationship between RACK1 function and the ribosome complex
Guo J, Wang S, Valerius O, Hall H, Zeng Q, Li JF, Weston DJ, Ellis BE, Chen JG - Involvement of Arabidopsis RACK1 in protein translation and its regulation by abscisic acid
Visit the PubMed ArticleeIF6 ... detected in the cytoplasm
Guo J, Wang S, Valerius O, Hall H, Zeng Q, Li JF, Weston DJ, Ellis BE, Chen JG - Involvement of Arabidopsis RACK1 in protein translation and its regulation by abscisic acid
Visit the PubMed ArticleRACK1 ... detected in the cytoplasm
Guo J, Wang S, Valerius O, Hall H, Zeng Q, Li JF, Weston DJ, Ellis BE, Chen JG - Involvement of Arabidopsis RACK1 in protein translation and its regulation by abscisic acid
Visit the PubMed ArticleeIF6B is only expressed in flower buds
Guo J, Wang S, Valerius O, Hall H, Zeng Q, Li JF, Weston DJ, Ellis BE, Chen JG - Involvement of Arabidopsis RACK1 in protein translation and its regulation by abscisic acid
Visit the PubMed ArticleRACK1 ... detected in the ... nucleus
Guo J, Wang S, Valerius O, Hall H, Zeng Q, Li JF, Weston DJ, Ellis BE, Chen JG - Involvement of Arabidopsis RACK1 in protein translation and its regulation by abscisic acid
Visit the PubMed ArticleTo gain further insights into the biochemical/molecular function of RACK1 in Arabidopsis, we performed global coexpression data analysis (PRIME; http://prime.psc.riken.jp/) to identify genes that are coexpressed with all three RACK1 genes. Surprisingly, we found that more than 80% (128 out of 154) of the genes that are coexpressed with RACK1 encode ribosomal proteins (Supplemental Fig. S3; Supplemental Table S3), implying a potential relationship between RACK1 function and the ribosome complex
Guo J, Wang S, Valerius O, Hall H, Zeng Q, Li JF, Weston DJ, Ellis BE, Chen JG - Involvement of Arabidopsis RACK1 in protein translation and its regulation by abscisic acid
Visit the PubMed ArticleUsing quantitative RT-PCR, we conducted a detailed analysis of the expression of RACK1 gene family members in response to ABA treatment. The level of transcripts for all three RACK1 genes was down-regulated as early as 1 h after ABA treatment and remained suppressed thereafter
Guo J, Wang S, Valerius O, Hall H, Zeng Q, Li JF, Weston DJ, Ellis BE, Chen JG - Involvement of Arabidopsis RACK1 in protein translation and its regulation by abscisic acid
Visit the PubMed ArticleReverse transcription (RT)-PCR analysis revealed that the expression of eIF6A is ubiquitous across various tissues and organs in Arabidopsis
Guo J, Wang S, Valerius O, Hall H, Zeng Q, Li JF, Weston DJ, Ellis BE, Chen JG - Involvement of Arabidopsis RACK1 in protein translation and its regulation by abscisic acid
Visit the PubMed ArticleWe then extended our analysis to examine the possible regulation of eIF6 expression by ABA. We found that a reduction of eIF6A expression could be detected as early as 15 min after ABA treatment, and expression of eIF6A continued to decline for up to 6 h
Guo J, Wang S, Valerius O, Hall H, Zeng Q, Li JF, Weston DJ, Ellis BE, Chen JG - Involvement of Arabidopsis RACK1 in protein translation and its regulation by abscisic acid
Visit the PubMed ArticleRACK1 ... detected in the cytoplasm
Guo J, Wang S, Valerius O, Hall H, Zeng Q, Li JF, Weston DJ, Ellis BE, Chen JG - Involvement of Arabidopsis RACK1 in protein translation and its regulation by abscisic acid
Visit the PubMed Articlein the RNase E mutants ... psbB ... a massive overaccumulation of unprocessed polycistronic precursor transcripts occurred
Walter M, Piepenburg K, Schöttler MA, Petersen K, Kahlau S, Tiller N, Drechsel O, Weingartner M, Kudla J, Bock R - Knockout of the plastid RNase E leads to defective RNA processing and chloroplast ribosome deficiency
Visit the PubMed ArticleThe rpoA transcript, encoding the a-subunit of the plastid-encoded RNA polymerase appears to be an exceptional case. Like most of the other genes analyzed, it displays overaccumulation of unprocessed precursors, but, in addition, it also shows strong accumulation of a shorter than normal RNA species (Figure 5). This RNA is much shorter than the reading frame of rpoA (approximately 1 kb) and therefore cannot represent a functional mRNA. It could either result from aberrant transcript processing and/or represent a degradation intermediate that is short lived in the wild type, but accumulates stably in the RNase E mutant
Walter M, Piepenburg K, Schöttler MA, Petersen K, Kahlau S, Tiller N, Drechsel O, Weingartner M, Kudla J, Bock R - Knockout of the plastid RNase E leads to defective RNA processing and chloroplast ribosome deficiency
Visit the PubMed Articlein the RNase E mutants ... 5' exon of the trans-spliced rps12 ... a massive overaccumulation of unprocessed polycistronic precursor transcripts occurred
Walter M, Piepenburg K, Schöttler MA, Petersen K, Kahlau S, Tiller N, Drechsel O, Weingartner M, Kudla J, Bock R - Knockout of the plastid RNase E leads to defective RNA processing and chloroplast ribosome deficiency
Visit the PubMed Articlein RNase E mutant plants ... Immunoblot analyses using antibodies against diagnostic subunits of the multiprotein complexes in the thylakoid membrane directly confirmed the reduced accumulation of both photosystems
Walter M, Piepenburg K, Schöttler MA, Petersen K, Kahlau S, Tiller N, Drechsel O, Weingartner M, Kudla J, Bock R - Knockout of the plastid RNase E leads to defective RNA processing and chloroplast ribosome deficiency
Visit the PubMed Articlein the rne/rne mutants ... rpl20 ... while showing a strong overaccumulation of precursors, did not display a reduction in the mature mRNA species
Walter M, Piepenburg K, Schöttler MA, Petersen K, Kahlau S, Tiller N, Drechsel O, Weingartner M, Kudla J, Bock R - Knockout of the plastid RNase E leads to defective RNA processing and chloroplast ribosome deficiency
Visit the PubMed Articlein the rne/rne mutants ... abnormal accumulation of precursor transcripts was seen for a tRNA gene, trnI-GAU, albeit accompanied with a less drastic reduction in the mature RNA
Walter M, Piepenburg K, Schöttler MA, Petersen K, Kahlau S, Tiller N, Drechsel O, Weingartner M, Kudla J, Bock R - Knockout of the plastid RNase E leads to defective RNA processing and chloroplast ribosome deficiency
Visit the PubMed ArticleTo confirm active transcription of the putative RNase E gene, RNA was extracted from whole plants, an Arabidopsis cell culture, young seedlings and leaf samples. Transcript accumulation was detectable in leaves but was undetectably low in all other samples (Figure 1c). When poly(A)+ RNA was analyzed instead of total cellular RNA strong hybridization signals were obtained, confirming that the RNase E gene is actively expressed, at least at the mRNA level
Walter M, Piepenburg K, Schöttler MA, Petersen K, Kahlau S, Tiller N, Drechsel O, Weingartner M, Kudla J, Bock R - Knockout of the plastid RNase E leads to defective RNA processing and chloroplast ribosome deficiency
Visit the PubMed Articlein the RNase E mutants ... ycf4 ... a massive overaccumulation of unprocessed polycistronic precursor transcripts occurred
Walter M, Piepenburg K, Schöttler MA, Petersen K, Kahlau S, Tiller N, Drechsel O, Weingartner M, Kudla J, Bock R - Knockout of the plastid RNase E leads to defective RNA processing and chloroplast ribosome deficiency
Visit the PubMed Articlein the RNase E mutants ... petA ... a massive overaccumulation of unprocessed polycistronic precursor transcripts occurred
Walter M, Piepenburg K, Schöttler MA, Petersen K, Kahlau S, Tiller N, Drechsel O, Weingartner M, Kudla J, Bock R - Knockout of the plastid RNase E leads to defective RNA processing and chloroplast ribosome deficiency
Visit the PubMed Articlein the rne/rne mutants ... clpP ... even showed a stronger accumulation of the mature RNA than in the wild type
Walter M, Piepenburg K, Schöttler MA, Petersen K, Kahlau S, Tiller N, Drechsel O, Weingartner M, Kudla J, Bock R - Knockout of the plastid RNase E leads to defective RNA processing and chloroplast ribosome deficiency
Visit the PubMed ArticleWe used this method to identify plastid genes, whose expression is most severely affected at the level of protein synthesis in the RNase E mutants (Figures 4 and S1b). Interestingly, when polysomal RNAs from wild-type plants and mutant plants were comparatively analyzed, a single gene was found to show drastically reduced association with ribosomes, namely rpl22. Significantly, rpl22 was much more strongly affected in the translatomics analysis than in the transcriptomics analysis (Figure 4a,b), tentatively suggesting that most of the unprocessed precursor transcripts accumulating in the mutant (Figure 5) are not translatable. Next, we analyzed the distribution of rpl22 transcripts across fractionated polysome gradients (Figure 6a). These experiments confirmed poor translation of the heterogeneous population of rpl22-containing transcripts present in the homozygous RNase E mutant
Walter M, Piepenburg K, Schöttler MA, Petersen K, Kahlau S, Tiller N, Drechsel O, Weingartner M, Kudla J, Bock R - Knockout of the plastid RNase E leads to defective RNA processing and chloroplast ribosome deficiency
Visit the PubMed ArticleThis was also apparent for psbB, a PSII gene, which is also part of a large polycistronic transcription unit (Westhoff and Herrmann, 1988). Accumulation of the mature psbB message was strongly reduced in the rne/rne mutants, while the unprocessed precursor transcript accumulated to higher levels than in the wild type
Walter M, Piepenburg K, Schöttler MA, Petersen K, Kahlau S, Tiller N, Drechsel O, Weingartner M, Kudla J, Bock R - Knockout of the plastid RNase E leads to defective RNA processing and chloroplast ribosome deficiency
Visit the PubMed ArticleAssays with different tissues confirmed the northern blot and GUS expression data (Figure 1e) and, in addition, revealed that expression of the Rne gene is ... suppressed by sucrose in the growth medium
Walter M, Piepenburg K, Schöttler MA, Petersen K, Kahlau S, Tiller N, Drechsel O, Weingartner M, Kudla J, Bock R - Knockout of the plastid RNase E leads to defective RNA processing and chloroplast ribosome deficiency
Visit the PubMed ArticleTwo independently generated RNAi lines that both show a reduction of RPL24 expression levels to between 15 and 20% of wild-type levels were analyzed ... Processing of polycistronic transcripts was not defective, and ... the mature petA mRNA accumulated to similar levels to those in the wild type
Walter M, Piepenburg K, Schöttler MA, Petersen K, Kahlau S, Tiller N, Drechsel O, Weingartner M, Kudla J, Bock R - Knockout of the plastid RNase E leads to defective RNA processing and chloroplast ribosome deficiency
Visit the PubMed ArticleAssays with different tissues confirmed the northern blot and GUS expression data (Figure 1e) and, in addition, revealed that expression of the Rne gene is strongly elevated in the dark
Walter M, Piepenburg K, Schöttler MA, Petersen K, Kahlau S, Tiller N, Drechsel O, Weingartner M, Kudla J, Bock R - Knockout of the plastid RNase E leads to defective RNA processing and chloroplast ribosome deficiency
Visit the PubMed Articlehomozygous RNase E knockout plants showed a moderate reduction in their content of small subunits of the chloroplast ribosome (determined as the ratio 18S rRNA:16S rRNA), but a more pronounced reduction in the large subunit of the chloroplast ribosome (determined as the ratio 18S rRNA:23S rRNA and the ratio 16S rRNA:23S rRNA; Figure 6b). The data revealed that the homozygous mutants had fewer than 50% of the chloroplast large ribosomal subunits compared with the wild type (yellow bars in Figure 6b), whereas the heterozygous mutant was not significantly different from the wild type. These findings support the assumption that defective rpl22 processing causes plastid ribosome deficiency in homozygous RNase E knockout plants
Walter M, Piepenburg K, Schöttler MA, Petersen K, Kahlau S, Tiller N, Drechsel O, Weingartner M, Kudla J, Bock R - Knockout of the plastid RNase E leads to defective RNA processing and chloroplast ribosome deficiency
Visit the PubMed ArticleTo confirm the exclusive localization in chloroplasts and exclude dual targeting to chloroplasts and mitochondria, we translationally fused the sequence encoding the N-terminal 82 amino acids of RNase E to the gene for the green fluorescence protein (GFP) and introduced this construct into the nuclear genome of Arabidopsis by Agrobacterium tumefaciens-mediated transformation. Analysis of the subcellular localization of GFP fluorescence in cells of the generated stable transgenic plants suggests that the Arabidopsis RNase E is targeted exclusively to chloroplasts
Walter M, Piepenburg K, Schöttler MA, Petersen K, Kahlau S, Tiller N, Drechsel O, Weingartner M, Kudla J, Bock R - Knockout of the plastid RNase E leads to defective RNA processing and chloroplast ribosome deficiency
Visit the PubMed Articlein the rne/rne mutants ... rpl23 ... while showing a strong overaccumulation of precursors, did not display a reduction in the mature mRNA species
Walter M, Piepenburg K, Schöttler MA, Petersen K, Kahlau S, Tiller N, Drechsel O, Weingartner M, Kudla J, Bock R - Knockout of the plastid RNase E leads to defective RNA processing and chloroplast ribosome deficiency
Visit the PubMed ArticleMature rbcL ... mRNAs accumulated to very similar levels in the wild type, heterozygous RNase E mutants and homozygous RNase E mutants ... suggesting that RNase E does not contribute significantly to controlling the mRNA levels of these genes, at least not under photoautotrophic growth conditions in soil
Walter M, Piepenburg K, Schöttler MA, Petersen K, Kahlau S, Tiller N, Drechsel O, Weingartner M, Kudla J, Bock R - Knockout of the plastid RNase E leads to defective RNA processing and chloroplast ribosome deficiency
Visit the PubMed ArticleWhen the levels of polyadenylated transcripts of rbcL ... were determined by quantitative (q)RT-PCR (see Experimental Procedures), they were found to be generally very low (Cp values of ... 29 cycles for rbcL ... This is consistent with previous data and the notion that polyadenylated mRNAs represent short-lived degradation intermediates (Kudla et al., 1996; Lisitsky et al., 1996). No significant differences were observed between the RNase E mutant and the wild type
Walter M, Piepenburg K, Schöttler MA, Petersen K, Kahlau S, Tiller N, Drechsel O, Weingartner M, Kudla J, Bock R - Knockout of the plastid RNase E leads to defective RNA processing and chloroplast ribosome deficiency
Visit the PubMed Articleputative RNase E gene ... The GUS staining of transgenic plants revealed strong expression in green (chloroplast-containing) tissues, including cotyledons, true leaves and sepals with expression in mature (fully expanded) leaves being much stronger than expression in young developing leaves (Figure 1d). In contrast, expression was undetectably low in most non-green tissues, including roots, petals and stamens
Walter M, Piepenburg K, Schöttler MA, Petersen K, Kahlau S, Tiller N, Drechsel O, Weingartner M, Kudla J, Bock R - Knockout of the plastid RNase E leads to defective RNA processing and chloroplast ribosome deficiency
Visit the PubMed ArticleWhen the levels of polyadenylated transcripts of ... psbA ... were determined by quantitative (q)RT-PCR (see Experimental Procedures), they were found to be generally very low (Cp values of 34–35 cycles for psbA ... This is consistent with previous data and the notion that polyadenylated mRNAs represent short-lived degradation intermediates (Kudla et al., 1996; Lisitsky et al., 1996). No significant differences were observed between the RNase E mutant and the wild type
Walter M, Piepenburg K, Schöttler MA, Petersen K, Kahlau S, Tiller N, Drechsel O, Weingartner M, Kudla J, Bock R - Knockout of the plastid RNase E leads to defective RNA processing and chloroplast ribosome deficiency
Visit the PubMed Articlewe quantified cytosolic and chloroplast ribosomes in fractionated polysome gradients. The results clearly show that ribosome coverage of chloroplast mRNAs is much less dense in RNase E mutants, as evidenced by the strong decline in the 16S rRNA : 18S rRNA ratio with increasing sucrose density in the gradient and undetectably low levels of plastid ribosomes in the bottom fractions of the gradient (Figure 6c). As expected, coverage of cytosolic mRNAs with ribosomes is unaffected in the RNase E knockout plants
Walter M, Piepenburg K, Schöttler MA, Petersen K, Kahlau S, Tiller N, Drechsel O, Weingartner M, Kudla J, Bock R - Knockout of the plastid RNase E leads to defective RNA processing and chloroplast ribosome deficiency
Visit the PubMed Articlein the RNase E mutants ... psaI ... a massive overaccumulation of unprocessed polycistronic precursor transcripts occurred
Walter M, Piepenburg K, Schöttler MA, Petersen K, Kahlau S, Tiller N, Drechsel O, Weingartner M, Kudla J, Bock R - Knockout of the plastid RNase E leads to defective RNA processing and chloroplast ribosome deficiency
Visit the PubMed ArticleMature ... psbA mRNAs accumulated to very similar levels in the wild type, heterozygous RNase E mutants and homozygous RNase E mutants ... suggesting that RNase E does not contribute significantly to controlling the mRNA levels of these genes, at least not under photoautotrophic growth conditions in soil
Walter M, Piepenburg K, Schöttler MA, Petersen K, Kahlau S, Tiller N, Drechsel O, Weingartner M, Kudla J, Bock R - Knockout of the plastid RNase E leads to defective RNA processing and chloroplast ribosome deficiency
Visit the PubMed Articlethe gene for the cytochrome f subunit of the cytochrome b6f complex, petA, and the PSI genes psaI and ycf4 are part of the same operon that is transcribed as a large polycistronic precursor transcript of approximately 8 kb. This precursor transcript also includes the upstream rbcL gene (Figure S2), indicating that it originates from the rbcL promoter and spans the rbcL, accD, psaI, ycf4, ycf10 and petA genes, which all have the same transcriptional orientation (Figure S1). The 8 kb precursor RNA massively overaccumulates in homozygous RNase E mutants
Walter M, Piepenburg K, Schöttler MA, Petersen K, Kahlau S, Tiller N, Drechsel O, Weingartner M, Kudla J, Bock R - Knockout of the plastid RNase E leads to defective RNA processing and chloroplast ribosome deficiency
Visit the PubMed Articlethe mRNA most strongly affected by the knockout of RNase E is rpl22, an essential ribosomal protein of the large subunit of the chloroplast ribosome (Figure 5). In the homozygous mutants, mature rpl22 mRNA is hardly detectable and, instead, very long precursor RNA species (>8 kb) accumulate. This raises the possibility that the mutant phenotype of the rne/rne plants (Figure 2) is largely due to plastid ribosome deficiency
Walter M, Piepenburg K, Schöttler MA, Petersen K, Kahlau S, Tiller N, Drechsel O, Weingartner M, Kudla J, Bock R - Knockout of the plastid RNase E leads to defective RNA processing and chloroplast ribosome deficiency
Visit the PubMed ArticleTwo independently generated RNAi lines that both show a reduction of RPL24 expression levels to between 15 and 20% of wild-type levels were analyzed ... Processing of polycistronic transcripts was not defective, and ... the mature psbB mRNA ... accumulated to similar levels to those in the wild type
Walter M, Piepenburg K, Schöttler MA, Petersen K, Kahlau S, Tiller N, Drechsel O, Weingartner M, Kudla J, Bock R - Knockout of the plastid RNase E leads to defective RNA processing and chloroplast ribosome deficiency
Visit the PubMed ArticleWhen the levels of polyadenylated transcripts of ... rps14 were determined by quantitative (q)RT-PCR (see Experimental Procedures), they were found to be generally very low (Cp values of 34–35 cycles for ... rps14 ... This is consistent with previous data and the notion that polyadenylated mRNAs represent short-lived degradation intermediates (Kudla et al., 1996; Lisitsky et al., 1996). No significant differences were observed between the RNase E mutant and the wild type
Walter M, Piepenburg K, Schöttler MA, Petersen K, Kahlau S, Tiller N, Drechsel O, Weingartner M, Kudla J, Bock R - Knockout of the plastid RNase E leads to defective RNA processing and chloroplast ribosome deficiency
Visit the PubMed Articlelow accumulation levels of the psbE operon transcript in the RNase E mutant plants ... The psbE operon comprises the four small PSII genes psbE, psbF, psbL and psbJ and is transcribed as a tetracistronic mRNA that remains tetracistronic and does not undergo cleavage into monocistronic units (Willey and Gray, 1989). Disturbed plastid gene expression has been shown to result in reduced psbE operon transcription and RNA accumulation
Walter M, Piepenburg K, Schöttler MA, Petersen K, Kahlau S, Tiller N, Drechsel O, Weingartner M, Kudla J, Bock R - Knockout of the plastid RNase E leads to defective RNA processing and chloroplast ribosome deficiency
Visit the PubMed Articlein the RNase E mutants ... rpl20 ... massive overaccumulation of unprocessed polycistronic precursor transcripts occurred
Walter M, Piepenburg K, Schöttler MA, Petersen K, Kahlau S, Tiller N, Drechsel O, Weingartner M, Kudla J, Bock R - Knockout of the plastid RNase E leads to defective RNA processing and chloroplast ribosome deficiency
Visit the PubMed Articlein the rne/rne mutants ... trnR-ACG ... even showed a stronger accumulation of the mature RNA than in the wild type
Walter M, Piepenburg K, Schöttler MA, Petersen K, Kahlau S, Tiller N, Drechsel O, Weingartner M, Kudla J, Bock R - Knockout of the plastid RNase E leads to defective RNA processing and chloroplast ribosome deficiency
Visit the PubMed Articlein the RNase E mutants ... rpl23 ... a massive overaccumulation of unprocessed polycistronic precursor transcripts occurred
Walter M, Piepenburg K, Schöttler MA, Petersen K, Kahlau S, Tiller N, Drechsel O, Weingartner M, Kudla J, Bock R - Knockout of the plastid RNase E leads to defective RNA processing and chloroplast ribosome deficiency
Visit the PubMed ArticleSHR belongs to a small clade of GRAS genes that includes SCARECROW-LIKE29 (SCL29) and SCL32 (Bolle, 2004; Lee et al., 2008). Therefore, we asked whether SCL29 and SCL32 were expressed in the leaf in a pattern similar to that of SHR. To address this question, we visualized expression of transcriptional and translational fusions of SCL29 or SCL32 to YFP in 4-DAG first leaves ... activity of SCL32 fusions was detected at nearly all subepidermal positions
Gardiner J, Donner TJ, Scarpella E - Simultaneous activation of SHR and ATHB8 expression defines switch to preprocambial cell state in Arabidopsis leaf development
Visit the PubMed ArticleExpression of SHR in second loops of 4-DAG leaves (Fig. 2H; compare with Fig. 1D and Fig. 2E), suggests that, like ATHB8 (Kang and Dengler, 2004; Scarpella et al., 2004), SHR is expressed in ground cells that have shifted to preprocambial state
Gardiner J, Donner TJ, Scarpella E - Simultaneous activation of SHR and ATHB8 expression defines switch to preprocambial cell state in Arabidopsis leaf development
Visit the PubMed ArticleAt 2 DAG ... ATHB8::HTA6:EYFP signals were confined to a single cell file along the midline of the leaf primordium (Fig. 3A,E). At 3 DAG ... ATHB8 transcriptional fusions were expressed in narrow domains at sites of midvein and first loop formation ... At 4 DAG, slender zones of ... ATHB8::HTA6:EYFP activity marked appearance of midvein, first and second loops, and higher-order veins
Gardiner J, Donner TJ, Scarpella E - Simultaneous activation of SHR and ATHB8 expression defines switch to preprocambial cell state in Arabidopsis leaf development
Visit the PubMed ArticleFinally, at 5 DAG, SHR ... promoters directed expression in ... higher-order veins
Gardiner J, Donner TJ, Scarpella E - Simultaneous activation of SHR and ATHB8 expression defines switch to preprocambial cell state in Arabidopsis leaf development
Visit the PubMed ArticleFinally, at 5 DAG, SHR ... promoters directed expression in ... first, second, and third loops ... veins
Gardiner J, Donner TJ, Scarpella E - Simultaneous activation of SHR and ATHB8 expression defines switch to preprocambial cell state in Arabidopsis leaf development
Visit the PubMed ArticleFinally, at 5 DAG ... ATHB8 promoters directed expression in developing midvein
Gardiner J, Donner TJ, Scarpella E - Simultaneous activation of SHR and ATHB8 expression defines switch to preprocambial cell state in Arabidopsis leaf development
Visit the PubMed ArticleFinally, at 5 DAG, SHR ... promoters directed expression in developing midvein
Gardiner J, Donner TJ, Scarpella E - Simultaneous activation of SHR and ATHB8 expression defines switch to preprocambial cell state in Arabidopsis leaf development
Visit the PubMed ArticleFinally, at 5 DAG ... ATHB8 promoters directed expression in ... first, second, and third loops ... veins
Gardiner J, Donner TJ, Scarpella E - Simultaneous activation of SHR and ATHB8 expression defines switch to preprocambial cell state in Arabidopsis leaf development
Visit the PubMed ArticleFinally, at 5 DAG ... ATHB8 promoters directed expression in ... higher-order veins
Gardiner J, Donner TJ, Scarpella E - Simultaneous activation of SHR and ATHB8 expression defines switch to preprocambial cell state in Arabidopsis leaf development
Visit the PubMed ArticleSHR belongs to a small clade of GRAS genes that includes SCARECROW-LIKE29 (SCL29) and SCL32 (Bolle, 2004; Lee et al., 2008). Therefore, we asked whether SCL29 and SCL32 were expressed in the leaf in a pattern similar to that of SHR. To address this question, we visualized expression of transcriptional and translational fusions of SCL29 or SCL32 to YFP in 4-DAG first leaves ... While expression of SCL29 fusions was confined to epidermal cells
Gardiner J, Donner TJ, Scarpella E - Simultaneous activation of SHR and ATHB8 expression defines switch to preprocambial cell state in Arabidopsis leaf development
Visit the PubMed Articlewe constructed a translational fusion of GFP to the 5′-end of the genomic SYP23Col-0 sequence (SYP23g; Figure 6a) and introduced it into syp22-3 syp23. GFP-SYP23Col-0 complemented the defect in syp23 (Figure S4), indicating that GFP–SYP23Col-0 is functional. Subcellular fractionation followed by immunoblot analysis showed that GFP–SYP23Col-0 was present in the supernatant fraction (S100) after ultracentrifugation at 100 000 g (Figure 6b), suggesting that most GFP–SYP23Col-0 is not associated with the membrane. Consistent with this result, GFP fluorescence of GFP–SYP23Col-0 was observed in the cytosol of leaf cells (Figure 6c,d) and root cells (Figure 6e,f). In addition, we observed the same subcellular localization of SYP23 by using both the SYP23Col-0 cDNA fused to the 5′-end of GFP (SYP23Col-0-GFP ) (Figure 6g) and the genomic sequence fused to the 3′-end of mRFP (mRFP-SYP23Col-0) (Figure S5). mRFP–SYP23Col-0 complemented the defect in syp23 (Figure S6), indicating that mRFP–SYP23Col-0 is functional. These results suggest that SYP23 is localized in the cytosol
Shirakawa M, Ueda H, Shimada T, Koumoto Y, Shimada TL, Kondo M, Takahashi T, Okuyama Y, Nishimura M, Hara-Nishimura I - Arabidopsis Qa-SNARE SYP2 proteins localized to different subcellular regions function redundantly in vacuolar protein sorting and plant development
Visit the PubMed Articlesyp21amisyp22-3 ... accumulated much higher levels of TGG1 than did single mutants or syp21 syp23
Shirakawa M, Ueda H, Shimada T, Koumoto Y, Shimada TL, Kondo M, Takahashi T, Okuyama Y, Nishimura M, Hara-Nishimura I - Arabidopsis Qa-SNARE SYP2 proteins localized to different subcellular regions function redundantly in vacuolar protein sorting and plant development
Visit the PubMed ArticleThe broad expression patterns of SYP21 and SYP23 were similar to that of SYP22
Shirakawa M, Ueda H, Shimada T, Koumoto Y, Shimada TL, Kondo M, Takahashi T, Okuyama Y, Nishimura M, Hara-Nishimura I - Arabidopsis Qa-SNARE SYP2 proteins localized to different subcellular regions function redundantly in vacuolar protein sorting and plant development
Visit the PubMed Articlesyp21amisyp22-1 ... accumulated much higher levels of TGG1 than did single mutants or syp21 syp23
Shirakawa M, Ueda H, Shimada T, Koumoto Y, Shimada TL, Kondo M, Takahashi T, Okuyama Y, Nishimura M, Hara-Nishimura I - Arabidopsis Qa-SNARE SYP2 proteins localized to different subcellular regions function redundantly in vacuolar protein sorting and plant development
Visit the PubMed ArticleThe intensity of GUS staining was slightly higher in proSYP21:GUS plants than in proSYP23:GUS plants
Shirakawa M, Ueda H, Shimada T, Koumoto Y, Shimada TL, Kondo M, Takahashi T, Okuyama Y, Nishimura M, Hara-Nishimura I - Arabidopsis Qa-SNARE SYP2 proteins localized to different subcellular regions function redundantly in vacuolar protein sorting and plant development
Visit the PubMed Articlesyp22 syp23 ... accumulated much higher levels of TGG1 than did single mutants or syp21 syp23
Shirakawa M, Ueda H, Shimada T, Koumoto Y, Shimada TL, Kondo M, Takahashi T, Okuyama Y, Nishimura M, Hara-Nishimura I - Arabidopsis Qa-SNARE SYP2 proteins localized to different subcellular regions function redundantly in vacuolar protein sorting and plant development
Visit the PubMed ArticleThe broad expression patterns of SYP21 and SYP23 were similar to that of SYP22
Shirakawa M, Ueda H, Shimada T, Koumoto Y, Shimada TL, Kondo M, Takahashi T, Okuyama Y, Nishimura M, Hara-Nishimura I - Arabidopsis Qa-SNARE SYP2 proteins localized to different subcellular regions function redundantly in vacuolar protein sorting and plant development
Visit the PubMed Articlethe triple mutant syp21amisyp22-1 syp23 accumulated much higher levels of TGG1 than did single mutants or syp21 syp23
Shirakawa M, Ueda H, Shimada T, Koumoto Y, Shimada TL, Kondo M, Takahashi T, Okuyama Y, Nishimura M, Hara-Nishimura I - Arabidopsis Qa-SNARE SYP2 proteins localized to different subcellular regions function redundantly in vacuolar protein sorting and plant development
Visit the PubMed ArticleThe intensity of GUS staining was slightly higher in proSYP21:GUS plants than in proSYP23:GUS plants
Shirakawa M, Ueda H, Shimada T, Koumoto Y, Shimada TL, Kondo M, Takahashi T, Okuyama Y, Nishimura M, Hara-Nishimura I - Arabidopsis Qa-SNARE SYP2 proteins localized to different subcellular regions function redundantly in vacuolar protein sorting and plant development
Visit the PubMed ArticleThe broad expression patterns of SYP21 and SYP23 were similar to that of SYP22
Shirakawa M, Ueda H, Shimada T, Koumoto Y, Shimada TL, Kondo M, Takahashi T, Okuyama Y, Nishimura M, Hara-Nishimura I - Arabidopsis Qa-SNARE SYP2 proteins localized to different subcellular regions function redundantly in vacuolar protein sorting and plant development
Visit the PubMed Articlein the apum23-1 mutant ... Hybridization of total RNA using probes specific for 5'ETS + 18S, 18S, ITS1, 5.8S, ITS2 and 25S rRNAs, respectively, resulted in detection of a higher level of 35S pre-rRNA
Abbasi N, Kim HB, Park NI, Kim HS, Kim YK, Park YI, Choi SB - APUM23, a nucleolar Puf domain protein, is involved in pre-ribosomal RNA processing and normal growth patterning in Arabidopsis
Visit the PubMed ArticleIn apum23-1, we found a 281 nt polyadenylated pre-rRNA that contained 5.8S rRNA (164 nt) and ITS2 (117 nt, ending at the sequence CGGTGG), in addition to a poly(A)12–18 tail (Figure 6c). However, we did not detect a mis-processed 5' end of the 281 nt pre-rRNA using circular RT-PCR
Abbasi N, Kim HB, Park NI, Kim HS, Kim YK, Park YI, Choi SB - APUM23, a nucleolar Puf domain protein, is involved in pre-ribosomal RNA processing and normal growth patterning in Arabidopsis
Visit the PubMed ArticleThis result was verified by a GUS histochemical assay using PAPUM23:GUS transgenic plants. GUS staining of homozygous T3 transgenic plants indicated that APUM23 is ... expressed ... in rapidly expanding tissues such as cotyledons of germinating seedlings
Abbasi N, Kim HB, Park NI, Kim HS, Kim YK, Park YI, Choi SB - APUM23, a nucleolar Puf domain protein, is involved in pre-ribosomal RNA processing and normal growth patterning in Arabidopsis
Visit the PubMed Articleidentified in the microarray data ... AtNuc-L2 was greatly up-regulated in apum23-1 plants
Abbasi N, Kim HB, Park NI, Kim HS, Kim YK, Park YI, Choi SB - APUM23, a nucleolar Puf domain protein, is involved in pre-ribosomal RNA processing and normal growth patterning in Arabidopsis
Visit the PubMed Articlewhen hybridization was performed using poly(A) RNA, a band at approximately 2.6 kb was detected in blots for 5'ETS + 18S, 18S and ITS1 fragment-specific probes (Figure 5b,c; indicated by a white asterisk), but not for 5.8S, ITS2, 25S and 3'ETS probes, suggesting that the apum23-1 mutant is impaired in degradation of polyadenylated maturation by-products
Abbasi N, Kim HB, Park NI, Kim HS, Kim YK, Park YI, Choi SB - APUM23, a nucleolar Puf domain protein, is involved in pre-ribosomal RNA processing and normal growth patterning in Arabidopsis
Visit the PubMed Articlewe assessed the localization of a C-terminal APUM23–GFP translational fusion protein in the root cells of transgenic Arabidopsis ... Confocal microscopy showed that the GFP signal was localized to the nucleolus; it was distinct from the AtCoilin localization and surrounded by DAPI fluorescence ... indicating that APUM23–GFP is localized to the nucleolus. To confirm that the GFP fluorescence emanated from the nucleolus, we examined tobacco leaf cells transiently expressing APUM23–GFP and mRFP–AtFibrillarin2 (mRFP–AtFib2) fusion proteins simultaneously (Figure 4g–i). Confocal imaging showed co-localization of GFP and RFP signals in the nucleolus, but not in the Cajal body. As fibrillarin localizes to both the nucleolus and the Cajal body while, Coilin resides only in the Cajal body, it is clear that APUM23–GFP localized to the nucleolus
Abbasi N, Kim HB, Park NI, Kim HS, Kim YK, Park YI, Choi SB - APUM23, a nucleolar Puf domain protein, is involved in pre-ribosomal RNA processing and normal growth patterning in Arabidopsis
Visit the PubMed ArticleThis result was verified by a GUS histochemical assay using PAPUM23:GUS transgenic plants. GUS staining of homozygous T3 transgenic plants indicated that APUM23 is ... expressed ... in the early developing leaf
Abbasi N, Kim HB, Park NI, Kim HS, Kim YK, Park YI, Choi SB - APUM23, a nucleolar Puf domain protein, is involved in pre-ribosomal RNA processing and normal growth patterning in Arabidopsis
Visit the PubMed Articleno expression of APUM23 was seen in fully expanded cotyledons
Abbasi N, Kim HB, Park NI, Kim HS, Kim YK, Park YI, Choi SB - APUM23, a nucleolar Puf domain protein, is involved in pre-ribosomal RNA processing and normal growth patterning in Arabidopsis
Visit the PubMed Articleno expression of APUM23 was seen in fully expanded ... leaves
Abbasi N, Kim HB, Park NI, Kim HS, Kim YK, Park YI, Choi SB - APUM23, a nucleolar Puf domain protein, is involved in pre-ribosomal RNA processing and normal growth patterning in Arabidopsis
Visit the PubMed Articlewe examined the transcriptional expression pattern of APUM23 in wild-type Col-0 plants by quantitative RT-PCR. APUM23 was expressed in all organs tested, including leaves, flowers, stems, siliques and roots
Abbasi N, Kim HB, Park NI, Kim HS, Kim YK, Park YI, Choi SB - APUM23, a nucleolar Puf domain protein, is involved in pre-ribosomal RNA processing and normal growth patterning in Arabidopsis
Visit the PubMed ArticleWe therefore examined APUM23 expression in wild-type Col-0 seedlings treated with 3% glucose and 5% sucrose ... expression of APUM23 was increased within 30 min, and the elevated expression level was maintained for up to 6 h. This suggests that APUM23 expression is dependent on the nutrient supply in metabolically active tissues. This result was also confirmed by supplementation of PAPUM23:GUS transgenic plants with either glucose
Abbasi N, Kim HB, Park NI, Kim HS, Kim YK, Park YI, Choi SB - APUM23, a nucleolar Puf domain protein, is involved in pre-ribosomal RNA processing and normal growth patterning in Arabidopsis
Visit the PubMed ArticleCompared to wild-type, apum23-1 showed accumulation of the unprocessed form of 18S rRNA (Figure 6b, upper panel). The bands were cloned and sequenced, and were found to include the following: the 5'ETS (563 nt from the P site to the 5' end of mature 18S rRNA), part of 18S rRNA depending on the primer position (204 nt from the 5' end of 18S rRNA to the position of primer 18S cRT-PCR F and 56 nt from primer 18S cRT-PCR R to the 3' end of the 18S rRNA), part of ITS1 containing 192 nt ending at the conserved site AAGGAAC, and 15–33 nt long poly(A) tails (Figure 6b, lower panel)
Abbasi N, Kim HB, Park NI, Kim HS, Kim YK, Park YI, Choi SB - APUM23, a nucleolar Puf domain protein, is involved in pre-ribosomal RNA processing and normal growth patterning in Arabidopsis
Visit the PubMed ArticleNo difference was detected between wild-type and apum23-1 for band pattern or intensity in the 5S rRNA blot (Figure 6c), suggesting that 5S rRNA processing is not affected in the apum23-1 mutant
Abbasi N, Kim HB, Park NI, Kim HS, Kim YK, Park YI, Choi SB - APUM23, a nucleolar Puf domain protein, is involved in pre-ribosomal RNA processing and normal growth patterning in Arabidopsis
Visit the PubMed ArticleAtHAM1 expression is maintained in differentiating stem provasculature
Engstrom EM, Andersen CM, Gumulak-Smith J, Hu J, Orlova E, Sozzani R, Bowman JL - Arabidopsis homologs of the petunia hairy meristem gene are required for maintenance of shoot and root indeterminacy
Visit the PubMed Articlewe performed in situ hybridization experiments to determine the localization of AtHAM1 expression in shoot apices of 12-d-old wild-type seedlings. In situ hybridization reveals the expression of AtHAM1 in ... differentiating leaf primordia, with the level of expression elevated in interior regions of leaf primordia
Engstrom EM, Andersen CM, Gumulak-Smith J, Hu J, Orlova E, Sozzani R, Bowman JL - Arabidopsis homologs of the petunia hairy meristem gene are required for maintenance of shoot and root indeterminacy
Visit the PubMed Articlewe undertook to amplify segments of HAM orthologs AtHAM1, AtHAM2, and AtHAM3 by reverse transcription (RT)-PCR from cDNA derived from entire vegetative shoots ... using primer sets that discriminate full-length transcripts from MIR170/171 cleavage products ... AtHAM2 ... consistently amplified from all tissues surveyed
Engstrom EM, Andersen CM, Gumulak-Smith J, Hu J, Orlova E, Sozzani R, Bowman JL - Arabidopsis homologs of the petunia hairy meristem gene are required for maintenance of shoot and root indeterminacy
Visit the PubMed ArticleWe identified insertional mutant alleles, predicted to confer complete loss of function ... AtHAM2 ... Consistent with the insertions generating null loss-of-function (knockout) alleles, wild-type transcripts are not detectable by RT-PCR in homozygous insertion allele backgrounds
Engstrom EM, Andersen CM, Gumulak-Smith J, Hu J, Orlova E, Sozzani R, Bowman JL - Arabidopsis homologs of the petunia hairy meristem gene are required for maintenance of shoot and root indeterminacy
Visit the PubMed Articlewe undertook to amplify segments of HAM orthologs AtHAM1, AtHAM2, and AtHAM3 by reverse transcription (RT)-PCR from cDNA derived from entire vegetative shoots ... using primer sets that discriminate full-length transcripts from MIR170/171 cleavage products. AtHAM1 ... consistently amplified from all tissues surveyed
Engstrom EM, Andersen CM, Gumulak-Smith J, Hu J, Orlova E, Sozzani R, Bowman JL - Arabidopsis homologs of the petunia hairy meristem gene are required for maintenance of shoot and root indeterminacy
Visit the PubMed ArticleWithin the shoot meristem, AtHAM1 expression exhibits a gradient of expression ... increasing expression progressing downward into L3 meristem cells
Engstrom EM, Andersen CM, Gumulak-Smith J, Hu J, Orlova E, Sozzani R, Bowman JL - Arabidopsis homologs of the petunia hairy meristem gene are required for maintenance of shoot and root indeterminacy
Visit the PubMed Articlewe undertook to amplify segments of HAM orthologs AtHAM1, AtHAM2, and AtHAM3 by reverse transcription (RT)-PCR from cDNA derived from ... mature rosette leaves ... using primer sets that discriminate full-length transcripts from MIR170/171 cleavage products ... AtHAM2 ... consistently amplified from all tissues surveyed
Engstrom EM, Andersen CM, Gumulak-Smith J, Hu J, Orlova E, Sozzani R, Bowman JL - Arabidopsis homologs of the petunia hairy meristem gene are required for maintenance of shoot and root indeterminacy
Visit the PubMed Articlebasal of the shoot meristem boundary, there is a sharp decrease or cessation in AtHAM1 expression
Engstrom EM, Andersen CM, Gumulak-Smith J, Hu J, Orlova E, Sozzani R, Bowman JL - Arabidopsis homologs of the petunia hairy meristem gene are required for maintenance of shoot and root indeterminacy
Visit the PubMed ArticleWe identified insertional mutant alleles, predicted to confer complete loss of function ... AtHAM3 ... Consistent with the insertions generating null loss-of-function (knockout) alleles, wild-type transcripts are not detectable by RT-PCR in homozygous insertion allele backgrounds
Engstrom EM, Andersen CM, Gumulak-Smith J, Hu J, Orlova E, Sozzani R, Bowman JL - Arabidopsis homologs of the petunia hairy meristem gene are required for maintenance of shoot and root indeterminacy
Visit the PubMed Articlewe performed in situ hybridization experiments to determine the localization of AtHAM1 expression in shoot apices of 12-d-old wild-type seedlings. In situ hybridization reveals the expression of AtHAM1 in young leaves
Engstrom EM, Andersen CM, Gumulak-Smith J, Hu J, Orlova E, Sozzani R, Bowman JL - Arabidopsis homologs of the petunia hairy meristem gene are required for maintenance of shoot and root indeterminacy
Visit the PubMed Articlewe undertook to amplify segments of HAM orthologs AtHAM1, AtHAM2, and AtHAM3 by reverse transcription (RT)-PCR from cDNA derived from ... mature rosette leaves ... using primer sets that discriminate full-length transcripts from MIR170/171 cleavage products ... AtHAM1 ... consistently amplified from all tissues surveyed
Engstrom EM, Andersen CM, Gumulak-Smith J, Hu J, Orlova E, Sozzani R, Bowman JL - Arabidopsis homologs of the petunia hairy meristem gene are required for maintenance of shoot and root indeterminacy
Visit the PubMed ArticleWe identified insertional mutant alleles, predicted to confer complete loss of function, for AtHAM1 ... Consistent with the insertions generating null loss-of-function (knockout) alleles, wild-type transcripts are not detectable by RT-PCR in homozygous insertion allele backgrounds
Engstrom EM, Andersen CM, Gumulak-Smith J, Hu J, Orlova E, Sozzani R, Bowman JL - Arabidopsis homologs of the petunia hairy meristem gene are required for maintenance of shoot and root indeterminacy
Visit the PubMed Articlewe undertook to amplify segments of HAM orthologs AtHAM1, AtHAM2, and AtHAM3 by reverse transcription (RT)-PCR from cDNA derived from ... mature rosette leaves ... using primer sets that discriminate full-length transcripts from MIR170/171 cleavage products ... AtHAM3 are consistently amplified from all tissues surveyed
Engstrom EM, Andersen CM, Gumulak-Smith J, Hu J, Orlova E, Sozzani R, Bowman JL - Arabidopsis homologs of the petunia hairy meristem gene are required for maintenance of shoot and root indeterminacy
Visit the PubMed ArticleWithin the shoot meristem, AtHAM1 expression exhibits a gradient of expression, with the lowest level of expression in the meristem L1 layer
Engstrom EM, Andersen CM, Gumulak-Smith J, Hu J, Orlova E, Sozzani R, Bowman JL - Arabidopsis homologs of the petunia hairy meristem gene are required for maintenance of shoot and root indeterminacy
Visit the PubMed Articlewe undertook to amplify segments of HAM orthologs AtHAM1, AtHAM2, and AtHAM3 by reverse transcription (RT)-PCR from cDNA derived from entire vegetative shoots ... using primer sets that discriminate full-length transcripts from MIR170/171 cleavage products ... AtHAM3 are consistently amplified from all tissues surveyed
Engstrom EM, Andersen CM, Gumulak-Smith J, Hu J, Orlova E, Sozzani R, Bowman JL - Arabidopsis homologs of the petunia hairy meristem gene are required for maintenance of shoot and root indeterminacy
Visit the PubMed Articlethe expression ... was down-regulated ... Overexpression of PAR1-G or truncated AHC-G and HC-G also had very similar effects on the auxin-induced levels of transcripts encoding SAUR15
Galstyan A, Cifuentes-Esquivel N, Bou-Torrent J, Martinez-Garcia JF - The shade avoidance syndrome in Arabidopsis: a fundamental role for atypical basic helix-loop-helix proteins as transcriptional cofactors
Visit the PubMed Articlewe investigated whether PAR1 could be converted into a transcriptional activator by fusing it to the transactivating domain of the herpes viral protein VP16 (VP16-AD). Despite the non-plant origin of this protein, fusions of VP16-AD to plant DNA-binding transcription factors have been successfully used to convert transcriptional repressors into activators (Steindler et al., 1999; Parcy et al., 2002; Sawa et al., 2002). In the absence of direct or indirect access to DNA regulatory sequences, overexpression of PAR1 fused to VP16-AD (P35S:PAR1-V lines, Figure 3a) was expected to result in a phenotype virtually identical to that observed by overexpressing a wild-type version of PAR1. In agreement, P35S:PAR1-V lines ... simulated shade-induced SAUR15 ... expression
Galstyan A, Cifuentes-Esquivel N, Bou-Torrent J, Martinez-Garcia JF - The shade avoidance syndrome in Arabidopsis: a fundamental role for atypical basic helix-loop-helix proteins as transcriptional cofactors
Visit the PubMed ArticleAll variants containing the N-terminal region of PAR1 (N-G, NA-G and NAH-G) were mainly localized in the nuclei
Galstyan A, Cifuentes-Esquivel N, Bou-Torrent J, Martinez-Garcia JF - The shade avoidance syndrome in Arabidopsis: a fundamental role for atypical basic helix-loop-helix proteins as transcriptional cofactors
Visit the PubMed Articlewe selected At5g57780 as a molecular marker of PAR1 or PAR2 overexpression (At5g57780 expression was reduced in plants with increased PAR1 activity). As shown in Figure 2(a,b), the expression of this gene was down-regulated only in P35S:AHC-G and P35S:HC-G seedlings, as well as in the P35S:PAR1-G control line
Galstyan A, Cifuentes-Esquivel N, Bou-Torrent J, Martinez-Garcia JF - The shade avoidance syndrome in Arabidopsis: a fundamental role for atypical basic helix-loop-helix proteins as transcriptional cofactors
Visit the PubMed Articlewe investigated whether PAR1 could be converted into a transcriptional activator by fusing it to the transactivating domain of the herpes viral protein VP16 (VP16-AD). Despite the non-plant origin of this protein, fusions of VP16-AD to plant DNA-binding transcription factors have been successfully used to convert transcriptional repressors into activators (Steindler et al., 1999; Parcy et al., 2002; Sawa et al., 2002). In the absence of direct or indirect access to DNA regulatory sequences, overexpression of PAR1 fused to VP16-AD (P35S:PAR1-V lines, Figure 3a) was expected to result in a phenotype virtually identical to that observed by overexpressing a wild-type version of PAR1. In agreement, P35S:PAR1-V lines ... simulated shade-induced ... SAUR68 gene expression
Galstyan A, Cifuentes-Esquivel N, Bou-Torrent J, Martinez-Garcia JF - The shade avoidance syndrome in Arabidopsis: a fundamental role for atypical basic helix-loop-helix proteins as transcriptional cofactors
Visit the PubMed Articlethe expression ... was down-regulated ... Overexpression of PAR1-G or truncated AHC-G and HC-G also had very similar effects on the auxin-induced levels of transcripts encoding ... SAUR68
Galstyan A, Cifuentes-Esquivel N, Bou-Torrent J, Martinez-Garcia JF - The shade avoidance syndrome in Arabidopsis: a fundamental role for atypical basic helix-loop-helix proteins as transcriptional cofactors
Visit the PubMed Article35S:AtTZF1 ... expression of the central flowering promoter SOC1 was also reduced in OE1 plants
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed Article35S:AtTZF1 ... down-regulated genes ... cutin biosynthetic acyltransferase (At4g00400
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed Article35S:AtTZF1 ... down-regulated genes ... lipase ... At5g45950
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed Article35S:AtTZF1 ... down-regulated genes ... At1g66760
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed ArticleRNA gel-blot and quantitative RT-PCR analyses were used to confirm the results of the microarray analyses ... GA marker genes AtEXP1 ... down-regulated ... in AtTZF1 OE plants
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed Article35S:AtTZF1 ... enhanced expression of the ABA/cold tolerance marker genes ... RD29A ... under non-acclimatized conditions
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed ArticleNULL
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed ArticleRNA gel-blot and quantitative RT-PCR analyses were used to confirm the results of the microarray analyses ... SEN1 ... down-regulated ... in AtTZF1 OE plants
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed ArticleRNA gel-blot and quantitative RT-PCR analyses were used to confirm the results of the microarray analyses ... GASA6 ... down-regulated ... in AtTZF1 OE plants
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed Article35S:AtTZF1 ... down-regulated genes ... EXPA3
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed ArticleRNA gel-blot and quantitative RT-PCR analyses were used to confirm the results of the microarray analyses ... genes involved in ... drought (ERD11) responses were up-regulated ... in AtTZF1 OE plants
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed ArticleRNA gel-blot and quantitative RT-PCR analyses were used to confirm the results of the microarray analyses ... ASN1 ... down-regulated ... in AtTZF1 OE plants
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed ArticleThe results of our RNA gel-blot analyses showed that both GASA6 ... induced by GA
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed ArticleRNA gel-blot and quantitative RT-PCR analyses were used to confirm the results of the microarray analyses ... AtEXP8 ... down-regulated ... in AtTZF1 OE plants
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed Article35S:AtTZF1 ... Consistent with the role of FT as a florigen ... its level was reduced in OE1 plants
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed ArticleThe results of our RNA gel-blot analyses showed that ... GASA4 ... repressed by ABA at 10 µm concentration
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed ArticleThe results of our RNA gel-blot analyses showed that ... GASA4 ... induced by GA
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed Article35S:AtTZF1 ... down-regulated genes ... lipase ... At4g18970
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed Articlethe expression of GASA4 declined in 6-week-old WT plants. However, GASA4 expression declined to a lesser extent in 6-week-old ... AtTZF1 OE plants
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed Articleglucose repression of AtTZF1 was reduced in HXK loss-of-function plants, including ... HXK1 knockout mutant gin2
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed Articleglucose repression was unaffected in the ABA signaling mutant abi4-1 ... suggesting that ABA is not directly involved in glucose repression of AtTZF1
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed ArticleAtTZF1 was rapidly repressed by as little as 0.1% glucose (Figure 1a,b). As hexokinase (HXK) is important for sugar signaling (Jang et al., 1997; Moore et al., 2003), we used a variety of sugars (Xiao et al., 2000) to determine the role of HXK in sugar repression of AtTZF1. The results showed that the preferred substrates of HXK, including glucose and mannose, triggered strong repression, whereas poor substrates of HXK, such as mannitol and 3-O-methyl glucose (3-OMG) and non-permeable l-glucose, did not
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed ArticleRNA gel-blot and quantitative RT-PCR analyses were used to confirm the results of the microarray analyses ... ABA marker genes ... COR15A were up-regulated ... in AtTZF1 OE plants
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed Article35S:AtTZF1 ... down-regulated genes ... GA-Stimulated Arabidopsis 6 (GASA6, At1g74670
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed Article35S:AtTZF1 ... down-regulated genes ... brassinosteroid-insensitive mutant suppressor 1 (BRS1, At4g30610
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed Article35S:AtTZF1 ... down-regulated genes ... At4g35770
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed ArticleThe results showed that ABA alone did not affect AtTZF1 expression, and glucose repression remained unchanged in the presence of ABA
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed Article35S:AtTZF1 ... down-regulated genes ... ASN1/DIN6 (At3g47340
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed Articlegenes involved in cold (KIN1 ... were up-regulated ... in AtTZF1 OE plants
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed Article35S:AtTZF1 ... down-regulated genes ... EXPA ... 8
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed Articleglucose repression was unaffected in ... the ABA biosynthetic mutant aba2-1, suggesting that ABA is not directly involved in glucose repression of AtTZF1
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed ArticleGA marker genes ... GAI ... down-regulated ... in AtTZF1 OE plants
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed Article35S:AtTZF1 ... down-regulated genes ... EXPA ... 11
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed Article35S:AtTZF1 ... down-regulated genes ... pectate lyase ... At5g48900
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed Article35S:AtTZF1 ... up-regulated genes ... KIN1
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed Articleglucose repression of AtTZF1 was reduced in HXK loss-of-function plants, including ... antisense HXK2
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed Articleglucose repression of AtTZF1 was reduced in HXK loss-of-function plants, including antisense HXK1
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed Article35S:AtTZF1 ... down-regulated genes ... At2g17880
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed Article35S:AtTZF1 ... down-regulated genes ... pectate lyase ... At3g07010
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed ArticleRNA gel-blot and quantitative RT-PCR analyses were used to confirm the results of the microarray analyses ... BRS1 ... down-regulated ... in AtTZF1 OE plants
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed ArticleThe results of our RNA gel-blot analyses showed that both GASA6 ... repressed by ABA at 10 µm concentration
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed Article35S:AtTZF1 ... enhanced expression of the ABA/cold tolerance marker genes ... KIN1 ... under non-acclimatized conditions
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed Article35S:AtTZF1 ... expression of the major flowering repressor FLC ... was higher in OE1 plants
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed ArticleThe expression level of GASA6 decreased with age in WT plants, but it expressed at consistently low levels ... in AtTZF1 OE plants
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed ArticleRNA gel-blot and quantitative RT-PCR analyses were used to confirm the results of the microarray analyses ... ABA marker genes RD29A ... up-regulated ... in AtTZF1 OE plants
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed Article35S:AtTZF1 ... up-regulated genes ... COR15a
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed Article35S:AtTZF1 ... down-regulated genes ... At5g57760
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed Article35S:AtTZF1 ... enhanced expression of the ABA/cold tolerance marker genes COR15A ... under non-acclimatized conditions
Lin PC, Pomeranz MC, Jikumaru Y, Kang SG, Hah C, Fujioka S, Kamiya Y, Jang JC - The Arabidopsis tandem zinc finger protein AtTZF1 affects ABA- and GA-mediated growth, stress and gene expression responses
Visit the PubMed ArticleRPL27aC transcripts were detected uniformly throughout early globular ... stage embryos ... stage
Szakonyi D, Byrne ME - Ribosomal protein L27a is required for growth and patterning in Arabidopsis thaliana
Visit the PubMed ArticleSTM:STM-VENUS was absent or expressed at low levels in enlarged rpl27ac-1d globular-stage embryos
Szakonyi D, Byrne ME - Ribosomal protein L27a is required for growth and patterning in Arabidopsis thaliana
Visit the PubMed ArticleCUC2:CUC2-VENUS was detected in the outer cell layers of rpl27ac-1d embryos
Szakonyi D, Byrne ME - Ribosomal protein L27a is required for growth and patterning in Arabidopsis thaliana
Visit the PubMed ArticleRPL27aC ... Transcripts ... were detected at similar levels in all shoot tissues examined
Szakonyi D, Byrne ME - Ribosomal protein L27a is required for growth and patterning in Arabidopsis thaliana
Visit the PubMed ArticleCUC2:CUC2-VENUS was expressed in the shoot meristem region of transition- and heart-stage wild-type embryos
Szakonyi D, Byrne ME - Ribosomal protein L27a is required for growth and patterning in Arabidopsis thaliana
Visit the PubMed ArticleIn rpl27ac-1d mutants, FIL:dsRED ... was weakly expressed in enlarged globular embryos
Szakonyi D, Byrne ME - Ribosomal protein L27a is required for growth and patterning in Arabidopsis thaliana
Visit the PubMed ArticleIn rpl27ac-1d ... PHB ... expressed in the apical and central regions of enlarged globular embryos, in a pattern similar to wild-type
Szakonyi D, Byrne ME - Ribosomal protein L27a is required for growth and patterning in Arabidopsis thaliana
Visit the PubMed ArticleIn rpl27ac-1d mutants, FIL:dsRED was not detected ... in enlarged globular embryos
Szakonyi D, Byrne ME - Ribosomal protein L27a is required for growth and patterning in Arabidopsis thaliana
Visit the PubMed ArticleIn wild-type, STM:STM-VENUS expression was detected in the shoot meristem of transition- and heart-stage embryos
Szakonyi D, Byrne ME - Ribosomal protein L27a is required for growth and patterning in Arabidopsis thaliana
Visit the PubMed ArticleRPL27aB ... Transcripts ... were detected at similar levels in all shoot tissues examined
Szakonyi D, Byrne ME - Ribosomal protein L27a is required for growth and patterning in Arabidopsis thaliana
Visit the PubMed ArticlePHB ... expressed in the apical and central regions of wild-type embryos
Szakonyi D, Byrne ME - Ribosomal protein L27a is required for growth and patterning in Arabidopsis thaliana
Visit the PubMed ArticleSTM:STM-VENUS was ... clearly detected in the shoot meristem of later-stage rpl27ac-1d embryos
Szakonyi D, Byrne ME - Ribosomal protein L27a is required for growth and patterning in Arabidopsis thaliana
Visit the PubMed ArticlePDF1 transcripts were detected specifically in the outer L1 cell layer of wild-type embryos
Szakonyi D, Byrne ME - Ribosomal protein L27a is required for growth and patterning in Arabidopsis thaliana
Visit the PubMed ArticleREV:REV-VENUS ... expressed in the apical and central regions of wild-type embryos
Szakonyi D, Byrne ME - Ribosomal protein L27a is required for growth and patterning in Arabidopsis thaliana
Visit the PubMed ArticleThe level of RPL27aC transcripts was also reduced in rpl27ac-3 compared to wild-type
Szakonyi D, Byrne ME - Ribosomal protein L27a is required for growth and patterning in Arabidopsis thaliana
Visit the PubMed ArticleIn rpl27ac-1d, CUC2:CUC2-VENUS was detected in the apical region of enlarged globular-stage embryos
Szakonyi D, Byrne ME - Ribosomal protein L27a is required for growth and patterning in Arabidopsis thaliana
Visit the PubMed ArticlePDF1 expression was also confined to the outer cell layer in rpl27ac-1d embryos
Szakonyi D, Byrne ME - Ribosomal protein L27a is required for growth and patterning in Arabidopsis thaliana
Visit the PubMed Articleectopic expression of STM:STM-VENUS was also detected in outer cell layers of rpl27ac-1d embryos
Szakonyi D, Byrne ME - Ribosomal protein L27a is required for growth and patterning in Arabidopsis thaliana
Visit the PubMed ArticleIn rpl27ac-1d, REV:REV-VENUS ... expressed in the apical and central regions of enlarged globular embryos, in a pattern similar to wild-type
Szakonyi D, Byrne ME - Ribosomal protein L27a is required for growth and patterning in Arabidopsis thaliana
Visit the PubMed ArticleRPL27aC transcripts were detected uniformly throughout ... heart-stage embryos
Szakonyi D, Byrne ME - Ribosomal protein L27a is required for growth and patterning in Arabidopsis thaliana
Visit the PubMed ArticleRPL27aC transcripts were detected in rpl27ac-2, but their abundance was significantly reduced relative to wild-type
Szakonyi D, Byrne ME - Ribosomal protein L27a is required for growth and patterning in Arabidopsis thaliana
Visit the PubMed ArticleSPA2–HA ... accumulated to lower levels under FRc than in darkness ... the effect of light on SPA–HA protein levels is not reflected in the respective SPA–HA transcript levels
Balcerowicz M, Fittinghoff K, Wirthmueller L, Maier A, Fackendahl P, Fiene G, Koncz C, Hoecker U - Light exposure of Arabidopsis seedlings causes rapid de-stabilization as well as selective post-translational inactivation of the repressor of photomorphogenesis SPA2
Visit the PubMed Articlewe generated transgenic lines expressing transcriptional fusions of the ... SPA2 5' regulatory sequences with GUS ... SPA2 promoters conferred ubiquitous expression in all tissues examined
Balcerowicz M, Fittinghoff K, Wirthmueller L, Maier A, Fackendahl P, Fiene G, Koncz C, Hoecker U - Light exposure of Arabidopsis seedlings causes rapid de-stabilization as well as selective post-translational inactivation of the repressor of photomorphogenesis SPA2
Visit the PubMed ArticleSPA2 5' regulatory sequences with GUS ... Cross-sections through leaves show that both promoters conferred high expression in mesophyll cells
Balcerowicz M, Fittinghoff K, Wirthmueller L, Maier A, Fackendahl P, Fiene G, Koncz C, Hoecker U - Light exposure of Arabidopsis seedlings causes rapid de-stabilization as well as selective post-translational inactivation of the repressor of photomorphogenesis SPA2
Visit the PubMed Articlewe generated specific antibodies against SPA1 and SPA2. SPA1 protein levels were slightly reduced by FRc exposure (Figure 8a,b), despite the observed FRc-induced increase in SPA1 transcript levels
Balcerowicz M, Fittinghoff K, Wirthmueller L, Maier A, Fackendahl P, Fiene G, Koncz C, Hoecker U - Light exposure of Arabidopsis seedlings causes rapid de-stabilization as well as selective post-translational inactivation of the repressor of photomorphogenesis SPA2
Visit the PubMed Articlewe generated transgenic lines expressing transcriptional fusions of the SPA1 ... 5' regulatory sequences with GUS ... In seedlings grown in darkness and under continuous far-red light (FRc), expression was detected in cotyledons, the hypocotyl and the root
Balcerowicz M, Fittinghoff K, Wirthmueller L, Maier A, Fackendahl P, Fiene G, Koncz C, Hoecker U - Light exposure of Arabidopsis seedlings causes rapid de-stabilization as well as selective post-translational inactivation of the repressor of photomorphogenesis SPA2
Visit the PubMed ArticleSPA1 ... 5' regulatory sequences with GUS ... In differentiated leaves, GUS activity predominated in vascular bundles
Balcerowicz M, Fittinghoff K, Wirthmueller L, Maier A, Fackendahl P, Fiene G, Koncz C, Hoecker U - Light exposure of Arabidopsis seedlings causes rapid de-stabilization as well as selective post-translational inactivation of the repressor of photomorphogenesis SPA2
Visit the PubMed ArticleSPA1 ... 5' regulatory sequences with GUS ... Cross-sections through leaves show ... lower, but detectable, expression in the epidermis
Balcerowicz M, Fittinghoff K, Wirthmueller L, Maier A, Fackendahl P, Fiene G, Koncz C, Hoecker U - Light exposure of Arabidopsis seedlings causes rapid de-stabilization as well as selective post-translational inactivation of the repressor of photomorphogenesis SPA2
Visit the PubMed ArticleSPA2 5' regulatory sequences with GUS ... Cross-sections through leaves show ... lower, but detectable, expression in the epidermis
Balcerowicz M, Fittinghoff K, Wirthmueller L, Maier A, Fackendahl P, Fiene G, Koncz C, Hoecker U - Light exposure of Arabidopsis seedlings causes rapid de-stabilization as well as selective post-translational inactivation of the repressor of photomorphogenesis SPA2
Visit the PubMed ArticleSPA1 ... 5' regulatory sequences with GUS ... Cross-sections through leaves show that both promoters conferred high expression in mesophyll cells
Balcerowicz M, Fittinghoff K, Wirthmueller L, Maier A, Fackendahl P, Fiene G, Koncz C, Hoecker U - Light exposure of Arabidopsis seedlings causes rapid de-stabilization as well as selective post-translational inactivation of the repressor of photomorphogenesis SPA2
Visit the PubMed ArticleSPA2–HA protein accumulated to lower levels than SPA1–HA when expressed from the same promoter ... When expressed from the SPA1 promoter, SPA2–HA levels in FRc were approximately 3–10-fold lower than those of SPA1–HA. Similarly, when expressed from the SPA2 promoter, SPA2–HA levels in FRc were approximately 4–15-fold lower than those of SPA1–HA
Balcerowicz M, Fittinghoff K, Wirthmueller L, Maier A, Fackendahl P, Fiene G, Koncz C, Hoecker U - Light exposure of Arabidopsis seedlings causes rapid de-stabilization as well as selective post-translational inactivation of the repressor of photomorphogenesis SPA2
Visit the PubMed Articlewe generated transgenic lines expressing transcriptional fusions of the SPA1 ... Young leaves also accumulated GUS activity ubiquitously
Balcerowicz M, Fittinghoff K, Wirthmueller L, Maier A, Fackendahl P, Fiene G, Koncz C, Hoecker U - Light exposure of Arabidopsis seedlings causes rapid de-stabilization as well as selective post-translational inactivation of the repressor of photomorphogenesis SPA2
Visit the PubMed Articlewe generated transgenic lines expressing transcriptional fusions of the ... SPA2 5' regulatory sequences with GUS ... Young leaves also accumulated GUS activity ubiquitously
Balcerowicz M, Fittinghoff K, Wirthmueller L, Maier A, Fackendahl P, Fiene G, Koncz C, Hoecker U - Light exposure of Arabidopsis seedlings causes rapid de-stabilization as well as selective post-translational inactivation of the repressor of photomorphogenesis SPA2
Visit the PubMed ArticleSPA2 protein levels were strongly reduced by light, but SPA2 transcript levels were not reduced
Balcerowicz M, Fittinghoff K, Wirthmueller L, Maier A, Fackendahl P, Fiene G, Koncz C, Hoecker U - Light exposure of Arabidopsis seedlings causes rapid de-stabilization as well as selective post-translational inactivation of the repressor of photomorphogenesis SPA2
Visit the PubMed Articlewe generated transgenic lines expressing transcriptional fusions of the SPA1 ... 5' regulatory sequences with GUS ... SPA1 ... promoters conferred ubiquitous expression in all tissues examined
Balcerowicz M, Fittinghoff K, Wirthmueller L, Maier A, Fackendahl P, Fiene G, Koncz C, Hoecker U - Light exposure of Arabidopsis seedlings causes rapid de-stabilization as well as selective post-translational inactivation of the repressor of photomorphogenesis SPA2
Visit the PubMed ArticleSPA2 5' regulatory sequences with GUS ... In differentiated leaves, GUS activity predominated in vascular bundles
Balcerowicz M, Fittinghoff K, Wirthmueller L, Maier A, Fackendahl P, Fiene G, Koncz C, Hoecker U - Light exposure of Arabidopsis seedlings causes rapid de-stabilization as well as selective post-translational inactivation of the repressor of photomorphogenesis SPA2
Visit the PubMed ArticleSPA1–HA ... accumulated to lower levels under FRc than in darkness ... the effect of light on SPA–HA protein levels is not reflected in the respective SPA–HA transcript levels
Balcerowicz M, Fittinghoff K, Wirthmueller L, Maier A, Fackendahl P, Fiene G, Koncz C, Hoecker U - Light exposure of Arabidopsis seedlings causes rapid de-stabilization as well as selective post-translational inactivation of the repressor of photomorphogenesis SPA2
Visit the PubMed Articlewe generated transgenic lines expressing transcriptional fusions of the ... SPA2 5' regulatory sequences with GUS ... In seedlings grown in darkness and under continuous far-red light (FRc), expression was detected in cotyledons, the hypocotyl and the root
Balcerowicz M, Fittinghoff K, Wirthmueller L, Maier A, Fackendahl P, Fiene G, Koncz C, Hoecker U - Light exposure of Arabidopsis seedlings causes rapid de-stabilization as well as selective post-translational inactivation of the repressor of photomorphogenesis SPA2
Visit the PubMed ArticleSPA2–HA protein levels were strongly reduced within 1 h of FRc irradiation (Figure 7a,b), indicating that light causes a rapid decrease in SPA–HA protein levels ... SPA–HA transcript levels were not altered by light exposure (Figure 7d), suggesting that light down-regulates SPA–HA protein levels post-transcriptionally by causing SPA protein degradation
Balcerowicz M, Fittinghoff K, Wirthmueller L, Maier A, Fackendahl P, Fiene G, Koncz C, Hoecker U - Light exposure of Arabidopsis seedlings causes rapid de-stabilization as well as selective post-translational inactivation of the repressor of photomorphogenesis SPA2
Visit the PubMed ArticleSPA1–HA ... protein levels were strongly reduced within 1 h of FRc irradiation (Figure 7a,b), indicating that light causes a rapid decrease in SPA–HA protein levels ... SPA–HA transcript levels were not altered by light exposure (Figure 7d), suggesting that light down-regulates SPA–HA protein levels post-transcriptionally by causing SPA protein degradation
Balcerowicz M, Fittinghoff K, Wirthmueller L, Maier A, Fackendahl P, Fiene G, Koncz C, Hoecker U - Light exposure of Arabidopsis seedlings causes rapid de-stabilization as well as selective post-translational inactivation of the repressor of photomorphogenesis SPA2
Visit the PubMed Articlethe reciprocal hybrids from crossing the strongest overexpressors, i.e. AtPDX1.1 OE line 2 and AtPDX2 OE line 4 were chosen for detailed analysis. For clarity, the hybrid resulting from AtPDX1.1 OE L2 as male parent and AtPDX2 OE L4 as female parent is designated line 1 and that from AtPDX1.1 OE L2 as female parent and AtPDX2 OE L4 as male parent is designated line 2 (Figure 2c). The free vitamin B6 content of shoot material from the double overexpressors was increased up to four-fold over wild-type ... it appears that the ... SOS4 transcript levels are ... downregulated
Raschke M, Boycheva S, Crèvecoeur M, Nunes-Nesi A, Witt S, Fernie AR, Amrhein N, Fitzpatrick TB - Enhanced levels of vitamin B(6) increase aerial organ size and positively affect stress tolerance in Arabidopsis
Visit the PubMed Articlethe reciprocal hybrids from crossing the strongest overexpressors, i.e. AtPDX1.1 OE line 2 and AtPDX2 OE line 4 were chosen for detailed analysis. For clarity, the hybrid resulting from AtPDX1.1 OE L2 as male parent and AtPDX2 OE L4 as female parent is designated line 1 and that from AtPDX1.1 OE L2 as female parent and AtPDX2 OE L4 as male parent is designated line 2 (Figure 2c). The free vitamin B6 content of shoot material from the double overexpressors was increased up to four-fold over wild-type ... it appears that the PDX3 ... transcript levels are upregulated
Raschke M, Boycheva S, Crèvecoeur M, Nunes-Nesi A, Witt S, Fernie AR, Amrhein N, Fitzpatrick TB - Enhanced levels of vitamin B(6) increase aerial organ size and positively affect stress tolerance in Arabidopsis
Visit the PubMed ArticleWe found a slight increase in transcript levels of ... YAB5 ... in the leaves of the as2-1 mutant
Horiguchi G, Mollá-Morales A, Pérez-Pérez JM, Kojima K, Robles P, Ponce MR, Micol JL, Tsukaya H - Differential contributions of ribosomal protein genes to Arabidopsis thaliana leaf development
Visit the PubMed Articleusing semi-quantitative RT-PCR ... RPL4D mRNA was undetectable in the T-DNA insertional mutant rpl4d-4
Horiguchi G, Mollá-Morales A, Pérez-Pérez JM, Kojima K, Robles P, Ponce MR, Micol JL, Tsukaya H - Differential contributions of ribosomal protein genes to Arabidopsis thaliana leaf development
Visit the PubMed ArticlemRNA ... levels of its homologue, RPL4A, were similar in ... rpl4d-4 and the wild-type
Horiguchi G, Mollá-Morales A, Pérez-Pérez JM, Kojima K, Robles P, Ponce MR, Micol JL, Tsukaya H - Differential contributions of ribosomal protein genes to Arabidopsis thaliana leaf development
Visit the PubMed ArticlemRNA ... levels of its homologue, RPL4A, were similar in rpl4d-3 ... and the wild-type
Horiguchi G, Mollá-Morales A, Pérez-Pérez JM, Kojima K, Robles P, Ponce MR, Micol JL, Tsukaya H - Differential contributions of ribosomal protein genes to Arabidopsis thaliana leaf development
Visit the PubMed ArticleWe found a slight increase in transcript levels of ... ARF3 ... in the leaves of the as2-1 mutant
Horiguchi G, Mollá-Morales A, Pérez-Pérez JM, Kojima K, Robles P, Ponce MR, Micol JL, Tsukaya H - Differential contributions of ribosomal protein genes to Arabidopsis thaliana leaf development
Visit the PubMed Articleagreement with the synergistic polarity defects observed in rpl4d-3 as2-1 ... expression ... REV were expressed at similar levels in the ... parents
Horiguchi G, Mollá-Morales A, Pérez-Pérez JM, Kojima K, Robles P, Ponce MR, Micol JL, Tsukaya H - Differential contributions of ribosomal protein genes to Arabidopsis thaliana leaf development
Visit the PubMed Articleusing semi-quantitative RT-PCR ... RPL4D mRNA ... was reduced in rpl4d-3
Horiguchi G, Mollá-Morales A, Pérez-Pérez JM, Kojima K, Robles P, Ponce MR, Micol JL, Tsukaya H - Differential contributions of ribosomal protein genes to Arabidopsis thaliana leaf development
Visit the PubMed ArticleRPS6A transcripts were ... similar in rps6a-1 and the wild-type
Horiguchi G, Mollá-Morales A, Pérez-Pérez JM, Kojima K, Robles P, Ponce MR, Micol JL, Tsukaya H - Differential contributions of ribosomal protein genes to Arabidopsis thaliana leaf development
Visit the PubMed Articlewe performed a quantitative real-time PCR analysis. We found a significant reduction in RPL7B expression levels in den5 compared to the wild-type
Horiguchi G, Mollá-Morales A, Pérez-Pérez JM, Kojima K, Robles P, Ponce MR, Micol JL, Tsukaya H - Differential contributions of ribosomal protein genes to Arabidopsis thaliana leaf development
Visit the PubMed ArticleRPS6A transcripts were not detected in the rps6a-2 mutant, which carries a reorganization in RPS6A 3′ UTR
Horiguchi G, Mollá-Morales A, Pérez-Pérez JM, Kojima K, Robles P, Ponce MR, Micol JL, Tsukaya H - Differential contributions of ribosomal protein genes to Arabidopsis thaliana leaf development
Visit the PubMed ArticleIn agreement with the synergistic polarity defects observed in rpl4d-3 as2-1, we found a strong increase in ... KAN2 ... expression
Horiguchi G, Mollá-Morales A, Pérez-Pérez JM, Kojima K, Robles P, Ponce MR, Micol JL, Tsukaya H - Differential contributions of ribosomal protein genes to Arabidopsis thaliana leaf development
Visit the PubMed ArticleIn agreement with the synergistic polarity defects observed in rpl4d-3 as2-1 ... expression ... PHV ... were expressed at similar levels in ... its parents
Horiguchi G, Mollá-Morales A, Pérez-Pérez JM, Kojima K, Robles P, Ponce MR, Micol JL, Tsukaya H - Differential contributions of ribosomal protein genes to Arabidopsis thaliana leaf development
Visit the PubMed ArticleWe found a slight increase in transcript levels ... of ... KAN2 ... in the leaves of the as2-1 mutant
Horiguchi G, Mollá-Morales A, Pérez-Pérez JM, Kojima K, Robles P, Ponce MR, Micol JL, Tsukaya H - Differential contributions of ribosomal protein genes to Arabidopsis thaliana leaf development
Visit the PubMed Articletranscripts ... levels of its paralogue, RPL6B, were comparable in ... the three rps6a mutants and their wild-type
Horiguchi G, Mollá-Morales A, Pérez-Pérez JM, Kojima K, Robles P, Ponce MR, Micol JL, Tsukaya H - Differential contributions of ribosomal protein genes to Arabidopsis thaliana leaf development
Visit the PubMed ArticleIn agreement with the synergistic polarity defects observed in rpl4d-3 as2-1, we found a strong increase in ARF3 ... expression
Horiguchi G, Mollá-Morales A, Pérez-Pérez JM, Kojima K, Robles P, Ponce MR, Micol JL, Tsukaya H - Differential contributions of ribosomal protein genes to Arabidopsis thaliana leaf development
Visit the PubMed ArticleIn agreement with the synergistic polarity defects observed in rpl4d-3 as2-1, we found a strong increase in ... YAB5 expression
Horiguchi G, Mollá-Morales A, Pérez-Pérez JM, Kojima K, Robles P, Ponce MR, Micol JL, Tsukaya H - Differential contributions of ribosomal protein genes to Arabidopsis thaliana leaf development
Visit the PubMed Articlerps6a-3 ... a significant reduction in RPS6A mRNA
Horiguchi G, Mollá-Morales A, Pérez-Pérez JM, Kojima K, Robles P, Ponce MR, Micol JL, Tsukaya H - Differential contributions of ribosomal protein genes to Arabidopsis thaliana leaf development
Visit the PubMed ArticleIn agreement with the synergistic polarity defects observed in rpl4d-3 as2 ... - ... 1 ... expression ... PHB ... were expressed at similar levels ... in ... its parents
Horiguchi G, Mollá-Morales A, Pérez-Pérez JM, Kojima K, Robles P, Ponce MR, Micol JL, Tsukaya H - Differential contributions of ribosomal protein genes to Arabidopsis thaliana leaf development
Visit the PubMed ArticleRPS6A transcripts were ... reduced in rps6a-3, which carries a mutation outside the RPS6A transcription unit
Horiguchi G, Mollá-Morales A, Pérez-Pérez JM, Kojima K, Robles P, Ponce MR, Micol JL, Tsukaya H - Differential contributions of ribosomal protein genes to Arabidopsis thaliana leaf development
Visit the PubMed ArticleBRCA2a ... significantly up-regulated in Atasf1ab
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed ArticleIn Atasf1ab, only ... RAD54 ... induced by HU, and the other examined genes (excepting those of the NHEJ pathway) remained at relatively similar high levels in HU-treated and HU-untreated plants
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed ArticleOur quantitative RT-PCR analysis revealed that expression of H3.1 and CYCB1;1, but not CYCD3;3 ... is increased ... in Atasf1ab compared to WT
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed ArticleAtASF1A ... ubiquitously expressed in most of the Arabidopsis plant tissues
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed Articlein Atasf1ab ... CDT1A ... up-regulated
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed ArticleIn Atasf1ab, only RAD51 ... induced by HU, and the other examined genes (excepting those of the NHEJ pathway) remained at relatively similar high levels in HU-treated and HU-untreated plants
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed ArticlePARP2 ... also up-regulated in Atasf1ab
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed Articlein Atasf1ab ... MCM4 ... up-regulated
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed Articlein Atasf1ab ... CDC6A ... up-regulated
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed ArticleRAD17 ... significantly up-regulated in Atasf1ab
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed ArticleWe examined by confocal fluorescence microscopy the subcellular localization of EYFP-fused AtASF1A and AtASF1B proteins in the transgenic tobacco BY2 cells. All the fusion proteins were ... absent from the nucleolus ... EYFP-AtASF1B
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed ArticleXRCC3 ... significantly up-regulated in Atasf1ab
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed Articlein Atasf1ab ... PCNA1 ... up-regulated
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed ArticleOur quantitative RT-PCR analysis revealed that expression of ... CYCB1;1 ... is increased by above 1.5-fold in Atasf1ab compared to WT
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed ArticleWe found that ... ATR ... up-regulated in Atasf1ab
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed Articlein Atasf1ab ... Ku80 ... barely affected
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed Articlein Atasf1ab ... MCM7 ... up-regulated
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed ArticleWe examined by confocal fluorescence microscopy the subcellular localization of EYFP-fused AtASF1A and AtASF1B proteins in the transgenic tobacco BY2 cells. All the fusion proteins were observed in ... the cytoplasm ... EYFP-AtASF1B
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed ArticlePARP1 ... also up-regulated in Atasf1ab
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed Articlein Atasf1ab ... MCM5 ... up-regulated
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed Articlein Atasf1ab ... MCM3 ... up-regulated
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed ArticleWe examined by confocal fluorescence microscopy the subcellular localization of EYFP-fused AtASF1A and AtASF1B proteins in the transgenic tobacco BY2 cells. All the fusion proteins were ... absent from the nucleolus ... EYFP-AtASF1A
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed ArticleRAD51C ... significantly up-regulated in Atasf1ab
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed ArticleAtASF1B ... ubiquitously expressed in most of the Arabidopsis plant tissues
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed ArticleSMC6A ... significantly up-regulated in Atasf1ab
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed Articlein Atasf1ab ... Ku70 ... barely affected
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed ArticleWe examined by confocal fluorescence microscopy the subcellular localization of EYFP-fused AtASF1A and AtASF1B proteins in the transgenic tobacco BY2 cells. All the fusion proteins were observed in ... the nucleus ... EYFP-AtASF1A
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed Articlein Atasf1ab ... XRCC4 ... barely affected
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed ArticleRAD51 ... significantly up-regulated in Atasf1ab
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed ArticleWe examined by confocal fluorescence microscopy the subcellular localization of EYFP-fused AtASF1A and AtASF1B proteins in the transgenic tobacco BY2 cells. All the fusion proteins were observed in ... the cytoplasm ... EYFP-AtASF1A
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed ArticleOur quantitative RT-PCR analysis revealed that expression of H3.1 ... is increased by above 1.5-fold in Atasf1ab compared to WT
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed Articlein Atasf1ab ... CDC6B ... up-regulated
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed Articlein Atasf1ab ... CDT1B ... up-regulated
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed ArticleOur quantitative RT-PCR analysis revealed that expression of H3.1 and CYCB1;1, but not ... CYCA2;1, is increased ... in Atasf1ab compared to WT
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed ArticleWe examined by confocal fluorescence microscopy the subcellular localization of EYFP-fused AtASF1A and AtASF1B proteins in the transgenic tobacco BY2 cells. All the fusion proteins were observed in ... the nucleus ... EYFP-AtASF1B
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed ArticleWe found that ... ATM ... up-regulated in Atasf1ab
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed ArticleRAD54 ... significantly up-regulated in Atasf1ab
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed ArticleSMC6B ... significantly up-regulated in Atasf1ab
Zhu Y, Weng M, Yang Y, Zhang C, Li Z, Shen WH, Dong A - Arabidopsis homologues of the histone chaperone ASF1 are crucial for chromatin replication and cell proliferation in plant development
Visit the PubMed ArticleProCUC3:GUS ... GUS activity is absent from the sinus of older teeth
Hasson A, Plessis A, Blein T, Adroher B, Grigg S, Tsiantis M, Boudaoud A, Damerval C, Laufs P - Evolution and diverse roles of the CUP-SHAPED COTYLEDON genes in Arabidopsis leaf development
Visit the PubMed ArticleIn the mir164a-4 ProCUC2:CUC1 line ... KNAT2 ... promoter activity was observed in foci within the blade that could correspond to the developing ectopic meristems and in the sinus region between outgrowing leaflets
Hasson A, Plessis A, Blein T, Adroher B, Grigg S, Tsiantis M, Boudaoud A, Damerval C, Laufs P - Evolution and diverse roles of the CUP-SHAPED COTYLEDON genes in Arabidopsis leaf development
Visit the PubMed ArticleRT-PCR indicated that CUC3 mRNAs ... were detected in developing leaves
Hasson A, Plessis A, Blein T, Adroher B, Grigg S, Tsiantis M, Boudaoud A, Damerval C, Laufs P - Evolution and diverse roles of the CUP-SHAPED COTYLEDON genes in Arabidopsis leaf development
Visit the PubMed ArticleProCUC3:GUS ... Later, GUS activity marks the sinus of the developing serrations
Hasson A, Plessis A, Blein T, Adroher B, Grigg S, Tsiantis M, Boudaoud A, Damerval C, Laufs P - Evolution and diverse roles of the CUP-SHAPED COTYLEDON genes in Arabidopsis leaf development
Visit the PubMed ArticleIn the mir164a-4 ProCUC2:CUC1 line ... MIR164A ... exhibited ... ectopic expression in regions where ectopic KNOX expression occurred
Hasson A, Plessis A, Blein T, Adroher B, Grigg S, Tsiantis M, Boudaoud A, Damerval C, Laufs P - Evolution and diverse roles of the CUP-SHAPED COTYLEDON genes in Arabidopsis leaf development
Visit the PubMed ArticleIn the mir164a-4 ProCUC2:CUC1 line ... CUC3 ... exhibited stronger ... expression
Hasson A, Plessis A, Blein T, Adroher B, Grigg S, Tsiantis M, Boudaoud A, Damerval C, Laufs P - Evolution and diverse roles of the CUP-SHAPED COTYLEDON genes in Arabidopsis leaf development
Visit the PubMed Articlecuc2-1 mutant lines expressing CUC3 under the control of the CUC2 promoter ... MIR164A ... expression remained faint
Hasson A, Plessis A, Blein T, Adroher B, Grigg S, Tsiantis M, Boudaoud A, Damerval C, Laufs P - Evolution and diverse roles of the CUP-SHAPED COTYLEDON genes in Arabidopsis leaf development
Visit the PubMed Articlein the cuc2-1 background ... Expression of the CUC1 protein under the control of the CUC2 promoter ... activated CUC2 ... promoter expression in the leaf blade margin
Hasson A, Plessis A, Blein T, Adroher B, Grigg S, Tsiantis M, Boudaoud A, Damerval C, Laufs P - Evolution and diverse roles of the CUP-SHAPED COTYLEDON genes in Arabidopsis leaf development
Visit the PubMed ArticleIn the mir164a-4 ProCUC2:CUC1 line, CUC2 ... exhibited stronger ... expression
Hasson A, Plessis A, Blein T, Adroher B, Grigg S, Tsiantis M, Boudaoud A, Damerval C, Laufs P - Evolution and diverse roles of the CUP-SHAPED COTYLEDON genes in Arabidopsis leaf development
Visit the PubMed ArticleProCUC2:GUS ... is absent from the blade of the cuc2-1 mutant and limited to the blade–petiole junction and leaf base
Hasson A, Plessis A, Blein T, Adroher B, Grigg S, Tsiantis M, Boudaoud A, Damerval C, Laufs P - Evolution and diverse roles of the CUP-SHAPED COTYLEDON genes in Arabidopsis leaf development
Visit the PubMed ArticleIn the mir164a-4 ProCUC2:CUC1 line ... CUC3 ... exhibited ... ectopic expression in regions where ectopic KNOX expression occurred
Hasson A, Plessis A, Blein T, Adroher B, Grigg S, Tsiantis M, Boudaoud A, Damerval C, Laufs P - Evolution and diverse roles of the CUP-SHAPED COTYLEDON genes in Arabidopsis leaf development
Visit the PubMed ArticleExpression of the control construct ProCUC2:CUC2 in the cuc2-1 background restored ... proper activities of ... MIR164A ... at the blade margin
Hasson A, Plessis A, Blein T, Adroher B, Grigg S, Tsiantis M, Boudaoud A, Damerval C, Laufs P - Evolution and diverse roles of the CUP-SHAPED COTYLEDON genes in Arabidopsis leaf development
Visit the PubMed ArticleProCUC3:GUS ... is absent from the blade of the cuc2-1 mutant ... and limited ... to the leaf base
Hasson A, Plessis A, Blein T, Adroher B, Grigg S, Tsiantis M, Boudaoud A, Damerval C, Laufs P - Evolution and diverse roles of the CUP-SHAPED COTYLEDON genes in Arabidopsis leaf development
Visit the PubMed ArticleIn the mir164a-4 ProCUC2:CUC1 line ... KNOX reporter activity was limited to the base of the petiole of both the wild type and mir164a-4 mutants
Hasson A, Plessis A, Blein T, Adroher B, Grigg S, Tsiantis M, Boudaoud A, Damerval C, Laufs P - Evolution and diverse roles of the CUP-SHAPED COTYLEDON genes in Arabidopsis leaf development
Visit the PubMed Articlecuc2-1 mutant lines expressing CUC3 under the control of the CUC2 promoter ... CUC3 expression remained faint
Hasson A, Plessis A, Blein T, Adroher B, Grigg S, Tsiantis M, Boudaoud A, Damerval C, Laufs P - Evolution and diverse roles of the CUP-SHAPED COTYLEDON genes in Arabidopsis leaf development
Visit the PubMed Articlein the cuc2-1 background ... Expression of the CUC1 protein under the control of the CUC2 promoter ... activated ... CUC3 ... promoter expression in the leaf blade margin
Hasson A, Plessis A, Blein T, Adroher B, Grigg S, Tsiantis M, Boudaoud A, Damerval C, Laufs P - Evolution and diverse roles of the CUP-SHAPED COTYLEDON genes in Arabidopsis leaf development
Visit the PubMed ArticleIn the mir164a-4 ProCUC2:CUC1 line, CUC2 ... exhibited ... ectopic expression in regions where ectopic KNOX expression occurred
Hasson A, Plessis A, Blein T, Adroher B, Grigg S, Tsiantis M, Boudaoud A, Damerval C, Laufs P - Evolution and diverse roles of the CUP-SHAPED COTYLEDON genes in Arabidopsis leaf development
Visit the PubMed Articlein the cuc2-1 background ... Expression of the CUC1 protein under the control of the CUC2 promoter ... activated ... MIR164A promoter expression in the leaf blade margin
Hasson A, Plessis A, Blein T, Adroher B, Grigg S, Tsiantis M, Boudaoud A, Damerval C, Laufs P - Evolution and diverse roles of the CUP-SHAPED COTYLEDON genes in Arabidopsis leaf development
Visit the PubMed ArticleProCUC3:GUS ... was detected at the base of the detached leaves
Hasson A, Plessis A, Blein T, Adroher B, Grigg S, Tsiantis M, Boudaoud A, Damerval C, Laufs P - Evolution and diverse roles of the CUP-SHAPED COTYLEDON genes in Arabidopsis leaf development
Visit the PubMed ArticleIn the mir164a-4 ProCUC2:CUC1 line ... STM promoter activity was observed in foci within the blade that could correspond to the developing ectopic meristems and in the sinus region between outgrowing leaflets
Hasson A, Plessis A, Blein T, Adroher B, Grigg S, Tsiantis M, Boudaoud A, Damerval C, Laufs P - Evolution and diverse roles of the CUP-SHAPED COTYLEDON genes in Arabidopsis leaf development
Visit the PubMed ArticleProMIR164A:GUS ... is absent from the blade of the cuc2-1 mutant and limited to ... the leaf tip
Hasson A, Plessis A, Blein T, Adroher B, Grigg S, Tsiantis M, Boudaoud A, Damerval C, Laufs P - Evolution and diverse roles of the CUP-SHAPED COTYLEDON genes in Arabidopsis leaf development
Visit the PubMed ArticleExpression of the control construct ProCUC2:CUC2 in the cuc2-1 background restored ... proper activities of ... CUC2 ... at the blade margin
Hasson A, Plessis A, Blein T, Adroher B, Grigg S, Tsiantis M, Boudaoud A, Damerval C, Laufs P - Evolution and diverse roles of the CUP-SHAPED COTYLEDON genes in Arabidopsis leaf development
Visit the PubMed ArticleExpression of the control construct ProCUC2:CUC2 in the cuc2-1 background restored ... proper activities of ... CUC3 ... at the blade margin
Hasson A, Plessis A, Blein T, Adroher B, Grigg S, Tsiantis M, Boudaoud A, Damerval C, Laufs P - Evolution and diverse roles of the CUP-SHAPED COTYLEDON genes in Arabidopsis leaf development
Visit the PubMed ArticleIn the mir164a-4 ProCUC2:CUC1 line ... KNAT1 ... promoter activity was observed in foci within the blade that could correspond to the developing ectopic meristems and in the sinus region between outgrowing leaflets
Hasson A, Plessis A, Blein T, Adroher B, Grigg S, Tsiantis M, Boudaoud A, Damerval C, Laufs P - Evolution and diverse roles of the CUP-SHAPED COTYLEDON genes in Arabidopsis leaf development
Visit the PubMed ArticleRT-PCR indicated that ... CUC2 mRNAs ... were detected in developing leaves
Hasson A, Plessis A, Blein T, Adroher B, Grigg S, Tsiantis M, Boudaoud A, Damerval C, Laufs P - Evolution and diverse roles of the CUP-SHAPED COTYLEDON genes in Arabidopsis leaf development
Visit the PubMed ArticleIn the mir164a-4 ProCUC2:CUC1 line ... MIR164A ... exhibited stronger ... expression
Hasson A, Plessis A, Blein T, Adroher B, Grigg S, Tsiantis M, Boudaoud A, Damerval C, Laufs P - Evolution and diverse roles of the CUP-SHAPED COTYLEDON genes in Arabidopsis leaf development
Visit the PubMed Articleno CUC1 mRNA could be detected in developing leaves
Hasson A, Plessis A, Blein T, Adroher B, Grigg S, Tsiantis M, Boudaoud A, Damerval C, Laufs P - Evolution and diverse roles of the CUP-SHAPED COTYLEDON genes in Arabidopsis leaf development
Visit the PubMed Articlein atnuf-1 and atnuf-2 ... Remarkably, accumulation of snoR80, an H/ACA snoRNA derived from the same cluster encoding C/D snoR37, snoR22 and snoR23 showed a 1.7–2.2-fold increase
Rodor J, Jobet E, Bizarro J, Vignols F, Carles C, Suzuki T, Nakamura K, Echeverría M - AtNUFIP, an essential protein for plant development, reveals the impact of snoRNA gene organisation on the assembly of snoRNPs and rRNA methylation in Arabidopsis thaliana
Visit the PubMed ArticleWe first tested the accumulation of U14 and other C/D snoRNAs derived from independent polycistronic clusters. The U14 probe should detect the four U14 isoforms of the cluster which are nearly identical. A reduction of 70–80% was observed for U14 in atnuf-1 and atnuf-2 compared with wild type plants
Rodor J, Jobet E, Bizarro J, Vignols F, Carles C, Suzuki T, Nakamura K, Echeverría M - AtNUFIP, an essential protein for plant development, reveals the impact of snoRNA gene organisation on the assembly of snoRNPs and rRNA methylation in Arabidopsis thaliana
Visit the PubMed Articlein atnuf-1 and atnuf-2 ... Accumulation of snoR22, snoR37 and snoR23 encoded by two duplicated clusters (Barneche et al., 2001) were also drastically reduced in atnuf-1 and atnuf-2 (Figure 6). A similar reduction in both mutants was observed for dicistronic C/D snoRNAs R16 and U43 (Figure 6) and C/D snoRNAs U34a, R19, U36.3, R68 and R40 from six other polycistronic clusters
Rodor J, Jobet E, Bizarro J, Vignols F, Carles C, Suzuki T, Nakamura K, Echeverría M - AtNUFIP, an essential protein for plant development, reveals the impact of snoRNA gene organisation on the assembly of snoRNPs and rRNA methylation in Arabidopsis thaliana
Visit the PubMed ArticleC/D scaR101, predicted to target methylation of U2 (Marker et al., 2002), is encoded by the opposite strand of At1g20690, a predicted gene with no protein-coding capacity. Clearly scaR101 is expressed in wild type plants and is strongly reduced in atnuf-1 and atnuf-2 mutants
Rodor J, Jobet E, Bizarro J, Vignols F, Carles C, Suzuki T, Nakamura K, Echeverría M - AtNUFIP, an essential protein for plant development, reveals the impact of snoRNA gene organisation on the assembly of snoRNPs and rRNA methylation in Arabidopsis thaliana
Visit the PubMed ArticleFinally, we tested the accumulation of C/D snoR43.1, which is processed from a tRNAGly–snoR43 precursor (Kruszka et al., 2003). Northern blots showed a three- to four-fold enhancement of snoR43.1 in both atnuf mutants
Rodor J, Jobet E, Bizarro J, Vignols F, Carles C, Suzuki T, Nakamura K, Echeverría M - AtNUFIP, an essential protein for plant development, reveals the impact of snoRNA gene organisation on the assembly of snoRNPs and rRNA methylation in Arabidopsis thaliana
Visit the PubMed ArticleReduction of C/D scaRNAs should lead to down-methylation of their target snRNAs. We could not test this hypothesis because the assay requires a large amount of RNA, which could not be obtained from atnuf mutants. Nevertheless Northern blot shows that the accumulation of snRNAs is not affected in atnuf mutants (Figure 8c). This finding suggests that the predicted reduction of snRNA methylation in atnuf mutants does not affect snRNA stability
Rodor J, Jobet E, Bizarro J, Vignols F, Carles C, Suzuki T, Nakamura K, Echeverría M - AtNUFIP, an essential protein for plant development, reveals the impact of snoRNA gene organisation on the assembly of snoRNPs and rRNA methylation in Arabidopsis thaliana
Visit the PubMed ArticleFew H/ACA snoRNA polycistronic clusters have been identified in Arabidopsis thaliana. One of these clusters corresponds to the dicistronic cluster encoding H/ACA snoR141 and snoR77 (Chen and Wu, 2009). Northern blot analysis showed that these snoRNAs presented an approximately two-fold increase in atnuf mutants
Rodor J, Jobet E, Bizarro J, Vignols F, Carles C, Suzuki T, Nakamura K, Echeverría M - AtNUFIP, an essential protein for plant development, reveals the impact of snoRNA gene organisation on the assembly of snoRNPs and rRNA methylation in Arabidopsis thaliana
Visit the PubMed ArticleVery few lines expressing GFP–AtNUFIP could be obtained. Moreover, in these lines, the GFP fluorescence was weak and restricted to root cells. GFP–AtNUFIP was localized in the nucleus with a diffused fluorescence in the nucleoplasm and a high concentration in the perinucleolar region (Figure 4b). In meristematic cells from root tips, the fluorescence was concentrated in the external layer of the nucleolus. In elongating root cells, the fluorescence concentrated in perinucleolar foci (Figure 4b). GFP–AtNUFIP was observed neither in the central region of the nucleolus nor in Cajal bodies (Figure 4b). The yellow signal in merged images shows that GFP–AtNUFIP partially co-localized with At15.5K-RFP in the external nucleolar layer and the perinucleolar foci
Rodor J, Jobet E, Bizarro J, Vignols F, Carles C, Suzuki T, Nakamura K, Echeverría M - AtNUFIP, an essential protein for plant development, reveals the impact of snoRNA gene organisation on the assembly of snoRNPs and rRNA methylation in Arabidopsis thaliana
Visit the PubMed ArticleWe also analysed expression of the monocistronic C/D snoRNAs U3 and U13. Accumulation of both snoRNAs increased slightly in atnuf-1 and atnuf-2
Rodor J, Jobet E, Bizarro J, Vignols F, Carles C, Suzuki T, Nakamura K, Echeverría M - AtNUFIP, an essential protein for plant development, reveals the impact of snoRNA gene organisation on the assembly of snoRNPs and rRNA methylation in Arabidopsis thaliana
Visit the PubMed ArticleWe then analysed the accumulation of intronic C/D snoRNAs nested in protein coding genes. The snoR24 (Figure 6) and snoR40 (Figure S4) encoded by two different intronic cluster showed both a 90% reduction in atnuf mutants compared with wild type seedlings
Rodor J, Jobet E, Bizarro J, Vignols F, Carles C, Suzuki T, Nakamura K, Echeverría M - AtNUFIP, an essential protein for plant development, reveals the impact of snoRNA gene organisation on the assembly of snoRNPs and rRNA methylation in Arabidopsis thaliana
Visit the PubMed Articlein atnuf-1 and atnuf-2 mutants ... The 15.5K protein is also a core component of U4 snRNP and it was proposed that NUFIP could be implicated in its assembly (Boulon et al., 2008). We tested the accumulation of U4 in the mutants but no effect was observed
Rodor J, Jobet E, Bizarro J, Vignols F, Carles C, Suzuki T, Nakamura K, Echeverría M - AtNUFIP, an essential protein for plant development, reveals the impact of snoRNA gene organisation on the assembly of snoRNPs and rRNA methylation in Arabidopsis thaliana
Visit the PubMed ArticleC/D scaR102 is predicted to target methylation of U5 (Marker et al., 2002) and was confirmed to be located in plant Cajal bodies (Kim et al., 2010). It was originally described as a monocistronic scaRNA. Nevertheless analysis of flanking genomic sequences reveals a potential C/D scaRX just upstream of scaR102 and a cDNA (AY045928) of 385 nucleotides encompassing both sequences is reported in databanks (Figure 8b). A Northern blot with a scaR102 probe revealed an approximately 160 nucleotide RNA and a longer transcript of approximately 370 nucleotides (Figure 8b). Hybridisation with a probe specific to the scaRX sequence detected only the longer RNA (Figure 8b) revealing that the potential scaRX is not produced in vivo. These data indicate that the 370 nucleotides RNA is a stable transcript that accumulates in vivo and is also a precursor for scaR102 but not for potential scaRX. This finding is reminiscent of some scaRNAs described in animals produced from longer transcripts that also accumulate in vivo (Tycowski et al., 2004). Notably, accumulation of both the scaR102 and the longer transcript was affected in both atnuf-1 and atnuf-2 (Figure 8b). This situation confirms the implication of AtNUFIP in C/D scaR102 assembly and in the stabilisation of the longer transcript
Rodor J, Jobet E, Bizarro J, Vignols F, Carles C, Suzuki T, Nakamura K, Echeverría M - AtNUFIP, an essential protein for plant development, reveals the impact of snoRNA gene organisation on the assembly of snoRNPs and rRNA methylation in Arabidopsis thaliana
Visit the PubMed ArticleTo determine the subcellular localization of AtNUFIP and At15.5K in vivo, we produced transgenic plants co-expressing GFP–AtNUFIP and At15.5K-RFP under the control of the constitutive 35S CaMV promoter. Several transgenic lines were produced expressing At15.5K-RFP in all tissues. The fluorescence was concentrated in the nucleolus, with major labeling in the center of this compartment, and in small foci associated to the nucleolus
Rodor J, Jobet E, Bizarro J, Vignols F, Carles C, Suzuki T, Nakamura K, Echeverría M - AtNUFIP, an essential protein for plant development, reveals the impact of snoRNA gene organisation on the assembly of snoRNPs and rRNA methylation in Arabidopsis thaliana
Visit the PubMed ArticleThese results show that atnuf mutants present a strong reduction of C/D snoRNAs derived from polycistronic precursors which represent the majority of C/D snoRNAs in plants. The C/D snoRNAs derived from monocistronic, single intronic or tRNA-snoRNA precursors as well as the H/ACA snoRNAs show increased accumulation in the mutants
Rodor J, Jobet E, Bizarro J, Vignols F, Carles C, Suzuki T, Nakamura K, Echeverría M - AtNUFIP, an essential protein for plant development, reveals the impact of snoRNA gene organisation on the assembly of snoRNPs and rRNA methylation in Arabidopsis thaliana
Visit the PubMed Articlein atnuf-1 and atnuf-2 ... This finding indicates that the reduction in C/D snoRNA was not due to reduced accumulation or a maturation defect of the polycistronic precursor in the mutants. This fact was confirmed by semi-quantitative RT-PCR that showed no difference in the snoRNA polycistronic precursor level in the mutants compared with wild type plants
Rodor J, Jobet E, Bizarro J, Vignols F, Carles C, Suzuki T, Nakamura K, Echeverría M - AtNUFIP, an essential protein for plant development, reveals the impact of snoRNA gene organisation on the assembly of snoRNPs and rRNA methylation in Arabidopsis thaliana
Visit the PubMed ArticleTo determine the subcellular localization of AtNUFIP and At15.5K in vivo, we produced transgenic plants co-expressing GFP–AtNUFIP and At15.5K-RFP under the control of the constitutive 35S CaMV promoter. Several transgenic lines were produced expressing At15.5K-RFP in all tissues ... These foci correspond to Cajal bodies revealed by co-localization with U2B''-GFP (Figure 4a), a specific marker of this compartment in plants
Rodor J, Jobet E, Bizarro J, Vignols F, Carles C, Suzuki T, Nakamura K, Echeverría M - AtNUFIP, an essential protein for plant development, reveals the impact of snoRNA gene organisation on the assembly of snoRNPs and rRNA methylation in Arabidopsis thaliana
Visit the PubMed ArticleWe checked the expression of the AtNUFIP gene in different plant tissues by semi-quantitative RT-PCR. Primers were designed on the ORF extremities to amplify the two types of AtNUFIP mRNAs. These primers encompass several introns to distinguish PCR amplification from genomic DNA (Figure 2a). As a control we assessed expression of eEF1-a constitutive gene. AtNUFIP mRNA was specifically detected on all tissues (Figure 2c). Thus AtNUFIP is expressed constitutively but at a low level because a high number of PCR cycles was required to detect it as compared with eEF1-a mRNA
Rodor J, Jobet E, Bizarro J, Vignols F, Carles C, Suzuki T, Nakamura K, Echeverría M - AtNUFIP, an essential protein for plant development, reveals the impact of snoRNA gene organisation on the assembly of snoRNPs and rRNA methylation in Arabidopsis thaliana
Visit the PubMed Articlein atnuf mutants ... different situation was observed for the single intronic snoR60 (Barneche et al., 2000) which was not affected
Rodor J, Jobet E, Bizarro J, Vignols F, Carles C, Suzuki T, Nakamura K, Echeverría M - AtNUFIP, an essential protein for plant development, reveals the impact of snoRNA gene organisation on the assembly of snoRNPs and rRNA methylation in Arabidopsis thaliana
Visit the PubMed ArticleQuantitative RT-PCR analysis at 12 and 27°C confirmed our microarray results, showing that HFR1 mRNA levels were significantly elevated in 27 versus 12°C in wild-type seedlings
Foreman J, Johansson H, Hornitschek P, Josse EM, Fankhauser C, Halliday KJ - Light receptor action is critical for maintaining plant biomass at warm ambient temperatures
Visit the PubMed ArticleConsistent with our previous work, B light promotes the accumulation of slower-migrating phosphorylated forms of HFR1 and this occurs at both 15 and 25°C. Interestingly, warm temperatures lead to a stronger accumulation of both forms of HFR1 compared with cooler conditions
Foreman J, Johansson H, Hornitschek P, Josse EM, Fankhauser C, Halliday KJ - Light receptor action is critical for maintaining plant biomass at warm ambient temperatures
Visit the PubMed ArticleAs we observed the presence of additional slower-migrating PIF4-HA isoforms at 25°C when compared with 15°C, we wanted to test whether temperature change could alter PIF4 phosphorylation. Loading of the 15°C samples was increased relative to 25°C to facilitate detection at this temperature (Figure 3d). Phosphatase treatment led to a depletion of the slower-migrating forms at 25°C, but had no impact at 15°C, suggesting that warm temperatures promote the accumulation of phosphorylated forms of PIF4-HA (Figure 3d). We detected this temperature-induced accumulation of slower-migrating forms during the dark and light phases of a diurnal cycle and in etiolated seedlings, indicating that their appearance did not depend on photoreceptor activity (Figures 3d and S4b). These data indicate that temperature, as well as light, may regulate PIF4 phosphorylation
Foreman J, Johansson H, Hornitschek P, Josse EM, Fankhauser C, Halliday KJ - Light receptor action is critical for maintaining plant biomass at warm ambient temperatures
Visit the PubMed ArticleIn 25°C-grown seedlings, HFR1 accumulates more rapidly and to higher levels after transfer to light. Following a peak, detected 1 h post-dawn in our assay, HFR1 levels gradually fall during the course of the photoperiod
Foreman J, Johansson H, Hornitschek P, Josse EM, Fankhauser C, Halliday KJ - Light receptor action is critical for maintaining plant biomass at warm ambient temperatures
Visit the PubMed Articlein 35S::PIF4-HA seedlings, we consistently observed higher levels of PIF4 protein at 25 versus 15°C under B and R photoperiods (Figure 3b,c). When grown in B photoperiods the increase that we observed at 25°C correlated with the presence of additional slower-migrating bands. Here, PIF4 accumulated post-dawn, peaking at 1 h, and then fell gradually during the light period (Figure 3b). This B-light-mediated PIF4 depletion was not observed in R light, although we detected a mild transient dip in PIF4 post-dawn at 25°C (Figure 3c). However, in R light PIF4 was consistently higher at 25°C compared with 15°C, where PIF4 was barely detectable.
Foreman J, Johansson H, Hornitschek P, Josse EM, Fankhauser C, Halliday KJ - Light receptor action is critical for maintaining plant biomass at warm ambient temperatures
Visit the PubMed Articlewe performed Arabidopsis Affymetrix microarray analysis ... HFR1, a bHLH transcription factor ... exhibited temperature-dependent regulation that is influenced by phyB loss
Foreman J, Johansson H, Hornitschek P, Josse EM, Fankhauser C, Halliday KJ - Light receptor action is critical for maintaining plant biomass at warm ambient temperatures
Visit the PubMed ArticleWhen seedlings were grown at 15°C, reducing Pfr at the start of the night led to a moderate rise in PIF4–HA levels during the night and a subsequent fall in PIF4–HA levels 2 h post-dawn (Figure S4c). However, at 25°C, PIF4–HA levels were constitutively elevated and EOD FR treatment had no detectable impact on PIF4–HA levels, although we did detect a modest fall in PIF4–HA levels 2 h post-dawn
Foreman J, Johansson H, Hornitschek P, Josse EM, Fankhauser C, Halliday KJ - Light receptor action is critical for maintaining plant biomass at warm ambient temperatures
Visit the PubMed ArticleAn immunoblot using extracts from seedlings grown in diurnal B light conditions illustrates that at 15°C, HFR1-HA levels rise gradually during the light phase, with a modest peak 8 h post-dawn
Foreman J, Johansson H, Hornitschek P, Josse EM, Fankhauser C, Halliday KJ - Light receptor action is critical for maintaining plant biomass at warm ambient temperatures
Visit the PubMed ArticleIn B light, HFR1 is particularly effective in restraining hypocotyl growth in the warm. We therefore wanted to establish whether levels of HFR1 protein were subject to temperature regulation under B light. Immunoblot analysis of etiolated seedlings expressing 35S::HFR1-HA illustrates that HFR1 abundance is indeed temperature regulated
Foreman J, Johansson H, Hornitschek P, Josse EM, Fankhauser C, Halliday KJ - Light receptor action is critical for maintaining plant biomass at warm ambient temperatures
Visit the PubMed ArticleWe have also shown that in wild-type seedlings subjected to diurnal cycles, HFR1 mRNA levels are again highly temperature regulated (Figure S5). Here HFR1 transcript exhibits a diurnal pattern with a peak that coincides with dawn
Foreman J, Johansson H, Hornitschek P, Josse EM, Fankhauser C, Halliday KJ - Light receptor action is critical for maintaining plant biomass at warm ambient temperatures
Visit the PubMed ArticleQuantitative RT-PCR analysis at 12 and 27°C confirmed our microarray results, showing that HFR1 mRNA levels were significantly elevated in 27 versus 12°C in wild-type seedlings. Depletion of phyB led to a rise in HFR1 mRNA levels at both temperatures, but a reduced temperature response (wild type, 28.2-fold; phyB, 4.5-fold
Foreman J, Johansson H, Hornitschek P, Josse EM, Fankhauser C, Halliday KJ - Light receptor action is critical for maintaining plant biomass at warm ambient temperatures
Visit the PubMed ArticleTo investigate the function of NHX5 and NHX6, we generated knockout mutants using available T-DNA insertion lines. For each gene, two separate T-DNA lines were selected for single knockouts and designated as nhx5-1, nhx5-2, nhx6-1, and nhx6-2 (see Methods). Single knockouts were genotyped and backcrossed two times before their subsequent use in crosses to generate the two independent double knockout lines nhx5-1 nhx6-1 and nhx5-2 nhx6-2 (see Supplemental Figure 3 online). Expression of NHX5 and NHX6 in all knockouts was confirmed with quantitative real-time PCR
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTranscripts associated with ABA responses were particularly enriched in nhx5 nhx6, including the upregulation of ABA receptors (RCAR 8, 10, and 12) and a downregulation of other ABA signaling components, such as ABI1, ABI2, HAB1, GPA1, and PLDa, among others (Figure 8C
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTo investigate the function of NHX5 and NHX6, we generated knockout mutants using available T-DNA insertion lines. For each gene, two separate T-DNA lines were selected for single knockouts and designated as nhx5-1, nhx5-2, nhx6-1, and nhx6-2 (see Methods). Single knockouts were genotyped and backcrossed two times before their subsequent use in crosses to generate the two independent double knockout lines nhx5-1 nhx6-1 and nhx5-2 nhx6-2 ... Furthermore, the expression of NHX1, NHX2, NHX3, and NHX4, as quantified by qPCR, did not change significantly in either single or double nhx5 nhx6 knockouts
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleAnother class of transcripts showing significantly altered expression in nhx5 nhx6 included genes known to have roles in vesicular trafficking (Figure 8B). These included members of the RAB and ARF GTPase family (Rab5 members RABF2 and RAB3Ge), SNARE proteins (VTI12 and NPSN12), as well as vacuolar sorting receptors (VPS35, VPS20, and VSR1), among others (Figure 8B
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTo investigate the function of NHX5 and NHX6, we generated knockout mutants using available T-DNA insertion lines. For each gene, two separate T-DNA lines were selected for single knockouts and designated as nhx5-1, nhx5-2, nhx6-1, and nhx6-2 (see Methods). Single knockouts were genotyped and backcrossed two times before their subsequent use in crosses to generate the two independent double knockout lines nhx5-1 nhx6-1 and nhx5-2 nhx6-2 ... Furthermore, the expression of NHX1, NHX2, NHX3, and NHX4, as quantified by qPCR, did not change significantly in either single or double nhx5 nhx6 knockouts
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTo investigate the function of NHX5 and NHX6, we generated knockout mutants using available T-DNA insertion lines. For each gene, two separate T-DNA lines were selected for single knockouts and designated as nhx5-1, nhx5-2, nhx6-1, and nhx6-2 (see Methods). Single knockouts were genotyped and backcrossed two times before their subsequent use in crosses to generate the two independent double knockout lines nhx5-1 nhx6-1 and nhx5-2 nhx6-2 (see Supplemental Figure 3 online). Expression of NHX5 and NHX6 in all knockouts was confirmed with quantitative real-time PCR
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTo investigate the function of NHX5 and NHX6, we generated knockout mutants using available T-DNA insertion lines. For each gene, two separate T-DNA lines were selected for single knockouts and designated as nhx5-1, nhx5-2, nhx6-1, and nhx6-2 (see Methods). Single knockouts were genotyped and backcrossed two times before their subsequent use in crosses to generate the two independent double knockout lines nhx5-1 nhx6-1 and nhx5-2 nhx6-2 (see Supplemental Figure 3 online). Expression of NHX5 and NHX6 in all knockouts was confirmed with quantitative real-time PCR
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTranscripts associated with ABA responses were particularly enriched in nhx5 nhx6, including the upregulation of ABA receptors (RCAR 8, 10, and 12) and a downregulation of other ABA signaling components, such as ABI1, ABI2, HAB1, GPA1, and PLDa, among others (Figure 8C
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTo investigate the function of NHX5 and NHX6, we generated knockout mutants using available T-DNA insertion lines. For each gene, two separate T-DNA lines were selected for single knockouts and designated as nhx5-1, nhx5-2, nhx6-1, and nhx6-2 (see Methods). Single knockouts were genotyped and backcrossed two times before their subsequent use in crosses to generate the two independent double knockout lines nhx5-1 nhx6-1 and nhx5-2 nhx6-2 ... Furthermore, the expression of NHX1, NHX2, NHX3, and NHX4, as quantified by qPCR, did not change significantly in either single or double nhx5 nhx6 knockouts
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTo investigate the function of NHX5 and NHX6, we generated knockout mutants using available T-DNA insertion lines. For each gene, two separate T-DNA lines were selected for single knockouts and designated as nhx5-1, nhx5-2, nhx6-1, and nhx6-2 (see Methods). Single knockouts were genotyped and backcrossed two times before their subsequent use in crosses to generate the two independent double knockout lines nhx5-1 nhx6-1 and nhx5-2 nhx6-2 ... Furthermore, the expression of NHX1, NHX2, NHX3, and NHX4, as quantified by qPCR, did not change significantly in either single or double nhx5 nhx6 knockouts
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleAnother class of transcripts showing significantly altered expression in nhx5 nhx6 included genes known to have roles in vesicular trafficking (Figure 8B). These included members of the RAB and ARF GTPase family (Rab5 members RABF2 and RAB3Ge), SNARE proteins (VTI12 and NPSN12), as well as vacuolar sorting receptors (VPS35, VPS20, and VSR1), among others (Figure 8B
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTranscripts associated with ABA responses were particularly enriched in nhx5 nhx6, including the upregulation of ABA receptors (RCAR 8, 10, and 12) and a downregulation of other ABA signaling components, such as ABI1, ABI2, HAB1, GPA1, and PLDa, among others (Figure 8C
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTo investigate the function of NHX5 and NHX6, we generated knockout mutants using available T-DNA insertion lines. For each gene, two separate T-DNA lines were selected for single knockouts and designated as nhx5-1, nhx5-2, nhx6-1, and nhx6-2 (see Methods). Single knockouts were genotyped and backcrossed two times before their subsequent use in crosses to generate the two independent double knockout lines nhx5-1 nhx6-1 and nhx5-2 nhx6-2 ... Furthermore, the expression of NHX1, NHX2, NHX3, and NHX4, as quantified by qPCR, did not change significantly in either single or double nhx5 nhx6 knockouts
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTo investigate the function of NHX5 and NHX6, we generated knockout mutants using available T-DNA insertion lines. For each gene, two separate T-DNA lines were selected for single knockouts and designated as nhx5-1, nhx5-2, nhx6-1, and nhx6-2 (see Methods). Single knockouts were genotyped and backcrossed two times before their subsequent use in crosses to generate the two independent double knockout lines nhx5-1 nhx6-1 and nhx5-2 nhx6-2 ... Furthermore, the expression of NHX1, NHX2, NHX3, and NHX4, as quantified by qPCR, did not change significantly in either single or double nhx5 nhx6 knockouts
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTo investigate the function of NHX5 and NHX6, we generated knockout mutants using available T-DNA insertion lines. For each gene, two separate T-DNA lines were selected for single knockouts and designated as nhx5-1, nhx5-2, nhx6-1, and nhx6-2 (see Methods). Single knockouts were genotyped and backcrossed two times before their subsequent use in crosses to generate the two independent double knockout lines nhx5-1 nhx6-1 and nhx5-2 nhx6-2 ... Furthermore, the expression of NHX1, NHX2, NHX3, and NHX4, as quantified by qPCR, did not change significantly in either single or double nhx5 nhx6 knockouts
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTo investigate the function of NHX5 and NHX6, we generated knockout mutants using available T-DNA insertion lines. For each gene, two separate T-DNA lines were selected for single knockouts and designated as nhx5-1, nhx5-2, nhx6-1, and nhx6-2 (see Methods). Single knockouts were genotyped and backcrossed two times before their subsequent use in crosses to generate the two independent double knockout lines nhx5-1 nhx6-1 and nhx5-2 nhx6-2 (see Supplemental Figure 3 online). Expression of NHX5 and NHX6 in all knockouts was confirmed with quantitative real-time PCR
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleAnother class of transcripts showing significantly altered expression in nhx5 nhx6 included genes known to have roles in vesicular trafficking (Figure 8B). These included members of the RAB and ARF GTPase family (Rab5 members RABF2 and RAB3Ge), SNARE proteins (VTI12 and NPSN12), as well as vacuolar sorting receptors (VPS35, VPS20, and VSR1), among others (Figure 8B
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTranscripts associated with ABA responses were particularly enriched in nhx5 nhx6, including the upregulation of ABA receptors (RCAR 8, 10, and 12) and a downregulation of other ABA signaling components, such as ABI1, ABI2, HAB1, GPA1, and PLDa, among others (Figure 8C
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTo investigate the function of NHX5 and NHX6, we generated knockout mutants using available T-DNA insertion lines. For each gene, two separate T-DNA lines were selected for single knockouts and designated as nhx5-1, nhx5-2, nhx6-1, and nhx6-2 (see Methods). Single knockouts were genotyped and backcrossed two times before their subsequent use in crosses to generate the two independent double knockout lines nhx5-1 nhx6-1 and nhx5-2 nhx6-2 ... Furthermore, the expression of NHX1, NHX2, NHX3, and NHX4, as quantified by qPCR, did not change significantly in either single or double nhx5 nhx6 knockouts
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTranscripts associated with ABA responses were particularly enriched in nhx5 nhx6, including the upregulation of ABA receptors (RCAR 8, 10, and 12) and a downregulation of other ABA signaling components, such as ABI1, ABI2, HAB1, GPA1, and PLDa, among others (Figure 8C
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTranscripts associated with ABA responses were particularly enriched in nhx5 nhx6, including the upregulation of ABA receptors (RCAR 8, 10, and 12) and a downregulation of other ABA signaling components, such as ABI1, ABI2, HAB1, GPA1, and PLDa, among others (Figure 8C
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTo investigate the function of NHX5 and NHX6, we generated knockout mutants using available T-DNA insertion lines. For each gene, two separate T-DNA lines were selected for single knockouts and designated as nhx5-1, nhx5-2, nhx6-1, and nhx6-2 (see Methods). Single knockouts were genotyped and backcrossed two times before their subsequent use in crosses to generate the two independent double knockout lines nhx5-1 nhx6-1 and nhx5-2 nhx6-2 ... Furthermore, the expression of NHX1, NHX2, NHX3, and NHX4, as quantified by qPCR, did not change significantly in either single or double nhx5 nhx6 knockouts
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTo investigate the function of NHX5 and NHX6, we generated knockout mutants using available T-DNA insertion lines. For each gene, two separate T-DNA lines were selected for single knockouts and designated as nhx5-1, nhx5-2, nhx6-1, and nhx6-2 (see Methods). Single knockouts were genotyped and backcrossed two times before their subsequent use in crosses to generate the two independent double knockout lines nhx5-1 nhx6-1 and nhx5-2 nhx6-2 ... Furthermore, the expression of NHX1, NHX2, NHX3, and NHX4, as quantified by qPCR, did not change significantly in either single or double nhx5 nhx6 knockouts
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTo investigate the function of NHX5 and NHX6, we generated knockout mutants using available T-DNA insertion lines. For each gene, two separate T-DNA lines were selected for single knockouts and designated as nhx5-1, nhx5-2, nhx6-1, and nhx6-2 (see Methods). Single knockouts were genotyped and backcrossed two times before their subsequent use in crosses to generate the two independent double knockout lines nhx5-1 nhx6-1 and nhx5-2 nhx6-2 ... Furthermore, the expression of NHX1, NHX2, NHX3, and NHX4, as quantified by qPCR, did not change significantly in either single or double nhx5 nhx6 knockouts
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTranscripts associated with ABA responses were particularly enriched in nhx5 nhx6, including the upregulation of ABA receptors (RCAR 8, 10, and 12) and a downregulation of other ABA signaling components, such as ABI1, ABI2, HAB1, GPA1, and PLDa, among others (Figure 8C
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTo investigate the function of NHX5 and NHX6, we generated knockout mutants using available T-DNA insertion lines. For each gene, two separate T-DNA lines were selected for single knockouts and designated as nhx5-1, nhx5-2, nhx6-1, and nhx6-2 (see Methods). Single knockouts were genotyped and backcrossed two times before their subsequent use in crosses to generate the two independent double knockout lines nhx5-1 nhx6-1 and nhx5-2 nhx6-2 ... Furthermore, the expression of NHX1, NHX2, NHX3, and NHX4, as quantified by qPCR, did not change significantly in either single or double nhx5 nhx6 knockouts
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTo investigate the function of NHX5 and NHX6, we generated knockout mutants using available T-DNA insertion lines. For each gene, two separate T-DNA lines were selected for single knockouts and designated as nhx5-1, nhx5-2, nhx6-1, and nhx6-2 (see Methods). Single knockouts were genotyped and backcrossed two times before their subsequent use in crosses to generate the two independent double knockout lines nhx5-1 nhx6-1 and nhx5-2 nhx6-2 ... Furthermore, the expression of NHX1, NHX2, NHX3, and NHX4, as quantified by qPCR, did not change significantly in either single or double nhx5 nhx6 knockouts
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed Articlenhx5 nhx6 ... Genes belonging to the GO terms “structural constituent of the ribosome,” “translation,” and “ribosome biogenesis” were also downregulated
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleBoth NHX5 and NHX6 were expressed in flowers, flower buds, stems, rosette leaves, and roots
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTo investigate the function of NHX5 and NHX6, we generated knockout mutants using available T-DNA insertion lines. For each gene, two separate T-DNA lines were selected for single knockouts and designated as nhx5-1, nhx5-2, nhx6-1, and nhx6-2 (see Methods). Single knockouts were genotyped and backcrossed two times before their subsequent use in crosses to generate the two independent double knockout lines nhx5-1 nhx6-1 and nhx5-2 nhx6-2 (see Supplemental Figure 3 online). Expression of NHX5 and NHX6 in all knockouts was confirmed with quantitative real-time PCR
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTo investigate the function of NHX5 and NHX6, we generated knockout mutants using available T-DNA insertion lines. For each gene, two separate T-DNA lines were selected for single knockouts and designated as nhx5-1, nhx5-2, nhx6-1, and nhx6-2 (see Methods). Single knockouts were genotyped and backcrossed two times before their subsequent use in crosses to generate the two independent double knockout lines nhx5-1 nhx6-1 and nhx5-2 nhx6-2 (see Supplemental Figure 3 online). Expression of NHX5 and NHX6 in all knockouts was confirmed with quantitative real-time PCR
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTo investigate the function of NHX5 and NHX6, we generated knockout mutants using available T-DNA insertion lines. For each gene, two separate T-DNA lines were selected for single knockouts and designated as nhx5-1, nhx5-2, nhx6-1, and nhx6-2 (see Methods). Single knockouts were genotyped and backcrossed two times before their subsequent use in crosses to generate the two independent double knockout lines nhx5-1 nhx6-1 and nhx5-2 nhx6-2 (see Supplemental Figure 3 online). Expression of NHX5 and NHX6 in all knockouts was confirmed with quantitative real-time PCR
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTo investigate the function of NHX5 and NHX6, we generated knockout mutants using available T-DNA insertion lines. For each gene, two separate T-DNA lines were selected for single knockouts and designated as nhx5-1, nhx5-2, nhx6-1, and nhx6-2 (see Methods). Single knockouts were genotyped and backcrossed two times before their subsequent use in crosses to generate the two independent double knockout lines nhx5-1 nhx6-1 and nhx5-2 nhx6-2 ... Furthermore, the expression of NHX1, NHX2, NHX3, and NHX4, as quantified by qPCR, did not change significantly in either single or double nhx5 nhx6 knockouts
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTo investigate the function of NHX5 and NHX6, we generated knockout mutants using available T-DNA insertion lines. For each gene, two separate T-DNA lines were selected for single knockouts and designated as nhx5-1, nhx5-2, nhx6-1, and nhx6-2 (see Methods). Single knockouts were genotyped and backcrossed two times before their subsequent use in crosses to generate the two independent double knockout lines nhx5-1 nhx6-1 and nhx5-2 nhx6-2 (see Supplemental Figure 3 online). Expression of NHX5 and NHX6 in all knockouts was confirmed with quantitative real-time PCR
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTo investigate the function of NHX5 and NHX6, we generated knockout mutants using available T-DNA insertion lines. For each gene, two separate T-DNA lines were selected for single knockouts and designated as nhx5-1, nhx5-2, nhx6-1, and nhx6-2 (see Methods). Single knockouts were genotyped and backcrossed two times before their subsequent use in crosses to generate the two independent double knockout lines nhx5-1 nhx6-1 and nhx5-2 nhx6-2 ... Furthermore, the expression of NHX1, NHX2, NHX3, and NHX4, as quantified by qPCR, did not change significantly in either single or double nhx5 nhx6 knockouts
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTo investigate the function of NHX5 and NHX6, we generated knockout mutants using available T-DNA insertion lines. For each gene, two separate T-DNA lines were selected for single knockouts and designated as nhx5-1, nhx5-2, nhx6-1, and nhx6-2 (see Methods). Single knockouts were genotyped and backcrossed two times before their subsequent use in crosses to generate the two independent double knockout lines nhx5-1 nhx6-1 and nhx5-2 nhx6-2 (see Supplemental Figure 3 online). Expression of NHX5 and NHX6 in all knockouts was confirmed with quantitative real-time PCR
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTo investigate the function of NHX5 and NHX6, we generated knockout mutants using available T-DNA insertion lines. For each gene, two separate T-DNA lines were selected for single knockouts and designated as nhx5-1, nhx5-2, nhx6-1, and nhx6-2 (see Methods). Single knockouts were genotyped and backcrossed two times before their subsequent use in crosses to generate the two independent double knockout lines nhx5-1 nhx6-1 and nhx5-2 nhx6-2 ... Furthermore, the expression of NHX1, NHX2, NHX3, and NHX4, as quantified by qPCR, did not change significantly in either single or double nhx5 nhx6 knockouts
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTo investigate the function of NHX5 and NHX6, we generated knockout mutants using available T-DNA insertion lines. For each gene, two separate T-DNA lines were selected for single knockouts and designated as nhx5-1, nhx5-2, nhx6-1, and nhx6-2 (see Methods). Single knockouts were genotyped and backcrossed two times before their subsequent use in crosses to generate the two independent double knockout lines nhx5-1 nhx6-1 and nhx5-2 nhx6-2 (see Supplemental Figure 3 online). Expression of NHX5 and NHX6 in all knockouts was confirmed with quantitative real-time PCR
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleAnother class of transcripts showing significantly altered expression in nhx5 nhx6 included genes known to have roles in vesicular trafficking (Figure 8B). These included members of the RAB and ARF GTPase family (Rab5 members RABF2 and RAB3Ge), SNARE proteins (VTI12 and NPSN12), as well as vacuolar sorting receptors (VPS35, VPS20, and VSR1), among others (Figure 8B
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTo investigate the function of NHX5 and NHX6, we generated knockout mutants using available T-DNA insertion lines. For each gene, two separate T-DNA lines were selected for single knockouts and designated as nhx5-1, nhx5-2, nhx6-1, and nhx6-2 (see Methods). Single knockouts were genotyped and backcrossed two times before their subsequent use in crosses to generate the two independent double knockout lines nhx5-1 nhx6-1 and nhx5-2 nhx6-2 ... Furthermore, the expression of NHX1, NHX2, NHX3, and NHX4, as quantified by qPCR, did not change significantly in either single or double nhx5 nhx6 knockouts
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTo investigate the function of NHX5 and NHX6, we generated knockout mutants using available T-DNA insertion lines. For each gene, two separate T-DNA lines were selected for single knockouts and designated as nhx5-1, nhx5-2, nhx6-1, and nhx6-2 (see Methods). Single knockouts were genotyped and backcrossed two times before their subsequent use in crosses to generate the two independent double knockout lines nhx5-1 nhx6-1 and nhx5-2 nhx6-2 ... Furthermore, the expression of NHX1, NHX2, NHX3, and NHX4, as quantified by qPCR, did not change significantly in either single or double nhx5 nhx6 knockouts
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleBoth NHX5 and NHX6 were expressed in flowers, flower buds, stems, rosette leaves, and roots
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleInterestingly, many cell wall–related transcripts were also significantly downregulated, especially those associated with structural proteins (arabinogalactans and facsilins), cell wall–modifying enzymes (expansins, xyloglucan endotransglycosylase, and hydrolases), as well as cell wall–degrading enzymes (polygalacturonase
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTo investigate the function of NHX5 and NHX6, we generated knockout mutants using available T-DNA insertion lines. For each gene, two separate T-DNA lines were selected for single knockouts and designated as nhx5-1, nhx5-2, nhx6-1, and nhx6-2 (see Methods). Single knockouts were genotyped and backcrossed two times before their subsequent use in crosses to generate the two independent double knockout lines nhx5-1 nhx6-1 and nhx5-2 nhx6-2 ... Furthermore, the expression of NHX1, NHX2, NHX3, and NHX4, as quantified by qPCR, did not change significantly in either single or double nhx5 nhx6 knockouts
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTo investigate the function of NHX5 and NHX6, we generated knockout mutants using available T-DNA insertion lines. For each gene, two separate T-DNA lines were selected for single knockouts and designated as nhx5-1, nhx5-2, nhx6-1, and nhx6-2 (see Methods). Single knockouts were genotyped and backcrossed two times before their subsequent use in crosses to generate the two independent double knockout lines nhx5-1 nhx6-1 and nhx5-2 nhx6-2 ... Furthermore, the expression of NHX1, NHX2, NHX3, and NHX4, as quantified by qPCR, did not change significantly in either single or double nhx5 nhx6 knockouts
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleAnother class of transcripts showing significantly altered expression in nhx5 nhx6 included genes known to have roles in vesicular trafficking (Figure 8B). These included members of the RAB and ARF GTPase family (Rab5 members RABF2 and RAB3Ge), SNARE proteins (VTI12 and NPSN12), as well as vacuolar sorting receptors (VPS35, VPS20, and VSR1), among others (Figure 8B
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTranscripts associated with ABA responses were particularly enriched in nhx5 nhx6, including the upregulation of ABA receptors (RCAR 8, 10, and 12) and a downregulation of other ABA signaling components, such as ABI1, ABI2, HAB1, GPA1, and PLDa, among others (Figure 8C
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTo investigate the function of NHX5 and NHX6, we generated knockout mutants using available T-DNA insertion lines. For each gene, two separate T-DNA lines were selected for single knockouts and designated as nhx5-1, nhx5-2, nhx6-1, and nhx6-2 (see Methods). Single knockouts were genotyped and backcrossed two times before their subsequent use in crosses to generate the two independent double knockout lines nhx5-1 nhx6-1 and nhx5-2 nhx6-2 ... Furthermore, the expression of NHX1, NHX2, NHX3, and NHX4, as quantified by qPCR, did not change significantly in either single or double nhx5 nhx6 knockouts
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTo investigate the function of NHX5 and NHX6, we generated knockout mutants using available T-DNA insertion lines. For each gene, two separate T-DNA lines were selected for single knockouts and designated as nhx5-1, nhx5-2, nhx6-1, and nhx6-2 (see Methods). Single knockouts were genotyped and backcrossed two times before their subsequent use in crosses to generate the two independent double knockout lines nhx5-1 nhx6-1 and nhx5-2 nhx6-2 (see Supplemental Figure 3 online). Expression of NHX5 and NHX6 in all knockouts was confirmed with quantitative real-time PCR
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTo investigate the function of NHX5 and NHX6, we generated knockout mutants using available T-DNA insertion lines. For each gene, two separate T-DNA lines were selected for single knockouts and designated as nhx5-1, nhx5-2, nhx6-1, and nhx6-2 (see Methods). Single knockouts were genotyped and backcrossed two times before their subsequent use in crosses to generate the two independent double knockout lines nhx5-1 nhx6-1 and nhx5-2 nhx6-2 ... Furthermore, the expression of NHX1, NHX2, NHX3, and NHX4, as quantified by qPCR, did not change significantly in either single or double nhx5 nhx6 knockouts
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTo investigate the function of NHX5 and NHX6, we generated knockout mutants using available T-DNA insertion lines. For each gene, two separate T-DNA lines were selected for single knockouts and designated as nhx5-1, nhx5-2, nhx6-1, and nhx6-2 (see Methods). Single knockouts were genotyped and backcrossed two times before their subsequent use in crosses to generate the two independent double knockout lines nhx5-1 nhx6-1 and nhx5-2 ... nhx6-2 ... Furthermore, the expression of NHX1, NHX2, NHX3, and NHX4, as quantified by qPCR, did not change significantly in either single or double nhx5 nhx6 knockouts
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleAnother class of transcripts showing significantly altered expression in nhx5 nhx6 included genes known to have roles in vesicular trafficking (Figure 8B). These included members of the RAB and ARF GTPase family (Rab5 members RABF2 and RAB3Ge), SNARE proteins (VTI12 and NPSN12), as well as vacuolar sorting receptors (VPS35, VPS20, and VSR1), among others (Figure 8B
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTo investigate the function of NHX5 and NHX6, we generated knockout mutants using available T-DNA insertion lines. For each gene, two separate T-DNA lines were selected for single knockouts and designated as nhx5-1, nhx5-2, nhx6-1, and nhx6-2 (see Methods). Single knockouts were genotyped and backcrossed two times before their subsequent use in crosses to generate the two independent double knockout lines nhx5-1 nhx6-1 and nhx5-2 nhx6-2 ... Furthermore, the expression of NHX1, NHX2, NHX3, and NHX4, as quantified by qPCR, did not change significantly in either single or double nhx5 nhx6 knockouts
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleAnother class of transcripts showing significantly altered expression in nhx5 nhx6 included genes known to have roles in vesicular trafficking (Figure 8B). These included members of the RAB and ARF GTPase family (Rab5 members RABF2 and RAB3Ge), SNARE proteins (VTI12 and NPSN12), as well as vacuolar sorting receptors (VPS35, VPS20, and VSR1), among others (Figure 8B
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed ArticleTo investigate the function of NHX5 and NHX6, we generated knockout mutants using available T-DNA insertion lines. For each gene, two separate T-DNA lines were selected for single knockouts and designated as nhx5-1, nhx5-2, nhx6-1, and nhx6-2 (see Methods ... Single knockouts were genotyped and backcrossed two times before their subsequent use in crosses to generate the two independent double knockout lines nhx5-1 nhx6-1 and nhx5-2 nhx6-2 (see Supplemental Figure 3 online). Expression of NHX5 and NHX6 in all knockouts was confirmed with quantitative real-time PCR
Bassil E, Ohto MA, Esumi T, Tajima H, Zhu Z, Cagnac O, Belmonte M, Peleg Z, Yamaguchi T, Blumwald E - The Arabidopsis intracellular Na+/H+ antiporters NHX5 and NHX6 are endosome associated and necessary for plant growth and development
Visit the PubMed Articlein the VND7-VP16-GR plants in response to DEX treatment ... Downstream genes, including putative direct targets of VND7 ... encode well-known factors of xylem vessel differentiation, such as ... cellulose synthase subunits ... CesA8/IRX1
Yamaguchi M, Mitsuda N, Ohtani M, Ohme-Takagi M, Kato K, Demura T - VASCULAR-RELATED NAC-DOMAIN7 directly regulates the expression of a broad range of genes for xylem vessel formation
Visit the PubMed Articlecontrast, in the presence of DEX, the expression levels of ... IRX10 were unchanged in the VND7-VP16-GR plants
Yamaguchi M, Mitsuda N, Ohtani M, Ohme-Takagi M, Kato K, Demura T - VASCULAR-RELATED NAC-DOMAIN7 directly regulates the expression of a broad range of genes for xylem vessel formation
Visit the PubMed Articlein the VND7-VP16-GR plants in response to DEX treatment ... Downstream genes, including putative direct targets of VND7 ... encode well-known factors of xylem vessel differentiation, such as ... cellulose synthase subunits ... CesA4/IRX5
Yamaguchi M, Mitsuda N, Ohtani M, Ohme-Takagi M, Kato K, Demura T - VASCULAR-RELATED NAC-DOMAIN7 directly regulates the expression of a broad range of genes for xylem vessel formation
Visit the PubMed Articlein the VND7-VP16-GR plants in response to DEX treatment ... Downstream genes, including putative direct targets of VND7 ... encode well-known factors of xylem vessel differentiation, such as ... xylem cysteine proteases ... XCP1
Yamaguchi M, Mitsuda N, Ohtani M, Ohme-Takagi M, Kato K, Demura T - VASCULAR-RELATED NAC-DOMAIN7 directly regulates the expression of a broad range of genes for xylem vessel formation
Visit the PubMed ArticleThe upregulation of ... LBD15/ASL11 ... was also detected in the VND7-VP16-GR plants after DEX treatment
Yamaguchi M, Mitsuda N, Ohtani M, Ohme-Takagi M, Kato K, Demura T - VASCULAR-RELATED NAC-DOMAIN7 directly regulates the expression of a broad range of genes for xylem vessel formation
Visit the PubMed ArticleIn the VND7-VP16-GR plants ... IRX ... 5 ... significantly upregulated by DEX treatment ... qRT-PCR analysis showed that the levels of ... IRX5/CesA4 mRNAs in VND7-VP16-GR plants rose more than threefold in response to DEX treatment, even in the presence of CHX
Yamaguchi M, Mitsuda N, Ohtani M, Ohme-Takagi M, Kato K, Demura T - VASCULAR-RELATED NAC-DOMAIN7 directly regulates the expression of a broad range of genes for xylem vessel formation
Visit the PubMed ArticleIn contrast, in the presence of DEX, the expression levels of IRX3/CesA7 ... were unchanged in the VND7-VP16-GR plants
Yamaguchi M, Mitsuda N, Ohtani M, Ohme-Takagi M, Kato K, Demura T - VASCULAR-RELATED NAC-DOMAIN7 directly regulates the expression of a broad range of genes for xylem vessel formation
Visit the PubMed ArticleIn the VND7-VP16-GR plants ... IRX ... 12 ... significantly upregulated by DEX treatment
Yamaguchi M, Mitsuda N, Ohtani M, Ohme-Takagi M, Kato K, Demura T - VASCULAR-RELATED NAC-DOMAIN7 directly regulates the expression of a broad range of genes for xylem vessel formation
Visit the PubMed Articlein the VND7-VP16-GR plants in response to DEX treatment ... Downstream genes, including putative direct targets of VND7 ... encode well-known factors of xylem vessel differentiation, such as ... xylem cysteine proteases ... XCP2
Yamaguchi M, Mitsuda N, Ohtani M, Ohme-Takagi M, Kato K, Demura T - VASCULAR-RELATED NAC-DOMAIN7 directly regulates the expression of a broad range of genes for xylem vessel formation
Visit the PubMed ArticleIn order to validate our microarray screen for direct targets of VND7, we performed quantitative RT-PCR (qRT-PCR) for several candidate genes (Figure 2). The mRNA levels of ... LBD30/ASL19/JLO ... significantly rose in the VND7-VP16-GR plants in response to DEX treatment in the presence of CHX ... 7.26-fold
Yamaguchi M, Mitsuda N, Ohtani M, Ohme-Takagi M, Kato K, Demura T - VASCULAR-RELATED NAC-DOMAIN7 directly regulates the expression of a broad range of genes for xylem vessel formation
Visit the PubMed Articlein the VND7-VP16-GR plants in response to DEX treatment ... Downstream genes, including putative direct targets of VND7 ... encode well-known factors of xylem vessel differentiation, such as transcriptional factors ... MYB46
Yamaguchi M, Mitsuda N, Ohtani M, Ohme-Takagi M, Kato K, Demura T - VASCULAR-RELATED NAC-DOMAIN7 directly regulates the expression of a broad range of genes for xylem vessel formation
Visit the PubMed ArticleWe subjected 10-day-old seedlings of VND7-VP16-GR plants to pre-treatment with cycloheximide (CHX), a protein synthesis inhibitor to prevent the production of secondary transcription factors that could then influence gene transcription, followed by treatment with or without DEX ... only three genes were selected as downregulated in the VND7-VP16-GR plants in response to DEX treatment (At2g14520
Yamaguchi M, Mitsuda N, Ohtani M, Ohme-Takagi M, Kato K, Demura T - VASCULAR-RELATED NAC-DOMAIN7 directly regulates the expression of a broad range of genes for xylem vessel formation
Visit the PubMed ArticleThe expression levels of MYB46 ... were increased in the VND7-VP16-GR seedling after DEX treatment
Yamaguchi M, Mitsuda N, Ohtani M, Ohme-Takagi M, Kato K, Demura T - VASCULAR-RELATED NAC-DOMAIN7 directly regulates the expression of a broad range of genes for xylem vessel formation
Visit the PubMed ArticleThe expression levels of ... MYB103 were increased in the VND7-VP16-GR seedling after DEX treatment
Yamaguchi M, Mitsuda N, Ohtani M, Ohme-Takagi M, Kato K, Demura T - VASCULAR-RELATED NAC-DOMAIN7 directly regulates the expression of a broad range of genes for xylem vessel formation
Visit the PubMed ArticleChimeric reporter genes encoding YFP fused to the SV40 nuclear localization signal (NLS), or ß-glucuronidase (GUS), under the control of the XCP1 promoter (Figure 3b) showed strong expression in differentiating xylem vessel cells in both roots and leaves
Yamaguchi M, Mitsuda N, Ohtani M, Ohme-Takagi M, Kato K, Demura T - VASCULAR-RELATED NAC-DOMAIN7 directly regulates the expression of a broad range of genes for xylem vessel formation
Visit the PubMed Articlein the VND7-VP16-GR plants in response to DEX treatment ... Downstream genes, including putative direct targets of VND7 ... encode well-known factors of xylem vessel differentiation, such as ... laccase ... LAC4/IRX12
Yamaguchi M, Mitsuda N, Ohtani M, Ohme-Takagi M, Kato K, Demura T - VASCULAR-RELATED NAC-DOMAIN7 directly regulates the expression of a broad range of genes for xylem vessel formation
Visit the PubMed ArticleIn the VND7-VP16-GR plants ... IRX ... 13 ... significantly upregulated by DEX treatment
Yamaguchi M, Mitsuda N, Ohtani M, Ohme-Takagi M, Kato K, Demura T - VASCULAR-RELATED NAC-DOMAIN7 directly regulates the expression of a broad range of genes for xylem vessel formation
Visit the PubMed Articlein the VND7-VP16-GR plants in response to DEX treatment ... Downstream genes, including putative direct targets of VND7 ... encode well-known factors of xylem vessel differentiation, such as transcriptional factors ... LBD30/ASL19/JLO
Yamaguchi M, Mitsuda N, Ohtani M, Ohme-Takagi M, Kato K, Demura T - VASCULAR-RELATED NAC-DOMAIN7 directly regulates the expression of a broad range of genes for xylem vessel formation
Visit the PubMed Articlein the VND7-VP16-GR plants in response to DEX treatment ... Downstream genes, including putative direct targets of VND7 ... encode well-known factors of xylem vessel differentiation, such as ... a serine protease AtSBT1.1 catalyzing the maturation of a phytosulfokine peptide hormone
Yamaguchi M, Mitsuda N, Ohtani M, Ohme-Takagi M, Kato K, Demura T - VASCULAR-RELATED NAC-DOMAIN7 directly regulates the expression of a broad range of genes for xylem vessel formation
Visit the PubMed ArticleThe upregulation of ... LBD30/ASL19/JLO ... was also detected in the VND7-VP16-GR plants after DEX treatment
Yamaguchi M, Mitsuda N, Ohtani M, Ohme-Takagi M, Kato K, Demura T - VASCULAR-RELATED NAC-DOMAIN7 directly regulates the expression of a broad range of genes for xylem vessel formation
Visit the PubMed ArticleIn contrast, in the presence of DEX, the expression levels of ... IRX8/GALACTURONOSYLTRANSFERASE12 (GAUT12 ... were unchanged in the VND7-VP16-GR plants
Yamaguchi M, Mitsuda N, Ohtani M, Ohme-Takagi M, Kato K, Demura T - VASCULAR-RELATED NAC-DOMAIN7 directly regulates the expression of a broad range of genes for xylem vessel formation
Visit the PubMed Articlein the VND7-VP16-GR plants in response to DEX treatment ... Most of the 300 downstream genes of VND7 are transiently induced during in vitro TE transdifferentiation of Arabidopsis suspension cells ... Downstream genes, including putative direct targets of VND7, are apparently enriched in sets 3 and 8, which showed upregulated expression just when the xylem vessel elements were actively forming, and included VND7 itself
Yamaguchi M, Mitsuda N, Ohtani M, Ohme-Takagi M, Kato K, Demura T - VASCULAR-RELATED NAC-DOMAIN7 directly regulates the expression of a broad range of genes for xylem vessel formation
Visit the PubMed ArticleIn order to validate our microarray screen for direct targets of VND7, we performed quantitative RT-PCR (qRT-PCR) for several candidate genes (Figure 2). The mRNA levels of ... XCP1 ... significantly rose in the VND7-VP16-GR plants in response to DEX treatment in the presence of CHX ... 7.65 ... fold
Yamaguchi M, Mitsuda N, Ohtani M, Ohme-Takagi M, Kato K, Demura T - VASCULAR-RELATED NAC-DOMAIN7 directly regulates the expression of a broad range of genes for xylem vessel formation
Visit the PubMed ArticleIn order to validate our microarray screen for direct targets of VND7, we performed quantitative RT-PCR (qRT-PCR) for several candidate genes (Figure 2). The mRNA levels of MYB46 ... significantly rose in the VND7-VP16-GR plants in response to DEX treatment in the presence of CHX (11.33 ... fold
Yamaguchi M, Mitsuda N, Ohtani M, Ohme-Takagi M, Kato K, Demura T - VASCULAR-RELATED NAC-DOMAIN7 directly regulates the expression of a broad range of genes for xylem vessel formation
Visit the PubMed Articlein the VND7-VP16-GR plants ... we checked the expression pattern of XCP1 after the induction of VND7. qRT-PCR revealed that XCP1 expression was elevated, starting 1 hour after treatment with DEX, and continued to increase during culture
Yamaguchi M, Mitsuda N, Ohtani M, Ohme-Takagi M, Kato K, Demura T - VASCULAR-RELATED NAC-DOMAIN7 directly regulates the expression of a broad range of genes for xylem vessel formation
Visit the PubMed ArticleWe subjected 10-day-old seedlings of VND7-VP16-GR plants to pre-treatment with cycloheximide (CHX), a protein synthesis inhibitor to prevent the production of secondary transcription factors that could then influence gene transcription, followed by treatment with or without DEX ... only three genes were selected as downregulated in the VND7-VP16-GR plants in response to DEX treatment ... At5g47340
Yamaguchi M, Mitsuda N, Ohtani M, Ohme-Takagi M, Kato K, Demura T - VASCULAR-RELATED NAC-DOMAIN7 directly regulates the expression of a broad range of genes for xylem vessel formation
Visit the PubMed ArticleWe subjected 10-day-old seedlings of VND7-VP16-GR plants to pre-treatment with cycloheximide (CHX), a protein synthesis inhibitor to prevent the production of secondary transcription factors that could then influence gene transcription, followed by treatment with or without DEX ... only three genes were selected as downregulated in the VND7-VP16-GR plants in response to DEX treatment ... At5g02650
Yamaguchi M, Mitsuda N, Ohtani M, Ohme-Takagi M, Kato K, Demura T - VASCULAR-RELATED NAC-DOMAIN7 directly regulates the expression of a broad range of genes for xylem vessel formation
Visit the PubMed ArticleIn the VND7-VP16-GR plants ... IRX1 ... significantly upregulated by DEX treatment ... qRT-PCR analysis showed that the levels of IRX1/CesA8 ... in VND7-VP16-GR plants rose more than threefold in response to DEX treatment, even in the presence of CHX
Yamaguchi M, Mitsuda N, Ohtani M, Ohme-Takagi M, Kato K, Demura T - VASCULAR-RELATED NAC-DOMAIN7 directly regulates the expression of a broad range of genes for xylem vessel formation
Visit the PubMed ArticleOsPHO1;2 gene under the control of the Arabidopsis PHO1 promoter ... Quantitative RT-PCR analysis revealed that the expression of ... several mir399 primary transcripts, were underexpressed in the shoots of P5 relative to the WT
Rouached H, Stefanovic A, Secco D, Bulak Arpat A, Gout E, Bligny R, Poirier Y. - Uncoupling phosphate deficiency from its major effects on growth and transcriptome via PHO1 expression in Arabidopsis
Visit the PubMed ArticleThe reduction or absence of induction of genes typically associated with Pi deficiency in the shoots of B1 and B3 lines has also been confirmed by quantitative RT-PCR for ... the Pi transporter ... AtPht1;9 ... These results indicated that despite being Pi deficient, the shoots of the B1 and B3 lines show a very limited response at the gene expression level to this deficiency, compared with Pi-deficient WT plants
Rouached H, Stefanovic A, Secco D, Bulak Arpat A, Gout E, Bligny R, Poirier Y. - Uncoupling phosphate deficiency from its major effects on growth and transcriptome via PHO1 expression in Arabidopsis
Visit the PubMed ArticleOsPHO1;2 gene under the control of the Arabidopsis PHO1 promoter ... Quantitative RT-PCR analysis revealed that the expression of At4 ... underexpressed in the shoots of P5 relative to the WT
Rouached H, Stefanovic A, Secco D, Bulak Arpat A, Gout E, Bligny R, Poirier Y. - Uncoupling phosphate deficiency from its major effects on growth and transcriptome via PHO1 expression in Arabidopsis
Visit the PubMed ArticleThe lack of extensive changes in the gene expression profile in Pi-deficient shoots of B1 and B3 plants grown in external media with high (1000 µm) Pi was re-examined in plants grown in media with low (5 µm) external Pi. Clustal analysis revealed that the majority of genes induced in Pi-deficient WT shoot and roots were also induced in pho1-2, B1 and B3 lines grown in low external Pi medium, although the level of the induction in shoots of both B1 and B3 were often reduced compared with Pi-deficient WT and pho1-2 (Figure 5d,e; Tables S3 and S4). For example, whereas At4 (At5g03545 ... induced approximately 20-fold in shoots of WT-Pi plants, the same genes were only induced between three- and sixfold in shoots of B1 and B3 plants grown on low Pi
Rouached H, Stefanovic A, Secco D, Bulak Arpat A, Gout E, Bligny R, Poirier Y. - Uncoupling phosphate deficiency from its major effects on growth and transcriptome via PHO1 expression in Arabidopsis
Visit the PubMed ArticleOsPHO1;2 gene under the control of the Arabidopsis PHO1 promoter ... Quantitative RT-PCR analysis revealed that the expression of ... AtIPS1 ... underexpressed in the shoots of P5 relative to the WT
Rouached H, Stefanovic A, Secco D, Bulak Arpat A, Gout E, Bligny R, Poirier Y. - Uncoupling phosphate deficiency from its major effects on growth and transcriptome via PHO1 expression in Arabidopsis
Visit the PubMed ArticleThe lack of extensive changes in the gene expression profile in Pi-deficient shoots of B1 and B3 plants grown in external media with high (1000 µm) Pi was re-examined in plants grown in media with low (5 µm) external Pi. Clustal analysis revealed that the majority of genes induced in Pi-deficient WT shoot and roots were also induced in pho1-2, B1 and B3 lines grown in low external Pi medium, although the level of the induction in shoots of both B1 and B3 were often reduced compared with Pi-deficient WT and pho1-2 (Figure 5d,e; Tables S3 and S4). For example, whereas ... SPX1 (At5g20150) were induced approximately 20-fold in shoots of WT-Pi plants, the same genes were only induced between three- and sixfold in shoots of B1 and B3 plants grown on low Pi
Rouached H, Stefanovic A, Secco D, Bulak Arpat A, Gout E, Bligny R, Poirier Y. - Uncoupling phosphate deficiency from its major effects on growth and transcriptome via PHO1 expression in Arabidopsis
Visit the PubMed ArticleOsPHO1;2 gene under the control of the Arabidopsis PHO1 promoter ... Quantitative RT-PCR analysis revealed that the expression of ... AtPht1;9 ... underexpressed in the shoots of P5 relative to the WT
Rouached H, Stefanovic A, Secco D, Bulak Arpat A, Gout E, Bligny R, Poirier Y. - Uncoupling phosphate deficiency from its major effects on growth and transcriptome via PHO1 expression in Arabidopsis
Visit the PubMed ArticleThe reduction or absence of induction of genes typically associated with Pi deficiency in the shoots of B1 and B3 lines has also been confirmed by quantitative RT-PCR for ... and primary transcripts encoding mir399 microRNA ... These results indicated that despite being Pi deficient, the shoots of the B1 and B3 lines show a very limited response at the gene expression level to this deficiency, compared with Pi-deficient WT plants
Rouached H, Stefanovic A, Secco D, Bulak Arpat A, Gout E, Bligny R, Poirier Y. - Uncoupling phosphate deficiency from its major effects on growth and transcriptome via PHO1 expression in Arabidopsis
Visit the PubMed ArticleThe reduction or absence of induction of genes typically associated with Pi deficiency in the shoots of B1 and B3 lines has also been confirmed by quantitative RT-PCR for ... IPS1 ... These results indicated that despite being Pi deficient, the shoots of the B1 and B3 lines show a very limited response at the gene expression level to this deficiency, compared with Pi-deficient WT plants
Rouached H, Stefanovic A, Secco D, Bulak Arpat A, Gout E, Bligny R, Poirier Y. - Uncoupling phosphate deficiency from its major effects on growth and transcriptome via PHO1 expression in Arabidopsis
Visit the PubMed ArticleThe reduction or absence of induction of genes typically associated with Pi deficiency in the shoots of B1 and B3 lines has also been confirmed by quantitative RT-PCR for ... the Pi transporter AtPht1;8 ... These results indicated that despite being Pi deficient, the shoots of the B1 and B3 lines show a very limited response at the gene expression level to this deficiency, compared with Pi-deficient WT plants
Rouached H, Stefanovic A, Secco D, Bulak Arpat A, Gout E, Bligny R, Poirier Y. - Uncoupling phosphate deficiency from its major effects on growth and transcriptome via PHO1 expression in Arabidopsis
Visit the PubMed ArticleThe reduction or absence of induction of genes typically associated with Pi deficiency in the shoots of B1 and B3 lines has also been confirmed by quantitative RT-PCR for At4 ... These results indicated that despite being Pi deficient, the shoots of the B1 and B3 lines show a very limited response at the gene expression level to this deficiency, compared with Pi-deficient WT plants
Rouached H, Stefanovic A, Secco D, Bulak Arpat A, Gout E, Bligny R, Poirier Y. - Uncoupling phosphate deficiency from its major effects on growth and transcriptome via PHO1 expression in Arabidopsis
Visit the PubMed ArticleTo corroborate the aforementioned conclusions, publicly available microarray data were analysed using the Genevestigator tool (Zimmermann et al., 2004). This revealed that three of the four Arabidopsis Tic20 genes are expressed throughout the plant’s life-cycle
Kasmati AR, Töpel M, Patel R, Murtaza G, Jarvis P - Molecular and genetic analyses of Tic20 homologues in Arabidopsis thaliana chloroplasts
Visit the PubMed ArticleTo further characterize the chloroplast developmental defect in the tic20-I mutants, levels of several chloroplast proteins were assessed by immunoblotting ... Tic40 ... somewhat depleted in tic20-I mutant plants, the effect is not strong, and so it is possible that compensatory mechanisms (e.g. reduced turnover) are activated that help to maintain their levels and so compensate for the loss of atTic20-I, or that one of the other Tic20 isoforms (e.g. atTic20-IV) plays a more prominent role
Kasmati AR, Töpel M, Patel R, Murtaza G, Jarvis P - Molecular and genetic analyses of Tic20 homologues in Arabidopsis thaliana chloroplasts
Visit the PubMed ArticleTo corroborate the aforementioned conclusions, publicly available microarray data were analysed using the Genevestigator tool (Zimmermann et al., 2004). This revealed that three of the four Arabidopsis Tic20 genes are expressed throughout the plant’s life-cycle
Kasmati AR, Töpel M, Patel R, Murtaza G, Jarvis P - Molecular and genetic analyses of Tic20 homologues in Arabidopsis thaliana chloroplasts
Visit the PubMed ArticleatTic20-II (At2g47840 ... the subcellular localization of the Arabidopsis Tic20 homologues was assessed by analysing yellow fluorescent protein (YFP) fusion proteins ... the yellow-green fluorescence of YFP was observed in a ring-like pattern around the periphery of each chloroplast. This pattern was strongly reminiscent of the distributions seen for atTic110:YFP and atTic40:YFP fusion proteins in a previous study (Bédard et al., 2007), indicating that the Arabidopsis Tic20 homologues are all targeted to the chloroplast envelope
Kasmati AR, Töpel M, Patel R, Murtaza G, Jarvis P - Molecular and genetic analyses of Tic20 homologues in Arabidopsis thaliana chloroplasts
Visit the PubMed ArticleatTic20-V (At5g55710 ... the subcellular localization of the Arabidopsis Tic20 homologues was assessed by analysing yellow fluorescent protein (YFP) fusion proteins ... the yellow-green fluorescence of YFP was observed in a ring-like pattern around the periphery of each chloroplast. This pattern was strongly reminiscent of the distributions seen for atTic110:YFP and atTic40:YFP fusion proteins in a previous study (Bédard et al., 2007), indicating that the Arabidopsis Tic20 homologues are all targeted to the chloroplast envelope
Kasmati AR, Töpel M, Patel R, Murtaza G, Jarvis P - Molecular and genetic analyses of Tic20 homologues in Arabidopsis thaliana chloroplasts
Visit the PubMed ArticleatTic20-V (At5g55710 ... Considering that most outer envelope proteins do not possess a transit peptide (as the four Arabidopsis Tic20 homologues do) (Hofmann and Theg, 2005), and the aforementioned topological and phylogenetic similarities between the Arabidopsis proteins and psTic20, these results strongly support the hypothesis that Arabidopsis Tic20 homologues are localized in the chloroplast inner envelope membrane
Kasmati AR, Töpel M, Patel R, Murtaza G, Jarvis P - Molecular and genetic analyses of Tic20 homologues in Arabidopsis thaliana chloroplasts
Visit the PubMed ArticleatTic20-V (At5g55710 ... Considering that most outer envelope proteins do not possess a transit peptide (as the four Arabidopsis Tic20 homologues do) (Hofmann and Theg, 2005), and the aforementioned topological and phylogenetic similarities between the Arabidopsis proteins and psTic20, these results strongly support the hypothesis that Arabidopsis Tic20 homologues are localized in the chloroplast inner envelope membrane
Kasmati AR, Töpel M, Patel R, Murtaza G, Jarvis P - Molecular and genetic analyses of Tic20 homologues in Arabidopsis thaliana chloroplasts
Visit the PubMed ArticleTo further characterize the chloroplast developmental defect in the tic20-I mutants, levels of several chloroplast proteins were assessed by immunoblotting ... Dramatic effects were observed in relation to components of the photosynthetic apparatus ... FNR ... with amounts reduced to less than 10% of that seen in the wild type
Kasmati AR, Töpel M, Patel R, Murtaza G, Jarvis P - Molecular and genetic analyses of Tic20 homologues in Arabidopsis thaliana chloroplasts
Visit the PubMed ArticleatTic20-IV (At4g03320 ... the subcellular localization of the Arabidopsis Tic20 homologues was assessed by analysing yellow fluorescent protein (YFP) fusion proteins ... the yellow-green fluorescence of YFP was observed in a ring-like pattern around the periphery of each chloroplast. This pattern was strongly reminiscent of the distributions seen for atTic110:YFP and atTic40:YFP fusion proteins in a previous study (Bédard et al., 2007), indicating that the Arabidopsis Tic20 homologues are all targeted to the chloroplast envelope
Kasmati AR, Töpel M, Patel R, Murtaza G, Jarvis P - Molecular and genetic analyses of Tic20 homologues in Arabidopsis thaliana chloroplasts
Visit the PubMed ArticleatTic20-I (At1g04940 ... Considering that most outer envelope proteins do not possess a transit peptide (as the four Arabidopsis Tic20 homologues do) (Hofmann and Theg, 2005), and the aforementioned topological and phylogenetic similarities between the Arabidopsis proteins and psTic20, these results strongly support the hypothesis that Arabidopsis Tic20 homologues are localized in the chloroplast inner envelope membrane
Kasmati AR, Töpel M, Patel R, Murtaza G, Jarvis P - Molecular and genetic analyses of Tic20 homologues in Arabidopsis thaliana chloroplasts
Visit the PubMed ArticleTo further characterize the chloroplast developmental defect in the tic20-I mutants, levels of several chloroplast proteins were assessed by immunoblotting ... Surprisingly, levels of most of the translocon components investigated were not dramatically affected by the tic20 mutations
Kasmati AR, Töpel M, Patel R, Murtaza G, Jarvis P - Molecular and genetic analyses of Tic20 homologues in Arabidopsis thaliana chloroplasts
Visit the PubMed ArticleatTic20-IV (At4g03320 ... Considering that most outer envelope proteins do not possess a transit peptide (as the four Arabidopsis Tic20 homologues do) (Hofmann and Theg, 2005), and the aforementioned topological and phylogenetic similarities between the Arabidopsis proteins and psTic20, these results strongly support the hypothesis that Arabidopsis Tic20 homologues are localized in the chloroplast inner envelope membrane
Kasmati AR, Töpel M, Patel R, Murtaza G, Jarvis P - Molecular and genetic analyses of Tic20 homologues in Arabidopsis thaliana chloroplasts
Visit the PubMed ArticleatTic20-II (At2g47840 ... Considering that most outer envelope proteins do not possess a transit peptide (as the four Arabidopsis Tic20 homologues do) (Hofmann and Theg, 2005), and the aforementioned topological and phylogenetic similarities between the Arabidopsis proteins and psTic20, these results strongly support the hypothesis that Arabidopsis Tic20 homologues are localized in the chloroplast inner envelope membrane
Kasmati AR, Töpel M, Patel R, Murtaza G, Jarvis P - Molecular and genetic analyses of Tic20 homologues in Arabidopsis thaliana chloroplasts
Visit the PubMed ArticleTo further characterize the chloroplast developmental defect in the tic20-I mutants, levels of several chloroplast proteins were assessed by immunoblotting ... Dramatic effects were observed in relation to components of the photosynthetic apparatus ... OE33 ... with amounts reduced to less than 10% of that seen in the wild type
Kasmati AR, Töpel M, Patel R, Murtaza G, Jarvis P - Molecular and genetic analyses of Tic20 homologues in Arabidopsis thaliana chloroplasts
Visit the PubMed ArticleTo further characterize the chloroplast developmental defect in the tic20-I mutants, levels of several chloroplast proteins were assessed by immunoblotting ... Tic110 ... somewhat depleted in tic20-I mutant plants, the effect is not strong, and so it is possible that compensatory mechanisms (e.g. reduced turnover) are activated that help to maintain their levels and so compensate for the loss of atTic20-I, or that one of the other Tic20 isoforms (e.g. atTic20-IV) plays a more prominent role
Kasmati AR, Töpel M, Patel R, Murtaza G, Jarvis P - Molecular and genetic analyses of Tic20 homologues in Arabidopsis thaliana chloroplasts
Visit the PubMed ArticleTo gain insight into the functional relationships between the Arabidopsis Tic20 homologues, their developmental and tissue-specific gene expression patterns were studied by quantitative real-time RT-PCR (Figure 3). The results indicated that atTIC20-I expression is highest in mature photosynthetic tissues (e.g. 14 dL and Rosettes in Figure 3), and relatively weak in non-photosynthetic tissues
Kasmati AR, Töpel M, Patel R, Murtaza G, Jarvis P - Molecular and genetic analyses of Tic20 homologues in Arabidopsis thaliana chloroplasts
Visit the PubMed ArticleTo further characterize the chloroplast developmental defect in the tic20-I mutants, levels of several chloroplast proteins were assessed by immunoblotting ... Dramatic effects were observed in relation to components of the photosynthetic apparatus (LHCP ... with amounts reduced to less than 10% of that seen in the wild type
Kasmati AR, Töpel M, Patel R, Murtaza G, Jarvis P - Molecular and genetic analyses of Tic20 homologues in Arabidopsis thaliana chloroplasts
Visit the PubMed ArticleTo further characterize the chloroplast developmental defect in the tic20-I mutants, levels of several chloroplast proteins were assessed by immunoblotting ... In addition, the tetrapyrrole biosynthetic enzyme, CPO, was also strongly depleted in the tic20 mutants
Kasmati AR, Töpel M, Patel R, Murtaza G, Jarvis P - Molecular and genetic analyses of Tic20 homologues in Arabidopsis thaliana chloroplasts
Visit the PubMed ArticleTo corroborate the aforementioned conclusions, publicly available microarray data were analysed using the Genevestigator tool (Zimmermann et al., 2004). This revealed that three of the four Arabidopsis Tic20 genes are expressed throughout the plant’s life-cycle
Kasmati AR, Töpel M, Patel R, Murtaza G, Jarvis P - Molecular and genetic analyses of Tic20 homologues in Arabidopsis thaliana chloroplasts
Visit the PubMed ArticleThe two Group 2 genes (atTIC20-II and atTIC20-V) were expressed at relatively high levels throughout development, and shared similar patterns of expression (Figure 3); the numbers of ESTs detected were 92 and 95, respectively
Kasmati AR, Töpel M, Patel R, Murtaza G, Jarvis P - Molecular and genetic analyses of Tic20 homologues in Arabidopsis thaliana chloroplasts
Visit the PubMed ArticleatTic20-I (At1g04940 ... the subcellular localization of the Arabidopsis Tic20 homologues was assessed by analysing yellow fluorescent protein (YFP) fusion proteins ... the yellow-green fluorescence of YFP was observed in a ring-like pattern around the periphery of each chloroplast. This pattern was strongly reminiscent of the distributions seen for atTic110:YFP and atTic40:YFP fusion proteins in a previous study (Bédard et al., 2007), indicating that the Arabidopsis Tic20 homologues are all targeted to the chloroplast envelope
Kasmati AR, Töpel M, Patel R, Murtaza G, Jarvis P - Molecular and genetic analyses of Tic20 homologues in Arabidopsis thaliana chloroplasts
Visit the PubMed ArticleThe expression pattern of atTIC20-IV was different, and somewhat complementary to that of the other Group 1 gene, with low levels in rosette leaves and relatively high levels in non-photosynthetic tissues
Kasmati AR, Töpel M, Patel R, Murtaza G, Jarvis P - Molecular and genetic analyses of Tic20 homologues in Arabidopsis thaliana chloroplasts
Visit the PubMed ArticleThe two Group 2 genes (atTIC20-II and atTIC20-V) were expressed at relatively high levels throughout development, and shared similar patterns of expression (Figure 3); the numbers of ESTs detected were 92 and 95, respectively
Kasmati AR, Töpel M, Patel R, Murtaza G, Jarvis P - Molecular and genetic analyses of Tic20 homologues in Arabidopsis thaliana chloroplasts
Visit the PubMed Articleusing plants carrying RID2p::RID2:GFP ... Observation of fluorescence signals from RID2:GFP revealed that the RID2 protein is localized in nuclei, and that it accumulates mainly in nucleoli (Figure 10); this is consistent with the presence of an NLS
Ohbayashi I, Konishi M, Ebine K, Sugiyama M - Genetic identification of Arabidopsis RID2 as an essential factor involved in pre-rRNA processing
Visit the PubMed ArticleSpatial patterns of RID2 expression were examined by using RID2p::GUS as a reporter gene. In seedlings, a high level of GUS expression was observed ... in stipules
Ohbayashi I, Konishi M, Ebine K, Sugiyama M - Genetic identification of Arabidopsis RID2 as an essential factor involved in pre-rRNA processing
Visit the PubMed ArticleSpatial patterns of RID2 expression were examined by using RID2p::GUS as a reporter gene. In seedlings, a high level of GUS expression was observed ... in developing trichomes
Ohbayashi I, Konishi M, Ebine K, Sugiyama M - Genetic identification of Arabidopsis RID2 as an essential factor involved in pre-rRNA processing
Visit the PubMed ArticleAtHsp70-1 (At5g02500 ... localize to the ... nucleus
Jungkunz I, Link K, Vogel F, Voll LM, Sonnewald S, Sonnewald U - AtHsp70-15-deficient Arabidopsis plants are characterized by reduced growth, a constitutive cytosolic protein response and enhanced resistance to TuMV
Visit the PubMed ArticleMicroarray analysis was performed using the Agilent Arabidopsis IV microarray chip ... hsp70-15 knockout plants ... one DnaJ protein transcript were found to be significantly down-regulated
Jungkunz I, Link K, Vogel F, Voll LM, Sonnewald S, Sonnewald U - AtHsp70-15-deficient Arabidopsis plants are characterized by reduced growth, a constitutive cytosolic protein response and enhanced resistance to TuMV
Visit the PubMed ArticleMicroarray analysis was performed using the Agilent Arabidopsis IV microarray chip ... hsp70-15 knockout plants ... WRKY58 ... strongly down-regulated
Jungkunz I, Link K, Vogel F, Voll LM, Sonnewald S, Sonnewald U - AtHsp70-15-deficient Arabidopsis plants are characterized by reduced growth, a constitutive cytosolic protein response and enhanced resistance to TuMV
Visit the PubMed ArticleAtHsp70-15 ... target GFP to the ... nucleus
Jungkunz I, Link K, Vogel F, Voll LM, Sonnewald S, Sonnewald U - AtHsp70-15-deficient Arabidopsis plants are characterized by reduced growth, a constitutive cytosolic protein response and enhanced resistance to TuMV
Visit the PubMed ArticleMicroarray analysis was performed using the Agilent Arabidopsis IV microarray chip ... amiRNA-AtHsp70-14/15 line 4 ... WRKY22 ... strongly down-regulated
Jungkunz I, Link K, Vogel F, Voll LM, Sonnewald S, Sonnewald U - AtHsp70-15-deficient Arabidopsis plants are characterized by reduced growth, a constitutive cytosolic protein response and enhanced resistance to TuMV
Visit the PubMed ArticleAtHsp70-14 ... target GFP to the ... nucleus
Jungkunz I, Link K, Vogel F, Voll LM, Sonnewald S, Sonnewald U - AtHsp70-15-deficient Arabidopsis plants are characterized by reduced growth, a constitutive cytosolic protein response and enhanced resistance to TuMV
Visit the PubMed ArticleAtHsp70-14 (At1g79930) and AtHsp70-15 (At1g79920 ... Due to their high sequence similarities, they are represented by only one oligonucleotide (array identifier 262054_s_at) on the Affymetrix ATH1 microarray chip. In silico expression analysis using publicly available microarray data revealed that either or both of the genes are expressed ubiquitously in nearly all organs and at all developmental stages, with highest expression levels in callus and cell suspension cultures. In addition, the transcript signal is up-regulated after heat shock
Jungkunz I, Link K, Vogel F, Voll LM, Sonnewald S, Sonnewald U - AtHsp70-15-deficient Arabidopsis plants are characterized by reduced growth, a constitutive cytosolic protein response and enhanced resistance to TuMV
Visit the PubMed ArticleAtHsp70-2 (At5g02490 ... To analyse the subcellular localization ... GFP was fused to their full-length coding sequences. The construct was placed under the control of the constitutive CaMV 35S promoter as described in Experimental Procedures, and was stably transformed into Nicotiana tabacum plants using Agrobacterium-mediated gene transfer. Of the transformants ... 12 individuals with detectable GFP fluorescence (presumably in the cytosol and the nucleus) were obtained, and three of them were analysed by confocal laser scanning microscopy. As evident from the confocal laser scanning microscopy images shown in Figure 1(a,b), both isoforms localize to the cytosol and the nucleus. To exclude the possibility of proteolytic breakdown of the fusion proteins, immunoblot analysis using anti-GFP-specific antibodies was performed. As shown in Figure 1(c), GFP-specific antibodies recognize a single protein band of approximately 110 kDa in protein extracts from plants expressing ... GFP–AtHsp70-2, corresponding to the size of the fusion protein
Jungkunz I, Link K, Vogel F, Voll LM, Sonnewald S, Sonnewald U - AtHsp70-15-deficient Arabidopsis plants are characterized by reduced growth, a constitutive cytosolic protein response and enhanced resistance to TuMV
Visit the PubMed ArticleAtHsp70-14 (At1g79930) and AtHsp70-15 (At1g79920) ... Due to their high sequence similarities, they are represented by only one oligonucleotide (array identifier 262054_s_at) on the Affymetrix ATH1 microarray chip. In silico expression analysis using publicly available microarray data revealed that either or both of the genes are expressed ubiquitously in nearly all organs and at all developmental stages, with highest expression levels in callus and cell suspension cultures. In addition, the transcript signal is up-regulated after heat shock
Jungkunz I, Link K, Vogel F, Voll LM, Sonnewald S, Sonnewald U - AtHsp70-15-deficient Arabidopsis plants are characterized by reduced growth, a constitutive cytosolic protein response and enhanced resistance to TuMV
Visit the PubMed ArticleAtHsp70-1 (At5g02500) ... To analyse the subcellular localization ... GFP was fused to their full-length coding sequences. The construct was placed under the control of the constitutive CaMV 35S promoter as described in Experimental Procedures, and was stably transformed into Nicotiana tabacum plants using Agrobacterium-mediated gene transfer. Of the transformants, 20 ... individuals with detectable GFP fluorescence (presumably in the cytosol and the nucleus) were obtained, and three of them were analysed by confocal laser scanning microscopy. As evident from the confocal laser scanning microscopy images shown in Figure 1(a,b), both isoforms localize to the cytosol and the nucleus. To exclude the possibility of proteolytic breakdown of the fusion proteins, immunoblot analysis using anti-GFP-specific antibodies was performed. As shown in Figure 1(c), GFP-specific antibodies recognize a single protein band of approximately 110 kDa in protein extracts from plants expressing GFP–AtHsp70-1 ... corresponding to the size of the fusion protein
Jungkunz I, Link K, Vogel F, Voll LM, Sonnewald S, Sonnewald U - AtHsp70-15-deficient Arabidopsis plants are characterized by reduced growth, a constitutive cytosolic protein response and enhanced resistance to TuMV
Visit the PubMed ArticleMicroarray analysis was performed using the Agilent Arabidopsis IV microarray chip ... hsp70-15 knockout plants ... WRKY22 ... strongly down-regulated
Jungkunz I, Link K, Vogel F, Voll LM, Sonnewald S, Sonnewald U - AtHsp70-15-deficient Arabidopsis plants are characterized by reduced growth, a constitutive cytosolic protein response and enhanced resistance to TuMV
Visit the PubMed ArticleMicroarray analysis was performed using the Agilent Arabidopsis IV microarray chip ... amiRNA-AtHsp70-14/15 line 4 ... genes involved in lipid binding ... LTP2 ... were also found to be up-regulated
Jungkunz I, Link K, Vogel F, Voll LM, Sonnewald S, Sonnewald U - AtHsp70-15-deficient Arabidopsis plants are characterized by reduced growth, a constitutive cytosolic protein response and enhanced resistance to TuMV
Visit the PubMed ArticleMicroarray analysis was performed using the Agilent Arabidopsis IV microarray chip ... amiRNA-AtHsp70-14/15 line 4 ... WRKY58 ... strongly down-regulated
Jungkunz I, Link K, Vogel F, Voll LM, Sonnewald S, Sonnewald U - AtHsp70-15-deficient Arabidopsis plants are characterized by reduced growth, a constitutive cytosolic protein response and enhanced resistance to TuMV
Visit the PubMed ArticleMicroarray analysis was performed using the Agilent Arabidopsis IV microarray chip ... amiRNA-AtHsp70-14/15 line 4 ... genes involved in lipid binding ... LTP3 ... were also found to be up-regulated
Jungkunz I, Link K, Vogel F, Voll LM, Sonnewald S, Sonnewald U - AtHsp70-15-deficient Arabidopsis plants are characterized by reduced growth, a constitutive cytosolic protein response and enhanced resistance to TuMV
Visit the PubMed ArticleTo analyse the subcellular localization of AtHsp70-14 ... GFP was fused to the C-terminal end of the full-length coding regions ... cloned under the control of the constitutive CaMV 35S promoter, and transiently expressed in Nicotiana benthamiana. As shown in Figure 2 ... genes target GFP to the cytosol and nucleus
Jungkunz I, Link K, Vogel F, Voll LM, Sonnewald S, Sonnewald U - AtHsp70-15-deficient Arabidopsis plants are characterized by reduced growth, a constitutive cytosolic protein response and enhanced resistance to TuMV
Visit the PubMed ArticleMicroarray analysis was performed using the Agilent Arabidopsis IV microarray chip ... hsp70-15 knockout plants ... WRKY40 ... strongly down-regulated
Jungkunz I, Link K, Vogel F, Voll LM, Sonnewald S, Sonnewald U - AtHsp70-15-deficient Arabidopsis plants are characterized by reduced growth, a constitutive cytosolic protein response and enhanced resistance to TuMV
Visit the PubMed ArticleMicroarray analysis was performed using the Agilent Arabidopsis IV microarray chip ... amiRNA-AtHsp70-14/15 line 4 ... WRKY40 ... strongly down-regulated
Jungkunz I, Link K, Vogel F, Voll LM, Sonnewald S, Sonnewald U - AtHsp70-15-deficient Arabidopsis plants are characterized by reduced growth, a constitutive cytosolic protein response and enhanced resistance to TuMV
Visit the PubMed ArticleMicroarray analysis was performed using the Agilent Arabidopsis IV microarray chip ... amiRNA-AtHsp70-14/15 line 4 ... one DnaJ protein transcript were found to be significantly down-regulated
Jungkunz I, Link K, Vogel F, Voll LM, Sonnewald S, Sonnewald U - AtHsp70-15-deficient Arabidopsis plants are characterized by reduced growth, a constitutive cytosolic protein response and enhanced resistance to TuMV
Visit the PubMed ArticleMicroarray analysis was performed using the Agilent Arabidopsis IV microarray chip ... amiRNA-AtHsp70-14/15 line 4 ... WRKY38 ... strongly down-regulated
Jungkunz I, Link K, Vogel F, Voll LM, Sonnewald S, Sonnewald U - AtHsp70-15-deficient Arabidopsis plants are characterized by reduced growth, a constitutive cytosolic protein response and enhanced resistance to TuMV
Visit the PubMed ArticleMicroarray analysis was performed using the Agilent Arabidopsis IV microarray chip ... hsp70-15 knockout plants ... genes involved in lipid binding ... LTP2 ... were also found to be up-regulated
Jungkunz I, Link K, Vogel F, Voll LM, Sonnewald S, Sonnewald U - AtHsp70-15-deficient Arabidopsis plants are characterized by reduced growth, a constitutive cytosolic protein response and enhanced resistance to TuMV
Visit the PubMed ArticleMicroarray analysis was performed using the Agilent Arabidopsis IV microarray chip ... hsp70-15 knockout plants ... genes involved in lipid binding ... LTP3 ... were also found to be up-regulated
Jungkunz I, Link K, Vogel F, Voll LM, Sonnewald S, Sonnewald U - AtHsp70-15-deficient Arabidopsis plants are characterized by reduced growth, a constitutive cytosolic protein response and enhanced resistance to TuMV
Visit the PubMed ArticleAtHsp70-2 (At5g02490) ... localize to the ... nucleus
Jungkunz I, Link K, Vogel F, Voll LM, Sonnewald S, Sonnewald U - AtHsp70-15-deficient Arabidopsis plants are characterized by reduced growth, a constitutive cytosolic protein response and enhanced resistance to TuMV
Visit the PubMed ArticleMicroarray analysis was performed using the Agilent Arabidopsis IV microarray chip ... hsp70-15 knockout plants ... WRKY38 ... strongly down-regulated
Jungkunz I, Link K, Vogel F, Voll LM, Sonnewald S, Sonnewald U - AtHsp70-15-deficient Arabidopsis plants are characterized by reduced growth, a constitutive cytosolic protein response and enhanced resistance to TuMV
Visit the PubMed ArticleTo analyse the subcellular localization of ... AtHsp70-15, GFP was fused to the C-terminal end of the full-length coding regions ... cloned under the control of the constitutive CaMV 35S promoter, and transiently expressed in Nicotiana benthamiana. As shown in Figure 2 ... genes target GFP to the cytosol and nucleus
Jungkunz I, Link K, Vogel F, Voll LM, Sonnewald S, Sonnewald U - AtHsp70-15-deficient Arabidopsis plants are characterized by reduced growth, a constitutive cytosolic protein response and enhanced resistance to TuMV
Visit the PubMed ArticleWild-type (Col-0) Arabidopsis leaves develop an ovate form with a limited number of serrations towards the proximal base of the leaf (Figure 2a). At a very early stage of leaf development, the region of future perimeter deformation is defined by CUC2 expression (as visualized by CUC2>>GUS reporter gene activity (Figure S1a; Nikovics et al., 2006). CUC2 expression then gradually becomes restricted to regions on the leaf perimeter adjacent to the emerging lobe
Malinowski R, Kasprzewska A, Fleming AJ - Targeted manipulation of leaf form via local growth repression
Visit the PubMed Articlepromoter-driven GUS activity patterns ... BPC4 ... promoter ... active in the center of young rosettes and in young rosette leaves
Monfared MM, Simon MK, Meister RJ, Roig-Villanova I, Kooiker M, Colombo L, Fletcher JC, Gasser CS - Overlapping and antagonistic activities of BASIC PENTACYSTEINE genes affect a range of developmental processes in Arabidopsis
Visit the PubMed Articlepromoter-driven GUS activity patterns ... BPC6 promoter ... active in the center of young rosettes and in young rosette leaves
Monfared MM, Simon MK, Meister RJ, Roig-Villanova I, Kooiker M, Colombo L, Fletcher JC, Gasser CS - Overlapping and antagonistic activities of BASIC PENTACYSTEINE genes affect a range of developmental processes in Arabidopsis
Visit the PubMed ArticleBPC7 promoter activity was limited to the leaf vasculature
Monfared MM, Simon MK, Meister RJ, Roig-Villanova I, Kooiker M, Colombo L, Fletcher JC, Gasser CS - Overlapping and antagonistic activities of BASIC PENTACYSTEINE genes affect a range of developmental processes in Arabidopsis
Visit the PubMed Articlepromoter-driven GUS activity patterns ... BPC1 ... promoter activity was limited to the leaf vasculature
Monfared MM, Simon MK, Meister RJ, Roig-Villanova I, Kooiker M, Colombo L, Fletcher JC, Gasser CS - Overlapping and antagonistic activities of BASIC PENTACYSTEINE genes affect a range of developmental processes in Arabidopsis
Visit the PubMed Articlepromoter-driven GUS activity patterns ... BPC2 ... promoter ... active in the center of young rosettes and in young rosette leaves
Monfared MM, Simon MK, Meister RJ, Roig-Villanova I, Kooiker M, Colombo L, Fletcher JC, Gasser CS - Overlapping and antagonistic activities of BASIC PENTACYSTEINE genes affect a range of developmental processes in Arabidopsis
Visit the PubMed Articlepromoter-driven GUS activity patterns ... BPC3 ... promoter ... active in the center of young rosettes and in young rosette leaves
Monfared MM, Simon MK, Meister RJ, Roig-Villanova I, Kooiker M, Colombo L, Fletcher JC, Gasser CS - Overlapping and antagonistic activities of BASIC PENTACYSTEINE genes affect a range of developmental processes in Arabidopsis
Visit the PubMed Articlewe screened for genes that were up-regulated in plants expressing CUC1 under the control of the CaMV 35S promoter (35S:CUC1; Takada et al., 2001) by transcriptome analysis. We found that the expression levels of 15 genes were increased more than eight-fold in 35S:CUC1 compared with wild-type (Table S1). Of these, we focused on At3g23290 ... Up-regulation of this gene in 35S:CUC1 was confirmed by RT-PCR analysis
Takeda S, Hanano K, Kariya A, Shimizu S, Zhao L, Matsui M, Tasaka M, Aida M - CUP-SHAPED COTYLEDON1 transcription factor activates the expression of LSH4 and LSH3, two members of the ALOG gene family, in shoot organ boundary cells
Visit the PubMed Articlewe generated transgenic plants carrying a translational fusion of the LSH4 genomic fragment and green fluorescent protein (LSH4g:GFP). The fusion protein accumulated in cells between two cotyledon primordia in the embryos, and no GFP signal was found in the cells in the central region
Takeda S, Hanano K, Kariya A, Shimizu S, Zhao L, Matsui M, Tasaka M, Aida M - CUP-SHAPED COTYLEDON1 transcription factor activates the expression of LSH4 and LSH3, two members of the ALOG gene family, in shoot organ boundary cells
Visit the PubMed ArticleIn 4-day-old seedlings, expression of LSH3 was detected in cells between the SAM and the cotyledons
Takeda S, Hanano K, Kariya A, Shimizu S, Zhao L, Matsui M, Tasaka M, Aida M - CUP-SHAPED COTYLEDON1 transcription factor activates the expression of LSH4 and LSH3, two members of the ALOG gene family, in shoot organ boundary cells
Visit the PubMed ArticleAfter germination, the LSH4–GFP fusion protein accumulated in boundary cells between the two cotyledons (Figure 1m) and in cells between the shoot meristem and the leaf primordia (Figure 1n), but not in the SAM
Takeda S, Hanano K, Kariya A, Shimizu S, Zhao L, Matsui M, Tasaka M, Aida M - CUP-SHAPED COTYLEDON1 transcription factor activates the expression of LSH4 and LSH3, two members of the ALOG gene family, in shoot organ boundary cells
Visit the PubMed ArticleRPS5Ap:5mCUC1-GR ... To investigate whether CUC1 can directly activate the transcription of LSH4, we analyzed its expression in homozygous plants treated with the protein synthesis inhibitor cycloheximide (CHX) together with DEX. Application of CHX efficiently blocks protein synthesis from transcripts of CUC1 primary response genes, thereby inhibiting induction of genes that are indirectly activated by primary response gene products (Sablowski and Meyerowitz, 1998). The effect of CHX was confirmed by expression of IAA5, a CHX-induced gene (data not shown; Abel et al., 1995). In the presence of CHX, expression of LSH4 was effectively induced after 3 h of DEX treatment ... These data show that CUC1 activates transcription of LSH4 ... under conditions in which protein synthesis is inhibited, which in turn indicates that LSH4 ... direct transcriptional targets of CUC1
Takeda S, Hanano K, Kariya A, Shimizu S, Zhao L, Matsui M, Tasaka M, Aida M - CUP-SHAPED COTYLEDON1 transcription factor activates the expression of LSH4 and LSH3, two members of the ALOG gene family, in shoot organ boundary cells
Visit the PubMed ArticleIn cuc1 cuc2 double mutants, the GUS activity at the cotyledon boundary was dramatically reduced, but that in the root tip was not affected (Figure 2c). In situ hybridization showed that LSH3 was weakly expressed in the boundary cells between the SAM and the cotyledons in bending-cotyledon stage embryos
Takeda S, Hanano K, Kariya A, Shimizu S, Zhao L, Matsui M, Tasaka M, Aida M - CUP-SHAPED COTYLEDON1 transcription factor activates the expression of LSH4 and LSH3, two members of the ALOG gene family, in shoot organ boundary cells
Visit the PubMed ArticleLSH4 was initially expressed at the shoot apex in the early-heart embryonic stage (Figure 1a). Within the apex of the late-heart stage embryo, the signal was detected in the peripheral region but not in the central region (Figure 1b,c), indicating that LSH4 expression partially overlaps with expression of CUC1 at this stage (Aida et al., 1999; Takada et al., 2001). At the bent-cotyledon stage, LSH4 is expressed in boundary cells located between the SAM and the cotyledon primordia
Takeda S, Hanano K, Kariya A, Shimizu S, Zhao L, Matsui M, Tasaka M, Aida M - CUP-SHAPED COTYLEDON1 transcription factor activates the expression of LSH4 and LSH3, two members of the ALOG gene family, in shoot organ boundary cells
Visit the PubMed Articlethe LSH4–GFP ... fusion protein weakly accumulated in early leaf primordia, but not in those at later stages (Video Clip S2). It also accumulated in cells at the boundary of the meristem and stem
Takeda S, Hanano K, Kariya A, Shimizu S, Zhao L, Matsui M, Tasaka M, Aida M - CUP-SHAPED COTYLEDON1 transcription factor activates the expression of LSH4 and LSH3, two members of the ALOG gene family, in shoot organ boundary cells
Visit the PubMed Articlethe LSH4–GFP ... fusion protein always localized to the nucleus
Takeda S, Hanano K, Kariya A, Shimizu S, Zhao L, Matsui M, Tasaka M, Aida M - CUP-SHAPED COTYLEDON1 transcription factor activates the expression of LSH4 and LSH3, two members of the ALOG gene family, in shoot organ boundary cells
Visit the PubMed ArticleRPS5Ap:5mCUC1-GR ... To investigate whether CUC1 can directly activate the transcription of LSH4, we analyzed its expression in homozygous plants treated with the protein synthesis inhibitor cycloheximide (CHX) together with DEX. Application of CHX efficiently blocks protein synthesis from transcripts of CUC1 primary response genes, thereby inhibiting induction of genes that are indirectly activated by primary response gene products (Sablowski and Meyerowitz, 1998). The effect of CHX was confirmed by expression of IAA5, a CHX-induced gene (data not shown; Abel et al., 1995). In the presence of CHX ... LSH3 expression was induced ... These data show that CUC1 activates transcription of ... LSH3 under conditions in which protein synthesis is inhibited, which in turn indicates that ... LSH3 ... direct transcriptional targets of CUC1
Takeda S, Hanano K, Kariya A, Shimizu S, Zhao L, Matsui M, Tasaka M, Aida M - CUP-SHAPED COTYLEDON1 transcription factor activates the expression of LSH4 and LSH3, two members of the ALOG gene family, in shoot organ boundary cells
Visit the PubMed ArticleFirst, we examined the LSH4 expression in RPS5Ap:5mCUC1-GR homozygous plants grown on agar medium for 3 days and then transplanted to medium containing 1 μm DEX. The level of LSH4 expression increased two- to three-fold after 24 h (Figure S2). When the plants were grown on medium without DEX, the LSH4 transcript levels did not increase. Control plants expressing yeast GAL4 under the control of the RPS5A promoter (Weijers et al., 2001; Aida et al., 2004) showed no induction of LSH4 expression after DEX treatment (Figure S2
Takeda S, Hanano K, Kariya A, Shimizu S, Zhao L, Matsui M, Tasaka M, Aida M - CUP-SHAPED COTYLEDON1 transcription factor activates the expression of LSH4 and LSH3, two members of the ALOG gene family, in shoot organ boundary cells
Visit the PubMed ArticleA LSH3–GFP fusion protein localized to the nucleus when it was expressed under the control of the 35S promoter
Takeda S, Hanano K, Kariya A, Shimizu S, Zhao L, Matsui M, Tasaka M, Aida M - CUP-SHAPED COTYLEDON1 transcription factor activates the expression of LSH4 and LSH3, two members of the ALOG gene family, in shoot organ boundary cells
Visit the PubMed ArticleIn heart-stage embryos, the LSH3 promoter was active in the cotyledon boundary region of the shoot apex
Takeda S, Hanano K, Kariya A, Shimizu S, Zhao L, Matsui M, Tasaka M, Aida M - CUP-SHAPED COTYLEDON1 transcription factor activates the expression of LSH4 and LSH3, two members of the ALOG gene family, in shoot organ boundary cells
Visit the PubMed ArticleLSH4 transcripts were not detected in cup-shaped embryos segregating from cuc1 cuc2/+ parents (Figure 1e), but were detected in siblings with normal appearance, as in wild-type
Takeda S, Hanano K, Kariya A, Shimizu S, Zhao L, Matsui M, Tasaka M, Aida M - CUP-SHAPED COTYLEDON1 transcription factor activates the expression of LSH4 and LSH3, two members of the ALOG gene family, in shoot organ boundary cells
Visit the PubMed ArticleFour days after germination, LSH4 was weakly expressed in the basal cells of the leaf primordia (Figure 1f). Essentially the same expression pattern was observed in a histochemical analysis of transgenic plants expressing the β-glucuronidase reporter gene (GUS) under the control of the 1.5 kb LSH4 promoter (inset to Figure 1f), suggesting that this region contains cis-regulatory elements for the LSH4 expression
Takeda S, Hanano K, Kariya A, Shimizu S, Zhao L, Matsui M, Tasaka M, Aida M - CUP-SHAPED COTYLEDON1 transcription factor activates the expression of LSH4 and LSH3, two members of the ALOG gene family, in shoot organ boundary cells
Visit the PubMed ArticleAt3g23290 ... is expressed in the shoot apex of embryos
Takeda S, Hanano K, Kariya A, Shimizu S, Zhao L, Matsui M, Tasaka M, Aida M - CUP-SHAPED COTYLEDON1 transcription factor activates the expression of LSH4 and LSH3, two members of the ALOG gene family, in shoot organ boundary cells
Visit the PubMed Articlespa1 spa2 spa3 mutant background ... ML1 promoters conferred GUS-SPA1 expression specifically in the ... epidermis
Ranjan A, Fiene G, Fackendahl P, Hoecker U - The Arabidopsis repressor of light signaling SPA1 acts in the phloem to regulate seedling de-etiolation, leaf expansion and flowering time
Visit the PubMed ArticleThe spatial SPA1 transcript accumulation was very similar in the spa mutant backgrounds
Ranjan A, Fiene G, Fackendahl P, Hoecker U - The Arabidopsis repressor of light signaling SPA1 acts in the phloem to regulate seedling de-etiolation, leaf expansion and flowering time
Visit the PubMed Articletransgenic plants expressing a GUS-SPA1 fusion protein under the control of the native SPA1 promoter (SPA1::GUS-SPA1 ... mutant backgrounds ... spa1 spa2 spa3 triple mutant ... In seedlings ... GUS-SPA1 accumulated ... in vascular bundles
Ranjan A, Fiene G, Fackendahl P, Hoecker U - The Arabidopsis repressor of light signaling SPA1 acts in the phloem to regulate seedling de-etiolation, leaf expansion and flowering time
Visit the PubMed Articletransgenic plants expressing a GUS-SPA1 fusion protein under the control of the native SPA1 promoter (SPA1::GUS-SPA1 ... mutant backgrounds ... spa1 spa2 spa3 triple mutant ... In seedlings ... GUS-SPA1 accumulated in the ... mesophyll
Ranjan A, Fiene G, Fackendahl P, Hoecker U - The Arabidopsis repressor of light signaling SPA1 acts in the phloem to regulate seedling de-etiolation, leaf expansion and flowering time
Visit the PubMed Articletransgenic plants expressing a GUS-SPA1 fusion protein under the control of the native SPA1 promoter (SPA1::GUS-SPA1 ... mutant backgrounds ... spa1 spa3 spa4 mutant ... in adult plants, GUS-SPA1 ... expression in vascular bundles
Ranjan A, Fiene G, Fackendahl P, Hoecker U - The Arabidopsis repressor of light signaling SPA1 acts in the phloem to regulate seedling de-etiolation, leaf expansion and flowering time
Visit the PubMed Articlespa1 spa2 spa3 mutant background ... CAB3 ... promoters conferred GUS-SPA1 expression specifically in the mesophyll
Ranjan A, Fiene G, Fackendahl P, Hoecker U - The Arabidopsis repressor of light signaling SPA1 acts in the phloem to regulate seedling de-etiolation, leaf expansion and flowering time
Visit the PubMed Articlespa1 spa3 spa4 mutants ... background ... ML1::GUS-SPA1 ... expression levels ... at the shoot apex
Ranjan A, Fiene G, Fackendahl P, Hoecker U - The Arabidopsis repressor of light signaling SPA1 acts in the phloem to regulate seedling de-etiolation, leaf expansion and flowering time
Visit the PubMed Articlespa1 spa2 spa3 mutant background ... RolC promoter in ... light-grown seedlings ... caused lower levels of GUS-SPA1 accumulation than did the SPA1 promoter
Ranjan A, Fiene G, Fackendahl P, Hoecker U - The Arabidopsis repressor of light signaling SPA1 acts in the phloem to regulate seedling de-etiolation, leaf expansion and flowering time
Visit the PubMed Articlespa1 spa2 spa3 mutant background ... RolC promoter in dark ... grown seedlings ... caused lower levels of GUS-SPA1 accumulation than did the SPA1 promoter
Ranjan A, Fiene G, Fackendahl P, Hoecker U - The Arabidopsis repressor of light signaling SPA1 acts in the phloem to regulate seedling de-etiolation, leaf expansion and flowering time
Visit the PubMed Articlespa1 spa3 spa4 ... background ... In CAB3::GUS-SPA1 transgenic lines, GUS-SPA1 expression was restricted to mesophyll cells
Ranjan A, Fiene G, Fackendahl P, Hoecker U - The Arabidopsis repressor of light signaling SPA1 acts in the phloem to regulate seedling de-etiolation, leaf expansion and flowering time
Visit the PubMed Articlespa1 spa2 spa3 mutant background ... Transgenic SUC2::GUS-SPA1 lines also accumulated GUS specifically in the vasculature of light-grown seedlings
Ranjan A, Fiene G, Fackendahl P, Hoecker U - The Arabidopsis repressor of light signaling SPA1 acts in the phloem to regulate seedling de-etiolation, leaf expansion and flowering time
Visit the PubMed Articlespa1 spa2 spa3 mutant background ... The RolC promoter conferred vascular-specific expression of GUS-SPA1 in ... light-grown seedlings
Ranjan A, Fiene G, Fackendahl P, Hoecker U - The Arabidopsis repressor of light signaling SPA1 acts in the phloem to regulate seedling de-etiolation, leaf expansion and flowering time
Visit the PubMed Articlespa1 spa3 spa4 mutants ... background ... KNAT1::GUS-SPA1 ... shoot meristem ... expression of GUS-SPA1
Ranjan A, Fiene G, Fackendahl P, Hoecker U - The Arabidopsis repressor of light signaling SPA1 acts in the phloem to regulate seedling de-etiolation, leaf expansion and flowering time
Visit the PubMed Articlespa1 spa2 spa3 mutant background ... KNAT1::GUS-SPA1 ... transgenic lines showed shoot meristem ... specific expression of GUS-SPA1
Ranjan A, Fiene G, Fackendahl P, Hoecker U - The Arabidopsis repressor of light signaling SPA1 acts in the phloem to regulate seedling de-etiolation, leaf expansion and flowering time
Visit the PubMed Articlespa1 spa3 spa4 mutants ... background ... Exclusive expression of GUS-SPA1 in the epidermis was detected in ML1::GUS-SPA1
Ranjan A, Fiene G, Fackendahl P, Hoecker U - The Arabidopsis repressor of light signaling SPA1 acts in the phloem to regulate seedling de-etiolation, leaf expansion and flowering time
Visit the PubMed Articlespa1 spa2 spa3 mutant background ... The RolC promoter conferred vascular-specific expression of GUS-SPA1 in ... dark ... grown seedlings
Ranjan A, Fiene G, Fackendahl P, Hoecker U - The Arabidopsis repressor of light signaling SPA1 acts in the phloem to regulate seedling de-etiolation, leaf expansion and flowering time
Visit the PubMed ArticleIn developing leaves, SPA1 mRNA ... in inner tissues
Ranjan A, Fiene G, Fackendahl P, Hoecker U - The Arabidopsis repressor of light signaling SPA1 acts in the phloem to regulate seedling de-etiolation, leaf expansion and flowering time
Visit the PubMed Articletransgenic plants expressing a GUS-SPA1 fusion protein under the control of the native SPA1 promoter (SPA1::GUS-SPA1 ... mutant backgrounds ... spa1 spa3 spa4 mutant ... in adult plants, GUS-SPA1 ... expression in the epidermis
Ranjan A, Fiene G, Fackendahl P, Hoecker U - The Arabidopsis repressor of light signaling SPA1 acts in the phloem to regulate seedling de-etiolation, leaf expansion and flowering time
Visit the PubMed ArticleIn developing leaves, SPA1 mRNA ... in epidermal tissue
Ranjan A, Fiene G, Fackendahl P, Hoecker U - The Arabidopsis repressor of light signaling SPA1 acts in the phloem to regulate seedling de-etiolation, leaf expansion and flowering time
Visit the PubMed Articlespa1 spa3 spa4 mutants ... background ... ML1::GUS-SPA1 ... expression levels ... in developed leaves
Ranjan A, Fiene G, Fackendahl P, Hoecker U - The Arabidopsis repressor of light signaling SPA1 acts in the phloem to regulate seedling de-etiolation, leaf expansion and flowering time
Visit the PubMed ArticleSPA1 transcript levels were particularly high in vascular tissue of dark-grown seedlings
Ranjan A, Fiene G, Fackendahl P, Hoecker U - The Arabidopsis repressor of light signaling SPA1 acts in the phloem to regulate seedling de-etiolation, leaf expansion and flowering time
Visit the PubMed Articletransgenic plants expressing a GUS-SPA1 fusion protein under the control of the native SPA1 promoter (SPA1::GUS-SPA1 ... mutant backgrounds ... spa1 spa2 spa3 triple mutant ... In seedlings, GUS activity ... in cotyledons ... cotyledons
Ranjan A, Fiene G, Fackendahl P, Hoecker U - The Arabidopsis repressor of light signaling SPA1 acts in the phloem to regulate seedling de-etiolation, leaf expansion and flowering time
Visit the PubMed Articlespa1 spa3 spa4 mutants ... background ... expression of GUS-SPA1 in the epidermis was detected in ... CER6::GUS-SPA1 ... lines
Ranjan A, Fiene G, Fackendahl P, Hoecker U - The Arabidopsis repressor of light signaling SPA1 acts in the phloem to regulate seedling de-etiolation, leaf expansion and flowering time
Visit the PubMed Articletransgenic plants expressing a GUS-SPA1 fusion protein under the control of the native SPA1 promoter (SPA1::GUS-SPA1 ... mutant backgrounds ... spa1 spa2 spa3 triple mutant ... In seedlings ... GUS-SPA1 accumulated in the epidermis
Ranjan A, Fiene G, Fackendahl P, Hoecker U - The Arabidopsis repressor of light signaling SPA1 acts in the phloem to regulate seedling de-etiolation, leaf expansion and flowering time
Visit the PubMed Articlespa1 spa3 spa4 mutants ... background ... ML1::GUS-SPA1 ... expression levels ... in leaf primordia
Ranjan A, Fiene G, Fackendahl P, Hoecker U - The Arabidopsis repressor of light signaling SPA1 acts in the phloem to regulate seedling de-etiolation, leaf expansion and flowering time
Visit the PubMed Articletransgenic plants expressing a GUS-SPA1 fusion protein under the control of the native SPA1 promoter (SPA1::GUS-SPA1 ... mutant backgrounds ... spa1 spa3 spa4 mutant ... in adult plants ... GUS-SPA1 was ... expressed in the phloem
Ranjan A, Fiene G, Fackendahl P, Hoecker U - The Arabidopsis repressor of light signaling SPA1 acts in the phloem to regulate seedling de-etiolation, leaf expansion and flowering time
Visit the PubMed Articlespa1 spa2 spa3 mutant background ... CAB3 promoter in dark-grown seedlings ... caused lower levels of GUS-SPA1 accumulation than did the SPA1 promoter
Ranjan A, Fiene G, Fackendahl P, Hoecker U - The Arabidopsis repressor of light signaling SPA1 acts in the phloem to regulate seedling de-etiolation, leaf expansion and flowering time
Visit the PubMed Articlespa1 spa3 spa4 mutants ... background ... ML1::GUS-SPA1 ... expression levels ... in young developing leaves
Ranjan A, Fiene G, Fackendahl P, Hoecker U - The Arabidopsis repressor of light signaling SPA1 acts in the phloem to regulate seedling de-etiolation, leaf expansion and flowering time
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... HCF ... 173 ... downregulated ... at 27 DAS
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... Genes induced at 21 DAS ... include ... dehydration-responsive genes such as ... ERD14
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... A surprising group of genes identified by this analysis is downregulated for most of the time course followed by a significant increase in expression at 35 or 37 DAS (clusters U8_1 and U9). This group is highly enriched for genes involved in the cytoskeleton (see Supplemental Figure 3B online), with members of the α-tubulin family (TUA2
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS (Figure 1C). However, leaf weight increased significantly between time points up to 25 DAS ... A surprising group of genes identified by this analysis is downregulated for most of the time course followed by a significant increase in expression at 35 or 37 DAS ... AURORA2
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... genes upregulated at 27 DAS due to increased expression ... autophagy gene ATG7
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis thaliana plant at each time point (Figure 1A), and samples were not pooled for any of the analyses. Arabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... Key genes involved in chlorophyll degradation ... SGR2 ... show enhanced expression during senescence ... increase in expression during early time points, leveling out between 25 and 29 DAS, followed by a sudden increase in expression after 29 DAS
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... genes upregulated at 27 DAS due to increased expression ... MC6
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleGenes induced at 21 DAS ... include ... dehydration-responsive genes such as ... ABF2
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... At 27 DAS, there is an interesting overrepresentation of genes involved in carotene metabolism ... CCD7
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... ABI1 ... upregulated at 25 DAS
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... By the later time points in this experiment, after 31 DAS, the senescing leaf becomes more and more heterogeneous, with some cells within the leaf being at a more advanced stage of senescence than others and more variability between biological replicates. This means that there is less clarity in the functions of different groupings of genes that are differentially expressed at each time point after 31 DAS. GO term enrichment analysis of the 31, 33, and 35 DAS groups combined ... ACC OXIDASE2 (ACO2) being upregulated late in senescence
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... HCF ... 152 ... downregulated ... at 27 DAS
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... at 23 DAS ... LUT ... 5 ... show a correlated drop in expression
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... Genes induced at 21 DAS ... include ... MPK7
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... gene clusters that show expression that declines at ... 31 ... includes the gene-encoding ... GLK1
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... Key genes involved in chlorophyll degradation, SGR1 ... show enhanced expression during senescence ... increase in expression during early time points, leveling out between 25 and 29 DAS, followed by a sudden increase in expression after 29 DAS
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... The ORE9/MAX2 gene also shows senescence-enhanced expression and is upregulated at 21 DAS
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... Many genes encoding TFs are first upregulated at 23 DAS, including ... ATAF2
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... at 23 DAS ... ARR4 ... drops
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... At ... 29 DAS ... upregulated genes, LACS6, encoding a long-chain acyl-CoA synthetase
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... Many genes encoding TFs are first upregulated at 23 DAS, including ... ATAF1
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... Genes induced at 21 DAS ... include ... dehydration-responsive genes such as ERD1
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... Genes induced at 21 DAS ... include ... LSD1, a zinc finger that monitors superoxide levels and regulates cell death
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... gene clusters that show expression that declines at ... 31 ... includes the gene-encoding TF GOLDEN2-LIKE (GLK2)
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... expression increases at 23 DAS ... MYC2
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... at 23 DAS ... LUT1 ... show a correlated drop in expression
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... AFP1 ... upregulated at 25 DAS
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... At the next time point (29 DAS ... upregulated genes ... including the well-characterized senescence-enhanced Cys protease SAG12
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... By the later time points in this experiment, after 31 DAS, the senescing leaf becomes more and more heterogeneous, with some cells within the leaf being at a more advanced stage of senescence than others and more variability between biological replicates. This means that there is less clarity in the functions of different groupings of genes that are differentially expressed at each time point after 31 DAS. GO term enrichment analysis of the 31, 33, and 35 DAS groups combined ... ethylene ETR1 ... being upregulated late in senescence
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... at 23 DAS ... LUT ... 2 ... show a correlated drop in expression
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... A surprising group of genes identified by this analysis is downregulated for most of the time course followed by a significant increase in expression at 35 or 37 DAS (clusters U8_1 and U9). This group is highly enriched for genes involved in the cytoskeleton ... with members of the ... actin genes ... ACT3
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... gene clusters that show expression that declines at ... 31 ... includes ... PSII chlorophyll binding proteins LHCB ... 4.2
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... senescence-enhanced gene SAG12 ... SAG12 expression first becomes significantly enhanced around 31 DAS. This method can also be used to show when the gene expression gradient is maximal, i.e., the time of most rapid change. For example, the maximum change of the expression for SAG12 occurs between 33 and 35
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... at 23 DAS ... ARR ... 6 ... drops
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... Key genes involved in chlorophyll degradation ... PaO ... show enhanced expression during senescence ... increase in expression during early time points, leveling out between 25 and 29 DAS, followed by a sudden increase in expression after 29 DAS
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... Genes induced at 21 DAS ... include ... DREB2A
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... A surprising group of genes identified by this analysis is downregulated for most of the time course followed by a significant increase in expression at 35 or 37 DAS (clusters U8_1 and U9). This group is highly enriched for genes involved in the cytoskeleton (see Supplemental Figure 3B online), with members of the α-tubulin family (TUA ... 5
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... expression increases at 23 DAS ... JAZ8
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... ATG8B ... show ... rapidly increased expression between 29 and 31 DAS
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... ATG8H ... show ... rapidly increased expression between 29 and 31 DAS
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... Genes induced at 21 DAS ... include ... dehydration-responsive genes such as ... RAB18
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... Key genes involved in chlorophyll degradation ... NYC1 ... show enhanced expression during senescence ... increase in expression during early time points, leveling out between 25 and 29 DAS, followed by a sudden increase in expression after 29 DAS
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... some JA biosynthesis and signaling genes are only expressed at the early time point (e.g., OPR3)
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... ATG7, being upregulated at 29 DAS ... show ... rapidly increased expression between 29 and 31 DAS
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... Many genes encoding TFs are first upregulated at 23 DAS, including ... Nuclear Factor Y subunit NF-YA4
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS (Figure 1C). However, leaf weight increased significantly between time points up to 25 DAS ... A surprising group of genes identified by this analysis is downregulated for most of the time course followed by a significant increase in expression at 35 or 37 DAS (clusters U8_1 and U9). This group is highly enriched for genes involved in the cytoskeleton ... with members of ... actin genes ... ACT11
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... at 23 DAS ... ARR ... 7 ... drops
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... expression increases at 23 DAS ... AOC4
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... HCF ... 208 ... downregulated ... at 27 DAS
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... HCF101 ... downregulated ... at 27 DAS
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... gene clusters that show expression that declines at ... 31 ... includes ... PSII chlorophyll binding proteins LHCB ... 6
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleGenes induced at 21 DAS ... include ... HSP70
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... At 27 DAS, there is an interesting overrepresentation of genes involved in carotene metabolism ... CCD8
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... expression increases at 23 DAS ... JAZ1
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... expression increases at 23 DAS ... JAZ6
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... A surprising group of genes identified by this analysis is downregulated for most of the time course followed by a significant increase in expression at 35 or 37 DAS ... AURORA1
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... upregulated genes ... ACX1 (acyl-CoA oxidase), encoding the enzyme that catalyzes the first step in fatty acid β-oxidation in the peroxisome
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... Genes induced at 21 DAS ... include ... Arabidopsis HISTIDINE KINASE3 (AHK3)
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... gene clusters that show expression that declines at ... 31 ... includes ... PSII chlorophyll binding proteins LHCB 2.2
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... ATG8A ... show ... rapidly increased expression between 29 and 31 DAS
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... Many genes encoding TFs are first upregulated at 23 DAS, including ... CUC2
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... HCF ... 109 ... downregulated ... at 27 DAS
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... expression increases at 23 DAS ... AOC1
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... A surprising group of genes identified by this analysis is downregulated for most of the time course followed by a significant increase in expression at 35 or 37 DAS (clusters U8_1 and U9). This group is highly enriched for genes involved in the cytoskeleton (see Supplemental Figure 3B online), with members of the α-tubulin family (TUA ... 4
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed ArticleArabidopsis Col-0 plants were grown in controlled conditions until leaf 7 was ~50% of its final size (19 d after sowing [DAS]). This leaf was harvested at defined time points until 39 DAS when it was visibly senescent (~50% of leaf area being yellow, Figure 1B). Samples were taken in the morning (7 h into the light period) and afternoon (14 h into the light period) every other day, resulting in 22 time points in total. Sampling was carried out at these two time points each day to allow us to distinguish genes that are altered in a diurnal rhythm, as well as being differentially expressed over time; the times were selected based on likely maximum changes in expression. Plants started flowering from around 21 DAS. Leaf 7 started to show yellowing at the tip at around 31 DAS and was 25 to 50% yellow by 37 DAS. By the final sample time (39 DAS), the plants were fully flowering, and siliques were filling. Physiological parameters were measured in the morning samples only (i.e., 11 time points). Sampled leaves reached full expansion by 23 DAS ... genes upregulated at 27 DAS due to increased expression ... MC9
Breeze E, Harrison E, McHattie S, Hughes L, Hickman R, Hill C, Kiddle S, Kim YS, Penfold CA, Jenkins D, Zhang C, Morris K, Jenner C, Jackson S, Thomas B, Tabrett A, Legaie R, Moore JD, Wild DL, Ott S, Rand D, Beynon J, Denby K, Mead A, Buchanan-Wollaston V - High-resolution temporal profiling of transcripts during Arabidopsis leaf senescence reveals a distinct chronology of processes and regulation
Visit the PubMed Articlewe selected three genes that were at least two-fold upregulated after 15 min of cytokinin treatment (GATA22, bHLH64 and HAT22 induced 2.0-, 2.9- and 2.4-fold, respectively) and one more gene that was strongly induced after 120 min of cytokinin treatment (HAT4, 13.1-fold) for further analysis ... The transcript level of all chosen genes was enhanced about two- to four-fold after 15 min or 120 min of cytokinin treatment (Fig. 1), confirming the results of the microarray analysis
Köllmer I, Werner T, Schmülling T - Ectopic expression of different cytokinin-regulated transcription factor genes of Arabidopsis thaliana alters plant growth and development
Visit the PubMed Articlewe selected three genes that were at least two-fold upregulated after 15 min of cytokinin treatment (GATA22, bHLH64 and HAT22 induced 2.0-, 2.9- and 2.4-fold, respectively) and one more gene that was strongly induced after 120 min of cytokinin treatment (HAT4, 13.1-fold) for further analysis ... The transcript level of all chosen genes was enhanced about two- to four-fold after 15 min or 120 min of cytokinin treatment (Fig. 1), confirming the results of the microarray analysis
Köllmer I, Werner T, Schmülling T - Ectopic expression of different cytokinin-regulated transcription factor genes of Arabidopsis thaliana alters plant growth and development
Visit the PubMed ArticleWe screened different collections for T-DNA insertional mutants of these four genes. One insertional mutant was identified for the GATA22 and one for the HAT4 gene. These were named gata22-1 and hat4-1, respectively, and homozygous lines were constructed for both of them. No transcripts for the respective genes were detected in either of the mutant lines
Köllmer I, Werner T, Schmülling T - Ectopic expression of different cytokinin-regulated transcription factor genes of Arabidopsis thaliana alters plant growth and development
Visit the PubMed Articlewe selected three genes that were at least two-fold upregulated after 15 min of cytokinin treatment (GATA22, bHLH64 and HAT22 induced 2.0-, 2.9- and 2.4-fold, respectively) and one more gene that was strongly induced after 120 min of cytokinin treatment (HAT4, 13.1-fold) for further analysis ... The transcript level of all chosen genes was enhanced about two- to four-fold after 15 min or 120 min of cytokinin treatment (Fig. 1), confirming the results of the microarray analysis
Köllmer I, Werner T, Schmülling T - Ectopic expression of different cytokinin-regulated transcription factor genes of Arabidopsis thaliana alters plant growth and development
Visit the PubMed ArticleConsistent with this idea of hormonal antagonism converging on GATA22 expression, it was found that GATA22 expression is induced by cytokinin but downregulated by gibberellin
Köllmer I, Werner T, Schmülling T - Ectopic expression of different cytokinin-regulated transcription factor genes of Arabidopsis thaliana alters plant growth and development
Visit the PubMed Articlewe selected three genes that were at least two-fold upregulated after 15 min of cytokinin treatment (GATA22, bHLH64 and HAT22 induced 2.0-, 2.9- and 2.4-fold, respectively) and one more gene that was strongly induced after 120 min of cytokinin treatment (HAT4, 13.1-fold) for further analysis ... The transcript level of all chosen genes was enhanced about two- to four-fold after 15 min or 120 min of cytokinin treatment (Fig. 1), confirming the results of the microarray analysis
Köllmer I, Werner T, Schmülling T - Ectopic expression of different cytokinin-regulated transcription factor genes of Arabidopsis thaliana alters plant growth and development
Visit the PubMed ArticleConsistent with this idea of hormonal antagonism converging on GATA22 expression, it was found that GATA22 expression is induced by cytokinin but downregulated by gibberellin
Köllmer I, Werner T, Schmülling T - Ectopic expression of different cytokinin-regulated transcription factor genes of Arabidopsis thaliana alters plant growth and development
Visit the PubMed ArticleWe screened different collections for T-DNA insertional mutants of these four genes. One insertional mutant was identified for the GATA22 and one for the HAT4 gene. These were named gata22-1 and hat4-1, respectively, and homozygous lines were constructed for both of them. No transcripts for the respective genes were detected in either of the mutant lines
Köllmer I, Werner T, Schmülling T - Ectopic expression of different cytokinin-regulated transcription factor genes of Arabidopsis thaliana alters plant growth and development
Visit the PubMed ArticleARL ... found to be ER-localized
Feng G, Qin Z, Yan J, Zhang X, Hu Y - Arabidopsis ORGAN SIZE RELATED1 regulates organ growth and final organ size in orchestration with ARGOS and ARL
Visit the PubMed Articlewe could not detect an obvious alteration of ... ARL mRNA levels in the osr1-1 mutant
Feng G, Qin Z, Yan J, Zhang X, Hu Y - Arabidopsis ORGAN SIZE RELATED1 regulates organ growth and final organ size in orchestration with ARGOS and ARL
Visit the PubMed Articleoverexpression of OSR1 resulted in suppression of ... ARL transcription
Feng G, Qin Z, Yan J, Zhang X, Hu Y - Arabidopsis ORGAN SIZE RELATED1 regulates organ growth and final organ size in orchestration with ARGOS and ARL
Visit the PubMed ArticleOSR1 appeared to contain a motif that is conserved in ARGOS and ARL (Fig. 1b). To further determine the tissue-specific expression of OSR1, we generated transgenic Arabidopsis plants carrying an OSR1 promoter::β-glucuronidase (proOSR1-GUS) fusion gene and examined GUS activities in transgenic plants. In 7-d-old seedlings ... low GUS expression was detected in cotyledons
Feng G, Qin Z, Yan J, Zhang X, Hu Y - Arabidopsis ORGAN SIZE RELATED1 regulates organ growth and final organ size in orchestration with ARGOS and ARL
Visit the PubMed ArticleAs the CycB1;1-GUS reporter marks the cells at the G2-M phase (Criqui et al., 2001), the enhanced GUS activities observed in young leaves and the root meristem region of 35S-OSR1 seedlings indicated the presence of more proliferating cells in the transgenic plants than in the control plants
Feng G, Qin Z, Yan J, Zhang X, Hu Y - Arabidopsis ORGAN SIZE RELATED1 regulates organ growth and final organ size in orchestration with ARGOS and ARL
Visit the PubMed Articleoverexpression of OSR1 resulted in suppression of ARGOS
Feng G, Qin Z, Yan J, Zhang X, Hu Y - Arabidopsis ORGAN SIZE RELATED1 regulates organ growth and final organ size in orchestration with ARGOS and ARL
Visit the PubMed ArticleOSR1 promoter::β-glucuronidase (proOSR1-GUS) fusion ... In expanding leaves, GUS staining was still detectable ... at the dispersed meristematic regions and the leaf margin
Feng G, Qin Z, Yan J, Zhang X, Hu Y - Arabidopsis ORGAN SIZE RELATED1 regulates organ growth and final organ size in orchestration with ARGOS and ARL
Visit the PubMed ArticleOSR1 is ... repressed by ... epi-brassinolide
Feng G, Qin Z, Yan J, Zhang X, Hu Y - Arabidopsis ORGAN SIZE RELATED1 regulates organ growth and final organ size in orchestration with ARGOS and ARL
Visit the PubMed ArticleOSR1 is ... repressed by ABA
Feng G, Qin Z, Yan J, Zhang X, Hu Y - Arabidopsis ORGAN SIZE RELATED1 regulates organ growth and final organ size in orchestration with ARGOS and ARL
Visit the PubMed ArticleOSR1 promoter::β-glucuronidase (proOSR1-GUS) fusion ... In expanding leaves, GUS staining was still detectable in the blade
Feng G, Qin Z, Yan J, Zhang X, Hu Y - Arabidopsis ORGAN SIZE RELATED1 regulates organ growth and final organ size in orchestration with ARGOS and ARL
Visit the PubMed ArticleTo further determine the tissue-specific expression of OSR1, we generated transgenic Arabidopsis plants carrying an OSR1 promoter::β-glucuronidase (proOSR1-GUS) fusion gene and examined GUS activities in transgenic plants. In 7-d-old seedlings, abundant GUS staining was detected in leaf primordia
Feng G, Qin Z, Yan J, Zhang X, Hu Y - Arabidopsis ORGAN SIZE RELATED1 regulates organ growth and final organ size in orchestration with ARGOS and ARL
Visit the PubMed ArticleWe then investigated whether the OSR1-GFP fusion protein co-localized with the ER marker protein Bip-RFP in protoplasts. As shown in Fig. 6(b), the green fluorescence signals of OSR1-GFP fusion proteins closely overlapped the red fluorescence signals of Bip-RFP, indicating that OSR1 is localized to the ER
Feng G, Qin Z, Yan J, Zhang X, Hu Y - Arabidopsis ORGAN SIZE RELATED1 regulates organ growth and final organ size in orchestration with ARGOS and ARL
Visit the PubMed Articlethe expression levels of ... CycD3;1 in expanding leaves were comparable between the two genotypes. However, persistent expression of ... CycD3;1 was detected even in leaves of 40-d-old 35S-OSR1 plants
Feng G, Qin Z, Yan J, Zhang X, Hu Y - Arabidopsis ORGAN SIZE RELATED1 regulates organ growth and final organ size in orchestration with ARGOS and ARL
Visit the PubMed ArticleOSR1 is induced by ethylene
Feng G, Qin Z, Yan J, Zhang X, Hu Y - Arabidopsis ORGAN SIZE RELATED1 regulates organ growth and final organ size in orchestration with ARGOS and ARL
Visit the PubMed ArticleOSR1 promoter::β-glucuronidase (proOSR1-GUS) fusion ... In expanding leaves, GUS staining was still detectable in ... the petiole
Feng G, Qin Z, Yan J, Zhang X, Hu Y - Arabidopsis ORGAN SIZE RELATED1 regulates organ growth and final organ size in orchestration with ARGOS and ARL
Visit the PubMed ArticleARGOS ... found to be ER-localized
Feng G, Qin Z, Yan J, Zhang X, Hu Y - Arabidopsis ORGAN SIZE RELATED1 regulates organ growth and final organ size in orchestration with ARGOS and ARL
Visit the PubMed Articlethe expression levels of ANT ... in expanding leaves were comparable between the two genotypes. However, persistent expression of ANT ... was detected even in leaves of 40-d-old 35S-OSR1 plants
Feng G, Qin Z, Yan J, Zhang X, Hu Y - Arabidopsis ORGAN SIZE RELATED1 regulates organ growth and final organ size in orchestration with ARGOS and ARL
Visit the PubMed Articleoverexpression of ARGOS also inhibited ARL expression
Feng G, Qin Z, Yan J, Zhang X, Hu Y - Arabidopsis ORGAN SIZE RELATED1 regulates organ growth and final organ size in orchestration with ARGOS and ARL
Visit the PubMed Articlewe could not detect an obvious alteration of ARGOS ... mRNA levels in the osr1-1 mutant
Feng G, Qin Z, Yan J, Zhang X, Hu Y - Arabidopsis ORGAN SIZE RELATED1 regulates organ growth and final organ size in orchestration with ARGOS and ARL
Visit the PubMed ArticleFurthermore, promKNAT7:GUS fusion expression is closely associated with the vascular system and interfascicular fibers in Arabidopsis seedlings, roots, and inflorescence stems
Li E, Wang S, Liu Y, Chen JG, Douglas CJ - OVATE FAMILY PROTEIN4 (OFP4) interaction with KNAT7 regulates secondary cell wall formation in Arabidopsis thaliana
Visit the PubMed Articlenuclear localization of OFP1
Li E, Wang S, Liu Y, Chen JG, Douglas CJ - OVATE FAMILY PROTEIN4 (OFP4) interaction with KNAT7 regulates secondary cell wall formation in Arabidopsis thaliana
Visit the PubMed ArticleIn 7-day-old seedlings (Figure 5d), promOFP4:GUS was expressed mainly in the root, at the root–hypocotyl junction, and in cotyledons ... in cotyledons, expression was observed in both veins and other tissues
Li E, Wang S, Liu Y, Chen JG, Douglas CJ - OVATE FAMILY PROTEIN4 (OFP4) interaction with KNAT7 regulates secondary cell wall formation in Arabidopsis thaliana
Visit the PubMed Articlewe transiently expressed ELA–green fluorescent protein fusions ... ELA1–GFP ... in onion epidermal cells ... microscopic analysis of the transformed onion cells revealed a similar fluorescence pattern between the two ELA–GFP ... and the m-gfp5-ER reporter known to be localized in the ER
Zhang Y, Zhang B, Yan D, Dong W, Yang W, Li Q, Zeng L, Wang J, Wang L, Hicks LM, He Z - Two Arabidopsis cytochrome P450 monooxygenases, CYP714A1 and CYP714A2, function redundantly in plant development through gibberellin deactivation
Visit the PubMed ArticleGA3ox1 was strongly upregulated in ELA1-OE
Zhang Y, Zhang B, Yan D, Dong W, Yang W, Li Q, Zeng L, Wang J, Wang L, Hicks LM, He Z - Two Arabidopsis cytochrome P450 monooxygenases, CYP714A1 and CYP714A2, function redundantly in plant development through gibberellin deactivation
Visit the PubMed ArticleELA2 was expressed in the leaf margin
Zhang Y, Zhang B, Yan D, Dong W, Yang W, Li Q, Zeng L, Wang J, Wang L, Hicks LM, He Z - Two Arabidopsis cytochrome P450 monooxygenases, CYP714A1 and CYP714A2, function redundantly in plant development through gibberellin deactivation
Visit the PubMed ArticleGA3ox1 was strongly upregulated in ... ELA2-OE
Zhang Y, Zhang B, Yan D, Dong W, Yang W, Li Q, Zeng L, Wang J, Wang L, Hicks LM, He Z - Two Arabidopsis cytochrome P450 monooxygenases, CYP714A1 and CYP714A2, function redundantly in plant development through gibberellin deactivation
Visit the PubMed ArticleELA2 was expressed in the ... petiole vein of cotyledons
Zhang Y, Zhang B, Yan D, Dong W, Yang W, Li Q, Zeng L, Wang J, Wang L, Hicks LM, He Z - Two Arabidopsis cytochrome P450 monooxygenases, CYP714A1 and CYP714A2, function redundantly in plant development through gibberellin deactivation
Visit the PubMed ArticleGIDc ... also slightly upregulated in ELA1-OE
Zhang Y, Zhang B, Yan D, Dong W, Yang W, Li Q, Zeng L, Wang J, Wang L, Hicks LM, He Z - Two Arabidopsis cytochrome P450 monooxygenases, CYP714A1 and CYP714A2, function redundantly in plant development through gibberellin deactivation
Visit the PubMed ArticleELA2–GUS was expressed in the ... petioles of young leaves
Zhang Y, Zhang B, Yan D, Dong W, Yang W, Li Q, Zeng L, Wang J, Wang L, Hicks LM, He Z - Two Arabidopsis cytochrome P450 monooxygenases, CYP714A1 and CYP714A2, function redundantly in plant development through gibberellin deactivation
Visit the PubMed ArticleWe also generated a fusion protein between ELA2 and the red fluorescent protein of Discosoma sp. reef coral (DsRED), co-transformed ELA2–DsRED or DsRED with the m-gfp5-ER protein into the onion epidermal cells, and examined their localization patterns. As shown in Figures (2d–i), while there was little overlap between DsRED and gfp5-ER, ELA2–DsRED was found to be co-localized with the m-gfp5-ER
Zhang Y, Zhang B, Yan D, Dong W, Yang W, Li Q, Zeng L, Wang J, Wang L, Hicks LM, He Z - Two Arabidopsis cytochrome P450 monooxygenases, CYP714A1 and CYP714A2, function redundantly in plant development through gibberellin deactivation
Visit the PubMed ArticleELA2–GUS was expressed in the SAM
Zhang Y, Zhang B, Yan D, Dong W, Yang W, Li Q, Zeng L, Wang J, Wang L, Hicks LM, He Z - Two Arabidopsis cytochrome P450 monooxygenases, CYP714A1 and CYP714A2, function redundantly in plant development through gibberellin deactivation
Visit the PubMed ArticleReverse-transcription PCR (RT-PCR) analysis of ELA1 or ELA2 transcripts confirmed that ELA1 and ELA2 exhibit partially overlapping expression patterns
Zhang Y, Zhang B, Yan D, Dong W, Yang W, Li Q, Zeng L, Wang J, Wang L, Hicks LM, He Z - Two Arabidopsis cytochrome P450 monooxygenases, CYP714A1 and CYP714A2, function redundantly in plant development through gibberellin deactivation
Visit the PubMed ArticleELA1–GUS expression was observed in the shoot apical meristem
Zhang Y, Zhang B, Yan D, Dong W, Yang W, Li Q, Zeng L, Wang J, Wang L, Hicks LM, He Z - Two Arabidopsis cytochrome P450 monooxygenases, CYP714A1 and CYP714A2, function redundantly in plant development through gibberellin deactivation
Visit the PubMed ArticleELA1–GUS expression was observed in the ... petioles of young leaves and emerging leaves
Zhang Y, Zhang B, Yan D, Dong W, Yang W, Li Q, Zeng L, Wang J, Wang L, Hicks LM, He Z - Two Arabidopsis cytochrome P450 monooxygenases, CYP714A1 and CYP714A2, function redundantly in plant development through gibberellin deactivation
Visit the PubMed Articlewe transiently expressed ELA–green fluorescent protein fusions ... ELA2–GFP ... in onion epidermal cells ... microscopic analysis of the transformed onion cells revealed a similar fluorescence pattern between the two ELA–GFP ... and the m-gfp5-ER reporter known to be localized in the ER
Zhang Y, Zhang B, Yan D, Dong W, Yang W, Li Q, Zeng L, Wang J, Wang L, Hicks LM, He Z - Two Arabidopsis cytochrome P450 monooxygenases, CYP714A1 and CYP714A2, function redundantly in plant development through gibberellin deactivation
Visit the PubMed ArticleWe also observed that GA2ox2 was upregulated in ELA2-OE plants
Zhang Y, Zhang B, Yan D, Dong W, Yang W, Li Q, Zeng L, Wang J, Wang L, Hicks LM, He Z - Two Arabidopsis cytochrome P450 monooxygenases, CYP714A1 and CYP714A2, function redundantly in plant development through gibberellin deactivation
Visit the PubMed ArticleReverse-transcription PCR (RT-PCR) analysis of ELA1 or ELA2 transcripts confirmed that ELA1 and ELA2 exhibit partially overlapping expression patterns
Zhang Y, Zhang B, Yan D, Dong W, Yang W, Li Q, Zeng L, Wang J, Wang L, Hicks LM, He Z - Two Arabidopsis cytochrome P450 monooxygenases, CYP714A1 and CYP714A2, function redundantly in plant development through gibberellin deactivation
Visit the PubMed ArticleGID1a ... also slightly upregulated in ELA1-OE
Zhang Y, Zhang B, Yan D, Dong W, Yang W, Li Q, Zeng L, Wang J, Wang L, Hicks LM, He Z - Two Arabidopsis cytochrome P450 monooxygenases, CYP714A1 and CYP714A2, function redundantly in plant development through gibberellin deactivation
Visit the PubMed Articlepnp1 ... The introns discussed here belong to Group II, whose splicing mechanism entails lariat formation (reviewed in Stern et al., 2010). The amount of the lariat junction can be measured by qRT-PCR using two primers pointing towards the junction, and Figure 4(b) shows results for the amount of lariat junction-containing petD intron fragments in the WT and null mutants. While there was some variability, each mutant accumulates four to eightfold more lariat junctions than the WT. This suggests that the pnp-specific intron-containing bands 5 and 6 in Figure 3(b) include the lariat junction, and because they are shorter than the full-length excised intron (band 4), represent intron degradation intermediates. We subsequently decided to focus on band 5, the most abundant novel intron-containing band in the null mutants. To map its ends we used cRT-PCR. As shown in the Figure 5 inset, samples not treated with T4 RNA ligase gave rise to amplification of the intact intron (open arrowhead), as expected. However, ligated samples generated additional bands (full arrowheads), with a major pnp1-1 product absent from the WT sample, suggesting that it might represent the gel band of interest from Figure 3(b). A faint and smaller band was amplified in the WT. Both products were cloned and sequenced, and although their ends were variable (see below), the WT and pnp1-1 products lacked approximately 240 and 200 nt, respectively, in comparison with the 709-nt full-length intron
Germain A, Herlich S, Larom S, Kim SH, Schuster G, Stern DB - Mutational analysis of Arabidopsis chloroplast polynucleotide phosphorylase reveals roles for both RNase PH core domains in polyadenylation, RNA 3'-end maturation and intron degradation
Visit the PubMed Articlepnp1 ... We also confirmed (Figure S2a) previously published 3′ extensions for the monocistronic rbcL and psbA transcripts (Marchive et al., 2009), which in the case of rbcL was verified by gel blot (Figure S2b). Again, a mixture of the WT and null mutant pattern was observed for P184L. In addition, we examined the dicistronic atpB-atpE gene cluster (Figure S2c), whose slightly more complex transcript pattern derives from the presence of two promoters as well as processing to yield atpE-specific species (Schweer et al., 2006). Again, the pattern was interpretable as 3′-end extensions although in this case, P184L appeared as the WT. This finding suggests that atpBE 3′-end maturation is less sensitive to the amount of cpPNPase activity
Germain A, Herlich S, Larom S, Kim SH, Schuster G, Stern DB - Mutational analysis of Arabidopsis chloroplast polynucleotide phosphorylase reveals roles for both RNase PH core domains in polyadenylation, RNA 3'-end maturation and intron degradation
Visit the PubMed Articlepnp1 ... Also of note were four novel bands observed for atpH (marked 2–5). Bands 2 and 5 were present only in the null mutants, band 3 was prominent in the null mutants and P184L, and present weakly in another mutant of the first core domain, S202N. Band 4 was present in all mutants, but most strongly in the weak allele P184L. One possible interpretation could be that bands 2–5 represent the products of a succession of endonucleolytic cleavages between atpH and atpF that are subsequently trimmed by PNPase. This situation would be consistent with a recently proposed model for chloroplast polycistronic RNA processing (Pfalz et al., 2009). Their reduced intensities in the weak alleles probably reflect the presence of residual cpPNPase activity in those mutants. In the case of band 4, however, the residual activity increases its abundance perhaps, for example, by trimming band 2 or 3. The precise 3′-ends of atpH transcripts were compared between the WT and pnp1-1 using circular RT-PCR (cRT-PCR; Figure 2c). WT transcripts had two main 3′-ends. The end nearest the coding region was well represented in pnp1-1, suggesting it might be generated by transcription termination. The pnp1-1 mutant also accumulated a series of extended 3′-ends, as suggested by the gel blot, and in keeping with the possibility that they are endonucleolytic cleavage products
Germain A, Herlich S, Larom S, Kim SH, Schuster G, Stern DB - Mutational analysis of Arabidopsis chloroplast polynucleotide phosphorylase reveals roles for both RNase PH core domains in polyadenylation, RNA 3'-end maturation and intron degradation
Visit the PubMed ArticleTo extend this analysis, we investigated the composition of poly(A) tails in the WT and pnp1-1 using oligo(dT)-primed RT-PCR. As shown in Table 1, both homo- and heteropolymeric tails were found for the psbA mRNA in the WT, while only homopolymeric A-tails were present in pnp1-1
Germain A, Herlich S, Larom S, Kim SH, Schuster G, Stern DB - Mutational analysis of Arabidopsis chloroplast polynucleotide phosphorylase reveals roles for both RNase PH core domains in polyadenylation, RNA 3'-end maturation and intron degradation
Visit the PubMed Articlepnp1 ... Figure 5 shows a model of the petD intron upon which the 5′- and 3′-ends of cRT-PCR products are displayed. The cloned 5′-ends were similar in the WT and pnp1-1, with most being clustered in a 6-nt stretch in the first stem of Domain I, and most of the remaining ones in the loop of Domain II. These 5′-ends may represent sites of endonucleolytic cleavage, and would be expected to be impervious to PNPase activity, which has strict 3′→5′ polarity. In contrast, the WT and mutant had distinct clusters of 3′-ends, both located in Domain I. These were in the exon binding domain 2 (EBS2) for pnp1-1, and in a Domain I stem for the WT. These two clusters are separated by approximately 40 nt, which is consistent with the size difference of the cloned PCR products. The simplest interpretation is that PNPase normally completes degradation of the linearized intron, while occasionally stalling at the location marked by 3′-ends in the WT. On the other hand, PNPase seems to be required to degrade the intron sequences starting at EBS2
Germain A, Herlich S, Larom S, Kim SH, Schuster G, Stern DB - Mutational analysis of Arabidopsis chloroplast polynucleotide phosphorylase reveals roles for both RNase PH core domains in polyadenylation, RNA 3'-end maturation and intron degradation
Visit the PubMed Articlepnp1 ... In the psbB gene cluster (Figure 3), two genes (petB and petD) contain introns, while psbN is expressed from its own promoter on the opposite strand. As shown in Figure 3(b), the various coding region probes, with the exception of psbN, revealed patterns that while more complex, were reminiscent of other genes described above, with the null mutants showing stronger effects, and P184L and S202N intermediate phenotypes. On the other hand, transcripts appeared to be less diffuse than in the case of the atpI cluster (Figure 2), suggesting that their 3′-ends might be more homogeneous. We checked this by cRT-PCR, using petD as an example (Figure 3c), and found that although it had multiple 3′-ends, they were indeed less dispersed than for atpH
Germain A, Herlich S, Larom S, Kim SH, Schuster G, Stern DB - Mutational analysis of Arabidopsis chloroplast polynucleotide phosphorylase reveals roles for both RNase PH core domains in polyadenylation, RNA 3'-end maturation and intron degradation
Visit the PubMed Articlepnp1 ... In the case of psbN, the null mutants were nearly depleted for the transcript (Figure 3b) which, however, is dispensable for normal PSII function
Germain A, Herlich S, Larom S, Kim SH, Schuster G, Stern DB - Mutational analysis of Arabidopsis chloroplast polynucleotide phosphorylase reveals roles for both RNase PH core domains in polyadenylation, RNA 3'-end maturation and intron degradation
Visit the PubMed Articlepnp1 ... We analyzed two such clusters, namely those encoding atpI/atpH/atpF/atpA and psbB/psbT/psbN/psbH/petB/petD. For the atpI gene cluster (Figure 2), a molecular phenotype common to null mutants for all five probes used is the presence of non-discrete high molecular transcripts that correlate with the diminution of most of the corresponding discrete bands (Figure 2b). These may represent imprecise 3′-end formation rather than transcript instability
Germain A, Herlich S, Larom S, Kim SH, Schuster G, Stern DB - Mutational analysis of Arabidopsis chloroplast polynucleotide phosphorylase reveals roles for both RNase PH core domains in polyadenylation, RNA 3'-end maturation and intron degradation
Visit the PubMed ArticleqRT-PCR analysis of the atpI coding region showed no difference between the WT and pnp1-1 (data not shown), although only the band marked ‘1’ remains discrete and unchanged
Germain A, Herlich S, Larom S, Kim SH, Schuster G, Stern DB - Mutational analysis of Arabidopsis chloroplast polynucleotide phosphorylase reveals roles for both RNase PH core domains in polyadenylation, RNA 3'-end maturation and intron degradation
Visit the PubMed ArticleTILLING mutants, D625N appeared to overaccumulate PNPase, P184L was comparable to the WT, S202N slightly lower than the WT, and for G596R, no signal could be detected in either native or denaturing gels
Germain A, Herlich S, Larom S, Kim SH, Schuster G, Stern DB - Mutational analysis of Arabidopsis chloroplast polynucleotide phosphorylase reveals roles for both RNase PH core domains in polyadenylation, RNA 3'-end maturation and intron degradation
Visit the PubMed Articlepnp1 ... for psbB a band shorter than any WT transcript was observed (band 1), which we speculate is a degradation intermediate
Germain A, Herlich S, Larom S, Kim SH, Schuster G, Stern DB - Mutational analysis of Arabidopsis chloroplast polynucleotide phosphorylase reveals roles for both RNase PH core domains in polyadenylation, RNA 3'-end maturation and intron degradation
Visit the PubMed Articlepnp1 ... Also of note was the fact that while the psbA 3′-end tails were longer in the WT compared with the mutant, 60% of the tails present in WT were heterogeneous at their 5′-ends, but terminated in 49–150-nt stretches of adenosines, suggesting that they may have been initiated by PNPase activity and elongated by PAP. The three 23S rRNA tails found from the WT were homogeneous poly(A), as were most of the tails from 16S rRNA. This contrasted with the rather heterogeneous tails of the psbA mRNA
Germain A, Herlich S, Larom S, Kim SH, Schuster G, Stern DB - Mutational analysis of Arabidopsis chloroplast polynucleotide phosphorylase reveals roles for both RNase PH core domains in polyadenylation, RNA 3'-end maturation and intron degradation
Visit the PubMed Articletotal RNA was first examined by gel electrophoresis and ethidium bromide staining, revealing a reduced mobility for one of the fragments comprising chloroplast 23S rRNA for the three null mutants, as well as G596R and D625N (Figure S1). This is due to defective exonucleolytic trimming of approximately 100 nucleotides (nt) of the intergenic region between 23S and 4.5S rRNA
Germain A, Herlich S, Larom S, Kim SH, Schuster G, Stern DB - Mutational analysis of Arabidopsis chloroplast polynucleotide phosphorylase reveals roles for both RNase PH core domains in polyadenylation, RNA 3'-end maturation and intron degradation
Visit the PubMed Articlepnp1 ... We were particularly intrigued by the hybridization patterns for the petB and petD introns (Figure 3b), in which bands shorter than the complete excised introns (bands 2 and 4) were detected, these being band 3 for the petB intron and bands 5 and 6 for the petD intron
Germain A, Herlich S, Larom S, Kim SH, Schuster G, Stern DB - Mutational analysis of Arabidopsis chloroplast polynucleotide phosphorylase reveals roles for both RNase PH core domains in polyadenylation, RNA 3'-end maturation and intron degradation
Visit the PubMed ArticleAn intermediate phenotype was observed for P184L, in which a conserved residue located in the phosphorolytically inactive first core domain is altered. In this case, a mixture of mature and 3′-extended 23S rRNA was observed. Thus, with respect to 23S rRNA maturation, P184L is a weak pnp mutant allele; the other mutants appeared as the WT
Germain A, Herlich S, Larom S, Kim SH, Schuster G, Stern DB - Mutational analysis of Arabidopsis chloroplast polynucleotide phosphorylase reveals roles for both RNase PH core domains in polyadenylation, RNA 3'-end maturation and intron degradation
Visit the PubMed ArticleCKX3 is normally expressed ... not in leaf primordia
Holst K, Schmülling T, Werner T - Enhanced cytokinin degradation in leaf primordia of transgenic Arabidopsis plants reduces leaf size and shoot organ primordia formation
Visit the PubMed ArticleCKX3 is normally expressed in the central zone of the meristem ([Gordon et al., 2009] and [Bartrina et al., 2011
Holst K, Schmülling T, Werner T - Enhanced cytokinin degradation in leaf primordia of transgenic Arabidopsis plants reduces leaf size and shoot organ primordia formation
Visit the PubMed ArticleLOX2 transcript level ... was significantly reduced in tcp4-1 plants
Sarvepalli K, Nath U - Hyper-activation of the TCP4 transcription factor in Arabidopsis thaliana accelerates multiple aspects of plant maturation
Visit the PubMed ArticleLOX2 transcript level ... was not significantly up-regulated in TCP4:VP16-C plants compared to Col-0
Sarvepalli K, Nath U - Hyper-activation of the TCP4 transcription factor in Arabidopsis thaliana accelerates multiple aspects of plant maturation
Visit the PubMed ArticleSeveral AGG3pro:GUS reporter lines were produced but displayed aberrantly high expression in most tissues assayed
Chakravorty D, Trusov Y, Zhang W, Acharya BR, Sheahan MB, McCurdy DW, Assmann SM, Botella JR. - An atypical heterotrimeric G-protein ?-subunit is involved in guard cell K?-channel regulation and morphological development in Arabidopsis thaliana
Visit the PubMed ArticleNorthern blot analysis showed that AGG3 expression was highest in reproductive tissues, with comparatively low expression observed in all other tissues analysed
Chakravorty D, Trusov Y, Zhang W, Acharya BR, Sheahan MB, McCurdy DW, Assmann SM, Botella JR. - An atypical heterotrimeric G-protein ?-subunit is involved in guard cell K?-channel regulation and morphological development in Arabidopsis thaliana
Visit the PubMed ArticleBoth GFP–AGG3 and AGG3–GFP showed fluorescence clearly limited to the periphery of the cell in roots and leaves of stably transformed Arabidopsis lines (Figure 5a–c), although they also showed small motile vesicle-like spots (Figure 5c), much like the Golgi-localization reported for AGG1, but seldom seen for AGG2 ... When cells from GFP fusion transgenic lines were partially plasmolysed the fluorescence shrank away from the cell walls and was confined to the periphery of the protoplast (Figure 5d,e), demonstrating that AGG3 is located on the plasma membrane, not the extracellular space
Chakravorty D, Trusov Y, Zhang W, Acharya BR, Sheahan MB, McCurdy DW, Assmann SM, Botella JR. - An atypical heterotrimeric G-protein ?-subunit is involved in guard cell K?-channel regulation and morphological development in Arabidopsis thaliana
Visit the PubMed ArticleMembrane localization was also observed when tobacco leaves were infiltrated with the GFP fusion constructs (Figure 5f–h). The intensity of the GFP signal almost doubled when tobacco leaves were co-infiltrated with a 35Spro:AGB1 construct (compare Figure 5g with 5h and quantified in 5i), indicating that the presence of an additional β-subunit helps stabilize AGG3, as observed for other Arabidopsis γ-subunits (Adjobo-Hermans et al., 2006; Zeng et al., 2007). However, unlike AGG1, localization of AGG3 in the motile vesicular-like spots appeared to increase when co-infiltrated with AGB1. Such an increase may indicate retention of, and therefore possibly a function for, AGB1/AGG3 dimers on or within internal vesicles
Chakravorty D, Trusov Y, Zhang W, Acharya BR, Sheahan MB, McCurdy DW, Assmann SM, Botella JR. - An atypical heterotrimeric G-protein ?-subunit is involved in guard cell K?-channel regulation and morphological development in Arabidopsis thaliana
Visit the PubMed ArticlePerformance of RT-PCR on guard cell mRNA confirmed that AGG3 is expressed in these cells
Chakravorty D, Trusov Y, Zhang W, Acharya BR, Sheahan MB, McCurdy DW, Assmann SM, Botella JR. - An atypical heterotrimeric G-protein ?-subunit is involved in guard cell K?-channel regulation and morphological development in Arabidopsis thaliana
Visit the PubMed ArticleAs leaves mature, TCP14 ... 15 ... gene expression in leaves is repressed in a apical to basal (basiplastic) manner
Kieffer M, Master V, Waites R, Davies B - TCP14 and TCP15 affect internode length and leaf shape in Arabidopsis
Visit the PubMed ArticleTCP14 is expressed ... in ... developing leaf vascular bundles
Kieffer M, Master V, Waites R, Davies B - TCP14 and TCP15 affect internode length and leaf shape in Arabidopsis
Visit the PubMed ArticleTCP14 is also expressed at low levels in the vegetative shoot apical meristem
Kieffer M, Master V, Waites R, Davies B - TCP14 and TCP15 affect internode length and leaf shape in Arabidopsis
Visit the PubMed ArticleTCP14 ... expressed in leaf primordia ... TCP14 is expressed more widely in the leaf blade (Figure 3E,G), including young trichomes (Figure 3D) and developing leaf vascular bundles
Kieffer M, Master V, Waites R, Davies B - TCP14 and TCP15 affect internode length and leaf shape in Arabidopsis
Visit the PubMed ArticleTCP14 is expressed at the base of the cotyledons
Kieffer M, Master V, Waites R, Davies B - TCP14 and TCP15 affect internode length and leaf shape in Arabidopsis
Visit the PubMed ArticleTCP15 ... expressed in leaf primordia ... TCP15 is mostly expressed in primordial leaf margins
Kieffer M, Master V, Waites R, Davies B - TCP14 and TCP15 affect internode length and leaf shape in Arabidopsis
Visit the PubMed ArticleTCP14 is expressed more widely in the leaf blade ... As leaves mature, TCP14/15 gene expression in leaves is repressed in a apical to basal (basiplastic) manner ... As leaves mature, TCP14/15 gene expression in leaves is repressed in a apical to basal (basiplastic) manner
Kieffer M, Master V, Waites R, Davies B - TCP14 and TCP15 affect internode length and leaf shape in Arabidopsis
Visit the PubMed ArticleTCP14 is expressed ... in ... young trichomes
Kieffer M, Master V, Waites R, Davies B - TCP14 and TCP15 affect internode length and leaf shape in Arabidopsis
Visit the PubMed ArticleNo signal corresponding to the 5′ part of the ETS was detected in ... rrp6l2
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed Articlefragments of approximately 0.4–1.7 kb, representing degradation intermediates of the 5′ ETS, accumulated ... to higher levels in xrn2 xrn3
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed Articlewe detected MTR4 promoter activity in ... apical ... meristems
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed Articlesignificant MTR4 promoter activity was observed in vascular tissues of ... leaves
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed Articlesignificant MTR4 promoter activity was observed in vascular tissues of cotyledons
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed ArticleProbe S2, situated in the 3′ part of the 5′ ETS, detected ... 35S(P) and P–A3 intermediates in ... xrn4 ... small fragment of approximately 200 nt was detected
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed ArticleNo signal corresponding to the 5′ part of the ETS was detected in ... mtr4
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed ArticleOur data show that mtr4 mutants accumulate the 5′ ETS downstream of site P as well as 5.8S and 18S rRNAs precursors, but show no obvious defects in 25S or 5S processing and accumulate wild-type steady-state levels of the four rRNAs
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed ArticleProbe S2, situated in the 3′ part of the 5′ ETS, detected 35S* precursors and 5′ ETS–A3 fragments in ... fry1 mutants ... In addition, signals of approximately 1.4–1.7 kb were seen for xrn2 mutants, and were more pronounced in ... fry1
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed ArticleProbe S2, situated in the 3′ part of the 5′ ETS, detected 35S* precursors and 5′ ETS–A3 fragments in xrn2 ... In addition, signals of approximately 1.4–1.7 kb were seen for xrn2 mutants
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed ArticleNo signal corresponding to the 5′ part of the ETS was detected in ... xrn4
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed ArticleTo test whether RRP6L1 or RRP6L2 contribute to processing of 5.8S rRNA, we analysed total RNA from wild-type, mtr4, rrp6l1, rrp6l2 and rrp6l1 rrp6l2 plants. All plants had similar levels of mature 5.8S rRNA. 5.8S rRNA processing intermediates were detected in rrp6l2 but not rrp6l1 mutants, although rrp6l2 plants accumulated fewer 5.8S rRNA precursors than mtr4 mutants
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed Articlesimultaneous down-regulation of both AtMTR4 and RRP6L2 resulted in both strong accumulation of 5.8S precursors and mild reduction of mature 5.8S rRNAs
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed ArticleSmall RNA blots were hybridized to a series of probes whose locations are indicated in Figure 7(a). Hybridization with probe S1 detected small RNAs in xrn2 xrn3 ... while probe S2 detected small RNAs in xrn2 xrn3
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed ArticleThe S1 probe detected 35S* precursor transcripts comprising the entire 5′ ETS, all three rRNAs and the 3′ ETS in ... fry1 mutants
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed ArticleNo signal corresponding to the 5′ part of the ETS was detected in ... rrp6l1
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed ArticleTo test whether RRP6L1 or RRP6L2 contribute to processing of 5.8S rRNA, we analysed total RNA from wild-type, mtr4, rrp6l1, rrp6l2 and rrp6l1 rrp6l2 plants. All plants had similar levels of mature 5.8S rRNA. 5.8S rRNA processing intermediates were detected in rrp6l2 but not rrp6l1 mutants, although rrp6l2 plants accumulated fewer 5.8S rRNA precursors than mtr4 mutants
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed ArticleProbe S2, situated in the 3′ part of the 5′ ETS, detected ... 35S(P) and P–A3 intermediates in ... rrp6l2 ... probe S2 revealed a strong signal of 500 nt in mtr4 mutants and to a lesser extent in rrp6l2 plants ... Finally, a small fragment of approximately 200 nt was detected
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed ArticleThe S1 probe detected 35S* precursor transcripts comprising the entire 5′ ETS, all three rRNAs and the 3′ ETS in ... xrn2 xrn3 ... mutants
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed ArticleThe size of these small RNAs is 21 nt, as determined by comparison with known 24 nt siRNAs generated from the promoter region (Figure 7c) (Preuss et al., 2008). These small RNAs were also detected by a probe from P1 to P′, but not when we used a probe spanning P to P1 (Figure 7d). A radiolabelled DNA probe spanning 100 nt of mature 5.8S rRNA and 150 nt of the ITS2 detected 21 nt small RNAs in mtr4, but not in any of the other samples (Figure 7b). These result shows that, when default degradation pathways are disrupted, accumulating rRNA processing intermediates and non-functional maturation by-products can become substrates of pathway that generates small RNAs
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed ArticleWe detected three 5.8S processing intermediates that accumulated strongly in mtr4 samples as compared to wild-type (Figure 3b,c). The relative levels of the precursors varied between samples (Figure S6b), but accumulated in all samples compared to wild-type. None of the three intermediates was detected using a probe situated just 5′ of mature 5.8S rRNA, indicating that all molecules had correctly processed 5′ ends (data not shown). However, steady-state levels of mature 5.8S rRNA were identical in wild-type and mtr4 plants (Figure 3b). To identify the 5.8S rRNA precursors observed in mtr4 plants, we performed circular RT-PCR. After RNA ligation, cDNA synthesis was initiated using a reverse primer situated inside mature 5.8S rRNA. PCR products spanning the ligated 5′ and 3′ ends were amplified using a reverse primer situated inside mature 5.8S rRNA and a forward primer situated immediately 3′ to mature 5.8S rRNA. All clones had correctly processed 5′ ends, but the 3′ ends mapped between positions 4084 and 4089 (wild-type 11/18, mtr4 10/27) or between positions 4191 and 4194 (wild-type 6/18, mtr4 13/27) from the transcription start site. A few clones (wild-type 1/18, mtr4 4/27) map to positions 4118 and 4142, respectively. These results indicate that the smallest pre-5.8S species observed on the Northern blot harbours a short 3′ extension of 10 nt, while the largest 5.8S precursor contains 120 nt of ITS2 (Figure 3). These results show that AtMTR4, unlike its yeast counterpart, is not essential for 3′ maturation of 5.8S rRNA
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed Articlefragments of approximately 0.4–1.7 kb, representing degradation intermediates of the 5′ ETS, accumulated in xrn2 plants
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed ArticleThe levels of large rRNA precursors comprising both 5.8S and 25S rRNAs were almost similar in mtr4 and wild-type plants, but mtr4 mutants accumulated higher amounts of 3′ extended 5.8S molecules in the small molecular weight range
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed Articlewe detected MTR4 promoter activity in all organs. Strong activity was detected in embryos
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed ArticleProbe S2, situated in the 3′ part of the 5′ ETS, detected ... 35S(P) and P–A3 intermediates in ... xrn3 ... a small fragment of approximately 200 nt was detected
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed ArticleSmall RNA blots were hybridized to a series of probes whose locations are indicated in Figure 7(a). Hybridization with probe S1 detected small RNAs in ... fry1 samples, while probe S2 detected small RNAs in ... fry1
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed ArticleProbe S2, situated in the 3′ part of the 5′ ETS, detected 35S* precursors and 5′ ETS–A3 fragments in ... xrn2 xrn3 ... In addition, signals of approximately 1.4–1.7 kb were seen for xrn2 mutants, and were more pronounced in xrn2 xrn3
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed ArticleSmall RNA blots were hybridized to a series of probes whose locations are indicated in Figure 7 ... probe S2 detected small RNAs in ... mtr4 samples
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed ArticleWe next examined 18S rRNA processing in mtr4 plants. Levels of fully processed 18S rRNA were similar in all plants, indicating that AtMTR4 is not required for 18S rRNA maturation (Figure 5a, probe S6). Probes situated 5′ or 3′ to mature 18S rRNA detected the 35S(P) rRNA precursor in both wild-type and mtr4 plants, and two smaller processing intermediates that accumulated to different levels (Figure 5a, probes S5 and S7). A fragment of 2.6 kb was present in wild-type but almost absent in mtr4 samples. This 18S precursor was also detected using a probe in the 3′ part of the 5′ ETS (Figure 6b), and corresponds to the P–A3 intermediate generated by cleavage of the 35S(P) precursor at position 3835 (Zakrzewska-Placzek et al., 2010). A second signal of 2 kb was stronger in mtr4 than in wild-type (Figure 5a, probes S5 and S7). To map the 3′ and 5′ ends of these processing intermediates, we performed circular RT-PCR. When we used oligonucleotides situated 3′ of the A2 site (o4) (Zakrzewska-Placzek et al., 2010) and 5′ of mature 18S (o1), all 3′ extremities corresponded to site A3, whereas the 5′ extremities matched either the P site or the P′ site (Figure 5c). The P′ site is situated 80 nt upstream of the 18S rRNA (Lange et al., 2008). Therefore, the 2.6 and 2 kb 18S rRNA precursors correspond to P–A3 and P′–A3, respectively. The Northern analysis shows that P–A3 is mostly detected in wild-type while P′–A3 is the major form that accumulates in mtr4 (Figure 5a, probes S5 and S7). Next, we used oligonucleotides situated inside mature 18S (o2) and immediately 3′ of mature 18S rRNA (o3) (Figure 5c, right panel). All sequences obtained from wild-type samples had mature 5′ ends. The 3′ ends mapped either to the A3 site (10/25) or to positions 3662–3667 (15/25), which are close to a second endonucleolytic cleavage site like the S. cerevisiae A2 site (Zakrzewska-Placzek et al., 2010). Both fragments were also amplified from mtr4 samples, albeit with a different ratio: the majority of clones (20/34) matched the 5′ 18S–A3 fragment, and only 8/34 clones matched the shorter 5′ 18S–A2 fragment. Six of the 34 clones obtained from mtr4 samples corresponded to the P′-A3 fragment. Non-encoded tails of 5–20 adenosines were present on 25 of the 28 clones corresponding to the P′-A3 fragment, and 25 of the 30 clones corresponding to the 5′ 18S-A3 fragment obtained from wild-type or mtr4 (Figure 5c). By contrast, only four of the 23 5′ 18S–A2 fragments were polyadenylated. These results show that four pre-18S species – P–A3, P′–A3, 5′ 18S–A3 and 5′ 18S–A2 – are produced in both wild-type and mtr4 plants. Cleavage of the P–A3 precursor at the P′ site produces the P′–A3 intermediate as a common rRNA processing intermediate. Its polyadenylation status and the accumulation of this fragment in mtr4 mutants suggests that the P′–A3 intermediate is a substrate of AtMTR4 and a polyadenylation-assisted 3′→5′ exoribonuclease. Clearly, the absence of AtMTR4 does not affect processing at the 5′ end of 18S rRNA. However, compared to wild-type, mtr4 plants accumulate more 5′ 18S–A3 fragments than 5′ 18S–A2 fragments, suggesting that 3′ processing of pre-18S species may be delayed
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed ArticleProbe S2, situated in the 3′ part of the 5′ ETS, detected ... 35S(P) and P–A3 intermediates in ... mtr4 ... probe S2 revealed a strong signal of 500 nt in mtr4 mutants ... The 3′ and 5′ ends of this fragment were identified by circular RT-PCR and mapped exactly to positions 1275 (site P) and 1757 (site P′), respectively. Hence, this fragment is generated by cleavage at P and P′. Accumulation of the P–P′ fragment in mtr4 plants shows that AtMTR4 is required for efficient degradation of this rRNA maturation by-product. Finally, a small fragment of approximately 200 nt was detected
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed ArticleTo test whether RRP6L1 or RRP6L2 contribute to processing of 5.8S rRNA, we analysed total RNA from wild-type, mtr4, rrp6l1, rrp6l2 and rrp6l1 rrp6l2 plants. All plants had similar levels of mature 5.8S rRNA. 5.8S rRNA processing intermediates were detected in rrp6l2 but not rrp6l1 mutants, although rrp6l2 plants accumulated fewer 5.8S rRNA precursors than mtr4 mutants
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed ArticleNo signal corresponding to the 5′ part of the ETS was detected in wild-type, xrn3
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed ArticleProbe S2, situated in the 3′ part of the 5′ ETS, detected ... 35S(P) and P–A3 intermediates in ... rrp6l1 ... Finally, a small fragment of approximately 200 nt was detected
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed Articlethe accumulation of both 5.8S rRNA precursors and P–P′ fragments was similar in mtr4 dcl2 dcl4 and mtr4 plants (Figure 7g), indicating that small RNA pathways probably do not contribute to removal of incompletely processed precursors or maturation by-products
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed ArticleWe then compared the rate of 5.8S rRNA synthesis between wild-type and mtr4 plants. 5.8S rRNA was chosen as mtr4 mutants accumulate 5.8S rRNA precursors. In addition, the size of the 5.8S rRNA makes it easily to compare with 5S rRNA, both of which are incorporated in equimolar amounts in ribosomes but are transcribed from distinct loci. We followed rRNA production in vivo by incorporation of radiolabelled UTP in de novo synthesized 5S and 5.8S rRNA over periods of 4, 6 and 8 h. Uptake of the radiolabelled nucleotide was similar in wild-type and mtr4 seedlings as estimated by scintillation counting of crude extracts. Analysis of low-molecular-weight RNAs by gel electrophoresis showed that, although steady-state levels of mature rRNAs were similar in wild-type and mtr4 seedlings, incorporation of the radiolabel in both 5.8S rRNA and 5S rRNA was reduced in mtr4 samples (Figure 8). Although the diminished rate of rRNA accumulation cannot be attributed with certainty to delayed 5.8S rRNA maturation, these data indicate that down-regulation of MTR4 impairs the de novo accumulation rate of mature rRNAs
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed ArticleWe observed a faint fluorescent signal in the nucleoplasm and a strong signal in nucleoli in all cells, suggesting that AtMTR4 is predominantly a nucleolar protein
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed Articlefragments of approximately 0.4–1.7 kb, representing degradation intermediates of the 5′ ETS, accumulated ... to higher levels in ... fry1 plants
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed ArticleThe S1 probe detected 35S* precursor transcripts comprising the entire 5′ ETS, all three rRNAs and the 3′ ETS in xrn2 ... mutants
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed ArticleTo visualize the expression of MTR4 in plant tissues, we expressed a GUS reporter gene under the control of 2 kb of genomic sequence upstream of the MTR4 gene. In agreement with publicly available microarray data (http://www.Genevestigator.com), we detected MTR4 promoter activity in all organs ... such as young leaves
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed ArticleTo examine the possibility that accumulation of illegitimate small RNAs accounts for the developmental phenotype of mtr4 mutants, we aimed to abrogate the production of 5′ ETS-derived small RNAs by introducing the mtr4 mutation into a dcl2 dcl4 background (Xie et al., 2005). Analysis of the small RNA fraction confirmed that production of 21 nt small RNAs from the 5′ ETS is suppressed in mtr4 dcl2 dcl4 triple mutants
Lange H, Sement FM, Gagliardi D - MTR4, a putative RNA helicase and exosome co-factor, is required for proper rRNA biogenesis and development in Arabidopsis thaliana
Visit the PubMed ArticleTo test whether STM was directly regulating the expression of CUC1, we turned again to the STM-GR system. Direct targets of STM-GR should be induced after DEX treatment, even in the presence of the translational inhibitor, cycloheximide (CYC). First, we crossed STM-GR transgenics to a CUC1 reporter line (PromCUC1:GUS). We used 1.4-kb CUC1 upstream sequences that have already been described to be sufficient to complement a cuc1 cuc2 mutant when fused to its coding sequence (Baker et al., 2005). The CUC1 promoter is normally expressed in the apical region, but DEX treatment during 24 h caused a strong induction in whole seedlings (Fig. 3A). As a control of the experimental approach, we observed that the supplemental addition of CYC largely prevented the burst of GUS protein activity, which is expected from the inhibition of the translational machinery (Fig. 3A). Note that these experiments were carried out under long induction and staining periods to ensure the saturation of the system
Spinelli SV, Martin AP, Viola IL, Gonzalez DH, Palatnik JF - A mechanistic link between STM and CUC1 during Arabidopsis development
Visit the PubMed ArticleAnalysis of ... CUC2 reporters in PromLFY:STM plants revealed that they were ... ectopically expressed in young leaves, especially at the base of leaf lobes
Spinelli SV, Martin AP, Viola IL, Gonzalez DH, Palatnik JF - A mechanistic link between STM and CUC1 during Arabidopsis development
Visit the PubMed ArticleThat the VP16 activated version caused stronger phenotypic defects than STM alone, suggested that the group of genes moderately induced by STM but strongly by STM-VP16 (Fig. 1D, cluster no. 2; Supplemental Table S4) might be particularly related to the KNOXI pathway. CUC1 (At3g15170) was included in this group and stood out as a particularly attractive candidate to study in more detail due to its known roles in the establishment of Arabidopsis meristem ... STM was able to induce CUC1 levels 4-fold, while STM-VP16 further enhanced the response to more than 100-fold in the microarray experiments
Spinelli SV, Martin AP, Viola IL, Gonzalez DH, Palatnik JF - A mechanistic link between STM and CUC1 during Arabidopsis development
Visit the PubMed ArticlePromLFY:STM plants ... We then determined the levels of CUC genes ... by RT-PCR in the proximal and distal region of the organ. The levels of CUC1 ... were increased in the proximal part of the organ, as expected from the whole-mount staining
Spinelli SV, Martin AP, Viola IL, Gonzalez DH, Palatnik JF - A mechanistic link between STM and CUC1 during Arabidopsis development
Visit the PubMed Articleexpression levels determined by RT-PCR in STM-GR transgenics ... CUC3 was also activated, but after 24 h. Supplemental addition of CYC prevented the induction of CUC3
Spinelli SV, Martin AP, Viola IL, Gonzalez DH, Palatnik JF - A mechanistic link between STM and CUC1 during Arabidopsis development
Visit the PubMed ArticlePromLFY:STM plants ... We then determined the levels of CUC genes ... by RT-PCR in the proximal and distal region of the organ. The levels of CUC1-3 ... were increased in the proximal part of the organ, as expected from the whole-mount staining
Spinelli SV, Martin AP, Viola IL, Gonzalez DH, Palatnik JF - A mechanistic link between STM and CUC1 during Arabidopsis development
Visit the PubMed ArticleWe then prepared an activated version of KNAT1, by fusing to it the VP16 domain. In this case, we observed that the induction of KNAT1-VP16 caused the activation of CUC1
Spinelli SV, Martin AP, Viola IL, Gonzalez DH, Palatnik JF - A mechanistic link between STM and CUC1 during Arabidopsis development
Visit the PubMed ArticleAnalysis of CUC1 ... reporters in PromLFY:STM plants revealed that they were ... ectopically expressed in young leaves, especially at the base of leaf lobes
Spinelli SV, Martin AP, Viola IL, Gonzalez DH, Palatnik JF - A mechanistic link between STM and CUC1 during Arabidopsis development
Visit the PubMed ArticleWe then tested the short-term response of miR164 to STM levels. We measured miR164 12 h after the induction of STM (Fig. 7E). However, in this case we did not observe an obvious change in the levels of the miRNA
Spinelli SV, Martin AP, Viola IL, Gonzalez DH, Palatnik JF - A mechanistic link between STM and CUC1 during Arabidopsis development
Visit the PubMed Articleprepared ethanol-inducible transgenic lines harboring ... KNAT2 ... failed to up-regulate CUC1
Spinelli SV, Martin AP, Viola IL, Gonzalez DH, Palatnik JF - A mechanistic link between STM and CUC1 during Arabidopsis development
Visit the PubMed ArticlePromLFY:STM plants ... We also determined the levels of the precursor of MIR164a and found that it was also activated, demonstrating its increased transcription is at least partially responsible for the elevated miR164 levels
Spinelli SV, Martin AP, Viola IL, Gonzalez DH, Palatnik JF - A mechanistic link between STM and CUC1 during Arabidopsis development
Visit the PubMed Articleprepared ethanol-inducible transgenic lines harboring KNAT1 ... failed to up-regulate CUC1
Spinelli SV, Martin AP, Viola IL, Gonzalez DH, Palatnik JF - A mechanistic link between STM and CUC1 during Arabidopsis development
Visit the PubMed ArticleCUC2 ... lack obvious STM binding sites
Spinelli SV, Martin AP, Viola IL, Gonzalez DH, Palatnik JF - A mechanistic link between STM and CUC1 during Arabidopsis development
Visit the PubMed ArticleWUS ... that were prepared as similar inducible versions ... failed to modify the levels of CUC2
Spinelli SV, Martin AP, Viola IL, Gonzalez DH, Palatnik JF - A mechanistic link between STM and CUC1 during Arabidopsis development
Visit the PubMed Articleexpression levels determined by RT-PCR in STM-GR transgenics ... We observed an effect on CUC2 only 96 h after DEX treatment (Fig. 3B). The longer activation time, which is prevented by incubation with CYC suggests that ... CUC3 are indirectly regulated by STM
Spinelli SV, Martin AP, Viola IL, Gonzalez DH, Palatnik JF - A mechanistic link between STM and CUC1 during Arabidopsis development
Visit the PubMed ArticlePromLFY:STM plants ... We then determined the levels of CUC genes ... by RT-PCR in the proximal and distal region of the organ. The levels of CUC1-3 ... were increased in the proximal part of the organ, as expected from the whole-mount staining
Spinelli SV, Martin AP, Viola IL, Gonzalez DH, Palatnik JF - A mechanistic link between STM and CUC1 during Arabidopsis development
Visit the PubMed ArticleWe then analyzed the expression of CUC genes at the RNA level ... We observed that 4 h of induction of STM-GR caused the up-regulation of CUC1 ... The longer activation time, which is prevented by incubation with CYC suggests that ... CUC2 ... indirectly regulated by STM
Spinelli SV, Martin AP, Viola IL, Gonzalez DH, Palatnik JF - A mechanistic link between STM and CUC1 during Arabidopsis development
Visit the PubMed ArticleLFY that were prepared as similar inducible versions ... failed to modify the levels of ... CUC3
Spinelli SV, Martin AP, Viola IL, Gonzalez DH, Palatnik JF - A mechanistic link between STM and CUC1 during Arabidopsis development
Visit the PubMed ArticleWUS ... that were prepared as similar inducible versions ... failed to modify the levels of ... CUC3
Spinelli SV, Martin AP, Viola IL, Gonzalez DH, Palatnik JF - A mechanistic link between STM and CUC1 during Arabidopsis development
Visit the PubMed ArticleWUS ... that were prepared as similar inducible versions ... not able to induce CUC1
Spinelli SV, Martin AP, Viola IL, Gonzalez DH, Palatnik JF - A mechanistic link between STM and CUC1 during Arabidopsis development
Visit the PubMed ArticleLFY that were prepared as similar inducible versions ... not able to induce CUC1
Spinelli SV, Martin AP, Viola IL, Gonzalez DH, Palatnik JF - A mechanistic link between STM and CUC1 during Arabidopsis development
Visit the PubMed Articleinducible ... STM-VP16 caused a moderate up-regulation of CUC3
Spinelli SV, Martin AP, Viola IL, Gonzalez DH, Palatnik JF - A mechanistic link between STM and CUC1 during Arabidopsis development
Visit the PubMed ArticleWe then analyzed the expression of CUC1 in the strong stm-1 mutant. As expected, we found that the levels of CUC1 were reduced in this mutant
Spinelli SV, Martin AP, Viola IL, Gonzalez DH, Palatnik JF - A mechanistic link between STM and CUC1 during Arabidopsis development
Visit the PubMed ArticleWe performed a transcriptome analysis on ATH1 microarrays, 12 h after the induction of STM and STM-VP16 ... IPT7, which participates in cytokinin biosynthesis, was induced nearly three times by STM, although it did not pass the logit-T filter used for the analysis of the arrays
Spinelli SV, Martin AP, Viola IL, Gonzalez DH, Palatnik JF - A mechanistic link between STM and CUC1 during Arabidopsis development
Visit the PubMed ArticleWe performed a transcriptome analysis on ATH1 microarrays, 12 h after the induction of STM and STM-VP16 ... At1g50960, which encodes a GA 2-oxidase7 involved in the catabolism of gibberellin, was significantly induced by both STM and STM-VP16
Spinelli SV, Martin AP, Viola IL, Gonzalez DH, Palatnik JF - A mechanistic link between STM and CUC1 during Arabidopsis development
Visit the PubMed ArticleLFY that were prepared as similar inducible versions ... failed to modify the levels of CUC2
Spinelli SV, Martin AP, Viola IL, Gonzalez DH, Palatnik JF - A mechanistic link between STM and CUC1 during Arabidopsis development
Visit the PubMed Articlewe crossed PromLFY:STM plants to a MIR164a reporter. We found that MIR164a was also activated by STM, in a similar way to the CUC reporters
Spinelli SV, Martin AP, Viola IL, Gonzalez DH, Palatnik JF - A mechanistic link between STM and CUC1 during Arabidopsis development
Visit the PubMed ArticleTo analyze the expression pattern of the FYF gene during Arabidopsis development, total RNA was extracted from different growth stages (7- and 14-day-old plants) and different parts of 45-day-old mature wild-type Arabidopsis for real-time quantitative RT-PCR analysis. As shown in Figure 2d, the expression of the FYF gene was barely detected in 7-day-old seedlings after germination, and the expression level was slightly increased in 14-day-old plants. In 45-day-old mature plants, the FYF gene was expressed in all of the tissues tested (i.e. the root, rosette leaf, stem, cauline leaf, flower and silique) with a relatively higher expression level in the rosette and cauline leaves than in the flowers
Chen MK, Hsu WH, Lee PF, Thiruvengadam M, Chen HI, Yang CH. - The MADS box gene, FOREVER YOUNG FLOWER, acts as a repressor controlling floral organ senescence and abscission in Arabidopsis
Visit the PubMed ArticleArabidopsis eFP browser data (Schmid et al., 2005; Winter et al., 2007; http://www.bar.utoronto.ca/efp/cgi-bin/efpWeb.cgi ), which report that FYF is barely detected in the leaves of young plants but is highly expressed in leaves during a late developmental stage
Chen MK, Hsu WH, Lee PF, Thiruvengadam M, Chen HI, Yang CH. - The MADS box gene, FOREVER YOUNG FLOWER, acts as a repressor controlling floral organ senescence and abscission in Arabidopsis
Visit the PubMed ArticleGUS activity was also detected at the base of the petiole of the cotyledons and leaves in FYF:GUS seedlings (Figure 3k,l) and young plants (Figure 3m,n), though GUS activity was barely detected in the rosette leaves during the early developmental stages
Chen MK, Hsu WH, Lee PF, Thiruvengadam M, Chen HI, Yang CH. - The MADS box gene, FOREVER YOUNG FLOWER, acts as a repressor controlling floral organ senescence and abscission in Arabidopsis
Visit the PubMed ArticleFurthermore, up-regulation of the pathogenesis-related genes PR1, PR2, and PR5 was observed in vdac2-2 and vdac4-2, but not in vdac1-6 and vdac3-3, under normal conditions (Fig. 4G
Tateda C, Watanabe K, Kusano T, Takahashi Y - Molecular and genetic characterization of the gene family encoding the voltage-dependent anion channel in Arabidopsis
Visit the PubMed ArticleTo examine the relationship between VDAC function and defence activity against the bacterial pathogen Pst, the expression profiles of VDAC genes during pathogen infection were analysed using quantitative RT-PCR. All four VDAC members were up-regulated upon infection with the bacterial pathogen. The relative transcript levels of VDAC1 and VDAC3 were enhanced to a greater degree when plants were infected with the avirulence pathogen, Pst DC3000 (avrRpt2), than with the virulence pathogen, Pst DC3000
Tateda C, Watanabe K, Kusano T, Takahashi Y - Molecular and genetic characterization of the gene family encoding the voltage-dependent anion channel in Arabidopsis
Visit the PubMed ArticleVDAC1–GFP and VDAC3–GFP were exclusively detected in mitochondria
Tateda C, Watanabe K, Kusano T, Takahashi Y - Molecular and genetic characterization of the gene family encoding the voltage-dependent anion channel in Arabidopsis
Visit the PubMed ArticleThe VDAC4 promoter was constitutively active in whole seedlings (Fig. 3O) and in almost all organs (Fig. 3P–S
Tateda C, Watanabe K, Kusano T, Takahashi Y - Molecular and genetic characterization of the gene family encoding the voltage-dependent anion channel in Arabidopsis
Visit the PubMed ArticleFurthermore, up-regulation of the pathogenesis-related genes PR1, PR2, and PR5 was observed in vdac2-2 and vdac4-2, but not in vdac1-6 and vdac3-3, under normal conditions (Fig. 4G
Tateda C, Watanabe K, Kusano T, Takahashi Y - Molecular and genetic characterization of the gene family encoding the voltage-dependent anion channel in Arabidopsis
Visit the PubMed ArticleIn contrast, the green fluorescence signals of VDAC2–GFP and VDCA4–GFP were detected not only in mitochondria, but also in another subcellular area
Tateda C, Watanabe K, Kusano T, Takahashi Y - Molecular and genetic characterization of the gene family encoding the voltage-dependent anion channel in Arabidopsis
Visit the PubMed ArticleAs a step toward determining the functional sites of the four VDAC genes in planta, transgenic Arabidopsis plants carrying the respective VDAC promoter–GUS constructs were generated, and the spatiotemporal expression pattern of the reporter gene was evaluated. The VDAC1 promoter was active in shoot meristems and the surrounding regions (Fig. 3A), corresponding to the root meristematic zone, but not in root caps (Fig. 3B), lateral roots (Fig. 3C), the entire leaf area (Fig. 3D), and the stigma and anthers (Fig. 3E
Tateda C, Watanabe K, Kusano T, Takahashi Y - Molecular and genetic characterization of the gene family encoding the voltage-dependent anion channel in Arabidopsis
Visit the PubMed ArticleAs a step toward determining the functional sites of the four VDAC genes in planta, transgenic Arabidopsis plants carrying the respective VDAC promoter–GUS constructs were generated, and the spatiotemporal expression pattern of the reporter gene was evaluated. The VDAC1 promoter was active in shoot meristems and the surrounding regions (Fig. 3A), corresponding to the root meristematic zone, but not in root caps (Fig. 3B), lateral roots (Fig. 3C), the entire leaf area (Fig. 3D), and the stigma and anthers (Fig. 3E
Tateda C, Watanabe K, Kusano T, Takahashi Y - Molecular and genetic characterization of the gene family encoding the voltage-dependent anion channel in Arabidopsis
Visit the PubMed ArticleVDAC1–GFP and VDAC3–GFP were exclusively detected in mitochondria
Tateda C, Watanabe K, Kusano T, Takahashi Y - Molecular and genetic characterization of the gene family encoding the voltage-dependent anion channel in Arabidopsis
Visit the PubMed ArticleFurthermore, up-regulation of the pathogenesis-related genes PR1, PR2, and PR5 was observed in vdac2-2 and vdac4-2, but not in vdac1-6 and vdac3-3, under normal conditions (Fig. 4G
Tateda C, Watanabe K, Kusano T, Takahashi Y - Molecular and genetic characterization of the gene family encoding the voltage-dependent anion channel in Arabidopsis
Visit the PubMed ArticleTo examine the relationship between VDAC function and defence activity against the bacterial pathogen Pst, the expression profiles of VDAC genes during pathogen infection were analysed using quantitative RT-PCR. All four VDAC members were up-regulated upon infection with the bacterial pathogen. The relative transcript levels of VDAC1 and VDAC3 were enhanced to a greater degree when plants were infected with the avirulence pathogen, Pst DC3000 (avrRpt2), than with the virulence pathogen, Pst DC3000
Tateda C, Watanabe K, Kusano T, Takahashi Y - Molecular and genetic characterization of the gene family encoding the voltage-dependent anion channel in Arabidopsis
Visit the PubMed ArticleFurthermore, up-regulation of the pathogenesis-related genes PR1, PR2, and PR5 was observed in vdac2-2 and vdac4-2, but not in vdac1-6 and vdac3-3, under normal conditions (Fig. 4G
Tateda C, Watanabe K, Kusano T, Takahashi Y - Molecular and genetic characterization of the gene family encoding the voltage-dependent anion channel in Arabidopsis
Visit the PubMed ArticleVDAC1 or VDAC4 expression was totally suppressed in vdac1-6 or vdac4-2 plants, and VDAC2 or VDAC3 expression decreased to 27% or 23% in vdac2-2 or vdac3-3, respectively, compared with that of the WT (Fig. 4D
Tateda C, Watanabe K, Kusano T, Takahashi Y - Molecular and genetic characterization of the gene family encoding the voltage-dependent anion channel in Arabidopsis
Visit the PubMed ArticleFurthermore, up-regulation of the pathogenesis-related genes PR1, PR2, and PR5 was observed in vdac2-2 and vdac4-2, but not in vdac1-6 and vdac3-3, under normal conditions (Fig. 4G
Tateda C, Watanabe K, Kusano T, Takahashi Y - Molecular and genetic characterization of the gene family encoding the voltage-dependent anion channel in Arabidopsis
Visit the PubMed ArticleVDAC3 promoter activity was scarcely detected in leaves, except for the leaf tips (Fig. 3K–M), and rather strong activity was detected in the anthers and stigma (Fig. 3N
Tateda C, Watanabe K, Kusano T, Takahashi Y - Molecular and genetic characterization of the gene family encoding the voltage-dependent anion channel in Arabidopsis
Visit the PubMed ArticleFurthermore, up-regulation of the pathogenesis-related genes PR1, PR2, and PR5 was observed in vdac2-2 and vdac4-2, but not in vdac1-6 and vdac3-3, under normal conditions (Fig. 4G
Tateda C, Watanabe K, Kusano T, Takahashi Y - Molecular and genetic characterization of the gene family encoding the voltage-dependent anion channel in Arabidopsis
Visit the PubMed ArticleThe VDAC4 promoter was constitutively active in whole seedlings (Fig. 3O) and in almost all organs (Fig. 3P–S
Tateda C, Watanabe K, Kusano T, Takahashi Y - Molecular and genetic characterization of the gene family encoding the voltage-dependent anion channel in Arabidopsis
Visit the PubMed ArticleThe activity of the VDAC2 promoter was constitutively elevated in the above-ground parts of the plant (Fig. 3F) and in the root tips (Fig. 3G) and steles (Fig. 3H). The activity was detected in whole leaves of the plants which had developed further (Fig. 3I) and in whole flower organs, including sepals (Fig. 3J
Tateda C, Watanabe K, Kusano T, Takahashi Y - Molecular and genetic characterization of the gene family encoding the voltage-dependent anion channel in Arabidopsis
Visit the PubMed ArticleFurthermore, up-regulation of the pathogenesis-related genes PR1, PR2, and PR5 was observed in vdac2-2 and vdac4-2, but not in vdac1-6 and vdac3-3, under normal conditions (Fig. 4G
Tateda C, Watanabe K, Kusano T, Takahashi Y - Molecular and genetic characterization of the gene family encoding the voltage-dependent anion channel in Arabidopsis
Visit the PubMed ArticleVDAC1 or VDAC4 expression was totally suppressed in vdac1-6 or vdac4-2 plants, and VDAC2 or VDAC3 expression decreased to 27% or 23% in vdac2-2 or vdac3-3, respectively, compared with that of the WT (Fig. 4D
Tateda C, Watanabe K, Kusano T, Takahashi Y - Molecular and genetic characterization of the gene family encoding the voltage-dependent anion channel in Arabidopsis
Visit the PubMed ArticleTo examine the relationship between VDAC function and defence activity against the bacterial pathogen Pst, the expression profiles of VDAC genes during pathogen infection were analysed using quantitative RT-PCR. All four VDAC members were up-regulated upon infection with the bacterial pathogen. The relative transcript levels of VDAC1 and VDAC3 were enhanced to a greater degree when plants were infected with the avirulence pathogen, Pst DC3000 (avrRpt2), than with the virulence pathogen, Pst DC3000
Tateda C, Watanabe K, Kusano T, Takahashi Y - Molecular and genetic characterization of the gene family encoding the voltage-dependent anion channel in Arabidopsis
Visit the PubMed ArticleFurthermore, up-regulation of the pathogenesis-related genes PR1, PR2, and PR5 was observed in vdac2-2 and vdac4-2, but not in vdac1-6 and vdac3-3, under normal conditions (Fig. 4G
Tateda C, Watanabe K, Kusano T, Takahashi Y - Molecular and genetic characterization of the gene family encoding the voltage-dependent anion channel in Arabidopsis
Visit the PubMed ArticleThe activity of the VDAC2 promoter was constitutively elevated in the above-ground parts of the plant (Fig. 3F) and in the root tips (Fig. 3G) and steles (Fig. 3H). The activity was detected in whole leaves of the plants which had developed further (Fig. 3I) and in whole flower organs, including sepals (Fig. 3J
Tateda C, Watanabe K, Kusano T, Takahashi Y - Molecular and genetic characterization of the gene family encoding the voltage-dependent anion channel in Arabidopsis
Visit the PubMed ArticleIn contrast, the green fluorescence signals of VDAC2–GFP and VDCA4–GFP were detected not only in mitochondria, but also in another subcellular area
Tateda C, Watanabe K, Kusano T, Takahashi Y - Molecular and genetic characterization of the gene family encoding the voltage-dependent anion channel in Arabidopsis
Visit the PubMed ArticleVDAC1 or VDAC4 expression was totally suppressed in vdac1-6 or vdac4-2 plants, and VDAC2 or VDAC3 expression decreased to 27% or 23% in vdac2-2 or vdac3-3, respectively, compared with that of the WT (Fig. 4D
Tateda C, Watanabe K, Kusano T, Takahashi Y - Molecular and genetic characterization of the gene family encoding the voltage-dependent anion channel in Arabidopsis
Visit the PubMed ArticleFurthermore, up-regulation of the pathogenesis-related genes PR1, PR2, and PR5 was observed in vdac2-2 and vdac4-2, but not in vdac1-6 and vdac3-3, under normal conditions (Fig. 4G
Tateda C, Watanabe K, Kusano T, Takahashi Y - Molecular and genetic characterization of the gene family encoding the voltage-dependent anion channel in Arabidopsis
Visit the PubMed ArticleFurthermore, up-regulation of the pathogenesis-related genes PR1, PR2, and PR5 was observed in vdac2-2 and vdac4-2, but not in vdac1-6 and vdac3-3, under normal conditions (Fig. 4G
Tateda C, Watanabe K, Kusano T, Takahashi Y - Molecular and genetic characterization of the gene family encoding the voltage-dependent anion channel in Arabidopsis
Visit the PubMed ArticleFurthermore, up-regulation of the pathogenesis-related genes PR1, PR2, and PR5 was observed ... observed in vdac2-2 and vdac4-2, but not in vdac1-6 and vdac3-3, under normal conditions (Fig. 4G
Tateda C, Watanabe K, Kusano T, Takahashi Y - Molecular and genetic characterization of the gene family encoding the voltage-dependent anion channel in Arabidopsis
Visit the PubMed ArticleFurthermore, up-regulation of the pathogenesis-related genes PR1, PR2, and PR5 was observed in vdac2-2 and vdac4-2, but not in vdac1-6 and vdac3-3, under normal conditions (Fig. 4G
Tateda C, Watanabe K, Kusano T, Takahashi Y - Molecular and genetic characterization of the gene family encoding the voltage-dependent anion channel in Arabidopsis
Visit the PubMed ArticleTo examine the relationship between VDAC function and defence activity against the bacterial pathogen Pst, the expression profiles of VDAC genes during pathogen infection were analysed using quantitative RT-PCR. All four VDAC members were up-regulated upon infection with the bacterial pathogen. The relative transcript levels of VDAC1 and VDAC3 were enhanced to a greater degree when plants were infected with the avirulence pathogen, Pst DC3000 (avrRpt2), than with the virulence pathogen, Pst DC3000
Tateda C, Watanabe K, Kusano T, Takahashi Y - Molecular and genetic characterization of the gene family encoding the voltage-dependent anion channel in Arabidopsis
Visit the PubMed ArticleVDAC1 or VDAC4 expression was totally suppressed in vdac1-6 or vdac4-2 plants, and VDAC2 or VDAC3 expression decreased to 27% or 23% in vdac2-2 or vdac3-3, respectively, compared with that of the WT (Fig. 4D
Tateda C, Watanabe K, Kusano T, Takahashi Y - Molecular and genetic characterization of the gene family encoding the voltage-dependent anion channel in Arabidopsis
Visit the PubMed ArticleBiP2-promoter GUS construct (Oh et al., 2003) expressed in alg10-1 resulted in a strong GUS signal throughout the whole seedling
Farid A, Pabst M, Schoberer J, Altmann F, Glössl J, Strasser R. - Arabidopsis thaliana alpha1,2-glucosyltransferase (ALG10) is required for efficient N-glycosylation and leaf growth
Visit the PubMed ArticleWe amplified the whole open reading frame including additional 5′- and 3′-untranslated regions of the Arabidopsis ALG10 from leaf cDNA
Farid A, Pabst M, Schoberer J, Altmann F, Glössl J, Strasser R. - Arabidopsis thaliana alpha1,2-glucosyltransferase (ALG10) is required for efficient N-glycosylation and leaf growth
Visit the PubMed ArticleTo determine its subcellular localization ALG10 was fused to GFP and transiently expressed in N. benthamiana leaf epidermal cells. Analysis of the ALG10–GFP fusion protein by confocal laser scanning microscopy revealed a reticular distribution pattern resembling ER localization (Figure 2). To confirm the localization, we co-expressed ALG10–GFP with the ER-retained GnTI-CaaaTS-mRFP, a mutated fusion protein that mainly localizes to the ER with a minor portion concentrating in the Golgi (Figure 2) (Schoberer et al., 2009). Most of ALG10–GFP displayed co-localization with GnTI-CaaaTS-mRFP, which is in agreement with the proposed function of the enzyme in the assembly of the dolichol-linked oligosaccharide precursor in the ER
Farid A, Pabst M, Schoberer J, Altmann F, Glössl J, Strasser R. - Arabidopsis thaliana alpha1,2-glucosyltransferase (ALG10) is required for efficient N-glycosylation and leaf growth
Visit the PubMed ArticleThe gene expression profiles from the Bio-Array Resource for Plant Functional Genomics (BAR; http://bbc.botany.utoronto.ca/efp/cgi-bin/efpWeb.cgi) and Genevestigator (https://www.genevestigator.com/gv/index.jsp) indicate that ALG10 expression is high in roots, stems and leaves and reduced in pollen, embryos and endosperm
Farid A, Pabst M, Schoberer J, Altmann F, Glössl J, Strasser R. - Arabidopsis thaliana alpha1,2-glucosyltransferase (ALG10) is required for efficient N-glycosylation and leaf growth
Visit the PubMed ArticleWe hypothesized that underglycosylation of proteins in alg10-1 should lead to activation of the UPR. In accordance with our prediction, the expression of the folding chaperone binding protein (BiP) was increased in the alg10-1 mutant
Farid A, Pabst M, Schoberer J, Altmann F, Glössl J, Strasser R. - Arabidopsis thaliana alpha1,2-glucosyltransferase (ALG10) is required for efficient N-glycosylation and leaf growth
Visit the PubMed ArticleTo analyze the expression pattern in plant tissues, a 2.9-kb fragment upstream of the ATG codon of APC10 was fused to the β-glucuronidase (GUS) reporter gene by means of a FAST vector ... A cross-section through the shoot apical meristem (Figure 2b) showed that APC10 was expressed in all cells
Eloy NB, de Freitas Lima M, Van Damme D, Vanhaeren H, Gonzalez N, De Milde L, Hemerly AS, Beemster GT, Inzé D, Ferreira PC. - The APC/C subunit 10 plays an essential role in cell proliferation during leaf development
Visit the PubMed Articlein the APC10OE plants ... Prol1_At5g16250 ... up-regulated
Eloy NB, de Freitas Lima M, Van Damme D, Vanhaeren H, Gonzalez N, De Milde L, Hemerly AS, Beemster GT, Inzé D, Ferreira PC. - The APC/C subunit 10 plays an essential role in cell proliferation during leaf development
Visit the PubMed ArticleTo determine the subcellular localization of the APC10 protein, it was stably produced as a C-terminal protein fused to the green fluorescent protein (GFP), driven by the cauliflower mosaic virus (CaMV) 35S promoter in Arabidopsis seedlings. The APC10–GFP fusion protein was functional because it was able to complement the fission yeast apc10ts strain at a non-permissive temperature (data not shown). In the root meristem, the APC10–GFP fluorescence was predominantly located in the nucleus, excluded from the nucleolus and, to a minor extent, also present in the cytoplasm. Several small fluorescent dots were observed inside the nucleus as well (Figure 3a, arrows). These punctuations resembled nuclear bodies, probably representing the association of APC10 with the proteasome complex (Tao et al., 2005). In the root elongation zone, the nuclear bodies could also be visualized, although to a lesser extent than in the root tip (Figure 3b,c). In leaf pavement cells and trichomes, the APC10–GFP fluorescence had the same pattern as in root cells, including the presence of nuclear bodies
Eloy NB, de Freitas Lima M, Van Damme D, Vanhaeren H, Gonzalez N, De Milde L, Hemerly AS, Beemster GT, Inzé D, Ferreira PC. - The APC/C subunit 10 plays an essential role in cell proliferation during leaf development
Visit the PubMed Articlein the APC10OE plants ... Prol2_At3g42660 ... up-regulated
Eloy NB, de Freitas Lima M, Van Damme D, Vanhaeren H, Gonzalez N, De Milde L, Hemerly AS, Beemster GT, Inzé D, Ferreira PC. - The APC/C subunit 10 plays an essential role in cell proliferation during leaf development
Visit the PubMed ArticleLeaves of 20 F1 plants from each cross (APC10OE × pCYCB1;1:D-box–GUS and Col-0 × pCYCB1;1:D-box–GUS) were harvested, stained for GUS activity and cleared with lactic acid. Photographs were taken and the GUS staining intensity quantified with ImageJ software (see Experimental Procedures). First, in plants at 7 DAS, in which leaves 1 and 2 were entirely proliferating (Figure 6), the GUS staining intensity was clearly reduced (Figure 8a,c). In leaf 3 at 12 DAS, in which the transition between cell proliferation and cell expansion is clear, a reduced GUS staining intensity was visible (Figure 8b,d). As this reduced GUS staining was not caused by reduced CYCB1;1 expression (Figure 7c), the data strongly suggest that APC10 overexpression enhances the D-box-dependent proteolysis of CYCB1;1
Eloy NB, de Freitas Lima M, Van Damme D, Vanhaeren H, Gonzalez N, De Milde L, Hemerly AS, Beemster GT, Inzé D, Ferreira PC. - The APC/C subunit 10 plays an essential role in cell proliferation during leaf development
Visit the PubMed ArticleTo analyze the expression pattern in plant tissues, a 2.9-kb fragment upstream of the ATG codon of APC10 was fused to the β-glucuronidase (GUS) reporter gene by means of a FAST vector ... When leaf development progressed, the APC10-GUS expression gradually diminished, but remained high in vascular tissues
Eloy NB, de Freitas Lima M, Van Damme D, Vanhaeren H, Gonzalez N, De Milde L, Hemerly AS, Beemster GT, Inzé D, Ferreira PC. - The APC/C subunit 10 plays an essential role in cell proliferation during leaf development
Visit the PubMed ArticleTo analyze the expression pattern in plant tissues, a 2.9-kb fragment upstream of the ATG codon of APC10 was fused to the β-glucuronidase (GUS) reporter gene by means of a FAST vector ... Staining for GUS activity revealed a strong and more or less constitutive expression in young leaves, hypocotyls, female organs and roots with the remarkable exception of the root apical region (Figure 2). A similar expression pattern was obtained when the 2.9-kb promoter fragment and the entire coding sequence, including the five introns, was fused at the stop codon to the GUS reporter and analyzed in transgenic plants
Eloy NB, de Freitas Lima M, Van Damme D, Vanhaeren H, Gonzalez N, De Milde L, Hemerly AS, Beemster GT, Inzé D, Ferreira PC. - The APC/C subunit 10 plays an essential role in cell proliferation during leaf development
Visit the PubMed ArticleLeaf 3 was harvested and analyzed by qPCR. Consistent with the stimulation of G2/M, the data showed that the transcript level of ... CDKB2;1 ... had increased in the APC10OE plants
Eloy NB, de Freitas Lima M, Van Damme D, Vanhaeren H, Gonzalez N, De Milde L, Hemerly AS, Beemster GT, Inzé D, Ferreira PC. - The APC/C subunit 10 plays an essential role in cell proliferation during leaf development
Visit the PubMed ArticleThe analysis of APC10 expression in a wide range of microarrays suggested that it is constitutively expressed during plant development and throughout the cell cycle
Eloy NB, de Freitas Lima M, Van Damme D, Vanhaeren H, Gonzalez N, De Milde L, Hemerly AS, Beemster GT, Inzé D, Ferreira PC. - The APC/C subunit 10 plays an essential role in cell proliferation during leaf development
Visit the PubMed ArticleLeaf 3 was harvested and analyzed by qPCR. Consistent with the stimulation of G2/M, the data showed that the transcript level of ... BUB3.1 ... had increased in the APC10OE plants
Eloy NB, de Freitas Lima M, Van Damme D, Vanhaeren H, Gonzalez N, De Milde L, Hemerly AS, Beemster GT, Inzé D, Ferreira PC. - The APC/C subunit 10 plays an essential role in cell proliferation during leaf development
Visit the PubMed ArticleTo analyze the expression pattern in plant tissues, a 2.9-kb fragment upstream of the ATG codon of APC10 was fused to the β-glucuronidase (GUS) reporter gene by means of a FAST vector ... reduced expression was observed at the tip of developing true leaves, most probably reflecting reduced expression of APC10 in cells that start to differentiate
Eloy NB, de Freitas Lima M, Van Damme D, Vanhaeren H, Gonzalez N, De Milde L, Hemerly AS, Beemster GT, Inzé D, Ferreira PC. - The APC/C subunit 10 plays an essential role in cell proliferation during leaf development
Visit the PubMed Articlethe expression of HB33 was inhibited by ABA treatment in the wild type
Wang L, Hua D, He J, Duan Y, Chen Z, Hong X, Gong Z - Auxin Response Factor2 (ARF2) and its regulated homeodomain gene HB33 mediate abscisic acid response in Arabidopsis
Visit the PubMed Articlewe compared the HB33 expression of the wild type with ... arf21 mutant, and did not find the expression difference of HB33 between these mutants and the wild type (Figure 4B
Wang L, Hua D, He J, Duan Y, Chen Z, Hong X, Gong Z - Auxin Response Factor2 (ARF2) and its regulated homeodomain gene HB33 mediate abscisic acid response in Arabidopsis
Visit the PubMed ArticleIt appears that auxin treatment weakly induced the expression of HB33 in the early times in ... wild type
Wang L, Hua D, He J, Duan Y, Chen Z, Hong X, Gong Z - Auxin Response Factor2 (ARF2) and its regulated homeodomain gene HB33 mediate abscisic acid response in Arabidopsis
Visit the PubMed ArticleABA treatment increased the expression of ARF2 (relative to the control without ABA, from about 5 folds at 12 hr to 2 folds at 30 hr)
Wang L, Hua D, He J, Duan Y, Chen Z, Hong X, Gong Z - Auxin Response Factor2 (ARF2) and its regulated homeodomain gene HB33 mediate abscisic acid response in Arabidopsis
Visit the PubMed Articlewe compared the HB33 expression of the wild type with arf1 ... and did not find the expression difference of HB33 between these mutants and the wild type (Figure 4B
Wang L, Hua D, He J, Duan Y, Chen Z, Hong X, Gong Z - Auxin Response Factor2 (ARF2) and its regulated homeodomain gene HB33 mediate abscisic acid response in Arabidopsis
Visit the PubMed Articlewe compared the HB33 expression of the wild type with ... arf6 ... and did not find the expression difference of HB33 between these mutants and the wild type (Figure 4B
Wang L, Hua D, He J, Duan Y, Chen Z, Hong X, Gong Z - Auxin Response Factor2 (ARF2) and its regulated homeodomain gene HB33 mediate abscisic acid response in Arabidopsis
Visit the PubMed Articlethe expression of HB33 was inhibited by ABA treatment in the wild type but not in the arf2-101 mutant
Wang L, Hua D, He J, Duan Y, Chen Z, Hong X, Gong Z - Auxin Response Factor2 (ARF2) and its regulated homeodomain gene HB33 mediate abscisic acid response in Arabidopsis
Visit the PubMed ArticleWe also determined that the expression of HB33 was lower in two ARF2-flag overexpressing lines than in the wild type and was not further reduced by ABA treatment (Figure 3A)
Wang L, Hua D, He J, Duan Y, Chen Z, Hong X, Gong Z - Auxin Response Factor2 (ARF2) and its regulated homeodomain gene HB33 mediate abscisic acid response in Arabidopsis
Visit the PubMed ArticleIn searching for genes regulated by ARF2 from microarray data [21], we found that AT1G75240 encoding HOMEOBOX PROTEIN 33 (HB33) was up-regulated in the arf2 mutant (the average signal was 153 in the wild type and 276 in arf2-6 from three independent repeats) [21
Wang L, Hua D, He J, Duan Y, Chen Z, Hong X, Gong Z - Auxin Response Factor2 (ARF2) and its regulated homeodomain gene HB33 mediate abscisic acid response in Arabidopsis
Visit the PubMed ArticleWe confirmed the microarray results by qRT-PCR, which showed that the expression of HB33 was about 1.5 times greater in arf2-101 than in the wild type (Figure 3A)
Wang L, Hua D, He J, Duan Y, Chen Z, Hong X, Gong Z - Auxin Response Factor2 (ARF2) and its regulated homeodomain gene HB33 mediate abscisic acid response in Arabidopsis
Visit the PubMed ArticleIt appears that auxin treatment weakly induced the expression of HB33 in the early times in ... arf2 mutant
Wang L, Hua D, He J, Duan Y, Chen Z, Hong X, Gong Z - Auxin Response Factor2 (ARF2) and its regulated homeodomain gene HB33 mediate abscisic acid response in Arabidopsis
Visit the PubMed Articlemdo1-1 ... expression of CYCB1;2:GUS containing a destruction box, the expression of which is induced by the duration of the G2/M transition, but not by DNA damage responses (Culligan et al., 2006), was not drastically enhanced in the mutant shoot apex, and was instead slightly reduced in the mutant root tips
Hashimura Y, Ueguchi C - The Arabidopsis MERISTEM DISORGANIZATION 1 gene is required for the maintenance of stem cells through the reduction of DNA damage
Visit the PubMed ArticleMDO1 promoter:GUS fusion ... growing leaves
Hashimura Y, Ueguchi C - The Arabidopsis MERISTEM DISORGANIZATION 1 gene is required for the maintenance of stem cells through the reduction of DNA damage
Visit the PubMed ArticleMDO1 promoter:GUS fusion ... The vasculatures were also strongly stained throughout the whole plant
Hashimura Y, Ueguchi C - The Arabidopsis MERISTEM DISORGANIZATION 1 gene is required for the maintenance of stem cells through the reduction of DNA damage
Visit the PubMed ArticleTo examine the expression of the MDO1 gene, we constructed a MDO1 promoter:GUS fusion (MDO1pro:GUS) as a probe. As shown in Figure 9, the MDO1 gene is expressed nearly ubiquitously throughout the whole plant, but at a low level. In seedlings, strong staining was mainly observed in meristematic tissues, such as in the SAM
Hashimura Y, Ueguchi C - The Arabidopsis MERISTEM DISORGANIZATION 1 gene is required for the maintenance of stem cells through the reduction of DNA damage
Visit the PubMed ArticleAlthough ANT expression was downregulated in the wild-type central region (Figure 2h), it was detected in a wide range of cells in the mdo1-1 shoot apex
Hashimura Y, Ueguchi C - The Arabidopsis MERISTEM DISORGANIZATION 1 gene is required for the maintenance of stem cells through the reduction of DNA damage
Visit the PubMed Articlewe first examined the expression of the ... GAMMA RESPONSE 1 (ATGR1 ... Total RNA was prepared from shoot apex tissues and then subjected to quantitative real-time RT-PCR (qRT-PCR) analysis. It was revealed that, in mdo1-1, the transcript levels of these genes were increased 2–2.5-fold compared with those in the wild type
Hashimura Y, Ueguchi C - The Arabidopsis MERISTEM DISORGANIZATION 1 gene is required for the maintenance of stem cells through the reduction of DNA damage
Visit the PubMed Articlewe first examined the expression of the RAD51 ... Total RNA was prepared from shoot apex tissues and then subjected to quantitative real-time RT-PCR (qRT-PCR) analysis. It was revealed that, in mdo1-1, the transcript levels of these genes were increased 2–2.5-fold compared with those in the wild type
Hashimura Y, Ueguchi C - The Arabidopsis MERISTEM DISORGANIZATION 1 gene is required for the maintenance of stem cells through the reduction of DNA damage
Visit the PubMed Articlewe first examined the expression of the ... BREAST CANCER SUSCEPTIBILITY 1 (ATBRCA1 ... Total RNA was prepared from shoot apex tissues and then subjected to quantitative real-time RT-PCR (qRT-PCR) analysis. It was revealed that, in mdo1-1, the transcript levels of these genes were increased 2–2.5-fold compared with those in the wild type
Hashimura Y, Ueguchi C - The Arabidopsis MERISTEM DISORGANIZATION 1 gene is required for the maintenance of stem cells through the reduction of DNA damage
Visit the PubMed ArticleIn the mdo1-1 shoot apex, STM expression was reduced in the surface cells (Figure 2f), or, in some samples, multiple STM expression domains could be observed
Hashimura Y, Ueguchi C - The Arabidopsis MERISTEM DISORGANIZATION 1 gene is required for the maintenance of stem cells through the reduction of DNA damage
Visit the PubMed ArticlePlants carrying a CYCB1;1:GUS fusion containing a mitotic destruction box (Colón-Carmona et al., 1999) were subjected to GUS staining. The GUS activity was strongly enhanced by the mdo1-1 mutation in both shoot apices and root tips
Hashimura Y, Ueguchi C - The Arabidopsis MERISTEM DISORGANIZATION 1 gene is required for the maintenance of stem cells through the reduction of DNA damage
Visit the PubMed ArticleTo experimentally determine the subcellular localization of the RUG2 protein, stably transgenic Arabidopsis plants expressing a construct encoding the first 103 amino acid residues of RUG2 fused to GFP (RUG2-N-terminal:GFP) were obtained, and GFP signals were detected in large, oval-shaped subcellular structures completely overlapping with chlorophyll autofluorescence
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed Articlein our microarray ... upregulated ... in rug2-1 ... rpS4
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed ArticleqRT-PCR ... confirmed that in rug2-1 the matK ... upregulated ... 12.1
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed ArticleqRT-PCR ... confirmed that in rug2-1 ... rpoC2 ... upregulated ... 7.5
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed ArticleRUG2-N-terminal:GFP stably transgenic plants ... and were stained with MitoTracker Red CMX-ROS, a specific mitochondria fluorescence dye (see Experimental procedures ... green fluorescence signals in the punctate structures co-localized with the MitoTracker Red signals (Figure 3j,l), suggesting that RUG2 accumulates within the mitochondria
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed Articlerug2-1. In the chloroplastic genome ... psbN ... downregulated
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed Articlein our microarray ... upregulated ... in rug2-1 ... psbF
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed ArticleqRT-PCR ... confirmed that in rug2-1 ... rpoB ... upregulated ... 9.2
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed ArticleWe studied the spatial and temporal expression patterns of RUG2 in Col-0 by qRT-PCR, and detected RUG2 transcripts in all organs and stages analyzed (Figure S4 ... detected in vegetative leaves
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed Articlerug2-1 mutant ... DNA microarray ... orf149 ... downregulated
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed Articlein our microarray ... upregulated ... in rug2-1 ... ndhB.2
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed ArticleatpB (ATP synthase subunit ... investigated ... by western blot analysis ... accumulated in rug2-1
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed Articlein our microarray ... upregulated ... in rug2-1 ... matK
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed Articlein our microarray ... upregulated ... in rug2-1 ... rpS2
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed ArticleOnly three mitochondrial genes of unknown function (orf116 ... were upregulated in rug2-1
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed ArticleWe studied the spatial and temporal expression patterns of RUG2 in Col-0 by qRT-PCR, and detected RUG2 transcripts in all organs and stages analyzed ... detected in ... 4-day-old seedlings ... 1.7- and 2.6-fold higher than in 12- and 21-day-old plants, respectively
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed Articlein our microarray ... upregulated ... in rug2 ... 1 ... rpoB
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed ArticleTo examine the effect of rug2 mutations on the expression of the RUG2 gene, total RNA was extracted from 3-week-old Col-0, Ler, rug2-1 and rug2-2 plants, reverse transcribed and PCR amplified. Primers flanking the rug2-2 T-DNA insertion (F3 and R3 in Figure 2b) yielded amplification products only from Col-0, but not from rug2-2 cDNA or genomic DNA (Figure 2d
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed Articlerug2-1. In the chloroplastic genome ... psbA ... were all downregulated
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed Articlein our microarray ... upregulated ... in rug2-1 ... atpH
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed ArticleThe levels of the plastid-encoded RbcL (large subunit of RUBISCO ... investigated ... by western blot analysis ... accumulated in ... soldat10
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed Articlerug2-1 mutant ... DNA microarray ... rpS3 ... downregulated
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed Articlein our microarray ... upregulated ... in rug2-1 ... rpL2
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed Articlein our microarray ... upregulated ... in rug2-1 ... rpS3
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed Articlein our microarray ... upregulated ... in rug2-1 ... C2
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed ArticlepsbA (PSII core complex D1 ... investigated ... western blot analysis ... accumulated in rug2-1
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed Articlerug2-1 mutant ... DNA microarray ... orf25 ... downregulated
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed Articlerug2-1 mutant ... DNA microarray ... ccb206 ... downregulated
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed Articlein our microarray ... upregulated ... in rug2-1 ... rpS15
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed Articlerug2-1 mutant ... DNA microarray ... AtMg00520 ... downregulated
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed ArticleatpB (ATP synthase subunit ... investigated ... by western blot analysis ... accumulated in ... soldat10
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed ArticlepsbA (PSII core complex D1 ... investigated ... by western blot analysis ... accumulated in ... soldat10
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed Articlein our microarray ... upregulated ... rug2-1 ... rpL20
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed Articlerug2-1 mutant ... DNA microarray ... orf294 ... downregulated
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed ArticleThe levels of the plastid-encoded RbcL (large subunit of RUBISCO ... investigated ... by western blot analysis ... accumulated in rug2-1
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed Articlerug2-1 mutant ... DNA microarray ... orf113 ... downregulated
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed ArticleqRT-PCR ... confirmed that in rug2-1 ... cox1 (encoding cytochrome oxidase subunit 1 ... downregulated ... 1.2
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed Articlein our microarray ... upregulated ... in rug2-1 ... psbL
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed Articlein our microarray ... upregulated ... in rug2-1 ... ndhE
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed Articlerug2-1 mutant ... DNA microarray ... mitochondrial genes ... AtMg00500 ... downregulated
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed ArticleWe studied the spatial and temporal expression patterns of RUG2 in Col-0 by qRT-PCR, and detected RUG2 transcripts in all organs and stages analyzed (Figure S4 ... detected in ... stems
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed Articleorf121a ... upregulated in rug2-1
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed Articlein our microarray ... upregulated ... in rug2-1 ... ycf1.2
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed Articlerug2-1 mutant ... DNA microarray ... rpL5 ... downregulated
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed Articlerug2-1 mutant ... DNA microarray ... orf101b ... downregulated
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed Articlein our microarray ... upregulated ... rug2-1 ... rpL16
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed Articlein rug2-1 ... RpoTp/SCA3 ... was upregulated 1.8-fold compared with Ler (1.8 ± 0.3; P = 0.002 ... was upregulated 1
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed ArticleqRT-PCR ... confirmed that in rug2-1 ... psbA ... downregulated ... 1.3-fold
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed ArticleFtsH10 protein ... investigated ... by western blot analysis ... FtsH10 protein was detected ... in soldat10
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed ArticleFtsH10 protein ... investigated ... by western blot analysis ... much less FtsH10 protein was detected in rug2-1
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed Articleorf109b ... upregulated in rug2-1
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed Articlerug2-1 mutant ... DNA microarray ... AtMg00590 ... downregulated
Quesada V, Sarmiento-Mañús R, González-Bayón R, Hricová A, Pérez-Marcos R, Graciá-Martínez E, Medina-Ruiz L, Leyva-Díaz E, Ponce MR, Micol JL - Arabidopsis RUGOSA2 encodes an mTERF family member required for mitochondrion, chloroplast and leaf development
Visit the PubMed ArticleWe then examined the response of these punctate organelles to brefeldin A (BFA), a chemical that affects the distribution of plant organelles including the Golgi and trans-Golgi network (TGN) in Arabidopsis cells (Grebe et al., 2003; Dettmer et al., 2006). When ANp::AN-GFP plants were treated with 100 μm BFA for 30 min, compartments bearing AN-GFP were trapped in aggregated BFA bodies (Figure 5d), whereas large bright dots were not affected. These results again suggested that the large dot-like structures do not reflect physiological localization (this idea was confirmed by immuno-EM as described later). Thus, we hereafter focused our attention on the small punctate structures with weak GFP fluorescence
Minamisawa N, Sato M, Cho KH, Ueno H, Takechi K, Kajikawa M, Yamato KT, Ohyama K, Toyooka K, Kim GT, Horiguchi G, Takano H, Ueda T, Tsukaya H - ANGUSTIFOLIA, a plant homolog of CtBP/BARS, functions outside the nucleus
Visit the PubMed ArticleTo confirm that this localization of AN-GFP around the TGN represented authentic localization of the endogenous AN protein, we then performed immuno-electron microscopy using anti-AN antibody with samples prepared by a freeze-fixation and freeze-substitution method and found that AN actually localized on subdomains of TGN under an electron microscope (Figure 7a, Figure S2). No large dot-like structure was detected
Minamisawa N, Sato M, Cho KH, Ueno H, Takechi K, Kajikawa M, Yamato KT, Ohyama K, Toyooka K, Kim GT, Horiguchi G, Takano H, Ueda T, Tsukaya H - ANGUSTIFOLIA, a plant homolog of CtBP/BARS, functions outside the nucleus
Visit the PubMed ArticleSubcellular localization of AN suggested that AN could function in membrane trafficking around the TGN. We examined the structure and movement of VHA-a1-mRFP-positive compartments in the an-1 mutant for any abnormality. However, we did not detect any difference between an-1 and WT by confocal microscopy (Figure 7d,e). On the other hand, using a transmission electron microscope, we found that vesicles near the TGN in the an-1 mutant were larger compared with those of WT (Figure 7b,c), and the number of snowman-shaped vesicles around the TGN was significantly increased; 8.7% of vesicles exhibited the snowman-shape in an-1 whereas 1.3% of vesicles were snowman-shaped in WT (n = 173 vesicles for an-1 and n = 156 vesicles for WT, P < 0.05, Student’s t-test). These results further provide support for the idea that AN acts in a membrane trafficking event around the TGN
Minamisawa N, Sato M, Cho KH, Ueno H, Takechi K, Kajikawa M, Yamato KT, Ohyama K, Toyooka K, Kim GT, Horiguchi G, Takano H, Ueda T, Tsukaya H - ANGUSTIFOLIA, a plant homolog of CtBP/BARS, functions outside the nucleus
Visit the PubMed ArticleTo investigate the physiological intracellular localization of AN in detail, we then observed the AN-GFP signal using confocal laser scanning microscopy. Observation of ANp::AN-GFP plants under a Nipkow spinning disc microscope revealed that AN-GFP was also localized on many small punctate structures (Figure 5c, arrowheads) with much weaker fluorescence. Weak and diffusive fluorescence was also observed in the cytosol, probably reflecting shuttling of AN, which does not harbor a signal peptide or transmembrane domains in the polypeptide, between the punctate structures and the cytosol
Minamisawa N, Sato M, Cho KH, Ueno H, Takechi K, Kajikawa M, Yamato KT, Ohyama K, Toyooka K, Kim GT, Horiguchi G, Takano H, Ueda T, Tsukaya H - ANGUSTIFOLIA, a plant homolog of CtBP/BARS, functions outside the nucleus
Visit the PubMed ArticleTo understand the precise localization of AN, we crossed ANp::AN-GFP or 35S::AN-GFP with transgenic plants expressing xylosyltransferase (XYLT)-Venus (Pagny et al., 2003), sialyltransferase (ST)-Venus (Boevink et al., 1998), or vacuolar proton ATPase a1 (VHA-a1)-mRFP (Dettmer et al., 2006). These proteins are known as specific markers for the medial-Golgi, trans-Golgi, and TGN, respectively, which are all known to aggregate into BFA bodies upon BFA treatment. 35S::AN-GFP lines were constructed for this experiment because intensity of GFP signal of AN-GFP in ANp::AN-GFP plants was not stable, probably due to multiple copies of the transgene. Fluorescence intensity of the 35S::AN-GFP transgenic lines with a single T-DNA insertion was strong, and confirmed that 35S::AN-GFP shows the same intracellular localization as ANp::AN-GFP and completely rescued the an-1 phenotypes (data not shown). Using these plants, we demonstrated that AN-GFP localized on small punctate structures close to the trans-Golgi cisternae labeled by ST-Venus, but their localization did not overlap (Figure 6a). When co-expressed with XYLT-Venus, AN-GFP similarly localized to punctate structures (Figure 6b), but the distance between medial cisternae labeled by XYLT-Venus and AN-GFP compartments seemed to be larger than that between ST-Venus and AN-GFP (compare Figure 6a and b). Next, we observed plants coexpressing AN-GFP and VHA-a1-mRFP and found that these partially colocalized (Figure 6c
Minamisawa N, Sato M, Cho KH, Ueno H, Takechi K, Kajikawa M, Yamato KT, Ohyama K, Toyooka K, Kim GT, Horiguchi G, Takano H, Ueda T, Tsukaya H - ANGUSTIFOLIA, a plant homolog of CtBP/BARS, functions outside the nucleus
Visit the PubMed ArticleWe first observed the localization pattern of AN-GFP in the ANp::AN-GFP line mentioned above using an epifluorescent microscope, which revealed that AN-GFP was localized to large dot-like structures and the cytosol as observed in transgenic plants expressing AN-GFP under regulation of 35S promoter (Kim et al., 2002). The size of the dot-like structures seemed to be comparable to the size of the nucleus, which was why we concluded nuclear localization of AN-GFP in the previous study. To verify the nuclear localization, we examined whether large dots of AN-GFP was stained with DAPI, to find that the AN-GFP dots do not overlap with fluorescence from DAPI either in trichomes or epidermal cells (Figure 5a,b). In addition to the DAPI staining, we also examined whether several organelle markers were localized to the large dot-like structures, but no organelle marker examined (described below) overlapped with these structures. These results, together with extremely large size and bright fluorescence, suggested that the large dot-like structures could represent non-physiological aggregates
Minamisawa N, Sato M, Cho KH, Ueno H, Takechi K, Kajikawa M, Yamato KT, Ohyama K, Toyooka K, Kim GT, Horiguchi G, Takano H, Ueda T, Tsukaya H - ANGUSTIFOLIA, a plant homolog of CtBP/BARS, functions outside the nucleus
Visit the PubMed ArticleTo investigate the spatial and temporal patterns of CYCB1;1 affected by NEK6, the CYCB1;1:GUS fusion gene was used as a marker to examine cells in G2/M phase ... The transgenic reporter plant that expresses CYCB1;1;GUS ... was crossed with the nek6 mutant ... and the resulting progenies were tested for GUS activity ... Compared with reporter line Col, nek6 showed less GUS activity in shoot apexes
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleWe examined if the ethylene biosynthesis-related genes and ethylene signaling-related genes were altered in the nek6 mutant ... ACS7 ... clearly up-regulated in the nek6 mutant
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleGUS staining, representing the NEK6 expression level, was found ... in ... petioles
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleIn the two NEK6-overexpressing lines, expression of cyclin genes ... CYCA3;1 was enhanced significantly compared with Col
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleWe examined if the ethylene biosynthesis-related genes and ethylene signaling-related genes were altered in the ... NEK6-overexpressing plants ... ACO4 ... expression tended to decrease in the NEK6-overexpressing lines compared with that in Col
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleWe examined if the ethylene biosynthesis-related genes and ethylene signaling-related genes were altered in the ... NEK6-overexpressing plants ... ACS5 ... expression tended to decrease in the NEK6-overexpressing lines compared with that in Col
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleWe examined if the ethylene biosynthesis-related genes and ethylene signaling-related genes were altered in the ... NEK6-overexpressing plants ... EBP ... found at substantially lower levels in the NEK6-overexpressing lines
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleWe examined if the ethylene biosynthesis-related genes and ethylene signaling-related genes were altered in the ... NEK6-overexpressing plants ... EIL1 ... found at substantially lower levels in the NEK6-overexpressing lines
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleWe examined if the ethylene biosynthesis-related genes and ethylene signaling-related genes were altered in the ... NEK6-overexpressing plants ... ERF4 ... found at substantially lower levels in the NEK6-overexpressing lines
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleWe examined if the ethylene biosynthesis-related genes and ethylene signaling-related genes were altered in the ... NEK6-overexpressing plants ... ACS7 ... expression tended to decrease in the NEK6-overexpressing lines compared with that in Col
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleNEK6 expression ... in 10-day-old Arabidopsis seedlings ... was ... induced to a stably moderate level throughout 200 mm NaCl treatment
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleTo investigate the spatial and temporal patterns of CYCB1;1 affected by NEK6, the CYCB1;1:GUS fusion gene was used as a marker to examine cells in G2/M phase ... The transgenic reporter plant that expresses CYCB1;1;GUS ... was crossed with the ... NEK6-overexpressing lines, and the resulting progenies were tested for GUS activity ... The crossed OE2 ... still had higher ... NEK6 expression ... compared with Col
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleWe examined if the ethylene biosynthesis-related genes and ethylene signaling-related genes were altered in the nek6 mutant ... Ethylene signaling-related genes, including the ethylene receptor gene ERS1 ... were also expressed at higher levels in the nek6 mutant
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleNEK6, under the control of CaMV 35S promoter plus a translational enhancer Ω, was transferred into Arabidopsis plants ... overexpressing lines ... had higher gene expressions by northern blot ... compared with Col
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleWe examined if the ethylene biosynthesis-related genes and ethylene signaling-related genes were altered in the nek6 mutant ... ACS1 ... clearly up-regulated in the nek6 mutant
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleWe examined if the ethylene biosynthesis-related genes and ethylene signaling-related genes were altered in the ... NEK6-overexpressing plants ... ERF1 ... found at substantially lower levels in the NEK6-overexpressing lines
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleWe examined if the ethylene biosynthesis-related genes and ethylene signaling-related genes were altered in the ... NEK6-overexpressing plants ... ACS2 ... expression tended to decrease in the NEK6-overexpressing lines compared with that in Col
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleGUS staining, representing the NEK6 expression level, was found ... in ... young leaves
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed Articlewe examined if this gene expression could be altered by ethylene precursor ACC ... After immersion in 10 μm ACC, NEK6 expression was clearly induced to a peak level at 6 h after initiation of the treatment in 10-day-old Arabidopsis seedlings
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleTo investigate the spatial and temporal patterns of CYCB1;1 affected by NEK6, the CYCB1;1:GUS fusion gene was used as a marker to examine cells in G2/M phase ... The transgenic reporter plant that expresses CYCB1;1;GUS ... was crossed with the ... NEK6-overexpressing lines, and the resulting progenies were tested for GUS activity ... Compared with reporter line Col ... in the NEK6-overexpressing line OE2, GUS activity was substantially higher in ... petioles
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleWe examined if the ethylene biosynthesis-related genes and ethylene signaling-related genes were altered in the nek6 mutant ... ACS5 ... clearly up-regulated in the nek6 mutant
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleWe examined if the ethylene biosynthesis-related genes and ethylene signaling-related genes were altered in the nek6 mutant ... ACO2 ... clearly up-regulated in the nek6 mutant
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleWe examined if the ethylene biosynthesis-related genes and ethylene signaling-related genes were altered in the ... NEK6-overexpressing plants ... ERS1 ... found at substantially lower levels in the NEK6-overexpressing lines
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleTo investigate the spatial and temporal patterns of CYCB1;1 affected by NEK6, the CYCB1;1:GUS fusion gene was used as a marker to examine cells in G2/M phase ... The transgenic reporter plant that expresses CYCB1;1;GUS ... was crossed with ... NEK6-overexpressing lines, and the resulting progenies were tested for GUS activity ... Compared with reporter line Col ... in the NEK6-overexpressing line OE2, GUS activity was substantially higher in shoot apexes
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleNEK6, under the control of CaMV 35S promoter plus a translational enhancer Ω, was transferred into Arabidopsis plants ... overexpressing lines ... had higher gene expressions by ... quantitative PCR ... compared with Col
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleWe examined if the ethylene biosynthesis-related genes and ethylene signaling-related genes were altered in the nek6 mutant ... ACS2 ... clearly up-regulated in the nek6 mutant
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleWe examined if the ethylene biosynthesis-related genes and ethylene signaling-related genes were altered in the nek6 mutant ... EIN3-like transcription factor gene EIL1 ... were also expressed at higher levels in the nek6 mutant
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleWe examined if the ethylene biosynthesis-related genes and ethylene signaling-related genes were altered in the nek6 mutant ... ERF4 ... were also expressed at higher levels in the nek6 mutant
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleNEK6, under the control of CaMV 35S promoter plus a translational enhancer Ω, was transferred into Arabidopsis plants ... overexpressing lines ... had higher protein levels compared with Col
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleTo investigate the spatial and temporal patterns of CYCB1;1 affected by NEK6, the CYCB1;1:GUS fusion gene was used as a marker to examine cells in G2/M phase ... The transgenic reporter plant that expresses CYCB1;1;GUS ... was crossed with the nek6 mutant ... and the resulting progenies were tested for GUS activity. The crossed ... NEK6 still had ... lower NEK6 expression ... compared with Col
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleA nek6 mutant harboring a T-DNA insertion between exon 7 and exon 8 ... showed no NEK6 gene expression
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleWe examined if the ethylene biosynthesis-related genes and ethylene signaling-related genes were altered in the ... NEK6-overexpressing plants ... ACS1 ... expression tended to decrease in the NEK6-overexpressing lines compared with that in Col
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleWe examined if the ethylene biosynthesis-related genes and ethylene signaling-related genes were altered in the nek6 mutant ... ACO4 ... clearly up-regulated in the nek6 mutant
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleIn the two NEK6-overexpressing lines, expression of cyclin genes CYCB1;1 ... was enhanced significantly compared with Col
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleWe examined if the ethylene biosynthesis-related genes and ethylene signaling-related genes were altered in the ... NEK6-overexpressing plants ... ACO2 ... expression tended to decrease in the NEK6-overexpressing lines compared with that in Col
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleUsing quantitative PCR, we further confirmed that NEK6 transcript levels in ... leaves
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed Articleexpression of cyclin genes ... CYCA3;1 ... was clearly reduced in nek6 mutant
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleTo investigate the spatial and temporal patterns of CYCB1;1 affected by NEK6, the CYCB1;1:GUS fusion gene was used as a marker to examine cells in G2/M phase ... The transgenic reporter plant that expresses CYCB1;1;GUS ... was crossed with ... NEK6-overexpressing lines, and the resulting progenies were tested for GUS activity ... Compared with reporter line Col ... in the NEK6-overexpressing line OE2, GUS activity was substantially higher in ... young leaves
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleGUS staining, representing the NEK6 expression level, was found ... In mature rosette leaves of pot-grown plants ... expressed weakly in vascular tissues
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleWe examined if the ethylene biosynthesis-related genes and ethylene signaling-related genes were altered in the nek6 mutant ... ERF1 ... were also expressed at higher levels in the nek6 mutant
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleWe examined if the ethylene biosynthesis-related genes and ethylene signaling-related genes were altered in the nek6 mutant ... EBP ... were also expressed at higher levels in the nek6 mutant
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed Articleexpression of cyclin genes CYCB1;1 ... was clearly reduced in nek6 mutant
Zhang B, Chen HW, Mu RL, Zhang WK, Zhao MY, Wei W, Wang F, Yu H, Lei G, Zou HF, Ma B, Chen SY, Zhang JS - NIMA-related kinase NEK6 affects plant growth and stress response in Arabidopsis
Visit the PubMed ArticleWe have previously shown that the double mutation myb3r1 myb3r4 results in decreased KN mRNA levels (Haga et al., 2007). To reveal spatial aspects of the activation of KN transcription mediated by the MSA-MYB pathway, we examined the expression of a KN promoter-GUS fusion construct (KN::GUS) in the wild type and the double mutants ... GUS activity was also detected in young leaves, where dividing stomatal lineage cells, such as meristemoids and guard mother cells, were densely stained (Fig. 5B). The GUS activity in these cells was markedly decreased in the myb3r1 myb3r4 double mutant
Haga N, Kobayashi K, Suzuki T, Maeo K, Kubo M, Ohtani M, Mitsuda N, Demura T, Nakamura K, Jürgens G, Ito M - Mutations in MYB3R1 and MYB3R4 cause pleiotropic developmental defects and preferential down-regulation of multiple G2/M-specific genes in Arabidopsis
Visit the PubMed ArticleWe also observed that the KN promoter had very weak activity in the meristematic region of the root and dividing stomatal lineage cells when all the MSA motifs were mutated
Haga N, Kobayashi K, Suzuki T, Maeo K, Kubo M, Ohtani M, Mitsuda N, Demura T, Nakamura K, Jürgens G, Ito M - Mutations in MYB3R1 and MYB3R4 cause pleiotropic developmental defects and preferential down-regulation of multiple G2/M-specific genes in Arabidopsis
Visit the PubMed ArticleTo evaluate the effects of the myb3r1 myb3r4 double mutation on the expression of G2/M-specific genes, we first defined 185 genes with G2/M-specific expression by a combination of coexpression analysis using ATTED-II (Obayashi et al., 2009) and gene clustering analysis with the microarray data of synchronized MM2d cells (Menges et al., 2003; see “Materials and Methods”). This newly defined G2/M-specific class contained 79 out of 82 genes that had been previously regarded as mitosis-specific genes (Menges et al., 2005) and 134 out of 352 genes that had been classified as M-associated genes (Menges et al., 2003; Supplemental Table S3). When applied to our microarray data, we noticed a strong tendency for this new class of genes to be frequently and strongly down-regulated in the double mutant. Figure 6 displays a frequency distribution of genes against the fold change in the expression levels between the double mutant and the wild type. It was obvious that the frequency distribution of G2/M-specific genes was strongly biased toward down-regulation in the double mutant when compared with the distribution patterns of all other genes on the microarray. Approximately 11.6% of G2/M-specific genes were down-regulated more than 2-fold in the double mutant, whereas only 0.38% of all 16,034 genes analyzed showed such down-regulation. Table II shows the top 30 genes that are most down-regulated in the double mutant (P < 0.05, paired t test), which included 16 genes (53.3%) of the G2/M-specific class, although this class represents only 0.83% of all genes on the microarray. These results supported the idea that MYB3R1 and MYB3R4 may transcriptionally activate multiple G2/M phase-specific genes in Arabidopsis. However, this analysis also revealed that not all the G2/M-specific genes were affected by the double mutation. In fact, the majority of G2/M-specific genes were unaffected or even up-regulated in the myb3r1 myb3r4 double mutant (Fig. 6). In addition, many genes whose expression is not related to the cell cycle were also significantly down-regulated in the double mutant, although roughly as many genes were up-regulated to a similar extent
Haga N, Kobayashi K, Suzuki T, Maeo K, Kubo M, Ohtani M, Mitsuda N, Demura T, Nakamura K, Jürgens G, Ito M - Mutations in MYB3R1 and MYB3R4 cause pleiotropic developmental defects and preferential down-regulation of multiple G2/M-specific genes in Arabidopsis
Visit the PubMed Articlethe SND1 promoter was activated more than 18-fold when protoplasts were co-transfected with 35S:SND1, but this trans-activation activity was reduced to around twofold for the T94K mutation
Wang H, Zhao Q, Chen F, Wang M, Dixon RA - NAC domain function and transcriptional control of a secondary cell wall master switch
Visit the PubMed ArticleOverexpression of MYB32 and the corresponding repression of SND1 expression were observed in stem tissues of two independent transgenic lines, as determined by quantitative reverse transcription (qRT)-PCR
Wang H, Zhao Q, Chen F, Wang M, Dixon RA - NAC domain function and transcriptional control of a secondary cell wall master switch
Visit the PubMed ArticleThe wild-type AtSND1 fusion protein was localized to the nucleus as previously reported (Zhong et al., 2006)
Wang H, Zhao Q, Chen F, Wang M, Dixon RA - NAC domain function and transcriptional control of a secondary cell wall master switch
Visit the PubMed ArticleAtOFP12 ... showed ubiquitous expression in all tissues/organs examined
Wang S, Chang Y, Guo J, Zeng Q, Ellis BE, Chen JG - Arabidopsis ovate family proteins, a novel transcriptional repressor family, control multiple aspects of plant growth and development
Visit the PubMed ArticleAtOFP6 ... showed ubiquitous expression in all tissues/organs examined
Wang S, Chang Y, Guo J, Zeng Q, Ellis BE, Chen JG - Arabidopsis ovate family proteins, a novel transcriptional repressor family, control multiple aspects of plant growth and development
Visit the PubMed ArticleAtOFP16 ... showed ubiquitous expression in all tissues/organs examined
Wang S, Chang Y, Guo J, Zeng Q, Ellis BE, Chen JG - Arabidopsis ovate family proteins, a novel transcriptional repressor family, control multiple aspects of plant growth and development
Visit the PubMed Articlewe generated transgenic Arabidopsis plants containing a MED25 promoter::GUS fusion (pMED25::GUS). In seedlings, GUS activity was detected in ... leaves
Xu R, Li Y - Control of final organ size by Mediator complex subunit 25 in Arabidopsis thaliana
Visit the PubMed Articlewe expressed MED25 under the control of the 35S promoter in the Col-0 wild type ... Transgenic plants had significant increases in MED25 mRNA compared with wild-type plants
Xu R, Li Y - Control of final organ size by Mediator complex subunit 25 in Arabidopsis thaliana
Visit the PubMed ArticleTo investigate expression of MED25, we performed quantitative RT-PCR analysis. MED25 mRNA was detected in ... stems
Xu R, Li Y - Control of final organ size by Mediator complex subunit 25 in Arabidopsis thaliana
Visit the PubMed ArticleTo investigate expression of MED25, we performed quantitative RT-PCR analysis. MED25 mRNA was detected in ... leaves
Xu R, Li Y - Control of final organ size by Mediator complex subunit 25 in Arabidopsis thaliana
Visit the PubMed ArticleTo investigate expression of MED25, we performed quantitative RT-PCR analysis. MED25 mRNA was detected in ... seedlings
Xu R, Li Y - Control of final organ size by Mediator complex subunit 25 in Arabidopsis thaliana
Visit the PubMed Articlewe generated transgenic Arabidopsis plants containing a MED25 promoter::GUS fusion (pMED25::GUS). In seedlings, GUS activity was detected in cotyledons
Xu R, Li Y - Control of final organ size by Mediator complex subunit 25 in Arabidopsis thaliana
Visit the PubMed Articlewe analyzed AS1 expression under continuous light (LL) conditions. The AS1 mRNA level oscillated diurnally under LL conditions (Figure 2e), indicating that AS1 expression is regulated by the circadian clock. Together with the spatial expression pattern of AS1, our data indicated that AS1 is largely expressed in the same spatiotemporal domain as CO
Song YH, Lee I, Lee SY, Imaizumi T, Hong JC - CONSTANS and ASYMMETRIC LEAVES 1 complex is involved in the induction of FLOWERING LOCUS T in photoperiodic flowering in Arabidopsis.
Visit the PubMed ArticleFor this experiment, the as1 mutation was introduced into SUC2:CO–HA plants by genetic cross, and we confirmed that CO expression levels in the transgenic plants with or without the as1 mutation were similar (Figure 5d). In the SUC2:CO–HA plants, the FT expression level was drastically higher than that in wild-type plants in LD and 12L/12D (Figure 5e,f). In the SUC:CO–HA/as1-21 line, the FT expression level was still higher than that in the wild type; however, it was markedly lower than that in the SUC2:CO–HA plants (Figure 5e,f). This result suggests that the elevated CO levels (=sensitized conditions) facilitate the depiction of the AS1 contribution to CO-dependent FT expression
Song YH, Lee I, Lee SY, Imaizumi T, Hong JC - CONSTANS and ASYMMETRIC LEAVES 1 complex is involved in the induction of FLOWERING LOCUS T in photoperiodic flowering in Arabidopsis.
Visit the PubMed ArticleTo study the spatial expression pattern of AS1, we analyzed the GUS expression pattern derived from the AS1 genomic fragment fused to the GUS gene (AS1:AS1–GUS). The GUS activity was predominantly found in the phloem tissues of ... cotyledons ... The expression patterns were similar within plants grown under various growth conditions (Figure S2)
Song YH, Lee I, Lee SY, Imaizumi T, Hong JC - CONSTANS and ASYMMETRIC LEAVES 1 complex is involved in the induction of FLOWERING LOCUS T in photoperiodic flowering in Arabidopsis.
Visit the PubMed ArticleTo study the spatial expression pattern of AS1, we analyzed the GUS expression pattern derived from the AS1 genomic fragment fused to the GUS gene (AS1:AS1–GUS). The GUS activity was predominantly found in the phloem tissues of leaves ... The expression patterns were similar within plants grown under various growth conditions (Figure S2)
Song YH, Lee I, Lee SY, Imaizumi T, Hong JC - CONSTANS and ASYMMETRIC LEAVES 1 complex is involved in the induction of FLOWERING LOCUS T in photoperiodic flowering in Arabidopsis.
Visit the PubMed ArticleWe then analyzed the temporal expression patterns of AS1 under LD and SD conditions. Under these conditions, AS1 mRNA levels changed throughout the day with an afternoon peak in LD and with a broad night peak in SD (Figure 2c,d). In LD, the AS1 peak coincides with the daytime peak of CO (Suarez-Lopez et al., 2001)
Song YH, Lee I, Lee SY, Imaizumi T, Hong JC - CONSTANS and ASYMMETRIC LEAVES 1 complex is involved in the induction of FLOWERING LOCUS T in photoperiodic flowering in Arabidopsis.
Visit the PubMed ArticleIn addition, when AS1–GFP and CO–RFP were co-expressed in Arabidopsis protoplasts, both GFP and RFP fluorescence co-localized in the nucleus (Figure 2a), indicating that both CO and AS1 exist in the same domain in the nucleus
Song YH, Lee I, Lee SY, Imaizumi T, Hong JC - CONSTANS and ASYMMETRIC LEAVES 1 complex is involved in the induction of FLOWERING LOCUS T in photoperiodic flowering in Arabidopsis.
Visit the PubMed Articleaddition, when AS1–GFP and CO–RFP were co-expressed in Arabidopsis protoplasts, both GFP and RFP fluorescence co-localized in the nucleus (Figure 2a), indicating that both CO and AS1 exist in the same domain in the nucleus
Song YH, Lee I, Lee SY, Imaizumi T, Hong JC - CONSTANS and ASYMMETRIC LEAVES 1 complex is involved in the induction of FLOWERING LOCUS T in photoperiodic flowering in Arabidopsis.
Visit the PubMed ArticleThe FT transcript levels in the as1 mutant were slightly lower than those in the wild-type plants in LD and 12L/12D
Song YH, Lee I, Lee SY, Imaizumi T, Hong JC - CONSTANS and ASYMMETRIC LEAVES 1 complex is involved in the induction of FLOWERING LOCUS T in photoperiodic flowering in Arabidopsis.
Visit the PubMed Articlethe loss of as1 function indirectly reduces AtGA20ox1 expression
Song YH, Lee I, Lee SY, Imaizumi T, Hong JC - CONSTANS and ASYMMETRIC LEAVES 1 complex is involved in the induction of FLOWERING LOCUS T in photoperiodic flowering in Arabidopsis.
Visit the PubMed ArticlePHT4;2 expression was ... detected in roots but not in leaves
Irigoyen S, Karlsson PM, Kuruvilla J, Spetea C, Versaw WK - The sink-specific plastidic phosphate transporter PHT4;2 influences starch accumulation and leaf size in Arabidopsis
Visit the PubMed Articleas2-1 rev-1 ... when ... treated with 1 μM NPA ... we did not observe the small GUS-staining spots on leaf surfaces
Wang W, Xu B, Wang H, Li J, Huang H, Xu L - YUCCA genes are expressed in response to leaf adaxial-abaxial juxtaposition and are required for leaf margin development
Visit the PubMed ArticlepDR5::GUS ... staining in the ... kan1-11 kan2-5 leaves was also concentrated in the top portion of the protrusions
Wang W, Xu B, Wang H, Li J, Huang H, Xu L - YUCCA genes are expressed in response to leaf adaxial-abaxial juxtaposition and are required for leaf margin development
Visit the PubMed ArticleYUC4 was also strongly expressed in vascular tissue connecting the leaf tip and hydathodes
Wang W, Xu B, Wang H, Li J, Huang H, Xu L - YUCCA genes are expressed in response to leaf adaxial-abaxial juxtaposition and are required for leaf margin development
Visit the PubMed Articleas2-1 rev-1 ... expressions of ... YUC4 ... associated with the protrusions on leaves
Wang W, Xu B, Wang H, Li J, Huang H, Xu L - YUCCA genes are expressed in response to leaf adaxial-abaxial juxtaposition and are required for leaf margin development
Visit the PubMed ArticleYUC2 was also preferentially expressed in the central-layer cells of a hydathode
Wang W, Xu B, Wang H, Li J, Huang H, Xu L - YUCCA genes are expressed in response to leaf adaxial-abaxial juxtaposition and are required for leaf margin development
Visit the PubMed Articleas2-1 rev-1 ... expressions of YUC1 ... associated with the protrusions on leaves
Wang W, Xu B, Wang H, Li J, Huang H, Xu L - YUCCA genes are expressed in response to leaf adaxial-abaxial juxtaposition and are required for leaf margin development
Visit the PubMed ArticleYUC1 was expressed in the entire leaf primordium
Wang W, Xu B, Wang H, Li J, Huang H, Xu L - YUCCA genes are expressed in response to leaf adaxial-abaxial juxtaposition and are required for leaf margin development
Visit the PubMed ArticleWeak YUC2 staining was detected throughout the entire leaf primordium
Wang W, Xu B, Wang H, Li J, Huang H, Xu L - YUCCA genes are expressed in response to leaf adaxial-abaxial juxtaposition and are required for leaf margin development
Visit the PubMed ArticleYUC4 ... As the leaf developed, GUS staining gradually intensified in hydathodes
Wang W, Xu B, Wang H, Li J, Huang H, Xu L - YUCCA genes are expressed in response to leaf adaxial-abaxial juxtaposition and are required for leaf margin development
Visit the PubMed ArticleYUC1 staining was also observed immediately distal to a hydathode
Wang W, Xu B, Wang H, Li J, Huang H, Xu L - YUCCA genes are expressed in response to leaf adaxial-abaxial juxtaposition and are required for leaf margin development
Visit the PubMed ArticleExpression of YUC4 was not detected in young leaf primordia
Wang W, Xu B, Wang H, Li J, Huang H, Xu L - YUCCA genes are expressed in response to leaf adaxial-abaxial juxtaposition and are required for leaf margin development
Visit the PubMed ArticleYUC1 staining was only detected ... the petiole of 8-DAG leaves
Wang W, Xu B, Wang H, Li J, Huang H, Xu L - YUCCA genes are expressed in response to leaf adaxial-abaxial juxtaposition and are required for leaf margin development
Visit the PubMed ArticleYUC2 ... relatively strong GUS staining was observed in the meristem
Wang W, Xu B, Wang H, Li J, Huang H, Xu L - YUCCA genes are expressed in response to leaf adaxial-abaxial juxtaposition and are required for leaf margin development
Visit the PubMed Articleas2-1 rev-1 ... expressions of ... YUC2 ... associated with the protrusions on leaves
Wang W, Xu B, Wang H, Li J, Huang H, Xu L - YUCCA genes are expressed in response to leaf adaxial-abaxial juxtaposition and are required for leaf margin development
Visit the PubMed Articlekan1-11 kan2-5 ... expressions of YUC1 ... associated with the protrusions on leaves
Wang W, Xu B, Wang H, Li J, Huang H, Xu L - YUCCA genes are expressed in response to leaf adaxial-abaxial juxtaposition and are required for leaf margin development
Visit the PubMed ArticlepDR5::GUS ... staining in the as2-1 rev-1 ... leaves was also concentrated in the top portion of the protrusions
Wang W, Xu B, Wang H, Li J, Huang H, Xu L - YUCCA genes are expressed in response to leaf adaxial-abaxial juxtaposition and are required for leaf margin development
Visit the PubMed ArticleYUC4 was expressed in the leaf protrusions of the mock-treated as2-1 rev-1
Wang W, Xu B, Wang H, Li J, Huang H, Xu L - YUCCA genes are expressed in response to leaf adaxial-abaxial juxtaposition and are required for leaf margin development
Visit the PubMed Articlekan1-11 kan2-5 ... expressions of ... YUC2 ... associated with the protrusions on leaves
Wang W, Xu B, Wang H, Li J, Huang H, Xu L - YUCCA genes are expressed in response to leaf adaxial-abaxial juxtaposition and are required for leaf margin development
Visit the PubMed Articlekan1-11 kan2-5 ... expressions of ... YUC4 ... associated with the protrusions on leaves
Wang W, Xu B, Wang H, Li J, Huang H, Xu L - YUCCA genes are expressed in response to leaf adaxial-abaxial juxtaposition and are required for leaf margin development
Visit the PubMed ArticleYUC1 staining was only detected in the proximal margin ... of 8-DAG leaves
Wang W, Xu B, Wang H, Li J, Huang H, Xu L - YUCCA genes are expressed in response to leaf adaxial-abaxial juxtaposition and are required for leaf margin development
Visit the PubMed ArticleYUC1 ... in the ... leaf ... strongest GUS staining was found in the proximal part
Wang W, Xu B, Wang H, Li J, Huang H, Xu L - YUCCA genes are expressed in response to leaf adaxial-abaxial juxtaposition and are required for leaf margin development
Visit the PubMed Articlekan1-11 kan2-5 ... when ... treated with 1 μM NPA ... we did not observe the small GUS-staining spots on leaf surfaces
Wang W, Xu B, Wang H, Li J, Huang H, Xu L - YUCCA genes are expressed in response to leaf adaxial-abaxial juxtaposition and are required for leaf margin development
Visit the PubMed ArticleYUC4 was expressed in the leaf protrusions of the mock-treated ... kan1-11 kan2-5
Wang W, Xu B, Wang H, Li J, Huang H, Xu L - YUCCA genes are expressed in response to leaf adaxial-abaxial juxtaposition and are required for leaf margin development
Visit the PubMed ArticleThe RNA gel blot analyses show that SAG113 is induced by treatments with ABA for 1 h and 3 h
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Visit the PubMed Articlethe SAG113 expression increased with the progression of leaf senescence in the wild type ... and abi4-2 ... However, the transcript levels of SAG113 in ... abi4-2 ... were significantly reduced compared with those of the wild type
Zhang K, Xia X, Zhang Y, Gan SS - An ABA-regulated and Golgi-localized protein phosphatase controls water loss during leaf senescence in Arabidopsis
Visit the PubMed ArticleTo determine the subcellular localization of SAG113, the SAG113 full coding region was first fused with GFP and transferred into wild-type plants and the epidermis peels containing guard cells examined using confocal microscopy. The GFP signal was observed in numerous small subcellular vesicles in the guard cells (cf. Figure 6b), indicating that SAG113 may be localized to the Golgi apparatus and/or mitochondria. A known Golgi marker, ERH1-DsRed (Wang et al., 2008), was then transferred into the SAG113-GFP transgenic background and imaged using the DsRED channel setting of the confocal microscope (Figure 6c). The Golgi marker co-localized with the SAG113-GFP (Figures 6b and S1) when the same cells were examined using the GFP channel setting. In our settings, the GFP signal (from a 35S-GFP control plant) could be imaged using the GFP channel only (Figure 6f) but not the DsRED channel (Figure 6g). ERH1-DsRed (red fluorescence) and SAG113-GFP (green fluorescence) were also found to be co-localized in root cells using the same channel settings as above (Figure S2). These data strongly suggest that SAG113 is localized in the Golgi apparatus
Zhang K, Xia X, Zhang Y, Gan SS - An ABA-regulated and Golgi-localized protein phosphatase controls water loss during leaf senescence in Arabidopsis
Visit the PubMed Articlethe SAG113 expression increased with the progression of leaf senescence in the wild type, aba2-1 ... However, the transcript levels of SAG113 in ... aba2-1 ... were significantly reduced compared with those of the wild type
Zhang K, Xia X, Zhang Y, Gan SS - An ABA-regulated and Golgi-localized protein phosphatase controls water loss during leaf senescence in Arabidopsis
Visit the PubMed ArticleThe expression pattern of SAG113 in response to ABA was also confirmed by GUS staining of young leaves following ABA treatment. The young leaves treated with ABA showed uniform and strong GUS staining (right panel in Figure 2b) while the control treatment did not display any GUS staining (left panel in Figure 2b). When the epidermal peels were further examined by microscopy, the GUS expression was found in guard cells
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Visit the PubMed ArticleThe RNA gel blot analysis indicated that the expression of the typical senescence marker ... SAG12 ... was strongly up-regulated in the SAG113-induced transgenic plants
Zhang K, Xia X, Zhang Y, Gan SS - An ABA-regulated and Golgi-localized protein phosphatase controls water loss during leaf senescence in Arabidopsis
Visit the PubMed ArticleTo further investigate the expression pattern of SAG113, its 2.2-kb promoter region was fused with the GUS reporter gene ... When the epidermal peels of the stained senescing leaves were examined under a light microscope in detail, the GUS expression was found in guard cells
Zhang K, Xia X, Zhang Y, Gan SS - An ABA-regulated and Golgi-localized protein phosphatase controls water loss during leaf senescence in Arabidopsis
Visit the PubMed ArticleThe expression of SAG113 was examined in expanding young leaves, fully expanded mature leaves, early senescence stage leaves and late senescence stage leaves using RNA gel blot analysis. The SAG113 transcripts were detected in leaves at both early and late senescence stages but not in young leaves or mature leaves
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Visit the PubMed ArticleTo further investigate the expression pattern of SAG113, its 2.2-kb promoter region was fused with the GUS reporter gene. The GUS staining pattern of the rosette leaves in transgenic Arabidopsis 40 days after germination (DAG) shows that SAG113 is expressed in senescing leaves
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Visit the PubMed ArticleThe RNA gel blot analysis indicated that the expression of the typical senescence marker ... SAG13 was strongly up-regulated in the SAG113-induced transgenic plants
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Visit the PubMed ArticleQuantitative real-time polymerase chain reaction (qRT-PCR) analyses of DNA repair and replication checkpoint genes in wild-type, elo3, elp5 and drl1 seedlings ... DNA damage accumulation often results in inductions of DNA repair and DNA replication checkpoint genes; BRAC1, RAD51, PARP2, and WEE1 are such genes in Arabidopsis (Kotogany et al., 2010). We thus examined expression of these genes in the Elongator mutants, and found that transcript levels of all these genes were elevated markedly
Xu D, Huang W, Li Y, Wang H, Huang H, Cui X - Elongator complex is critical for cell cycle progression and leaf patterning in Arabidopsis
Visit the PubMed ArticleWe next examined expression of leaf polarity marker genes REVOLUTA (REV) and FILAMENTOUS FLOWER (FIL) in the elo2 as2 background ... the leaf abaxial marker FIL was expressed strongly throughout the needle-like leaves of elo2 as2
Xu D, Huang W, Li Y, Wang H, Huang H, Cui X - Elongator complex is critical for cell cycle progression and leaf patterning in Arabidopsis
Visit the PubMed ArticleQuantitative real-time polymerase chain reaction (qRT-PCR) analyses of DNA repair and replication checkpoint genes in wild-type, elo3, elp5 and drl1 seedlings ... DNA damage accumulation often results in inductions of DNA repair and DNA replication checkpoint genes; BRAC1, RAD51, PARP2, and WEE1 are such genes in Arabidopsis (Kotogany et al., 2010). We thus examined expression of these genes in the Elongator mutants, and found that transcript levels of all these genes were elevated markedly
Xu D, Huang W, Li Y, Wang H, Huang H, Cui X - Elongator complex is critical for cell cycle progression and leaf patterning in Arabidopsis
Visit the PubMed ArticleWe then introduced a pCYCB1;1:Dbox-GUS construct (CYCB1;1:GUS) into wild-type and elo3 plants, as GUS staining in the plants could reflect a cell state of G2 to M transition (Colon-Carmona et al., 1999 ... accumulation of the CYCB1;1:GUS signal in elo3 leaf primordia was much stronger than that in the wild type during early development of leaves, in which cells were undergoing vigorous proliferation
Xu D, Huang W, Li Y, Wang H, Huang H, Cui X - Elongator complex is critical for cell cycle progression and leaf patterning in Arabidopsis
Visit the PubMed ArticleWe then examined transcript levels of several cell cycle-related marker genes by qRT-PCR, using 8-day-old leaves. Cyclin D3;1 (CYCD3;1) is predominantly expressed in the G1-phase and drives the G1/S transition (Dewitte et al., 2003; Menges et al., 2006), H4b is expressed specifically in the S-phase (Soni et al., 1995), while CDT1a, ORC1b, MCM3 and PCNA2 are involved in S-phase DNA replication (Shultz et al., 2007). In addition, CYCA2;1 and CYCB1;1 are involved in S/G2 and G2/M transitions, respectively (Doerner et al., 1996; Shaul et al., 1996). Expression of these markers represents different phases of the cell cycle, and their expression must be downregulated after the corresponding phase of the cell cycle is past. The transcript levels of these tested marker genes were consistently elevated in elo3 leaves (Figure 3e), which suggested that Elongator is required for normal cell-cycle progression
Xu D, Huang W, Li Y, Wang H, Huang H, Cui X - Elongator complex is critical for cell cycle progression and leaf patterning in Arabidopsis
Visit the PubMed ArticleWe then examined transcript levels of several cell cycle-related marker genes by qRT-PCR, using 8-day-old leaves. Cyclin D3;1 (CYCD3;1) is predominantly expressed in the G1-phase and drives the G1/S transition (Dewitte et al., 2003; Menges et al., 2006), H4b is expressed specifically in the S-phase (Soni et al., 1995), while CDT1a, ORC1b, MCM3 and PCNA2 are involved in S-phase DNA replication (Shultz et al., 2007). In addition, CYCA2;1 and CYCB1;1 are involved in S/G2 and G2/M transitions, respectively (Doerner et al., 1996; Shaul et al., 1996). Expression of these markers represents different phases of the cell cycle, and their expression must be downregulated after the corresponding phase of the cell cycle is past. The transcript levels of these tested marker genes were consistently elevated in elo3 leaves (Figure 3e), which suggested that Elongator is required for normal cell-cycle progression
Xu D, Huang W, Li Y, Wang H, Huang H, Cui X - Elongator complex is critical for cell cycle progression and leaf patterning in Arabidopsis
Visit the PubMed ArticleWe then examined transcript levels of several cell cycle-related marker genes by qRT-PCR, using 8-day-old leaves. Cyclin D3;1 (CYCD3;1) is predominantly expressed in the G1-phase and drives the G1/S transition (Dewitte et al., 2003; Menges et al., 2006), H4b is expressed specifically in the S-phase (Soni et al., 1995), while CDT1a, ORC1b, MCM3 and PCNA2 are involved in S-phase DNA replication (Shultz et al., 2007). In addition, CYCA2;1 and CYCB1;1 are involved in S/G2 and G2/M transitions, respectively (Doerner et al., 1996; Shaul et al., 1996). Expression of these markers represents different phases of the cell cycle, and their expression must be downregulated after the corresponding phase of the cell cycle is past. The transcript levels of these tested marker genes were consistently elevated in elo3 leaves (Figure 3e), which suggested that Elongator is required for normal cell-cycle progression
Xu D, Huang W, Li Y, Wang H, Huang H, Cui X - Elongator complex is critical for cell cycle progression and leaf patterning in Arabidopsis
Visit the PubMed ArticleQuantitative real-time polymerase chain reaction (qRT-PCR) analyses of DNA repair and replication checkpoint genes in wild-type, elo3, elp5 and drl1 seedlings ... DNA damage accumulation often results in inductions of DNA repair and DNA replication checkpoint genes; BRAC1, RAD51, PARP2, and WEE1 are such genes in Arabidopsis (Kotogany et al., 2010). We thus examined expression of these genes in the Elongator mutants, and found that transcript levels of all these genes were elevated markedly
Xu D, Huang W, Li Y, Wang H, Huang H, Cui X - Elongator complex is critical for cell cycle progression and leaf patterning in Arabidopsis
Visit the PubMed ArticleQuantitative real-time polymerase chain reaction (qRT-PCR) analyses of DNA repair and replication checkpoint genes in wild-type, elo3, elp5 and drl1 seedlings ... DNA damage accumulation often results in inductions of DNA repair and DNA replication checkpoint genes; BRAC1, RAD51, PARP2, and WEE1 are such genes in Arabidopsis (Kotogany et al., 2010). We thus examined expression of these genes in the Elongator mutants, and found that transcript levels of all these genes were elevated markedly
Xu D, Huang W, Li Y, Wang H, Huang H, Cui X - Elongator complex is critical for cell cycle progression and leaf patterning in Arabidopsis
Visit the PubMed ArticleQuantitative real-time polymerase chain reaction (qRT-PCR) analyses of DNA repair and replication checkpoint genes in wild-type, elo3, elp5 and drl1 seedlings ... DNA damage accumulation often results in inductions of DNA repair and DNA replication checkpoint genes; BRAC1, RAD51, PARP2, and WEE1 are such genes in Arabidopsis (Kotogany et al., 2010). We thus examined expression of these genes in the Elongator mutants, and found that transcript levels of all these genes were elevated markedly
Xu D, Huang W, Li Y, Wang H, Huang H, Cui X - Elongator complex is critical for cell cycle progression and leaf patterning in Arabidopsis
Visit the PubMed ArticleWe then examined transcript levels of several cell cycle-related marker genes by qRT-PCR, using 8-day-old leaves. Cyclin D3;1 (CYCD3;1) is predominantly expressed in the G1-phase and drives the G1/S transition (Dewitte et al., 2003; Menges et al., 2006), H4b is expressed specifically in the S-phase (Soni et al., 1995), while CDT1a, ORC1b, MCM3 and PCNA2 are involved in S-phase DNA replication (Shultz et al., 2007). In addition, CYCA2;1 and CYCB1;1 are involved in S/G2 and G2/M transitions, respectively (Doerner et al., 1996; Shaul et al., 1996). Expression of these markers represents different phases of the cell cycle, and their expression must be downregulated after the corresponding phase of the cell cycle is past. The transcript levels of these tested marker genes were consistently elevated in elo3 leaves (Figure 3e), which suggested that Elongator is required for normal cell-cycle progression
Xu D, Huang W, Li Y, Wang H, Huang H, Cui X - Elongator complex is critical for cell cycle progression and leaf patterning in Arabidopsis
Visit the PubMed ArticleWe next examined expression of leaf polarity marker genes REVOLUTA (REV) and FILAMENTOUS FLOWER (FIL) in the elo2 as2 background. rev-9 is a T-DNA enhancer trap line in which β-glucuronidase (GUS) staining represents the expression of the leaf adaxial marker REV (Emery et al., 2003; Hawker and Bowman, 2004). Compared with that in the rev-9/+ plant (Figure 1q), GUS staining was not detected from needle-like leaves of the elo2 as2 rev-9/+ plant
Xu D, Huang W, Li Y, Wang H, Huang H, Cui X - Elongator complex is critical for cell cycle progression and leaf patterning in Arabidopsis
Visit the PubMed ArticleQuantitative real-time polymerase chain reaction (qRT-PCR) analyses of DNA repair and replication checkpoint genes in wild-type, elo3, elp5 and drl1 seedlings ... DNA damage accumulation often results in inductions of DNA repair and DNA replication checkpoint genes; BRAC1, RAD51, PARP2, and WEE1 are such genes in Arabidopsis (Kotogany et al., 2010). We thus examined expression of these genes in the Elongator mutants, and found that transcript levels of all these genes were elevated markedly
Xu D, Huang W, Li Y, Wang H, Huang H, Cui X - Elongator complex is critical for cell cycle progression and leaf patterning in Arabidopsis
Visit the PubMed ArticleQuantitative real-time polymerase chain reaction (qRT-PCR) analyses of DNA repair and replication checkpoint genes in wild-type, elo3, elp5 and drl1 seedlings ... DNA damage accumulation often results in inductions of DNA repair and DNA replication checkpoint genes; BRAC1, RAD51, PARP2, and WEE1 are such genes in Arabidopsis (Kotogany et al., 2010). We thus examined expression of these genes in the Elongator mutants, and found that transcript levels of all these genes were elevated markedly
Xu D, Huang W, Li Y, Wang H, Huang H, Cui X - Elongator complex is critical for cell cycle progression and leaf patterning in Arabidopsis
Visit the PubMed ArticleTo investigate the cellular basis of the Elongator mutants, we analyzed expression patterns of a cell division marker histone H4 by in situ hybridization ... Compared with those in the wild type, H4 signals in the proximal and abaxial domains of elo2 leaf primordia weakened much earlier ... Figure 2b, blue lines; Figure 2d). In some extreme cases, a property of elo2 single mutant plants produced the needle-like leaves, and these structures exhibited even less H4 signals
Xu D, Huang W, Li Y, Wang H, Huang H, Cui X - Elongator complex is critical for cell cycle progression and leaf patterning in Arabidopsis
Visit the PubMed ArticleQuantitative real-time polymerase chain reaction (qRT-PCR) analyses of DNA repair and replication checkpoint genes in wild-type, elo3, elp5 and drl1 seedlings ... DNA damage accumulation often results in inductions of DNA repair and DNA replication checkpoint genes; BRAC1, RAD51, PARP2, and WEE1 are such genes in Arabidopsis (Kotogany et al., 2010). We thus examined expression of these genes in the Elongator mutants, and found that transcript levels of all these genes were elevated markedly
Xu D, Huang W, Li Y, Wang H, Huang H, Cui X - Elongator complex is critical for cell cycle progression and leaf patterning in Arabidopsis
Visit the PubMed ArticleWe then examined transcript levels of several cell cycle-related marker genes by qRT-PCR, using 8-day-old leaves. Cyclin D3;1 (CYCD3;1) is predominantly expressed in the G1-phase and drives the G1/S transition (Dewitte et al., 2003; Menges et al., 2006), H4b is expressed specifically in the S-phase (Soni et al., 1995), while CDT1a, ORC1b, MCM3 and PCNA2 are involved in S-phase DNA replication (Shultz et al., 2007). In addition, CYCA2;1 and CYCB1;1 are involved in S/G2 and G2/M transitions, respectively (Doerner et al., 1996; Shaul et al., 1996). Expression of these markers represents different phases of the cell cycle, and their expression must be downregulated after the corresponding phase of the cell cycle is past. The transcript levels of these tested marker genes were consistently elevated in elo3 leaves (Figure 3e), which suggested that Elongator is required for normal cell-cycle progression
Xu D, Huang W, Li Y, Wang H, Huang H, Cui X - Elongator complex is critical for cell cycle progression and leaf patterning in Arabidopsis
Visit the PubMed ArticleQuantitative real-time polymerase chain reaction (qRT-PCR) analyses of DNA repair and replication checkpoint genes in wild-type, elo3, elp5 and drl1 seedlings ... DNA damage accumulation often results in inductions of DNA repair and DNA replication checkpoint genes; BRAC1, RAD51, PARP2, and WEE1 are such genes in Arabidopsis (Kotogany et al., 2010). We thus examined expression of these genes in the Elongator mutants, and found that transcript levels of all these genes were elevated markedly
Xu D, Huang W, Li Y, Wang H, Huang H, Cui X - Elongator complex is critical for cell cycle progression and leaf patterning in Arabidopsis
Visit the PubMed ArticleWe then examined transcript levels of several cell cycle-related marker genes by qRT-PCR, using 8-day-old leaves. Cyclin D3;1 (CYCD3;1) is predominantly expressed in the G1-phase and drives the G1/S transition (Dewitte et al., 2003; Menges et al., 2006), H4b is expressed specifically in the S-phase (Soni et al., 1995), while CDT1a, ORC1b, MCM3 and PCNA2 are involved in S-phase DNA replication (Shultz et al., 2007). In addition, CYCA2;1 and CYCB1;1 are involved in S/G2 and G2/M transitions, respectively (Doerner et al., 1996; Shaul et al., 1996). Expression of these markers represents different phases of the cell cycle, and their expression must be downregulated after the corresponding phase of the cell cycle is past. The transcript levels of these tested marker genes were consistently elevated in elo3 leaves (Figure 3e), which suggested that Elongator is required for normal cell-cycle progression
Xu D, Huang W, Li Y, Wang H, Huang H, Cui X - Elongator complex is critical for cell cycle progression and leaf patterning in Arabidopsis
Visit the PubMed ArticleWe then examined transcript levels of several cell cycle-related marker genes by qRT-PCR, using 8-day-old leaves. Cyclin D3;1 (CYCD3;1) is predominantly expressed in the G1-phase and drives the G1/S transition (Dewitte et al., 2003; Menges et al., 2006), H4b is expressed specifically in the S-phase (Soni et al., 1995), while CDT1a, ORC1b, MCM3 and PCNA2 are involved in S-phase DNA replication (Shultz et al., 2007). In addition, CYCA2;1 and CYCB1;1 are involved in S/G2 and G2/M transitions, respectively (Doerner et al., 1996; Shaul et al., 1996). Expression of these markers represents different phases of the cell cycle, and their expression must be downregulated after the corresponding phase of the cell cycle is past. The transcript levels of these tested marker genes were consistently elevated in elo3 leaves (Figure 3e), which suggested that Elongator is required for normal cell-cycle progression
Xu D, Huang W, Li Y, Wang H, Huang H, Cui X - Elongator complex is critical for cell cycle progression and leaf patterning in Arabidopsis
Visit the PubMed ArticleIn some extreme cases, a property of elo2 single mutant plants produced the needle-like leaves, and these structures exhibited even less H4 signals (Figure 2e, arrows). However, FIL hybridization signals were detected throughout primordia of needle-like leaves
Xu D, Huang W, Li Y, Wang H, Huang H, Cui X - Elongator complex is critical for cell cycle progression and leaf patterning in Arabidopsis
Visit the PubMed ArticleTo investigate the cellular basis of the Elongator mutants, we analyzed expression patterns of a cell division marker histone H4 by in situ hybridization ... Compared with those in the wild type, H4 signals in the proximal and abaxial domains of elo2 leaf primordia weakened much earlier ... Figure 2b, blue lines; Figure 2d). In some extreme cases, a property of elo2 single mutant plants produced the needle-like leaves, and these structures exhibited even less H4 signals
Xu D, Huang W, Li Y, Wang H, Huang H, Cui X - Elongator complex is critical for cell cycle progression and leaf patterning in Arabidopsis
Visit the PubMed ArticleQuantitative real-time polymerase chain reaction (qRT-PCR) analyses of DNA repair and replication checkpoint genes in wild-type, elo3, elp5 and drl1 seedlings ... DNA damage accumulation often results in inductions of DNA repair and DNA replication checkpoint genes; BRAC1, RAD51, PARP2, and WEE1 are such genes in Arabidopsis (Kotogany et al., 2010). We thus examined expression of these genes in the Elongator mutants, and found that transcript levels of all these genes were elevated markedly
Xu D, Huang W, Li Y, Wang H, Huang H, Cui X - Elongator complex is critical for cell cycle progression and leaf patterning in Arabidopsis
Visit the PubMed ArticleWe then examined transcript levels of several cell cycle-related marker genes by qRT-PCR, using 8-day-old leaves. Cyclin D3;1 (CYCD3;1) is predominantly expressed in the G1-phase and drives the G1/S transition (Dewitte et al., 2003; Menges et al., 2006), H4b is expressed specifically in the S-phase (Soni et al., 1995), while CDT1a, ORC1b, MCM3 and PCNA2 are involved in S-phase DNA replication (Shultz et al., 2007). In addition, CYCA2;1 and CYCB1;1 are involved in S/G2 and G2/M transitions, respectively (Doerner et al., 1996; Shaul et al., 1996). Expression of these markers represents different phases of the cell cycle, and their expression must be downregulated after the corresponding phase of the cell cycle is past. The transcript levels of these tested marker genes were consistently elevated in elo3 leaves (Figure 3e), which suggested that Elongator is required for normal cell-cycle progression
Xu D, Huang W, Li Y, Wang H, Huang H, Cui X - Elongator complex is critical for cell cycle progression and leaf patterning in Arabidopsis
Visit the PubMed ArticleQuantitative real-time polymerase chain reaction (qRT-PCR) analyses of DNA repair and replication checkpoint genes in wild-type, elo3, elp5 and drl1 seedlings ... DNA damage accumulation often results in inductions of DNA repair and DNA replication checkpoint genes; BRAC1, RAD51, PARP2, and WEE1 are such genes in Arabidopsis (Kotogany et al., 2010). We thus examined expression of these genes in the Elongator mutants, and found that transcript levels of all these genes were elevated markedly
Xu D, Huang W, Li Y, Wang H, Huang H, Cui X - Elongator complex is critical for cell cycle progression and leaf patterning in Arabidopsis
Visit the PubMed Articlein the atgstu17-1 and atgstu17-2 lines ... microarray data ... the DHAR2 gene ... for dehydroascorbate reductase; AT1G75270), involved in the production of ascorbate, also increased 1.97-fold
Chen JH, Jiang HW, Hsieh EJ, Chen HY, Chien CT, Hsieh HL, Lin TP - Drought and salt stress tolerance of an Arabidopsis glutathione S-transferase U17 knockout mutant are attributed to the combined effect of glutathione and abscisic acid
Visit the PubMed Articlewe performed real-time PCR analysis ... AREB1 ... up-regulated in the atgstu17-1 and atgstu17-2 lines
Chen JH, Jiang HW, Hsieh EJ, Chen HY, Chien CT, Hsieh HL, Lin TP - Drought and salt stress tolerance of an Arabidopsis glutathione S-transferase U17 knockout mutant are attributed to the combined effect of glutathione and abscisic acid
Visit the PubMed Articlein the atgstu17-1 and atgstu17-2 lines ... microarray data ... the GLUTATHIONE SYNTHETASE2 (GSH2) gene (AT5G27380), catalyzing the ligation of Gly to the Cys residue of γ-glutamyl-Cys to form GSH, was up 1.68-fold
Chen JH, Jiang HW, Hsieh EJ, Chen HY, Chien CT, Hsieh HL, Lin TP - Drought and salt stress tolerance of an Arabidopsis glutathione S-transferase U17 knockout mutant are attributed to the combined effect of glutathione and abscisic acid
Visit the PubMed Articlewe performed real-time PCR analysis ... AnnAt1 ... up-regulated in the atgstu17-1 and atgstu17-2 lines
Chen JH, Jiang HW, Hsieh EJ, Chen HY, Chien CT, Hsieh HL, Lin TP - Drought and salt stress tolerance of an Arabidopsis glutathione S-transferase U17 knockout mutant are attributed to the combined effect of glutathione and abscisic acid
Visit the PubMed Articlewe performed real-time PCR analysis ... XERICO ... up-regulated in the atgstu17-1 and atgstu17-2 lines
Chen JH, Jiang HW, Hsieh EJ, Chen HY, Chien CT, Hsieh HL, Lin TP - Drought and salt stress tolerance of an Arabidopsis glutathione S-transferase U17 knockout mutant are attributed to the combined effect of glutathione and abscisic acid
Visit the PubMed Articlewe performed real-time PCR analysis ... COR15b ... up-regulated in the atgstu17-1 and atgstu17-2 lines
Chen JH, Jiang HW, Hsieh EJ, Chen HY, Chien CT, Hsieh HL, Lin TP - Drought and salt stress tolerance of an Arabidopsis glutathione S-transferase U17 knockout mutant are attributed to the combined effect of glutathione and abscisic acid
Visit the PubMed Articlewe performed real-time PCR analysis ... Bax inhibitor-1 ... up-regulated in the atgstu17-1 and atgstu17-2 lines
Chen JH, Jiang HW, Hsieh EJ, Chen HY, Chien CT, Hsieh HL, Lin TP - Drought and salt stress tolerance of an Arabidopsis glutathione S-transferase U17 knockout mutant are attributed to the combined effect of glutathione and abscisic acid
Visit the PubMed Articlewe performed real-time PCR analysis ... AtMYB88 ... up-regulated in the atgstu17-1 and atgstu17-2 lines
Chen JH, Jiang HW, Hsieh EJ, Chen HY, Chien CT, Hsieh HL, Lin TP - Drought and salt stress tolerance of an Arabidopsis glutathione S-transferase U17 knockout mutant are attributed to the combined effect of glutathione and abscisic acid
Visit the PubMed ArticleCDS2 ... mRNA levels did not show any substantial alteration in the bio4-1 mutant
Li J, Brader G, Helenius E, Kariola T, Palva ET - Biotin deficiency causes spontaneous cell death and activation of defense signaling
Visit the PubMed Articlebio4-1-dependent up-regulation of PR1 is further enhanced in bio4-1/coi1-1 double mutants, which are defective in JA signaling, consistent with possible negative feedback regulation in JA- and SA-related signaling
Li J, Brader G, Helenius E, Kariola T, Palva ET - Biotin deficiency causes spontaneous cell death and activation of defense signaling
Visit the PubMed Articlethe levels of protein of the size of mitochondrial-localized AtMCC-A were barely affected in bio4-1
Li J, Brader G, Helenius E, Kariola T, Palva ET - Biotin deficiency causes spontaneous cell death and activation of defense signaling
Visit the PubMed Articleexpression of GST1 was barely altered in the ... bio4-1/coi1-1 double mutants ... indicating that the up-regulation of GST1 expression in bio4-1 is independent of ... JA signaling
Li J, Brader G, Helenius E, Kariola T, Palva ET - Biotin deficiency causes spontaneous cell death and activation of defense signaling
Visit the PubMed ArticleWe also considered the possibility that reduced levels of hitherto uncharacterized biotin-containing proteins may contribute to the observed phenotype in the bio4-1 mutant. To assess this, we isolated nuclei and concentrated nuclear proteins (Turck et al., 2004). Three bands of sizes 80 kDa (data not shown) and 37 and 35 kDa (Figure 5b) were present in the wild-type, but only the 80 kDa band was visible in the bio4-1 mutant. Most likely, the bands with sizes of 80 and 35 kDa represent contamination with the non-nuclear biotin-containing proteins AtMCC-A and AtBCCP1. Interestingly, a protein of size 37 kDa was present in the wild-type but absent in bio4-1 (Figure 5b). Our data therefore indicate the existence of an additional, so far uncharacterized, biotinylated nuclear-localized protein
Li J, Brader G, Helenius E, Kariola T, Palva ET - Biotin deficiency causes spontaneous cell death and activation of defense signaling
Visit the PubMed Articlebio4-1 mutant ... GST1 transcripts encoding a glutathionine S-transferase implicated in stress responses such as pathogen attack and oxidative stress accumulated in the mutant
Li J, Brader G, Helenius E, Kariola T, Palva ET - Biotin deficiency causes spontaneous cell death and activation of defense signaling
Visit the PubMed ArticleCDS1 ... mRNA levels did not show any substantial alteration in the bio4-1 mutant
Li J, Brader G, Helenius E, Kariola T, Palva ET - Biotin deficiency causes spontaneous cell death and activation of defense signaling
Visit the PubMed ArticleMSD ... mRNA levels did not show any substantial alteration in the bio4-1 mutant
Li J, Brader G, Helenius E, Kariola T, Palva ET - Biotin deficiency causes spontaneous cell death and activation of defense signaling
Visit the PubMed ArticleSeveral biotin-containing proteins have been characterized in Arabidopsis plants, including the chloroplast-localized biotin carboxyl carrier proteins AtBCCP1 (approximately 35 kDa) (Choi et al., 1995) and AtBCCP2 (approximately 25 kDa) (Thelen et al., 2001), as well as a biotinyl subunit (also referred to referred as to AtMCC-A; Che et al., 2003) of mitochondrial-localized methylcrotonoyl CoA carboxylase (MCCase) of approximately 80 kDa. To investigate whether the T-DNA insertion in the bio4-1 locus leads to reduced levels of biotin-containing proteins, we detected biotin-containing proteins with streptavidin peroxidase based on the streptavidin–biotin interaction. Western blot analysis revealed that a polypeptide with an approximate size of 80 kDa was predominant in mutant and wild-type leaf extracts, and an additional 35 kDa polypeptide was only weakly present in the mutant. A 25 kDa polypeptide was only detectable in wild-type plants (Figure 5a). These results are consistent with previous observations in wild-type leaves of Arabidopsis (Che et al., 2003; Thelen et al., 2001). Interestingly, the amounts of proteins of the same size as the chloroplast-localized biotin-containing proteins ... AtBCCP2 ... strongly reduced in the bio4-1 mutant
Li J, Brader G, Helenius E, Kariola T, Palva ET - Biotin deficiency causes spontaneous cell death and activation of defense signaling
Visit the PubMed ArticleSeveral biotin-containing proteins have been characterized in Arabidopsis plants, including the chloroplast-localized biotin carboxyl carrier proteins AtBCCP1 (approximately 35 kDa) (Choi et al., 1995) and AtBCCP2 (approximately 25 kDa) (Thelen et al., 2001), as well as a biotinyl subunit (also referred to referred as to AtMCC-A; Che et al., 2003) of mitochondrial-localized methylcrotonoyl CoA carboxylase (MCCase) of approximately 80 kDa. To investigate whether the T-DNA insertion in the bio4-1 locus leads to reduced levels of biotin-containing proteins, we detected biotin-containing proteins with streptavidin peroxidase based on the streptavidin–biotin interaction. Western blot analysis revealed that a polypeptide with an approximate size of 80 kDa was predominant in mutant and wild-type leaf extracts, and an additional 35 kDa polypeptide was only weakly present in the mutant. A 25 kDa polypeptide was only detectable in wild-type plants (Figure 5a). These results are consistent with previous observations in wild-type leaves of Arabidopsis (Che et al., 2003; Thelen et al., 2001). Interestingly, the amounts of proteins of the same size as the chloroplast-localized biotin-containing proteins AtBCCP1 ... strongly reduced in the bio4-1 mutant
Li J, Brader G, Helenius E, Kariola T, Palva ET - Biotin deficiency causes spontaneous cell death and activation of defense signaling
Visit the PubMed Articlewe characterized expression of these genes in the double mutant bio4-1/npr1-1 by RNA gel-blot analysis ... up-regulation of ... PR2 ... was still evident
Li J, Brader G, Helenius E, Kariola T, Palva ET - Biotin deficiency causes spontaneous cell death and activation of defense signaling
Visit the PubMed Articlewe characterized expression of these genes in the double mutant bio4-1/npr1-1 by RNA gel-blot analysis ... up-regulation of PR1 ... was still evident
Li J, Brader G, Helenius E, Kariola T, Palva ET - Biotin deficiency causes spontaneous cell death and activation of defense signaling
Visit the PubMed Articleexpression of GST1 was barely altered in the bio4-1/npr1-1 ... indicating that the up-regulation of GST1 expression in bio4-1 is independent of functional NPR1
Li J, Brader G, Helenius E, Kariola T, Palva ET - Biotin deficiency causes spontaneous cell death and activation of defense signaling
Visit the PubMed Articleexpression of ... PR5 is clearly enhanced in the leaves of the bio4-1 mutant, confirming the results obtained in the microarray
Li J, Brader G, Helenius E, Kariola T, Palva ET - Biotin deficiency causes spontaneous cell death and activation of defense signaling
Visit the PubMed Articlewe characterized expression of these genes in the double mutant bio4-1/npr1-1 by RNA gel-blot analysis ... up-regulation of ... PR5 was still evident
Li J, Brader G, Helenius E, Kariola T, Palva ET - Biotin deficiency causes spontaneous cell death and activation of defense signaling
Visit the PubMed Articleexpression of ... PR2 ... is clearly enhanced in the leaves of the bio4-1 mutant, confirming the results obtained in the microarray
Li J, Brader G, Helenius E, Kariola T, Palva ET - Biotin deficiency causes spontaneous cell death and activation of defense signaling
Visit the PubMed ArticleFSD1 ... mRNA levels did not show any substantial alteration in the bio4-1 mutant
Li J, Brader G, Helenius E, Kariola T, Palva ET - Biotin deficiency causes spontaneous cell death and activation of defense signaling
Visit the PubMed Articleexpression of PR1 ... is clearly enhanced in the leaves of the bio4-1 mutant, confirming the results obtained in the microarray
Li J, Brader G, Helenius E, Kariola T, Palva ET - Biotin deficiency causes spontaneous cell death and activation of defense signaling
Visit the PubMed Articlethe constitutive expression of PR1 in bio4-1 was significantly reduced but not completely abolished in bio4-1/NahG plants that are unable to accumulate SA
Li J, Brader G, Helenius E, Kariola T, Palva ET - Biotin deficiency causes spontaneous cell death and activation of defense signaling
Visit the PubMed ArticleSLG1::GUS ... activity was detected in all areas of leaf tissues, including both vascular tissues and inter-vein areas. Expression of GUS was higher in old leaves than in young leaves
Yuan H, Liu D - Functional disruption of the pentatricopeptide protein SLG1 affects mitochondrial RNA editing, plant development, and responses to abiotic stresses in Arabidopsis
Visit the PubMed ArticleSLG1::GUS was expressed throughout the seedling after germination (Figure 5b) and persisted during seedling development
Yuan H, Liu D - Functional disruption of the pentatricopeptide protein SLG1 affects mitochondrial RNA editing, plant development, and responses to abiotic stresses in Arabidopsis
Visit the PubMed ArticleQuantitative real-time PCR analysis showed that the levels of SLG1 mRNA were similar among roots, leaves, stems, and siliques but relatively high in flowers
Yuan H, Liu D - Functional disruption of the pentatricopeptide protein SLG1 affects mitochondrial RNA editing, plant development, and responses to abiotic stresses in Arabidopsis
Visit the PubMed ArticleThe TargetP 1.1 program (http://www.cbs.dtu.dk/services/TargetP/) predicted that SLG1 contains a 14-amino-acid signal peptide at its N-terminus for mitochondrial targeting. To experimentally confirm its subcellular localization, we fused the whole SLG1 sequence with a GFP gene. This fusion gene was transiently co-expressed with an mCherry-tagged mitochondrion-specific marker gene (mt-mCherry) under the control of the 35S promoter in Arabidopsis protoplasts (Nelson et al., 2007). The results showed that the green fluorescent signal of SLG1-GFP was co-localized with the red fluorescent signal from the mt-mCherry marker gene (Figure 5c), which provided direct evidence that SLG1 is a mitochondrion-localized protein
Yuan H, Liu D - Functional disruption of the pentatricopeptide protein SLG1 affects mitochondrial RNA editing, plant development, and responses to abiotic stresses in Arabidopsis
Visit the PubMed Articlewe examined the transcript levels of six ND genes and three AOX genes by quantitative real-time PCR. The expression levels of six of nine genes examined in slg1 increased significantly (2- to 20-fold) relative to the WT
Yuan H, Liu D - Functional disruption of the pentatricopeptide protein SLG1 affects mitochondrial RNA editing, plant development, and responses to abiotic stresses in Arabidopsis
Visit the PubMed Articlewe found increased ... CDKA;1 protein levels in the pE2FA::gE2FA–GFP
Magyar Z, Horváth B, Khan S, Mohammed B, Henriques R, De Veylder L, Bakó L, Scheres B, Bögre L - Arabidopsis E2FA stimulates proliferation and endocycle separately through RBR-bound and RBR-free complexes
Visit the PubMed Articlein the CYCD3;1 overexpression line ... elevated levels of ... CDKB1 ... proteins
Magyar Z, Horváth B, Khan S, Mohammed B, Henriques R, De Veylder L, Bakó L, Scheres B, Bögre L - Arabidopsis E2FA stimulates proliferation and endocycle separately through RBR-bound and RBR-free complexes
Visit the PubMed ArticleRBR1 ... protein amount increased proportionally to the level of E2FA–GFP
Magyar Z, Horváth B, Khan S, Mohammed B, Henriques R, De Veylder L, Bakó L, Scheres B, Bögre L - Arabidopsis E2FA stimulates proliferation and endocycle separately through RBR-bound and RBR-free complexes
Visit the PubMed ArticleRBR1–GFP were abundant in proliferating cells
Magyar Z, Horváth B, Khan S, Mohammed B, Henriques R, De Veylder L, Bakó L, Scheres B, Bögre L - Arabidopsis E2FA stimulates proliferation and endocycle separately through RBR-bound and RBR-free complexes
Visit the PubMed ArticleIn young leaves ... E2FA–GFP ... were abundant in proliferating cells
Magyar Z, Horváth B, Khan S, Mohammed B, Henriques R, De Veylder L, Bakó L, Scheres B, Bögre L - Arabidopsis E2FA stimulates proliferation and endocycle separately through RBR-bound and RBR-free complexes
Visit the PubMed Articlemore E2FA protein was present in the young proliferating leaves (9 DAG ... and it diminished as leaves develop ... 11–16 DAG
Magyar Z, Horváth B, Khan S, Mohammed B, Henriques R, De Veylder L, Bakó L, Scheres B, Bögre L - Arabidopsis E2FA stimulates proliferation and endocycle separately through RBR-bound and RBR-free complexes
Visit the PubMed ArticleE2FB ... downregulated ... E2FA silencing line
Magyar Z, Horváth B, Khan S, Mohammed B, Henriques R, De Veylder L, Bakó L, Scheres B, Bögre L - Arabidopsis E2FA stimulates proliferation and endocycle separately through RBR-bound and RBR-free complexes
Visit the PubMed ArticleAlthough we did not detect a significant increase in ... RBR1 transcripts, their protein amount increased proportionally to the level of E2FA–GFP
Magyar Z, Horváth B, Khan S, Mohammed B, Henriques R, De Veylder L, Bakó L, Scheres B, Bögre L - Arabidopsis E2FA stimulates proliferation and endocycle separately through RBR-bound and RBR-free complexes
Visit the PubMed Articlewe found increased ... CDKB1;1 ... protein levels in the pE2FA::gE2FA–GFP
Magyar Z, Horváth B, Khan S, Mohammed B, Henriques R, De Veylder L, Bakó L, Scheres B, Bögre L - Arabidopsis E2FA stimulates proliferation and endocycle separately through RBR-bound and RBR-free complexes
Visit the PubMed Articleyoung leaves ... E2FA–GFP ... detectable in differentiated pavement cells
Magyar Z, Horváth B, Khan S, Mohammed B, Henriques R, De Veylder L, Bakó L, Scheres B, Bögre L - Arabidopsis E2FA stimulates proliferation and endocycle separately through RBR-bound and RBR-free complexes
Visit the PubMed Articlein the CYCD3;1 overexpression ... elevated levels of E2FB ... proteins
Magyar Z, Horváth B, Khan S, Mohammed B, Henriques R, De Veylder L, Bakó L, Scheres B, Bögre L - Arabidopsis E2FA stimulates proliferation and endocycle separately through RBR-bound and RBR-free complexes
Visit the PubMed ArticleNULL
Magyar Z, Horváth B, Khan S, Mohammed B, Henriques R, De Veylder L, Bakó L, Scheres B, Bögre L - Arabidopsis E2FA stimulates proliferation and endocycle separately through RBR-bound and RBR-free complexes
Visit the PubMed ArticleE2FB level is slightly upregulated in the E2FA/DPA overexpression line
Magyar Z, Horváth B, Khan S, Mohammed B, Henriques R, De Veylder L, Bakó L, Scheres B, Bögre L - Arabidopsis E2FA stimulates proliferation and endocycle separately through RBR-bound and RBR-free complexes
Visit the PubMed ArticleORC2 ... downregulated ... E2FA silencing line
Magyar Z, Horváth B, Khan S, Mohammed B, Henriques R, De Veylder L, Bakó L, Scheres B, Bögre L - Arabidopsis E2FA stimulates proliferation and endocycle separately through RBR-bound and RBR-free complexes
Visit the PubMed ArticleIn young leaves ... RBR1–GFP ... detectable in differentiated pavement cells
Magyar Z, Horváth B, Khan S, Mohammed B, Henriques R, De Veylder L, Bakó L, Scheres B, Bögre L - Arabidopsis E2FA stimulates proliferation and endocycle separately through RBR-bound and RBR-free complexes
Visit the PubMed Articlereduced CDKB1;1 protein level in the E2FA-RNAi plants
Magyar Z, Horváth B, Khan S, Mohammed B, Henriques R, De Veylder L, Bakó L, Scheres B, Bögre L - Arabidopsis E2FA stimulates proliferation and endocycle separately through RBR-bound and RBR-free complexes
Visit the PubMed ArticleRBR1 ... downregulated ... E2FA silencing line
Magyar Z, Horváth B, Khan S, Mohammed B, Henriques R, De Veylder L, Bakó L, Scheres B, Bögre L - Arabidopsis E2FA stimulates proliferation and endocycle separately through RBR-bound and RBR-free complexes
Visit the PubMed Articlein the CYCD3;1 overexpression line ... elevated levels of ... DPA ... proteins
Magyar Z, Horváth B, Khan S, Mohammed B, Henriques R, De Veylder L, Bakó L, Scheres B, Bögre L - Arabidopsis E2FA stimulates proliferation and endocycle separately through RBR-bound and RBR-free complexes
Visit the PubMed Articlethe E2FA/DPA overexpression plants showed a strongly upregulated RBR1 amount
Magyar Z, Horváth B, Khan S, Mohammed B, Henriques R, De Veylder L, Bakó L, Scheres B, Bögre L - Arabidopsis E2FA stimulates proliferation and endocycle separately through RBR-bound and RBR-free complexes
Visit the PubMed ArticleMCM3 ... downregulated ... E2FA silencing line
Magyar Z, Horváth B, Khan S, Mohammed B, Henriques R, De Veylder L, Bakó L, Scheres B, Bögre L - Arabidopsis E2FA stimulates proliferation and endocycle separately through RBR-bound and RBR-free complexes
Visit the PubMed ArticleNULL
Magyar Z, Horváth B, Khan S, Mohammed B, Henriques R, De Veylder L, Bakó L, Scheres B, Bögre L - Arabidopsis E2FA stimulates proliferation and endocycle separately through RBR-bound and RBR-free complexes
Visit the PubMed ArticleDPA ... protein amount increased proportionally to the level of E2FA–GFP
Magyar Z, Horváth B, Khan S, Mohammed B, Henriques R, De Veylder L, Bakó L, Scheres B, Bögre L - Arabidopsis E2FA stimulates proliferation and endocycle separately through RBR-bound and RBR-free complexes
Visit the PubMed ArticleAlthough we did not detect a significant increase in DPA ... transcripts, their protein amount increased proportionally to the level of E2FA–GFP
Magyar Z, Horváth B, Khan S, Mohammed B, Henriques R, De Veylder L, Bakó L, Scheres B, Bögre L - Arabidopsis E2FA stimulates proliferation and endocycle separately through RBR-bound and RBR-free complexes
Visit the PubMed ArticleExpression of the NTL4 gene was induced more than twofold by air-drying
Lee S, Seo PJ, Lee HJ, Park CM - Accelerated cell death 2 suppresses mitochondrial oxidative bursts and modulates cell death in Arabidopsis
Visit the PubMed ArticleExpression of the NTL4 gene was induced more than fourfold 8 days following the start of drought
Lee S, Seo PJ, Lee HJ, Park CM - Accelerated cell death 2 suppresses mitochondrial oxidative bursts and modulates cell death in Arabidopsis
Visit the PubMed Articledrought had no effect on on expression of the NTL4 ... in the ABA-deficient aba3-1 mutant ... suggesting that the NTL4-mediated drought stress signals depend on ABA
Lee S, Seo PJ, Lee HJ, Park CM - Accelerated cell death 2 suppresses mitochondrial oxidative bursts and modulates cell death in Arabidopsis
Visit the PubMed ArticleThe transcript levels of other Atrboh genes, such as ... AtrbohF ... were slightly altered in 35S:4ΔC transgenic plants and the ntl4-1 mutant under both normal and drought conditions (Figure S6). However, the degrees of alterations were not significant, and the alteration patterns did not match the predicted role of NTL4 in drought stress responses, suggesting that these Atrboh genes are not directly related to NTL4
Lee S, Seo PJ, Lee HJ, Park CM - Accelerated cell death 2 suppresses mitochondrial oxidative bursts and modulates cell death in Arabidopsis
Visit the PubMed ArticleAtrbohC ... The inductive effects of drought on the Atrboh gene expression were diminished in the ntl4-1 mutant
Lee S, Seo PJ, Lee HJ, Park CM - Accelerated cell death 2 suppresses mitochondrial oxidative bursts and modulates cell death in Arabidopsis
Visit the PubMed Articlewe first examined the expression of genes encoding antioxidant metabolic enzymes, such as those encoded by VITAMIN C DEFECTIVE 1 (VTC1) ... They were not much affected in 35S:4ΔC transgenic plants and the ntl4-1 mutant
Lee S, Seo PJ, Lee HJ, Park CM - Accelerated cell death 2 suppresses mitochondrial oxidative bursts and modulates cell death in Arabidopsis
Visit the PubMed Articleheat treatments
Lee S, Seo PJ, Lee HJ, Park CM - Accelerated cell death 2 suppresses mitochondrial oxidative bursts and modulates cell death in Arabidopsis
Visit the PubMed ArticleExpression of the NTL4 gene was induced rapidly in older plants that exhibit symptoms of senescence
Lee S, Seo PJ, Lee HJ, Park CM - Accelerated cell death 2 suppresses mitochondrial oxidative bursts and modulates cell death in Arabidopsis
Visit the PubMed ArticleTo further examine the effects of drought on NTL4 gene expression, the GUS coding sequence was fused in-frame to the NTL4 gene promoter comprising approximately 1 kb upstream sequence, and the promoter–GUS fusion was transformed into Col-0 plants. GUS activity was detected mainly in the roots under normal conditions (Figure 2e). When the transgenic plants were exposed to air drying, GUS activity was significantly elevated in the aerial plant parts, primarily in the distal leaf area where the leaf senescence process initiates under adverse growth conditions
Lee S, Seo PJ, Lee HJ, Park CM - Accelerated cell death 2 suppresses mitochondrial oxidative bursts and modulates cell death in Arabidopsis
Visit the PubMed ArticleAtrbohA ... The inductive effects of drought on the Atrboh gene expression were diminished in the ntl4-1 mutant.
Lee S, Seo PJ, Lee HJ, Park CM - Accelerated cell death 2 suppresses mitochondrial oxidative bursts and modulates cell death in Arabidopsis
Visit the PubMed ArticleWe also examined the patterns of NTL4 processing in drought-treated plants. The intensity of the processed NTL4 form was markedly increased (Figure 9b), similar to what is observed in ABA-treated plants
Lee S, Seo PJ, Lee HJ, Park CM - Accelerated cell death 2 suppresses mitochondrial oxidative bursts and modulates cell death in Arabidopsis
Visit the PubMed Articlewe first examined the expression of genes encoding antioxidant metabolic enzymes, such as those encoded by ... CADMIUM SENSITIVE 2 (CAD2) ... They were not much affected in 35S:4ΔC transgenic plants and the ntl4-1 mutant
Lee S, Seo PJ, Lee HJ, Park CM - Accelerated cell death 2 suppresses mitochondrial oxidative bursts and modulates cell death in Arabidopsis
Visit the PubMed ArticleTen-day-old 35S:MYC-NTL4 transgenic plants were treated with ABA. Immunological assays revealed that NTL4 processing does not occur to a detectable level in transgenic plants treated with ABA for up to 10 h (Figure S8). In contrast, a protein band with an estimated molecular mass close to the calculated molecular mass of the MYC–4ΔC fusion (10.5 + 46.2 kDa) was detected in plants that were ABA-treated for 24 h (Figure 9a), showing that NTL4 processing is a long-term process. When the plants were treated with the potent proteasome inhibitor MG132, both the full-size and processed NTL4 forms increased, suggesting that the ubiquitin-mediated degradation pathway is responsible for the observed rapid turnover of NTL4 proteins
Lee S, Seo PJ, Lee HJ, Park CM - Accelerated cell death 2 suppresses mitochondrial oxidative bursts and modulates cell death in Arabidopsis
Visit the PubMed ArticleQuantitative real-time RT-PCR assays showed that the transcript levels of ... AtrbohE ... more than ninefold higher in 35S:4ΔC transgenic plants than in Col-0 plants under normal conditions (Figure 6d). Under drought conditions, expression of the Atrboh genes was further induced at least twofold in both Col-0 plants and 35S:4ΔC transgenic plants, showing that NTL4-mediated drought stress signals up-regulate a subset of the Atrboh genes
Lee S, Seo PJ, Lee HJ, Park CM - Accelerated cell death 2 suppresses mitochondrial oxidative bursts and modulates cell death in Arabidopsis
Visit the PubMed ArticleThe transcript levels of other Atrboh genes, such as ... AtrbohG, were slightly altered in 35S:4ΔC transgenic plants and the ntl4-1 mutant under both normal and drought conditions (Figure S6). However, the degrees of alterations were not significant, and the alteration patterns did not match the predicted role of NTL4 in drought stress responses, suggesting that these Atrboh genes are not directly related to NTL4
Lee S, Seo PJ, Lee HJ, Park CM - Accelerated cell death 2 suppresses mitochondrial oxidative bursts and modulates cell death in Arabidopsis
Visit the PubMed ArticleWe found that the SAG12 gene is induced at ... later growth stages in the ntl4-1 mutant
Lee S, Seo PJ, Lee HJ, Park CM - Accelerated cell death 2 suppresses mitochondrial oxidative bursts and modulates cell death in Arabidopsis
Visit the PubMed Articledrought had no effect on on expression of the ... AtrbohC ... in the ABA-deficient aba3-1 mutant
Lee S, Seo PJ, Lee HJ, Park CM - Accelerated cell death 2 suppresses mitochondrial oxidative bursts and modulates cell death in Arabidopsis
Visit the PubMed ArticleThe transcript levels of other Atrboh genes, such as AtrbohB ... were slightly altered in 35S:4ΔC transgenic plants and the ntl4-1 mutant under both normal and drought conditions (Figure S6). However, the degrees of alterations were not significant, and the alteration patterns did not match the predicted role of NTL4 in drought stress responses, suggesting that these Atrboh genes are not directly related to NTL4
Lee S, Seo PJ, Lee HJ, Park CM - Accelerated cell death 2 suppresses mitochondrial oxidative bursts and modulates cell death in Arabidopsis
Visit the PubMed ArticleQuantitative real-time RT-PCR assays showed that the transcript levels of ... AtrbohC ... more than ninefold higher in 35S:4ΔC transgenic plants than in Col-0 plants under normal conditions (Figure 6d). Under drought conditions, expression of the Atrboh genes was further induced at least twofold in both Col-0 plants and 35S:4ΔC transgenic plants, showing that NTL4-mediated drought stress signals up-regulate a subset of the Atrboh genes
Lee S, Seo PJ, Lee HJ, Park CM - Accelerated cell death 2 suppresses mitochondrial oxidative bursts and modulates cell death in Arabidopsis
Visit the PubMed ArticleQuantitative real-time RT-PCR assays showed that the transcript levels of AtrbohA ... more than ninefold higher in 35S:4ΔC transgenic plants than in Col-0 plants under normal conditions (Figure 6d). Under drought conditions, expression of the Atrboh genes was further induced at least twofold in both Col-0 plants and 35S:4ΔC transgenic plants, showing that NTL4-mediated drought stress signals up-regulate a subset of the Atrboh genes
Lee S, Seo PJ, Lee HJ, Park CM - Accelerated cell death 2 suppresses mitochondrial oxidative bursts and modulates cell death in Arabidopsis
Visit the PubMed ArticleCell fractionation assays revealed that the NTL4 protein is mostly associated with microsomal fractions (Figure S7), consistent with localization of the NTL4 protein in the plasma membranes
Lee S, Seo PJ, Lee HJ, Park CM - Accelerated cell death 2 suppresses mitochondrial oxidative bursts and modulates cell death in Arabidopsis
Visit the PubMed ArticleThe transcript levels of other Atrboh genes, such as ... AtrbohD ... were slightly altered in 35S:4ΔC transgenic plants and the ntl4-1 mutant under both normal and drought conditions (Figure S6). However, the degrees of alterations were not significant, and the alteration patterns did not match the predicted role of NTL4 in drought stress responses, suggesting that these Atrboh genes are not directly related to NTL4
Lee S, Seo PJ, Lee HJ, Park CM - Accelerated cell death 2 suppresses mitochondrial oxidative bursts and modulates cell death in Arabidopsis
Visit the PubMed ArticleAtrbohE ... The inductive effects of drought on the Atrboh gene expression were diminished in the ntl4-1 mutant
Lee S, Seo PJ, Lee HJ, Park CM - Accelerated cell death 2 suppresses mitochondrial oxidative bursts and modulates cell death in Arabidopsis
Visit the PubMed ArticleExpression of the NTL4 gene was induced approximately threefold by ABA
Lee S, Seo PJ, Lee HJ, Park CM - Accelerated cell death 2 suppresses mitochondrial oxidative bursts and modulates cell death in Arabidopsis
Visit the PubMed ArticleWe found that the SAG12 gene is induced at earlier growth stages in 35S:4ΔC transgenic plants
Lee S, Seo PJ, Lee HJ, Park CM - Accelerated cell death 2 suppresses mitochondrial oxidative bursts and modulates cell death in Arabidopsis
Visit the PubMed ArticleTherefore, to explore the molecular phenotype of the rrc1 mutants, we examined whether the splicing patterns of the SR protein genes were affected in the ΔRS alleles (rrc1-2 and rrc1-3) and the null allele (rrc1-4) by RT-PCR analysis. Indeed, all of these rrc1 alleles displayed aberrant alternative splicing of several of the SR protein genes, i.e ... RS40
Shikata H, Shibata M, Ushijima T, Nakashima M, Kong SG, Matsuoka K, Lin C, Matsushita - The RS domain of Arabidopsis splicing factor RRC1 is required for phytochrome B signal transduction
Visit the PubMed ArticleTherefore, to explore the molecular phenotype of the rrc1 mutants, we examined whether the splicing patterns of the SR protein genes were affected in the ΔRS alleles (rrc1-2 and rrc1-3) and the null allele (rrc1-4) by RT-PCR analysis. Indeed, all of these rrc1 alleles displayed aberrant alternative splicing of several of the SR protein genes, i.e ... RS31
Shikata H, Shibata M, Ushijima T, Nakashima M, Kong SG, Matsuoka K, Lin C, Matsushita - The RS domain of Arabidopsis splicing factor RRC1 is required for phytochrome B signal transduction
Visit the PubMed ArticleRRC1 ... expressed in all of the organs examined
Shikata H, Shibata M, Ushijima T, Nakashima M, Kong SG, Matsuoka K, Lin C, Matsushita - The RS domain of Arabidopsis splicing factor RRC1 is required for phytochrome B signal transduction
Visit the PubMed ArticleTo link the molecular function of RRC1 to phyB signaling, we investigated whether phyB controls the alternative splicing of the SR protein genes that were affected in the rrc1 mutants. For each SR protein gene, we quantified the absolute mass of each splice variant in the RT-PCR analysis, and determined the time course of alterations in the ratio of splice variants to the total mass of the transcripts after transition from dark to cR conditions. We confirmed that the calculated ratio of splice variants in the same sample was constant over the number of PCR cycles used in this assay ... SR34b ... exhibited transient alterations in their splicing patterns in response to cR irradiation, and the response was significantly reduced in ... rrc1-3
Shikata H, Shibata M, Ushijima T, Nakashima M, Kong SG, Matsuoka K, Lin C, Matsushita - The RS domain of Arabidopsis splicing factor RRC1 is required for phytochrome B signal transduction
Visit the PubMed ArticleTherefore, to explore the molecular phenotype of the rrc1 mutants, we examined whether the splicing patterns of the SR protein genes were affected in the ΔRS alleles (rrc1-2 and rrc1-3) and the null allele (rrc1-4) by RT-PCR analysis. Indeed, all of these rrc1 alleles displayed aberrant alternative splicing of several of the SR protein genes, i.e ... SR34b
Shikata H, Shibata M, Ushijima T, Nakashima M, Kong SG, Matsuoka K, Lin C, Matsushita - The RS domain of Arabidopsis splicing factor RRC1 is required for phytochrome B signal transduction
Visit the PubMed Articlewe performed RT-PCR analysis on these PIF genes in the wild-type and rrc1 mutant plants, and found that the ratio of the minor variant mRNA-1 of PIF6 to the total amount of PIF6 transcript was increased in the rrc1 mutants (Figure S8). However, because it has been reported that overexpression of this minor variant of PIF6 enhances responses to cR in transgenic Arabidopsis plants (Penfield et al., 2010), it is unlikely that the splicing defects in PIF6 contribute to the reduced light responses in the rrc1 mutants
Shikata H, Shibata M, Ushijima T, Nakashima M, Kong SG, Matsuoka K, Lin C, Matsushita - The RS domain of Arabidopsis splicing factor RRC1 is required for phytochrome B signal transduction
Visit the PubMed ArticleTo link the molecular function of RRC1 to phyB signaling, we investigated whether phyB controls the alternative splicing of the SR protein genes that were affected in the rrc1 mutants. For each SR protein gene, we quantified the absolute mass of each splice variant in the RT-PCR analysis, and determined the time course of alterations in the ratio of splice variants to the total mass of the transcripts after transition from dark to cR conditions. We confirmed that the calculated ratio of splice variants in the same sample was constant over the number of PCR cycles used in this assay ... RS31 ... exhibited transient alterations in their splicing patterns in response to cR irradiation, and the response was significantly reduced in the phyB
Shikata H, Shibata M, Ushijima T, Nakashima M, Kong SG, Matsuoka K, Lin C, Matsushita - The RS domain of Arabidopsis splicing factor RRC1 is required for phytochrome B signal transduction
Visit the PubMed Articleby using a quantitative RT-PCR analysis, we examined the effects of the rrc1-2 or rrc1-3 mutations on the ability of the phyB molecule to induce the expression of four phyB-dependent, early-response genes ... The quantitative RT-PCR data showed that, in both of the rrc1 alleles ... DREB1A, exhibited a reduction in phyB-dependent, cR-induced expression
Shikata H, Shibata M, Ushijima T, Nakashima M, Kong SG, Matsuoka K, Lin C, Matsushita - The RS domain of Arabidopsis splicing factor RRC1 is required for phytochrome B signal transduction
Visit the PubMed ArticleTo link the molecular function of RRC1 to phyB signaling, we investigated whether phyB controls the alternative splicing of the SR protein genes that were affected in the rrc1 mutants. For each SR protein gene, we quantified the absolute mass of each splice variant in the RT-PCR analysis, and determined the time course of alterations in the ratio of splice variants to the total mass of the transcripts after transition from dark to cR conditions. We confirmed that the calculated ratio of splice variants in the same sample was constant over the number of PCR cycles used in this assay ... SR34b ... exhibited transient alterations in their splicing patterns in response to cR irradiation, and the response was significantly reduced in the phyB
Shikata H, Shibata M, Ushijima T, Nakashima M, Kong SG, Matsuoka K, Lin C, Matsushita - The RS domain of Arabidopsis splicing factor RRC1 is required for phytochrome B signal transduction
Visit the PubMed Articlein order to examine the intracellular localization of phyB, we introduced phyB-GFP into the rrc1-3 phyB-9 double mutant by crossing, and observed the resulting GFP fluorescence by confocal microscopy. As previously reported (Kircher et al., 1999; Yamaguchi et al., 1999), phyB-GFP accumulated in the nucleus and formed NBs under cW, not only in the phyB-9 single mutant but also in the rrc1-3 phyB-9 double mutant background (Figure 8a). These results indicate that the rrc1-3 mutation did not affect the subcellular localization of phyB under cW
Shikata H, Shibata M, Ushijima T, Nakashima M, Kong SG, Matsuoka K, Lin C, Matsushita - The RS domain of Arabidopsis splicing factor RRC1 is required for phytochrome B signal transduction
Visit the PubMed ArticleTo link the molecular function of RRC1 to phyB signaling, we investigated whether phyB controls the alternative splicing of the SR protein genes that were affected in the rrc1 mutants. For each SR protein gene, we quantified the absolute mass of each splice variant in the RT-PCR analysis, and determined the time course of alterations in the ratio of splice variants to the total mass of the transcripts after transition from dark to cR conditions. We confirmed that the calculated ratio of splice variants in the same sample was constant over the number of PCR cycles used in this assay ... RS31 ... exhibited transient alterations in their splicing patterns in response to cR irradiation, and the response was significantly reduced in ... rrc1-3
Shikata H, Shibata M, Ushijima T, Nakashima M, Kong SG, Matsuoka K, Lin C, Matsushita - The RS domain of Arabidopsis splicing factor RRC1 is required for phytochrome B signal transduction
Visit the PubMed ArticleWe used an anti-FLAG antibody to investigate the intracellular localization of RRC1-FLAG (Figure 2) that was overexpressed in the Arabidopsis wild-type background, and found that RRC1-FLAG localized to the nucleus and formed speckles
Shikata H, Shibata M, Ushijima T, Nakashima M, Kong SG, Matsuoka K, Lin C, Matsushita - The RS domain of Arabidopsis splicing factor RRC1 is required for phytochrome B signal transduction
Visit the PubMed Articleby using a quantitative RT-PCR analysis, we examined the effects of the rrc1-2 or rrc1-3 mutations on the ability of the phyB molecule to induce the expression of four phyB-dependent, early-response genes ... At5g25120 ... expression ... appeared to be up-regulated
Shikata H, Shibata M, Ushijima T, Nakashima M, Kong SG, Matsuoka K, Lin C, Matsushita - The RS domain of Arabidopsis splicing factor RRC1 is required for phytochrome B signal transduction
Visit the PubMed ArticleTherefore, to explore the molecular phenotype of the rrc1 mutants, we examined whether the splicing patterns of the SR protein genes were affected in the ΔRS alleles (rrc1-2 and rrc1-3) and the null allele (rrc1-4) by RT-PCR analysis. Indeed, all of these rrc1 alleles displayed aberrant alternative splicing of several of the SR protein genes, i.e. SR34
Shikata H, Shibata M, Ushijima T, Nakashima M, Kong SG, Matsuoka K, Lin C, Matsushita - The RS domain of Arabidopsis splicing factor RRC1 is required for phytochrome B signal transduction
Visit the PubMed ArticleWe investigated which step of phyB signal transduction was altered in the ΔRS alleles rrc1-2 and rrc1-3. We first sequenced the mRNA of PHYB in rrc1-2 and rrc1-3, and did not find any alterations compared with the wild-type sequence
Shikata H, Shibata M, Ushijima T, Nakashima M, Kong SG, Matsuoka K, Lin C, Matsushita - The RS domain of Arabidopsis splicing factor RRC1 is required for phytochrome B signal transduction
Visit the PubMed ArticleTo test the possibility that phyB and RRC1 function in the same subnuclear compartment, we examined their colocalization in transgenic Arabidopsis plants expressing both RRC1-FLAG and the fusion protein of phyB and GFP (phyB-GFP), which has been shown to be biologically functional in vivo (Yamaguchi et al., 1999). Although, under cR, a fraction of each fusion protein localized in the vicinity of the other in the nucleus, RRC1-FLAG speckles and phyB-GFP NBs were not completely overlapping, at least not under the conditions examined here
Shikata H, Shibata M, Ushijima T, Nakashima M, Kong SG, Matsuoka K, Lin C, Matsushita - The RS domain of Arabidopsis splicing factor RRC1 is required for phytochrome B signal transduction
Visit the PubMed ArticleAt5g10800 ... expressed in all of the organs examined
Shikata H, Shibata M, Ushijima T, Nakashima M, Kong SG, Matsuoka K, Lin C, Matsushita - The RS domain of Arabidopsis splicing factor RRC1 is required for phytochrome B signal transduction
Visit the PubMed ArticleTo test the possibility that phyB and RRC1 function in the same subnuclear compartment, we examined their colocalization in transgenic Arabidopsis plants expressing both RRC1-FLAG and the fusion protein of phyB and GFP (phyB-GFP), which has been shown to be biologically functional in vivo (Yamaguchi et al., 1999). Although, under cR, a fraction of each fusion protein localized in the vicinity of the other in the nucleus, RRC1-FLAG speckles and phyB-GFP NBs were not completely overlapping, at least not under the conditions examined here
Shikata H, Shibata M, Ushijima T, Nakashima M, Kong SG, Matsuoka K, Lin C, Matsushita - The RS domain of Arabidopsis splicing factor RRC1 is required for phytochrome B signal transduction
Visit the PubMed Articlewe also generated a transgenic line in the Arabidopsis wild-type background that expressed RRC1 fused to green fluorescent protein (GFP) (RRC1-GFP) under the control of the constitutive CaMV 35S promoter. Confocal microscopy analysis of GFP fluorescence confirmed that the protein localized to the nucleus and showed the speckled distribution
Shikata H, Shibata M, Ushijima T, Nakashima M, Kong SG, Matsuoka K, Lin C, Matsushita - The RS domain of Arabidopsis splicing factor RRC1 is required for phytochrome B signal transduction
Visit the PubMed Articleby using a quantitative RT-PCR analysis, we examined the effects of the rrc1-2 or rrc1-3 mutations on the ability of the phyB molecule to induce the expression of four phyB-dependent, early-response genes ... PRR9 ... expression ... appeared to be up-regulated
Shikata H, Shibata M, Ushijima T, Nakashima M, Kong SG, Matsuoka K, Lin C, Matsushita - The RS domain of Arabidopsis splicing factor RRC1 is required for phytochrome B signal transduction
Visit the PubMed ArticleTherefore, to explore the molecular phenotype of the rrc1 mutants, we examined whether the splicing patterns of the SR protein genes were affected in the ΔRS alleles (rrc1-2 and rrc1-3) and the null allele (rrc1-4) by RT-PCR analysis. Indeed, all of these rrc1 alleles displayed aberrant alternative splicing of several of the SR protein genes, i.e ... RS31a
Shikata H, Shibata M, Ushijima T, Nakashima M, Kong SG, Matsuoka K, Lin C, Matsushita - The RS domain of Arabidopsis splicing factor RRC1 is required for phytochrome B signal transduction
Visit the PubMed Articleby using a quantitative RT-PCR analysis, we examined the effects of the rrc1-2 or rrc1-3 mutations on the ability of the phyB molecule to induce the expression of four phyB-dependent, early-response genes ... PRR5 ... expression ... appeared to be up-regulated
Shikata H, Shibata M, Ushijima T, Nakashima M, Kong SG, Matsuoka K, Lin C, Matsushita - The RS domain of Arabidopsis splicing factor RRC1 is required for phytochrome B signal transduction
Visit the PubMed Articleevaluating the relative abundance of their mRNAs in wild-type and AACT2i-1 plants by quantitative RT-PCR ... expression of FPS1 (At5g47770 ... was reduced
Jin H, Song Z, Nikolau BJ - Reverse genetic characterization of two paralogous acetoacetyl CoA thiolase genes in Arabidopsis reveals their importance in plant growth and development
Visit the PubMed Articlemature organs, fully expanded leaves, mature siliques, flowers and stems of the first internode exhibited less AACT2 promoter-driven GUS activity
Jin H, Song Z, Nikolau BJ - Reverse genetic characterization of two paralogous acetoacetyl CoA thiolase genes in Arabidopsis reveals their importance in plant growth and development
Visit the PubMed ArticleAACT1 promoter-driven GUS expression is primarily concentrated in the vascular system of various organs ... including ... cotyledons, but not in young initiating leaves
Jin H, Song Z, Nikolau BJ - Reverse genetic characterization of two paralogous acetoacetyl CoA thiolase genes in Arabidopsis reveals their importance in plant growth and development
Visit the PubMed ArticleWestern blot analysis of extracts prepared from various organs demonstrated the accumulation pattern of the two AACT proteins (Figure 3). With the exception of roots, in which both proteins accumulate at similar levels, the expression level of AACT2 is considerably higher than that of AACT1 in leaves, stems, inflorescences and siliques
Jin H, Song Z, Nikolau BJ - Reverse genetic characterization of two paralogous acetoacetyl CoA thiolase genes in Arabidopsis reveals their importance in plant growth and development
Visit the PubMed Articleevaluating the relative abundance of their mRNAs in wild-type and AACT2i-1 plants by quantitative RT-PCR ... expression of HMG1 (At1g76490), which is considered a key controlling step this pathway, was not significantly altered
Jin H, Song Z, Nikolau BJ - Reverse genetic characterization of two paralogous acetoacetyl CoA thiolase genes in Arabidopsis reveals their importance in plant growth and development
Visit the PubMed ArticleWestern blot analysis of extracts prepared from various organs demonstrated the accumulation pattern of the two AACT proteins (Figure 3). With the exception of roots, in which both proteins accumulate at similar levels, the expression level of AACT2 is considerably higher than that of AACT1 in leaves, stems, inflorescences and siliques
Jin H, Song Z, Nikolau BJ - Reverse genetic characterization of two paralogous acetoacetyl CoA thiolase genes in Arabidopsis reveals their importance in plant growth and development
Visit the PubMed Articleevaluating the relative abundance of their mRNAs in wild-type and AACT2i-1 plants by quantitative RT-PCR ... expression of ... SMT2 (At1g20330 ... was reduced
Jin H, Song Z, Nikolau BJ - Reverse genetic characterization of two paralogous acetoacetyl CoA thiolase genes in Arabidopsis reveals their importance in plant growth and development
Visit the PubMed ArticleAACT1 promoter-driven ... GUS activity was clearly detected in the vascular system of young leaves, fully expanded leaves
Jin H, Song Z, Nikolau BJ - Reverse genetic characterization of two paralogous acetoacetyl CoA thiolase genes in Arabidopsis reveals their importance in plant growth and development
Visit the PubMed ArticleAACT2 promoter-driven ... GUS activity was observed in new emerging leaves of seedlings
Jin H, Song Z, Nikolau BJ - Reverse genetic characterization of two paralogous acetoacetyl CoA thiolase genes in Arabidopsis reveals their importance in plant growth and development
Visit the PubMed ArticleIn addition, the AACT2 protein level is decreased by 24% when plants are deprived of illumination for a 2-day period, compared with those maintained under constant illumination (Figure 3a)
Jin H, Song Z, Nikolau BJ - Reverse genetic characterization of two paralogous acetoacetyl CoA thiolase genes in Arabidopsis reveals their importance in plant growth and development
Visit the PubMed Articleevaluating the relative abundance of their mRNAs in wild-type and AACT2i-1 plants by quantitative RT-PCR ... expression of ... FPS2 (At4g17190 ... was reduced
Jin H, Song Z, Nikolau BJ - Reverse genetic characterization of two paralogous acetoacetyl CoA thiolase genes in Arabidopsis reveals their importance in plant growth and development
Visit the PubMed ArticleUsing gene-specific primers from SAUR19–24 UTR regions and RNA prepared from whole seedlings, our quantitative RT-PCR analysis detected a two- to three-fold up-regulation of all six SAUR19–24 family members following a 30-min auxin treatment (Figure 1). While auxin-inducible expression of the SAUR19–24 family members was relatively modest compared to the strongly auxin-inducible IAA5 gene, tissue-specific increases in expression were observed with SAUR19–24 promoter-GUS reporters in some instances
Spartz AK, Lee SH, Wenger JP, Gonzalez N, Itoh H, Inzé D, Peer WA, Murphy AS, Overvoorde PJ, Gray WM - The SAUR19 subfamily of SMALL AUXIN UP RNA genes promote cell expansion
Visit the PubMed ArticleUpon examining light-grown GFP–SAUR seedlings by confocal microscopy, we were surprised to find GFP fusions to SAUR ... 24 ... localized primarily to the cell surface, suggesting association with the plasma membrane
Spartz AK, Lee SH, Wenger JP, Gonzalez N, Itoh H, Inzé D, Peer WA, Murphy AS, Overvoorde PJ, Gray WM - The SAUR19 subfamily of SMALL AUXIN UP RNA genes promote cell expansion
Visit the PubMed ArticleSAUR19 localizes primarily to the plasma membrane. Confocal images of 35S:GFP–SAUR19 fluorescence in ... leaf epidermal cells
Spartz AK, Lee SH, Wenger JP, Gonzalez N, Itoh H, Inzé D, Peer WA, Murphy AS, Overvoorde PJ, Gray WM - The SAUR19 subfamily of SMALL AUXIN UP RNA genes promote cell expansion
Visit the PubMed ArticleUsing gene-specific primers from SAUR19–24 UTR regions and RNA prepared from whole seedlings, our quantitative RT-PCR analysis detected a two- to three-fold up-regulation of all six SAUR19–24 family members following a 30-min auxin treatment (Figure 1). While auxin-inducible expression of the SAUR19–24 family members was relatively modest compared to the strongly auxin-inducible IAA5 gene, tissue-specific increases in expression were observed with SAUR19–24 promoter-GUS reporters in some instances
Spartz AK, Lee SH, Wenger JP, Gonzalez N, Itoh H, Inzé D, Peer WA, Murphy AS, Overvoorde PJ, Gray WM - The SAUR19 subfamily of SMALL AUXIN UP RNA genes promote cell expansion
Visit the PubMed ArticleUpon examining light-grown GFP–SAUR seedlings by confocal microscopy, we were surprised to find GFP fusions to SAUR ... 21 ... localized primarily to the cell surface, suggesting association with the plasma membrane
Spartz AK, Lee SH, Wenger JP, Gonzalez N, Itoh H, Inzé D, Peer WA, Murphy AS, Overvoorde PJ, Gray WM - The SAUR19 subfamily of SMALL AUXIN UP RNA genes promote cell expansion
Visit the PubMed ArticleTo examine auxin-induced gene expression, we crossed the DR5-GUS reporter into plants harboring the GFP–SAUR19 transgene. While the overall pattern of GUS expression was not noticeably altered by the expression of the SAUR transgene, we consistently detected a slight reduction in the intensity of DR5-GUS staining following short inductions with auxin
Spartz AK, Lee SH, Wenger JP, Gonzalez N, Itoh H, Inzé D, Peer WA, Murphy AS, Overvoorde PJ, Gray WM - The SAUR19 subfamily of SMALL AUXIN UP RNA genes promote cell expansion
Visit the PubMed ArticleMicrosomal fractionation studies confirmed that the GFP–SAUR19 protein is membrane associated (Figure 7d). To confirm that the membrane association observed with the GFP–SAUR19 fusion protein was not an artifact of the GFP tag, we examined the fractionation of untagged SAUR19 as well as the StrepII–SAUR19 fusion protein. Consistent with our GFP–SAUR19 findings, the untagged and StrepII-tagged SAUR19 derivatives were also highly enriched in microsomal pellets (Figure 7d). To confirm that membrane association was with plasma membranes as suggested by our microscopy studies, microsomal fractions were subjected to two-phase partitioning assays. The GFP–SAUR19 protein was highly enriched in plasma membrane-enriched fractions, whereas the endoplasmic reticulum-associated SEC12 protein was found predominantly in the ‘other membrane’ fraction (Figure 7e). Consistent with the lack of a predicted transmembrane spanning domain in the SAUR19 sequence, GFP–SAUR19 could be extracted from membrane fractions with high-pH buffer, indicating that SAUR19 is a peripheral rather than an integral membrane protein
Spartz AK, Lee SH, Wenger JP, Gonzalez N, Itoh H, Inzé D, Peer WA, Murphy AS, Overvoorde PJ, Gray WM - The SAUR19 subfamily of SMALL AUXIN UP RNA genes promote cell expansion
Visit the PubMed ArticleUpon examining light-grown GFP–SAUR seedlings by confocal microscopy, we were surprised to find GFP fusions to SAUR ... 23 ... localized primarily to the cell surface, suggesting association with the plasma membrane
Spartz AK, Lee SH, Wenger JP, Gonzalez N, Itoh H, Inzé D, Peer WA, Murphy AS, Overvoorde PJ, Gray WM - The SAUR19 subfamily of SMALL AUXIN UP RNA genes promote cell expansion
Visit the PubMed ArticleUsing gene-specific primers from SAUR19–24 UTR regions and RNA prepared from whole seedlings, our quantitative RT-PCR analysis detected a two- to three-fold up-regulation of all six SAUR19–24 family members following a 30-min auxin treatment (Figure 1). While auxin-inducible expression of the SAUR19–24 family members was relatively modest compared to the strongly auxin-inducible IAA5 gene, tissue-specific increases in expression were observed with SAUR19–24 promoter-GUS reporters in some instances
Spartz AK, Lee SH, Wenger JP, Gonzalez N, Itoh H, Inzé D, Peer WA, Murphy AS, Overvoorde PJ, Gray WM - The SAUR19 subfamily of SMALL AUXIN UP RNA genes promote cell expansion
Visit the PubMed ArticleSAUR19 ... rapidly induced by auxin ... in wild-type seedlings following a 30-min treatment with 0.35 μm indole-3-acetic acid (IAA
Spartz AK, Lee SH, Wenger JP, Gonzalez N, Itoh H, Inzé D, Peer WA, Murphy AS, Overvoorde PJ, Gray WM - The SAUR19 subfamily of SMALL AUXIN UP RNA genes promote cell expansion
Visit the PubMed ArticleUsing gene-specific primers from SAUR19–24 UTR regions and RNA prepared from whole seedlings, our quantitative RT-PCR analysis detected a two- to three-fold up-regulation of all six SAUR19–24 family members following a 30-min auxin treatment (Figure 1). While auxin-inducible expression of the SAUR19–24 family members was relatively modest compared to the strongly auxin-inducible IAA5 gene, tissue-specific increases in expression were observed with SAUR19–24 promoter-GUS reporters in some instances
Spartz AK, Lee SH, Wenger JP, Gonzalez N, Itoh H, Inzé D, Peer WA, Murphy AS, Overvoorde PJ, Gray WM - The SAUR19 subfamily of SMALL AUXIN UP RNA genes promote cell expansion
Visit the PubMed ArticleUsing gene-specific primers from SAUR19–24 UTR regions and RNA prepared from whole seedlings, our quantitative RT-PCR analysis detected a two- to three-fold up-regulation of all six SAUR19–24 family members following a 30-min auxin treatment (Figure 1). While auxin-inducible expression of the SAUR19–24 family members was relatively modest compared to the strongly auxin-inducible IAA5 gene, tissue-specific increases in expression were observed with SAUR19–24 promoter-GUS reporters in some instances
Spartz AK, Lee SH, Wenger JP, Gonzalez N, Itoh H, Inzé D, Peer WA, Murphy AS, Overvoorde PJ, Gray WM - The SAUR19 subfamily of SMALL AUXIN UP RNA genes promote cell expansion
Visit the PubMed ArticleUsing gene-specific primers from SAUR19–24 UTR regions and RNA prepared from whole seedlings, our quantitative RT-PCR analysis detected a two- to three-fold up-regulation of all six SAUR19–24 family members following a 30-min auxin treatment (Figure 1). While auxin-inducible expression of the SAUR19–24 family members was relatively modest compared to the strongly auxin-inducible IAA5 gene, tissue-specific increases in expression were observed with SAUR19–24 promoter-GUS reporters in some instances
Spartz AK, Lee SH, Wenger JP, Gonzalez N, Itoh H, Inzé D, Peer WA, Murphy AS, Overvoorde PJ, Gray WM - The SAUR19 subfamily of SMALL AUXIN UP RNA genes promote cell expansion
Visit the PubMed ArticleUpon examining light-grown GFP–SAUR seedlings by confocal microscopy, we were surprised to find GFP fusions to SAUR19 ... localized primarily to the cell surface, suggesting association with the plasma membrane
Spartz AK, Lee SH, Wenger JP, Gonzalez N, Itoh H, Inzé D, Peer WA, Murphy AS, Overvoorde PJ, Gray WM - The SAUR19 subfamily of SMALL AUXIN UP RNA genes promote cell expansion
Visit the PubMed ArticleQuantitative RT-PCR analysis revealed down-regulation of ROX mRNA levels in vegetative las-4 shoot apices compared to wild-type
Yang F, Wang Q, Schmitz G, Müller D, Theres K - The bHLH protein ROX acts in concert with RAX1 and LAS to modulate axillary meristem formation in Arabidopsis
Visit the PubMed Articlemonitoring of ROX transcript accumulation in rax1 mutant backgrounds revealed strong down-regulation during the vegetative stage of development. A total of 28 rax1 mutant (rax1-3 or rax1-3 rax2-1 rax3-1) apices were analyzed in nine independent in situ hybridization experiments. Strong ROX hybridization signals were observed in sections from Col wild-type apices, but ROX transcripts were not detectable in most rax1-3 and rax1-3 rax2-1 rax3-1 mutant apices (Figure 6e,g). Only on four sections from three apices of rax1-3 plants and rax1-3 rax2-1 rax3-1 triple mutants were very faint hybridization signals found in the leaf axils
Yang F, Wang Q, Schmitz G, Müller D, Theres K - The bHLH protein ROX acts in concert with RAX1 and LAS to modulate axillary meristem formation in Arabidopsis
Visit the PubMed ArticleIn situ hybridization experiments detected ROX transcripts in the expected domains in sections of vegetative apices of the Col wild-type (Figure 6a), but they were barely detectable in vegetative las-4 apices ... This result was verified by hybridizing comparable sections from wild-type and las-4 apices side by side on the same slide. A total of eight independent hybridizations with 16 individual las-4 shoot apices did not reveal a ROX hybridization signal, except in two sections from one apex, which showed a faint signal
Yang F, Wang Q, Schmitz G, Müller D, Theres K - The bHLH protein ROX acts in concert with RAX1 and LAS to modulate axillary meristem formation in Arabidopsis
Visit the PubMed ArticleTo investigate the expression pattern of ROX, we performed RNA in situ hybridizations on tissue sections from Col wild-type plants ... Transverse sections (Figure 4f–j) revealed that ROX mRNA accumulated in a circular domain at the center of the boundary region between the shoot apical meristem and young leaf primordia
Yang F, Wang Q, Schmitz G, Müller D, Theres K - The bHLH protein ROX acts in concert with RAX1 and LAS to modulate axillary meristem formation in Arabidopsis
Visit the PubMed ArticleThe pattern of LAS transcript accumulation in vegetative rox-1 shoot apices was similar to that in the wild-type
Yang F, Wang Q, Schmitz G, Müller D, Theres K - The bHLH protein ROX acts in concert with RAX1 and LAS to modulate axillary meristem formation in Arabidopsis
Visit the PubMed Articlethe cellular distribution of RAX1 transcripts in vegetative rox-1 shoot apices was comparable to that observed in the Col wild-type
Yang F, Wang Q, Schmitz G, Müller D, Theres K - The bHLH protein ROX acts in concert with RAX1 and LAS to modulate axillary meristem formation in Arabidopsis
Visit the PubMed ArticleTo investigate the expression pattern of ROX, we performed RNA in situ hybridizations on tissue sections from Col wild-type plants ... During vegetative development, ROX transcripts accumulated at the adaxial boundary of leaf primordia, including cells in the L3 layer and below, and frequently excluding cells of the L1 layer, or L1 and L2 layers, of the shoot apex
Yang F, Wang Q, Schmitz G, Müller D, Theres K - The bHLH protein ROX acts in concert with RAX1 and LAS to modulate axillary meristem formation in Arabidopsis
Visit the PubMed ArticleTo investigate the expression pattern of ROX, we performed RNA in situ hybridizations on tissue sections from Col wild-type plants ... ROX mRNA was transiently detected from P0 to approximately P10/P13, before the first indication of AM formation
Yang F, Wang Q, Schmitz G, Müller D, Theres K - The bHLH protein ROX acts in concert with RAX1 and LAS to modulate axillary meristem formation in Arabidopsis
Visit the PubMed ArticleA comparable reduction of ROX transcript accumulation was observed in vegetative apices of rax1-3 las-4 double mutants
Yang F, Wang Q, Schmitz G, Müller D, Theres K - The bHLH protein ROX acts in concert with RAX1 and LAS to modulate axillary meristem formation in Arabidopsis
Visit the PubMed ArticleSimilar to FILpro:WOX1 transgenic plants, the kan1 kan2 double mutant has been reported to have adventitious outgrowths and ectopic margin-like structures on the abaxial sides of leaves (Eshed et al., 2001, 2004). However, it has been reported that KAN1 expression is not significantly altered in prs wox1 (Vandenbussche et al., 2009), and we found that the expression of KAN1 and KAN2 did not decrease in FILpro:WOX1 (Figure 2H), suggesting that PRS and WOX1 do not repress KAN expression. Therefore, we investigated whether the expression of PRS and/or WOX1 is affected in kan1 kan2. The expression levels of PRS and WOX1 in kan1 kan2 were approximately twofold and fivefold of those of the wild type, respectively (Figure 6A
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleThe HD-ZIPIII family genes are adaxially expressed in leaf primordia (McConnell et al., 2001). The microRNA miR165/166 is reported to repress the expression of the HD-ZIPIII family genes (Mallory et al., 2004). To investigate whether mutations of the WOX genes affect the pattern of HD-ZIPIII expression via the regulation of miR165/166, we analyzed the expression pattern of the 35Spro:miYFP-W line, in which miR165/166 function can be monitored by a reduction of YFP fluorescence (Toyokura et al., 2011). YFP fluorescence of 35Spro:miYFP-W in the wild type was detected only in the two cell layers of the adaxial side of the leaf primordia (Figure 5C). In prs wox1, YFP fluorescence was further limited to the medial region of the adaxial side (Figure 5C
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleNext, to understand better the roles of PRS and WOX1 in margin development, we examined expression patterns in the KLUH (KLU)pro:vYFPer line (vYFPer is an endoplasmic reticulum–localized VENUS–YFP ... In FILpro:WOX1, the YFP signal was observed in the abaxial region of leaf primordia (Figure 3H; see Supplemental Figure 5A online), including the abaxial outgrowths (Supplemental Figures 5A and 5C online), in addition to the leaf base and margin
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleNext, we analyzed whether PRS and WOX1 affect the expression of the adaxial- and abaxial-specific genes. AS2 expression in wild-type leaf primordia was detected in adaxial epidermal cells and rarely in adaxial subepidermal cells but was not detected at the margin and on the abaxial side
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed Articlein situ hybridization ... ISH) targeting HISTONE H4 ... prs wox1 leaf primordia ... were significantly fewer than those in wild-type leaf primordia
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleConsistent with the distribution of the trichomes, AS2 expression was detected only on the adaxial side in the kan1 kan2 leaf primordia (Figure 7G) but in the entire region of the epidermis in the prs wox1 kan1 kan2 leaf primordia (Figure 7H
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleNo WOX1 signal was detected in ... P1-P2 leaf ... primordia
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleNext, to understand better the roles of PRS and WOX1 in margin development, we examined expression patterns in the KLUH (KLU)pro:vYFPer line (vYFPer is an endoplasmic reticulum–localized VENUS–YFP ... In FILpro:WOX1, the YFP signal was observed in the abaxial region of leaf primordia (Figure 3H; see Supplemental Figure 5A online), including the abaxial outgrowths (Supplemental Figures 5A and 5C online
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleWe also found PRS expression in the two middle mesophyll layers of the blade in P4-P6 leaf primordia
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleNext, to understand better the roles of PRS and WOX1 in margin development, we examined expression patterns in the KLUH (KLU)pro:vYFPer line (vYFPer is an endoplasmic reticulum–localized VENUS–YFP ... The KLUpro:vYFPer signal was absent from the margin of the leaf blade in the prs wox1 background (Figure 3H; see Supplemental Figure 5A online) but persisted in the leaf base
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleThe AS2 expression pattern in prs wox1 was indistinguishable from that of the wild type in P3 leaf primordia, but ectopic expression was detected in the margin cells of P4-P6 leaf primordia and in abaxial epidermal cells of the lateral leaf regions after the P7 stage
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleThe abaxial-specific gene FIL is expressed in P3-P6 leaf primordia of the wild type in the broad region of the abaxial side, including the margin and four cell layers of the six-cell-layered blade on the abaxial side (Figure 5A). After the P7 stage, FIL expression was restricted to three cell layers of the abaxial side (Figure 5A
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleIn P3 leaf primordia, WOX1 expression was detected in a few cells located in an area spanning the leaf edge and approximately three cell layers deep
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleThe FIL expression in the prs wox1 leaf primordia was indistinguishable from that in the wild type at the P3 stage, but unlike the wild type, it was detected in a broader region of P4-P6 leaf primordia, including the lateral region of the adaxial side (Figure 5A). After the P7 stage, FIL expression was restricted to three cell layers of the blade, resembling the wild-type expression pattern, but it was also detected in the adaxial epidermis neighboring the margin (Figure 5A
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleIn addition, the expression of FIL was restricted to the abaxial region of the as2-1 leaf primordia (Figure 8H) but was detected in the entire region of the prs wox1 as2-1 leaf primordia (Figure 8I), indicating that the filamentous leaves of prs wox1 as2 are severely abaxialized
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleNext, to understand better the roles of PRS and WOX1 in margin development, we examined expression patterns in the KLUH (KLU)pro:vYFPer line (vYFPer is an endoplasmic reticulum–localized VENUS–YFP ... In FILpro:WOX1, the YFP signal was observed in the abaxial region of leaf primordia (Figure 3H; see Supplemental Figure 5A online), including the abaxial outgrowths (Supplemental Figures 5A and 5C online), in addition to the leaf base
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleThe HD-ZIPIII family genes are adaxially expressed in leaf primordia (McConnell et al., 2001). The microRNA miR165/166 is reported to repress the expression of the HD-ZIPIII family genes (Mallory et al., 2004). To investigate whether mutations of the WOX genes affect the pattern of HD-ZIPIII expression via the regulation of miR165/166, we analyzed the expression pattern of the 35Spro:miYFP-W line, in which miR165/166 function can be monitored by a reduction of YFP fluorescence (Toyokura et al., 2011). YFP fluorescence of 35Spro:miYFP-W in the wild type was detected only in the two cell layers of the adaxial side of the leaf primordia (Figure 5C
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleISH analyses revealed the expanded accumulation of PRS and WOX1 transcripts in the abaxial region of the kan1 kan2 leaf primordia (Figures 6B and 6C
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleThe FIL expression in the prs wox1 leaf primordia was indistinguishable from that in the wild type at the P3 stage, but unlike the wild type, it was detected in a broader region of P4-P6 leaf primordia, including the lateral region of the adaxial side (Figure 5A). After the P7 stage, FIL expression was restricted to three cell layers of the blade, resembling the wild-type expression pattern, but it was also detected in the adaxial epidermis neighboring the margin (Figure 5A
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticlePRS was expressed in the margin cells of the leaf primordia beginning at the P1 stage
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed Articlein prs wox1 ... the frequency of H4-positive cells in the lateral region decreased to the same level as in the medial region
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleIn P3 leaf primordia ... expression was ... detected in a few cells located in an area spanning the leaf edge and approximately three cell layers deep ... similar to PRS ... expression
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleThe AS2 expression pattern in prs wox1 was indistinguishable from that of the wild type in P3 leaf primordia, but ectopic expression was detected in the margin cells of P4-P6 leaf primordia and in abaxial epidermal cells of the lateral leaf regions after the P7 stage ...
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleTo investigate whether the KAN1 protein directly or indirectly represses PRS and WOX1, we analyzed the effect of dexamethasone (DEX)–inducible expression of a 35Spro:KAN1:glucocorticoid receptor (GR) line, the hormone binding domain of the GR fused to the C terminus of KAN1 under the control of the 35S promoter (Hawker and Bowman, 2004), on the expression of PRS and WOX1. Exposure to DEX for 6 h drastically reduced the amounts of both PRS and WOX1 transcripts
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleWe found that WOX1 mRNA levels in FILpro:WOX1 plants with aberrant leaves increased to approximately fourfold or fivefold of those of the wild-type or FILpro:GUS control plants
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleNext, to understand better the roles of PRS and WOX1 in margin development, we examined expression patterns in the KLUH (KLU)pro:vYFPer line (vYFPer is an endoplasmic reticulum–localized VENUS–YFP) (Adamski et al., 2009) as a margin marker. The leaf primordia of KLUpro:vYFPer plants showed YFP fluorescence at the blade margins (Figure 3H; see Supplemental Figure 5A online) and leaf bases
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleIn addition, the expression of FIL was restricted to the abaxial region of the as2-1 leaf primordia (Figure 8H) but was detected in the entire region of the prs wox1 as2-1 leaf primordia (Figure 8I), indicating that the filamentous leaves of prs wox1 as2 are severely abaxialized
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleTo reveal whether FIL/YAB genes upregulate the expression of PRS and WOX1, we examined the expression levels of PRS and WOX1 in fil yab3 and fil yab3 yab5. The expression level of WOX1 in these mutants was approximately half of that in the wild type, whereas the expression level of PRS in the mutants was the same as in the wild type (Figure 6A
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleNo WOX1 signal was detected in the SAM
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleTo investigate whether the KAN1 protein directly or indirectly represses PRS and WOX1, we analyzed the effect of dexamethasone (DEX)–inducible expression of a 35Spro:KAN1:glucocorticoid receptor (GR) line, the hormone binding domain of the GR fused to the C terminus of KAN1 under the control of the 35S promoter (Hawker and Bowman, 2004), on the expression of PRS and WOX1. Exposure to DEX for 6 h drastically reduced the amounts of both PRS and WOX1 transcripts
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleIn P3 leaf primordia, KAN1 expression was restricted to a narrow region on the abaxial side and was absent from the cells neighboring the leaf edge (Figure 6D ... After the P4 stage, KAN1 signal was detected in two cell layers of the six-cell-layered region on the abaxial side (Figure 6D
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleSimilar to FILpro:WOX1 transgenic plants, the kan1 kan2 double mutant has been reported to have adventitious outgrowths and ectopic margin-like structures on the abaxial sides of leaves (Eshed et al., 2001, 2004). However, it has been reported that KAN1 expression is not significantly altered in prs wox1 (Vandenbussche et al., 2009), and we found that the expression of KAN1 and KAN2 did not decrease in FILpro:WOX1 (Figure 2H), suggesting that PRS and WOX1 do not repress KAN expression. Therefore, we investigated whether the expression of PRS and/or WOX1 is affected in kan1 kan2. The expression levels of PRS and WOX1 in kan1 kan2 were approximately twofold and fivefold of those of the wild type, respectively (Figure 6A
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleNext, to understand better the roles of PRS and WOX1 in margin development, we examined expression patterns in the KLUH (KLU)pro:vYFPer line (vYFPer is an endoplasmic reticulum–localized VENUS–YFP ... The KLUpro:vYFPer signal was absent from the margin of the leaf blade in the prs wox1 background
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleAfter the P4 stage, strong WOX1 expression was detected in the two middle mesophyll layers of the six-cell-layered blade region and in the margin cells
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleTo investigate whether the KAN1 protein directly or indirectly represses PRS and WOX1, we analyzed the effect of dexamethasone (DEX)–inducible expression of a 35Spro:KAN1:glucocorticoid receptor (GR) line, the hormone binding domain of the GR fused to the C terminus of KAN1 under the control of the 35S promoter (Hawker and Bowman, 2004), on the expression of PRS and WOX1 ... In contrast with WOX1, following exposure to DEX with CHX, PRS expression levels were only slightly reduced
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleISH analyses revealed the expanded accumulation of PRS and WOX1 transcripts in the abaxial region of the kan1 kan2 leaf primordia (Figures 6B and 6C
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleThe expression levels of abaxial-specific genes (FIL, KAN1, and KAN2) in the FILpro:WOX1 lines were comparable to those in the wild-type or FILpro:GUS lines
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleThe FIL expression in the prs wox1 leaf primordia was indistinguishable from that in the wild type at the P3 stage, but unlike the wild type, it was detected in a broader region of P4-P6 leaf primordia, including the lateral region of the adaxial side (Figure 5A). After the P7 stage, FIL expression was restricted to three cell layers of the blade, resembling the wild-type expression pattern, but it was also detected in the adaxial epidermis neighboring the margin (Figure 5A
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleThe expression levels of abaxial-specific genes (FIL, KAN1, and KAN2) in the FILpro:WOX1 lines were comparable to those in the wild-type or FILpro:GUS lines
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleTo investigate whether the KAN1 protein directly or indirectly represses PRS and WOX1, we analyzed the effect of dexamethasone (DEX)–inducible expression of a 35Spro:KAN1:glucocorticoid receptor (GR) line, the hormone binding domain of the GR fused to the C terminus of KAN1 under the control of the 35S promoter (Hawker and Bowman, 2004), on the expression of PRS and WOX1 ... Exposure to DEX in the presence of cycloheximide (CHX; an inhibitor of protein biosynthesis) led to a substantial reduction in the expression levels of WOX1, although the repression was stronger in the absence of CHX
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleThe abaxial-specific gene FIL is expressed in P3-P6 leaf primordia of the wild type in the broad region of the abaxial side, including the margin and four cell layers of the six-cell-layered blade on the abaxial side (Figure 5A). After the P7 stage, FIL expression was restricted to three cell layers of the abaxial side (Figure 5A
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleNext, to understand better the roles of PRS and WOX1 in margin development, we examined expression patterns in the KLUH (KLU)pro:vYFPer line (vYFPer is an endoplasmic reticulum–localized VENUS–YFP) (Adamski et al., 2009) as a margin marker. The leaf primordia of KLUpro:vYFPer plants showed YFP fluorescence at the blade margins
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleThe AS2 expression pattern in prs wox1 was indistinguishable from that of the wild type in P3 leaf primordia, but ectopic expression was detected in the margin cells of P4-P6 leaf primordia and in abaxial epidermal cells of the lateral leaf regions after the P7 stage
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleThe expression levels of abaxial-specific genes (FIL, KAN1, and KAN2) in the FILpro:WOX1 lines were comparable to those in the wild-type or FILpro:GUS lines
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleTo reveal whether FIL/YAB genes upregulate the expression of PRS and WOX1, we examined the expression levels of PRS and WOX1 in fil yab3 and fil yab3 yab5. The expression level of WOX1 in these mutants was approximately half of that in the wild type, whereas the expression level of PRS in the mutants was the same as in the wild type (Figure 6A
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleNext, we analyzed whether PRS and WOX1 affect the expression of the adaxial- and abaxial-specific genes. AS2 expression in wild-type leaf primordia was detected in adaxial epidermal cells and rarely in adaxial subepidermal cells but was not detected at the margin and on the abaxial side
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleTo reveal whether FIL/YAB genes upregulate the expression of PRS and WOX1, we examined the expression levels of PRS and WOX1 in fil yab3 and fil yab3 yab5. The expression level of WOX1 in these mutants was approximately half of that in the wild type, whereas the expression level of PRS in the mutants was the same as in the wild type (Figure 6A
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleTo reveal whether FIL/YAB genes upregulate the expression of PRS and WOX1, we examined the expression levels of PRS and WOX1 in fil yab3 and fil yab3 yab5. The expression level of WOX1 in these mutants was approximately half of that in the wild type, whereas the expression level of PRS in the mutants was the same as in the wild type (Figure 6A
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleConsistent with the distribution of the trichomes, AS2 expression was detected only on the adaxial side in the kan1 kan2 leaf primordia (Figure 7G) but in the entire region of the epidermis in the prs wox1 kan1 kan2 leaf primordia (Figure 7H
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleWe also analyzed the phenotypes of the FILpro:PRS plants ... despite the increased ... expression of PRS
Nakata M, Matsumoto N, Tsugeki R, Rikirsch E, Laux T, Okada K - Roles of the middle domain-specific WUSCHEL-RELATED HOMEOBOX genes in early development of leaves in Arabidopsis
Visit the PubMed ArticleTo further examine the spatiotemporal expression pattern of BP, we introduced a reporter construct composed of the promoter region of BP and the cDNA encoding the GUS reporter ... The level of GUS expression was analyzed by measurement of GUS histochemical activity ... Consistent with a previous report (Ori et al., 2000), GUS activity was detected in petioles or the proximal part of midveins in as2-1 leaves
Li Z, Li B, Shen WH, Huang H, Dong A - TCP transcription factors interact with AS2 in the repression of class-I KNOX genes in Arabidopsis thaliana
Visit the PubMed Articletranscript levels of BP ... were ... elevated in the ... jaw-D single mutant leaves
Li Z, Li B, Shen WH, Huang H, Dong A - TCP transcription factors interact with AS2 in the repression of class-I KNOX genes in Arabidopsis thaliana
Visit the PubMed Articletranscript levels of ... KNAT6 were ... further increased in the as2-1 jaw-D double mutant rosette ... leaves
Li Z, Li B, Shen WH, Huang H, Dong A - TCP transcription factors interact with AS2 in the repression of class-I KNOX genes in Arabidopsis thaliana
Visit the PubMed Articletranscript levels of ... KNAT2 ... were ... further increased in the as2-1 jaw-D double mutant rosette ... leaves
Li Z, Li B, Shen WH, Huang H, Dong A - TCP transcription factors interact with AS2 in the repression of class-I KNOX genes in Arabidopsis thaliana
Visit the PubMed Articletranscript levels of ... KNAT6 were ... elevated in the as2-1 ... single mutant leaves
Li Z, Li B, Shen WH, Huang H, Dong A - TCP transcription factors interact with AS2 in the repression of class-I KNOX genes in Arabidopsis thaliana
Visit the PubMed Articletranscript levels of ... KNAT2 ... were ... elevated in the ... jaw-D single mutant leaves
Li Z, Li B, Shen WH, Huang H, Dong A - TCP transcription factors interact with AS2 in the repression of class-I KNOX genes in Arabidopsis thaliana
Visit the PubMed Articletranscript levels of ... KNAT2 ... were ... elevated in the as2-1 ... mutant leaves
Li Z, Li B, Shen WH, Huang H, Dong A - TCP transcription factors interact with AS2 in the repression of class-I KNOX genes in Arabidopsis thaliana
Visit the PubMed ArticleTo further examine the spatiotemporal expression pattern of BP, we introduced a reporter construct composed of the promoter region of BP and the cDNA encoding the GUS reporter ... The level of GUS expression was analyzed by measurement of GUS histochemical activity ... in as2-1 jaw-D plants GUS activity was expanded to more distal regions, and the stronger GUS staining was visible in the as2-1 jaw-D double mutant than in the as2-1 single mutant leaves (Figure 4e). In addition, the apex of the leaf protrusions (Figure 4f) and the junction between the leaf and ectopic shoot (Figure 4g) were also stained deeply in the double mutant
Li Z, Li B, Shen WH, Huang H, Dong A - TCP transcription factors interact with AS2 in the repression of class-I KNOX genes in Arabidopsis thaliana
Visit the PubMed ArticleTo further examine the spatiotemporal expression pattern of BP, we introduced a reporter construct composed of the promoter region of BP and the cDNA encoding the GUS reporter ... The level of GUS expression was analyzed by measurement of GUS histochemical activity ... GUS activity was also barely detected in the jaw-D leaves
Li Z, Li B, Shen WH, Huang H, Dong A - TCP transcription factors interact with AS2 in the repression of class-I KNOX genes in Arabidopsis thaliana
Visit the PubMed Articletranscript levels of BP ... were ... elevated in the as2-1 ... mutant leaves
Li Z, Li B, Shen WH, Huang H, Dong A - TCP transcription factors interact with AS2 in the repression of class-I KNOX genes in Arabidopsis thaliana
Visit the PubMed Articletranscript levels of ... KNAT6 were ... elevated in the ... jaw-D single mutant leaves
Li Z, Li B, Shen WH, Huang H, Dong A - TCP transcription factors interact with AS2 in the repression of class-I KNOX genes in Arabidopsis thaliana
Visit the PubMed Articletranscript levels of BP ... were ... further increased in the as2-1 jaw-D double mutant rosette ... leaves
Li Z, Li B, Shen WH, Huang H, Dong A - TCP transcription factors interact with AS2 in the repression of class-I KNOX genes in Arabidopsis thaliana
Visit the PubMed ArticleConsistent with the real-time RT-PCR results, we could not detect CLV3 mRNA in meristems of 7-day-old ant-4 ail6-2 ail7-1 seedlings
Mudunkothge JS, Krizek BA - Three Arabidopsis AIL/PLT genes act in combination to regulate shoot apical meristem function
Visit the PubMed ArticleSTM mRNA was also detected in developing leaf primordia in the triple mutant, something not observed in the wild type (Figure 3g,h). The slightly increased levels of STM mRNA detected in ant-4 ail6-2 ail7-1 seedlings by real-time RT-PCR probably reflect this ectopic STM expression in developing leaves
Mudunkothge JS, Krizek BA - Three Arabidopsis AIL/PLT genes act in combination to regulate shoot apical meristem function
Visit the PubMed ArticleSTM mRNA was detected throughout the smaller ant-4 ail6-2 ail7-1 shoot apical meristems, unlike wild-type meristems, which show downregulation of STM expression in incipient lateral organ primordia
Mudunkothge JS, Krizek BA - Three Arabidopsis AIL/PLT genes act in combination to regulate shoot apical meristem function
Visit the PubMed ArticleTo investigate whether the meristem defects of ant-4 ail6-2 ail7-1 plants are associated with alterations in cell division, we examined the expression of the S phase marker histone H4. Seven-day-old ant-4 ail6-2 ail7-1 meristems had fewer histone H4-expressing cells relative to the wild type (Figure 2i–l). In 10-day-old seedlings, half of the triple mutant meristems examined had no histone H4-expressing cells while the remaining meristems had between one and three such cells
Mudunkothge JS, Krizek BA - Three Arabidopsis AIL/PLT genes act in combination to regulate shoot apical meristem function
Visit the PubMed ArticleSeven-day-old ant-4 ail6-2 ail7-1 ... CDKB2;2 expression was reduced ... compared with the wild type
Mudunkothge JS, Krizek BA - Three Arabidopsis AIL/PLT genes act in combination to regulate shoot apical meristem function
Visit the PubMed Articlein ant-4 ail6-2 ail7-1 7-day-old seedlings ... STM mRNA levels were slightly increased
Mudunkothge JS, Krizek BA - Three Arabidopsis AIL/PLT genes act in combination to regulate shoot apical meristem function
Visit the PubMed ArticleSeven-day-old ant-4 ail6-2 ail7-1 ... CDKB2;1 ... expression was reduced ... compared with the wild type
Mudunkothge JS, Krizek BA - Three Arabidopsis AIL/PLT genes act in combination to regulate shoot apical meristem function
Visit the PubMed ArticleWe also examined expression of ANT, a marker of lateral organ fate (Elliott et al., 1996; Long and Barton, 2000). Interestingly, ANT mRNA was detected not only in leaf primordia but also in ant-4 ail6-2 ail7-1 meristems
Mudunkothge JS, Krizek BA - Three Arabidopsis AIL/PLT genes act in combination to regulate shoot apical meristem function
Visit the PubMed ArticleUsing in situ hybridization, we find ... AIL6 mRNA is highest in the peripheral regions of the meristem
Mudunkothge JS, Krizek BA - Three Arabidopsis AIL/PLT genes act in combination to regulate shoot apical meristem function
Visit the PubMed Articlein ant-4 ail6-2 ail7-1 7-day-old seedlings ... WUS mRNA levels were reduced
Mudunkothge JS, Krizek BA - Three Arabidopsis AIL/PLT genes act in combination to regulate shoot apical meristem function
Visit the PubMed ArticleWUS mRNA was detected in 13 out of 17 ant-4 ail6-2 ail7-1 meristems with varying intensity levels (Figure 3d–f). In many cases, the WUS expression domain was shifted upward into the L1 and L2 layers of the meristem instead of being confined to the L3 layer as in the wild type
Mudunkothge JS, Krizek BA - Three Arabidopsis AIL/PLT genes act in combination to regulate shoot apical meristem function
Visit the PubMed ArticleUsing in situ hybridization, we find ... AIL7 mRNA is highest in the center of the meristem
Mudunkothge JS, Krizek BA - Three Arabidopsis AIL/PLT genes act in combination to regulate shoot apical meristem function
Visit the PubMed ArticleReal-time RT-PCR revealed that CLV3 mRNA was undetectable in ant-4 ail6-2 ail7-1 7-day-old seedlings
Mudunkothge JS, Krizek BA - Three Arabidopsis AIL/PLT genes act in combination to regulate shoot apical meristem function
Visit the PubMed ArticleExpression of EXP1B was ... down-regulated in the lec1-1 mutant
Junker A, Mönke G, Rutten T, Keilwagen J, Seifert M, Thi TM, Renou JP, Balzergue S, Viehöver P, Hähnel U, Ludwig-Müller J, Altschmied L, Conrad U, Weisshaar B, Bäumlein H - Elongation-related functions of LEAFY COTYLEDON1 during the development of Arabidopsis thaliana
Visit the PubMed Article35S::LEC1::GR seedlings ... Auxin accumulation was restricted to the collet region of the combined DEX/ABA-treated seedlings (Figure 4l), but was not observed in any of the control treatments
Junker A, Mönke G, Rutten T, Keilwagen J, Seifert M, Thi TM, Renou JP, Balzergue S, Viehöver P, Hähnel U, Ludwig-Müller J, Altschmied L, Conrad U, Weisshaar B, Bäumlein H - Elongation-related functions of LEAFY COTYLEDON1 during the development of Arabidopsis thaliana
Visit the PubMed ArticleRD29B ... expression was well correlated with the severity of the LEC1-induced phenotypes
Junker A, Mönke G, Rutten T, Keilwagen J, Seifert M, Thi TM, Renou JP, Balzergue S, Viehöver P, Hähnel U, Ludwig-Müller J, Altschmied L, Conrad U, Weisshaar B, Bäumlein H - Elongation-related functions of LEAFY COTYLEDON1 during the development of Arabidopsis thaliana
Visit the PubMed ArticleExpression of EXP1B was activated in LEC1-induced structures
Junker A, Mönke G, Rutten T, Keilwagen J, Seifert M, Thi TM, Renou JP, Balzergue S, Viehöver P, Hähnel U, Ludwig-Müller J, Altschmied L, Conrad U, Weisshaar B, Bäumlein H - Elongation-related functions of LEAFY COTYLEDON1 during the development of Arabidopsis thaliana
Visit the PubMed ArticleLHCB5 ... was reduced in ... LEC1-induced structures present in seedlings of phenotypes B and C
Junker A, Mönke G, Rutten T, Keilwagen J, Seifert M, Thi TM, Renou JP, Balzergue S, Viehöver P, Hähnel U, Ludwig-Müller J, Altschmied L, Conrad U, Weisshaar B, Bäumlein H - Elongation-related functions of LEAFY COTYLEDON1 during the development of Arabidopsis thaliana
Visit the PubMed Article35S::LEC1::GR ... Repression of DOGT1 ... by LEC1
Junker A, Mönke G, Rutten T, Keilwagen J, Seifert M, Thi TM, Renou JP, Balzergue S, Viehöver P, Hähnel U, Ludwig-Müller J, Altschmied L, Conrad U, Weisshaar B, Bäumlein H - Elongation-related functions of LEAFY COTYLEDON1 during the development of Arabidopsis thaliana
Visit the PubMed Article35S::LEC1::GR ... and activation of DWF4 by LEC1
Junker A, Mönke G, Rutten T, Keilwagen J, Seifert M, Thi TM, Renou JP, Balzergue S, Viehöver P, Hähnel U, Ludwig-Müller J, Altschmied L, Conrad U, Weisshaar B, Bäumlein H - Elongation-related functions of LEAFY COTYLEDON1 during the development of Arabidopsis thaliana
Visit the PubMed ArticleLHCA1 was reduced in ... LEC1-induced structures present in seedlings of phenotypes B and C
Junker A, Mönke G, Rutten T, Keilwagen J, Seifert M, Thi TM, Renou JP, Balzergue S, Viehöver P, Hähnel U, Ludwig-Müller J, Altschmied L, Conrad U, Weisshaar B, Bäumlein H - Elongation-related functions of LEAFY COTYLEDON1 during the development of Arabidopsis thaliana
Visit the PubMed ArticleLHCA1 was ... highly expressed in the lec1 mutant
Junker A, Mönke G, Rutten T, Keilwagen J, Seifert M, Thi TM, Renou JP, Balzergue S, Viehöver P, Hähnel U, Ludwig-Müller J, Altschmied L, Conrad U, Weisshaar B, Bäumlein H - Elongation-related functions of LEAFY COTYLEDON1 during the development of Arabidopsis thaliana
Visit the PubMed ArticleLEC1-LIKE (L1L) ... a NF-YB6 subunit-encoding gene, was highly expressed in the LEC1-induced structures in seedlings of phenotypes B and C
Junker A, Mönke G, Rutten T, Keilwagen J, Seifert M, Thi TM, Renou JP, Balzergue S, Viehöver P, Hähnel U, Ludwig-Müller J, Altschmied L, Conrad U, Weisshaar B, Bäumlein H - Elongation-related functions of LEAFY COTYLEDON1 during the development of Arabidopsis thaliana
Visit the PubMed ArticleThe LEC1-induced embryonic structures resembled those resulting from ectopic expression of LEC2, which have been associated with enhanced auxin biosynthesis (Stone et al., 2008). Auxin-responsive DR5 activity in phenotype A, B and C seedlings was assessed using a GFP reporter (Ulmasov et al., 1997). Localized high activity of DR5 was detected in the lowermost epidermal cell layers of the hypocotyl (the elongation zone), particularly in the more severely affected phenotype A seedlings (Figure 3a,b). In the less severely affected phenotype B seedlings, a ring of GFP-expressing cells was only visible at the junction between the hypocotyl and the primary root of 7-day-old seedlings (Figure 3c,d), which became the site of callus formation and eventual somatic embryogenesis (Figure 3e–i). After appearance of the callus, the DR5 activity in the collet was no longer visible (Figure 3f). In phenotype C seedlings, GFP expression was detected in the hypocotyl, but was not confined to the elongation zone
Junker A, Mönke G, Rutten T, Keilwagen J, Seifert M, Thi TM, Renou JP, Balzergue S, Viehöver P, Hähnel U, Ludwig-Müller J, Altschmied L, Conrad U, Weisshaar B, Bäumlein H - Elongation-related functions of LEAFY COTYLEDON1 during the development of Arabidopsis thaliana
Visit the PubMed ArticleThe seed transcription factor genes ABI3 ... highly expressed after induction of LEC1 compared to the uninduced control plant
Junker A, Mönke G, Rutten T, Keilwagen J, Seifert M, Thi TM, Renou JP, Balzergue S, Viehöver P, Hähnel U, Ludwig-Müller J, Altschmied L, Conrad U, Weisshaar B, Bäumlein H - Elongation-related functions of LEAFY COTYLEDON1 during the development of Arabidopsis thaliana
Visit the PubMed ArticleCAB4 ... was ... highly expressed in the lec1 mutant
Junker A, Mönke G, Rutten T, Keilwagen J, Seifert M, Thi TM, Renou JP, Balzergue S, Viehöver P, Hähnel U, Ludwig-Müller J, Altschmied L, Conrad U, Weisshaar B, Bäumlein H - Elongation-related functions of LEAFY COTYLEDON1 during the development of Arabidopsis thaliana
Visit the PubMed ArticleLHCB5 ... was ... highly expressed in the lec1 mutant
Junker A, Mönke G, Rutten T, Keilwagen J, Seifert M, Thi TM, Renou JP, Balzergue S, Viehöver P, Hähnel U, Ludwig-Müller J, Altschmied L, Conrad U, Weisshaar B, Bäumlein H - Elongation-related functions of LEAFY COTYLEDON1 during the development of Arabidopsis thaliana
Visit the PubMed ArticleThe seed transcription factor genes ... FUS3 ... highly expressed after induction of LEC1 compared to the uninduced control plant
Junker A, Mönke G, Rutten T, Keilwagen J, Seifert M, Thi TM, Renou JP, Balzergue S, Viehöver P, Hähnel U, Ludwig-Müller J, Altschmied L, Conrad U, Weisshaar B, Bäumlein H - Elongation-related functions of LEAFY COTYLEDON1 during the development of Arabidopsis thaliana
Visit the PubMed Article35S::LEC1::GR seedlings ... Expression of YUC10 was induced by DEX/ABA treatment (Figure 5a). YUC10 was also highly expressed in the LEC1-induced structures, and its level was correlated with the level of embryonic features (relative to phenotype C, the phenotypes A and B do have more embryonic features) of the various phenotypes (high in phenotypes A and B, low in phenotype C; Figure 5b).
Junker A, Mönke G, Rutten T, Keilwagen J, Seifert M, Thi TM, Renou JP, Balzergue S, Viehöver P, Hähnel U, Ludwig-Müller J, Altschmied L, Conrad U, Weisshaar B, Bäumlein H - Elongation-related functions of LEAFY COTYLEDON1 during the development of Arabidopsis thaliana
Visit the PubMed ArticleXTH9, encoding a xyloglucan hydrolase ... was down-regulated in ... LEC1-induced phenotype B and C seedlings
Junker A, Mönke G, Rutten T, Keilwagen J, Seifert M, Thi TM, Renou JP, Balzergue S, Viehöver P, Hähnel U, Ludwig-Müller J, Altschmied L, Conrad U, Weisshaar B, Bäumlein H - Elongation-related functions of LEAFY COTYLEDON1 during the development of Arabidopsis thaliana
Visit the PubMed ArticleThe seed transcription factor genes ... LEC2 ... highly expressed after induction of LEC1 compared to the uninduced control plant
Junker A, Mönke G, Rutten T, Keilwagen J, Seifert M, Thi TM, Renou JP, Balzergue S, Viehöver P, Hähnel U, Ludwig-Müller J, Altschmied L, Conrad U, Weisshaar B, Bäumlein H - Elongation-related functions of LEAFY COTYLEDON1 during the development of Arabidopsis thaliana
Visit the PubMed ArticleExpression of IAA19 ... was elevated in the LEC1-induced structures
Junker A, Mönke G, Rutten T, Keilwagen J, Seifert M, Thi TM, Renou JP, Balzergue S, Viehöver P, Hähnel U, Ludwig-Müller J, Altschmied L, Conrad U, Weisshaar B, Bäumlein H - Elongation-related functions of LEAFY COTYLEDON1 during the development of Arabidopsis thaliana
Visit the PubMed ArticleExpression of ... IAA5 was elevated in the LEC1-induced structures
Junker A, Mönke G, Rutten T, Keilwagen J, Seifert M, Thi TM, Renou JP, Balzergue S, Viehöver P, Hähnel U, Ludwig-Müller J, Altschmied L, Conrad U, Weisshaar B, Bäumlein H - Elongation-related functions of LEAFY COTYLEDON1 during the development of Arabidopsis thaliana
Visit the PubMed ArticleCAB4 ... was reduced in ... LEC1-induced structures present in seedlings of phenotypes B and C
Junker A, Mönke G, Rutten T, Keilwagen J, Seifert M, Thi TM, Renou JP, Balzergue S, Viehöver P, Hähnel U, Ludwig-Müller J, Altschmied L, Conrad U, Weisshaar B, Bäumlein H - Elongation-related functions of LEAFY COTYLEDON1 during the development of Arabidopsis thaliana
Visit the PubMed ArticleLEC1-LIKE (L1L ... strongly repressed expression in the lec1 mutant embryo
Junker A, Mönke G, Rutten T, Keilwagen J, Seifert M, Thi TM, Renou JP, Balzergue S, Viehöver P, Hähnel U, Ludwig-Müller J, Altschmied L, Conrad U, Weisshaar B, Bäumlein H - Elongation-related functions of LEAFY COTYLEDON1 during the development of Arabidopsis thaliana
Visit the PubMed ArticleRAB18 expression was well correlated with the severity of the LEC1-induced phenotypes
Junker A, Mönke G, Rutten T, Keilwagen J, Seifert M, Thi TM, Renou JP, Balzergue S, Viehöver P, Hähnel U, Ludwig-Müller J, Altschmied L, Conrad U, Weisshaar B, Bäumlein H - Elongation-related functions of LEAFY COTYLEDON1 during the development of Arabidopsis thaliana
Visit the PubMed ArticleSTU:GUS ... At 3 days after germination, GUS activity was detected in shoot apices and roots (Fig. 2B). Intense staining patterns were observed in the same regions of 1-week-old and 2-week-old seedlings with gradually increased staining in the leaf vasculature
Lee LY, Hou X, Fang L, Fan S, Kumar PP, Yu H - STUNTED mediates the control of cell proliferation by GA in Arabidopsis
Visit the PubMed ArticleSMR1 ... downregulated in 35S:STU
Lee LY, Hou X, Fang L, Fan S, Kumar PP, Yu H - STUNTED mediates the control of cell proliferation by GA in Arabidopsis
Visit the PubMed ArticleSTU ... transcript levels were significantly upregulated by GA in ga1-3
Lee LY, Hou X, Fang L, Fan S, Kumar PP, Yu H - STUNTED mediates the control of cell proliferation by GA in Arabidopsis
Visit the PubMed ArticleSTU:GUS ... GUS staining was strong in the center of the inflorescence apex and the anthers of floral buds at flower stages 9 to 12
Lee LY, Hou X, Fang L, Fan S, Kumar PP, Yu H - STUNTED mediates the control of cell proliferation by GA in Arabidopsis
Visit the PubMed Articlega1-3 background ... RGA had the strongest effect on STU ... expression
Lee LY, Hou X, Fang L, Fan S, Kumar PP, Yu H - STUNTED mediates the control of cell proliferation by GA in Arabidopsis
Visit the PubMed ArticleGFP-STU signals were observed throughout the cell
Lee LY, Hou X, Fang L, Fan S, Kumar PP, Yu H - STUNTED mediates the control of cell proliferation by GA in Arabidopsis
Visit the PubMed ArticleSMR1 ... upregulated in stu-1
Lee LY, Hou X, Fang L, Fan S, Kumar PP, Yu H - STUNTED mediates the control of cell proliferation by GA in Arabidopsis
Visit the PubMed ArticleGA treatment ... 35S:STU ... inhibiting the expression of SMR1
Lee LY, Hou X, Fang L, Fan S, Kumar PP, Yu H - STUNTED mediates the control of cell proliferation by GA in Arabidopsis
Visit the PubMed Articlein shoot apices ... PAC inhibited STU:GUS activity
Lee LY, Hou X, Fang L, Fan S, Kumar PP, Yu H - STUNTED mediates the control of cell proliferation by GA in Arabidopsis
Visit the PubMed Articlega1-3 background ... on STU ... GAI had a redundant effect
Lee LY, Hou X, Fang L, Fan S, Kumar PP, Yu H - STUNTED mediates the control of cell proliferation by GA in Arabidopsis
Visit the PubMed ArticleGA treatment promoted STU:GUS activity in shoot apices when compared with mock treatment
Lee LY, Hou X, Fang L, Fan S, Kumar PP, Yu H - STUNTED mediates the control of cell proliferation by GA in Arabidopsis
Visit the PubMed ArticleAt 8 days after germination, transcript levels of ... KNOLLE in stu-1 were reduced by approximately ... one-half ... when compared with wild ... type plants
Lee LY, Hou X, Fang L, Fan S, Kumar PP, Yu H - STUNTED mediates the control of cell proliferation by GA in Arabidopsis
Visit the PubMed ArticleIn the absence of RGA in ga1-3 rga-t2, STU was significantly upregulated
Lee LY, Hou X, Fang L, Fan S, Kumar PP, Yu H - STUNTED mediates the control of cell proliferation by GA in Arabidopsis
Visit the PubMed ArticleSTU expression was downregulated from 4 hours after RGA induction in dexamethasone-treated seedlings
Lee LY, Hou X, Fang L, Fan S, Kumar PP, Yu H - STUNTED mediates the control of cell proliferation by GA in Arabidopsis
Visit the PubMed Articlega1-3 background ... RGL1 ... slightly upregulated STU
Lee LY, Hou X, Fang L, Fan S, Kumar PP, Yu H - STUNTED mediates the control of cell proliferation by GA in Arabidopsis
Visit the PubMed ArticleSTU:GUS ... A close examination of longitudinal sections of a stage 10 flower revealed the ... specific staining in the tapetum
Lee LY, Hou X, Fang L, Fan S, Kumar PP, Yu H - STUNTED mediates the control of cell proliferation by GA in Arabidopsis
Visit the PubMed ArticleSTU:GUS ... At stage 14, GUS staining was mainly detected in ... pollen
Lee LY, Hou X, Fang L, Fan S, Kumar PP, Yu H - STUNTED mediates the control of cell proliferation by GA in Arabidopsis
Visit the PubMed ArticleThere was no change in STU transcript levels between wild-type and rga-t2 seedlings
Lee LY, Hou X, Fang L, Fan S, Kumar PP, Yu H - STUNTED mediates the control of cell proliferation by GA in Arabidopsis
Visit the PubMed ArticleSIM ... upregulated in stu-1
Lee LY, Hou X, Fang L, Fan S, Kumar PP, Yu H - STUNTED mediates the control of cell proliferation by GA in Arabidopsis
Visit the PubMed ArticleSIM ... downregulated in 35S:STU
Lee LY, Hou X, Fang L, Fan S, Kumar PP, Yu H - STUNTED mediates the control of cell proliferation by GA in Arabidopsis
Visit the PubMed ArticleRGA induction ... seedlings ... under a combined treatment of dexamethasone and cycloheximide ... STU exhibited a similar decreased expression pattern
Lee LY, Hou X, Fang L, Fan S, Kumar PP, Yu H - STUNTED mediates the control of cell proliferation by GA in Arabidopsis
Visit the PubMed ArticleSTU:GUS ... GUS staining remained in main inflorescence apices and was also strongly detected in secondary inflorescence meristems that were subtended by cauline leaves
Lee LY, Hou X, Fang L, Fan S, Kumar PP, Yu H - STUNTED mediates the control of cell proliferation by GA in Arabidopsis
Visit the PubMed Articlega1-3 background ... RGL2 slightly upregulated STU
Lee LY, Hou X, Fang L, Fan S, Kumar PP, Yu H - STUNTED mediates the control of cell proliferation by GA in Arabidopsis
Visit the PubMed ArticleGA treatment ... 35S:STU ... inhibiting the expression of ... SIM
Lee LY, Hou X, Fang L, Fan S, Kumar PP, Yu H - STUNTED mediates the control of cell proliferation by GA in Arabidopsis
Visit the PubMed ArticleThe CYCLIN B1 (CYCB1;1)-GUS reporter ... was evidently reduced in root meristems of stu-1 when compared with wild-type plants under mock treatment
Lee LY, Hou X, Fang L, Fan S, Kumar PP, Yu H - STUNTED mediates the control of cell proliferation by GA in Arabidopsis
Visit the PubMed ArticleSTU:GUS ... At stage 14, GUS staining was mainly detected in ovules
Lee LY, Hou X, Fang L, Fan S, Kumar PP, Yu H - STUNTED mediates the control of cell proliferation by GA in Arabidopsis
Visit the PubMed Articlewe tested the effect of picloram on gene expression and compared it with the effect of shade ... IAA29 expression was induced by both treatments in pif4pif5 and the wild type ... However, the expression of IAA29 in pif4pif5 never reached wild type levels when seedlings were treated by picloram or shade
Hornitschek P, Kohnen MV, Lorrain S, Rougemont J, Ljung K, López-Vidriero I, Franco-Zorrilla JM, Solano R, Trevisan M, Pradervand S, Xenarios I, Fankhauser C - Phytochrome interacting factors 4 and 5 control seedling growth in changing light conditions by directly controlling auxin signaling
Visit the PubMed Articlereduced induction by low R/FR in pif4pif5. This small group contains ... CKX5
Hornitschek P, Kohnen MV, Lorrain S, Rougemont J, Ljung K, López-Vidriero I, Franco-Zorrilla JM, Solano R, Trevisan M, Pradervand S, Xenarios I, Fankhauser C - Phytochrome interacting factors 4 and 5 control seedling growth in changing light conditions by directly controlling auxin signaling
Visit the PubMed ArticleHFR1 was upregulated by shade but not picloram ... in pif4pif5 and the wild type
Hornitschek P, Kohnen MV, Lorrain S, Rougemont J, Ljung K, López-Vidriero I, Franco-Zorrilla JM, Solano R, Trevisan M, Pradervand S, Xenarios I, Fankhauser C - Phytochrome interacting factors 4 and 5 control seedling growth in changing light conditions by directly controlling auxin signaling
Visit the PubMed Articlereduced induction by low R/FR in pif4pif5. This small group contains genes identified previously as dependent on PIF4 and PIF5 for regulation, such as HFR1
Hornitschek P, Kohnen MV, Lorrain S, Rougemont J, Ljung K, López-Vidriero I, Franco-Zorrilla JM, Solano R, Trevisan M, Pradervand S, Xenarios I, Fankhauser C - Phytochrome interacting factors 4 and 5 control seedling growth in changing light conditions by directly controlling auxin signaling
Visit the PubMed ArticleIAA29 ... showed a slightly reduced expression in pif4pif5 exposed to low R/FR but had strongly reduced expression in high R/FR
Hornitschek P, Kohnen MV, Lorrain S, Rougemont J, Ljung K, López-Vidriero I, Franco-Zorrilla JM, Solano R, Trevisan M, Pradervand S, Xenarios I, Fankhauser C - Phytochrome interacting factors 4 and 5 control seedling growth in changing light conditions by directly controlling auxin signaling
Visit the PubMed Articlereduced induction by low R/FR in pif4pif5. This small group contains ... PIL2
Hornitschek P, Kohnen MV, Lorrain S, Rougemont J, Ljung K, López-Vidriero I, Franco-Zorrilla JM, Solano R, Trevisan M, Pradervand S, Xenarios I, Fankhauser C - Phytochrome interacting factors 4 and 5 control seedling growth in changing light conditions by directly controlling auxin signaling
Visit the PubMed Articlereduced induction by low R/FR in pif4pif5. This small group contains ... FHL
Hornitschek P, Kohnen MV, Lorrain S, Rougemont J, Ljung K, López-Vidriero I, Franco-Zorrilla JM, Solano R, Trevisan M, Pradervand S, Xenarios I, Fankhauser C - Phytochrome interacting factors 4 and 5 control seedling growth in changing light conditions by directly controlling auxin signaling
Visit the PubMed Articlereduced induction by low R/FR in pif4pif5. This small group contains genes identified previously as dependent on PIF4 and PIF5 for regulation, such as ... ATHB2
Hornitschek P, Kohnen MV, Lorrain S, Rougemont J, Ljung K, López-Vidriero I, Franco-Zorrilla JM, Solano R, Trevisan M, Pradervand S, Xenarios I, Fankhauser C - Phytochrome interacting factors 4 and 5 control seedling growth in changing light conditions by directly controlling auxin signaling
Visit the PubMed ArticleYUC8 ... showed a slightly reduced expression in pif4pif5 exposed to low R/FR but had strongly reduced expression in high R/FR
Hornitschek P, Kohnen MV, Lorrain S, Rougemont J, Ljung K, López-Vidriero I, Franco-Zorrilla JM, Solano R, Trevisan M, Pradervand S, Xenarios I, Fankhauser C - Phytochrome interacting factors 4 and 5 control seedling growth in changing light conditions by directly controlling auxin signaling
Visit the PubMed ArticleVLN2 is quite suitable to investigate the functional relationship between villins and plant development because it is widely expressed throughout vegetative tissues
Bao C, Wang J, Zhang R, Zhang B, Zhang H, Zhou Y, Huang S - Arabidopsis VILLIN2 and VILLIN3 act redundantly in sclerenchyma development via bundling of actin filaments
Visit the PubMed ArticleQuantitative PCR analysis showed that loss of function of both VLN2 and VLN3 did not affect expression of ... the lignin biosynthetic ... 4CL1 (for hydroxycinnamate CoA ligase)
Bao C, Wang J, Zhang R, Zhang B, Zhang H, Zhou Y, Huang S - Arabidopsis VILLIN2 and VILLIN3 act redundantly in sclerenchyma development via bundling of actin filaments
Visit the PubMed ArticleQuantitative PCR analysis showed that loss of function of both VLN2 and VLN3 did not affect expression of the cellulose synthase ... CesA8
Bao C, Wang J, Zhang R, Zhang B, Zhang H, Zhou Y, Huang S - Arabidopsis VILLIN2 and VILLIN3 act redundantly in sclerenchyma development via bundling of actin filaments
Visit the PubMed ArticleQuantitative PCR analysis showed that loss of function of both VLN2 and VLN3 did not affect expression of ... the lignin biosynthetic ... CCoAOMT (for caffeoyl CoA O-methyltransferase)
Bao C, Wang J, Zhang R, Zhang B, Zhang H, Zhou Y, Huang S - Arabidopsis VILLIN2 and VILLIN3 act redundantly in sclerenchyma development via bundling of actin filaments
Visit the PubMed ArticleQuantitative PCR analysis showed that loss of function of both VLN2 and VLN3 did not affect expression of the cellulose synthase ... CesA7
Bao C, Wang J, Zhang R, Zhang B, Zhang H, Zhou Y, Huang S - Arabidopsis VILLIN2 and VILLIN3 act redundantly in sclerenchyma development via bundling of actin filaments
Visit the PubMed Articlewe found that the VLN2 and VLN3 expression patterns overlapped considerably (Figure S1A); this was confirmed by RT-PCR analysis of several tissues, including stems, pedicels, roots and hypocotyls
Bao C, Wang J, Zhang R, Zhang B, Zhang H, Zhou Y, Huang S - Arabidopsis VILLIN2 and VILLIN3 act redundantly in sclerenchyma development via bundling of actin filaments
Visit the PubMed Articlewe found that the VLN2 and VLN3 expression patterns overlapped considerably (Figure S1A); this was confirmed by RT-PCR analysis of several tissues, including stems, pedicels, roots and hypocotyls
Bao C, Wang J, Zhang R, Zhang B, Zhang H, Zhou Y, Huang S - Arabidopsis VILLIN2 and VILLIN3 act redundantly in sclerenchyma development via bundling of actin filaments
Visit the PubMed Articlea specific signal ... was detected ... in the adaxial domain of developing leaves ... for ... ATHB4
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed ArticleREV-β-glucuronidase (GUS) reporter (rev-9 ... expressed in ... the shoot apical meristem region of young seedlings
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed ArticleOur analysis revealed a strong transcriptional upregulation of ... HAT3 ... in response to DEX induction in 35S::GR-REVd transgenic plants ... To verify that the mode of regulation of these HD-ZIPII genes is of a direct nature, we performed the experiment in the presence of the protein biosynthesis inhibitor cycloheximide (CHX). Also in the presence of CHX ... HAT3 ... showed significant expression changes in response to DEX application, which implies that they are direct REV-target genes
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed ArticleATHB2/HAT4 ... expression ... is significantly lower in rev-5
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed Articlea TAA1 promoter–GUS reporter line ... expressed in ... the vasculature
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed ArticleOur analysis revealed a strong transcriptional upregulation of ... ATHB2/HAT4 ... in response to DEX induction in 35S::GR-REVd transgenic plants ... To verify that the mode of regulation of these HD-ZIPII genes is of a direct nature, we performed the experiment in the presence of the protein biosynthesis inhibitor cycloheximide (CHX). Also in the presence of CHX ... ATHB2/HAT4 ... showed significant expression changes in response to DEX application, which implies that they are direct REV-target genes
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed ArticleREV-β-glucuronidase (GUS) reporter (rev-9) ... expressed in ... the vasculature
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed Articlein response to DEX induction in 35S::GR-REVd transgenic plants ... the expression of HAT1 ... did not change significantly
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed ArticleTAA1 ... expression levels are slightly lower in rev-5
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed ArticleHAT3 ... is markedly induced in wild-type plants in response to shade treatment
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed ArticleOur analysis revealed a strong transcriptional upregulation of ... ATHB4 ... in response to DEX induction in 35S::GR-REVd transgenic plants ... To verify that the mode of regulation of these HD-ZIPII genes is of a direct nature, we performed the experiment in the presence of the protein biosynthesis inhibitor cycloheximide (CHX). Also in the presence of CHX ... ATHB4 showed significant expression changes in response to DEX application, which implies that they are direct REV-target genes
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed ArticleIn the 35S::miR165a transgenic line, expression of ... YUC5 is lower compared with wild-type
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed Articleexpression of ... TAA1 ... is reduced significantly by DEX induction of 35S::FLAG-GR-KAN1 plants
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed Articlea specific signal ... was detected ... in the adaxial domain of developing leaves ... for ... TAA1
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed Articlea specific signal for ... was detected in the vasculature ... for ... ATHB4
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed ArticleThe expression of TAA1 does not change in response to shade
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed ArticleATHB2/HAT4 ... is markedly induced in wild-type plants in response to shade treatment
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed Articlein response to DEX induction in 35S::GR-REVd transgenic plants ... Even though identified as putative targets, the expression of ... HAT22 did not change significantly
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed ArticleTo find out if REV can transcriptionally regulate other genes that encode auxin biosynthetic enzymes, we analyzed systematically expression changes of all YUCCA-type and TAA1-related genes in wild-type Col-0 and transgenic 35S::GR-REVd plants elicited by DEX induction (Figure S1). This study revealed that YUCCA5 expression is also regulated by REV ... To confirm that REV can upregulate ... YUC5 expression, we performed quantitative RT-PCR reaction on wild-type Col-0 and transgenic 35S::GR-REVd seedlings with and without DEX application. These experiments demonstrated that the level of transcription is increased in ... YUC5 in response to DEX application in the transgenic 35S::GR-REVd plants ... The induction also occurs in seedlings pre-treated with cycloheximide (CHX), which supports a direct role for REV in the regulation of these genes
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed ArticleAs shown for the published 35S::KAN1-GR plants, our 35S::FLAG-GR-KAN1 line was also able to strongly repress expression of the ASYMMETRIC LEAVES2 gene
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed Articlewe performed in situ hybridizations on transverse shoot apical meristem sections. As expected, a specific signal for REV was detected in the vasculature
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed Articleexpression of ... YUC5 is reduced significantly by DEX induction of 35S::FLAG-GR-KAN1 plants
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed ArticleATHB4 ... expression ... is significantly lower in ... 35S::ZPR3 plants
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed ArticleATHB4 ... is markedly induced in wild-type plants in response to shade treatment
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed Articlea TAA1 promoter–GUS reporter line ... expressed in ... the shoot apical meristem region of young seedlings
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed Articlea specific signal ... was detected ... in the adaxial domain of developing leaves ... for ... HAT3
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed Articlein response to DEX induction in 35S::GR-REVd transgenic plants ... Even though identified as putative targets, the expression of ... HAT14 ... did not change significantly
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed Articlea specific signal ... was detected in the vasculature ... for ... TAA1
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed ArticleHAT3 ... expression ... is significantly lower in rev-5
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed Articleexpression of HAT2 ... is reduced significantly by DEX induction of 35S::FLAG-GR-KAN1 plants
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed ArticleIn the 35S::miR165a transgenic line, expression of ... ATHB4 ... is lower compared with wild-type
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed ArticleTo confirm that REV can upregulate ... TAA1 ... expression, we performed quantitative RT-PCR reaction on wild-type Col-0 and transgenic 35S::GR-REVd seedlings with and without DEX application. These experiments demonstrated that the level of transcription is increased in ... TAA1 ... in response to DEX application in the transgenic 35S::GR-REVd plants ... The induction also occurs in seedlings pre-treated with cycloheximide (CHX), which supports a direct role for REV in the regulation of these genes
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed ArticleHAT2 ... is markedly induced in wild-type plants in response to shade treatment
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed Articlewe performed in situ hybridizations on transverse shoot apical meristem sections. As expected, a specific signal for REV was detected ... in the adaxial domain of developing leaves
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed ArticleHAT3 ... expression ... is significantly lower in ... 35S::ZPR3 plants
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed ArticleYUC5 ... expression ... is significantly lower in ... 35S::ZPR3 plants
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed ArticleHAT2 ... expression ... is significantly lower in ... 35S::ZPR3 plants
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed ArticleHAT2 ... expression ... is significantly lower in rev-5
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed Articlea specific signal ... was detected ... in the adaxial domain of developing leaves ... for HAT2
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed Articlein response to simulated canopy shade ... expression of PHABULOSA ... remain unchanged
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed ArticleTo determine whether REV is able to induce auxin levels, we have introduced the 35S::GR-REVd transgene into plants that harbor the DR5::GUS reporter by crossing. Col-0 wild-type plants showed DR5-GUS expression in the leaf margins and hydathodes (Figure 6a). Induction of GR-REVd by DEX application results in a strong GUS signal throughout the leaf lamina
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed Articlea specific signal ... was detected in the vasculature ... for ... HAT3
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed ArticleOur analysis revealed a strong transcriptional upregulation of HAT2 ... in response to DEX induction in 35S::GR-REVd transgenic plants ... To verify that the mode of regulation of these HD-ZIPII genes is of a direct nature, we performed the experiment in the presence of the protein biosynthesis inhibitor cycloheximide (CHX). Also in the presence of CHX, HAT2 ... showed significant expression changes in response to DEX application, which implies that they are direct REV-target genes
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed ArticleIn the 35S::miR165a transgenic line ... the expression of HAT3 is slightly higher than in the wild-type controls
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed Articlein response to simulated canopy shade ... expression of ... PHAVOLUTA remain unchanged
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed ArticleATHB2/HAT4 ... expression ... is significantly lower in ... 35S::ZPR3 plants
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed ArticleYUC5 ... expression ... is significantly lower in rev-5
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed ArticleATHB4 ... expression ... is significantly lower in rev-5
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed ArticleIn the 35S::miR165a transgenic line, expression of ATHB2/HAT4 ... is lower compared with wild-type
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed ArticleTAA1 ... expression levels are slightly lower in ... 35S::ZPR3 plants compared with wild-type
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed Articlea specific signal ... was detected in the vasculature ... for HAT2
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed ArticleYUC5 is markedly induced in wild-type plants in response to shade treatment
Brandt R, Salla-Martret M, Bou-Torrent J, Musielak T, Stahl M, Lanz C, Ott F, Schmid M, Greb T, Schwarz M, Choi SB, Barton MK, Reinhart BJ, Liu T, Quint M, Palauqui JC, Martínez-García JF, Wenkel S - Genome-wide binding-site analysis of REVOLUTA reveals a link between leaf patterning and light-mediated growth responses
Visit the PubMed Articlepetioles a ... transcript levels of BOP2 ... reduced in ... dcl1-9
Jover-Gil S, Candela H, Robles P, Aguilera V, Barrero JM, Micol JL, Ponce MR - The microRNA pathway genes AGO1, HEN1 and HYL1 participate in leaf proximal-distal, venation and stomatal patterning in Arabidopsis
Visit the PubMed Articlepetioles ... transcript levels of BOP2 ... reduced in ... ago1-52
Jover-Gil S, Candela H, Robles P, Aguilera V, Barrero JM, Micol JL, Ponce MR - The microRNA pathway genes AGO1, HEN1 and HYL1 participate in leaf proximal-distal, venation and stomatal patterning in Arabidopsis
Visit the PubMed Articlelaminae ... transcript levels of BOP2 ... reduced in ... dcl1-9
Jover-Gil S, Candela H, Robles P, Aguilera V, Barrero JM, Micol JL, Ponce MR - The microRNA pathway genes AGO1, HEN1 and HYL1 participate in leaf proximal-distal, venation and stomatal patterning in Arabidopsis
Visit the PubMed Articlelaminae ... transcript levels of BOP2 ... reduced in ... ago1-52
Jover-Gil S, Candela H, Robles P, Aguilera V, Barrero JM, Micol JL, Ponce MR - The microRNA pathway genes AGO1, HEN1 and HYL1 participate in leaf proximal-distal, venation and stomatal patterning in Arabidopsis
Visit the PubMed Articlequantitative RT-PCR ... increased AGL16 transcript levels in the petioles ... dcl1-9
Jover-Gil S, Candela H, Robles P, Aguilera V, Barrero JM, Micol JL, Ponce MR - The microRNA pathway genes AGO1, HEN1 and HYL1 participate in leaf proximal-distal, venation and stomatal patterning in Arabidopsis
Visit the PubMed Articlequantitative RT-PCR ... increased AGL16 transcript ... levels in ... laminae of ago1-52
Jover-Gil S, Candela H, Robles P, Aguilera V, Barrero JM, Micol JL, Ponce MR - The microRNA pathway genes AGO1, HEN1 and HYL1 participate in leaf proximal-distal, venation and stomatal patterning in Arabidopsis
Visit the PubMed Articlequantitative RT-PCR ... increased AGL16 transcript levels in the petioles ... of ago1-52
Jover-Gil S, Candela H, Robles P, Aguilera V, Barrero JM, Micol JL, Ponce MR - The microRNA pathway genes AGO1, HEN1 and HYL1 participate in leaf proximal-distal, venation and stomatal patterning in Arabidopsis
Visit the PubMed Articlequantitative RT-PCR ... increased AGL16 transcript levels in ... laminae of ... dcl1-9
Jover-Gil S, Candela H, Robles P, Aguilera V, Barrero JM, Micol JL, Ponce MR - The microRNA pathway genes AGO1, HEN1 and HYL1 participate in leaf proximal-distal, venation and stomatal patterning in Arabidopsis
Visit the PubMed ArticleIn order to identify host factors essential for viral infection, seeds of Col-16 were EMS-mutagenized and M2 individuals were phenotyped for reduction or loss of MP17:GFP fluorescence ... The its1/MP17:GFP suppressor mutant is characterized by loss of MP17:GFP fluorescence and protein in source leaves (Figure 1a,b). Further analysis showed that MP17:GFP fluorescence is not absent per se. MP17:GFP fluorescence remains in cotyledons (Figure S1), and silencing increases during source leaf development, reminiscent of co-suppression
Thran M, Link K, Sonnewald U - The Arabidopsis DCP2 gene is required for proper mRNA turnover and prevents transgene silencing in Arabidopsis
Visit the PubMed ArticleTo exclude MP17:GFP-specific effects, we tested whether independent transgenes are silenced in the its1 background. To this end, enhanced soluble GFP (eGFP) was introgressed and the MP17:GFP transgene was replaced by segregation. We observed a loss of eGFP fluorescence in almost all aerial organs of the its1/eGFP F2 individuals (Figure 2a,b), accompanied by a loss of eGFP mRNA and accumulation of eGFP siRNAs (Figure 2c,d). Further examination showed that eGFP silencing is rapidly initiated and spreads to all aerial parts of the its1/eGFP mutant (Figure 2a). The nexus of silencing varied in time and location, which indicates that a PTGS mechanism caused the silencing in the its1 mutant. To investigate this, we infected the its1/eGFP mutant with turnip mosaic virus (TuMV), which encodes the HcPro silencing suppressor. All TuMV-infected its1/eGFP mutants displayed reversion of silencing accompanied by a resumption of eGFP fluorescence, an increase in eGFP mRNA and a reduction of eGFP siRNA abundance (Figure 2b–d). Taken together, these results suggest that transgenes can become the target of PTGS in the its1 mutant
Thran M, Link K, Sonnewald U - The Arabidopsis DCP2 gene is required for proper mRNA turnover and prevents transgene silencing in Arabidopsis
Visit the PubMed ArticleAt later stages, when serrations grew out in wild type, a diffuse expression of DR5::GFP was visible throughout the leaf margin of 35S::DPA4-9 plants
Engelhorn J, Reimer JJ, Leuz I, Göbel U, Huettel B, Farrona S, Turck F - Development-related PcG target in the apex 4 controls leaf margin architecture in Arabidopsis thaliana
Visit the PubMed ArticleDPA4 expression was observed in the leaf sinus
Engelhorn J, Reimer JJ, Leuz I, Göbel U, Huettel B, Farrona S, Turck F - Development-related PcG target in the apex 4 controls leaf margin architecture in Arabidopsis thaliana
Visit the PubMed ArticleIn strong DPA4-overexpressing plants, no CUC2:VENUS signal was detected
Engelhorn J, Reimer JJ, Leuz I, Göbel U, Huettel B, Farrona S, Turck F - Development-related PcG target in the apex 4 controls leaf margin architecture in Arabidopsis thaliana
Visit the PubMed Articlein situ hybridisation revealed that expression of DPA4 was still restricted to discrete clusters of few cells in clf/swn callus tissue, indicating that additional regulation, apart from PcG-mediated repression, is involved in the tight regulation of DPA4
Engelhorn J, Reimer JJ, Leuz I, Göbel U, Huettel B, Farrona S, Turck F - Development-related PcG target in the apex 4 controls leaf margin architecture in Arabidopsis thaliana
Visit the PubMed ArticleExpression analysis in the single ... mutants of MIR164A ... confirmed that ... CUC2 expression was increased
Engelhorn J, Reimer JJ, Leuz I, Göbel U, Huettel B, Farrona S, Turck F - Development-related PcG target in the apex 4 controls leaf margin architecture in Arabidopsis thaliana
Visit the PubMed ArticleTo test whether DPA4 affects auxin distribution, we analysed expression of the DR5::GFP auxin reporter in 35S::DPA4 plants (Benková et al., 2003). 35S::DPA4-9 plants showed GFP signal at the tip of initiating leaves, comparable to wild type, but failed to establish discrete auxin maxima at other positions, even at growth stages when wild-type leaves had already initiated serrations
Engelhorn J, Reimer JJ, Leuz I, Göbel U, Huettel B, Farrona S, Turck F - Development-related PcG target in the apex 4 controls leaf margin architecture in Arabidopsis thaliana
Visit the PubMed ArticleIn situ hybridisation experiments showed that expression of DPA4 within the shoot apex is tightly restricted to the areas of primordia formation. In vegetative and transition apices, expression was observed at the emerging boundary between the shoot apex and the leaf primordium. DPA4 expression persisted in this area as the leaf developed
Engelhorn J, Reimer JJ, Leuz I, Göbel U, Huettel B, Farrona S, Turck F - Development-related PcG target in the apex 4 controls leaf margin architecture in Arabidopsis thaliana
Visit the PubMed ArticleIn the single clf mutant, DPA4 expression was slightly lower than in wild type
Engelhorn J, Reimer JJ, Leuz I, Göbel U, Huettel B, Farrona S, Turck F - Development-related PcG target in the apex 4 controls leaf margin architecture in Arabidopsis thaliana
Visit the PubMed ArticleCUC2 expression was strongly downregulated in 35S::DPA4-1 ... in ... young developing leaves, where no CUC2 transcript was detectable in 35S::DPA4 plants
Engelhorn J, Reimer JJ, Leuz I, Göbel U, Huettel B, Farrona S, Turck F - Development-related PcG target in the apex 4 controls leaf margin architecture in Arabidopsis thaliana
Visit the PubMed ArticleExpression analysis in the single ... mutants of ... DPA4 ... confirmed that ... CUC2 expression was increased
Engelhorn J, Reimer JJ, Leuz I, Göbel U, Huettel B, Farrona S, Turck F - Development-related PcG target in the apex 4 controls leaf margin architecture in Arabidopsis thaliana
Visit the PubMed ArticleCUC2 expression ... an additive increase was observed in mir164a-4/dpa4 double mutants
Engelhorn J, Reimer JJ, Leuz I, Göbel U, Huettel B, Farrona S, Turck F - Development-related PcG target in the apex 4 controls leaf margin architecture in Arabidopsis thaliana
Visit the PubMed ArticleDPA4 expression that might be conferred through chromatin-mediated gene repression by PcG proteins. We probed the expression of DPA4 in plants mutated in components of the PcG pathway. In the severe clf/swn double mutants, PcG-mediated repression is abolished in the developing seedling. DPA4 was ~6-fold upregulated at 22 days in clf/swn, whereas upregulation at 11 days was much weaker
Engelhorn J, Reimer JJ, Leuz I, Göbel U, Huettel B, Farrona S, Turck F - Development-related PcG target in the apex 4 controls leaf margin architecture in Arabidopsis thaliana
Visit the PubMed ArticleWe also analyzed RLT2 expression patterns by GUS staining of the RLT2pro:GUS transgenic plants ... RLT2 was expressed ubiquitously, and the most abundant expression was observed in the vigorously growing tissues
Li G, Zhang J, Li J, Yang Z, Huang H, Xu L - Imitation Switch chromatin remodeling factors and their interacting RINGLET proteins act together in controlling the plant vegetative phase in Arabidopsis
Visit the PubMed ArticleCHR17 ... Our quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis showed that the genes encoding AtISWI ... were universally expressed in different tissues
Li G, Zhang J, Li J, Yang Z, Huang H, Xu L - Imitation Switch chromatin remodeling factors and their interacting RINGLET proteins act together in controlling the plant vegetative phase in Arabidopsis
Visit the PubMed ArticleFT was actually up-regulated in the rlt1-1 rlt2-1double mutant
Li G, Zhang J, Li J, Yang Z, Huang H, Xu L - Imitation Switch chromatin remodeling factors and their interacting RINGLET proteins act together in controlling the plant vegetative phase in Arabidopsis
Visit the PubMed Articlesubcellular localization analyses showed that ... RLT2 ... mainly localized in the nucleus
Li G, Zhang J, Li J, Yang Z, Huang H, Xu L - Imitation Switch chromatin remodeling factors and their interacting RINGLET proteins act together in controlling the plant vegetative phase in Arabidopsis
Visit the PubMed ArticleRLT1 ... Our quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis showed that the genes encoding ... RLTs were universally expressed in different tissues
Li G, Zhang J, Li J, Yang Z, Huang H, Xu L - Imitation Switch chromatin remodeling factors and their interacting RINGLET proteins act together in controlling the plant vegetative phase in Arabidopsis
Visit the PubMed ArticleTo further investigate their expression patterns, we analyzed by in situ hybridization the transcript distributions of ... CHR17 ... CHR17 transcripts were detected in vigorously growing tissues ... These include the shoot apical meristem
Li G, Zhang J, Li J, Yang Z, Huang H, Xu L - Imitation Switch chromatin remodeling factors and their interacting RINGLET proteins act together in controlling the plant vegetative phase in Arabidopsis
Visit the PubMed Articlerlt1-1 rlt2-1 ... We further analyzed expression of SOC1 ... by qRT-PCR using 5-day-old seedlings and leaves from the 14-day-old seedlings, and our results showed that ... SOC1 were up-regulated
Li G, Zhang J, Li J, Yang Z, Huang H, Xu L - Imitation Switch chromatin remodeling factors and their interacting RINGLET proteins act together in controlling the plant vegetative phase in Arabidopsis
Visit the PubMed ArticleRLT2 ... Our quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis showed that the genes encoding ... RLTs were universally expressed in different tissues
Li G, Zhang J, Li J, Yang Z, Huang H, Xu L - Imitation Switch chromatin remodeling factors and their interacting RINGLET proteins act together in controlling the plant vegetative phase in Arabidopsis
Visit the PubMed ArticleSEP3 ... ectopically expressed in leaves of chr11-1 chr17-1
Li G, Zhang J, Li J, Yang Z, Huang H, Xu L - Imitation Switch chromatin remodeling factors and their interacting RINGLET proteins act together in controlling the plant vegetative phase in Arabidopsis
Visit the PubMed Articlerlt1-1 rlt2-1 ... We further analyzed expression of ... FUL ... by qRT-PCR using 5-day-old seedlings and leaves from the 14-day-old seedlings, and our results showed that FUL ... up-regulated
Li G, Zhang J, Li J, Yang Z, Huang H, Xu L - Imitation Switch chromatin remodeling factors and their interacting RINGLET proteins act together in controlling the plant vegetative phase in Arabidopsis
Visit the PubMed Articlechr11-1 chr17-1 ... We further analyzed expression of SOC1 ... by qRT-PCR using 5-day-old seedlings and leaves from the 14-day-old seedlings, and our results showed that ... SOC1 were up-regulated
Li G, Zhang J, Li J, Yang Z, Huang H, Xu L - Imitation Switch chromatin remodeling factors and their interacting RINGLET proteins act together in controlling the plant vegetative phase in Arabidopsis
Visit the PubMed Articlechr11-1 chr17-1 ... We further analyzed expression of ... FUL ... by qRT-PCR using 5-day-old seedlings and leaves from the 14-day-old seedlings, and our results showed that FUL ... up-regulated
Li G, Zhang J, Li J, Yang Z, Huang H, Xu L - Imitation Switch chromatin remodeling factors and their interacting RINGLET proteins act together in controlling the plant vegetative phase in Arabidopsis
Visit the PubMed ArticleCHR11 ... Our quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis showed that the genes encoding AtISWI ... were universally expressed in different tissues
Li G, Zhang J, Li J, Yang Z, Huang H, Xu L - Imitation Switch chromatin remodeling factors and their interacting RINGLET proteins act together in controlling the plant vegetative phase in Arabidopsis
Visit the PubMed ArticleCHR17 transcripts were detected in ... young leaves
Li G, Zhang J, Li J, Yang Z, Huang H, Xu L - Imitation Switch chromatin remodeling factors and their interacting RINGLET proteins act together in controlling the plant vegetative phase in Arabidopsis
Visit the PubMed ArticleSEP1 ... ectopically expressed in leaves of ... rlt1-1 rlt2-1
Li G, Zhang J, Li J, Yang Z, Huang H, Xu L - Imitation Switch chromatin remodeling factors and their interacting RINGLET proteins act together in controlling the plant vegetative phase in Arabidopsis
Visit the PubMed ArticleSEP1 ... ectopically expressed in leaves of chr11-1 chr17-1
Li G, Zhang J, Li J, Yang Z, Huang H, Xu L - Imitation Switch chromatin remodeling factors and their interacting RINGLET proteins act together in controlling the plant vegetative phase in Arabidopsis
Visit the PubMed Articlechr11-1 chr17-1 ... We further analyzed expression of ... FLC by qRT-PCR using 5-day-old seedlings and leaves from the 14-day-old seedlings, and our results showed that ... FLC was down-regulated
Li G, Zhang J, Li J, Yang Z, Huang H, Xu L - Imitation Switch chromatin remodeling factors and their interacting RINGLET proteins act together in controlling the plant vegetative phase in Arabidopsis
Visit the PubMed Articlesubcellular localization analyses showed that ... CHR11 ... mainly localized in the nucleus
Li G, Zhang J, Li J, Yang Z, Huang H, Xu L - Imitation Switch chromatin remodeling factors and their interacting RINGLET proteins act together in controlling the plant vegetative phase in Arabidopsis
Visit the PubMed ArticleCHR11 ... transcripts were detected in ... young leaves
Li G, Zhang J, Li J, Yang Z, Huang H, Xu L - Imitation Switch chromatin remodeling factors and their interacting RINGLET proteins act together in controlling the plant vegetative phase in Arabidopsis
Visit the PubMed ArticleTo further investigate their expression patterns, we analyzed by in situ hybridization the transcript distributions of CHR11 ... CHR11 ... transcripts were detected in vigorously growing tissues ... These include the shoot apical meristem
Li G, Zhang J, Li J, Yang Z, Huang H, Xu L - Imitation Switch chromatin remodeling factors and their interacting RINGLET proteins act together in controlling the plant vegetative phase in Arabidopsis
Visit the PubMed ArticleThe microarray analyses also showed that expression of the FT gene was up-regulated in chr11-1 chr17-1 ... However, our qRT-PCR analysis not only confirmed that this gene was up-regulated in chr11-1 chr17-1
Li G, Zhang J, Li J, Yang Z, Huang H, Xu L - Imitation Switch chromatin remodeling factors and their interacting RINGLET proteins act together in controlling the plant vegetative phase in Arabidopsis
Visit the PubMed ArticleTCP20-GFP signal was predominantly present in nuclei
Danisman S, van der Wal F, Dhondt S, Waites R, de Folter S, Bimbo A, van Dijk AD, Muino JM, Cutri L, Dornelas MC, Angenent GC, Immink RG - Arabidopsis class I and class II TCP transcription factors regulate jasmonic acid metabolism and leaf development antagonistically
Visit the PubMed Articleexpression levels of ... TCP20 ... were determined in four and 14-day-old leaves by real-time PCR ... This analysis shows that the ... TCP ... equally ... expressed in older leaves in comparison to young leaves
Danisman S, van der Wal F, Dhondt S, Waites R, de Folter S, Bimbo A, van Dijk AD, Muino JM, Cutri L, Dornelas MC, Angenent GC, Immink RG - Arabidopsis class I and class II TCP transcription factors regulate jasmonic acid metabolism and leaf development antagonistically
Visit the PubMed ArticlePlants expressing a genomic version of TCP20 tagged with a GREEN FLUORESCENT PROTEIN (GFP) encoding sequence under control of the endogenous TCP20 promoter (gTCP20-GFP) were analyzed at different time points after germination to obtain detailed information about the developmental expression pattern of TCP20. Fluorescence was detected by confocal laser scanning microscopy (CLSM) as shown in Figure 1. Expression was observed in several organs throughout development, like young and mature leaves
Danisman S, van der Wal F, Dhondt S, Waites R, de Folter S, Bimbo A, van Dijk AD, Muino JM, Cutri L, Dornelas MC, Angenent GC, Immink RG - Arabidopsis class I and class II TCP transcription factors regulate jasmonic acid metabolism and leaf development antagonistically
Visit the PubMed Articletcp20 knockout alone is not able to change TCP9 expression, suggesting that redundant factors activate TCP9 expression in the absence of TCP20
Danisman S, van der Wal F, Dhondt S, Waites R, de Folter S, Bimbo A, van Dijk AD, Muino JM, Cutri L, Dornelas MC, Angenent GC, Immink RG - Arabidopsis class I and class II TCP transcription factors regulate jasmonic acid metabolism and leaf development antagonistically
Visit the PubMed ArticleIn developing leaves, we detected TCP20-GFP signal in the first leaf primordia of seedlings three days after germination
Danisman S, van der Wal F, Dhondt S, Waites R, de Folter S, Bimbo A, van Dijk AD, Muino JM, Cutri L, Dornelas MC, Angenent GC, Immink RG - Arabidopsis class I and class II TCP transcription factors regulate jasmonic acid metabolism and leaf development antagonistically
Visit the PubMed ArticleLOX2 expression itself changes substantially during leaf development ... shows an increased expression in the first leaf 14 days after sowing
Danisman S, van der Wal F, Dhondt S, Waites R, de Folter S, Bimbo A, van Dijk AD, Muino JM, Cutri L, Dornelas MC, Angenent GC, Immink RG - Arabidopsis class I and class II TCP transcription factors regulate jasmonic acid metabolism and leaf development antagonistically
Visit the PubMed Articleexpression levels of ... TCP8 ... were determined in four and 14-day-old leaves by real-time PCR ... This analysis shows that the ... TCP ... equally ... expressed in older leaves in comparison to young leaves
Danisman S, van der Wal F, Dhondt S, Waites R, de Folter S, Bimbo A, van Dijk AD, Muino JM, Cutri L, Dornelas MC, Angenent GC, Immink RG - Arabidopsis class I and class II TCP transcription factors regulate jasmonic acid metabolism and leaf development antagonistically
Visit the PubMed Articleexpression levels of TCP4 ... were determined in four and 14-day-old leaves by real-time PCR ... the class II TCP4 gene is expressed stronger in old leaves, which is in line with an increased LOX2 expression in the 14-day-old leaves
Danisman S, van der Wal F, Dhondt S, Waites R, de Folter S, Bimbo A, van Dijk AD, Muino JM, Cutri L, Dornelas MC, Angenent GC, Immink RG - Arabidopsis class I and class II TCP transcription factors regulate jasmonic acid metabolism and leaf development antagonistically
Visit the PubMed ArticleTCP9 gets induced upon DEX induction of TCP20-GR in the tcp20 mutant background
Danisman S, van der Wal F, Dhondt S, Waites R, de Folter S, Bimbo A, van Dijk AD, Muino JM, Cutri L, Dornelas MC, Angenent GC, Immink RG - Arabidopsis class I and class II TCP transcription factors regulate jasmonic acid metabolism and leaf development antagonistically
Visit the PubMed Articlewe could detect significant LOX2 repression already two hours after Dexamethasone/Cycloheximide treatment (t-Test, p = 0.004 ... tcp20/35S:TCP20-GR plants
Danisman S, van der Wal F, Dhondt S, Waites R, de Folter S, Bimbo A, van Dijk AD, Muino JM, Cutri L, Dornelas MC, Angenent GC, Immink RG - Arabidopsis class I and class II TCP transcription factors regulate jasmonic acid metabolism and leaf development antagonistically
Visit the PubMed Articlewe detected TCP20-GFP signal ... in the first leaf of two ... to four weeks old plants
Danisman S, van der Wal F, Dhondt S, Waites R, de Folter S, Bimbo A, van Dijk AD, Muino JM, Cutri L, Dornelas MC, Angenent GC, Immink RG - Arabidopsis class I and class II TCP transcription factors regulate jasmonic acid metabolism and leaf development antagonistically
Visit the PubMed ArticleLOX2 expression itself changes substantially during leaf development ... it is lowly expressed during early leaf development
Danisman S, van der Wal F, Dhondt S, Waites R, de Folter S, Bimbo A, van Dijk AD, Muino JM, Cutri L, Dornelas MC, Angenent GC, Immink RG - Arabidopsis class I and class II TCP transcription factors regulate jasmonic acid metabolism and leaf development antagonistically
Visit the PubMed ArticleIn leaves of 14–day-old tcp20 mutant plants LOX2 expression was significantly up-regulated
Danisman S, van der Wal F, Dhondt S, Waites R, de Folter S, Bimbo A, van Dijk AD, Muino JM, Cutri L, Dornelas MC, Angenent GC, Immink RG - Arabidopsis class I and class II TCP transcription factors regulate jasmonic acid metabolism and leaf development antagonistically
Visit the PubMed Articleexpression levels of ... TCP22 were determined in four and 14-day-old leaves by real-time ... This analysis shows that the ... TCP ... lower ... expressed in older leaves in comparison to young leaves
Danisman S, van der Wal F, Dhondt S, Waites R, de Folter S, Bimbo A, van Dijk AD, Muino JM, Cutri L, Dornelas MC, Angenent GC, Immink RG - Arabidopsis class I and class II TCP transcription factors regulate jasmonic acid metabolism and leaf development antagonistically
Visit the PubMed ArticleIn the case of PIN3-GFP, most lines resulting from the cross of PIN3-GFP into the pin1 background showed expression similar to the wild-type background (Supplemental Fig. S3, B and F). However, some lines expressed PIN3-GFP in ... bulging primordia of ... stage III plants
Guenot B, Bayer E, Kierzkowski D, Smith RS, Mandel T, Žádníková P, Benková E, Kuhlemeier C - Pin1-independent leaf initiation in Arabidopsis
Visit the PubMed ArticleTherefore, we hypothesized that PIN proteins might likewise be up-regulated in the shoot meristem in the absence of PIN1. To address this question, we crossed all four GFP constructs into the pin1 background. In segregating populations of stage I seedlings, all analyzed ... PIN4-GFPpin1 ... meristems showed expression patterns comparable to wild-type populations
Guenot B, Bayer E, Kierzkowski D, Smith RS, Mandel T, Žádníková P, Benková E, Kuhlemeier C - Pin1-independent leaf initiation in Arabidopsis
Visit the PubMed ArticleIn stage I wild-type seedlings, PIN1-GFP was expressed in the ... vasculature of leaf primordia
Guenot B, Bayer E, Kierzkowski D, Smith RS, Mandel T, Žádníková P, Benková E, Kuhlemeier C - Pin1-independent leaf initiation in Arabidopsis
Visit the PubMed ArticleIn wild-type plants ... during stages II ... DR5-GFP was expressed in regularly spaced peaks in young and incipient primordia
Guenot B, Bayer E, Kierzkowski D, Smith RS, Mandel T, Žádníková P, Benková E, Kuhlemeier C - Pin1-independent leaf initiation in Arabidopsis
Visit the PubMed ArticlePIN2-GFP was expressed in a single row of leaf margin cells with polarization toward leaf tips
Guenot B, Bayer E, Kierzkowski D, Smith RS, Mandel T, Žádníková P, Benková E, Kuhlemeier C - Pin1-independent leaf initiation in Arabidopsis
Visit the PubMed ArticleIn contrast, DR5-GFP expression in pin1 clearly differed from the wild type in stage II ... Stage II meristems were often surrounded by very strong DR5-GFP signal
Guenot B, Bayer E, Kierzkowski D, Smith RS, Mandel T, Žádníková P, Benková E, Kuhlemeier C - Pin1-independent leaf initiation in Arabidopsis
Visit the PubMed ArticleIn stage III ... PIN1-GFP was the only PIN protein found in the meristem
Guenot B, Bayer E, Kierzkowski D, Smith RS, Mandel T, Žádníková P, Benková E, Kuhlemeier C - Pin1-independent leaf initiation in Arabidopsis
Visit the PubMed ArticleIn contrast, DR5-GFP expression in pin1 clearly differed from the wild type in stage II ... Stage II ... but DR5-GFP was usually not detectable in the meristem itself
Guenot B, Bayer E, Kierzkowski D, Smith RS, Mandel T, Žádníková P, Benková E, Kuhlemeier C - Pin1-independent leaf initiation in Arabidopsis
Visit the PubMed ArticleStage I wild-type seedlings showed DR5-GFP expression in leaf tips
Guenot B, Bayer E, Kierzkowski D, Smith RS, Mandel T, Žádníková P, Benková E, Kuhlemeier C - Pin1-independent leaf initiation in Arabidopsis
Visit the PubMed Articlewild-type plants ... during stages ... III ... DR5-GFP was expressed in regularly spaced peaks in young and incipient primordia
Guenot B, Bayer E, Kierzkowski D, Smith RS, Mandel T, Žádníková P, Benková E, Kuhlemeier C - Pin1-independent leaf initiation in Arabidopsis
Visit the PubMed ArticlePIN4 ... None of the other PIN-GFP constructs was detected in the meristem
Guenot B, Bayer E, Kierzkowski D, Smith RS, Mandel T, Žádníková P, Benková E, Kuhlemeier C - Pin1-independent leaf initiation in Arabidopsis
Visit the PubMed ArticleIn stage I wild-type seedlings, PIN1-GFP was expressed ... in the meristem itself
Guenot B, Bayer E, Kierzkowski D, Smith RS, Mandel T, Žádníková P, Benková E, Kuhlemeier C - Pin1-independent leaf initiation in Arabidopsis
Visit the PubMed ArticlePIN7 ... None of the other PIN-GFP constructs was detected in the meristem
Guenot B, Bayer E, Kierzkowski D, Smith RS, Mandel T, Žádníková P, Benková E, Kuhlemeier C - Pin1-independent leaf initiation in Arabidopsis
Visit the PubMed ArticleHowever, it was difficult to draw conclusions about leaf development in stage III plants because DR5-GFP was never visible in young primordia of mutant stage III meristems, suggesting that the DR5 promoter might not be responsive in those tissues
Guenot B, Bayer E, Kierzkowski D, Smith RS, Mandel T, Žádníková P, Benková E, Kuhlemeier C - Pin1-independent leaf initiation in Arabidopsis
Visit the PubMed ArticlePIN7-GFP was found in a pattern very similar to PIN3-GFP in hypocotyls
Guenot B, Bayer E, Kierzkowski D, Smith RS, Mandel T, Žádníková P, Benková E, Kuhlemeier C - Pin1-independent leaf initiation in Arabidopsis
Visit the PubMed ArticleTherefore, we hypothesized that PIN proteins might likewise be up-regulated in the shoot meristem in the absence of PIN1. To address this question, we crossed all four GFP constructs into the pin1 background. In segregating populations of stage I seedlings, all analyzed ... PIN7-GFPpin1 ... meristems showed expression patterns comparable to wild-type populations
Guenot B, Bayer E, Kierzkowski D, Smith RS, Mandel T, Žádníková P, Benková E, Kuhlemeier C - Pin1-independent leaf initiation in Arabidopsis
Visit the PubMed ArticleWe analyzed a large population of seedlings segregating for the pin1 allele, assuming that any change in DR5-GFP due to the loss of PIN1 should be visible in one-fourth of them. All analyzed seedlings showed similar DR5-GFP expression as wild-type seedlings (Fig. 4B). As in wild-type seedlings, 64% (27 of 42) of the seedlings segregating for the pin1 mutation had DR5-GFP signal in the meristem itself
Guenot B, Bayer E, Kierzkowski D, Smith RS, Mandel T, Žádníková P, Benková E, Kuhlemeier C - Pin1-independent leaf initiation in Arabidopsis
Visit the PubMed ArticlePIN3-GFP in bulging primordia of ... stage III plants ... ectopic expression ... was also observed in phenotypically wild-type plants of those crosses
Guenot B, Bayer E, Kierzkowski D, Smith RS, Mandel T, Žádníková P, Benková E, Kuhlemeier C - Pin1-independent leaf initiation in Arabidopsis
Visit the PubMed ArticlePIN3-GFP was found in the epidermis of leaf primordia
Guenot B, Bayer E, Kierzkowski D, Smith RS, Mandel T, Žádníková P, Benková E, Kuhlemeier C - Pin1-independent leaf initiation in Arabidopsis
Visit the PubMed ArticleTherefore, we hypothesized that PIN proteins might likewise be up-regulated in the shoot meristem in the absence of PIN1. To address this question, we crossed all four GFP constructs into the pin1 background. In segregating populations of stage I seedlings, all analyzed PIN2-GFPpin1 ... meristems showed expression patterns comparable to wild-type populations
Guenot B, Bayer E, Kierzkowski D, Smith RS, Mandel T, Žádníková P, Benková E, Kuhlemeier C - Pin1-independent leaf initiation in Arabidopsis
Visit the PubMed ArticleStage I wild-type seedlings showed DR5-GFP expression in ... early veins
Guenot B, Bayer E, Kierzkowski D, Smith RS, Mandel T, Žádníková P, Benková E, Kuhlemeier C - Pin1-independent leaf initiation in Arabidopsis
Visit the PubMed ArticlePIN3-GFP was found in the epidermis of ... cotyledons
Guenot B, Bayer E, Kierzkowski D, Smith RS, Mandel T, Žádníková P, Benková E, Kuhlemeier C - Pin1-independent leaf initiation in Arabidopsis
Visit the PubMed ArticlePIN2 ... translational GFP fusions under the control of their native promoters ... None of the other PIN-GFP constructs was detected in the meristem
Guenot B, Bayer E, Kierzkowski D, Smith RS, Mandel T, Žádníková P, Benková E, Kuhlemeier C - Pin1-independent leaf initiation in Arabidopsis
Visit the PubMed ArticlePIN7-GFP was found in a pattern very similar to PIN3-GFP in ... the epidermis of young leaves
Guenot B, Bayer E, Kierzkowski D, Smith RS, Mandel T, Žádníková P, Benková E, Kuhlemeier C - Pin1-independent leaf initiation in Arabidopsis
Visit the PubMed ArticlePIN7-GFP ... was not found in young primordia
Guenot B, Bayer E, Kierzkowski D, Smith RS, Mandel T, Žádníková P, Benková E, Kuhlemeier C - Pin1-independent leaf initiation in Arabidopsis
Visit the PubMed ArticleIn stage I wild-type seedlings, PIN1-GFP was expressed in the epidermis ... of leaf primordia
Guenot B, Bayer E, Kierzkowski D, Smith RS, Mandel T, Žádníková P, Benková E, Kuhlemeier C - Pin1-independent leaf initiation in Arabidopsis
Visit the PubMed ArticleApproximately 60% (10 of 17) of wild-type seedlings displayed DR5-GFP expression in the shoot apical meristem
Guenot B, Bayer E, Kierzkowski D, Smith RS, Mandel T, Žádníková P, Benková E, Kuhlemeier C - Pin1-independent leaf initiation in Arabidopsis
Visit the PubMed ArticleIn the case of PIN3-GFP, most lines resulting from the cross of PIN3-GFP into the pin1 background showed expression similar to the wild-type background (Supplemental Fig. S3, B and F). However, some lines expressed PIN3-GFP in bulging primordia of stage I
Guenot B, Bayer E, Kierzkowski D, Smith RS, Mandel T, Žádníková P, Benková E, Kuhlemeier C - Pin1-independent leaf initiation in Arabidopsis
Visit the PubMed ArticleStage I wild-type seedlings showed DR5-GFP expression in ... stipules
Guenot B, Bayer E, Kierzkowski D, Smith RS, Mandel T, Žádníková P, Benková E, Kuhlemeier C - Pin1-independent leaf initiation in Arabidopsis
Visit the PubMed Articleunaltered expression of the central zone marker CLAVATA3-GFP in pin1 ... stage III
Guenot B, Bayer E, Kierzkowski D, Smith RS, Mandel T, Žádníková P, Benková E, Kuhlemeier C - Pin1-independent leaf initiation in Arabidopsis
Visit the PubMed ArticleIn contrast, DR5-GFP expression in pin1 clearly differed from the wild type in stage ... III mutants ... During stage III, DR5-GFP levels were again very high in the tissue subjacent to the meristem
Guenot B, Bayer E, Kierzkowski D, Smith RS, Mandel T, Žádníková P, Benková E, Kuhlemeier C - Pin1-independent leaf initiation in Arabidopsis
Visit the PubMed ArticleIn stage III ... PIN1-GFP was the only PIN protein found in ... young primordia
Guenot B, Bayer E, Kierzkowski D, Smith RS, Mandel T, Žádníková P, Benková E, Kuhlemeier C - Pin1-independent leaf initiation in Arabidopsis
Visit the PubMed ArticlePIN4-GFP was occasionally expressed very weakly in the epidermis of primordia
Guenot B, Bayer E, Kierzkowski D, Smith RS, Mandel T, Žádníková P, Benková E, Kuhlemeier C - Pin1-independent leaf initiation in Arabidopsis
Visit the PubMed ArticlePIN3 ... translational GFP fusions under the control of their native promoters ... None of the other PIN-GFP constructs was detected in the meristem
Guenot B, Bayer E, Kierzkowski D, Smith RS, Mandel T, Žádníková P, Benková E, Kuhlemeier C - Pin1-independent leaf initiation in Arabidopsis
Visit the PubMed ArticlePIN3-GFP in bulging primordia of stage I ... ectopic expression ... was also observed in phenotypically wild-type plants of those crosses
Guenot B, Bayer E, Kierzkowski D, Smith RS, Mandel T, Žádníková P, Benková E, Kuhlemeier C - Pin1-independent leaf initiation in Arabidopsis
Visit the PubMed Articlewe analyzed wounded ... plants to determine whether ... HPL expression levels are modulated in response to abiotic stresses ... These data show ... elevated HPL expression in plants treated with these abiotic stresses as compared to untreated controls
Xiao Y, Savchenko T, Baidoo EE, Chehab WE, Hayden DM, Tolstikov V, Corwin JA, Kliebenstein DJ, Keasling JD, Dehesh K - Retrograde signaling by the plastidial metabolite MEcPP regulates expression of nuclear stress-response genes
Visit the PubMed Articlethe expression levels of allene oxide synthase (AOS), a stress-inducible nuclear gene encoding a key plastidlocalized protein in the JA-biosynthetic pathway ... were comparable between ceh1 and control lines
Xiao Y, Savchenko T, Baidoo EE, Chehab WE, Hayden DM, Tolstikov V, Corwin JA, Kliebenstein DJ, Keasling JD, Dehesh K - Retrograde signaling by the plastidial metabolite MEcPP regulates expression of nuclear stress-response genes
Visit the PubMed ArticleTo examine the specificity of MEcPP as an inducer of the HPL expression, we exogenously treated the P lines with water as the control or with MEcPP before and immediately after wounding, followed by qRT-PCR analysis in conjunction with quantitative LUC bioluminescence measurements 90 min after these applications ... These measurements establish that the HPL expression is induced in response to exogenous application of MEcPP before or after wounding. However, the highest levels of HPL induction are displayed by plants treated with MEcPP postwounding, suggesting that ease of penetration through the wound site potentially enhanced accessibility of this metabolite
Xiao Y, Savchenko T, Baidoo EE, Chehab WE, Hayden DM, Tolstikov V, Corwin JA, Kliebenstein DJ, Keasling JD, Dehesh K - Retrograde signaling by the plastidial metabolite MEcPP regulates expression of nuclear stress-response genes
Visit the PubMed ArticleTo determine whether ceh1 affects similar mechanisms in plastid development to those affected by the gun mutants, we examined the expression levels of LHCB in wild-type (WT) and ceh1 seedlings grown in the presence of norflurazon (NF), an inhibitor of phytoene desaturase, a critical enzyme in the carotenoid biosynthesis pathway ... These data demonstrate that NF-treated WT and ceh1 seedlings are both photo bleached and have reduced levels of LHCB transcripts
Xiao Y, Savchenko T, Baidoo EE, Chehab WE, Hayden DM, Tolstikov V, Corwin JA, Kliebenstein DJ, Keasling JD, Dehesh K - Retrograde signaling by the plastidial metabolite MEcPP regulates expression of nuclear stress-response genes
Visit the PubMed ArticleAs the basis of the screen, transgenic plants were established that contain the HPL promoter region fused to the firefly luciferase (PHPL::LUC ... high and constitutive LUC activity levels detected ... in the ceh1/eds16-1
Xiao Y, Savchenko T, Baidoo EE, Chehab WE, Hayden DM, Tolstikov V, Corwin JA, Kliebenstein DJ, Keasling JD, Dehesh K - Retrograde signaling by the plastidial metabolite MEcPP regulates expression of nuclear stress-response genes
Visit the PubMed ArticleThis higher SA level in ceh1 was accompanied by an _4-fold increase in transcript levels of ICS1, a stress-inducible nuclear gene encoding a key plastidial enzyme in the SA-biosynthetic pathway
Xiao Y, Savchenko T, Baidoo EE, Chehab WE, Hayden DM, Tolstikov V, Corwin JA, Kliebenstein DJ, Keasling JD, Dehesh K - Retrograde signaling by the plastidial metabolite MEcPP regulates expression of nuclear stress-response genes
Visit the PubMed ArticleTo dissect the upstream signal transduction machinery responsible for stress induction of HPL, we devised a genetic screen to identify mutations that result in constitutive expression of HPL. As the basis of the screen, transgenic plants were established that contain the HPL promoter region fused to the firefly luciferase (PHPL::LUC) ... Ceh1 displayed a _100-fold higher LUC activity than the P control (Figure 1C). Correspondingly, qRT-PCR analysis showed_17-fold higher levels of the endogenous HPL transcript in ceh1 as compared to controls
Xiao Y, Savchenko T, Baidoo EE, Chehab WE, Hayden DM, Tolstikov V, Corwin JA, Kliebenstein DJ, Keasling JD, Dehesh K - Retrograde signaling by the plastidial metabolite MEcPP regulates expression of nuclear stress-response genes
Visit the PubMed Articlelack of any detectable changes in the expression levels of AOS in response to MEcPP treatment
Xiao Y, Savchenko T, Baidoo EE, Chehab WE, Hayden DM, Tolstikov V, Corwin JA, Kliebenstein DJ, Keasling JD, Dehesh K - Retrograde signaling by the plastidial metabolite MEcPP regulates expression of nuclear stress-response genes
Visit the PubMed Articlewe analyzed ... high-light-treated plants to determine ... HPL expression levels are modulated in response to abiotic stresses ... These data show ... elevated HPL expression in plants treated with these abiotic stresses as compared to untreated controls
Xiao Y, Savchenko T, Baidoo EE, Chehab WE, Hayden DM, Tolstikov V, Corwin JA, Kliebenstein DJ, Keasling JD, Dehesh K - Retrograde signaling by the plastidial metabolite MEcPP regulates expression of nuclear stress-response genes
Visit the PubMed ArticleTo characterize the pattern of EXPA gene expression, leaves at different developmental stages were dissected, RNA extracted and the presence/absence of individual expansin transcripts assayed by RT-PCR (Fig. 1D). The earliest stage of leaf development analyzed here (12-14 DAS) was characterized by the expression of at least 7 EXPA genes. These showed a general pattern of maintained expression throughout development, with other EXPA transcripts becoming detectable at later stages of development with a maximum number of up to 10 EXPA genes being expressed over a broad period from 20-28 DAS. This period of maximum number of expressed EXPA genes correlates with the period of maximum absolute rate (Fig. 1B) and the hiatus in relative rate of leaf expansion (Fig. 1C
Goh HH, Sloan J, Dorca-Fornell C, Fleming A - Inducible repression of multiple expansin genes leads to growth suppression during leaf development
Visit the PubMed ArticleTo characterize the pattern of EXPA gene expression, leaves at different developmental stages were dissected, RNA extracted and the presence/absence of individual expansin transcripts assayed by RT-PCR (Fig. 1D). The earliest stage of leaf development analyzed here (12-14 DAS) was characterized by the expression of at least 7 EXPA genes. These showed a general pattern of maintained expression throughout development, with other EXPA transcripts becoming detectable at later stages of development with a maximum number of up to 10 EXPA genes being expressed over a broad period from 20-28 DAS. This period of maximum number of expressed EXPA genes correlates with the period of maximum absolute rate (Fig. 1B) and the hiatus in relative rate of leaf expansion (Fig. 1C
Goh HH, Sloan J, Dorca-Fornell C, Fleming A - Inducible repression of multiple expansin genes leads to growth suppression during leaf development
Visit the PubMed ArticleTo characterize the pattern of EXPA gene expression, leaves at different developmental stages were dissected, RNA extracted and the presence/absence of individual expansin transcripts assayed by RT-PCR (Fig. 1D). The earliest stage of leaf development analyzed here (12-14 DAS) was characterized by the expression of at least 7 EXPA genes. These showed a general pattern of maintained expression throughout development, with other EXPA transcripts becoming detectable at later stages of development with a maximum number of up to 10 EXPA genes being expressed over a broad period from 20-28 DAS ... This period of maximum number of expressed EXPA genes correlates with the period of maximum absolute rate (Fig. 1B) and the hiatus in relative rate of leaf expansion (Fig. 1C
Goh HH, Sloan J, Dorca-Fornell C, Fleming A - Inducible repression of multiple expansin genes leads to growth suppression during leaf development
Visit the PubMed ArticleTo characterize the pattern of EXPA gene expression, leaves at different developmental stages were dissected, RNA extracted and the presence/absence of individual expansin transcripts assayed by RT-PCR (Fig. 1D). The earliest stage of leaf development analyzed here (12-14 DAS) was characterized by the expression of at least 7 EXPA genes. These showed a general pattern of maintained expression throughout development, with other EXPA transcripts becoming detectable at later stages of development with a maximum number of up to 10 EXPA genes being expressed over a broad period from 20-28 DAS. This period of maximum number of expressed EXPA genes correlates with the period of maximum absolute rate (Fig. 1B) and the hiatus in relative rate of leaf expansion (Fig. 1C
Goh HH, Sloan J, Dorca-Fornell C, Fleming A - Inducible repression of multiple expansin genes leads to growth suppression during leaf development
Visit the PubMed ArticleTo characterize the pattern of EXPA gene expression, leaves at different developmental stages were dissected, RNA extracted and the presence/absence of individual expansin transcripts assayed by RT-PCR (Fig. 1D). The earliest stage of leaf development analyzed here (12-14 DAS) was characterized by the expression of at least 7 EXPA genes. These showed a general pattern of maintained expression throughout development, with other EXPA transcripts becoming detectable at later stages of development with a maximum number of up to 10 EXPA genes being expressed over a broad period from 20-28 DAS. This period of maximum number of expressed EXPA genes correlates with the period of maximum absolute rate (Fig. 1B) and the hiatus in relative rate of leaf expansion (Fig. 1C
Goh HH, Sloan J, Dorca-Fornell C, Fleming A - Inducible repression of multiple expansin genes leads to growth suppression during leaf development
Visit the PubMed ArticleTo characterize the pattern of EXPA gene expression, leaves at different developmental stages were dissected, RNA extracted and the presence/absence of individual expansin transcripts assayed by RT-PCR (Fig. 1D). The earliest stage of leaf development analyzed here (12-14 DAS) was characterized by the expression of at least 7 EXPA genes. These showed a general pattern of maintained expression throughout development, with other EXPA transcripts becoming detectable at later stages of development with a maximum number of up to 10 EXPA genes being expressed over a broad period from 20-28 DAS. This period of maximum number of expressed EXPA genes correlates with the period of maximum absolute rate (Fig. 1B) and the hiatus in relative rate of leaf expansion (Fig. 1C
Goh HH, Sloan J, Dorca-Fornell C, Fleming A - Inducible repression of multiple expansin genes leads to growth suppression during leaf development
Visit the PubMed ArticleTo characterize the pattern of EXPA gene expression, leaves at different developmental stages were dissected, RNA extracted and the presence/absence of individual expansin transcripts assayed by RT-PCR (Fig. 1D). The earliest stage of leaf development analyzed here (12-14 DAS) was characterized by the expression of at least 7 EXPA genes. These showed a general pattern of maintained expression throughout development, with other EXPA transcripts becoming detectable at later stages of development with a maximum number of up to 10 EXPA genes being expressed over a broad period from 20-28 DAS. This period of maximum number of expressed EXPA genes correlates with the period of maximum absolute rate (Fig. 1B) and the hiatus in relative rate of leaf expansion (Fig. 1C
Goh HH, Sloan J, Dorca-Fornell C, Fleming A - Inducible repression of multiple expansin genes leads to growth suppression during leaf development
Visit the PubMed ArticleTo characterize the pattern of EXPA gene expression, leaves at different developmental stages were dissected, RNA extracted and the presence/absence of individual expansin transcripts assayed by RT-PCR (Fig. 1D). The earliest stage of leaf development analyzed here (12-14 DAS) was characterized by the expression of at least 7 EXPA genes ... These showed a general pattern of maintained expression throughout development, with other EXPA transcripts becoming detectable at later stages of development with a maximum number of up to 10 EXPA genes being expressed over a broad period from 20-28 DAS. This period of maximum number of expressed EXPA genes correlates with the period of maximum absolute rate (Fig. 1B) and the hiatus in relative rate of leaf expansion (Fig. 1C
Goh HH, Sloan J, Dorca-Fornell C, Fleming A - Inducible repression of multiple expansin genes leads to growth suppression during leaf development
Visit the PubMed ArticleTo characterize the pattern of EXPA gene expression, leaves at different developmental stages were dissected, RNA extracted and the presence/absence of individual expansin transcripts assayed by RT-PCR (Fig. 1D). The earliest stage of leaf development analyzed here (12-14 DAS) was characterized by the expression of at least 7 EXPA genes. These showed a general pattern of maintained expression throughout development, with other EXPA transcripts becoming detectable at later stages of development with a maximum number of up to 10 EXPA genes being expressed over a broad period from 20-28 DAS. This period of maximum number of expressed EXPA genes correlates with the period of maximum absolute rate (Fig. 1B) and the hiatus in relative rate of leaf expansion (Fig. 1C
Goh HH, Sloan J, Dorca-Fornell C, Fleming A - Inducible repression of multiple expansin genes leads to growth suppression during leaf development
Visit the PubMed ArticleRNA from 7-day old ... seedlings ... were subjected to quantitative real-time RT-PCR (qRT-PCR) analysis (Figure 4 ... in ... fln1-1 ... Transcripts for ... rpoC1 ... were slightly ... slightly elevated
Gilkerson J, Perez-Ruiz JM, Chory J, Callis J - The plastid-localized pfkB-type carbohydrate kinases FRUCTOKINASE-LIKE 1 and 2 are essential for growth and development of Arabidopsis thaliana
Visit the PubMed ArticleRNA from 7-day old ... seedlings ... were subjected to quantitative real-time RT-PCR (qRT-PCR) analysis ... in ... fln2-1 ... for rpl23 ... transcripts ... only 50% of wild type expression
Gilkerson J, Perez-Ruiz JM, Chory J, Callis J - The plastid-localized pfkB-type carbohydrate kinases FRUCTOKINASE-LIKE 1 and 2 are essential for growth and development of Arabidopsis thaliana
Visit the PubMed ArticleRNA from 7-day old ... seedlings ... were subjected to quantitative real-time RT-PCR (qRT-PCR) analysis ... in ... fln2-1 ... clpP ... mRNAs were slightly up-regulated
Gilkerson J, Perez-Ruiz JM, Chory J, Callis J - The plastid-localized pfkB-type carbohydrate kinases FRUCTOKINASE-LIKE 1 and 2 are essential for growth and development of Arabidopsis thaliana
Visit the PubMed ArticleRNA from 7-day old ... seedlings ... were subjected to ... quantitative real-time RT-PCR (qRT-PCR) analysis ... Expression of ... psbA ... was ... strongly reduced in ... fln1-1 ... seedlings compared to Col-0 and to their respective phenotypically wild-type siblings
Gilkerson J, Perez-Ruiz JM, Chory J, Callis J - The plastid-localized pfkB-type carbohydrate kinases FRUCTOKINASE-LIKE 1 and 2 are essential for growth and development of Arabidopsis thaliana
Visit the PubMed ArticleRNA from 7-day old ... seedlings ... were subjected to quantitative real-time RT-PCR (qRT-PCR) analysis ... Expression of ... psbB ... was strongly reduced in ... fln1-1 ... fln1 ... seedlings compared to Col-0 and to their respective phenotypically wild-type siblings
Gilkerson J, Perez-Ruiz JM, Chory J, Callis J - The plastid-localized pfkB-type carbohydrate kinases FRUCTOKINASE-LIKE 1 and 2 are essential for growth and development of Arabidopsis thaliana
Visit the PubMed ArticleRNA from 7-day old ... seedlings ... were subjected to quantitative real-time RT-PCR (qRT-PCR) analysis ... in ... fln1-1 ... ndhB mRNAs ... ndhB ... were slightly up-regulated
Gilkerson J, Perez-Ruiz JM, Chory J, Callis J - The plastid-localized pfkB-type carbohydrate kinases FRUCTOKINASE-LIKE 1 and 2 are essential for growth and development of Arabidopsis thaliana
Visit the PubMed ArticleRNA from 7-day old ... seedlings ... were subjected to quantitative real-time RT-PCR (qRT-PCR) analysis ... in ... fln2-1 ... Transcripts for ... accD ... were slightly elevated
Gilkerson J, Perez-Ruiz JM, Chory J, Callis J - The plastid-localized pfkB-type carbohydrate kinases FRUCTOKINASE-LIKE 1 and 2 are essential for growth and development of Arabidopsis thaliana
Visit the PubMed ArticleRNA from 7-day old ... seedlings ... were subjected to quantitative real-time RT-PCR (qRT-PCR) analysis ... in ... fln1-1 ... Transcripts for ... rpoB ... were slightly elevated
Gilkerson J, Perez-Ruiz JM, Chory J, Callis J - The plastid-localized pfkB-type carbohydrate kinases FRUCTOKINASE-LIKE 1 and 2 are essential for growth and development of Arabidopsis thaliana
Visit the PubMed ArticleRNA from 7-day old ... seedlings ... were subjected to quantitative real-time RT-PCR (qRT-PCR) analysis ... in ... fln1-1 ... fln1 ... fln1 ... clpP ... were slightly up-regulated
Gilkerson J, Perez-Ruiz JM, Chory J, Callis J - The plastid-localized pfkB-type carbohydrate kinases FRUCTOKINASE-LIKE 1 and 2 are essential for growth and development of Arabidopsis thaliana
Visit the PubMed ArticleRNA from 7-day old ... seedlings ... were subjected to quantitative real-time RT-PCR (qRT-PCR) analysis ... in ... fln2-1 ... Transcripts for ... rpoB ... were slightly ... slightly elevated ... elevated
Gilkerson J, Perez-Ruiz JM, Chory J, Callis J - The plastid-localized pfkB-type carbohydrate kinases FRUCTOKINASE-LIKE 1 and 2 are essential for growth and development of Arabidopsis thaliana
Visit the PubMed ArticleRNA from 7-day old ... seedlings ... were subjected to quantitative real-time RT-PCR (qRT-PCR) analysis ... Expression of ... psbA ... was strongly reduced in ... fln2-1 seedlings compared to Col-0 and to their respective phenotypically wild-type siblings
Gilkerson J, Perez-Ruiz JM, Chory J, Callis J - The plastid-localized pfkB-type carbohydrate kinases FRUCTOKINASE-LIKE 1 and 2 are essential for growth and development of Arabidopsis thaliana
Visit the PubMed ArticleRNA from 7-day old ... seedlings ... were subjected to ... quantitative real-time RT-PCR (qRT-PCR) analysis ... Expression of ... rrn23S was strongly reduced in ... rrn23S ... fln1-1 ... seedlings compared to Col-0 and to their respective phenotypically wild-type siblings
Gilkerson J, Perez-Ruiz JM, Chory J, Callis J - The plastid-localized pfkB-type carbohydrate kinases FRUCTOKINASE-LIKE 1 and 2 are essential for growth and development of Arabidopsis thaliana
Visit the PubMed ArticleRNA from 7-day old ... seedlings ... were subjected to quantitative real-time RT-PCR (qRT-PCR) analysis ... Expression of ... Expression ... rbcL ... was strongly reduced in both fln1-1 ... seedlings compared to Col-0 ... 0 and ... and to their respective phenotypically wild-type siblings
Gilkerson J, Perez-Ruiz JM, Chory J, Callis J - The plastid-localized pfkB-type carbohydrate kinases FRUCTOKINASE-LIKE 1 and 2 are essential for growth and development of Arabidopsis thaliana
Visit the PubMed ArticleRNA from 7-day old ... seedlings ... were subjected to quantitative real-time RT-PCR (qRT-PCR) analysis ... FLN1 mRNA is not elevated in fln2-1
Gilkerson J, Perez-Ruiz JM, Chory J, Callis J - The plastid-localized pfkB-type carbohydrate kinases FRUCTOKINASE-LIKE 1 and 2 are essential for growth and development of Arabidopsis thaliana
Visit the PubMed ArticleRNA from 7-day old ... seedlings ... were subjected to ... quantitative real-time RT-PCR (qRT-PCR) analysis ... Expression of ... rrn23S ... was strongly reduced in ... fln2-1 seedlings compared to Col-0 and to their respective phenotypically wild-type siblings
Gilkerson J, Perez-Ruiz JM, Chory J, Callis J - The plastid-localized pfkB-type carbohydrate kinases FRUCTOKINASE-LIKE 1 and 2 are essential for growth and development of Arabidopsis thaliana
Visit the PubMed ArticleRNA from 7-day ... day old ... old ... seedlings ... were subjected ... subjected to ... quantitative real-time RT-PCR (qRT-PCR) analysis ... Expression of ... rbcL ... was strongly reduced in ... fln2-1 seedlings compared to Col-0 and to their respective phenotypically wild-type siblings
Gilkerson J, Perez-Ruiz JM, Chory J, Callis J - The plastid-localized pfkB-type carbohydrate kinases FRUCTOKINASE-LIKE 1 and 2 are essential for growth and development of Arabidopsis thaliana
Visit the PubMed ArticleRNA from 7-day old ... seedlings ... were subjected to quantitative real-time RT-PCR (qRT-PCR) analysis ... in ... fln1-1 ... Transcripts for ... accD ... were slightly elevated
Gilkerson J, Perez-Ruiz JM, Chory J, Callis J - The plastid-localized pfkB-type carbohydrate kinases FRUCTOKINASE-LIKE 1 and 2 are essential for growth and development of Arabidopsis thaliana
Visit the PubMed ArticleRNA from 7-day old ... seedlings ... were subjected to quantitative real-time RT-PCR (qRT-PCR) analysis ... in ... fln1-1 ... for rpl23 ... transcripts ... only 50% of wild type expression
Gilkerson J, Perez-Ruiz JM, Chory J, Callis J - The plastid-localized pfkB-type carbohydrate kinases FRUCTOKINASE-LIKE 1 and 2 are essential for growth and development of Arabidopsis thaliana
Visit the PubMed ArticleRNA from 7-day old ... seedlings ... were subjected to quantitative real-time RT-PCR (qRT-PCR) analysis ... in ... fln2-1 ... ndhB mRNAs were slightly up-regulated
Gilkerson J, Perez-Ruiz JM, Chory J, Callis J - The plastid-localized pfkB-type carbohydrate kinases FRUCTOKINASE-LIKE 1 and 2 are essential for growth and development of Arabidopsis thaliana
Visit the PubMed ArticleRNA from 7-day old ... seedlings ... were subjected to quantitative real-time RT-PCR (qRT-PCR) analysis ... FLN2 mRNA is two-fold higher in fln1-1 than Col-0,
Gilkerson J, Perez-Ruiz JM, Chory J, Callis J - The plastid-localized pfkB-type carbohydrate kinases FRUCTOKINASE-LIKE 1 and 2 are essential for growth and development of Arabidopsis thaliana
Visit the PubMed ArticleRNA from 7-day old ... seedlings ... were subjected to ... quantitative real-time RT-PCR (qRT-PCR) analysis ... Expression of ... psbB ... was strongly reduced in ... fln2-1 seedlings compared to Col-0 and to their respective phenotypically wild-type siblings
Gilkerson J, Perez-Ruiz JM, Chory J, Callis J - The plastid-localized pfkB-type carbohydrate kinases FRUCTOKINASE-LIKE 1 and 2 are essential for growth and development of Arabidopsis thaliana
Visit the PubMed ArticleRNA from 7-day old ... seedlings ... were subjected to quantitative real-time RT-PCR (qRT-PCR) analysis ... Transcripts for ... rpoC1 ... were slightly ... were ... slightly elevated
Gilkerson J, Perez-Ruiz JM, Chory J, Callis J - The plastid-localized pfkB-type carbohydrate kinases FRUCTOKINASE-LIKE 1 and 2 are essential for growth and development of Arabidopsis thaliana
Visit the PubMed Articleusing the genes coding for the ER-located ... Bip ... 2 ... exhibit markedly increased expression levels in pht4;6 plants compared to wild-type plants
Hassler S, Lemke L, Jung B, Möhlmann T, Krüger F, Schumacher K, Espen L, Martinoia E, Neuhaus HE - Lack of the Golgi phosphate transporter PHT4;6 causes strong developmental defects, constitutively activated disease resistance mechanisms and altered intracellular phosphate compartmentation in Arabidopsis
Visit the PubMed Articlethe 3′(2′),5′-bisphosphate nucleotidase SAL2 (At5g64000) ... In leaves of pht4;6 mutants, all four Pi-responsive genes were drastically (4.5–19-fold) up-regulated compared to wild-type plants
Hassler S, Lemke L, Jung B, Möhlmann T, Krüger F, Schumacher K, Espen L, Martinoia E, Neuhaus HE - Lack of the Golgi phosphate transporter PHT4;6 causes strong developmental defects, constitutively activated disease resistance mechanisms and altered intracellular phosphate compartmentation in Arabidopsis
Visit the PubMed ArticlePR1 (an SA-induced gene of unclear function) ... exhibit increased expression in pht4;6 plants compared to corresponding wild-type plants
Hassler S, Lemke L, Jung B, Möhlmann T, Krüger F, Schumacher K, Espen L, Martinoia E, Neuhaus HE - Lack of the Golgi phosphate transporter PHT4;6 causes strong developmental defects, constitutively activated disease resistance mechanisms and altered intracellular phosphate compartmentation in Arabidopsis
Visit the PubMed ArticleSome types of sugar moieties required for hemicellulose biosynthesis also serve in covalent modification of proteins in the ER and Golgi compartments. Therefore, we also searched for putative modifications in the protein glycosylation pattern. Using concanavalin A, a lectin that is able to bind high-mannose-type N-glycans attached to polypeptides (Mandal and Brewer, 1993), we demonstrated that at least three proteins of unknown nature show altered abundance in pht4;6 leaves compared to wild-type plants
Hassler S, Lemke L, Jung B, Möhlmann T, Krüger F, Schumacher K, Espen L, Martinoia E, Neuhaus HE - Lack of the Golgi phosphate transporter PHT4;6 causes strong developmental defects, constitutively activated disease resistance mechanisms and altered intracellular phosphate compartmentation in Arabidopsis
Visit the PubMed ArticlePAL (encoding phenylalanine ammonia- lyase) ... exhibit increased expression in pht4;6 plants compared to corresponding wild-type plants
Hassler S, Lemke L, Jung B, Möhlmann T, Krüger F, Schumacher K, Espen L, Martinoia E, Neuhaus HE - Lack of the Golgi phosphate transporter PHT4;6 causes strong developmental defects, constitutively activated disease resistance mechanisms and altered intracellular phosphate compartmentation in Arabidopsis
Visit the PubMed ArticleWRKY38 ... In leaves of pht4;6 mutants, all four Pi-responsive genes were drastically (4.5–19-fold) up-regulated compared to wild-type plants
Hassler S, Lemke L, Jung B, Möhlmann T, Krüger F, Schumacher K, Espen L, Martinoia E, Neuhaus HE - Lack of the Golgi phosphate transporter PHT4;6 causes strong developmental defects, constitutively activated disease resistance mechanisms and altered intracellular phosphate compartmentation in Arabidopsis
Visit the PubMed ArticleGSTF6 (a glutathione transferase) ... exhibit increased expression in pht4;6 plants compared to corresponding wild-type plants
Hassler S, Lemke L, Jung B, Möhlmann T, Krüger F, Schumacher K, Espen L, Martinoia E, Neuhaus HE - Lack of the Golgi phosphate transporter PHT4;6 causes strong developmental defects, constitutively activated disease resistance mechanisms and altered intracellular phosphate compartmentation in Arabidopsis
Visit the PubMed Articleusing the genes coding for the ER-located ... calreticulin1 ... exhibit markedly increased expression levels in pht4;6 plants compared to wild-type plants
Hassler S, Lemke L, Jung B, Möhlmann T, Krüger F, Schumacher K, Espen L, Martinoia E, Neuhaus HE - Lack of the Golgi phosphate transporter PHT4;6 causes strong developmental defects, constitutively activated disease resistance mechanisms and altered intracellular phosphate compartmentation in Arabidopsis
Visit the PubMed Articlethe transcription factor BZIP60 ... exhibit markedly increased expression levels in pht4;6 plants compared to wild-type plants
Hassler S, Lemke L, Jung B, Möhlmann T, Krüger F, Schumacher K, Espen L, Martinoia E, Neuhaus HE - Lack of the Golgi phosphate transporter PHT4;6 causes strong developmental defects, constitutively activated disease resistance mechanisms and altered intracellular phosphate compartmentation in Arabidopsis
Visit the PubMed Articleusing the genes coding for the ER-located ... Bip3 ... exhibit markedly increased expression levels in pht4;6 plants compared to wild-type plants
Hassler S, Lemke L, Jung B, Möhlmann T, Krüger F, Schumacher K, Espen L, Martinoia E, Neuhaus HE - Lack of the Golgi phosphate transporter PHT4;6 causes strong developmental defects, constitutively activated disease resistance mechanisms and altered intracellular phosphate compartmentation in Arabidopsis
Visit the PubMed ArticlePHT4;6 locates to the Golgi apparatus (Guo et al., 2008b; Cubero et al., 2009), but the exact location within this very complex cellular structure is unknown. Using co-expression of a PHT4;6–GFP construct with either cis-Golgi-located α-mannosidase–RFP (Nebenführ et al., 2002) or trans-Golgi-located sialin transferase–DsRFP (ST–DsRFP) (Saint-Jore et al., 2002), we were able to clarify the subcellular location of PHT4;6 in more detail. A merged image of PHT4;6–GFP with α-mannosidase–RFP at high magnification showed distinctly labeled sub-domains of the Golgi apparatus, whereas a merged image of PHT4;6–GFP with ST–DsRed revealed close overlap, indicating a trans-Golgi location of the PHT4;6 protein
Hassler S, Lemke L, Jung B, Möhlmann T, Krüger F, Schumacher K, Espen L, Martinoia E, Neuhaus HE - Lack of the Golgi phosphate transporter PHT4;6 causes strong developmental defects, constitutively activated disease resistance mechanisms and altered intracellular phosphate compartmentation in Arabidopsis
Visit the PubMed Articleusing the genes coding for the ER-located ... calreticulin ... 2 ... exhibit markedly increased expression levels in pht4;6 plants compared to wild-type plants
Hassler S, Lemke L, Jung B, Möhlmann T, Krüger F, Schumacher K, Espen L, Martinoia E, Neuhaus HE - Lack of the Golgi phosphate transporter PHT4;6 causes strong developmental defects, constitutively activated disease resistance mechanisms and altered intracellular phosphate compartmentation in Arabidopsis
Visit the PubMed Articlethe plasma membrane-located phosphate importer PHT1;4 ... In leaves of pht4;6 mutants, all four Pi-responsive genes were drastically (4.5–19-fold) up-regulated compared to wild-type plants
Hassler S, Lemke L, Jung B, Möhlmann T, Krüger F, Schumacher K, Espen L, Martinoia E, Neuhaus HE - Lack of the Golgi phosphate transporter PHT4;6 causes strong developmental defects, constitutively activated disease resistance mechanisms and altered intracellular phosphate compartmentation in Arabidopsis
Visit the PubMed ArticlePR2 (ß-1,3--glucanase) ... exhibit increased expression in pht4;6 plants compared to corresponding wild-type plants
Hassler S, Lemke L, Jung B, Möhlmann T, Krüger F, Schumacher K, Espen L, Martinoia E, Neuhaus HE - Lack of the Golgi phosphate transporter PHT4;6 causes strong developmental defects, constitutively activated disease resistance mechanisms and altered intracellular phosphate compartmentation in Arabidopsis
Visit the PubMed ArticleSimilarly, use of α-horseradish peroxidase antibody, which is able to bind complex-type N-glycans (especially fucose and xylose residues, Wilson et al., 1998), revealed that at least five proteins in pht4;6 leaves show altered abundance (either increased or decreased, respectively) when compared to wild-type
Hassler S, Lemke L, Jung B, Möhlmann T, Krüger F, Schumacher K, Espen L, Martinoia E, Neuhaus HE - Lack of the Golgi phosphate transporter PHT4;6 causes strong developmental defects, constitutively activated disease resistance mechanisms and altered intracellular phosphate compartmentation in Arabidopsis
Visit the PubMed ArticleWRKY75 ... In leaves of pht4;6 mutants, all four Pi-responsive genes were drastically (4.5–19-fold) up-regulated compared to wild-type plants
Hassler S, Lemke L, Jung B, Möhlmann T, Krüger F, Schumacher K, Espen L, Martinoia E, Neuhaus HE - Lack of the Golgi phosphate transporter PHT4;6 causes strong developmental defects, constitutively activated disease resistance mechanisms and altered intracellular phosphate compartmentation in Arabidopsis
Visit the PubMed Articleusing the genes coding for the ER-located ... Bip1 ... exhibit markedly increased expression levels in pht4;6 plants compared to wild-type plants
Hassler S, Lemke L, Jung B, Möhlmann T, Krüger F, Schumacher K, Espen L, Martinoia E, Neuhaus HE - Lack of the Golgi phosphate transporter PHT4;6 causes strong developmental defects, constitutively activated disease resistance mechanisms and altered intracellular phosphate compartmentation in Arabidopsis
Visit the PubMed Articleusing the genes coding for the ER-located ... calnexin1 (CNX1 ... exhibit markedly increased expression levels in pht4;6 plants compared to wild-type plants
Hassler S, Lemke L, Jung B, Möhlmann T, Krüger F, Schumacher K, Espen L, Martinoia E, Neuhaus HE - Lack of the Golgi phosphate transporter PHT4;6 causes strong developmental defects, constitutively activated disease resistance mechanisms and altered intracellular phosphate compartmentation in Arabidopsis
Visit the PubMed ArticleTo further examine the accumulation of SUB protein during leaf development, we generated transgenic plants expressing SUB–YFP under the control of a native SUB promoter (proSUB:SUB-YFP) in the sub-2 background. The SUB–YFP fluorescence signal was very weak in the leaf primordia of most transgenic plants, although the leaf phenotypes of sub-2 were rescued. We thus selected complemented plants with strong YFP signal but reduced leaf size, which resemble the phenotypes of weak SUB-OE plants (see below), for analysis. Consistent with the GUS activity of proSUB:GUS, SUB–YFP accumulation in young primordia was basipetally diminished during leaf development and became restricted to developing vascular cells (Figure 6p–r). In addition, SUB–YFP accumulated in the cell periphery of epidermal tissues (Figure 6s), similar to previous reports in root and sepal epidermal cells (Kwak and Schiefelbein, 2008; Yadav et al., 2008). Interestingly, accumulation of SUB–YFP was predominant at the junctions of two neighboring procambial cells (arrows in Figure 6t), suggesting a potential polar localization of SUB during vascular differentiation
Lin L, Zhong SH, Cui XF, Li J, He ZH - Characterization of temperature-sensitive mutants reveals a role for receptor-like kinase SCRAMBLED/STRUBBELIG in coordinating cell proliferation and differentiation during Arabidopsis leaf development
Visit the PubMed ArticleThe CYCB1;1–GUS activity decreased earlier in sub-2 plants than in Col-0 at both temperatures (Figure 3e,f and S4), consistent with the reduced cell number as shown in Figure 3(d).
Lin L, Zhong SH, Cui XF, Li J, He ZH - Characterization of temperature-sensitive mutants reveals a role for receptor-like kinase SCRAMBLED/STRUBBELIG in coordinating cell proliferation and differentiation during Arabidopsis leaf development
Visit the PubMed ArticleExpression of DR5–GUS in sub-2 leaves was slightly weaker in both tip and vascular zones but increased in the ectopic hydathodes (Figure S6a,b). Exogenous application of indole-3-acetic acid (IAA) induced DR5–GUS expression in both Col-0 and sub-2 seedlings, showing that auxin perception and response were normal in the sub-2 mutant
Lin L, Zhong SH, Cui XF, Li J, He ZH - Characterization of temperature-sensitive mutants reveals a role for receptor-like kinase SCRAMBLED/STRUBBELIG in coordinating cell proliferation and differentiation during Arabidopsis leaf development
Visit the PubMed ArticleTo examine the expression pattern of SUB during leaf development, promoter activity was analyzed using the proSUB:GUS reporter line (Figure 6a–o). The GUS signal was weak in the shoot apical meristem
Lin L, Zhong SH, Cui XF, Li J, He ZH - Characterization of temperature-sensitive mutants reveals a role for receptor-like kinase SCRAMBLED/STRUBBELIG in coordinating cell proliferation and differentiation during Arabidopsis leaf development
Visit the PubMed Articlepromoter activity was analyzed using the proSUB:GUS reporter line ... The GUS signal ... increased throughout young primordia, with higher activity in provascular tissues and the adaxial–abaxial juxtaposition region
Lin L, Zhong SH, Cui XF, Li J, He ZH - Characterization of temperature-sensitive mutants reveals a role for receptor-like kinase SCRAMBLED/STRUBBELIG in coordinating cell proliferation and differentiation during Arabidopsis leaf development
Visit the PubMed ArticleTo examine the expression pattern of SUB during leaf development, promoter activity was analyzed using the proSUB:GUS reporter line ... During leaf expansion, GUS expression was basipetally reduced and finally restricted to vascular cells that were still dividing and differentiating
Lin L, Zhong SH, Cui XF, Li J, He ZH - Characterization of temperature-sensitive mutants reveals a role for receptor-like kinase SCRAMBLED/STRUBBELIG in coordinating cell proliferation and differentiation during Arabidopsis leaf development
Visit the PubMed ArticleTo examine the early vascular development, we introduced ATHB8–GUS, a marker for procambial cells (Baima et al., 1995), into sub-2 plants. Although expression of ATHB8–GUS showed no obvious difference between sub-2 and Col-0 at leaf stage 1 (Figure 4c), the subsequent formation of continuous loops was disturbed in the sub-2 leaves
Lin L, Zhong SH, Cui XF, Li J, He ZH - Characterization of temperature-sensitive mutants reveals a role for receptor-like kinase SCRAMBLED/STRUBBELIG in coordinating cell proliferation and differentiation during Arabidopsis leaf development
Visit the PubMed ArticleExpression of the auxin efflux carrier PIN-FORMED1 (PIN1) precedes expression of ATHB8–GUS in leaf primordia, and thus represents an earlier marker for procambium formation (Scarpella et al., 2006). We used a functional PIN1–GFP reporter (Benkova et al., 2003) to examine PIN1 expression. During formation of continuous veins in wild-type leaf primordia, the PIN1–GFP expression domain was broad at first but was gradually restricted to a narrow site and became connected to the midvein PIN1–GFP expression domain or other pre-existing PIN1–GFP expression domains (Scarpella et al., 2006). In contrast, some PIN1–GFP expression domains were ectopic or even missing, or failed to extend in sub-2 plants (Figure 4f), suggesting that the auxin flow may be interrupted
Lin L, Zhong SH, Cui XF, Li J, He ZH - Characterization of temperature-sensitive mutants reveals a role for receptor-like kinase SCRAMBLED/STRUBBELIG in coordinating cell proliferation and differentiation during Arabidopsis leaf development
Visit the PubMed Articleexpression of ... AtPIN4 ... were decreased ... 35S::MIR394a
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed Articleexpression of ... AtPIN7 were decreased in ... AtPIN7 ... 35S::MIR394a
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed ArticleMIR394b ... was strongly expressed in the transgenic plants ... 35S::MIR394a/b
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed Articleexpression of LCR in 35S::MIR394a/b plants was drastically decreased
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed ArticleIAA3 ... basal level ... was slightly higher in 35S::MIR394a plants than in wild type
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed ArticleLCR expression in MIM394 ... was considerably higher
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed ArticleLCR was expressed in ... whole seedlings
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed Articleexpression of AtPIN1 ... were decreased in ... 35S::MIR394a
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed ArticleMIR394a ... cotyledons ... displayed GUS activities
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed Articleexpression of ... AtPIN7 were decreased in ... 35S::m5LCR plants
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed Articleexpression of ... AtPIN3 ... were decreased in ... 35S::m5LCR plants
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed ArticlepDR5::GUS expression ... in leaves of ... 45 days old ... 35S::m5LCR plants ... mainly occurred along the leaf margin ...
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed ArticleMIR394a ... leaves ... strong GUS staining was detected and most of GUS activities were found at conductive tissues
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed Articleexpression of ... AtPIN4 ... were decreased in ... 35S::m5LCR plants
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed ArticleMIR394b ... cotyledons ... displayed GUS activities
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed ArticlepDR5::GUS expression ... in leaves of ... 45 days old lcr ... was considerably higher than that of WT
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed ArticleAXR3 ... basal level of the gene expression ... were significantly lower in 35S::m5LCR plants
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed ArticlepDR5::GUS expression ... in leaves of ... 45 days old lcr ... mainly occurred along the leaf margin
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed Articleexpression of ... AtPIN3 ... were decreased in ... 35S::MIR394a
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed Articlethere was no change in LCR expression in response to IAA exposure
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed ArticleIAMT1 ... basal level ... was slightly higher in 35S::MIR394a plants than in wild type
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed ArticleIn wild type ... leaves ... the pDR5::GUS expression ... was restricted to the ... hydathodes
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed ArticleLCR transcript levels ... in lcr mutant was 17% of that in the wild type
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed ArticleIAMT1 ... basal level of the gene expression ... were significantly lower in 35S::m5LCR plants
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed ArticlemiR394b ... accumulates in ... whole seedlings
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed ArticleLCR transcript ... in 35S::MIR394a/b plants was drastically decreased
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed ArticleLCR was expressed in ... leaves
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed ArticlemiR394a ... accumulates in ... whole seedlings
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed ArticleMIR394b ... leaves ... strong GUS staining was detected and most of GUS activities were found at conductive tissues
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed ArticlepDR5::GUS expression ... in leaves of ... 45 days old ... 35S::m5LCR plants was considerably higher than that of WT
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed ArticleAXR3 ... basal level ... was slightly higher in 35S::MIR394a plants than in wild type
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed ArticleMIR394b ... at very early stage (four days) of leaf development, no GUS staining was detected
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed Articlein ... pLCR::m5LCR ... LCR expression was 6.4-7.7 folds higher than in the wild type
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed Articlein MIM394 transgenic lines ... the abundance of LCR transcripts ... was considerably higher
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed ArticleMIR394a ... was strongly expressed in the transgenic plants ... 35S::MIR394a/b
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed ArticleMIR394a ... at very early stage (four days) of leaf development, no GUS staining was detected
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed ArticlemiR394a ... accumulates in ... leaves
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed Articleexpression of AtPIN1 ... were decreased in ... 35S::m5LCR plants
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed ArticlemiR394b ... accumulates in ... leaves
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed Articlethat the level of LCR mRNA ... in 35S::LCR plants was increased only by 1.7-2.5 folds compared with ... WT
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed ArticleIAA3 ... basal level of the gene expression ... were significantly lower in 35S::m5LCR plants
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed Articlethe level of LCR mRNA in 35S::m5LCR plants was 34.4-40.5 folds higher than those of WT
Song JB, Huang SQ, Dalmay T, Yang ZM - Regulation of Leaf Morphology by MicroRNA394 and Its Target LEAF CURLING RESPONSIVENESS
Visit the PubMed Articleanalyzed the expression profile of a set of 28 cell cycle marker and growth-related genes (Table S3). WT and samba plants were grown in vitro for 8, 10, and 12 DAS, and RNA levels in dissected leaves 1 and 2 were measured with the nCounter system that allows a direct multiplexed analysis of selected transcripts (37). Most of the G2/M-phase–related genes analyzed, such as CYCB1;1, cyclin-dependent kinase CDKB2;1, and 3xHMG-box2 (38⇓–40), were significantly down-regulated at all three developmental time points in samba mutants, whereas genes encoding cell cycle inhibitors, such as KIP-RELATED PROTEIN 1, SIAMESE RELATED 1 (SMR1), SMR2, and SMR3, were up-regulated, in particular at 8 DAS
Eloy NB, Gonzalez N, Van Leene J, Maleux K, Vanhaeren H, De Milde L, Dhondt S, Vercruysse L, Witters E, Mercier R, Cromer L, Beemster GT, Remaut H, Van Montagu MC, De Jaeger G, Ferreira PC, Inzé D - SAMBA, a plant-specific anaphase-promoting complex/cyclosome regulator is involved in early development and A-type cyclin stabilization
Visit the PubMed Articleanalyzed the expression profile of a set of 28 cell cycle marker and growth-related genes (Table S3). WT and samba plants were grown in vitro for 8, 10, and 12 DAS, and RNA levels in dissected leaves 1 and 2 were measured with the nCounter system that allows a direct multiplexed analysis of selected transcripts (37 ... A similar expression profile was found for the SHOOT MERISTEMLESS and EXPANSIN11 (EXP11
Eloy NB, Gonzalez N, Van Leene J, Maleux K, Vanhaeren H, De Milde L, Dhondt S, Vercruysse L, Witters E, Mercier R, Cromer L, Beemster GT, Remaut H, Van Montagu MC, De Jaeger G, Ferreira PC, Inzé D - SAMBA, a plant-specific anaphase-promoting complex/cyclosome regulator is involved in early development and A-type cyclin stabilization
Visit the PubMed Articleanalyzed the expression profile of a set of 28 cell cycle marker and growth-related genes (Table S3). WT and samba plants were grown in vitro for 8, 10, and 12 DAS, and RNA levels in dissected leaves 1 and 2 were measured with the nCounter system that allows a direct multiplexed analysis of selected transcripts (37). Most of the G2/M-phase–related genes analyzed, such as CYCB1;1, cyclin-dependent kinase CDKB2;1, and 3xHMG-box2 (38⇓–40), were significantly down-regulated at all three developmental time points in samba mutants, whereas genes encoding cell cycle inhibitors, such as KIP-RELATED PROTEIN 1, SIAMESE RELATED 1 (SMR1), SMR2, and SMR3, were up-regulated, in particular at 8 DAS
Eloy NB, Gonzalez N, Van Leene J, Maleux K, Vanhaeren H, De Milde L, Dhondt S, Vercruysse L, Witters E, Mercier R, Cromer L, Beemster GT, Remaut H, Van Montagu MC, De Jaeger G, Ferreira PC, Inzé D - SAMBA, a plant-specific anaphase-promoting complex/cyclosome regulator is involved in early development and A-type cyclin stabilization
Visit the PubMed ArticleTo study the expression pattern of SAMBA, a 1.8-kb fragment upstream of the ATG codon of the SAMBA gene was fused to a β-glucuronidase (GUS)-GFP tandem reporter cassette and introduced into Arabidopsis plants. SAMBA expression was high during embryogenesis (Fig. 1 B and C) but decreased gradually when seedlings germinated (Fig. 1 D and F
Eloy NB, Gonzalez N, Van Leene J, Maleux K, Vanhaeren H, De Milde L, Dhondt S, Vercruysse L, Witters E, Mercier R, Cromer L, Beemster GT, Remaut H, Van Montagu MC, De Jaeger G, Ferreira PC, Inzé D - SAMBA, a plant-specific anaphase-promoting complex/cyclosome regulator is involved in early development and A-type cyclin stabilization
Visit the PubMed Articleanalyzed the expression profile of a set of 28 cell cycle marker and growth-related genes (Table S3). WT and samba plants were grown in vitro for 8, 10, and 12 DAS, and RNA levels in dissected leaves 1 and 2 were measured with the nCounter system that allows a direct multiplexed analysis of selected transcripts (37). Most of the G2/M-phase–related genes analyzed, such as CYCB1;1, cyclin-dependent kinase CDKB2;1, and 3xHMG-box2 (38⇓–40), were significantly down-regulated at all three developmental time points in samba mutants, whereas genes encoding cell cycle inhibitors, such as KIP-RELATED PROTEIN 1, SIAMESE RELATED 1 (SMR1), SMR2, and SMR3, were up-regulated, in particular at 8 DAS
Eloy NB, Gonzalez N, Van Leene J, Maleux K, Vanhaeren H, De Milde L, Dhondt S, Vercruysse L, Witters E, Mercier R, Cromer L, Beemster GT, Remaut H, Van Montagu MC, De Jaeger G, Ferreira PC, Inzé D - SAMBA, a plant-specific anaphase-promoting complex/cyclosome regulator is involved in early development and A-type cyclin stabilization
Visit the PubMed ArticleTo study the expression pattern of SAMBA, a 1.8-kb fragment upstream of the ATG codon of the SAMBA gene was fused to a β-glucuronidase (GUS)-GFP tandem reporter cassette and introduced into Arabidopsis plants. SAMBA expression was high during embryogenesis (Fig. 1 B and C) but decreased gradually when seedlings germinated (Fig. 1 D and F). At 3 d after stratification (DAS) (Fig. 1D), GUS staining was still well visible in all tissues;
Eloy NB, Gonzalez N, Van Leene J, Maleux K, Vanhaeren H, De Milde L, Dhondt S, Vercruysse L, Witters E, Mercier R, Cromer L, Beemster GT, Remaut H, Van Montagu MC, De Jaeger G, Ferreira PC, Inzé D - SAMBA, a plant-specific anaphase-promoting complex/cyclosome regulator is involved in early development and A-type cyclin stabilization
Visit the PubMed ArticleTo study the expression pattern of SAMBA, a 1.8-kb fragment upstream of the ATG codon of the SAMBA gene was fused to a β-glucuronidase (GUS)-GFP tandem reporter cassette and introduced into Arabidopsis plants. SAMBA expression was high during embryogenesis (Fig. 1 B and C) but decreased gradually when seedlings germinated (Fig. 1 D and F). At 3 d after stratification (DAS) (Fig. 1D), GUS staining was still well visible in all tissues; at 5 DAS, it diminished and became patchy in root tissues (Fig. 1E); finally, at 8 DAS, it was present only in the hypocotyls
Eloy NB, Gonzalez N, Van Leene J, Maleux K, Vanhaeren H, De Milde L, Dhondt S, Vercruysse L, Witters E, Mercier R, Cromer L, Beemster GT, Remaut H, Van Montagu MC, De Jaeger G, Ferreira PC, Inzé D - SAMBA, a plant-specific anaphase-promoting complex/cyclosome regulator is involved in early development and A-type cyclin stabilization
Visit the PubMed ArticleWT and samba plants were grown in vitro for 8, 10, and 12 DAS, and RNA levels in dissected leaves 1 and 2 were measured with the nCounter system that allows a direct multiplexed analysis of selected transcripts (37). Most of the G2/M-phase–related genes analyzed, such as CYCB1;1, cyclin-dependent kinase CDKB2;1, and 3xHMG-box2 (38⇓–40), were significantly down-regulated at all three developmental time points in samba mutants, whereas genes encoding cell cycle inhibitors, such as KIP-RELATED PROTEIN 1, SIAMESE RELATED 1 (SMR1), SMR2, and SMR3, were up-regulated, in particular at 8 DAS
Eloy NB, Gonzalez N, Van Leene J, Maleux K, Vanhaeren H, De Milde L, Dhondt S, Vercruysse L, Witters E, Mercier R, Cromer L, Beemster GT, Remaut H, Van Montagu MC, De Jaeger G, Ferreira PC, Inzé D - SAMBA, a plant-specific anaphase-promoting complex/cyclosome regulator is involved in early development and A-type cyclin stabilization
Visit the PubMed ArticleExpression of the pCYCB1;1:CYCB1;1 D-box-GUS/GFP construct allowed us to estimate the rate of CYCB1;1 degradation in proliferating cells (28, 41). The CYCB1;1 degradation rate did not differ in the leaf primordia or in the RAM of WT and samba mutant plants (Fig. S4 D and E), suggesting that SAMBA specifically targets A2-type and not B-type cyclins for degradation
Eloy NB, Gonzalez N, Van Leene J, Maleux K, Vanhaeren H, De Milde L, Dhondt S, Vercruysse L, Witters E, Mercier R, Cromer L, Beemster GT, Remaut H, Van Montagu MC, De Jaeger G, Ferreira PC, Inzé D - SAMBA, a plant-specific anaphase-promoting complex/cyclosome regulator is involved in early development and A-type cyclin stabilization
Visit the PubMed Articleanalyzed the expression profile of a set of 28 cell cycle marker and growth-related genes (Table S3). WT and samba plants were grown in vitro for 8, 10, and 12 DAS, and RNA levels in dissected leaves 1 and 2 were measured with the nCounter system that allows a direct multiplexed analysis of selected transcripts (37 ... A similar expression profile was found for the SHOOT MERISTEMLESS and EXPANSIN11 (EXP11
Eloy NB, Gonzalez N, Van Leene J, Maleux K, Vanhaeren H, De Milde L, Dhondt S, Vercruysse L, Witters E, Mercier R, Cromer L, Beemster GT, Remaut H, Van Montagu MC, De Jaeger G, Ferreira PC, Inzé D - SAMBA, a plant-specific anaphase-promoting complex/cyclosome regulator is involved in early development and A-type cyclin stabilization
Visit the PubMed ArticleTo study the expression pattern of SAMBA, a 1.8-kb fragment upstream of the ATG codon of the SAMBA gene was fused to a β-glucuronidase (GUS)-GFP tandem reporter cassette and introduced into Arabidopsis plants. SAMBA expression was high during embryogenesis (Fig. 1 B and C) but decreased gradually when seedlings germinated (Fig. 1 D and F). At 3 d after stratification (DAS) (Fig. 1D), GUS staining was still well visible in all tissues; at 5 DAS, it diminished and became patchy in root tissues
Eloy NB, Gonzalez N, Van Leene J, Maleux K, Vanhaeren H, De Milde L, Dhondt S, Vercruysse L, Witters E, Mercier R, Cromer L, Beemster GT, Remaut H, Van Montagu MC, De Jaeger G, Ferreira PC, Inzé D - SAMBA, a plant-specific anaphase-promoting complex/cyclosome regulator is involved in early development and A-type cyclin stabilization
Visit the PubMed ArticleTo study the expression pattern of SAMBA, a 1.8-kb fragment upstream of the ATG codon of the SAMBA gene was fused to a β-glucuronidase (GUS)-GFP tandem reporter cassette and introduced into Arabidopsis plants. SAMBA expression was high during embryogenesis (Fig. 1 B and C) but decreased gradually when seedlings germinated (Fig. 1 D and F
Eloy NB, Gonzalez N, Van Leene J, Maleux K, Vanhaeren H, De Milde L, Dhondt S, Vercruysse L, Witters E, Mercier R, Cromer L, Beemster GT, Remaut H, Van Montagu MC, De Jaeger G, Ferreira PC, Inzé D - SAMBA, a plant-specific anaphase-promoting complex/cyclosome regulator is involved in early development and A-type cyclin stabilization
Visit the PubMed ArticleWT and samba plants were grown in vitro for 8, 10, and 12 DAS, and RNA levels in dissected leaves 1 and 2 were measured with the nCounter system that allows a direct multiplexed analysis of selected transcripts (37). Most of the G2/M-phase–related genes analyzed, such as CYCB1;1, cyclin-dependent kinase CDKB2;1, and 3xHMG-box2 (38⇓–40), were significantly down-regulated at all three developmental time points in samba mutants, whereas genes encoding cell cycle inhibitors, such as KIP-RELATED PROTEIN 1, SIAMESE RELATED 1 (SMR1), SMR2, and SMR3, were up-regulated, in particular at 8 DAS
Eloy NB, Gonzalez N, Van Leene J, Maleux K, Vanhaeren H, De Milde L, Dhondt S, Vercruysse L, Witters E, Mercier R, Cromer L, Beemster GT, Remaut H, Van Montagu MC, De Jaeger G, Ferreira PC, Inzé D - SAMBA, a plant-specific anaphase-promoting complex/cyclosome regulator is involved in early development and A-type cyclin stabilization
Visit the PubMed ArticleTo study the expression pattern of SAMBA, a 1.8-kb fragment upstream of the ATG codon of the SAMBA gene was fused to a β-glucuronidase (GUS)-GFP tandem reporter cassette and introduced into Arabidopsis plants. SAMBA expression was high during embryogenesis (Fig. 1 B and C) but decreased gradually when seedlings germinated (Fig. 1 D and F). At 3 d after stratification (DAS) (Fig. 1D), GUS staining was still well visible in all tissues; at 5 DAS, it diminished and became patchy in root tissues (Fig. 1E); finally, at 8 DAS, it was present only in the hypocotyls (Fig. 1F); and, at later stages of development, the expression was only observed in pollen grains
Eloy NB, Gonzalez N, Van Leene J, Maleux K, Vanhaeren H, De Milde L, Dhondt S, Vercruysse L, Witters E, Mercier R, Cromer L, Beemster GT, Remaut H, Van Montagu MC, De Jaeger G, Ferreira PC, Inzé D - SAMBA, a plant-specific anaphase-promoting complex/cyclosome regulator is involved in early development and A-type cyclin stabilization
Visit the PubMed ArticleWT and samba plants were grown in vitro for 8, 10, and 12 DAS, and RNA levels in dissected leaves 1 and 2 were measured with the nCounter system that allows a direct multiplexed analysis of selected transcripts (37). Most of the G2/M-phase–related genes analyzed, such as CYCB1;1, cyclin-dependent kinase CDKB2;1, and 3xHMG-box2 (38⇓–40), were significantly down-regulated at all three developmental time points in samba mutants, whereas genes encoding cell cycle inhibitors, such as KIP-RELATED PROTEIN 1, SIAMESE RELATED 1 (SMR1), SMR2, and SMR3, were up-regulated, in particular at 8 DAS
Eloy NB, Gonzalez N, Van Leene J, Maleux K, Vanhaeren H, De Milde L, Dhondt S, Vercruysse L, Witters E, Mercier R, Cromer L, Beemster GT, Remaut H, Van Montagu MC, De Jaeger G, Ferreira PC, Inzé D - SAMBA, a plant-specific anaphase-promoting complex/cyclosome regulator is involved in early development and A-type cyclin stabilization
Visit the PubMed ArticleqRT-PCR results using dissected SAM from 10-day-old seedlings indicated that ... CLV3 maintained slightly higher mRNA expression levels in ccs52a2-3 mutants when compared with WT
Liu Y, Ye W, Li B, Zhou X, Cui Y, Running MP, Liu K - CCS52A2/FZR1, a cell cycle regulator, is an essential factor for shoot apical meristem maintenance in Arabidopsis thaliana
Visit the PubMed ArticleTo determine the expression region of CCS52A2 mRNA, in situ hybridization was carried out ... strong signals in the ... lateral leaf primordia in 8-day-old seedlings
Liu Y, Ye W, Li B, Zhou X, Cui Y, Running MP, Liu K - CCS52A2/FZR1, a cell cycle regulator, is an essential factor for shoot apical meristem maintenance in Arabidopsis thaliana
Visit the PubMed ArticleSince CCS52A2 acts at G2/M to control mitosis exit and endocycle entry, we checked the expression of the G2/M transition reporter gene CYCB 1;1 in ccs52a2-3 mutants ... qRT PCR was carried out to quantify the expression levels of CYCB1;1 in the shoot. The seedling SAM of ccs52a2-3 showed lower expression level in comparison with that of WT
Liu Y, Ye W, Li B, Zhou X, Cui Y, Running MP, Liu K - CCS52A2/FZR1, a cell cycle regulator, is an essential factor for shoot apical meristem maintenance in Arabidopsis thaliana
Visit the PubMed Articlethe pCLV3::GUS location in ccs52a2-3 SAM exhibited two nearby domains of CLV3 expression in 6 of 22 8-day–old seedlings examined (Figure 6M), which indicated that two separate WUS-CLV3 supporting systems of meristem organization had been established as early as the 8-day-old stage. It was also discovered that CLV3 showed broad expression in some first layer cells that flank the major expression zone in 7 of 22 SAMs of ccs52a2-3 seedlings (Figure 6N), while the remaining 9 of 22 ccs52a2-3 SAMs exhibited a single CLV3 expression region
Liu Y, Ye W, Li B, Zhou X, Cui Y, Running MP, Liu K - CCS52A2/FZR1, a cell cycle regulator, is an essential factor for shoot apical meristem maintenance in Arabidopsis thaliana
Visit the PubMed ArticleRT-PCR analysis indicated that CCS52A2 had a broad expression profile including roots, rosette leaves, stem, cauline leaves, inflorescences and siliques
Liu Y, Ye W, Li B, Zhou X, Cui Y, Running MP, Liu K - CCS52A2/FZR1, a cell cycle regulator, is an essential factor for shoot apical meristem maintenance in Arabidopsis thaliana
Visit the PubMed ArticleThe expression of CCS52A2 was also examined in 8-day-old plants by a histochemical GUS assays, using transgenic plants expressing the CCS52A2::GUS fusion protein under the control of the CCS52A2 native promoter. Strong expression was observed in shoot apices
Liu Y, Ye W, Li B, Zhou X, Cui Y, Running MP, Liu K - CCS52A2/FZR1, a cell cycle regulator, is an essential factor for shoot apical meristem maintenance in Arabidopsis thaliana
Visit the PubMed ArticleThe expression of WUS in WT is restricted in a small region composed of a few cells in the center of the SAM (Figure 6A, D) [4]. However, in the SAM of 8-day-old ccs52a2-3 seedlings, WUS expressed in one enlarged domain at higher levels (Figure 6B), or in two distinct nearby domains (Figure 6C), suggesting that enlarged or multiple OC zones are formed, which is consistent with the broadening SAMs and bifurcations of the stem
Liu Y, Ye W, Li B, Zhou X, Cui Y, Running MP, Liu K - CCS52A2/FZR1, a cell cycle regulator, is an essential factor for shoot apical meristem maintenance in Arabidopsis thaliana
Visit the PubMed ArticleThe pWUS::GUS assay presented a similar expression pattern as RNA in situs: enlarged and sometimes dual expression domains in the ccs52a2-3 background
Liu Y, Ye W, Li B, Zhou X, Cui Y, Running MP, Liu K - CCS52A2/FZR1, a cell cycle regulator, is an essential factor for shoot apical meristem maintenance in Arabidopsis thaliana
Visit the PubMed ArticleTo determine the expression region of CCS52A2 mRNA, in situ hybridization was carried out. The results were consistent with that of the GUS assay, presenting strong signals in the entire SAM
Liu Y, Ye W, Li B, Zhou X, Cui Y, Running MP, Liu K - CCS52A2/FZR1, a cell cycle regulator, is an essential factor for shoot apical meristem maintenance in Arabidopsis thaliana
Visit the PubMed ArticleIn WT, CLV3 mRNA accumulates in a small zone of cells in the first three layers at the meristem apex (Figure 6G, I) [6]. Compared with WT, the SAMs of 8-day-old ccs52a2-3 seedlings that were examined exhibited increased CLV3 expression
Liu Y, Ye W, Li B, Zhou X, Cui Y, Running MP, Liu K - CCS52A2/FZR1, a cell cycle regulator, is an essential factor for shoot apical meristem maintenance in Arabidopsis thaliana
Visit the PubMed ArticleqRT-PCR results using dissected SAM from 10-day-old seedlings indicated that WUS was expressed at a higher level in ccs52a2-3
Liu Y, Ye W, Li B, Zhou X, Cui Y, Running MP, Liu K - CCS52A2/FZR1, a cell cycle regulator, is an essential factor for shoot apical meristem maintenance in Arabidopsis thaliana
Visit the PubMed Articlein mrn1, the expression levels of the ... EXP10 ... not altered when compared with the wild type
Go YS, Lee SB, Kim HJ, Kim J, Park HY, Kim JK, Shibata K, Yokota T, Ohyama K, Muranaka T, Arseniyadis S, Suh MC - Identification of marneral synthase, which is critical for growth and development in Arabidopsis
Visit the PubMed ArticleMRN1 gene, which is specifically expressed in roots and seedlings
Go YS, Lee SB, Kim HJ, Kim J, Park HY, Kim JK, Shibata K, Yokota T, Ohyama K, Muranaka T, Arseniyadis S, Suh MC - Identification of marneral synthase, which is critical for growth and development in Arabidopsis
Visit the PubMed Articlein mrn1, the expression levels of the EXP5 ... not altered when compared with the wild type
Go YS, Lee SB, Kim HJ, Kim J, Park HY, Kim JK, Shibata K, Yokota T, Ohyama K, Muranaka T, Arseniyadis S, Suh MC - Identification of marneral synthase, which is critical for growth and development in Arabidopsis
Visit the PubMed ArticleTo investigate the subcellular localization of MRN1, the plasmid 35Spro:eGFP:MRN1 encoding enhanced green fluorescence protein (eGFP) fused to N-terminal region of MRN1 under the control of the CaMV35S promoter was introduced into tobacco leaf protoplasts, and the subcellular localization of eGFP:MRN1 fusion proteins was analyzed by confocal laser scanning microscopy. The green fluorescent signals from the eGFP:MRN1 were merged with the red fluorescent signals from the ER marker, Brassica rapa fatty acid desaturase 2:RFP (BrFAD2:RFP, Jung et al., 2011) (Figure 10a–c), suggesting that MRN1 is localized in the ER
Go YS, Lee SB, Kim HJ, Kim J, Park HY, Kim JK, Shibata K, Yokota T, Ohyama K, Muranaka T, Arseniyadis S, Suh MC - Identification of marneral synthase, which is critical for growth and development in Arabidopsis
Visit the PubMed ArticleThe expression pattern of the MRN1 gene, which is specifically expressed in roots and seedlings, was similar to that of THAS/PEN4
Go YS, Lee SB, Kim HJ, Kim J, Park HY, Kim JK, Shibata K, Yokota T, Ohyama K, Muranaka T, Arseniyadis S, Suh MC - Identification of marneral synthase, which is critical for growth and development in Arabidopsis
Visit the PubMed ArticleThe expression pattern of the MRN1 gene, which is specifically expressed in roots and seedlings, was similar to that of ... PEN1
Go YS, Lee SB, Kim HJ, Kim J, Park HY, Kim JK, Shibata K, Yokota T, Ohyama K, Muranaka T, Arseniyadis S, Suh MC - Identification of marneral synthase, which is critical for growth and development in Arabidopsis
Visit the PubMed ArticleA RT-PCR analysis of the transcripts of the genes related to flowering time control in the wild type and mrn1 showed that the transcript levels of various flowering time genes were affected (Figure S1). Among them, the transcript levels of the FLC (Flowering Locus C) gene encoding a repressor of flowering were highly elevated, indicating that the late-flowering phenotype of mrn1 might be in part due to overexpression of FLC
Go YS, Lee SB, Kim HJ, Kim J, Park HY, Kim JK, Shibata K, Yokota T, Ohyama K, Muranaka T, Arseniyadis S, Suh MC - Identification of marneral synthase, which is critical for growth and development in Arabidopsis
Visit the PubMed Articlein mrn1, the expression levels of the ... EXP6 ... not altered when compared with the wild type
Go YS, Lee SB, Kim HJ, Kim J, Park HY, Kim JK, Shibata K, Yokota T, Ohyama K, Muranaka T, Arseniyadis S, Suh MC - Identification of marneral synthase, which is critical for growth and development in Arabidopsis
Visit the PubMed Articlethe expression of genes involved in sterol and brassinosteroid biosynthesis was not altered in the mrn1
Go YS, Lee SB, Kim HJ, Kim J, Park HY, Kim JK, Shibata K, Yokota T, Ohyama K, Muranaka T, Arseniyadis S, Suh MC - Identification of marneral synthase, which is critical for growth and development in Arabidopsis
Visit the PubMed Articleubiquitous expression of CAS1
Go YS, Lee SB, Kim HJ, Kim J, Park HY, Kim JK, Shibata K, Yokota T, Ohyama K, Muranaka T, Arseniyadis S, Suh MC - Identification of marneral synthase, which is critical for growth and development in Arabidopsis
Visit the PubMed ArticleExpression of the PEN2 transcripts was observed in roots, young seedlings, and siliques
Go YS, Lee SB, Kim HJ, Kim J, Park HY, Kim JK, Shibata K, Yokota T, Ohyama K, Muranaka T, Arseniyadis S, Suh MC - Identification of marneral synthase, which is critical for growth and development in Arabidopsis
Visit the PubMed ArticleTHAS, which belongs to Arabidopsis clade II OSCs, including MRN1, was fused to eGFP and introduced into tobacco protoplasts. The green fluorescent signals from eGFP:THAS were also observed in the ER
Go YS, Lee SB, Kim HJ, Kim J, Park HY, Kim JK, Shibata K, Yokota T, Ohyama K, Muranaka T, Arseniyadis S, Suh MC - Identification of marneral synthase, which is critical for growth and development in Arabidopsis
Visit the PubMed ArticleTo investigate the temporal and spatial expression patterns of the MRN1 gene, transgenic Arabidopsis harboring approximately 5.4 kb of the promoter region fused to a GUS reporter gene was generated. Expression of GUS was exclusively detected in the meristematic zones of shoots and roots
Go YS, Lee SB, Kim HJ, Kim J, Park HY, Kim JK, Shibata K, Yokota T, Ohyama K, Muranaka T, Arseniyadis S, Suh MC - Identification of marneral synthase, which is critical for growth and development in Arabidopsis
Visit the PubMed ArticleThe expression pattern of the MRN1 gene, which is specifically expressed in roots and seedlings, was similar to that of ... pentacyclic triterpene synthase 3 (PEN3
Go YS, Lee SB, Kim HJ, Kim J, Park HY, Kim JK, Shibata K, Yokota T, Ohyama K, Muranaka T, Arseniyadis S, Suh MC - Identification of marneral synthase, which is critical for growth and development in Arabidopsis
Visit the PubMed Articlesemi-quantitative RT-PCR was performed ... AtCSP3 transcript was not detected in atcsp3-1
Yang Y, Karlson D - Effects of mutations in the Arabidopsis Cold Shock Domain Protein 3 (AtCSP3) gene on leaf cell expansion
Visit the PubMed ArticleROT3 ... was not altered relative to the wild type ... in atcsp3 mutants
Yang Y, Karlson D - Effects of mutations in the Arabidopsis Cold Shock Domain Protein 3 (AtCSP3) gene on leaf cell expansion
Visit the PubMed ArticleAN ... not altered in atcsp3 mutants
Yang Y, Karlson D - Effects of mutations in the Arabidopsis Cold Shock Domain Protein 3 (AtCSP3) gene on leaf cell expansion
Visit the PubMed Articlethe qRT-PCR results indicated that ... AtCSP3 ... elevated expression in meristematic ... tissues
Yang Y, Karlson D - Effects of mutations in the Arabidopsis Cold Shock Domain Protein 3 (AtCSP3) gene on leaf cell expansion
Visit the PubMed ArticleAN3 ... not altered in atcsp3 mutants
Yang Y, Karlson D - Effects of mutations in the Arabidopsis Cold Shock Domain Protein 3 (AtCSP3) gene on leaf cell expansion
Visit the PubMed Articletranscript levels of LNG1 were clearly reduced in atcsp3-2 ... at 28 DAG
Yang Y, Karlson D - Effects of mutations in the Arabidopsis Cold Shock Domain Protein 3 (AtCSP3) gene on leaf cell expansion
Visit the PubMed ArticleTo determine the subcellular localization of the AtCSP3 full-length protein, its complete coding sequence was fused to the N-terminus of sGFP(S65T) which was driven by a CaMV 35S promoter (Fig. 7A). The sGFP(S65T) and 35S:AtCSP3:sGFP plasmids were bombarded into onion epidermal cells with a particle bombardment delivery system and cells that were transiently expressing GFP were visualized with confocal microscopy ... transiently expressed 35S:AtCSP3:sGFP fusion proteins fluoresced in ... the cytosol
Yang Y, Karlson D - Effects of mutations in the Arabidopsis Cold Shock Domain Protein 3 (AtCSP3) gene on leaf cell expansion
Visit the PubMed ArticleLNG2 gene expression in ... atcsp3-1 did not show the same pattern of gene expression as LNG1
Yang Y, Karlson D - Effects of mutations in the Arabidopsis Cold Shock Domain Protein 3 (AtCSP3) gene on leaf cell expansion
Visit the PubMed ArticleLNG2 gene expression in atcsp3-2 ... did not show the same pattern of gene expression as LNG1
Yang Y, Karlson D - Effects of mutations in the Arabidopsis Cold Shock Domain Protein 3 (AtCSP3) gene on leaf cell expansion
Visit the PubMed ArticleTo determine the subcellular localization of the AtCSP3 full-length protein, its complete coding sequence was fused to the N-terminus of sGFP(S65T) which was driven by a CaMV 35S promoter (Fig. 7A). The sGFP(S65T) and 35S:AtCSP3:sGFP plasmids were bombarded into onion epidermal cells with a particle bombardment delivery system and cells that were transiently expressing GFP were visualized with confocal microscopy ... transiently expressed 35S:AtCSP3:sGFP fusion proteins fluoresced in ... the ... nucleus
Yang Y, Karlson D - Effects of mutations in the Arabidopsis Cold Shock Domain Protein 3 (AtCSP3) gene on leaf cell expansion
Visit the PubMed Articlesemi-quantitative RT-PCR was performed ... AtCSP3 transcript was not detected in ... atcsp3-2
Yang Y, Karlson D - Effects of mutations in the Arabidopsis Cold Shock Domain Protein 3 (AtCSP3) gene on leaf cell expansion
Visit the PubMed Articletranscript levels of LNG1 were clearly reduced in ... atcsp3-1 at 28 DAG
Yang Y, Karlson D - Effects of mutations in the Arabidopsis Cold Shock Domain Protein 3 (AtCSP3) gene on leaf cell expansion
Visit the PubMed ArticleCYCD3;1 ... not altered in atcsp3 mutants
Yang Y, Karlson D - Effects of mutations in the Arabidopsis Cold Shock Domain Protein 3 (AtCSP3) gene on leaf cell expansion
Visit the PubMed ArticleQuantitative real-time PCR (qRT-PCR) confirmed the expression of AtCSP3 transcripts in all vegetative and reproductive tissues
Yang Y, Karlson D - Effects of mutations in the Arabidopsis Cold Shock Domain Protein 3 (AtCSP3) gene on leaf cell expansion
Visit the PubMed ArticleCESA4 ... up-regulated more than sixfold by constitutive or inducible over-expression of MYB46 ... The expression patterns of the CESA genes were correlated with MYB46 expression levels
Kim WC, Ko JH, Kim JY, Kim JM, Bae HJ, Han KH - MYB46 directly regulates the gene expression of secondary wall-associated cellulose synthases in Arabidopsis
Visit the PubMed ArticleCESA8 ... co-expressed with MYB46
Kim WC, Ko JH, Kim JY, Kim JM, Bae HJ, Han KH - MYB46 directly regulates the gene expression of secondary wall-associated cellulose synthases in Arabidopsis
Visit the PubMed ArticleCESA4 ... co-expressed with MYB46
Kim WC, Ko JH, Kim JY, Kim JM, Bae HJ, Han KH - MYB46 directly regulates the gene expression of secondary wall-associated cellulose synthases in Arabidopsis
Visit the PubMed ArticleCESA7 ... up-regulated more than sixfold by constitutive or inducible over-expression of MYB46 ... The expression patterns of the CESA genes were correlated with MYB46 expression levels
Kim WC, Ko JH, Kim JY, Kim JM, Bae HJ, Han KH - MYB46 directly regulates the gene expression of secondary wall-associated cellulose synthases in Arabidopsis
Visit the PubMed ArticleCESA8 ... up-regulated more than sixfold by constitutive or inducible over-expression of MYB46 ... The expression patterns of the CESA genes were correlated with MYB46 expression levels
Kim WC, Ko JH, Kim JY, Kim JM, Bae HJ, Han KH - MYB46 directly regulates the gene expression of secondary wall-associated cellulose synthases in Arabidopsis
Visit the PubMed ArticleCESA7 ... co-expressed with MYB46
Kim WC, Ko JH, Kim JY, Kim JM, Bae HJ, Han KH - MYB46 directly regulates the gene expression of secondary wall-associated cellulose synthases in Arabidopsis
Visit the PubMed ArticleOther plastid-encoded subunits of thylakoid multiprotein complexes, including ... the large subunit of Rubisco (RbcL) also showed marked declines in level in mutant plants, particularly in prpl24-1 homozygotes
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed Articlein ... prps1-1 ... Other plastid-encoded subunits of thylakoid multiprotein complexes, including the reaction centre of PSI (PsaA ... also showed marked declines in level
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed Articlein ... prps17-1 ... Other plastid-encoded subunits of thylakoid multiprotein complexes, including the reaction centre of PSI ... Psa ... B ... also showed marked declines in level
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed ArticleQuantification of banding patterns on Coomassie-stained 2D BN/SDS polyacrylamide (PA) gels revealed marked reductions in levels of the plastid-encoded PSII core subunits ... PsbD in prpl24-1
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed ArticleQuantification of banding patterns on Coomassie-stained 2D BN/SDS polyacrylamide (PA) gels revealed marked reductions in levels of the plastid-encoded PSII core subunits ... PsbB ... in ... prps1-1
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed Articleprps17-1 ... increase was also observed for ... transcripts of the psaA–psaB and atpB–atpE operons, monitored by employing psaB- and atpB-specific probes
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed Articlein ... prps17-1 ... decrease in amounts of nuclear-encoded PSI subunits, such as ... PsaO
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed ArticleQuantification of banding patterns on Coomassie-stained 2D BN/SDS polyacrylamide (PA) gels revealed marked reductions in levels of the plastid-encoded PSII core subunits ... PsbD in ... prps17-1
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed Articlein ... prps1-1 ... Other plastid-encoded subunits of thylakoid multiprotein complexes, including the ... large subunit of Rubisco (RbcL) also showed marked declines in level
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed Articleprps1-1 ... increase was also observed for ... transcripts of the psaA
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed Articleonly one transcript variant was detectable for ... PRPL24 (PRPL24.1) in both leaves and siliques
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed Articlein ... prps1-1 ... Other plastid-encoded subunits of thylakoid multiprotein complexes, including ... the β-subunit of ATPase (ATPase β) ... also showed marked declines in level
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed ArticleQuantification of banding patterns on Coomassie-stained 2D BN/SDS polyacrylamide (PA) gels revealed marked reductions in levels of the plastid-encoded PSII core subunits PsbA ... in ... prps17-1
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed ArticleQuantification of banding patterns on Coomassie-stained 2D BN/SDS polyacrylamide (PA) gels revealed marked reductions in levels of the plastid-encoded PSII core subunits ... PsbC ... in ... prps17-1
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed ArticleQuantification of banding patterns on Coomassie-stained 2D BN/SDS polyacrylamide (PA) gels revealed marked reductions in levels of the plastid-encoded PSII core subunits ... PsbC ... in prpl24-1
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed Articlein ... prps1-1 ... decrease in amounts of nuclear-encoded PSI subunits, such as ... PsaO
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed Articlein ... prps1-1 ... Other plastid-encoded subunits of thylakoid multiprotein complexes, including the reaction centre of PSI ... Psa ... B ... also showed marked declines in level
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed Articlein ... prps17-1 ... Other plastid-encoded subunits of thylakoid multiprotein complexes, including the ... PsaA ... also showed marked declines in level
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed Articlein ... prps1-1 ... decrease in amounts of nuclear-encoded PSI subunits, such as ... PsaF
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed Articlein ... prps17-1 ... decrease in amounts of nuclear-encoded PSI subunits, such as PsaD
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed ArticleQuantification of banding patterns on Coomassie-stained 2D BN/SDS polyacrylamide (PA) gels revealed marked reductions in levels of the plastid-encoded PSII core subunits ... PsbC ... in ... prps1-1
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed Articlein prpl24-1 ... decrease in amounts of nuclear-encoded PSI subunits, such as PsaD
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed ArticleOther plastid-encoded subunits of thylakoid multiprotein complexes, including the reaction centre of PSI ... PsaA ... also showed marked declines in level in mutant plants, particularly in prpl24-1 homozygotes
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed Articlein ... prps17-1 ... Other plastid-encoded subunits of thylakoid multiprotein complexes, including ... the β-subunit of ATPase (ATPase β) ... also showed marked declines in level
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed ArticleQuantification of banding patterns on Coomassie-stained 2D BN/SDS polyacrylamide (PA) gels revealed marked reductions in levels of the plastid-encoded PSII core subunits PsbA ... in prpl24-1
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed Articleprps17-1 ... increase was also observed for rbcL mRNA
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed ArticleQuantification of banding patterns on Coomassie-stained 2D BN/SDS polyacrylamide (PA) gels revealed marked reductions in levels of the plastid-encoded PSII core subunits ... PsbB ... in ... prps17-1
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed ArticleQuantification of banding patterns on Coomassie-stained 2D BN/SDS polyacrylamide (PA) gels revealed marked reductions in levels of the plastid-encoded PSII core subunits ... PsbB ... in prpl24-1
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed ArticleOther plastid-encoded subunits of thylakoid multiprotein complexes, including ... cytochrome b6 (PetB ... also showed marked declines in level in mutant plants, particularly in prpl24-1 homozygotes
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed ArticleOther plastid-encoded subunits of thylakoid multiprotein complexes, including the reaction centre of PSI ... Psa ... B ... also showed marked declines in level in mutant plants, particularly in prpl24-1 homozygotes
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed Articlein ... prps17-1 ... Other plastid-encoded subunits of thylakoid multiprotein complexes, including ... the large subunit of Rubisco (RbcL) also showed marked declines in level
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed ArticleReal-time PCR analyses indicate that both PRPL1 splicing variants are present in leaves and developing siliques, with PRPL1.1 being more abundant than PRPL1.2
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed Articleprpl24-1 ... increase was also observed for rbcL mRNA
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed Articleprps17-1 ... The steady-state level of the psbA transcript was increased by a factor of almost two in mutant leaves relative to WT plants
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed Articleonly one transcript variant was detectable for PRPL4 (PRPL4.1) ... in both leaves and siliques
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed Articleprpl24-1 ... The steady-state level of the psbA transcript was increased by a factor of almost two in mutant leaves relative to WT plants
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed ArticleOther plastid-encoded subunits of thylakoid multiprotein complexes, including the ... β-subunit of ATPase (ATPase β) ... also showed marked declines in level in mutant plants, particularly in prpl24-1 homozygotes
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed Articlein prpl24-1 ... decrease in amounts of nuclear-encoded PSI subunits, such as ... PsaF
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed Articleprps1-1 ... The steady-state level of the psbA transcript was increased by a factor of almost two in mutant leaves relative to WT plants ... psaA–psaB and atpB–atpE operons, monitored by employing psaB- and atpB-specific probes
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed Articlein ... prps1-1 ... Other plastid-encoded subunits of thylakoid multiprotein complexes, including the ... cytochrome b6 (PetB ... also showed marked declines in level
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed ArticleQuantification of banding patterns on Coomassie-stained 2D BN/SDS polyacrylamide (PA) gels revealed marked reductions in levels of the plastid-encoded PSII core subunits PsbA ... in ... prps1-1
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed Articleprps1-1 ... increase was also observed for rbcL mRNA
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed Articlein ... prps17-1 ... decrease in amounts of nuclear-encoded PSI subunits, such as ... PsaF
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed Articlein ... prps1-1 ... decrease in amounts of nuclear-encoded PSI subunits, such as PsaD
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed Articleprpl24-1 ... increase was also observed for ... transcripts of the psaA–psaB and atpB–atpE operons, monitored by employing psaB- and atpB-specific probes
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed ArticleQuantification of banding patterns on Coomassie-stained 2D BN/SDS polyacrylamide (PA) gels revealed marked reductions in levels of the plastid-encoded PSII core subunits ... PsbD in ... prps1-1
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed Articlein prpl24-1 ... decrease in amounts of nuclear-encoded PSI subunits, such as ... PsaO
Romani I, Tadini L, Rossi F, Masiero S, Pribil M, Jahns P, Kater M, Leister D, Pesaresi P - Versatile roles of Arabidopsis plastid ribosomal proteins in plant growth and development
Visit the PubMed Articlein ftsHi1-1 mutants ... First, we examined the filament localization and morphology of FtsZ using immunofluorescence microscopy. We found that FtsZ localized to single mid-plastid rings as in the WT, and FtsZ filament morphology was not altered
Kadirjan-Kalbach DK, Yoder DW, Ruckle ME, Larkin RM, Osteryoung KW - FtsHi1/ARC1 is an essential gene in Arabidopsis that links chloroplast biogenesis and division
Visit the PubMed Articlebecause overexpression of the outer envelope protein PLASTID DIVISION 2 (PDV2) (Miyagishima et al., 2006) results in smaller and more numerous chloroplasts than in the WT (Okazaki et al., 2009), reminiscent of ftsHi1-1 (Pyke and Leech, 1992, 1994) (Figure 1a), we compared PDV2 protein levels in ftsHi1-1 and the WT by immunoblotting. No obvious difference in PDV2 level was detected at any stage tested
Kadirjan-Kalbach DK, Yoder DW, Ruckle ME, Larkin RM, Osteryoung KW - FtsHi1/ARC1 is an essential gene in Arabidopsis that links chloroplast biogenesis and division
Visit the PubMed ArticleTo test the localization of FtsHi1, we generated a 35S:FtsHi1-YFP construct and expressed it in ftsHi1-1. The transgene complemented the chloroplast morphology phenotype in several transgenic lines (Figure 2a and Figure S1), indicating the FtsHi1–yellow fluorescent protein (YFP) fusion protein is functional. In complemented lines, the YFP signal localized to the chloroplast periphery (Figure 2b, bottom), whereas in plants expressing a 35S:YFP control (Zhang et al., 2009) the YFP signal was in the cytosol and nucleus (Figure 2b, top). Combined with the presence of predicted transmembrane domains (Figure 1b), these results suggest that FtsHi1–YFP localizes to the chloroplast envelope, consistent with proteomic data at AT_CHLORO (Ferro et al., 2010) indicating that 99.6% of the FtsHi1 protein is in the envelope fraction. Based on the presence of the predicted transit peptide, it is likely that FtsHi1 resides in the inner envelope
Kadirjan-Kalbach DK, Yoder DW, Ruckle ME, Larkin RM, Osteryoung KW - FtsHi1/ARC1 is an essential gene in Arabidopsis that links chloroplast biogenesis and division
Visit the PubMed ArticleRpoTp/SCA3 was found significantly up-regulated in ... mda1-2 ... when compared with Col-0
Robles P, Micol JL, Quesada V - Arabidopsis MDA1, a nuclear-encoded protein, functions in chloroplast development and abiotic stress responses
Visit the PubMed ArticleRUG2 ... largely down-regulated in response ... to ... salt
Robles P, Micol JL, Quesada V - Arabidopsis MDA1, a nuclear-encoded protein, functions in chloroplast development and abiotic stress responses
Visit the PubMed ArticleSOLDAT10 ... largely down-regulated in response to ... salt
Robles P, Micol JL, Quesada V - Arabidopsis MDA1, a nuclear-encoded protein, functions in chloroplast development and abiotic stress responses
Visit the PubMed Articlein mda1-1 ... the expression of ... accD ... up-regulated
Robles P, Micol JL, Quesada V - Arabidopsis MDA1, a nuclear-encoded protein, functions in chloroplast development and abiotic stress responses
Visit the PubMed Articledown-regulation of MDA1 expression in mda1-1 ... compared with Col-0
Robles P, Micol JL, Quesada V - Arabidopsis MDA1, a nuclear-encoded protein, functions in chloroplast development and abiotic stress responses
Visit the PubMed ArticlepsbA was up-regulated in mda1-1 soldat10 compared with Col-0 ... or Ler
Robles P, Micol JL, Quesada V - Arabidopsis MDA1, a nuclear-encoded protein, functions in chloroplast development and abiotic stress responses
Visit the PubMed Articlein mda1-1 ... the expression of ... rps18 ... up-regulated
Robles P, Micol JL, Quesada V - Arabidopsis MDA1, a nuclear-encoded protein, functions in chloroplast development and abiotic stress responses
Visit the PubMed ArticleMDA1 transcripts ... highest in ... vegetative leaves
Robles P, Micol JL, Quesada V - Arabidopsis MDA1, a nuclear-encoded protein, functions in chloroplast development and abiotic stress responses
Visit the PubMed ArticleSOLDAT10 ... largely down-regulated in response to ABA
Robles P, Micol JL, Quesada V - Arabidopsis MDA1, a nuclear-encoded protein, functions in chloroplast development and abiotic stress responses
Visit the PubMed ArticleIn mda1-1 rug2-2 compared with Col-0 ... psbA ... up-regulated
Robles P, Micol JL, Quesada V - Arabidopsis MDA1, a nuclear-encoded protein, functions in chloroplast development and abiotic stress responses
Visit the PubMed ArticleRUG2 ... largely down-regulated in response to ABA
Robles P, Micol JL, Quesada V - Arabidopsis MDA1, a nuclear-encoded protein, functions in chloroplast development and abiotic stress responses
Visit the PubMed Articlein mda1-1 ... the expression of ... clpP [1.7-fold down-regulated
Robles P, Micol JL, Quesada V - Arabidopsis MDA1, a nuclear-encoded protein, functions in chloroplast development and abiotic stress responses
Visit the PubMed ArticleMDA1 transcripts were detected in all organs analyzed
Robles P, Micol JL, Quesada V - Arabidopsis MDA1, a nuclear-encoded protein, functions in chloroplast development and abiotic stress responses
Visit the PubMed ArticleRpoTp/SCA3 was found significantly up-regulated in mda1-1 ... when compared with Col-0
Robles P, Micol JL, Quesada V - Arabidopsis MDA1, a nuclear-encoded protein, functions in chloroplast development and abiotic stress responses
Visit the PubMed Articlein mda1-1 ... the expression of psbA ... up-regulated
Robles P, Micol JL, Quesada V - Arabidopsis MDA1, a nuclear-encoded protein, functions in chloroplast development and abiotic stress responses
Visit the PubMed Articlein mda1-1 ... the transcript levels of matk ... were only slightly affected
Robles P, Micol JL, Quesada V - Arabidopsis MDA1, a nuclear-encoded protein, functions in chloroplast development and abiotic stress responses
Visit the PubMed ArticleIn mda1-1 rug2-2 compared with Col-0, RpoTp/SCA3 ... up-regulated
Robles P, Micol JL, Quesada V - Arabidopsis MDA1, a nuclear-encoded protein, functions in chloroplast development and abiotic stress responses
Visit the PubMed Articledown-regulation of MDA1 expression in ... mda1-2 compared with Col-0
Robles P, Micol JL, Quesada V - Arabidopsis MDA1, a nuclear-encoded protein, functions in chloroplast development and abiotic stress responses
Visit the PubMed Articlein mda1-1 ... the transcript levels of ... rpoB ... were only slightly affected
Robles P, Micol JL, Quesada V - Arabidopsis MDA1, a nuclear-encoded protein, functions in chloroplast development and abiotic stress responses
Visit the PubMed ArticleIn ... lon1 ... mutants ... were increased in abundance according to DIGE and iTRAQ experiments ... monodehydroascorbate reductase (At1g63940 ... in lon1-2
Solheim C, Li L, Hatzopoulos P, Millar AH - Loss of Lon1 in Arabidopsis changes the mitochondrial proteome leading to altered metabolite profiles and growth retardation without an accumulation of oxidative damage
Visit the PubMed ArticleBetween both DIGE and iTRAQ data, lon1-1 had six different TCA cycle enzyme subunits that were increased in abundance including ... dihydrolipoamide S-acetyltransferase (At3g13930
Solheim C, Li L, Hatzopoulos P, Millar AH - Loss of Lon1 in Arabidopsis changes the mitochondrial proteome leading to altered metabolite profiles and growth retardation without an accumulation of oxidative damage
Visit the PubMed ArticleIn ... lon1 ... mutants, HSP60 (At2g33210 ... were increased in abundance according to DIGE and iTRAQ experiments
Solheim C, Li L, Hatzopoulos P, Millar AH - Loss of Lon1 in Arabidopsis changes the mitochondrial proteome leading to altered metabolite profiles and growth retardation without an accumulation of oxidative damage
Visit the PubMed ArticleBetween both DIGE and iTRAQ data, lon1-1 had six different TCA cycle enzyme subunits that were increased in abundance including ... succinyl-CoA ligase (At2f20420
Solheim C, Li L, Hatzopoulos P, Millar AH - Loss of Lon1 in Arabidopsis changes the mitochondrial proteome leading to altered metabolite profiles and growth retardation without an accumulation of oxidative damage
Visit the PubMed ArticleIn ... lon1-1 ... mutants ... were increased in abundance according to DIGE and iTRAQ experiments ... At3g27280
Solheim C, Li L, Hatzopoulos P, Millar AH - Loss of Lon1 in Arabidopsis changes the mitochondrial proteome leading to altered metabolite profiles and growth retardation without an accumulation of oxidative damage
Visit the PubMed ArticleBetween both DIGE and iTRAQ data, lon1-1 had six different TCA cycle enzyme subunits that were increased in abundance including ... isocitrate dehydrogenase (At3g09810
Solheim C, Li L, Hatzopoulos P, Millar AH - Loss of Lon1 in Arabidopsis changes the mitochondrial proteome leading to altered metabolite profiles and growth retardation without an accumulation of oxidative damage
Visit the PubMed ArticleIn ... lon1 ... mutants ... HSP70 ... At5g09590) were increased in abundance according to DIGE and iTRAQ experiments ... At5g09590
Solheim C, Li L, Hatzopoulos P, Millar AH - Loss of Lon1 in Arabidopsis changes the mitochondrial proteome leading to altered metabolite profiles and growth retardation without an accumulation of oxidative damage
Visit the PubMed ArticleBetween both DIGE and iTRAQ data, lon1-1 had ... had six different ... different TCA cycle enzyme subunits that were increased in abundance including; mitochondrial lipoamide dehydrogenase (At1g48030
Solheim C, Li L, Hatzopoulos P, Millar AH - Loss of Lon1 in Arabidopsis changes the mitochondrial proteome leading to altered metabolite profiles and growth retardation without an accumulation of oxidative damage
Visit the PubMed ArticleBetween both DIGE and iTRAQ data, lon1-1 had six different TCA cycle enzyme subunits that were increased in abundance including ... malate dehydrogenase (At1g53240
Solheim C, Li L, Hatzopoulos P, Millar AH - Loss of Lon1 in Arabidopsis changes the mitochondrial proteome leading to altered metabolite profiles and growth retardation without an accumulation of oxidative damage
Visit the PubMed ArticleThe lon1-2 mutant also had enzymes increased in abundance ... including ... 2-oxoglutarate dehydrogenase (At5g65750
Solheim C, Li L, Hatzopoulos P, Millar AH - Loss of Lon1 in Arabidopsis changes the mitochondrial proteome leading to altered metabolite profiles and growth retardation without an accumulation of oxidative damage
Visit the PubMed ArticleIn ... lon1 ... mutants ... several ... enzymes ... such as ... formate dehydrogenase (At5g14740 ... had lower abundances
Solheim C, Li L, Hatzopoulos P, Millar AH - Loss of Lon1 in Arabidopsis changes the mitochondrial proteome leading to altered metabolite profiles and growth retardation without an accumulation of oxidative damage
Visit the PubMed ArticleIn ... lon1-1 ... mutants ... were increased in abundance according to DIGE and iTRAQ ... experiments ... At5g40770
Solheim C, Li L, Hatzopoulos P, Millar AH - Loss of Lon1 in Arabidopsis changes the mitochondrial proteome leading to altered metabolite profiles and growth retardation without an accumulation of oxidative damage
Visit the PubMed ArticleIn ... lon1 ... mutants ... several enzymes ... such as glutamate dehydrogenase (At5g18170 ... had lower abundances
Solheim C, Li L, Hatzopoulos P, Millar AH - Loss of Lon1 in Arabidopsis changes the mitochondrial proteome leading to altered metabolite profiles and growth retardation without an accumulation of oxidative damage
Visit the PubMed ArticleIn ... lon1-1 ... 1 ... mutants ... were increased in abundance according to DIGE and iTRAQ ... At4g28510
Solheim C, Li L, Hatzopoulos P, Millar AH - Loss of Lon1 in Arabidopsis changes the mitochondrial proteome leading to altered metabolite profiles and growth retardation without an accumulation of oxidative damage
Visit the PubMed ArticleThe lon1-2 mutant also had enzymes increased in abundance ... including ... isocitrate dehydrogenase ... At5g03290
Solheim C, Li L, Hatzopoulos P, Millar AH - Loss of Lon1 in Arabidopsis changes the mitochondrial proteome leading to altered metabolite profiles and growth retardation without an accumulation of oxidative damage
Visit the PubMed ArticleIn ... lon1-1 ... mutants ... were increased in abundance according to DIGE and iTRAQ experiments ... At5g06290
Solheim C, Li L, Hatzopoulos P, Millar AH - Loss of Lon1 in Arabidopsis changes the mitochondrial proteome leading to altered metabolite profiles and growth retardation without an accumulation of oxidative damage
Visit the PubMed ArticleIn ... lon1 ... mutants ... HSP70 (At4g37910 ... were increased in abundance according to DIGE and iTRAQ experiments
Solheim C, Li L, Hatzopoulos P, Millar AH - Loss of Lon1 in Arabidopsis changes the mitochondrial proteome leading to altered metabolite profiles and growth retardation without an accumulation of oxidative damage
Visit the PubMed ArticleIn ... lon1 ... mutants ... HSP60 ... At3g23990 ... were increased in abundance according to DIGE and iTRAQ experiments
Solheim C, Li L, Hatzopoulos P, Millar AH - Loss of Lon1 in Arabidopsis changes the mitochondrial proteome leading to altered metabolite profiles and growth retardation without an accumulation of oxidative damage
Visit the PubMed ArticleIn ... lon1 ... mutants ... several enzymes ... such as ... GABA-T (4-aminobutyrate transaminase) (At3g22200 ... had lower abundances
Solheim C, Li L, Hatzopoulos P, Millar AH - Loss of Lon1 in Arabidopsis changes the mitochondrial proteome leading to altered metabolite profiles and growth retardation without an accumulation of oxidative damage
Visit the PubMed ArticleIn ... lon1 ... mutants ... several enzymes ... such as glutamate ... such ... dehydrogenase ... At5g07440 ... had lower abundances
Solheim C, Li L, Hatzopoulos P, Millar AH - Loss of Lon1 in Arabidopsis changes the mitochondrial proteome leading to altered metabolite profiles and growth retardation without an accumulation of oxidative damage
Visit the PubMed ArticleThe lon1-2 mutant also had enzymes increased in abundance including ... isocitrate dehydrogenase (At4g36260
Solheim C, Li L, Hatzopoulos P, Millar AH - Loss of Lon1 in Arabidopsis changes the mitochondrial proteome leading to altered metabolite profiles and growth retardation without an accumulation of oxidative damage
Visit the PubMed ArticleBetween both DIGE and iTRAQ data, lon1-1 had six different TCA cycle enzyme subunits that were increased in abundance including ... 2-oxoglutarate dehydrogenase (At3g55410
Solheim C, Li L, Hatzopoulos P, Millar AH - Loss of Lon1 in Arabidopsis changes the mitochondrial proteome leading to altered metabolite profiles and growth retardation without an accumulation of oxidative damage
Visit the PubMed ArticleThe lon1-2 mutant also had enzymes increased in abundance including ... 2-oxoglutarate dehydrogenase ... At3g55410
Solheim C, Li L, Hatzopoulos P, Millar AH - Loss of Lon1 in Arabidopsis changes the mitochondrial proteome leading to altered metabolite profiles and growth retardation without an accumulation of oxidative damage
Visit the PubMed ArticleThe transcript levels of ... AtARC5 ... were significantly reduced in ga1-3 relative to the wild type ... The application of exogenous GA restored the expression of chloroplast division genes in the ga1-3 mutant to approximately wild-type levels
Jiang X, Li H, Wang T, Peng C, Wang H, Wu H, Wang X - Gibberellin indirectly promotes chloroplast biogenesis as a means to maintain the chloroplast population of expanded cells
Visit the PubMed Articlethe transcriptional levels of all the selected chloroplast division genes in the della mutant were comparable with those of wild-type plants
Jiang X, Li H, Wang T, Peng C, Wang H, Wu H, Wang X - Gibberellin indirectly promotes chloroplast biogenesis as a means to maintain the chloroplast population of expanded cells
Visit the PubMed Articlethe transcript levels of ... AtFtsZ2-1 were reduced in gai-1
Jiang X, Li H, Wang T, Peng C, Wang H, Wu H, Wang X - Gibberellin indirectly promotes chloroplast biogenesis as a means to maintain the chloroplast population of expanded cells
Visit the PubMed Articlethe transcript levels of ... AtPDV2 ... were reduced in gai-1
Jiang X, Li H, Wang T, Peng C, Wang H, Wu H, Wang X - Gibberellin indirectly promotes chloroplast biogenesis as a means to maintain the chloroplast population of expanded cells
Visit the PubMed ArticleWe next performed quantitative RT-PCR to measure the transcript levels of the genes related to chloroplast division, cell division and cell expansion. Our results showed that the majority of transcriptional upregulation for selected chloroplast division genes and cell division genes occurred between hours 24 and 48 (Figure 4c,e), whereas the transcriptional upregulation for expansin genes AtEXPA1 ... occurred between hours 0 and 2 (Figure 4g), suggesting that the cell expansion-related genes tended to be upregulated much earlier and more significantly than genes related to chloroplast division and cell division
Jiang X, Li H, Wang T, Peng C, Wang H, Wu H, Wang X - Gibberellin indirectly promotes chloroplast biogenesis as a means to maintain the chloroplast population of expanded cells
Visit the PubMed ArticleThe transcript levels of ... AtARC6 ... were significantly reduced in ga1-3 relative to the wild type ... The application of exogenous GA restored the expression of chloroplast division genes in the ga1-3 mutant to approximately wild-type levels
Jiang X, Li H, Wang T, Peng C, Wang H, Wu H, Wang X - Gibberellin indirectly promotes chloroplast biogenesis as a means to maintain the chloroplast population of expanded cells
Visit the PubMed ArticleWe next performed quantitative RT-PCR to measure the transcript levels of the genes related to chloroplast division, cell division and cell expansion. Our results showed that the majority of transcriptional upregulation for selected chloroplast division genes and cell division genes occurred between hours 24 and 48 (Figure 4c,e), whereas the transcriptional upregulation for expansin genes ... AtEXPA8 occurred between hours 0 and 2 (Figure 4g), suggesting that the cell expansion-related genes tended to be upregulated much earlier and more significantly than genes related to chloroplast division and cell division
Jiang X, Li H, Wang T, Peng C, Wang H, Wu H, Wang X - Gibberellin indirectly promotes chloroplast biogenesis as a means to maintain the chloroplast population of expanded cells
Visit the PubMed Articlethe transcript levels of ... AtARC5 ... were reduced in gai-1
Jiang X, Li H, Wang T, Peng C, Wang H, Wu H, Wang X - Gibberellin indirectly promotes chloroplast biogenesis as a means to maintain the chloroplast population of expanded cells
Visit the PubMed ArticleThe transcript levels of AtPDV1 ... were significantly reduced in ga1-3 relative to the wild type ... The application of exogenous GA restored the expression of chloroplast division genes in the ga1-3 mutant to approximately wild-type levels
Jiang X, Li H, Wang T, Peng C, Wang H, Wu H, Wang X - Gibberellin indirectly promotes chloroplast biogenesis as a means to maintain the chloroplast population of expanded cells
Visit the PubMed Articlethe transcript levels of ... AtPDV1 ... were reduced in gai-1
Jiang X, Li H, Wang T, Peng C, Wang H, Wu H, Wang X - Gibberellin indirectly promotes chloroplast biogenesis as a means to maintain the chloroplast population of expanded cells
Visit the PubMed ArticleThe transcript levels of ... AtPDV2 ... were significantly reduced in ga1-3 relative to the wild type ... The application of exogenous GA restored the expression of chloroplast division genes in the ga1-3 mutant to approximately wild-type levels
Jiang X, Li H, Wang T, Peng C, Wang H, Wu H, Wang X - Gibberellin indirectly promotes chloroplast biogenesis as a means to maintain the chloroplast population of expanded cells
Visit the PubMed Articlethe transcript levels of all the selected chloroplast division genes in the della mutant are comparable with those of the wild type
Jiang X, Li H, Wang T, Peng C, Wang H, Wu H, Wang X - Gibberellin indirectly promotes chloroplast biogenesis as a means to maintain the chloroplast population of expanded cells
Visit the PubMed ArticleQuantitative RT-PCR analyses showed that the ARC5 level, but not those of other chloroplast division genes, was significantly increased in rga-24 gai-t6
Jiang X, Li H, Wang T, Peng C, Wang H, Wu H, Wang X - Gibberellin indirectly promotes chloroplast biogenesis as a means to maintain the chloroplast population of expanded cells
Visit the PubMed Articlequantitative RT-PCR analyses showed that the transcript levels of ARC5 in rga-24 gai-t6 can be partially inhibited by PAC
Jiang X, Li H, Wang T, Peng C, Wang H, Wu H, Wang X - Gibberellin indirectly promotes chloroplast biogenesis as a means to maintain the chloroplast population of expanded cells
Visit the PubMed ArticleThe transcript levels of ... AtFtsZ2-1 were significantly reduced in ga1-3 relative to the wild type ... The application of exogenous GA restored the expression of chloroplast division genes in the ga1-3 mutant to approximately wild-type levels
Jiang X, Li H, Wang T, Peng C, Wang H, Wu H, Wang X - Gibberellin indirectly promotes chloroplast biogenesis as a means to maintain the chloroplast population of expanded cells
Visit the PubMed ArticleTo understand the dynamics of BR homeostasis by auxin, we examined the kinetics of changes in expression of a set of BR-related genes. Upon auxin treatment, BRX, a positive regulator of BR biosynthesis (Mouchel et al., 2006), reached its highest induction 30–60 min after auxin treatment and then gradually diminished
Choi S, Cho YH, Kim K, Matsui M, Son SH, Kim SK, Fujioka S, Hwang I - BAT1, a putative acyltransferase, modulates brassinosteroid levels in Arabidopsis
Visit the PubMed ArticleWe further analyzed subcellular fractions containing the ER using anti-GFP antibodies for BAT1-GFP and anti-calreticulin (CRT) antibodies for the ER marker (Figure S1d). Consistent with BAT1-GFP imaging, BAT1 proteins were detected in the ER fraction
Choi S, Cho YH, Kim K, Matsui M, Son SH, Kim SK, Fujioka S, Hwang I - BAT1, a putative acyltransferase, modulates brassinosteroid levels in Arabidopsis
Visit the PubMed ArticleWe then determined the expression pattern of BAT1 using transgenic lines harboring a transcriptional reporter construct (proBAT1:GUS) in which 2.0 kb of the BAT1 promoter was fused to the ß-glucuronidase (GUS) gene. Promoter activity was found to be present in most organs, especially young tissues and vascular bundles (Figure 3b–d). In young seedlings, GUS staining was mainly observed in the vascular cylinder of ... leaves
Choi S, Cho YH, Kim K, Matsui M, Son SH, Kim SK, Fujioka S, Hwang I - BAT1, a putative acyltransferase, modulates brassinosteroid levels in Arabidopsis
Visit the PubMed Articleauxin-dependent activation of BAT1 was nearly abolished in tir1-1 (Figure 6d), a loss-of-function mutant of the TIR1 auxin receptor
Choi S, Cho YH, Kim K, Matsui M, Son SH, Kim SK, Fujioka S, Hwang I - BAT1, a putative acyltransferase, modulates brassinosteroid levels in Arabidopsis
Visit the PubMed ArticleTo understand the dynamics of BR homeostasis by auxin, we examined the kinetics of changes in expression of a set of BR-related genes ... induction of the UGT73C5 ... that encode the UDP-glycosyltransferases involved in BR inactivation by glucosylation began to slightly increase 3 h after auxin application and then decreased
Choi S, Cho YH, Kim K, Matsui M, Son SH, Kim SK, Fujioka S, Hwang I - BAT1, a putative acyltransferase, modulates brassinosteroid levels in Arabidopsis
Visit the PubMed ArticleTo understand the dynamics of BR homeostasis by auxin, we examined the kinetics of changes in expression of a set of BR-related genes ... induction of the ... UGT73C6 ... that encode the UDP-glycosyltransferases involved in BR inactivation by glucosylation began to slightly increase 3 h after auxin application and then decreased
Choi S, Cho YH, Kim K, Matsui M, Son SH, Kim SK, Fujioka S, Hwang I - BAT1, a putative acyltransferase, modulates brassinosteroid levels in Arabidopsis
Visit the PubMed ArticleTo understand the dynamics of BR homeostasis by auxin, we examined the kinetics of changes in expression of a set of BR-related genes ... the induction of BAT1 was slightly delayed, reaching its peak at 60–90 min after auxin treatment
Choi S, Cho YH, Kim K, Matsui M, Son SH, Kim SK, Fujioka S, Hwang I - BAT1, a putative acyltransferase, modulates brassinosteroid levels in Arabidopsis
Visit the PubMed Articlewe first examined its subcellular localization using GFP-tagged BAT1 proteins and confocal laser scanning microscopy. BAT1-GFP co-localized with RFP-tagged BiP, an ER marker protein
Choi S, Cho YH, Kim K, Matsui M, Son SH, Kim SK, Fujioka S, Hwang I - BAT1, a putative acyltransferase, modulates brassinosteroid levels in Arabidopsis
Visit the PubMed ArticleWe then determined the expression pattern of BAT1 using transgenic lines harboring a transcriptional reporter construct (proBAT1:GUS) in which 2.0 kb of the BAT1 promoter was fused to the ß-glucuronidase (GUS) gene. Promoter activity was found to be present in most organs, especially young tissues and vascular bundles (Figure 3b–d). In young seedlings, GUS staining was mainly observed in the vascular cylinder of cotyledons
Choi S, Cho YH, Kim K, Matsui M, Son SH, Kim SK, Fujioka S, Hwang I - BAT1, a putative acyltransferase, modulates brassinosteroid levels in Arabidopsis
Visit the PubMed ArticleTo understand the dynamics of BR homeostasis by auxin, we examined the kinetics of changes in expression of a set of BR-related genes ... The transcript of SHK1, the closest homolog of BAS1, was highly induced at 3 h after auxin application, and this induction was sustained until 24 h after auxin treatment
Choi S, Cho YH, Kim K, Matsui M, Son SH, Kim SK, Fujioka S, Hwang I - BAT1, a putative acyltransferase, modulates brassinosteroid levels in Arabidopsis
Visit the PubMed ArticleBAT1 expression was significantly induced by auxin
Choi S, Cho YH, Kim K, Matsui M, Son SH, Kim SK, Fujioka S, Hwang I - BAT1, a putative acyltransferase, modulates brassinosteroid levels in Arabidopsis
Visit the PubMed Articleexpression of BAT1 was substantially decreased in the loss-of-function arf19-1 mutant
Choi S, Cho YH, Kim K, Matsui M, Son SH, Kim SK, Fujioka S, Hwang I - BAT1, a putative acyltransferase, modulates brassinosteroid levels in Arabidopsis
Visit the PubMed ArticleTo understand the dynamics of BR homeostasis by auxin, we examined the kinetics of changes in expression of a set of BR-related genes. Upon auxin treatment, BRX, a positive regulator of BR biosynthesis (Mouchel et al., 2006), reached its highest induction 30–60 min after auxin treatment and then gradually diminished (Figure 6e). BAS1, encoding a BR inactivation enzyme involved in hydroxylation of active BRs, showed a similar activation pattern to BRX upon auxin application. However, at 3 h after auxin treatment, levels of the BAS1 transcript started to increase again
Choi S, Cho YH, Kim K, Matsui M, Son SH, Kim SK, Fujioka S, Hwang I - BAT1, a putative acyltransferase, modulates brassinosteroid levels in Arabidopsis
Visit the PubMed ArticleBAT1-GFP ... localized in the nucleus
Choi S, Cho YH, Kim K, Matsui M, Son SH, Kim SK, Fujioka S, Hwang I - BAT1, a putative acyltransferase, modulates brassinosteroid levels in Arabidopsis
Visit the PubMed ArticleThe expression of BAT1 was slightly, but not significantly, up-regulated by epiBL
Choi S, Cho YH, Kim K, Matsui M, Son SH, Kim SK, Fujioka S, Hwang I - BAT1, a putative acyltransferase, modulates brassinosteroid levels in Arabidopsis
Visit the PubMed ArticleOptical sections through pSUC5/tmGFP9 embryos confined pSUC5 activity specifically on the epidermis of the outer surface of the cotyledons
Pommerrenig B, Popko J, Heilmann M, Schulmeister S, Dietel K, Schmitt B, Stadler R, Feussner I, Sauer N - SUCROSE TRANSPORTER 5 supplies Arabidopsis embryos with biotin and affects triacylglycerol accumulation
Visit the PubMed ArticleWe generated two pSUC5/reporter lines under the control of a 2030 bp SUC5 promoter ... we observed pSUC5 activity for both constructs during the later stages of embryo development
Pommerrenig B, Popko J, Heilmann M, Schulmeister S, Dietel K, Schmitt B, Stadler R, Feussner I, Sauer N - SUCROSE TRANSPORTER 5 supplies Arabidopsis embryos with biotin and affects triacylglycerol accumulation
Visit the PubMed ArticleIn addition, we analysed the subcellular localization of the SUC5 protein by transiently transforming Arabidopsis protoplasts with N- and C-terminal GFP fusion constructs of SUC5. Both fusion proteins localized to the plasma membrane (Figure 1j,k). We verified this localization in particle-bombarded leek (Allium ampeloprasum) epidermis cells (Figure 1l,m)
Pommerrenig B, Popko J, Heilmann M, Schulmeister S, Dietel K, Schmitt B, Stadler R, Feussner I, Sauer N - SUCROSE TRANSPORTER 5 supplies Arabidopsis embryos with biotin and affects triacylglycerol accumulation
Visit the PubMed ArticleIn agreement with the pWUS:GUS reporter expression, in situ RNA hybridization using a WUS probe showed multiple ectopic foci of WUS expression on independent sections of a single 10-day-old minu1 minu2 meristem, including one in the axil of the cotyledon (Figure 4f). WUS expression was generally absent from its normal domain of expression. We propose that the individual foci observed by in situ hybridization together form the broad domain of expression observed by whole-mount analysis with pWUS:GUS
Sang Y, Silva-Ortega CO, Wu S, Yamaguchi N, Wu MF, Pfluger J, Gillmor CS, Gallagher KL, Wagner D - Mutations in two non-canonical Arabidopsis SWI2/SNF2 chromatin remodeling ATPases cause embryogenesis and stem cell maintenance defects
Visit the PubMed Articlewe conducted quantitative RT-PCR (qRT-PCR) using RNA extracted from dissected shoot apices of 6-day-old wild-type and minu1 minu2 seedlings ... CLV3 ... transcript levels were reduced to one-third ... of wild-type levels
Sang Y, Silva-Ortega CO, Wu S, Yamaguchi N, Wu MF, Pfluger J, Gillmor CS, Gallagher KL, Wagner D - Mutations in two non-canonical Arabidopsis SWI2/SNF2 chromatin remodeling ATPases cause embryogenesis and stem cell maintenance defects
Visit the PubMed ArticleConsistent with the public RNA expression data, GFP-tagged MINU2 protein was expressed in embryos, in the RAM and in the SAM
Sang Y, Silva-Ortega CO, Wu S, Yamaguchi N, Wu MF, Pfluger J, Gillmor CS, Gallagher KL, Wagner D - Mutations in two non-canonical Arabidopsis SWI2/SNF2 chromatin remodeling ATPases cause embryogenesis and stem cell maintenance defects
Visit the PubMed ArticleWe observed reduced expression for ... pARR7:GUS in the SAM and in the RAM of minu1 minu2, relative to the wild type, at day 6
Sang Y, Silva-Ortega CO, Wu S, Yamaguchi N, Wu MF, Pfluger J, Gillmor CS, Gallagher KL, Wagner D - Mutations in two non-canonical Arabidopsis SWI2/SNF2 chromatin remodeling ATPases cause embryogenesis and stem cell maintenance defects
Visit the PubMed Articlewe conducted quantitative RT-PCR (qRT-PCR) using RNA extracted from dissected shoot apices of 6-day-old wild-type and minu1 minu2 seedlings (Figure 4c). WUS expression was greatly elevated (about eightfold higher than wild-type levels)
Sang Y, Silva-Ortega CO, Wu S, Yamaguchi N, Wu MF, Pfluger J, Gillmor CS, Gallagher KL, Wagner D - Mutations in two non-canonical Arabidopsis SWI2/SNF2 chromatin remodeling ATPases cause embryogenesis and stem cell maintenance defects
Visit the PubMed Articlewe conducted quantitative RT-PCR (qRT-PCR) using RNA extracted from dissected shoot apices of 6-day-old wild-type and minu1 minu2 seedlings ... STM mRNA expression was increased to about twofold of that in the wild type
Sang Y, Silva-Ortega CO, Wu S, Yamaguchi N, Wu MF, Pfluger J, Gillmor CS, Gallagher KL, Wagner D - Mutations in two non-canonical Arabidopsis SWI2/SNF2 chromatin remodeling ATPases cause embryogenesis and stem cell maintenance defects
Visit the PubMed ArticleMINU2 ... expressed in all rapidly dividing cells, including those in the embryo, and in the RAM and SAM
Sang Y, Silva-Ortega CO, Wu S, Yamaguchi N, Wu MF, Pfluger J, Gillmor CS, Gallagher KL, Wagner D - Mutations in two non-canonical Arabidopsis SWI2/SNF2 chromatin remodeling ATPases cause embryogenesis and stem cell maintenance defects
Visit the PubMed ArticleMINU1 ... expressed in all rapidly dividing cells, including those in the embryo, and in the RAM and SAM
Sang Y, Silva-Ortega CO, Wu S, Yamaguchi N, Wu MF, Pfluger J, Gillmor CS, Gallagher KL, Wagner D - Mutations in two non-canonical Arabidopsis SWI2/SNF2 chromatin remodeling ATPases cause embryogenesis and stem cell maintenance defects
Visit the PubMed Articlewe conducted quantitative RT-PCR (qRT-PCR) using RNA extracted from dissected shoot apices of 6-day-old wild-type and minu1 minu2 seedlings ... expression of a marker of organ initiation, AINTEGUMENTA (ANT; Long and Barton, 2000), was much reduced in minu1 minu2 (to one-fifth of wild-type levels), consistent with the strong delay in initiation of the first true leaves in the double mutant (Figures 3a and 4a
Sang Y, Silva-Ortega CO, Wu S, Yamaguchi N, Wu MF, Pfluger J, Gillmor CS, Gallagher KL, Wagner D - Mutations in two non-canonical Arabidopsis SWI2/SNF2 chromatin remodeling ATPases cause embryogenesis and stem cell maintenance defects
Visit the PubMed Articlewe examined pWUS:GUS ... accumulation in 4- and 5-day-old minu1 minu2 shoot apices. We found no detectable pWUS:GUS ... signal at day 4, with weak pWUS:GUS ... expression visible at day 5
Sang Y, Silva-Ortega CO, Wu S, Yamaguchi N, Wu MF, Pfluger J, Gillmor CS, Gallagher KL, Wagner D - Mutations in two non-canonical Arabidopsis SWI2/SNF2 chromatin remodeling ATPases cause embryogenesis and stem cell maintenance defects
Visit the PubMed ArticleWe observed reduced expression for pARR5:GUS ... in the SAM and in the RAM of minu1 minu2, relative to the wild type, at day 6
Sang Y, Silva-Ortega CO, Wu S, Yamaguchi N, Wu MF, Pfluger J, Gillmor CS, Gallagher KL, Wagner D - Mutations in two non-canonical Arabidopsis SWI2/SNF2 chromatin remodeling ATPases cause embryogenesis and stem cell maintenance defects
Visit the PubMed Articlewe examined ... pCLV3:GUS accumulation in 4- and 5-day-old minu1 minu2 shoot apices. We found no detectable ... pCLV3:GUS signal at day 4 ... no pCLV3:GUS expression visible at day 5
Sang Y, Silva-Ortega CO, Wu S, Yamaguchi N, Wu MF, Pfluger J, Gillmor CS, Gallagher KL, Wagner D - Mutations in two non-canonical Arabidopsis SWI2/SNF2 chromatin remodeling ATPases cause embryogenesis and stem cell maintenance defects
Visit the PubMed ArticleExpression of pCLV3:GUS, by contrast, was barely detectable in 6-day-old minu1 minu2 SAMs
Sang Y, Silva-Ortega CO, Wu S, Yamaguchi N, Wu MF, Pfluger J, Gillmor CS, Gallagher KL, Wagner D - Mutations in two non-canonical Arabidopsis SWI2/SNF2 chromatin remodeling ATPases cause embryogenesis and stem cell maintenance defects
Visit the PubMed ArticleGFP-MINU2 was nuclear localized
Sang Y, Silva-Ortega CO, Wu S, Yamaguchi N, Wu MF, Pfluger J, Gillmor CS, Gallagher KL, Wagner D - Mutations in two non-canonical Arabidopsis SWI2/SNF2 chromatin remodeling ATPases cause embryogenesis and stem cell maintenance defects
Visit the PubMed ArticleIn 6-day-old minu1 minu2 SAMs, pWUS:GUS was expressed in an expanded domain compared with the wild type (Figure 4d). The extent of the ectopic WUS expression varied between individual minu1 minu2 plants
Sang Y, Silva-Ortega CO, Wu S, Yamaguchi N, Wu MF, Pfluger J, Gillmor CS, Gallagher KL, Wagner D - Mutations in two non-canonical Arabidopsis SWI2/SNF2 chromatin remodeling ATPases cause embryogenesis and stem cell maintenance defects
Visit the PubMed Articlewe conducted quantitative RT-PCR (qRT-PCR) using RNA extracted from dissected shoot apices of 6-day-old wild-type and minu1 minu2 seedlings ... CLV1 transcript levels were reduced to ... half of wild-type levels
Sang Y, Silva-Ortega CO, Wu S, Yamaguchi N, Wu MF, Pfluger J, Gillmor CS, Gallagher KL, Wagner D - Mutations in two non-canonical Arabidopsis SWI2/SNF2 chromatin remodeling ATPases cause embryogenesis and stem cell maintenance defects
Visit the PubMed ArticleThe expression levels ... of ... CBF1/DREB1b ... increased within 3 h in WT plants ... at 4°C
Nakai Y, Nakahira Y, Sumida H, Takebayashi K, Nagasawa Y, Yamasaki K, Akiyama M, Ohme-Takagi M, Fujiwara S, Shiina T, Mitsuda N, Fukusaki E, Kubo Y, Sato MH - Vascular plant one-zinc-finger protein 1/2 transcription factors regulate abiotic and biotic stress responses in Arabidopsis
Visit the PubMed ArticleThe expression levels ... of ... CBF2/DREB1c ... increased within 3 h in WT plants ... at 4°C
Nakai Y, Nakahira Y, Sumida H, Takebayashi K, Nagasawa Y, Yamasaki K, Akiyama M, Ohme-Takagi M, Fujiwara S, Shiina T, Mitsuda N, Fukusaki E, Kubo Y, Sato MH - Vascular plant one-zinc-finger protein 1/2 transcription factors regulate abiotic and biotic stress responses in Arabidopsis
Visit the PubMed Articlewe choose several genes that were significantly up-regulated or down-regulated in voz1voz2-2 in the microarray experiment, and compared the results of the expression level changes in these genes in WT, voz1voz2-1, voz1voz2-2 plants, and in a genomic complementation line that expressed VOZ1 (gVOZ1 #3) using quantitative RT-PCR (qRT-PCR ... the mitogen-activated protein (MAP) kinase family protein At4 g12020 ... significantly down-regulated
Nakai Y, Nakahira Y, Sumida H, Takebayashi K, Nagasawa Y, Yamasaki K, Akiyama M, Ohme-Takagi M, Fujiwara S, Shiina T, Mitsuda N, Fukusaki E, Kubo Y, Sato MH - Vascular plant one-zinc-finger protein 1/2 transcription factors regulate abiotic and biotic stress responses in Arabidopsis
Visit the PubMed ArticleDuring the cold treatment, the expression of the cold-responsive ... RD29A ... also increased considerably, even though CBF/DREB1s (CBF1-CBF3) gene expression was reduced in voz1voz2-2
Nakai Y, Nakahira Y, Sumida H, Takebayashi K, Nagasawa Y, Yamasaki K, Akiyama M, Ohme-Takagi M, Fujiwara S, Shiina T, Mitsuda N, Fukusaki E, Kubo Y, Sato MH - Vascular plant one-zinc-finger protein 1/2 transcription factors regulate abiotic and biotic stress responses in Arabidopsis
Visit the PubMed ArticleCBF2 expression levels in voz1voz2-2 were severely suppressed ... at 4°C
Nakai Y, Nakahira Y, Sumida H, Takebayashi K, Nagasawa Y, Yamasaki K, Akiyama M, Ohme-Takagi M, Fujiwara S, Shiina T, Mitsuda N, Fukusaki E, Kubo Y, Sato MH - Vascular plant one-zinc-finger protein 1/2 transcription factors regulate abiotic and biotic stress responses in Arabidopsis
Visit the PubMed Articlewe choose several genes that were significantly up-regulated or down-regulated in voz1voz2-2 in the microarray experiment, and compared the results of the expression level changes in these genes in WT, voz1voz2-1, voz1voz2-2 plants, and in a genomic complementation line that expressed VOZ1 (gVOZ1 #3) using quantitative RT-PCR (qRT-PCR ... up-regulated in both voz1voz2-1 and voz1voz2-2 ... APX2
Nakai Y, Nakahira Y, Sumida H, Takebayashi K, Nagasawa Y, Yamasaki K, Akiyama M, Ohme-Takagi M, Fujiwara S, Shiina T, Mitsuda N, Fukusaki E, Kubo Y, Sato MH - Vascular plant one-zinc-finger protein 1/2 transcription factors regulate abiotic and biotic stress responses in Arabidopsis
Visit the PubMed ArticleChanges in ... VOZ2 expression levels at a chilling temperature were examined using qRT-PCR. WT Arabidopsis seedlings were grown at 22°C and were then transferred to an environment that was maintained at 4°C, and VOZs expression was measured at different time points. The expression of these genes decreased after 3 h at 4°C, and the transcription of both genes almost disappeared after 24 h of cold treatment (Figure 2a
Nakai Y, Nakahira Y, Sumida H, Takebayashi K, Nagasawa Y, Yamasaki K, Akiyama M, Ohme-Takagi M, Fujiwara S, Shiina T, Mitsuda N, Fukusaki E, Kubo Y, Sato MH - Vascular plant one-zinc-finger protein 1/2 transcription factors regulate abiotic and biotic stress responses in Arabidopsis
Visit the PubMed Articlewe choose several genes that were significantly up-regulated or down-regulated in voz1voz2-2 in the microarray experiment, and compared the results of the expression level changes in these genes in WT, voz1voz2-1, voz1voz2-2 plants, and in a genomic complementation line that expressed VOZ1 (gVOZ1 #3) using quantitative RT-PCR (qRT-PCR ... disease-related ... At5 g40919 ... significantly down-regulated
Nakai Y, Nakahira Y, Sumida H, Takebayashi K, Nagasawa Y, Yamasaki K, Akiyama M, Ohme-Takagi M, Fujiwara S, Shiina T, Mitsuda N, Fukusaki E, Kubo Y, Sato MH - Vascular plant one-zinc-finger protein 1/2 transcription factors regulate abiotic and biotic stress responses in Arabidopsis
Visit the PubMed Articlewe choose several genes that were significantly up-regulated or down-regulated in voz1voz2-2 in the microarray experiment, and compared the results of the expression level changes in these genes in WT, voz1voz2-1, voz1voz2-2 plants, and in a genomic complementation line that expressed VOZ1 (gVOZ1 #3) using quantitative RT-PCR (qRT-PCR ... disease-related ... At4 g16950 ... significantly down-regulated
Nakai Y, Nakahira Y, Sumida H, Takebayashi K, Nagasawa Y, Yamasaki K, Akiyama M, Ohme-Takagi M, Fujiwara S, Shiina T, Mitsuda N, Fukusaki E, Kubo Y, Sato MH - Vascular plant one-zinc-finger protein 1/2 transcription factors regulate abiotic and biotic stress responses in Arabidopsis
Visit the PubMed Articleexpression levels in voz1voz2-2 ... of CBF3 was slightly suppressed at 4°C
Nakai Y, Nakahira Y, Sumida H, Takebayashi K, Nagasawa Y, Yamasaki K, Akiyama M, Ohme-Takagi M, Fujiwara S, Shiina T, Mitsuda N, Fukusaki E, Kubo Y, Sato MH - Vascular plant one-zinc-finger protein 1/2 transcription factors regulate abiotic and biotic stress responses in Arabidopsis
Visit the PubMed ArticleDuring the cold treatment, the expression of the cold-responsive ... RD29B also increased considerably, even though CBF/DREB1s (CBF1-CBF3) gene expression was reduced in voz1voz2-2
Nakai Y, Nakahira Y, Sumida H, Takebayashi K, Nagasawa Y, Yamasaki K, Akiyama M, Ohme-Takagi M, Fujiwara S, Shiina T, Mitsuda N, Fukusaki E, Kubo Y, Sato MH - Vascular plant one-zinc-finger protein 1/2 transcription factors regulate abiotic and biotic stress responses in Arabidopsis
Visit the PubMed ArticleDuring the cold treatment, the expression of the cold-responsive ... COR15A ... also increased considerably, even though CBF/DREB1s (CBF1-CBF3) gene expression was reduced in voz1voz2-2
Nakai Y, Nakahira Y, Sumida H, Takebayashi K, Nagasawa Y, Yamasaki K, Akiyama M, Ohme-Takagi M, Fujiwara S, Shiina T, Mitsuda N, Fukusaki E, Kubo Y, Sato MH - Vascular plant one-zinc-finger protein 1/2 transcription factors regulate abiotic and biotic stress responses in Arabidopsis
Visit the PubMed Articlewe choose several genes that were significantly up-regulated or down-regulated in voz1voz2-2 in the microarray experiment, and compared the results of the expression level changes in these genes in WT, voz1voz2-1, voz1voz2-2 plants, and in a genomic complementation line that expressed VOZ1 (gVOZ1 #3) using quantitative RT-PCR (qRT-PCR ... receptor-like kinase ... At1 g63590 ... significantly down-regulated
Nakai Y, Nakahira Y, Sumida H, Takebayashi K, Nagasawa Y, Yamasaki K, Akiyama M, Ohme-Takagi M, Fujiwara S, Shiina T, Mitsuda N, Fukusaki E, Kubo Y, Sato MH - Vascular plant one-zinc-finger protein 1/2 transcription factors regulate abiotic and biotic stress responses in Arabidopsis
Visit the PubMed Articlewe choose several genes that were significantly up-regulated or down-regulated in voz1voz2-2 in the microarray experiment, and compared the results of the expression level changes in these genes in WT, voz1voz2-1, voz1voz2-2 plants, and in a genomic complementation line that expressed VOZ1 (gVOZ1 #3) using quantitative RT-PCR (qRT-PCR ... up-regulated in both voz1voz2-1 and voz1voz2-2 ... HSP21
Nakai Y, Nakahira Y, Sumida H, Takebayashi K, Nagasawa Y, Yamasaki K, Akiyama M, Ohme-Takagi M, Fujiwara S, Shiina T, Mitsuda N, Fukusaki E, Kubo Y, Sato MH - Vascular plant one-zinc-finger protein 1/2 transcription factors regulate abiotic and biotic stress responses in Arabidopsis
Visit the PubMed ArticleThe expression levels ... of ... CBF3/DREB1a ... increased within 3 h in WT plants ... at 4°C
Nakai Y, Nakahira Y, Sumida H, Takebayashi K, Nagasawa Y, Yamasaki K, Akiyama M, Ohme-Takagi M, Fujiwara S, Shiina T, Mitsuda N, Fukusaki E, Kubo Y, Sato MH - Vascular plant one-zinc-finger protein 1/2 transcription factors regulate abiotic and biotic stress responses in Arabidopsis
Visit the PubMed ArticleConsistent with the VOZ2 transcription level, the VOZ2 protein was gradually degraded at 4°C and had almost disappeared after 24 h at 4°C, however treatment with a 26S proteasome inhibitor, MG132, before the cold treatment strongly inhibited VOZ2 degradation (Figure 2b). The ubiquitinated green fluorescent protein (GFP)-fused VOZ2 was observed not only under cold conditions and but also under normal growth conditions, although GFP–VOZ2 was further ubiquitinated under cold-stress conditions in the presence of MG132 (Figure S5), a finding that suggested that VOZ2 is constantly ubiquitinated and degraded not only under cold conditions but also under normal growth conditions
Nakai Y, Nakahira Y, Sumida H, Takebayashi K, Nagasawa Y, Yamasaki K, Akiyama M, Ohme-Takagi M, Fujiwara S, Shiina T, Mitsuda N, Fukusaki E, Kubo Y, Sato MH - Vascular plant one-zinc-finger protein 1/2 transcription factors regulate abiotic and biotic stress responses in Arabidopsis
Visit the PubMed Articlethe expression patterns of ... an abscisic acid-mediated abiotic (ABA)-mediated transcription factor CBF4/DREB1d ... up-regulated in voz1voz2-2 ... consistent ‘strong’ CBF4/DREB1d expression was observed not only at 4°C but also under normal growth conditions in voz1voz2-2
Nakai Y, Nakahira Y, Sumida H, Takebayashi K, Nagasawa Y, Yamasaki K, Akiyama M, Ohme-Takagi M, Fujiwara S, Shiina T, Mitsuda N, Fukusaki E, Kubo Y, Sato MH - Vascular plant one-zinc-finger protein 1/2 transcription factors regulate abiotic and biotic stress responses in Arabidopsis
Visit the PubMed Articlewe choose several genes that were significantly up-regulated or down-regulated in voz1voz2-2 in the microarray experiment, and compared the results of the expression level changes in these genes in WT, voz1voz2-1, voz1voz2-2 plants, and in a genomic complementation line that expressed VOZ1 (gVOZ1 #3) using quantitative RT-PCR (qRT-PCR ... receptor-like kinase ... At2 g25440 ... significantly down-regulated
Nakai Y, Nakahira Y, Sumida H, Takebayashi K, Nagasawa Y, Yamasaki K, Akiyama M, Ohme-Takagi M, Fujiwara S, Shiina T, Mitsuda N, Fukusaki E, Kubo Y, Sato MH - Vascular plant one-zinc-finger protein 1/2 transcription factors regulate abiotic and biotic stress responses in Arabidopsis
Visit the PubMed Articlewe choose several genes that were significantly up-regulated or down-regulated in voz1voz2-2 in the microarray experiment, and compared the results of the expression level changes in these genes in WT, voz1voz2-1, voz1voz2-2 plants, and in a genomic complementation line that expressed VOZ1 (gVOZ1 #3) using quantitative RT-PCR (qRT-PCR ... up-regulated in both voz1voz2-1 and voz1voz2-2 ... RAS1
Nakai Y, Nakahira Y, Sumida H, Takebayashi K, Nagasawa Y, Yamasaki K, Akiyama M, Ohme-Takagi M, Fujiwara S, Shiina T, Mitsuda N, Fukusaki E, Kubo Y, Sato MH - Vascular plant one-zinc-finger protein 1/2 transcription factors regulate abiotic and biotic stress responses in Arabidopsis
Visit the PubMed ArticleChanges in VOZ1 ... expression levels at a chilling temperature were examined using qRT-PCR. WT Arabidopsis seedlings were grown at 22°C and were then transferred to an environment that was maintained at 4°C, and VOZs expression was measured at different time points. The expression of these genes decreased after 3 h at 4°C, and the transcription of both genes almost disappeared after 24 h of cold treatment
Nakai Y, Nakahira Y, Sumida H, Takebayashi K, Nagasawa Y, Yamasaki K, Akiyama M, Ohme-Takagi M, Fujiwara S, Shiina T, Mitsuda N, Fukusaki E, Kubo Y, Sato MH - Vascular plant one-zinc-finger protein 1/2 transcription factors regulate abiotic and biotic stress responses in Arabidopsis
Visit the PubMed Articlewe choose several genes that were significantly up-regulated or down-regulated in voz1voz2-2 in the microarray experiment, and compared the results of the expression level changes in these genes in WT, voz1voz2-1, voz1voz2-2 plants, and in a genomic complementation line that expressed VOZ1 (gVOZ1 #3) using quantitative RT-PCR (qRT-PCR ... disease-related ... At5 g22690 ... significantly down-regulated
Nakai Y, Nakahira Y, Sumida H, Takebayashi K, Nagasawa Y, Yamasaki K, Akiyama M, Ohme-Takagi M, Fujiwara S, Shiina T, Mitsuda N, Fukusaki E, Kubo Y, Sato MH - Vascular plant one-zinc-finger protein 1/2 transcription factors regulate abiotic and biotic stress responses in Arabidopsis
Visit the PubMed ArticleCBF1 ... expression levels in voz1voz2-2 were severely suppressed ... at 4°C
Nakai Y, Nakahira Y, Sumida H, Takebayashi K, Nagasawa Y, Yamasaki K, Akiyama M, Ohme-Takagi M, Fujiwara S, Shiina T, Mitsuda N, Fukusaki E, Kubo Y, Sato MH - Vascular plant one-zinc-finger protein 1/2 transcription factors regulate abiotic and biotic stress responses in Arabidopsis
Visit the PubMed Articlethe expression patterns of a CBF/DREB1-independent cold-regulatory gene, ZAT12 ... up-regulated in voz1voz2-2. ‘Strong’ ZAT12 expression was rapidly induced within 30 min at 4°C and retained for over 24 h
Nakai Y, Nakahira Y, Sumida H, Takebayashi K, Nagasawa Y, Yamasaki K, Akiyama M, Ohme-Takagi M, Fujiwara S, Shiina T, Mitsuda N, Fukusaki E, Kubo Y, Sato MH - Vascular plant one-zinc-finger protein 1/2 transcription factors regulate abiotic and biotic stress responses in Arabidopsis
Visit the PubMed ArticleWe performed protein gel-blot analyses with specific antibodies ... Levels of ... mLPD ... were not influenced by loss of the BOU protein
Eisenhut M, Planchais S, Cabassa C, Guivarc'h A, Justin AM, Taconnat L, Renou JP, Linka M, Gagneul D, Timm S, Bauwe H, Carol P, Weber AP - Arabidopsis A BOUT DE SOUFFLE is a putative mitochondrial transporter involved in photorespiratory metabolism and is required for meristem growth at ambient CO2 levels
Visit the PubMed ArticleHIS2A ... strongly repressed in bou-2
Eisenhut M, Planchais S, Cabassa C, Guivarc'h A, Justin AM, Taconnat L, Renou JP, Linka M, Gagneul D, Timm S, Bauwe H, Carol P, Weber AP - Arabidopsis A BOUT DE SOUFFLE is a putative mitochondrial transporter involved in photorespiratory metabolism and is required for meristem growth at ambient CO2 levels
Visit the PubMed ArticleExpression of the GDCH1 ... encoding glycine decarboxylase subunit ... is down-regulated in bou-2
Eisenhut M, Planchais S, Cabassa C, Guivarc'h A, Justin AM, Taconnat L, Renou JP, Linka M, Gagneul D, Timm S, Bauwe H, Carol P, Weber AP - Arabidopsis A BOUT DE SOUFFLE is a putative mitochondrial transporter involved in photorespiratory metabolism and is required for meristem growth at ambient CO2 levels
Visit the PubMed ArticleCarbon assimilation genes such as ... Rubisco activase (RCA) ... down-regulated in bou-2
Eisenhut M, Planchais S, Cabassa C, Guivarc'h A, Justin AM, Taconnat L, Renou JP, Linka M, Gagneul D, Timm S, Bauwe H, Carol P, Weber AP - Arabidopsis A BOUT DE SOUFFLE is a putative mitochondrial transporter involved in photorespiratory metabolism and is required for meristem growth at ambient CO2 levels
Visit the PubMed ArticleBOU gene expression is light-inducible and dependent on photon flux density (Figure 1b–d and Figure S1a,b) (Lawand et al., 2002) and plastid signals
Eisenhut M, Planchais S, Cabassa C, Guivarc'h A, Justin AM, Taconnat L, Renou JP, Linka M, Gagneul D, Timm S, Bauwe H, Carol P, Weber AP - Arabidopsis A BOUT DE SOUFFLE is a putative mitochondrial transporter involved in photorespiratory metabolism and is required for meristem growth at ambient CO2 levels
Visit the PubMed ArticleWe performed protein gel-blot analyses with specific antibodies ... Levels of ... SHM1 were not influenced by loss of the BOU protein
Eisenhut M, Planchais S, Cabassa C, Guivarc'h A, Justin AM, Taconnat L, Renou JP, Linka M, Gagneul D, Timm S, Bauwe H, Carol P, Weber AP - Arabidopsis A BOUT DE SOUFFLE is a putative mitochondrial transporter involved in photorespiratory metabolism and is required for meristem growth at ambient CO2 levels
Visit the PubMed ArticleWe performed protein gel-blot analyses with specific antibodies ... Levels of ... GDT ... were not influenced by loss of the BOU protein
Eisenhut M, Planchais S, Cabassa C, Guivarc'h A, Justin AM, Taconnat L, Renou JP, Linka M, Gagneul D, Timm S, Bauwe H, Carol P, Weber AP - Arabidopsis A BOUT DE SOUFFLE is a putative mitochondrial transporter involved in photorespiratory metabolism and is required for meristem growth at ambient CO2 levels
Visit the PubMed ArticleBOU promoter activity was observed in cotyledons
Eisenhut M, Planchais S, Cabassa C, Guivarc'h A, Justin AM, Taconnat L, Renou JP, Linka M, Gagneul D, Timm S, Bauwe H, Carol P, Weber AP - Arabidopsis A BOUT DE SOUFFLE is a putative mitochondrial transporter involved in photorespiratory metabolism and is required for meristem growth at ambient CO2 levels
Visit the PubMed Articlein bou-2 ... chlorophyllase ATCHL2 (At5 g43860), the first enzyme in chlorophyll degradation (Tsuchiya et al., 1999), was up-regulated
Eisenhut M, Planchais S, Cabassa C, Guivarc'h A, Justin AM, Taconnat L, Renou JP, Linka M, Gagneul D, Timm S, Bauwe H, Carol P, Weber AP - Arabidopsis A BOUT DE SOUFFLE is a putative mitochondrial transporter involved in photorespiratory metabolism and is required for meristem growth at ambient CO2 levels
Visit the PubMed ArticleCarbon assimilation genes such as ... Rubisco small subunit ... RBSC3B ... down-regulated in bou-2
Eisenhut M, Planchais S, Cabassa C, Guivarc'h A, Justin AM, Taconnat L, Renou JP, Linka M, Gagneul D, Timm S, Bauwe H, Carol P, Weber AP - Arabidopsis A BOUT DE SOUFFLE is a putative mitochondrial transporter involved in photorespiratory metabolism and is required for meristem growth at ambient CO2 levels
Visit the PubMed ArticleCarbon assimilation genes such as ... Rubisco small subunit ... RBCS1A ... down-regulated in bou-2
Eisenhut M, Planchais S, Cabassa C, Guivarc'h A, Justin AM, Taconnat L, Renou JP, Linka M, Gagneul D, Timm S, Bauwe H, Carol P, Weber AP - Arabidopsis A BOUT DE SOUFFLE is a putative mitochondrial transporter involved in photorespiratory metabolism and is required for meristem growth at ambient CO2 levels
Visit the PubMed ArticleWe crossed the CycB1;1::uidA reporter gene, which is specifically expressed in the G2 and mitosis (M) phases of the cell cycle, into the WT and bou-2 backgrounds (Colón-Carmona et al., 1999). WT shoot apices were GUS-stained, revealing that numerous cells are in the G2 and M phases and thus dividing. bou-2 seedlings showed little or no GUS staining in the apical region, so cells were either at other phases of the cell cycle or had exited the cell cycle (Figure 6b). When supplemented with sucrose for 24 h, mutant seedlings displayed GUS staining in shoot apices ... The effect of 1% CO2 on cell division was also investigated. Scattered dense staining of both leaf primordia and root meristems of CycB1;1::uidA/bou-2 plants showed that cell division resumed 1 day after bou-2 was transferred to 1% CO2
Eisenhut M, Planchais S, Cabassa C, Guivarc'h A, Justin AM, Taconnat L, Renou JP, Linka M, Gagneul D, Timm S, Bauwe H, Carol P, Weber AP - Arabidopsis A BOUT DE SOUFFLE is a putative mitochondrial transporter involved in photorespiratory metabolism and is required for meristem growth at ambient CO2 levels
Visit the PubMed Articlein bou-2 ... an alanine glyoxylate:aminotransferase (AGT3) involved in conversion of alanine and glyoxylate to glycine ... up-regulated
Eisenhut M, Planchais S, Cabassa C, Guivarc'h A, Justin AM, Taconnat L, Renou JP, Linka M, Gagneul D, Timm S, Bauwe H, Carol P, Weber AP - Arabidopsis A BOUT DE SOUFFLE is a putative mitochondrial transporter involved in photorespiratory metabolism and is required for meristem growth at ambient CO2 levels
Visit the PubMed ArticleWe performed protein gel-blot analyses with specific antibodies ... by loss of the BOU protein ... distinct changes were found for GDP. In protein extracts from bou-2 grown at 1% CO2, the amount of GDP was somewhat elevated compared to WT. In bou-2 plants shifted to ambient air, we detected decreased amounts of GDP ... Intriguingly, an additional signal was detected using the GDP-specific antibody. This signal probably originated from a degradation product of GDP. This GDP fragment also occurred in WT (Figure S4), but accumulated to a much greater extent in bou-2 after the shift to ambient air
Eisenhut M, Planchais S, Cabassa C, Guivarc'h A, Justin AM, Taconnat L, Renou JP, Linka M, Gagneul D, Timm S, Bauwe H, Carol P, Weber AP - Arabidopsis A BOUT DE SOUFFLE is a putative mitochondrial transporter involved in photorespiratory metabolism and is required for meristem growth at ambient CO2 levels
Visit the PubMed ArticleLipoylation of GDH and the E2 subunits of pyruvate dehydrogenase was not affected in bou-2, but the amount of lipoylated E2 subunit of a-ketoacid dehydrogenase was increased in protein extracts from bou-2 plants after the shift to ambient air
Eisenhut M, Planchais S, Cabassa C, Guivarc'h A, Justin AM, Taconnat L, Renou JP, Linka M, Gagneul D, Timm S, Bauwe H, Carol P, Weber AP - Arabidopsis A BOUT DE SOUFFLE is a putative mitochondrial transporter involved in photorespiratory metabolism and is required for meristem growth at ambient CO2 levels
Visit the PubMed ArticleBOU promoter activity was observed in ... leaf tissues
Eisenhut M, Planchais S, Cabassa C, Guivarc'h A, Justin AM, Taconnat L, Renou JP, Linka M, Gagneul D, Timm S, Bauwe H, Carol P, Weber AP - Arabidopsis A BOUT DE SOUFFLE is a putative mitochondrial transporter involved in photorespiratory metabolism and is required for meristem growth at ambient CO2 levels
Visit the PubMed ArticleBOU gene is strongly co-expressed with the genes GDP2 (At2 g26080), GDP1 (At4 g33010), GDT1 (At1 g11860) and GDH3 (At1 g32470), encoding subunits of GDC, and SHM1 (At4 g37930), encoding the mitochondrial serine hydroxymethyl transferase SHM1
Eisenhut M, Planchais S, Cabassa C, Guivarc'h A, Justin AM, Taconnat L, Renou JP, Linka M, Gagneul D, Timm S, Bauwe H, Carol P, Weber AP - Arabidopsis A BOUT DE SOUFFLE is a putative mitochondrial transporter involved in photorespiratory metabolism and is required for meristem growth at ambient CO2 levels
Visit the PubMed Articlein bou-2 ... pyruvate and a threonine aldolase (THA1) involved in the synthesis of glycine from threonine ... up-regulated
Eisenhut M, Planchais S, Cabassa C, Guivarc'h A, Justin AM, Taconnat L, Renou JP, Linka M, Gagneul D, Timm S, Bauwe H, Carol P, Weber AP - Arabidopsis A BOUT DE SOUFFLE is a putative mitochondrial transporter involved in photorespiratory metabolism and is required for meristem growth at ambient CO2 levels
Visit the PubMed ArticleBOU promoter activity ... appeared to be absent from meristematic tissues
Eisenhut M, Planchais S, Cabassa C, Guivarc'h A, Justin AM, Taconnat L, Renou JP, Linka M, Gagneul D, Timm S, Bauwe H, Carol P, Weber AP - Arabidopsis A BOUT DE SOUFFLE is a putative mitochondrial transporter involved in photorespiratory metabolism and is required for meristem growth at ambient CO2 levels
Visit the PubMed ArticleHIS4 ... strongly repressed in bou-2
Eisenhut M, Planchais S, Cabassa C, Guivarc'h A, Justin AM, Taconnat L, Renou JP, Linka M, Gagneul D, Timm S, Bauwe H, Carol P, Weber AP - Arabidopsis A BOUT DE SOUFFLE is a putative mitochondrial transporter involved in photorespiratory metabolism and is required for meristem growth at ambient CO2 levels
Visit the PubMed Articlea linker histone gene, HIS1-3, was expressed at a higher level in bou-2
Eisenhut M, Planchais S, Cabassa C, Guivarc'h A, Justin AM, Taconnat L, Renou JP, Linka M, Gagneul D, Timm S, Bauwe H, Carol P, Weber AP - Arabidopsis A BOUT DE SOUFFLE is a putative mitochondrial transporter involved in photorespiratory metabolism and is required for meristem growth at ambient CO2 levels
Visit the PubMed ArticleExpression of an STM promoter–GUS reporter gene was always detected in the bou-2 mutant (Figure S6), suggesting that meristem cell identity is somehow maintained despite the strong meristem disorganization and low cell-cycle activity
Eisenhut M, Planchais S, Cabassa C, Guivarc'h A, Justin AM, Taconnat L, Renou JP, Linka M, Gagneul D, Timm S, Bauwe H, Carol P, Weber AP - Arabidopsis A BOUT DE SOUFFLE is a putative mitochondrial transporter involved in photorespiratory metabolism and is required for meristem growth at ambient CO2 levels
Visit the PubMed ArticleWe performed protein gel-blot analyses with specific antibodies ... Levels of GDH ... were not influenced by loss of the BOU protein
Eisenhut M, Planchais S, Cabassa C, Guivarc'h A, Justin AM, Taconnat L, Renou JP, Linka M, Gagneul D, Timm S, Bauwe H, Carol P, Weber AP - Arabidopsis A BOUT DE SOUFFLE is a putative mitochondrial transporter involved in photorespiratory metabolism and is required for meristem growth at ambient CO2 levels
Visit the PubMed ArticleExpression of the ... GDCT ... encoding glycine decarboxylase subunit ... is down-regulated in bou-2
Eisenhut M, Planchais S, Cabassa C, Guivarc'h A, Justin AM, Taconnat L, Renou JP, Linka M, Gagneul D, Timm S, Bauwe H, Carol P, Weber AP - Arabidopsis A BOUT DE SOUFFLE is a putative mitochondrial transporter involved in photorespiratory metabolism and is required for meristem growth at ambient CO2 levels
Visit the PubMed Articlein rh3–4 ... Expression of ... NCED4 (9–cis–epoxycarotenoid dehydrogenase 4), a member of the dehydrogenase family that cleaves 9–cis–carotenoids to xanthoxin ... was suppressed
Lee KH, Park J, Williams DS, Xiong Y, Hwang I, Kang BH - Defective chloroplast development inhibits maintenance of normal levels of abscisic acid in a mutant of the Arabidopsis RH3 DEAD-box protein during early post-germination growth
Visit the PubMed ArticleTo examine the tissue-specific expression patterns of RH3, we generated transgenic plants expressing a transcriptional fusion construct with the RH3 promoter (2.0 kb) and the ß–glucuronidase (GUS) reporter gene. T2 progeny from three independent transgenic RH3p-GUS lines were used for GUS activity analysis (Figure 1b–f). GUS staining was strongest in the cotyledons and hypocotyls of germinating seedlings (Figure 1b). The GUS activity became weak in the cotyledons and hypocotyls in 1-week-old seedlings, and decreased further in true leaves in older seedlings
Lee KH, Park J, Williams DS, Xiong Y, Hwang I, Kang BH - Defective chloroplast development inhibits maintenance of normal levels of abscisic acid in a mutant of the Arabidopsis RH3 DEAD-box protein during early post-germination growth
Visit the PubMed ArticleTo further examine chloroplast ribosome biogenesis in the rh3–4 mutant, we performed RNA blot analysis using probes that recognize chloroplast rRNA molecules (Figure 6a). Consistent with the electron tomography imaging results (Figure 5), which showed a decrease in chloroplast ribosome density, the amounts of chloroplast rRNA were significantly reduced. We did not detect unusual forms of chloroplast rRNA in total RNA samples from the 3-day-old rh3–4 mutant when equal amounts of samples were analyzed
Lee KH, Park J, Williams DS, Xiong Y, Hwang I, Kang BH - Defective chloroplast development inhibits maintenance of normal levels of abscisic acid in a mutant of the Arabidopsis RH3 DEAD-box protein during early post-germination growth
Visit the PubMed ArticleTo determine the subcellular localization of RH3, we performed immunoelectron microscopy with transgenic Arabidopsis plants expressing an RH3::FLAG fusion protein under the control of its native promoter. FLAG-specific immunogold particles were observed mostly in the chloroplast stroma of the transgenic cotyledon cells
Lee KH, Park J, Williams DS, Xiong Y, Hwang I, Kang BH - Defective chloroplast development inhibits maintenance of normal levels of abscisic acid in a mutant of the Arabidopsis RH3 DEAD-box protein during early post-germination growth
Visit the PubMed Articlein rh3–4 ... Expression of ABA1, the first enzyme of the pathway ... was suppressed
Lee KH, Park J, Williams DS, Xiong Y, Hwang I, Kang BH - Defective chloroplast development inhibits maintenance of normal levels of abscisic acid in a mutant of the Arabidopsis RH3 DEAD-box protein during early post-germination growth
Visit the PubMed Articletranscriptional fusion construct with the RH3 promoter (2.0 kb) and the ß–glucuronidase (GUS) reporter gene ... GUS expression was detected in the apical zone of the first pair of true leaves
Lee KH, Park J, Williams DS, Xiong Y, Hwang I, Kang BH - Defective chloroplast development inhibits maintenance of normal levels of abscisic acid in a mutant of the Arabidopsis RH3 DEAD-box protein during early post-germination growth
Visit the PubMed Articleaerial parts of wild-type and cpk28-1 plants were harvested at various developmental stages (10, 20, 27, 31 and 35 dag) and transcriptional changes of NST genes were monitored by qRT-PCR. Gene expression of ... NST3 ... enhanced in cpk28-1 mutant plants compared with the wild type ... Whereas transcription of NST1 was detectable in all five plant stages analysed, transcription of NST3 could not be detected at every age. In comparison with wild-type plants, where NST3 was only transcribed from 27 dag onwards, transcripts in cpk28-1 could be detected earlier (20 dag
Matschi S, Werner S, Schulze WX, Legen J, Hilger HH, Romeis T - Function of calcium-dependent protein kinase CPK28 of Arabidopsis thaliana in plant stem elongation and vascular development
Visit the PubMed ArticleCPK28 (pCPK28::GUS) ... promoter activity appeared to be associated with the ... the rosette of older plants
Matschi S, Werner S, Schulze WX, Legen J, Hilger HH, Romeis T - Function of calcium-dependent protein kinase CPK28 of Arabidopsis thaliana in plant stem elongation and vascular development
Visit the PubMed Articleaerial parts of wild-type and cpk28-1 plants were harvested at various developmental stages (10, 20, 27, 31 and 35 dag) and transcriptional changes of NST genes were monitored by qRT-PCR. Gene expression of NST1 ... enhanced in cpk28-1 mutant plants compared with the wild type
Matschi S, Werner S, Schulze WX, Legen J, Hilger HH, Romeis T - Function of calcium-dependent protein kinase CPK28 of Arabidopsis thaliana in plant stem elongation and vascular development
Visit the PubMed ArticleTo investigate tissue- and cell type-specific expression of CPK28 during development of A. thaliana, transgenic lines expressing the uidA (ß-glucuronidase – GUS) gene under the control of a 1.8 kb upstream promoter sequence from CPK28 (pCPK28::GUS) were generated. GUS activity was detected throughout various plant stages in different tissues, mainly in the vasculature and in meristematic regions (Figure 4). GUS activity was evident in the vasculature of seedlings, of older plants and of mature rosette leaves
Matschi S, Werner S, Schulze WX, Legen J, Hilger HH, Romeis T - Function of calcium-dependent protein kinase CPK28 of Arabidopsis thaliana in plant stem elongation and vascular development
Visit the PubMed Articleat the transition to generative phase-dependent shoot elongation in the wild-type, gene expression of GA3ox1 dropped in cpk28 mutant plants (31 and 35 dag). The same transcriptional down-regulation of GA3ox1 was confirmed for the second allele cpk28-2 at 31 dag
Matschi S, Werner S, Schulze WX, Legen J, Hilger HH, Romeis T - Function of calcium-dependent protein kinase CPK28 of Arabidopsis thaliana in plant stem elongation and vascular development
Visit the PubMed Articlein cpk28 mutant lines ... GA treatment, sufficient to trigger a partial reversal of the cpk28 mutant stem elongation phenotype, resulted in even higher NST1 ... transcript quantities
Matschi S, Werner S, Schulze WX, Legen J, Hilger HH, Romeis T - Function of calcium-dependent protein kinase CPK28 of Arabidopsis thaliana in plant stem elongation and vascular development
Visit the PubMed ArticleReduced expression of GA marker gene GA3ox1 was observed in cpk28 plants (Figure 6), and exogenous GA resulted in an even greater reduction of transcript amount
Matschi S, Werner S, Schulze WX, Legen J, Hilger HH, Romeis T - Function of calcium-dependent protein kinase CPK28 of Arabidopsis thaliana in plant stem elongation and vascular development
Visit the PubMed Articleaerial parts of wild-type and cpk28-1 plants were harvested at various developmental stages (10, 20, 27, 31 and 35 dag) and transcriptional changes of NST genes were monitored by qRT-PCR ... the expression of transcription factor NST2, for which no function in stem secondary growth has been reported (Zhong et al., 2008), remained unaltered
Matschi S, Werner S, Schulze WX, Legen J, Hilger HH, Romeis T - Function of calcium-dependent protein kinase CPK28 of Arabidopsis thaliana in plant stem elongation and vascular development
Visit the PubMed Articlein cpk28 mutant lines ... GA treatment, sufficient to trigger a partial reversal of the cpk28 mutant stem elongation phenotype, resulted in even higher ... NST3 transcript quantities
Matschi S, Werner S, Schulze WX, Legen J, Hilger HH, Romeis T - Function of calcium-dependent protein kinase CPK28 of Arabidopsis thaliana in plant stem elongation and vascular development
Visit the PubMed Article2010). Gene expression was analysed at various developmental stages (10, 20, 27, 31 and 35 dag) of wild-type and cpk28-1 by qRT-PCR (Figure 6e,f). No differences in ... GA3ox1 transcript levels were detected during vegetative growth (up to 27 dag
Matschi S, Werner S, Schulze WX, Legen J, Hilger HH, Romeis T - Function of calcium-dependent protein kinase CPK28 of Arabidopsis thaliana in plant stem elongation and vascular development
Visit the PubMed Article2010). Gene expression was analysed at various developmental stages (10, 20, 27, 31 and 35 dag) of wild-type and cpk28-1 by qRT-PCR (Figure 6e,f). No differences in GA20ox1 ... transcript levels were detected during vegetative growth (up to 27 dag)
Matschi S, Werner S, Schulze WX, Legen J, Hilger HH, Romeis T - Function of calcium-dependent protein kinase CPK28 of Arabidopsis thaliana in plant stem elongation and vascular development
Visit the PubMed ArticleCPK28 (pCPK28::GUS) ... promoter activity appeared to be associated with the meristematic zone of seedlings
Matschi S, Werner S, Schulze WX, Legen J, Hilger HH, Romeis T - Function of calcium-dependent protein kinase CPK28 of Arabidopsis thaliana in plant stem elongation and vascular development
Visit the PubMed ArticleCPK28 (pCPK28::GUS) ... In sections of the petiole of a fully expanded rosette leaf, GUS activity was seen in the vasculature
Matschi S, Werner S, Schulze WX, Legen J, Hilger HH, Romeis T - Function of calcium-dependent protein kinase CPK28 of Arabidopsis thaliana in plant stem elongation and vascular development
Visit the PubMed Articlequantitative reverse transcription polymerase chain reaction ... indicated that a member of the NAC transcription factor family, NST1 (NAC SECONDARY WALL THICKENING PROMOTING FACTOR1), showed higher expression levels in cpk28 mutant plants
Matschi S, Werner S, Schulze WX, Legen J, Hilger HH, Romeis T - Function of calcium-dependent protein kinase CPK28 of Arabidopsis thaliana in plant stem elongation and vascular development
Visit the PubMed ArticleWe next investigated whether the expression level of proteasome genes is translated into a change in proteasome activity. After 8 h of RPX induction, the protein level of PBE1 ... was increased as determined by Western blotting
Nguyen HM, Schippers JH, Gõni-Ramos O, Christoph MP, Dortay H, van der Hoorn RA, Mueller-Roeber B - An upstream regulator of the 26S proteasome modulates organ size in Arabidopsis thaliana
Visit the PubMed ArticleWe found that RPX expression is mainly restricted to the vascular tissue throughout the plant (Figure S3), in line with previous reports (Morishita et al., 2009). Transformation of tobacco with an Arabidopsis RPX promoter::GUS construct resulted in a similar expression pattern in leaves as observed for Arabidopsis (Figure S3g), indicating conserved upstream regulation of RPX
Nguyen HM, Schippers JH, Gõni-Ramos O, Christoph MP, Dortay H, van der Hoorn RA, Mueller-Roeber B - An upstream regulator of the 26S proteasome modulates organ size in Arabidopsis thaliana
Visit the PubMed ArticleBoth full-length and truncated RPX fused to GFP showed prominent nuclear localization
Nguyen HM, Schippers JH, Gõni-Ramos O, Christoph MP, Dortay H, van der Hoorn RA, Mueller-Roeber B - An upstream regulator of the 26S proteasome modulates organ size in Arabidopsis thaliana
Visit the PubMed ArticleRPX expression is induced during transition from the expansion phase to the maturation phase
Nguyen HM, Schippers JH, Gõni-Ramos O, Christoph MP, Dortay H, van der Hoorn RA, Mueller-Roeber B - An upstream regulator of the 26S proteasome modulates organ size in Arabidopsis thaliana
Visit the PubMed Articlewe performed microarray experiments using a ß–estradiol-inducible RPXΔC construct ... RPX induced the expression of six other TFs, including three uncharacterized NAC TFs ... ANAC094
Nguyen HM, Schippers JH, Gõni-Ramos O, Christoph MP, Dortay H, van der Hoorn RA, Mueller-Roeber B - An upstream regulator of the 26S proteasome modulates organ size in Arabidopsis thaliana
Visit the PubMed ArticleWe next investigated whether the expression level of proteasome genes is translated into a change in proteasome activity. After 8 h of RPX induction, the protein level of ... PBA1 ... was increased as determined by Western blotting
Nguyen HM, Schippers JH, Gõni-Ramos O, Christoph MP, Dortay H, van der Hoorn RA, Mueller-Roeber B - An upstream regulator of the 26S proteasome modulates organ size in Arabidopsis thaliana
Visit the PubMed ArticleArabidopsis contains 55 genes encoding subunits of the 26S proteasome and several putative UMP1-like maturation factors (Table S2). Expression of these genes was examined after 60, 90 and 120 min of induction of RPX, as well as in the over-expression and knockout lines, by quantitative real-time PCR (Figure 4 and Table S3). We found that 42 of the 55 genes tested were more than twofold induced after 120 min of ß–estradiol induction. In full-length over-expression plants, 24 genes were more highly expressed than in controls, while 46 genes were up-regulated in oxΔC plants, although the level of transgene expression for full-length and truncated RPX was comparable
Nguyen HM, Schippers JH, Gõni-Ramos O, Christoph MP, Dortay H, van der Hoorn RA, Mueller-Roeber B - An upstream regulator of the 26S proteasome modulates organ size in Arabidopsis thaliana
Visit the PubMed ArticleRPX is induced within 60 min of UV–B exposure and returns to basal levels after 12 h
Nguyen HM, Schippers JH, Gõni-Ramos O, Christoph MP, Dortay H, van der Hoorn RA, Mueller-Roeber B - An upstream regulator of the 26S proteasome modulates organ size in Arabidopsis thaliana
Visit the PubMed ArticleWe next investigated whether the expression level of proteasome genes is translated into a change in proteasome activity. After 8 h of RPX induction, the protein level of ... RPN10 was increased as determined by Western blotting
Nguyen HM, Schippers JH, Gõni-Ramos O, Christoph MP, Dortay H, van der Hoorn RA, Mueller-Roeber B - An upstream regulator of the 26S proteasome modulates organ size in Arabidopsis thaliana
Visit the PubMed Articlewe performed microarray experiments using a ß–estradiol-inducible RPXΔC construct ... RPX induced the expression of six other TFs, including three uncharacterized NAC TFs ... ANAC103
Nguyen HM, Schippers JH, Gõni-Ramos O, Christoph MP, Dortay H, van der Hoorn RA, Mueller-Roeber B - An upstream regulator of the 26S proteasome modulates organ size in Arabidopsis thaliana
Visit the PubMed ArticleBoth full-length and truncated RPX fused to GFP showed ... weak cytoplasmic localization was also observed in some cases
Nguyen HM, Schippers JH, Gõni-Ramos O, Christoph MP, Dortay H, van der Hoorn RA, Mueller-Roeber B - An upstream regulator of the 26S proteasome modulates organ size in Arabidopsis thaliana
Visit the PubMed Articlewe performed microarray experiments using a ß–estradiol-inducible RPXΔC construct ... RPX induced the expression of six other TFs, including three uncharacterized NAC TFs ... ANAC087
Nguyen HM, Schippers JH, Gõni-Ramos O, Christoph MP, Dortay H, van der Hoorn RA, Mueller-Roeber B - An upstream regulator of the 26S proteasome modulates organ size in Arabidopsis thaliana
Visit the PubMed Articleunspliced pri-miRNA172a was accumulated more in sta1-1 than in Col-0
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed ArticleThe accumulation levels of ... miR393 ... were reduced in sta1-1 compared with WT
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed ArticleIntriguingly, the intron-less pri-miR160b transcripts were accumulated slightly more in sta1-1 than in Col-0, which was similar to those in se-1 and hyl1-2
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed ArticleTo investigate the involvement of STA1 in the pri-miRNA processing, we determined pri-miRNA transcript levels by qRT-PCR (Figure 4). Transcripts of all tested pri-miRNAs accumulated more in sta1-1 compared with WT
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed ArticleHigh levels of unspliced pri-miRNA166a transcripts accumulated in se-1, hyl1-2 and sta1-1 as compared with Col-0, indicating the similar impairment in pri-miR166a splicing process in these mutants
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed ArticleThe unspliced pri-miR160a (~2.0 kb) accumulated more in se-1 and sta1-1 compared with Col-0 and hyl1-2 (Figure 5A). The se-1 and hyl1-2 mutants displayed a band ~850 bp which corresponds to the predicted size of mature pri-miR160a, while both Col-0 and sta1-1 did not show this band
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed ArticleCompared with WT, the accumulation of CUC1 ... transcripts was higher in sta1-1, which is linked to decreased miR164/166 levels and explains the serrated leaf phenotype of sta1-1
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed ArticleHigher accumulation of unspliced pri-miR172b was also observed in sta1-1 compared with Col-0, se-1 and hyl1-2. In addition to the intron retention in sta1-1, we observed unusual accumulation of the predicted mature pri-miR172b (~0.7 kb), which was present in Col-0, se-1 and hyl1-2 at very similar levels (Figure 5E). Normally, mature pri-miRNAs rapidly undergo further processing to pre-miRNAs that are known to be less detectable in WT than the processing defective mutants (59). To clarify the identity of the ~0.7-kb fragment in Col-0, we cloned the fragment for sequence analysis and found that the fragment is a mis-spliced product caused by an exon-skipping at 5'-region during splicing
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed ArticleThe phenotypic resemblances among sta1-1, se-1, hyl1-2 and abh1/cbp80 led us to investigate the levels of miRNAs in sta1-1. Using the Illumina platform, we generated 20.4 and 28.0 million clean reads that perfectly matched the Arabidopsis genome from the small RNA populations in sta1-1 and WT, respectively. We compared the normalized counts of mature miRNAs in sta1-1 and WT and found that the majority of miRNAs had lower expression in sta1-1
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed ArticleAPETALA 2 (AP2), a target of miR172 (56), showed a slight increase in se-1, hyl1-2 and sta1-1 that accumulated lower amounts of miR172 than WT
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed ArticleThe accumulation levels of ... miR173 ... were reduced in sta1-1 compared with WT
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed ArticleHigh levels of unspliced pri-miRNA166a transcripts accumulated in se-1, hyl1-2 and sta1-1 as compared with Col-0, indicating the similar impairment in pri-miR166a splicing process in these mutants
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed Articlean increase of the SPL3 transcript level was observed in sta1-1
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed Articleseveral bands ranging from ~0.85 to ~1.4 kb accumulated to high levels in se-1 and hyl1-2, but not in Col-0 and sta1-1 (Figure 5E). Interestingly, the splicing intermediates of pri-miR172b were more variable in hyl1-2 than in se-1. Indeed, the sequence analysis of these fragments showed various intermediates in hyl1-2 and only one kind of intermediate in se-1
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed ArticleThe accumulation levels of ... miR171 ... were reduced in sta1-1 compared with WT
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed Articleseveral bands ranging from ~0.85 to ~1.4 kb accumulated to high levels in se-1 and hyl1-2, but not in Col-0 and sta1-1 (Figure 5E). Interestingly, the splicing intermediates of pri-miR172b were more variable in hyl1-2 than in se-1. Indeed, the sequence analysis of these fragments showed various intermediates in hyl1-2 and only one kind of intermediate in se-1
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed ArticleWe also quantitatively evaluate the retention of introns in pri-miR172b by comparing the levels of the introns in the WT control and sta1-1 using qRT-PCR. The retention levels of intron 1 and 2 in pri-miR172b were higher in se-1, hyl1-2 and sta1-1 than Col-0, while the retention of intron 3 was high only in sta1-1
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed ArticleCompared with WT, the accumulation of ... REV transcripts was higher in sta1-1, which is linked to decreased miR164/166 levels and explains the serrated leaf phenotype of sta1-1
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed ArticleHigh levels of unspliced pri-miRNA166a transcripts accumulated in se-1, hyl1-2 and sta1-1 as compared with Col-0, indicating the similar impairment in pri-miR166a splicing process in these mutants
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed ArticleThe accumulation levels of miR156 ... were reduced in sta1-1 compared with WT
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed ArticleIn contrast to a previous report (57), we could not observe the spliced fragments of pri-miR166 in hyl1-2. We suspect that the splicing efficiency of intron containing pri-miRNAs may be more dependent on growth conditions or developmental stages in hyl1-2 than other mutants
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed ArticleThe accumulation levels of ... miR447 were reduced in sta1-1 compared with WT
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed ArticleThe accumulation levels of ... miR158 ... were reduced in sta1-1 compared with WT
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed Articlethe expression of miR164 was ... in the sta1-1 ... slightly decreased in the small RNA blot hybridization
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed Articletranscript level of DCL1 is reduced in sta1-1
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed Articleunspliced pri-miR166b was accumulated more in ... hyl1-2
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed ArticleHigher levels of ARF17 transcripts accumulated in sta1-1, consistent with its reduced miR160 levels compared with WT
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed Articlesta1-1 with decreased miR159 levels showed marginally increased expression levels of MYB33
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed ArticleWe also quantitatively evaluate the retention of introns in pri-miR172b by comparing the levels of the introns in the WT control and sta1-1 using qRT-PCR. The retention levels of intron 1 and 2 in pri-miR172b were higher in ... hyl1-2 ... than Col-0
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed ArticleThe accumulation levels of ... miR157 ... were reduced in sta1-1 compared with WT
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed ArticleThe accumulation levels of ... miR172 ... were reduced in sta1-1 compared with WT
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed ArticleThe accumulation levels of ... miR166 ... were reduced in sta1-1 compared with WT
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed Articleunspliced pri-miR166b was accumulated more in ... sta1-1 than in Col-0
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed ArticleThe accumulation levels of ... miR159 ... were reduced in sta1-1 compared with WT
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed ArticleThe unspliced pri-miR160a (~2.0 kb) accumulated more in se-1 and sta1-1 compared with Col-0 and hyl1-2 (Figure 5A). The se-1 and hyl1-2 mutants displayed a band ~850 bp which corresponds to the predicted size of mature pri-miR160a, while both Col-0 and sta1-1 did not show this band
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed ArticleThe only difference between the small RNA blot hybridization and Illumina sequencing was the level of miR164; the expression of miR164 was slightly increased in the sta1-1 sequencing results
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed ArticleThe accumulation levels of ... miR398 ... were reduced in sta1-1 compared with WT
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed ArticleThe accumulation levels of ... miR168 ... were reduced in sta1-1 compared with WT
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed ArticleThe accumulation levels of ... miR160 ... were reduced in sta1-1 compared with WT
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed ArticleWe also quantitatively evaluate the retention of introns in pri-miR172b by comparing the levels of the introns in the WT control and sta1-1 using qRT-PCR. The retention levels of intron 1 and 2 in pri-miR172b were higher in se-1 ... than Col-0
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed Articleunspliced pri-miR166b was accumulated more in se-1 ... than in Col-0
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed ArticleThe accumulation levels of ... miR162 ... were reduced in sta1-1 compared with WT
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed ArticleThe unspliced pri-miR160a (~2.0 kb) accumulated more in se-1 and sta1-1 compared with Col-0 and hyl1-2 (Figure 5A). The se-1 and hyl1-2 mutants displayed a band ~850 bp which corresponds to the predicted size of mature pri-miR160a, while both Col-0 and sta1-1 did not show this band
Ben Chaabane S, Liu R, Chinnusamy V, Kwon Y, Park JH, Kim SY, Zhu JK, Yang SW, Lee BH. - STA1, an Arabidopsis pre-mRNA processing factor 6 homolog, is a new player involved in miRNA biogenesis
Visit the PubMed ArticleBased on qRT-PCR analysis, we found that expression of ... LAC13 ... are significantly repressed in the miR408-OX plants
Zhang H, Li L - SQUAMOSA promoter binding protein-like7 regulated microRNA408 is required for vegetative development in Arabidopsis
Visit the PubMed Articlesupplementing MS with 5 µm copper (MS + Cu) effectively prevents the seedlings from accumulating miR408
Zhang H, Li L - SQUAMOSA promoter binding protein-like7 regulated microRNA408 is required for vegetative development in Arabidopsis
Visit the PubMed ArticleT–DNA lines carrying insertions in the MIR408 locus ... FLAG_545E03 and ... FLAG_481E04 ... We found both mutants show increased ... LAC13 levels compared with the wild type
Zhang H, Li L - SQUAMOSA promoter binding protein-like7 regulated microRNA408 is required for vegetative development in Arabidopsis
Visit the PubMed ArticleWe designed an artificial microRNA, amiR408, driven by the 35S promoter to specifically target the pre-miR408 sequence ... As an indicator for reduced miR408 activity ... Plantacyanin transcripts were found at much increased levels in the amiR408 plants
Zhang H, Li L - SQUAMOSA promoter binding protein-like7 regulated microRNA408 is required for vegetative development in Arabidopsis
Visit the PubMed ArticleBased on qRT-PCR analysis, we found that expression of ... Plantacyanin are significantly repressed in the miR408-OX plants
Zhang H, Li L - SQUAMOSA promoter binding protein-like7 regulated microRNA408 is required for vegetative development in Arabidopsis
Visit the PubMed ArticleT–DNA lines carrying insertions in the MIR408 locus ... FLAG_545E03 and ... FLAG_481E04 ... We found both mutants show increased Plantacyanin ... levels compared with the wild type
Zhang H, Li L - SQUAMOSA promoter binding protein-like7 regulated microRNA408 is required for vegetative development in Arabidopsis
Visit the PubMed ArticleSimilar to mature miR408, qRT-PCR analysis showed that pre-miR408 is accumulated to a much higher level under low copper conditions
Zhang H, Li L - SQUAMOSA promoter binding protein-like7 regulated microRNA408 is required for vegetative development in Arabidopsis
Visit the PubMed ArticleT–DNA lines carrying insertions in the MIR408 locus ... Considering that At2 g47020, which encodes a peptide chain release factor, overlaps with the miR408 locus, we examined whether this gene is impacted when miR408 is silenced. As shown in Figure S3, using the primer pair flanking the T–DNA insertion sites, we found that the transcript level of At2 g47020 is reduced in three of the T–DNA lines, but increased in the other three when compared with the wild type. Using the primer pair downstream of all insertion sites, we found that the At2 g47020 level is increased in all T–DNA lines except FLAG_545E03. Thus, the T–DNA lines disrupting miR408 does affect the At2 g47020 locus; however, three critical pieces of evidence support the conclusion that the growth defects when knocking down miR408 are independent of the At2 g47020 gene
Zhang H, Li L - SQUAMOSA promoter binding protein-like7 regulated microRNA408 is required for vegetative development in Arabidopsis
Visit the PubMed Articlewe checked four individual amiR408 lines and found comparable expression levels of At2 g47020 with the wild type (Figure S4); thus, the amiR408 lines employed in this study are specific to miR408, and do not downregulate At2 g47020
Zhang H, Li L - SQUAMOSA promoter binding protein-like7 regulated microRNA408 is required for vegetative development in Arabidopsis
Visit the PubMed Articlewe analyzed the pMIR408:GUS transgenic plants in the wild-type background. When grown with copper supplement, GUS staining was visible in cotyledon veins, hypocotyl and the upper part of the root
Zhang H, Li L - SQUAMOSA promoter binding protein-like7 regulated microRNA408 is required for vegetative development in Arabidopsis
Visit the PubMed ArticleNorthern blot (Figure 5a) and qRT-PCR (Figure 5b) analyses confirmed that miR408 is present in young seedlings grown in standard MS media (with a copper concentration of 0.1 µm
Zhang H, Li L - SQUAMOSA promoter binding protein-like7 regulated microRNA408 is required for vegetative development in Arabidopsis
Visit the PubMed ArticleGUS activity indicates that the MIR408 promoter is active in ... cotyledon
Zhang H, Li L - SQUAMOSA promoter binding protein-like7 regulated microRNA408 is required for vegetative development in Arabidopsis
Visit the PubMed ArticleqRT-PCR analysis revealed that SPL7 is expressed at comparable or higher levels in adult organs
Zhang H, Li L - SQUAMOSA promoter binding protein-like7 regulated microRNA408 is required for vegetative development in Arabidopsis
Visit the PubMed Articlewe analyzed the pMIR408:GUS transgenic plants in the wild-type background ... strong GUS activity throughout the seedling was observed when the seedlings were grown on MS ... indicating that the MIR408 promoter is induced in low copper conditions. Together, our results demonstrate that transcriptional control is important for proper MIR408 expression in response to varying copper levels
Zhang H, Li L - SQUAMOSA promoter binding protein-like7 regulated microRNA408 is required for vegetative development in Arabidopsis
Visit the PubMed Articlewe introduced the pMIR408:GUS reporter gene in the spl7 background to generate the pMIR408:GUS/spl7 plants. Compared with wild-type seedlings expressing pMIR408:GUS (pMIR408:GUS/WT), pMIR408:GUS/spl7 seedlings have much reduced GUS staining with and without copper supplementation (Figure 5d), indicating that the activation of the MIR408 promoter under different copper conditions requires SPL7
Zhang H, Li L - SQUAMOSA promoter binding protein-like7 regulated microRNA408 is required for vegetative development in Arabidopsis
Visit the PubMed ArticleWe generated a reporter construct in which the ß–glucuronidase (GUS) gene is fused with the native promoter region, approximately 800 base pairs upstream of the TSS (pMIR408:GUS). We then generated transgenic Arabidopsis plants expressing this reporter construct and the vector control (Figure S1). When the transgenic plants were assayed, GUS activity indicates that the MIR408 promoter is active in both the green tissues (cotyledon, rosette and cauline leaf, sepal and silique coat) and vascular tissues (hypocotyl, root, vein and stem)
Zhang H, Li L - SQUAMOSA promoter binding protein-like7 regulated microRNA408 is required for vegetative development in Arabidopsis
Visit the PubMed ArticleNorthern blotting (Figure 5a) and qRT-PCR analyses (Figure 5b,c) showed that maintaining proper transcript levels as well as the induction of both miR408 and pre-miR408 in response to low copper are blocked by the spl7 mutation
Zhang H, Li L - SQUAMOSA promoter binding protein-like7 regulated microRNA408 is required for vegetative development in Arabidopsis
Visit the PubMed Articlewe first examined the tissue-specific expression profile of miR408 in Arabidopsis. By means of northern blotting and quantitative reverse transcription-coupled PCR (qRT-PCR), we found that mature miR408 is readily detectable in the seven organ types examined, including seedling, root, rosette and cauline leaves, stem, flower and silique
Zhang H, Li L - SQUAMOSA promoter binding protein-like7 regulated microRNA408 is required for vegetative development in Arabidopsis
Visit the PubMed ArticleWe designed an artificial microRNA, amiR408, driven by the 35S promoter to specifically target the pre-miR408 sequence ... As an indicator for reduced miR408 activity ... LAC13 ... transcripts were found at much increased levels in the amiR408 plants
Zhang H, Li L - SQUAMOSA promoter binding protein-like7 regulated microRNA408 is required for vegetative development in Arabidopsis
Visit the PubMed Articlea transgenic line harboring the pMUTE::GFP was crossed into aba2-2 mutant (Figure 4d,e). A scattered pattern of green fluorescent protein (GFP) expression was observed, correlated spatially with meristemoids and newly formed GMCs (Figure 4d). GFP fluorescence was detected in the cotyledon of the pMUTE::GFP line on day 3, but its expression could be hardly seen at later time points (Figure 4e). In the aba2-2 pMUTE::GFP line, the GFP signal was detected in meristemoids and GMCs similarly to that in the pMUTE::GFP line, but the aba2-2 pMUTE::GFP line contained more cells with the GFP signal and this signal could be observed later than the parental pMUTE::GFP line (Figure 4e). Collectively, these observations indicate that increased MUTE expression in the aba2-2 mutant is due to an increase in the number of cells that express MUTE
Tanaka Y, Nose T, Jikumaru Y, Kamiya Y - ABA inhibits entry into stomatal-lineage development in Arabidopsis leaves
Visit the PubMed ArticleExpression of ... MUTE in WT seedlings declined during this period, from day 3 to 9 ... the reduction of MUTE expression occurred gradually and its transcript was still detectable in WT on day 9 ... Transcripts of these genes were found to be more abundant in the aba2-2 mutant
Tanaka Y, Nose T, Jikumaru Y, Kamiya Y - ABA inhibits entry into stomatal-lineage development in Arabidopsis leaves
Visit the PubMed Articleincreased expression of ICK1, which encodes a cyclin-dependent kinase inhibitor protein that functions as a negative regulator of cell division and promoter of endoreduplication (Wang et al., 1998) in cyp707a1a3 compared with WT
Tanaka Y, Nose T, Jikumaru Y, Kamiya Y - ABA inhibits entry into stomatal-lineage development in Arabidopsis leaves
Visit the PubMed Articleincreased expression of CYCD3;1, a gene which encodes a cyclin D-type protein involved in the switch from cell proliferation to the final stages of mitotic cell cycle(Wang et al., 1998) in aba2-2
Tanaka Y, Nose T, Jikumaru Y, Kamiya Y - ABA inhibits entry into stomatal-lineage development in Arabidopsis leaves
Visit the PubMed ArticleThe abundance of ... MUTE gene transcription was also observed in ... abi1-1
Tanaka Y, Nose T, Jikumaru Y, Kamiya Y - ABA inhibits entry into stomatal-lineage development in Arabidopsis leaves
Visit the PubMed ArticleThe abundance of ... MUTE gene transcription was also observed in ... abi2-1
Tanaka Y, Nose T, Jikumaru Y, Kamiya Y - ABA inhibits entry into stomatal-lineage development in Arabidopsis leaves
Visit the PubMed ArticleMUTE expression ... reduced in cyp707a1a3 compared with WT
Tanaka Y, Nose T, Jikumaru Y, Kamiya Y - ABA inhibits entry into stomatal-lineage development in Arabidopsis leaves
Visit the PubMed ArticleThe abundance of SPCH ... gene transcription was also observed in ... abi1-1
Tanaka Y, Nose T, Jikumaru Y, Kamiya Y - ABA inhibits entry into stomatal-lineage development in Arabidopsis leaves
Visit the PubMed ArticleThe expression of SPCH ... reduced in cyp707a1a3 compared with WT
Tanaka Y, Nose T, Jikumaru Y, Kamiya Y - ABA inhibits entry into stomatal-lineage development in Arabidopsis leaves
Visit the PubMed ArticleExpression of ... SPCH ... in WT seedlings declined during this period, from day 3 to 9 (Figure 4c). The expression of SPCH declined steeply and was low on day 7 ... Transcripts of these genes were found to be more abundant in the aba2-2 mutant
Tanaka Y, Nose T, Jikumaru Y, Kamiya Y - ABA inhibits entry into stomatal-lineage development in Arabidopsis leaves
Visit the PubMed ArticleThe abundance of SPCH ... gene transcription was also observed in ... abi2-1
Tanaka Y, Nose T, Jikumaru Y, Kamiya Y - ABA inhibits entry into stomatal-lineage development in Arabidopsis leaves
Visit the PubMed ArticleYUC9 transcription was ... induced by oxylipins ... with a seven-fold increase in transcript levels after 120 min of OPDA treatment while MeJA and coronatine caused an even stronger induction (11- and 12-fold increase in transcript abundance 240-min post-treatment, respectively
Hentrich M, Böttcher C, Düchting P, Cheng Y, Zhao Y, Berkowitz O, Masle J, Medina J, Pollmann S - The jasmonic acid signaling pathway is linked to auxin homeostasis through the modulation of YUCCA8 and YUCCA9 gene expression
Visit the PubMed ArticleYUC8 ... transcript levels were significantly more abundant after short-term MeJA treatments ... yuc9
Hentrich M, Böttcher C, Düchting P, Cheng Y, Zhao Y, Berkowitz O, Masle J, Medina J, Pollmann S - The jasmonic acid signaling pathway is linked to auxin homeostasis through the modulation of YUCCA8 and YUCCA9 gene expression
Visit the PubMed ArticleYUC8 expression was highest in very young 3- and 7-day-old seedlings, thereafter declining. In adult 6–8-week-old plants, YUC8 transcript levels were highest in young sink leaves, lower in mature source leaves, roots and flower buds, and hardly detectable in stems
Hentrich M, Böttcher C, Düchting P, Cheng Y, Zhao Y, Berkowitz O, Masle J, Medina J, Pollmann S - The jasmonic acid signaling pathway is linked to auxin homeostasis through the modulation of YUCCA8 and YUCCA9 gene expression
Visit the PubMed ArticleYUC9 gene expression was generally one order of magnitude lower than YUC8 expression (Figure 3b). It was also developmentally regulated in young seedlings but with a peak 7 days after germination. In adult plants, YUC9 was predominantly expressed in roots and at a much lower level in sink leaves, while being undetectable in floral tissue
Hentrich M, Böttcher C, Düchting P, Cheng Y, Zhao Y, Berkowitz O, Masle J, Medina J, Pollmann S - The jasmonic acid signaling pathway is linked to auxin homeostasis through the modulation of YUCCA8 and YUCCA9 gene expression
Visit the PubMed ArticleYUC9 ... transcript levels were significantly more abundant after short-term MeJA treatments ... yuc8
Hentrich M, Böttcher C, Düchting P, Cheng Y, Zhao Y, Berkowitz O, Masle J, Medina J, Pollmann S - The jasmonic acid signaling pathway is linked to auxin homeostasis through the modulation of YUCCA8 and YUCCA9 gene expression
Visit the PubMed ArticleTo investigate whether oxylipin-dependent YUCCA expression is also governed by COI1, we treated the coi1 mutant with the various oxylipins and examined changes in ... YUC9 expression ... When compared with responses obtained 2 h after induction of wild-type plants, transcriptional responses to oxylipin treatment appeared almost entirely suppressed in the coi1 background
Hentrich M, Böttcher C, Düchting P, Cheng Y, Zhao Y, Berkowitz O, Masle J, Medina J, Pollmann S - The jasmonic acid signaling pathway is linked to auxin homeostasis through the modulation of YUCCA8 and YUCCA9 gene expression
Visit the PubMed ArticleTo investigate whether oxylipin-dependent YUCCA expression is also governed by COI1, we treated the coi1 mutant with the various oxylipins and examined changes in YUC8 ... expression ... When compared with responses obtained 2 h after induction of wild-type plants, transcriptional responses to oxylipin treatment appeared almost entirely suppressed in the coi1 background
Hentrich M, Böttcher C, Düchting P, Cheng Y, Zhao Y, Berkowitz O, Masle J, Medina J, Pollmann S - The jasmonic acid signaling pathway is linked to auxin homeostasis through the modulation of YUCCA8 and YUCCA9 gene expression
Visit the PubMed ArticleIn order to compare the subcellular localization of the encoded proteins, both genes were fused to the green fluorescent protein (GFP) reporter gene. The resulting constructs were then used to transiently transfect Arabidopsis pavement cells by ballistic gene transfer. Confocal laser scanning microscopy revealed an exclusive cytoplasmic localization of ... YUC9
Hentrich M, Böttcher C, Düchting P, Cheng Y, Zhao Y, Berkowitz O, Masle J, Medina J, Pollmann S - The jasmonic acid signaling pathway is linked to auxin homeostasis through the modulation of YUCCA8 and YUCCA9 gene expression
Visit the PubMed ArticleWe next generated ... pYUC9::GUS reporter lines ... GUS ... activity ... was ... confined to the very margin of sink leaves
Hentrich M, Böttcher C, Düchting P, Cheng Y, Zhao Y, Berkowitz O, Masle J, Medina J, Pollmann S - The jasmonic acid signaling pathway is linked to auxin homeostasis through the modulation of YUCCA8 and YUCCA9 gene expression
Visit the PubMed ArticleIn order to compare the subcellular localization of the encoded proteins, both genes were fused to the green fluorescent protein (GFP) reporter gene. The resulting constructs were then used to transiently transfect Arabidopsis pavement cells by ballistic gene transfer. Confocal laser scanning microscopy revealed an exclusive cytoplasmic localization of ... YUC8
Hentrich M, Böttcher C, Düchting P, Cheng Y, Zhao Y, Berkowitz O, Masle J, Medina J, Pollmann S - The jasmonic acid signaling pathway is linked to auxin homeostasis through the modulation of YUCCA8 and YUCCA9 gene expression
Visit the PubMed Articlewe measured YUC8 ... transcript abundance by qRT-PCR ... following application of MeJA, OPDA or coronatine, a bacteriotoxin that mimics the bioactive jasmonate JA-Ile ... In wild-type seedlings, all treatments resulted in a statistically significant reduction of YUC8 transcript levels within 30 min, followed by a progressive recovery within the next 3 h
Hentrich M, Böttcher C, Düchting P, Cheng Y, Zhao Y, Berkowitz O, Masle J, Medina J, Pollmann S - The jasmonic acid signaling pathway is linked to auxin homeostasis through the modulation of YUCCA8 and YUCCA9 gene expression
Visit the PubMed ArticleWe next generated pYUC8::GUS ... GUS reporter activity was absent from leaves
Hentrich M, Böttcher C, Düchting P, Cheng Y, Zhao Y, Berkowitz O, Masle J, Medina J, Pollmann S - The jasmonic acid signaling pathway is linked to auxin homeostasis through the modulation of YUCCA8 and YUCCA9 gene expression
Visit the PubMed ArticleAlso present are transcripts of several vascular patterning genes, including ... AGO1 ... which contain auxin-response elements (AuxREs) in their promoter regions
Gandotra N, Coughlan SJ, Nelson T - The Arabidopsis leaf provascular cell transcriptome is enriched in genes with roles in vein patterning
Visit the PubMed ArticleThe activity of the corresponding AtTZF9/AT5G58620 promoter is strong in the young and developing regions of the plant, with high activity in ... veins
Gandotra N, Coughlan SJ, Nelson T - The Arabidopsis leaf provascular cell transcriptome is enriched in genes with roles in vein patterning
Visit the PubMed ArticlePV/PC cells ... Also present are transcripts of several vascular patterning genes, including GNOM ... which contain auxin-response elements (AuxREs) in their promoter regions
Gandotra N, Coughlan SJ, Nelson T - The Arabidopsis leaf provascular cell transcriptome is enriched in genes with roles in vein patterning
Visit the PubMed ArticleThe ability to detect highly localized and transient expression is an advantage of cell-specific transcriptome analyses. To validate the accuracy of cell-specific transcriptome data, we observed the expression patterns of GUS reporter constructs made from representative genes from the PV/PC cell transcriptomes (Table S1). We confirmed that expression began in the PV/PC stage and extended into differentiating veins for ... AT2G21200 (putative auxin-responsive protein)
Gandotra N, Coughlan SJ, Nelson T - The Arabidopsis leaf provascular cell transcriptome is enriched in genes with roles in vein patterning
Visit the PubMed Articleexpression of ARF7 ... in PV/PC cells
Gandotra N, Coughlan SJ, Nelson T - The Arabidopsis leaf provascular cell transcriptome is enriched in genes with roles in vein patterning
Visit the PubMed ArticlePreviously characterized genes that are represented exclusively in the PV/PC cell transcriptome included ... VEP1
Gandotra N, Coughlan SJ, Nelson T - The Arabidopsis leaf provascular cell transcriptome is enriched in genes with roles in vein patterning
Visit the PubMed ArticleMP ... prominent in the PV/PC transcriptome
Gandotra N, Coughlan SJ, Nelson T - The Arabidopsis leaf provascular cell transcriptome is enriched in genes with roles in vein patterning
Visit the PubMed ArticleARF4 ... prominent in the PV/PC transcriptome
Gandotra N, Coughlan SJ, Nelson T - The Arabidopsis leaf provascular cell transcriptome is enriched in genes with roles in vein patterning
Visit the PubMed Articleexpression is detected only in the vascular cells from a mature and expanding leaf included APL
Gandotra N, Coughlan SJ, Nelson T - The Arabidopsis leaf provascular cell transcriptome is enriched in genes with roles in vein patterning
Visit the PubMed ArticleThe activity of the corresponding AtTZF9/AT5G58620 promoter ... Some weak expression was also seen in the epidermal cells
Gandotra N, Coughlan SJ, Nelson T - The Arabidopsis leaf provascular cell transcriptome is enriched in genes with roles in vein patterning
Visit the PubMed ArticlePreviously characterized genes that are represented exclusively in the PV/PC cell transcriptome included ... CVP1
Gandotra N, Coughlan SJ, Nelson T - The Arabidopsis leaf provascular cell transcriptome is enriched in genes with roles in vein patterning
Visit the PubMed ArticleAlso present are transcripts of several vascular patterning genes, including ... SFC ... which contain auxin-response elements (AuxREs) in their promoter regions
Gandotra N, Coughlan SJ, Nelson T - The Arabidopsis leaf provascular cell transcriptome is enriched in genes with roles in vein patterning
Visit the PubMed Articleexpression ... beginning in PV/PC cells and continuing into the differentiating vascular cells included MP
Gandotra N, Coughlan SJ, Nelson T - The Arabidopsis leaf provascular cell transcriptome is enriched in genes with roles in vein patterning
Visit the PubMed ArticleTo validate the accuracy of cell-specific transcriptome data, we observed the expression patterns of GUS reporter constructs made from representative genes from the PV/PC cell transcriptomes (Table S1). We confirmed that expression began in the PV/PC stage and extended into differentiating veins for ... AT1G76520 (annotated as an auxin efflux protein)
Gandotra N, Coughlan SJ, Nelson T - The Arabidopsis leaf provascular cell transcriptome is enriched in genes with roles in vein patterning
Visit the PubMed ArticleTo confirm the expression of KEG in PV/PC cells by an alternative method, we generated a KEG promoter:GUS reporter fusion construct and evaluated its expression pattern in transformed wild-type (WT/Col) plants. The KEG promoter ... was gradually limited in activity to the PV/PC cells at stages P3 and P4, and to the veins of later leaf stages
Gandotra N, Coughlan SJ, Nelson T - The Arabidopsis leaf provascular cell transcriptome is enriched in genes with roles in vein patterning
Visit the PubMed ArticleAlso present are transcripts of several vascular patterning genes, including ... PIN1 ... which contain auxin-response elements (AuxREs) in their promoter regions
Gandotra N, Coughlan SJ, Nelson T - The Arabidopsis leaf provascular cell transcriptome is enriched in genes with roles in vein patterning
Visit the PubMed Articleexpression of ... ARF5 ... in PV/PC cells
Gandotra N, Coughlan SJ, Nelson T - The Arabidopsis leaf provascular cell transcriptome is enriched in genes with roles in vein patterning
Visit the PubMed Articleexpression is detected only in the vascular cells from a mature and expanding leaf included ... IRX1
Gandotra N, Coughlan SJ, Nelson T - The Arabidopsis leaf provascular cell transcriptome is enriched in genes with roles in vein patterning
Visit the PubMed ArticlePreviously characterized genes that are represented exclusively in the PV/PC cell transcriptome included ... BRL1
Gandotra N, Coughlan SJ, Nelson T - The Arabidopsis leaf provascular cell transcriptome is enriched in genes with roles in vein patterning
Visit the PubMed ArticleThe ability to detect highly localized and transient expression is an advantage of cell-specific transcriptome analyses. To validate the accuracy of cell-specific transcriptome data, we observed the expression patterns of GUS reporter constructs made from representative genes from the PV/PC cell transcriptomes (Table S1). We confirmed that expression began in the PV/PC stage and extended into differentiating veins for ... AT1G32940 (ATSBT3.5; similar to subtilase family protein)
Gandotra N, Coughlan SJ, Nelson T - The Arabidopsis leaf provascular cell transcriptome is enriched in genes with roles in vein patterning
Visit the PubMed ArticleTo validate the accuracy of cell-specific transcriptome data, we observed the expression patterns of GUS reporter constructs made from representative genes from the PV/PC cell transcriptomes (Table S1). We confirmed that expression began in the PV/PC stage and extended into differentiating veins for AT1G15340 (MBD10 methylCpG binding domain containing protein)
Gandotra N, Coughlan SJ, Nelson T - The Arabidopsis leaf provascular cell transcriptome is enriched in genes with roles in vein patterning
Visit the PubMed Articleexpression ... beginning in PV/PC cells and continuing into the differentiating vascular cells included ... PINHEAD/ZWILLE/AGO10
Gandotra N, Coughlan SJ, Nelson T - The Arabidopsis leaf provascular cell transcriptome is enriched in genes with roles in vein patterning
Visit the PubMed ArticlePreviously characterized genes that are represented exclusively in the PV/PC cell transcriptome included ... AGD1
Gandotra N, Coughlan SJ, Nelson T - The Arabidopsis leaf provascular cell transcriptome is enriched in genes with roles in vein patterning
Visit the PubMed ArticleTo confirm the expression of KEG in PV/PC cells by an alternative method, we generated a KEG promoter:GUS reporter fusion construct and evaluated its expression pattern in transformed wild-type (WT/Col) plants. The KEG promoter was active throughout the emerging primordia (stages P1 and P2 ... leaf stages
Gandotra N, Coughlan SJ, Nelson T - The Arabidopsis leaf provascular cell transcriptome is enriched in genes with roles in vein patterning
Visit the PubMed Articleexpression ... beginning in PV/PC cells and continuing into the differentiating vascular cells included ... PINOID
Gandotra N, Coughlan SJ, Nelson T - The Arabidopsis leaf provascular cell transcriptome is enriched in genes with roles in vein patterning
Visit the PubMed Articleexpression is detected only in the vascular cells from a mature and expanding leaf included ... XYLOGEN
Gandotra N, Coughlan SJ, Nelson T - The Arabidopsis leaf provascular cell transcriptome is enriched in genes with roles in vein patterning
Visit the PubMed ArticleTo validate the accuracy of cell-specific transcriptome data, we observed the expression patterns of GUS reporter constructs made from representative genes from the PV/PC cell transcriptomes (Table S1). We confirmed that expression began in the PV/PC stage and extended into differentiating veins for ... AT2G26290 (ARSK1; protein kinase)
Gandotra N, Coughlan SJ, Nelson T - The Arabidopsis leaf provascular cell transcriptome is enriched in genes with roles in vein patterning
Visit the PubMed Articleexpression of ... IAA1 ... PV/PC cells
Gandotra N, Coughlan SJ, Nelson T - The Arabidopsis leaf provascular cell transcriptome is enriched in genes with roles in vein patterning
Visit the PubMed ArticlePreviously characterized genes that are represented exclusively in the PV/PC cell transcriptome included ... AGD4
Gandotra N, Coughlan SJ, Nelson T - The Arabidopsis leaf provascular cell transcriptome is enriched in genes with roles in vein patterning
Visit the PubMed Articleexpression ... beginning in PV/PC cells and continuing into the differentiating vascular cells included ... PARL1
Gandotra N, Coughlan SJ, Nelson T - The Arabidopsis leaf provascular cell transcriptome is enriched in genes with roles in vein patterning
Visit the PubMed Articleexpression of ... IAA19 in PV/PC cells
Gandotra N, Coughlan SJ, Nelson T - The Arabidopsis leaf provascular cell transcriptome is enriched in genes with roles in vein patterning
Visit the PubMed Articleexpression ... beginning in PV/PC cells and continuing into the differentiating vascular cells included ... IRX5
Gandotra N, Coughlan SJ, Nelson T - The Arabidopsis leaf provascular cell transcriptome is enriched in genes with roles in vein patterning
Visit the PubMed ArticleWe find that inositol polyphosphate 3-/6-/5- kinase (IPK2ß), which participates in the synthesis of IP6, is PV/PC-specific, in agreement with previous reports of its vascular expression (Zhang et al., 2007; Xia et al., 2003)
Gandotra N, Coughlan SJ, Nelson T - The Arabidopsis leaf provascular cell transcriptome is enriched in genes with roles in vein patterning
Visit the PubMed ArticleThe activity of the corresponding AtTZF9/AT5G58620 promoter is strong in the young and developing regions of the plant, with high activity in trichomes
Gandotra N, Coughlan SJ, Nelson T - The Arabidopsis leaf provascular cell transcriptome is enriched in genes with roles in vein patterning
Visit the PubMed ArticlePreviously characterized genes that are represented exclusively in the PV/PC cell transcriptome included ACL5
Gandotra N, Coughlan SJ, Nelson T - The Arabidopsis leaf provascular cell transcriptome is enriched in genes with roles in vein patterning
Visit the PubMed ArticleThe expression of AT2G26290 (ARSK1) has previously been reported to be specific to the roots (Hwang and Goodman, 1995) (and eFP browser http://bbc.botany.utoronto.ca/efp/cgi-bin/efpWeb.cgi). Since transcriptome data revealed this gene to be expressed in both early-stage and mature vascular cells of leaves, we tested the activity pattern in leaves of a reporter construct that included a longer promoter than previously published (3.035 kb from the 5' upstream region of ARSK1 versus 2.3 kb from the downstream region). With the longer upstream region, we found the previously reported root expression pattern, plus dynamic expression in the leaf and emerging primordia in the cells along the path of developing veins
Gandotra N, Coughlan SJ, Nelson T - The Arabidopsis leaf provascular cell transcriptome is enriched in genes with roles in vein patterning
Visit the PubMed ArticleRD20 ... under salinity stress ... reduced in wrky8 mutants
Hu Y, Chen L, Wang H, Zhang L, Wang F, Yu D - Arabidopsis transcription factor WRKY8 functions antagonistically with its interacting partner VQ9 to modulate salinity stress tolerance
Visit the PubMed ArticleAs the VQ motif of VQ9 is important for the WRKY8–VQ9 interaction, the VQ9ΔVQ motif construct was also fused to GFP and expressed in leaves of tobacco. Interestingly, the VQ9ΔVQ motif construct displayed fluorescence in both the nucleus and the cytoplasm, sharing the same distribution as the smaller GFP
Hu Y, Chen L, Wang H, Zhang L, Wang F, Yu D - Arabidopsis transcription factor WRKY8 functions antagonistically with its interacting partner VQ9 to modulate salinity stress tolerance
Visit the PubMed ArticleVQ9 was strongly induced by NaCl treatment
Hu Y, Chen L, Wang H, Zhang L, Wang F, Yu D - Arabidopsis transcription factor WRKY8 functions antagonistically with its interacting partner VQ9 to modulate salinity stress tolerance
Visit the PubMed ArticleRD29B ... under salinity stress ... enhanced in vq9 mutants
Hu Y, Chen L, Wang H, Zhang L, Wang F, Yu D - Arabidopsis transcription factor WRKY8 functions antagonistically with its interacting partner VQ9 to modulate salinity stress tolerance
Visit the PubMed ArticleSOS1 ... expression levels were reduced in wrky8 mutants ... compared with those in wild-type plants, under salt treatment
Hu Y, Chen L, Wang H, Zhang L, Wang F, Yu D - Arabidopsis transcription factor WRKY8 functions antagonistically with its interacting partner VQ9 to modulate salinity stress tolerance
Visit the PubMed ArticleSOS2 ... expression levels were reduced in wrky8 mutants ... compared with those in wild-type plants, under salt treatment
Hu Y, Chen L, Wang H, Zhang L, Wang F, Yu D - Arabidopsis transcription factor WRKY8 functions antagonistically with its interacting partner VQ9 to modulate salinity stress tolerance
Visit the PubMed ArticleVQ9 ... was not induced by ... cold
Hu Y, Chen L, Wang H, Zhang L, Wang F, Yu D - Arabidopsis transcription factor WRKY8 functions antagonistically with its interacting partner VQ9 to modulate salinity stress tolerance
Visit the PubMed ArticleRD20 ... not affected in ... vq9
Hu Y, Chen L, Wang H, Zhang L, Wang F, Yu D - Arabidopsis transcription factor WRKY8 functions antagonistically with its interacting partner VQ9 to modulate salinity stress tolerance
Visit the PubMed ArticleRD29A ... under salinity stress ... reduced in wrky8 mutants
Hu Y, Chen L, Wang H, Zhang L, Wang F, Yu D - Arabidopsis transcription factor WRKY8 functions antagonistically with its interacting partner VQ9 to modulate salinity stress tolerance
Visit the PubMed ArticleWRKY8 expression was strongly upregulated by high-salinity treatment
Hu Y, Chen L, Wang H, Zhang L, Wang F, Yu D - Arabidopsis transcription factor WRKY8 functions antagonistically with its interacting partner VQ9 to modulate salinity stress tolerance
Visit the PubMed ArticleRD20 ... under salinity stress ... enhanced in vq9 mutants
Hu Y, Chen L, Wang H, Zhang L, Wang F, Yu D - Arabidopsis transcription factor WRKY8 functions antagonistically with its interacting partner VQ9 to modulate salinity stress tolerance
Visit the PubMed ArticleRD29A ... not affected in ... vq9
Hu Y, Chen L, Wang H, Zhang L, Wang F, Yu D - Arabidopsis transcription factor WRKY8 functions antagonistically with its interacting partner VQ9 to modulate salinity stress tolerance
Visit the PubMed ArticleSOS3 ... expression levels were ... increased in vq9 mutants ... compared with those in wild-type plants, under salt treatment
Hu Y, Chen L, Wang H, Zhang L, Wang F, Yu D - Arabidopsis transcription factor WRKY8 functions antagonistically with its interacting partner VQ9 to modulate salinity stress tolerance
Visit the PubMed ArticleSOS1 ... expression levels were ... increased in vq9 mutants, compared with those in wild-type plants, under salt treatment
Hu Y, Chen L, Wang H, Zhang L, Wang F, Yu D - Arabidopsis transcription factor WRKY8 functions antagonistically with its interacting partner VQ9 to modulate salinity stress tolerance
Visit the PubMed ArticleADH1 ... under salinity stress ... enhanced in vq9 mutants
Hu Y, Chen L, Wang H, Zhang L, Wang F, Yu D - Arabidopsis transcription factor WRKY8 functions antagonistically with its interacting partner VQ9 to modulate salinity stress tolerance
Visit the PubMed Articlewe detected high levels of WRKY8 transcripts in roots and low levels in rosette leaves and siliques. WRKY8 transcripts were barely detected in cauline leaves, stems and flowers
Hu Y, Chen L, Wang H, Zhang L, Wang F, Yu D - Arabidopsis transcription factor WRKY8 functions antagonistically with its interacting partner VQ9 to modulate salinity stress tolerance
Visit the PubMed ArticleFirst, we determined its subcellular localization by fusing the full-length VQ9 to green fluorescent protein (GFP) and transiently expressing the construct in leaves of tobacco. As shown in Figures 5(a) and S1b, the transiently expressed VQ9-GFP fusion protein was exclusively localized in the nucleus, as revealed by DAPI staining
Hu Y, Chen L, Wang H, Zhang L, Wang F, Yu D - Arabidopsis transcription factor WRKY8 functions antagonistically with its interacting partner VQ9 to modulate salinity stress tolerance
Visit the PubMed ArticleRD29B ... not affected in ... vq9
Hu Y, Chen L, Wang H, Zhang L, Wang F, Yu D - Arabidopsis transcription factor WRKY8 functions antagonistically with its interacting partner VQ9 to modulate salinity stress tolerance
Visit the PubMed ArticleRD29B ... not affected in wrky8
Hu Y, Chen L, Wang H, Zhang L, Wang F, Yu D - Arabidopsis transcription factor WRKY8 functions antagonistically with its interacting partner VQ9 to modulate salinity stress tolerance
Visit the PubMed ArticleADH1 ... not affected in wrky8
Hu Y, Chen L, Wang H, Zhang L, Wang F, Yu D - Arabidopsis transcription factor WRKY8 functions antagonistically with its interacting partner VQ9 to modulate salinity stress tolerance
Visit the PubMed ArticleADH1 ... not affected in ... vq9
Hu Y, Chen L, Wang H, Zhang L, Wang F, Yu D - Arabidopsis transcription factor WRKY8 functions antagonistically with its interacting partner VQ9 to modulate salinity stress tolerance
Visit the PubMed ArticleVQ9 was ... moderately induced by dehydration
Hu Y, Chen L, Wang H, Zhang L, Wang F, Yu D - Arabidopsis transcription factor WRKY8 functions antagonistically with its interacting partner VQ9 to modulate salinity stress tolerance
Visit the PubMed ArticleRD29B ... under salinity stress ... reduced in wrky8 mutants
Hu Y, Chen L, Wang H, Zhang L, Wang F, Yu D - Arabidopsis transcription factor WRKY8 functions antagonistically with its interacting partner VQ9 to modulate salinity stress tolerance
Visit the PubMed ArticleRD29A ... under salinity stress ... enhanced in vq9 mutants
Hu Y, Chen L, Wang H, Zhang L, Wang F, Yu D - Arabidopsis transcription factor WRKY8 functions antagonistically with its interacting partner VQ9 to modulate salinity stress tolerance
Visit the PubMed ArticleSOS3 ... expression levels were reduced in wrky8 mutants ... compared with those in wild-type plants, under salt treatment
Hu Y, Chen L, Wang H, Zhang L, Wang F, Yu D - Arabidopsis transcription factor WRKY8 functions antagonistically with its interacting partner VQ9 to modulate salinity stress tolerance
Visit the PubMed ArticleRD20 ... not affected in wrky8
Hu Y, Chen L, Wang H, Zhang L, Wang F, Yu D - Arabidopsis transcription factor WRKY8 functions antagonistically with its interacting partner VQ9 to modulate salinity stress tolerance
Visit the PubMed ArticleSOS2 ... expression levels were ... increased in vq9 mutants ... compared with those in wild-type plants, under salt treatment
Hu Y, Chen L, Wang H, Zhang L, Wang F, Yu D - Arabidopsis transcription factor WRKY8 functions antagonistically with its interacting partner VQ9 to modulate salinity stress tolerance
Visit the PubMed ArticleVQ9 ... was not induced by heat
Hu Y, Chen L, Wang H, Zhang L, Wang F, Yu D - Arabidopsis transcription factor WRKY8 functions antagonistically with its interacting partner VQ9 to modulate salinity stress tolerance
Visit the PubMed ArticleADH1 ... under salinity stress ... reduced in wrky8 mutants
Hu Y, Chen L, Wang H, Zhang L, Wang F, Yu D - Arabidopsis transcription factor WRKY8 functions antagonistically with its interacting partner VQ9 to modulate salinity stress tolerance
Visit the PubMed ArticleRD29A ... not affected in wrky8
Hu Y, Chen L, Wang H, Zhang L, Wang F, Yu D - Arabidopsis transcription factor WRKY8 functions antagonistically with its interacting partner VQ9 to modulate salinity stress tolerance
Visit the PubMed Articlebsk6 ... retained significantly higher DWF4 expression compared to wild-type plants ... upon BL treatment
Sreeramulu S, Mostizky Y, Sunitha S, Shani E, Nahum H, Salomon D, Hayun LB, Gruetter C, Rauh D, Ori N, Sessa G - BSKs are partially redundant positive regulators of brassinosteroid signaling in Arabidopsis
Visit the PubMed Articlebsk3,4,6,8 ... caused significant de-repression of DWF4 expression upon BL treatment
Sreeramulu S, Mostizky Y, Sunitha S, Shani E, Nahum H, Salomon D, Hayun LB, Gruetter C, Rauh D, Ori N, Sessa G - BSKs are partially redundant positive regulators of brassinosteroid signaling in Arabidopsis
Visit the PubMed Articlebsk7 ... retained significantly higher DWF4 expression compared to wild-type plants ... upon BL treatment
Sreeramulu S, Mostizky Y, Sunitha S, Shani E, Nahum H, Salomon D, Hayun LB, Gruetter C, Rauh D, Ori N, Sessa G - BSKs are partially redundant positive regulators of brassinosteroid signaling in Arabidopsis
Visit the PubMed Articleto BL treatment ... the pentuple mutant bsk1,3,4,6,7 displayed a DWF4 expression pattern similar to that of the wild-type
Sreeramulu S, Mostizky Y, Sunitha S, Shani E, Nahum H, Salomon D, Hayun LB, Gruetter C, Rauh D, Ori N, Sessa G - BSKs are partially redundant positive regulators of brassinosteroid signaling in Arabidopsis
Visit the PubMed ArticleSAUR-AC1 expression was only marginally induced by BL treatment in the ... bsk7,8 double mutants
Sreeramulu S, Mostizky Y, Sunitha S, Shani E, Nahum H, Salomon D, Hayun LB, Gruetter C, Rauh D, Ori N, Sessa G - BSKs are partially redundant positive regulators of brassinosteroid signaling in Arabidopsis
Visit the PubMed ArticleUpon treatment of bsk mutants with BL, the ... bsk4 ... single mutants displayed a significantly reduced induction of SAUR-AC1 expression compared to the wild-type
Sreeramulu S, Mostizky Y, Sunitha S, Shani E, Nahum H, Salomon D, Hayun LB, Gruetter C, Rauh D, Ori N, Sessa G - BSKs are partially redundant positive regulators of brassinosteroid signaling in Arabidopsis
Visit the PubMed Articlebsk2 ... retained significantly higher DWF4 expression compared to wild-type plants ... upon BL treatment
Sreeramulu S, Mostizky Y, Sunitha S, Shani E, Nahum H, Salomon D, Hayun LB, Gruetter C, Rauh D, Ori N, Sessa G - BSKs are partially redundant positive regulators of brassinosteroid signaling in Arabidopsis
Visit the PubMed Articlebsk5 ... retained significantly higher DWF4 expression compared to wild-type plants ... upon BL treatment
Sreeramulu S, Mostizky Y, Sunitha S, Shani E, Nahum H, Salomon D, Hayun LB, Gruetter C, Rauh D, Ori N, Sessa G - BSKs are partially redundant positive regulators of brassinosteroid signaling in Arabidopsis
Visit the PubMed ArticleSAUR-AC1 expression was only marginally induced by BL treatment in the bsk3,4 ... double mutants
Sreeramulu S, Mostizky Y, Sunitha S, Shani E, Nahum H, Salomon D, Hayun LB, Gruetter C, Rauh D, Ori N, Sessa G - BSKs are partially redundant positive regulators of brassinosteroid signaling in Arabidopsis
Visit the PubMed ArticleUpon treatment of bsk mutants with BL, the bsk1 ... single mutants displayed a significantly reduced induction of SAUR-AC1 expression compared to the wild-type. The SAUR-AC1 expression level was significantly higher in the bsk1 mutant than in the wild-type in the absence or presence of BL
Sreeramulu S, Mostizky Y, Sunitha S, Shani E, Nahum H, Salomon D, Hayun LB, Gruetter C, Rauh D, Ori N, Sessa G - BSKs are partially redundant positive regulators of brassinosteroid signaling in Arabidopsis
Visit the PubMed ArticleSAUR-AC1 expression ... by BL treatment ... was not induced at all in the ... bsk3,4,6,7,8 pentuple mutant
Sreeramulu S, Mostizky Y, Sunitha S, Shani E, Nahum H, Salomon D, Hayun LB, Gruetter C, Rauh D, Ori N, Sessa G - BSKs are partially redundant positive regulators of brassinosteroid signaling in Arabidopsis
Visit the PubMed ArticleAs expected, BL treatment in wild-type Col-0 seedlings reduced the expression of DWF4 to almost 12% relative to untreated plants
Sreeramulu S, Mostizky Y, Sunitha S, Shani E, Nahum H, Salomon D, Hayun LB, Gruetter C, Rauh D, Ori N, Sessa G - BSKs are partially redundant positive regulators of brassinosteroid signaling in Arabidopsis
Visit the PubMed Articlebsk3,4,6,7,8 ... caused significant de-repression of DWF4 expression upon BL treatment
Sreeramulu S, Mostizky Y, Sunitha S, Shani E, Nahum H, Salomon D, Hayun LB, Gruetter C, Rauh D, Ori N, Sessa G - BSKs are partially redundant positive regulators of brassinosteroid signaling in Arabidopsis
Visit the PubMed ArticleUpon treatment of bsk mutants with BL, the ... bsk5 single mutants displayed a significantly reduced induction of SAUR-AC1 expression compared to the wild-type
Sreeramulu S, Mostizky Y, Sunitha S, Shani E, Nahum H, Salomon D, Hayun LB, Gruetter C, Rauh D, Ori N, Sessa G - BSKs are partially redundant positive regulators of brassinosteroid signaling in Arabidopsis
Visit the PubMed Articlebsk3,4,7,8 ... caused significant de-repression of DWF4 expression upon BL treatment
Sreeramulu S, Mostizky Y, Sunitha S, Shani E, Nahum H, Salomon D, Hayun LB, Gruetter C, Rauh D, Ori N, Sessa G - BSKs are partially redundant positive regulators of brassinosteroid signaling in Arabidopsis
Visit the PubMed ArticleDWF4 regulation by BL was similar to wild-type in the bsk7,8 double mutant
Sreeramulu S, Mostizky Y, Sunitha S, Shani E, Nahum H, Salomon D, Hayun LB, Gruetter C, Rauh D, Ori N, Sessa G - BSKs are partially redundant positive regulators of brassinosteroid signaling in Arabidopsis
Visit the PubMed Articlebsk3,4,7 ... caused significant de-repression of DWF4 expression upon BL treatment
Sreeramulu S, Mostizky Y, Sunitha S, Shani E, Nahum H, Salomon D, Hayun LB, Gruetter C, Rauh D, Ori N, Sessa G - BSKs are partially redundant positive regulators of brassinosteroid signaling in Arabidopsis
Visit the PubMed Articlebsk3 ... retained significantly higher DWF4 expression compared to wild-type plants ... upon BL treatment
Sreeramulu S, Mostizky Y, Sunitha S, Shani E, Nahum H, Salomon D, Hayun LB, Gruetter C, Rauh D, Ori N, Sessa G - BSKs are partially redundant positive regulators of brassinosteroid signaling in Arabidopsis
Visit the PubMed ArticleSAUR-AC1 expression was induced approximately threefold in wild-type Col-0 Arabidopsis seedlings in response to BL
Sreeramulu S, Mostizky Y, Sunitha S, Shani E, Nahum H, Salomon D, Hayun LB, Gruetter C, Rauh D, Ori N, Sessa G - BSKs are partially redundant positive regulators of brassinosteroid signaling in Arabidopsis
Visit the PubMed ArticleSAUR-AC1 expression ... by BL treatment ... was not induced at all in the bsk3,4,7,8 quadruple mutant
Sreeramulu S, Mostizky Y, Sunitha S, Shani E, Nahum H, Salomon D, Hayun LB, Gruetter C, Rauh D, Ori N, Sessa G - BSKs are partially redundant positive regulators of brassinosteroid signaling in Arabidopsis
Visit the PubMed Articlewhen a mutation in BSK8 was introduced into the bsk3,4 background, which showed impaired DWF4 regulation by BL, repression of DWF4 in the resulting bsk3,4,8 triple mutant was similar to wild-type
Sreeramulu S, Mostizky Y, Sunitha S, Shani E, Nahum H, Salomon D, Hayun LB, Gruetter C, Rauh D, Ori N, Sessa G - BSKs are partially redundant positive regulators of brassinosteroid signaling in Arabidopsis
Visit the PubMed Articlebsk3,4 ... caused significant de-repression of DWF4 expression upon BL treatment
Sreeramulu S, Mostizky Y, Sunitha S, Shani E, Nahum H, Salomon D, Hayun LB, Gruetter C, Rauh D, Ori N, Sessa G - BSKs are partially redundant positive regulators of brassinosteroid signaling in Arabidopsis
Visit the PubMed Articlebsk3,4,6,7 ... caused significant de-repression of DWF4 expression upon BL treatment
Sreeramulu S, Mostizky Y, Sunitha S, Shani E, Nahum H, Salomon D, Hayun LB, Gruetter C, Rauh D, Ori N, Sessa G - BSKs are partially redundant positive regulators of brassinosteroid signaling in Arabidopsis
Visit the PubMed Articleto BL treatment ... the bsk3,4,6 triple mutant displayed a DWF4 expression pattern similar to that of the wild-type
Sreeramulu S, Mostizky Y, Sunitha S, Shani E, Nahum H, Salomon D, Hayun LB, Gruetter C, Rauh D, Ori N, Sessa G - BSKs are partially redundant positive regulators of brassinosteroid signaling in Arabidopsis
Visit the PubMed ArticleTo exclude the possibility that high expression of HDG2 in the meristemoid-enriched population may simply be reflecting the protodermal character of the actively dividing scrm-D mute epidermis, we further surveyed the expression of AtML1 ... in stomatal cell-state mutants. Unlike HDG2, both AtML1 ... showed moderately high expression in all genetic backgrounds (Fig. 1D). The expression levels of AtML1 ... were only 1.5-fold ... higher in scrm-D mute compared with wild type, which are significantly less than the more than 18-fold increase in HDG2
Peterson KM, Shyu C, Burr CA, Horst RJ, Kanaoka MM, Omae M, Sato Y, Torii KU - Arabidopsis homeodomain-leucine zipper IV proteins promote stomatal development and ectopically induce stomata beyond the epidermis
Visit the PubMed Articlewe did not detect clear ectopic expression of a trichome marker in GL2pro::GUS seedlings nor trichome differentiation in internal tissues in HDG2-OX
Peterson KM, Shyu C, Burr CA, Horst RJ, Kanaoka MM, Omae M, Sato Y, Torii KU - Arabidopsis homeodomain-leucine zipper IV proteins promote stomatal development and ectopically induce stomata beyond the epidermis
Visit the PubMed ArticleWe next examined the cell-type expression patterns of HDG2 in developing cotyledon and leaf epidermis. HDG2 transcriptional reporter (HDG2pro::nls-3xGFP) showed strong GFP signals in meristemoids and SLGCs
Peterson KM, Shyu C, Burr CA, Horst RJ, Kanaoka MM, Omae M, Sato Y, Torii KU - Arabidopsis homeodomain-leucine zipper IV proteins promote stomatal development and ectopically induce stomata beyond the epidermis
Visit the PubMed ArticleWe next examined the expression of the L1-layer-specific gene FIDDLEHEAD (FDH) (Pruitt et al., 2000) using in situ hybridization (Fig. 3C-F). As expected, strong FDH signals are detected in the epidermis of both wild-type and HDG2-OX seedlings (Fig. 3C,E); however, it was inconclusive whether FDH is ectopically expressed in the internal tissues of HDG2-OX seedlings
Peterson KM, Shyu C, Burr CA, Horst RJ, Kanaoka MM, Omae M, Sato Y, Torii KU - Arabidopsis homeodomain-leucine zipper IV proteins promote stomatal development and ectopically induce stomata beyond the epidermis
Visit the PubMed ArticleIn situ hybridization analysis of total HDG2 transcripts showed strong expression in small epidermal cells (Fig. 2A), confirming the meristemoid-enriched expression seen in scrm-D mute seedlings, as well as in HDG2 GFP reporters
Peterson KM, Shyu C, Burr CA, Horst RJ, Kanaoka MM, Omae M, Sato Y, Torii KU - Arabidopsis homeodomain-leucine zipper IV proteins promote stomatal development and ectopically induce stomata beyond the epidermis
Visit the PubMed ArticleTo unravel whether HDG2 can activate earlier steps of stomatal differentiation, we next examined whether HDG2-OX triggers ectopic expression of earlier stomatal cell-lineage markers. As shown in Fig. 2J,K, a subpopulation of HDG2-OX mesophyll cells expresses TMMpro::GUS-GFP, which is normally expressed in early stomatal-lineage cells, such as meristemoids and SLGCs (Nadeau and Sack, 2002). The TMM-negative internal cells are round and have numerous chloroplasts (Fig. 2J,K), indicating that they probably retain mesophyll cell identity
Peterson KM, Shyu C, Burr CA, Horst RJ, Kanaoka MM, Omae M, Sato Y, Torii KU - Arabidopsis homeodomain-leucine zipper IV proteins promote stomatal development and ectopically induce stomata beyond the epidermis
Visit the PubMed ArticleWe therefore used an endoreduplicating epidermal cell marker (At5g17710pro::nuc-3xVENUS) (Roeder et al., 2012) to address this issue (Fig. 3G-I). The marker was originally identified for sepal epidermal ‘giant’ cells, but it also exhibits expression in large pavement cells in cotyledons and rosette leaves (Fig. 3G; data not shown). The At5g17710pro::nuc-3xVENUS signal was detected in occasional subepidermal nuclei in HDG2-OX seedlings
Peterson KM, Shyu C, Burr CA, Horst RJ, Kanaoka MM, Omae M, Sato Y, Torii KU - Arabidopsis homeodomain-leucine zipper IV proteins promote stomatal development and ectopically induce stomata beyond the epidermis
Visit the PubMed Articlethe HDG2 translational reporter (HDG2pro::HDG2-GFP) accumulated in the nuclei of meristemoids and SLGCs
Peterson KM, Shyu C, Burr CA, Horst RJ, Kanaoka MM, Omae M, Sato Y, Torii KU - Arabidopsis homeodomain-leucine zipper IV proteins promote stomatal development and ectopically induce stomata beyond the epidermis
Visit the PubMed Articlewhether HDG2 overexpression confers ectopic epidermal fate. Indeed, the L1-layer marker AtML1pro::GUS was occasionally detected in the internal tissues
Peterson KM, Shyu C, Burr CA, Horst RJ, Kanaoka MM, Omae M, Sato Y, Torii KU - Arabidopsis homeodomain-leucine zipper IV proteins promote stomatal development and ectopically induce stomata beyond the epidermis
Visit the PubMed ArticleTo exclude the possibility that high expression of HDG2 in the meristemoid-enriched population may simply be reflecting the protodermal character of the actively dividing scrm-D mute epidermis, we further surveyed the expression of ... PDF2 in stomatal cell-state mutants. Unlike HDG2 ... PDF2 showed moderately high expression in all genetic backgrounds ... The expression levels of ... PDF2 were only ... 1.4-fold higher in scrm-D mute compared with wild type, which are significantly less than the more than 18-fold increase in HDG2
Peterson KM, Shyu C, Burr CA, Horst RJ, Kanaoka MM, Omae M, Sato Y, Torii KU - Arabidopsis homeodomain-leucine zipper IV proteins promote stomatal development and ectopically induce stomata beyond the epidermis
Visit the PubMed ArticleWe have previously performed a comparative transcriptomic analysis of seedlings specifically enriched in particular stomatal cell state: spch, in which epidermis is devoid of any stomatal-lineage cells (i.e. pavement cells only); scrm-D mute, which produces an epidermis solely composed of meristemoids and sister cells (known as stomatal lineage ground cells, SLGCs); and scrm-D, which produces a stomata-only epidermis ... we identified HDG2 ... as a meristemoid-enriched gene. HDG2 shows strikingly high expression in the meristemoid-enriched population (2069.7±200.8, mean±s.e.m.) with over 18-fold, 20-fold and 10-fold enrichment over wild type (114.6±4.76), spch (102.2±18.3) and scrm-D (200.5±9.02), respectively ... Quantitative RT-PCR analysis confirmed specific and high HDG2 expression in scrm-D mute, with over 31-fold, 62-fold and 18-fold expression compared with wild type, spch and scrm-D, respectively
Peterson KM, Shyu C, Burr CA, Horst RJ, Kanaoka MM, Omae M, Sato Y, Torii KU - Arabidopsis homeodomain-leucine zipper IV proteins promote stomatal development and ectopically induce stomata beyond the epidermis
Visit the PubMed ArticleTo unravel whether HDG2 can activate earlier steps of stomatal differentiation, we next examined whether HDG2-OX triggers ectopic expression of earlier stomatal cell-lineage markers ... As shown in Fig. 2J,K, a subpopulation of HDG2-OX mesophyll cells ... We further detected the expression of SPCHpro::GUS, the earliest marker of stomatal cell lineages, in the mesophyll tissue
Peterson KM, Shyu C, Burr CA, Horst RJ, Kanaoka MM, Omae M, Sato Y, Torii KU - Arabidopsis homeodomain-leucine zipper IV proteins promote stomatal development and ectopically induce stomata beyond the epidermis
Visit the PubMed ArticleICK2 ... repress cell division ... down-regulated by approximately one-third ... in fk–J3158
Qian P, Han B, Forestier E, Hu Z, Gao N, Lu W, Schaller H, Li J, Hou S - Sterols are required for cell-fate commitment and maintenance of the stomatal lineage in Arabidopsis
Visit the PubMed ArticleFEN-treated Col-0 were labeled by ... pCYCB1;1::CYCB1;1-GUS, which ... expressed in early stomatal lineage cells, including meristemoid mother cells, meristemoids and guard mother cells ... We found that ... GUS ... detected in some clustered small cells, but not in those that likely withdrew from stomatal lineage cell division and finally converted to small pavement cell clusters
Qian P, Han B, Forestier E, Hu Z, Gao N, Lu W, Schaller H, Li J, Hou S - Sterols are required for cell-fate commitment and maintenance of the stomatal lineage in Arabidopsis
Visit the PubMed Articleexpression of ... CYCD3 in fk–J3158 was significantly increased by approximately ... three times
Qian P, Han B, Forestier E, Hu Z, Gao N, Lu W, Schaller H, Li J, Hou S - Sterols are required for cell-fate commitment and maintenance of the stomatal lineage in Arabidopsis
Visit the PubMed ArticleIn fk–J3158 and FEN-treated Col-0, pMUTE::GFP signals were observed in some small-cell clusters (Figure 9e,f), suggesting that the asymmetric expression pattern of MUTE in the stomatal lineage is lost in the fk mutants, and that the clustered small cells expressing MUTE will most likely undergo terminal differentiation and form clustered stomata
Qian P, Han B, Forestier E, Hu Z, Gao N, Lu W, Schaller H, Li J, Hou S - Sterols are required for cell-fate commitment and maintenance of the stomatal lineage in Arabidopsis
Visit the PubMed ArticleIn fk–J3158 ... CYCA2;1 ... down-regulated
Qian P, Han B, Forestier E, Hu Z, Gao N, Lu W, Schaller H, Li J, Hou S - Sterols are required for cell-fate commitment and maintenance of the stomatal lineage in Arabidopsis
Visit the PubMed ArticleIn fk–J3158 ... CYCA2;2 ... down-regulated
Qian P, Han B, Forestier E, Hu Z, Gao N, Lu W, Schaller H, Li J, Hou S - Sterols are required for cell-fate commitment and maintenance of the stomatal lineage in Arabidopsis
Visit the PubMed Articleexpression of the asymmetric cell division marker pBASL::GFP-BASL was strongly polarized in the clustered small cells of FEN-treated Col-0 (Figure 9m–o and Figure S8g–i). Time-lapse imaging revealed that, during the mitotic division of small-cell clusters, pBASL::GFP-BASL exhibited polarized localization in every cell membrane
Qian P, Han B, Forestier E, Hu Z, Gao N, Lu W, Schaller H, Li J, Hou S - Sterols are required for cell-fate commitment and maintenance of the stomatal lineage in Arabidopsis
Visit the PubMed ArticleICK1 ... repress cell division ... down-regulated by approximately one-third ... in fk–J3158
Qian P, Han B, Forestier E, Hu Z, Gao N, Lu W, Schaller H, Li J, Hou S - Sterols are required for cell-fate commitment and maintenance of the stomatal lineage in Arabidopsis
Visit the PubMed ArticleFEN-treated Col-0 ... CYCA2;1 ... down-regulated
Qian P, Han B, Forestier E, Hu Z, Gao N, Lu W, Schaller H, Li J, Hou S - Sterols are required for cell-fate commitment and maintenance of the stomatal lineage in Arabidopsis
Visit the PubMed Articlemeasurement of the division asymmetry using TUB6-labeled preprophase bands of microtubules, cytokinesis and phragmoplasts, in Col-0 and fk–J3158 leaves also showed similar results (n = 200 cells)
Qian P, Han B, Forestier E, Hu Z, Gao N, Lu W, Schaller H, Li J, Hou S - Sterols are required for cell-fate commitment and maintenance of the stomatal lineage in Arabidopsis
Visit the PubMed ArticleWe quantified two daughter cell sizes from asymmetric division, and observed expression of the asymmetric cell division (ACD) marker (Dong et al., 2009). In Col-0 leaves, 70% of meristemoid mother cell and meristemoid divisions resulted in the smaller cells being <40% of the combined area of the two daughter cells. Similarly, in fk–J3158, 72% of meristemoid mother cell and meristemoid divisions that produced daughter cells undergoing incorrect cell division or cell fate (detected by time-lapse agarose impressions) exhibited the same size asymmetry as in Col-0 (n = 180 cell pairs) (Figure 8 and Figure S7).
Qian P, Han B, Forestier E, Hu Z, Gao N, Lu W, Schaller H, Li J, Hou S - Sterols are required for cell-fate commitment and maintenance of the stomatal lineage in Arabidopsis
Visit the PubMed Articleexpression of CYCB1 ... in fk–J3158 was significantly increased by approximately one-half ... times ... compared to wild-type
Qian P, Han B, Forestier E, Hu Z, Gao N, Lu W, Schaller H, Li J, Hou S - Sterols are required for cell-fate commitment and maintenance of the stomatal lineage in Arabidopsis
Visit the PubMed ArticleE2FC ... repress cell division ... down-regulated by approximately one-third ... in fk–J3158
Qian P, Han B, Forestier E, Hu Z, Gao N, Lu W, Schaller H, Li J, Hou S - Sterols are required for cell-fate commitment and maintenance of the stomatal lineage in Arabidopsis
Visit the PubMed Articlefk–J3158 ... pCYCB1;1::CYCB1;1-GUS, which ... expressed in early stomatal lineage cells, including meristemoid mother cells, meristemoids and guard mother cells ... We found that ... GUS ... detected in some clustered small cells, but not in those that likely withdrew from stomatal lineage cell division and finally converted to small pavement cell clusters
Qian P, Han B, Forestier E, Hu Z, Gao N, Lu W, Schaller H, Li J, Hou S - Sterols are required for cell-fate commitment and maintenance of the stomatal lineage in Arabidopsis
Visit the PubMed ArticleFEN-treated Col-0 were labeled by pTMM::TMM-GFP ... which ... expressed in early stomatal lineage cells, including meristemoid mother cells, meristemoids and guard mother cells ... We found that GFP ... detected in some clustered small cells, but not in those that likely withdrew from stomatal lineage cell division and finally converted to small pavement cell clusters
Qian P, Han B, Forestier E, Hu Z, Gao N, Lu W, Schaller H, Li J, Hou S - Sterols are required for cell-fate commitment and maintenance of the stomatal lineage in Arabidopsis
Visit the PubMed ArticleSKP1 ... repress cell division ... down-regulated by approximately one-third ... in fk–J3158
Qian P, Han B, Forestier E, Hu Z, Gao N, Lu W, Schaller H, Li J, Hou S - Sterols are required for cell-fate commitment and maintenance of the stomatal lineage in Arabidopsis
Visit the PubMed ArticleFEN-treated Col-0 ... CYCA2;2 ... down-regulated
Qian P, Han B, Forestier E, Hu Z, Gao N, Lu W, Schaller H, Li J, Hou S - Sterols are required for cell-fate commitment and maintenance of the stomatal lineage in Arabidopsis
Visit the PubMed Articlefk–J3158 ... labeled by pTMM::TMM-GFP ... which ... expressed in early stomatal lineage cells, including meristemoid mother cells, meristemoids and guard mother cells ... We found that GFP ... detected in some clustered small cells, but not in those that likely withdrew from stomatal lineage cell division and finally converted to small pavement cell clusters
Qian P, Han B, Forestier E, Hu Z, Gao N, Lu W, Schaller H, Li J, Hou S - Sterols are required for cell-fate commitment and maintenance of the stomatal lineage in Arabidopsis
Visit the PubMed ArticleWe confirmed elevated levels of ... KNAT2 expression in STMoe lines
Scofield S, Dewitte W, Nieuwland J, Murray JA - The Arabidopsis homeobox gene SHOOT MERISTEMLESS has cellular and meristem-organisational roles with differential requirements for cytokinin and CYCD3 activity
Visit the PubMed ArticleWe detected elevated levels of ... WOL in ... KNAT1oe ... leaves
Scofield S, Dewitte W, Nieuwland J, Murray JA - The Arabidopsis homeobox gene SHOOT MERISTEMLESS has cellular and meristem-organisational roles with differential requirements for cytokinin and CYCD3 activity
Visit the PubMed Articlewe examined expression of CYCD3;1 ... the lobes at the leaf margin coincided with foci of GUS activity ... in KNAT1oe ... leaves
Scofield S, Dewitte W, Nieuwland J, Murray JA - The Arabidopsis homeobox gene SHOOT MERISTEMLESS has cellular and meristem-organisational roles with differential requirements for cytokinin and CYCD3 activity
Visit the PubMed ArticleWe confirmed WUS expression in ectopic meristems (in STMoe) using a pWUS:GUS reporter
Scofield S, Dewitte W, Nieuwland J, Murray JA - The Arabidopsis homeobox gene SHOOT MERISTEMLESS has cellular and meristem-organisational roles with differential requirements for cytokinin and CYCD3 activity
Visit the PubMed ArticleCKX1 overexpression reduces levels of CK and expression of ARR6 ... compared with WT
Scofield S, Dewitte W, Nieuwland J, Murray JA - The Arabidopsis homeobox gene SHOOT MERISTEMLESS has cellular and meristem-organisational roles with differential requirements for cytokinin and CYCD3 activity
Visit the PubMed ArticleWe detected elevated levels of ... WOL in ... KNAT2oe leaves
Scofield S, Dewitte W, Nieuwland J, Murray JA - The Arabidopsis homeobox gene SHOOT MERISTEMLESS has cellular and meristem-organisational roles with differential requirements for cytokinin and CYCD3 activity
Visit the PubMed ArticleWe detected elevated levels of ... WOL in STMoe ... leaves
Scofield S, Dewitte W, Nieuwland J, Murray JA - The Arabidopsis homeobox gene SHOOT MERISTEMLESS has cellular and meristem-organisational roles with differential requirements for cytokinin and CYCD3 activity
Visit the PubMed ArticleWe detected elevated levels of ARR6 ... in STMoe ... leaves
Scofield S, Dewitte W, Nieuwland J, Murray JA - The Arabidopsis homeobox gene SHOOT MERISTEMLESS has cellular and meristem-organisational roles with differential requirements for cytokinin and CYCD3 activity
Visit the PubMed Articleconversely found no increase in STM mRNA in ... KNAT2oe lines
Scofield S, Dewitte W, Nieuwland J, Murray JA - The Arabidopsis homeobox gene SHOOT MERISTEMLESS has cellular and meristem-organisational roles with differential requirements for cytokinin and CYCD3 activity
Visit the PubMed ArticleCYCD3;2 ... transcript levels were reduced in the CKX1oe plants
Scofield S, Dewitte W, Nieuwland J, Murray JA - The Arabidopsis homeobox gene SHOOT MERISTEMLESS has cellular and meristem-organisational roles with differential requirements for cytokinin and CYCD3 activity
Visit the PubMed ArticleWe detected elevated levels of ARR6 ... in ... KNAT2oe leaves
Scofield S, Dewitte W, Nieuwland J, Murray JA - The Arabidopsis homeobox gene SHOOT MERISTEMLESS has cellular and meristem-organisational roles with differential requirements for cytokinin and CYCD3 activity
Visit the PubMed ArticleCYCD3;3 ... transcript levels were reduced in the CKX1oe plants
Scofield S, Dewitte W, Nieuwland J, Murray JA - The Arabidopsis homeobox gene SHOOT MERISTEMLESS has cellular and meristem-organisational roles with differential requirements for cytokinin and CYCD3 activity
Visit the PubMed ArticleCK promotes expression of ... CYCD3;2
Scofield S, Dewitte W, Nieuwland J, Murray JA - The Arabidopsis homeobox gene SHOOT MERISTEMLESS has cellular and meristem-organisational roles with differential requirements for cytokinin and CYCD3 activity
Visit the PubMed Articlewe examined expression of CYCD3;1 ... the lobes at the leaf margin coincided with foci of GUS activity ... in ... KNAT2oe leaves
Scofield S, Dewitte W, Nieuwland J, Murray JA - The Arabidopsis homeobox gene SHOOT MERISTEMLESS has cellular and meristem-organisational roles with differential requirements for cytokinin and CYCD3 activity
Visit the PubMed ArticleCK promotes expression of ... CYCD3;3
Scofield S, Dewitte W, Nieuwland J, Murray JA - The Arabidopsis homeobox gene SHOOT MERISTEMLESS has cellular and meristem-organisational roles with differential requirements for cytokinin and CYCD3 activity
Visit the PubMed ArticleIn WT plants, expression of an Arabidopsis pCYCD3;1:GUS reporter is initially detected throughout the leaf primordium and then becomes confined to hydathodes at the leaf margin as leaves develop towards maturity, coinciding with leaf serrations
Scofield S, Dewitte W, Nieuwland J, Murray JA - The Arabidopsis homeobox gene SHOOT MERISTEMLESS has cellular and meristem-organisational roles with differential requirements for cytokinin and CYCD3 activity
Visit the PubMed ArticleWe confirmed elevated levels of KNAT1/BP ... in STMoe lines
Scofield S, Dewitte W, Nieuwland J, Murray JA - The Arabidopsis homeobox gene SHOOT MERISTEMLESS has cellular and meristem-organisational roles with differential requirements for cytokinin and CYCD3 activity
Visit the PubMed ArticleWe observed prolonged and extensive ectopic cell division at the leaf margin and throughout developing lobes of STMoe leaves using the pCYCB1;1:CYCB1;1MDB-GUS reporter
Scofield S, Dewitte W, Nieuwland J, Murray JA - The Arabidopsis homeobox gene SHOOT MERISTEMLESS has cellular and meristem-organisational roles with differential requirements for cytokinin and CYCD3 activity
Visit the PubMed ArticleWe also found that KNAT1/BP ... down-regulated in inducible STM-RNAi lines following transient induction
Scofield S, Dewitte W, Nieuwland J, Murray JA - The Arabidopsis homeobox gene SHOOT MERISTEMLESS has cellular and meristem-organisational roles with differential requirements for cytokinin and CYCD3 activity
Visit the PubMed ArticleCKX1 overexpression reduces levels of CK and expression of ... WOL compared with WT
Scofield S, Dewitte W, Nieuwland J, Murray JA - The Arabidopsis homeobox gene SHOOT MERISTEMLESS has cellular and meristem-organisational roles with differential requirements for cytokinin and CYCD3 activity
Visit the PubMed ArticleCYCD3;1 ... transcript levels were reduced in the CKX1oe plants
Scofield S, Dewitte W, Nieuwland J, Murray JA - The Arabidopsis homeobox gene SHOOT MERISTEMLESS has cellular and meristem-organisational roles with differential requirements for cytokinin and CYCD3 activity
Visit the PubMed Articlein STMoe plants ... in developing ectopic meristems ... pCYCB1;1:CYCB1;1MDB-GUS activity was also detected
Scofield S, Dewitte W, Nieuwland J, Murray JA - The Arabidopsis homeobox gene SHOOT MERISTEMLESS has cellular and meristem-organisational roles with differential requirements for cytokinin and CYCD3 activity
Visit the PubMed ArticleCK promotes expression of ... CYCD3;1
Scofield S, Dewitte W, Nieuwland J, Murray JA - The Arabidopsis homeobox gene SHOOT MERISTEMLESS has cellular and meristem-organisational roles with differential requirements for cytokinin and CYCD3 activity
Visit the PubMed ArticleWe also detected increased expression of the cyclin-dependent kinase CDKB1;1, which is involved in inhibiting endoreduplication (Boudolf et al., 2004), in STMoe leaves
Scofield S, Dewitte W, Nieuwland J, Murray JA - The Arabidopsis homeobox gene SHOOT MERISTEMLESS has cellular and meristem-organisational roles with differential requirements for cytokinin and CYCD3 activity
Visit the PubMed Articlewe examined expression of CYCD3;1 in STMoe plants ... In the STMoe background, the lobes at the leaf margin coincided with foci of GUS activity
Scofield S, Dewitte W, Nieuwland J, Murray JA - The Arabidopsis homeobox gene SHOOT MERISTEMLESS has cellular and meristem-organisational roles with differential requirements for cytokinin and CYCD3 activity
Visit the PubMed Articlewe examined expression of CYCD3;1 in STMoe plants ... pCYCD3;1:GUS ... was expressed in developing ectopic meristems
Scofield S, Dewitte W, Nieuwland J, Murray JA - The Arabidopsis homeobox gene SHOOT MERISTEMLESS has cellular and meristem-organisational roles with differential requirements for cytokinin and CYCD3 activity
Visit the PubMed ArticleWe detected elevated levels of ARR6 ... in ... KNAT1oe ... leaves
Scofield S, Dewitte W, Nieuwland J, Murray JA - The Arabidopsis homeobox gene SHOOT MERISTEMLESS has cellular and meristem-organisational roles with differential requirements for cytokinin and CYCD3 activity
Visit the PubMed ArticleWe also found that ... KNAT2 ... down-regulated in inducible STM-RNAi lines following transient induction
Scofield S, Dewitte W, Nieuwland J, Murray JA - The Arabidopsis homeobox gene SHOOT MERISTEMLESS has cellular and meristem-organisational roles with differential requirements for cytokinin and CYCD3 activity
Visit the PubMed ArticleARR6 ... transcripts were not up-regulated in STMoe/CKX1oe plants
Scofield S, Dewitte W, Nieuwland J, Murray JA - The Arabidopsis homeobox gene SHOOT MERISTEMLESS has cellular and meristem-organisational roles with differential requirements for cytokinin and CYCD3 activity
Visit the PubMed Articleconversely found no increase in STM mRNA in ... KNAT1oe
Scofield S, Dewitte W, Nieuwland J, Murray JA - The Arabidopsis homeobox gene SHOOT MERISTEMLESS has cellular and meristem-organisational roles with differential requirements for cytokinin and CYCD3 activity
Visit the PubMed ArticleInterestingly, ATHB2:GUS expression was enhanced in the apical side of early heart hat3-3 athb4-1 embryos with respect to wild-type ones (Fig. 3L,N; P=0.0002). Furthermore, ATHB2:GUS was also detected in SAM of several hat3-3 athb4-1 mature embryos
Turchi L, Carabelli M, Ruzza V, Possenti M, Sassi M, Peñalosa A, Sessa G, Salvi S, Forte V, Morelli G, Ruberti I - Arabidopsis HD-Zip II transcription factors control apical embryo development and meristem function
Visit the PubMed Articlehat3-3 athb4-1 ... no significant difference in the expression of ... CLAVATA3 (CLV3 ... By contrast, hat3-3 athb4-1 seedlings with fused/single cotyledon(s) could be split into two groups, one with active SAM and the other one with inactive SAM, characterized by the absence of any cell dome in the site of presumptive SAM (supplementary material Fig. S11). The former group expresses ... CLV3::GUS whereas the latter one does not
Turchi L, Carabelli M, Ruzza V, Possenti M, Sassi M, Peñalosa A, Sessa G, Salvi S, Forte V, Morelli G, Ruberti I - Arabidopsis HD-Zip II transcription factors control apical embryo development and meristem function
Visit the PubMed Articlehat3-3 athb4-1 ... no significant difference in the expression of WUSCHEL (WUS ... By contrast, hat3-3 athb4-1 seedlings with fused/single cotyledon(s) could be split into two groups, one with active SAM and the other one with inactive SAM, characterized by the absence of any cell dome in the site of presumptive SAM (supplementary material Fig. S11). The former group expresses WUS::GUS ... whereas the latter one does not
Turchi L, Carabelli M, Ruzza V, Possenti M, Sassi M, Peñalosa A, Sessa G, Salvi S, Forte V, Morelli G, Ruberti I - Arabidopsis HD-Zip II transcription factors control apical embryo development and meristem function
Visit the PubMed ArticleTo this end, we analyzed YAB3 expression in hat3-3 athb4-1 using the yab3-2 gene trap line, which faithfully reproduces YABBY3 (YAB3) expression in leaves (YAB3::GUS) (Kumaran et al., 2002). GUS activity was detected uniformly throughout first leaf primordia in a significant number of hat3-3 athb4-1 YAB3::GUS/+ descendants whereas it was restricted to the abaxial domain of the young lateral organs of YAB3::GUS plants (Fig. 6G,H; P=0.0001). The expressivity of the phenotype in the first leaves of hat3-3 athb4-1 plants is variable, and it seems likely that the observed uniform expression of YAB3::GUS is associated with radialized primordia. Consistent with this, YAB3::GUS was expressed both in the adaxial and abaxial regions of the third and fourth leaf primordia of almost all the hat3-3 athb4-1 YAB3::GUS/+ descendants with detectable β-glucuronidase activity (56/61) whereas it was restricted to the abaxial domain in YAB3::GUS plants (58/58
Turchi L, Carabelli M, Ruzza V, Possenti M, Sassi M, Peñalosa A, Sessa G, Salvi S, Forte V, Morelli G, Ruberti I - Arabidopsis HD-Zip II transcription factors control apical embryo development and meristem function
Visit the PubMed ArticleWe then assessed whether ATHB2:GUS expression attenuates the leaf phenotype of hat3-3 athb4-1. Leaf development defects in hat3-3 athb4-1 ATHB2::ATHB2:GUS were significantly less severe than those of hat3-3 athb4-1 (Fig. 6I,J; supplementary material Fig. S13C). In ATHB2::ATHB2:GUS leaf primordia, GUS activity was detected exclusively in provascular cells. By contrast, a significant number of hat3-3 athb4-1 plants also express ATHB2:GUS in the adaxial domain of leaf primordia
Turchi L, Carabelli M, Ruzza V, Possenti M, Sassi M, Peñalosa A, Sessa G, Salvi S, Forte V, Morelli G, Ruberti I - Arabidopsis HD-Zip II transcription factors control apical embryo development and meristem function
Visit the PubMed ArticlemRNA levels of ATHB2 were reduced after 4 hours of β-estradiol-induced HAT3 expression
Turchi L, Carabelli M, Ruzza V, Possenti M, Sassi M, Peñalosa A, Sessa G, Salvi S, Forte V, Morelli G, Ruberti I - Arabidopsis HD-Zip II transcription factors control apical embryo development and meristem function
Visit the PubMed ArticleATHB4 is mainly expressed in the apical domain of globular and transition stage embryos. By the heart stage, ATHB4 expression resembles that of HAT3
Turchi L, Carabelli M, Ruzza V, Possenti M, Sassi M, Peñalosa A, Sessa G, Salvi S, Forte V, Morelli G, Ruberti I - Arabidopsis HD-Zip II transcription factors control apical embryo development and meristem function
Visit the PubMed ArticleHAT3::GUS ... expression in leaf primordia is restricted to the adaxial domain
Turchi L, Carabelli M, Ruzza V, Possenti M, Sassi M, Peñalosa A, Sessa G, Salvi S, Forte V, Morelli G, Ruberti I - Arabidopsis HD-Zip II transcription factors control apical embryo development and meristem function
Visit the PubMed ArticleHigher ATHB2 mRNA levels have also been observed in DEX-treated 35S::FLAG-GR-REVd plants
Turchi L, Carabelli M, Ruzza V, Possenti M, Sassi M, Peñalosa A, Sessa G, Salvi S, Forte V, Morelli G, Ruberti I - Arabidopsis HD-Zip II transcription factors control apical embryo development and meristem function
Visit the PubMed ArticleAt the early globular stage, HAT3 is expressed in the embryo proper and excluded from the hypophysis. By the heart stage, the expression domain of HAT3 is mostly restricted to the apical part of the embryo, marking the incipient cotyledons. At the torpedo stage, HAT3 is mainly expressed in the adaxial side of the cotyledons and in the SAM. In mature embryos, HAT3 marks the SAM and the provascular system
Turchi L, Carabelli M, Ruzza V, Possenti M, Sassi M, Peñalosa A, Sessa G, Salvi S, Forte V, Morelli G, Ruberti I - Arabidopsis HD-Zip II transcription factors control apical embryo development and meristem function
Visit the PubMed ArticleIn wt embryos, ATHB2:GUS is localized in provascular cells from the early globular stage throughout all developmental stages (Fig. 3K-M), as ATHB2 RNA. There are, however, some differences in ATHB2:GUS expression pattern compared with that of ATHB2. Weak expression of ATHB2:GUS was detected in the apical side of heart embryos, but no GUS signal was seen in SAM at late stages of embryo development (Fig. 3L,M). This might be due to the absence of one or more regulatory elements in the chimeric gene or might be the consequence of additional regulation at the translational and/or post-translational level
Turchi L, Carabelli M, Ruzza V, Possenti M, Sassi M, Peñalosa A, Sessa G, Salvi S, Forte V, Morelli G, Ruberti I - Arabidopsis HD-Zip II transcription factors control apical embryo development and meristem function
Visit the PubMed ArticleATHB2 mRNA levels were reduced in rev-5
Turchi L, Carabelli M, Ruzza V, Possenti M, Sassi M, Peñalosa A, Sessa G, Salvi S, Forte V, Morelli G, Ruberti I - Arabidopsis HD-Zip II transcription factors control apical embryo development and meristem function
Visit the PubMed ArticleIn situ hybridization revealed specific expression pattern of ATHB2 during embryogenesis (Fig. 3A-C; supplementary material Fig. S7). At the globular stage, ATHB2 is expressed in the procambial cells. The vascular expression is maintained throughout all developmental stages. By the late torpedo stage until maturation, ATHB2 is also expressed in RAM. A weak expression of ATHB2 is also detected in SAM of mature embryos
Turchi L, Carabelli M, Ruzza V, Possenti M, Sassi M, Peñalosa A, Sessa G, Salvi S, Forte V, Morelli G, Ruberti I - Arabidopsis HD-Zip II transcription factors control apical embryo development and meristem function
Visit the PubMed ArticleATHB2 mRNA levels were ... increased after 4 hours of β-estradiol-induced ATHB8d expression
Turchi L, Carabelli M, Ruzza V, Possenti M, Sassi M, Peñalosa A, Sessa G, Salvi S, Forte V, Morelli G, Ruberti I - Arabidopsis HD-Zip II transcription factors control apical embryo development and meristem function
Visit the PubMed ArticleTo investigate further the links between auxin response and distribution and HAT3 and ATHB4 function, hat3-3 athb4-1 was crossed with PIN1::PIN1:GFP (Benková et al., 2003). Remarkably, PIN1:GFP expression in mature mutant embryos (Fig. 2H,L,P) closely mirrors the vascular defects observed in hat3-3 athb4-1 seedlings (Fig. 2F,J,N; supplementary material Fig. S4A). No significant difference was observed in PIN1:GFP expression in hypocotyls and roots of embryos altered in cotyledon development relative to wt
Turchi L, Carabelli M, Ruzza V, Possenti M, Sassi M, Peñalosa A, Sessa G, Salvi S, Forte V, Morelli G, Ruberti I - Arabidopsis HD-Zip II transcription factors control apical embryo development and meristem function
Visit the PubMed ArticleAuxin has a key role in both initiation and development of cotyledons during embryogenesis (Chandler, 2008). To investigate whether HAT3 and ATHB4 interfere with auxin distribution and response, hat3-3 athb4-1 was crossed with DR5rev::GFP (Friml et al., 2003). Mature embryos with two cotyledons differing in their degree of expansion and those with fused/single cotyledon(s) were selected to determine DR5rev::GFP pattern. Among the former, consistent with the phenotypes observed in hat3-3 athb4-1 seedlings, the most frequently seen were those with one cotyledon moderately expanded and one more significantly impaired in expansion (Fig. 2E,F; supplementary material Fig. S4A; data not shown). No significant difference was observed in DR5rev::GFP maximum in the root tip in hat3-3 athb4-1 embryos relative to Col-0. By contrast, GFP signal was significantly reduced or almost undetectable in cotyledons of mutant embryos (Fig. 2C,G). Remarkably, DR5rev::GFP expression strictly correlated with the degree of cotyledon expansion (Fig. 2G; data not shown). A significant number of hat3-3 athb4-1 seedlings displayed fused/single cotyledon(s) (Fig. 2I,J,M,N; supplementary material Fig. S4A). hat3-3 athb4-1 embryos with fused/single expanded cotyledon(s) display either two very close DR5rev::GFP maxima (Fig. 2K) or a single DR5rev::GFP maximum (Fig. 2O).
Turchi L, Carabelli M, Ruzza V, Possenti M, Sassi M, Peñalosa A, Sessa G, Salvi S, Forte V, Morelli G, Ruberti I - Arabidopsis HD-Zip II transcription factors control apical embryo development and meristem function
Visit the PubMed ArticleATHB2 mRNA levels were reduced in ... phv-11 phb-13
Turchi L, Carabelli M, Ruzza V, Possenti M, Sassi M, Peñalosa A, Sessa G, Salvi S, Forte V, Morelli G, Ruberti I - Arabidopsis HD-Zip II transcription factors control apical embryo development and meristem function
Visit the PubMed ArticleATHB4::GUS expression in leaf primordia is restricted to the adaxial domain
Turchi L, Carabelli M, Ruzza V, Possenti M, Sassi M, Peñalosa A, Sessa G, Salvi S, Forte V, Morelli G, Ruberti I - Arabidopsis HD-Zip II transcription factors control apical embryo development and meristem function
Visit the PubMed Articlegrown at warm temperature (22°C ... in ... camta2 ... CBP60g ... transcript levels ... were greater
Kim Y, Park S, Gilmour SJ, Thomashow MF - Roles of CAMTA transcription factors and salicylic acid in configuring the low-temperature transcriptome and freezing tolerance of Arabidopsis
Visit the PubMed Articlereduced ... cold induction of ... CBF3 ... in ... camta1/3 double mutant plants
Kim Y, Park S, Gilmour SJ, Thomashow MF - Roles of CAMTA transcription factors and salicylic acid in configuring the low-temperature transcriptome and freezing tolerance of Arabidopsis
Visit the PubMed Articlereduced ... cold induction of CBF1 ... in ... camta2/3 double mutant plants
Kim Y, Park S, Gilmour SJ, Thomashow MF - Roles of CAMTA transcription factors and salicylic acid in configuring the low-temperature transcriptome and freezing tolerance of Arabidopsis
Visit the PubMed Articlegrown at warm temperature (22°C ... in ... camta1 camta3 ... ICS1 ... transcript levels ... were greater
Kim Y, Park S, Gilmour SJ, Thomashow MF - Roles of CAMTA transcription factors and salicylic acid in configuring the low-temperature transcriptome and freezing tolerance of Arabidopsis
Visit the PubMed ArticleSARD1 ... increased between 1 and 2 weeks after the start of cold treatment
Kim Y, Park S, Gilmour SJ, Thomashow MF - Roles of CAMTA transcription factors and salicylic acid in configuring the low-temperature transcriptome and freezing tolerance of Arabidopsis
Visit the PubMed Articlegrown at warm temperature (22°C ... in ... camta1 camta2 camta3 ... ICS1 ... transcript levels ... were greater
Kim Y, Park S, Gilmour SJ, Thomashow MF - Roles of CAMTA transcription factors and salicylic acid in configuring the low-temperature transcriptome and freezing tolerance of Arabidopsis
Visit the PubMed Articlereduced ... cold induction of ... CBF2 ... in ... camta1/2 ... double mutant plants
Kim Y, Park S, Gilmour SJ, Thomashow MF - Roles of CAMTA transcription factors and salicylic acid in configuring the low-temperature transcriptome and freezing tolerance of Arabidopsis
Visit the PubMed Articlegrown at warm temperature (22°C ... in ... camta3 ... SARD1 ... transcript levels ... were greater
Kim Y, Park S, Gilmour SJ, Thomashow MF - Roles of CAMTA transcription factors and salicylic acid in configuring the low-temperature transcriptome and freezing tolerance of Arabidopsis
Visit the PubMed Articlegrown at warm temperature (22°C ... in ... camta1 ... ICS1 ... transcript levels ... were greater
Kim Y, Park S, Gilmour SJ, Thomashow MF - Roles of CAMTA transcription factors and salicylic acid in configuring the low-temperature transcriptome and freezing tolerance of Arabidopsis
Visit the PubMed Articlegrown at warm temperature (22°C ... in ... camta1 camta2 camta3 ... SARD1 ... transcript levels ... were greater
Kim Y, Park S, Gilmour SJ, Thomashow MF - Roles of CAMTA transcription factors and salicylic acid in configuring the low-temperature transcriptome and freezing tolerance of Arabidopsis
Visit the PubMed Articlegrown at warm temperature (22°C ... in ... camta3 ... ICS1 ... transcript levels ... were greater
Kim Y, Park S, Gilmour SJ, Thomashow MF - Roles of CAMTA transcription factors and salicylic acid in configuring the low-temperature transcriptome and freezing tolerance of Arabidopsis
Visit the PubMed Articlereduced ... cold induction of ... CBF2 ... in ... camta2/3 double mutant plants
Kim Y, Park S, Gilmour SJ, Thomashow MF - Roles of CAMTA transcription factors and salicylic acid in configuring the low-temperature transcriptome and freezing tolerance of Arabidopsis
Visit the PubMed Articlegrown at warm temperature (22°C ... in ... camta1 ... CBP60g ... transcript levels ... were greater
Kim Y, Park S, Gilmour SJ, Thomashow MF - Roles of CAMTA transcription factors and salicylic acid in configuring the low-temperature transcriptome and freezing tolerance of Arabidopsis
Visit the PubMed Articlecamta1/3 double mutation ... had little effect on cold induction of CBF2
Kim Y, Park S, Gilmour SJ, Thomashow MF - Roles of CAMTA transcription factors and salicylic acid in configuring the low-temperature transcriptome and freezing tolerance of Arabidopsis
Visit the PubMed Articlereduced ... cold induction of ... CBF3 ... in ... camta2/3 double mutant plants
Kim Y, Park S, Gilmour SJ, Thomashow MF - Roles of CAMTA transcription factors and salicylic acid in configuring the low-temperature transcriptome and freezing tolerance of Arabidopsis
Visit the PubMed Articlegrown at warm temperature (22°C ... in ... camta2 ... SARD1 ... transcript levels ... were greater
Kim Y, Park S, Gilmour SJ, Thomashow MF - Roles of CAMTA transcription factors and salicylic acid in configuring the low-temperature transcriptome and freezing tolerance of Arabidopsis
Visit the PubMed Articlethe transcript levels for ... CAMTA2 did not change appreciably during exposure of plants to low temperature for 5 weeks
Kim Y, Park S, Gilmour SJ, Thomashow MF - Roles of CAMTA transcription factors and salicylic acid in configuring the low-temperature transcriptome and freezing tolerance of Arabidopsis
Visit the PubMed Articlegrown at warm temperature (22°C ... in ... camta1 camta2 camta3 ... CBP60g ... transcript levels ... were greater
Kim Y, Park S, Gilmour SJ, Thomashow MF - Roles of CAMTA transcription factors and salicylic acid in configuring the low-temperature transcriptome and freezing tolerance of Arabidopsis
Visit the PubMed Articlereduced ... cold induction of CBF1 ... in ... camta1/2 ... double mutant plants
Kim Y, Park S, Gilmour SJ, Thomashow MF - Roles of CAMTA transcription factors and salicylic acid in configuring the low-temperature transcriptome and freezing tolerance of Arabidopsis
Visit the PubMed Articlethe transcript levels for CAMTA1 ... did not change appreciably during exposure of plants to low temperature for 5 weeks
Kim Y, Park S, Gilmour SJ, Thomashow MF - Roles of CAMTA transcription factors and salicylic acid in configuring the low-temperature transcriptome and freezing tolerance of Arabidopsis
Visit the PubMed Articlecamta1/3 double mutation resulted in impaired cold induction of CBF1
Kim Y, Park S, Gilmour SJ, Thomashow MF - Roles of CAMTA transcription factors and salicylic acid in configuring the low-temperature transcriptome and freezing tolerance of Arabidopsis
Visit the PubMed Articlegrown at warm temperature (22°C ... in ... camta2 camta3 ... ICS1 ... transcript levels ... were greater
Kim Y, Park S, Gilmour SJ, Thomashow MF - Roles of CAMTA transcription factors and salicylic acid in configuring the low-temperature transcriptome and freezing tolerance of Arabidopsis
Visit the PubMed Articlegrown at warm temperature (22°C ... in ... camta2 camta3 ... SARD1 ... transcript levels ... were greater
Kim Y, Park S, Gilmour SJ, Thomashow MF - Roles of CAMTA transcription factors and salicylic acid in configuring the low-temperature transcriptome and freezing tolerance of Arabidopsis
Visit the PubMed Articlegrown at warm temperature (22°C ... in ... camta1 camta3 ... SARD1 ... transcript levels ... were greater
Kim Y, Park S, Gilmour SJ, Thomashow MF - Roles of CAMTA transcription factors and salicylic acid in configuring the low-temperature transcriptome and freezing tolerance of Arabidopsis
Visit the PubMed Articleduring exposure of plants to low temperature for 5 weeks ... transcript levels for CAMTA3 increased
Kim Y, Park S, Gilmour SJ, Thomashow MF - Roles of CAMTA transcription factors and salicylic acid in configuring the low-temperature transcriptome and freezing tolerance of Arabidopsis
Visit the PubMed ArticleICS1 transcript levels increased dramatically between 1 and 2 weeks after the start of exposure of plants to low temperature
Kim Y, Park S, Gilmour SJ, Thomashow MF - Roles of CAMTA transcription factors and salicylic acid in configuring the low-temperature transcriptome and freezing tolerance of Arabidopsis
Visit the PubMed Articlereduced ... cold induction of ... CBF3 ... in ... camta1/2 ... double mutant plants
Kim Y, Park S, Gilmour SJ, Thomashow MF - Roles of CAMTA transcription factors and salicylic acid in configuring the low-temperature transcriptome and freezing tolerance of Arabidopsis
Visit the PubMed Articlegrown at warm temperature (22°C ... in ... camta2 camta3 ... CBP60g ... transcript levels ... were greater
Kim Y, Park S, Gilmour SJ, Thomashow MF - Roles of CAMTA transcription factors and salicylic acid in configuring the low-temperature transcriptome and freezing tolerance of Arabidopsis
Visit the PubMed Articlegrown at warm temperature (22°C ... in ... camta1 ... SARD1 ... transcript levels ... were greater
Kim Y, Park S, Gilmour SJ, Thomashow MF - Roles of CAMTA transcription factors and salicylic acid in configuring the low-temperature transcriptome and freezing tolerance of Arabidopsis
Visit the PubMed ArticleCBP60g ... increased between 1 and 2 weeks after the start of cold treatment
Kim Y, Park S, Gilmour SJ, Thomashow MF - Roles of CAMTA transcription factors and salicylic acid in configuring the low-temperature transcriptome and freezing tolerance of Arabidopsis
Visit the PubMed Articlegrown at warm temperature (22°C ... in ... camta1 camta3 ... CBP60g ... transcript levels ... were greater
Kim Y, Park S, Gilmour SJ, Thomashow MF - Roles of CAMTA transcription factors and salicylic acid in configuring the low-temperature transcriptome and freezing tolerance of Arabidopsis
Visit the PubMed Articlegrown at warm temperature (22°C ... in ... camta3 ... CBP60g ... transcript levels ... were greater
Kim Y, Park S, Gilmour SJ, Thomashow MF - Roles of CAMTA transcription factors and salicylic acid in configuring the low-temperature transcriptome and freezing tolerance of Arabidopsis
Visit the PubMed Articleat warm temperature (22°C ... in ... camta2 ... ICS1 ... transcript levels ... levels ... were greater
Kim Y, Park S, Gilmour SJ, Thomashow MF - Roles of CAMTA transcription factors and salicylic acid in configuring the low-temperature transcriptome and freezing tolerance of Arabidopsis
Visit the PubMed Articlereduced ... cold induction of ... CBF2 ... in ... camta1/3 double mutant plants
Kim Y, Park S, Gilmour SJ, Thomashow MF - Roles of CAMTA transcription factors and salicylic acid in configuring the low-temperature transcriptome and freezing tolerance of Arabidopsis
Visit the PubMed ArticleThe main–1 mutant was identified in a genetic screen for altered GFP distribution in Arabidopsis root tips. T–DNA mutagenesis was performed using an Arabidopsis line (ecotype C24) that expressed GFP from the companion cell-specific Arabidopsis SUCROSE TRANSPORTER2 promoter (pSUC2::GFP), in which GFP moves into sieve elements, is translocated to sinks and is unloaded symplastically from the phloem ends ... were unable to unload GFP from the root vasculature
Wenig U, Meyer S, Stadler R, Fischer S, Werner D, Lauter A, Melzer M, Hoth S, Weingartner M, Sauer N - Identification of MAIN, a factor involved in genome stability in the meristems of Arabidopsis thaliana
Visit the PubMed ArticleWe analysed the unloading phenotype of main–2 by crossing main–2 plants (ecotype Columbia, Col–0) with wild-type (WT) plants (Col–0) that expressed GFP under the control of the SUC2 promoter (Figure 1c) (Schneidereit et al., 2008). In this background, main–2 showed an identical phenotype (Figure 1d) to main–1 (Figure 1b
Wenig U, Meyer S, Stadler R, Fischer S, Werner D, Lauter A, Melzer M, Hoth S, Weingartner M, Sauer N - Identification of MAIN, a factor involved in genome stability in the meristems of Arabidopsis thaliana
Visit the PubMed ArticleWe also monitored the expression pattern of the DR5::GFP reporter after incubation of seedlings with auxin or with auxin inhibitors, and obtained similar results in the main mutant background and in WT
Wenig U, Meyer S, Stadler R, Fischer S, Werner D, Lauter A, Melzer M, Hoth S, Weingartner M, Sauer N - Identification of MAIN, a factor involved in genome stability in the meristems of Arabidopsis thaliana
Visit the PubMed ArticleStrong pMAIN::MAIN-GUS expression was also observed in the SAM
Wenig U, Meyer S, Stadler R, Fischer S, Werner D, Lauter A, Melzer M, Hoth S, Weingartner M, Sauer N - Identification of MAIN, a factor involved in genome stability in the meristems of Arabidopsis thaliana
Visit the PubMed ArticleAnalysis of pMAIN::MAIN-GFP lines revealed that the MAIN–GFP fusion protein accumulated in all cells of the embryo at all developmental stages
Wenig U, Meyer S, Stadler R, Fischer S, Werner D, Lauter A, Melzer M, Hoth S, Weingartner M, Sauer N - Identification of MAIN, a factor involved in genome stability in the meristems of Arabidopsis thaliana
Visit the PubMed Articlewe fused GFP to the MAIN cDNA under the control of the CaMV 35S promoter (p35S::MAIN-GFP). This construct was used for particle bombardment of tobacco (Nicotiana tabacum) epidermis cells, and the results showed that the fusion protein localized exclusively to the nucleus
Wenig U, Meyer S, Stadler R, Fischer S, Werner D, Lauter A, Melzer M, Hoth S, Weingartner M, Sauer N - Identification of MAIN, a factor involved in genome stability in the meristems of Arabidopsis thaliana
Visit the PubMed ArticleTo investigate whether MAIN is involved in auxin signalling, we monitored the expression of primary auxin response genes such as INDOLE-3–ACETIC ACID INDUCIBLE1 (IAA1 ... in seedlings at 3 DAG that had been incubated for 2 h on medium containing 10 µm auxin. The main-2 mutants behaved like WT seedlings ... induced after auxin treatment
Wenig U, Meyer S, Stadler R, Fischer S, Werner D, Lauter A, Melzer M, Hoth S, Weingartner M, Sauer N - Identification of MAIN, a factor involved in genome stability in the meristems of Arabidopsis thaliana
Visit the PubMed ArticleA construct in which GFP was fused to the 5' end of MAIN and particle-bombarded into epidermis cells of Arabidopsis, tobacco and onion (Allium cepa) confirmed the localization to the nucleus
Wenig U, Meyer S, Stadler R, Fischer S, Werner D, Lauter A, Melzer M, Hoth S, Weingartner M, Sauer N - Identification of MAIN, a factor involved in genome stability in the meristems of Arabidopsis thaliana
Visit the PubMed Articlethe expression of MAIN was not changed in WT seedlings upon addition of auxin
Wenig U, Meyer S, Stadler R, Fischer S, Werner D, Lauter A, Melzer M, Hoth S, Weingartner M, Sauer N - Identification of MAIN, a factor involved in genome stability in the meristems of Arabidopsis thaliana
Visit the PubMed Articlethe complete MAIN genomic region, including the promoter region that was used for the complementation construct, fused to the uidA (GUS) gene of Escherichia coli (pMAIN::MAIN-GUS) or GFP (pMAIN::MAIN-GFP ... In general, MAIN was most strongly expressed in all meristematic tissues, i.e. the SAM
Wenig U, Meyer S, Stadler R, Fischer S, Werner D, Lauter A, Melzer M, Hoth S, Weingartner M, Sauer N - Identification of MAIN, a factor involved in genome stability in the meristems of Arabidopsis thaliana
Visit the PubMed ArticleIn the aerial parts, pMAIN::MAIN-GUS staining was generally weaker ... was mainly detected in the vasculature of leaves at all developmental stages
Wenig U, Meyer S, Stadler R, Fischer S, Werner D, Lauter A, Melzer M, Hoth S, Weingartner M, Sauer N - Identification of MAIN, a factor involved in genome stability in the meristems of Arabidopsis thaliana
Visit the PubMed ArticleTo investigate whether MAIN is involved in auxin signalling, we monitored the expression of primary auxin response genes such as ... IAA19 in seedlings at 3 DAG that had been incubated for 2 h on medium containing 10 µm auxin. The main-2 mutants behaved like WT seedlings ... induced after auxin treatment
Wenig U, Meyer S, Stadler R, Fischer S, Werner D, Lauter A, Melzer M, Hoth S, Weingartner M, Sauer N - Identification of MAIN, a factor involved in genome stability in the meristems of Arabidopsis thaliana
Visit the PubMed ArticleNULL
Wenig U, Meyer S, Stadler R, Fischer S, Werner D, Lauter A, Melzer M, Hoth S, Weingartner M, Sauer N - Identification of MAIN, a factor involved in genome stability in the meristems of Arabidopsis thaliana
Visit the PubMed ArticleAtKRP6 over-expression ... higher mRNA levels for ... AtCycD4.1
Guérinier T, Millan L, Crozet P, Oury C, Rey F, Valot B, Mathieu C, Vidal J, Hodges M, Thomas M, Glab N - Phosphorylation of p27(KIP1) homologs KRP6 and 7 by SNF1-related protein kinase-1 links plant energy homeostasis and cell proliferation
Visit the PubMed ArticleTo investigate the effect of AtKRP6 phosphorylation on its subcellular localization, chimeric AtKRP6–GFP proteins (containing AtKRP6WT, AtKRP6T152A or AtKRP6T152D) were transiently expressed in A. thaliana protoplasts. Both AtKRP6T152A and AtKRP6T152D showed nuclear localization like the WT form. Thus, it appears that, under our conditions, Thr152 phosphorylation alone does not lead to re-localization of AtKRP6–GFP to the cytoplasm
Guérinier T, Millan L, Crozet P, Oury C, Rey F, Valot B, Mathieu C, Vidal J, Hodges M, Thomas M, Glab N - Phosphorylation of p27(KIP1) homologs KRP6 and 7 by SNF1-related protein kinase-1 links plant energy homeostasis and cell proliferation
Visit the PubMed ArticleAtKRP6 over-expression ... higher mRNA levels for ... AtCycD3.1
Guérinier T, Millan L, Crozet P, Oury C, Rey F, Valot B, Mathieu C, Vidal J, Hodges M, Thomas M, Glab N - Phosphorylation of p27(KIP1) homologs KRP6 and 7 by SNF1-related protein kinase-1 links plant energy homeostasis and cell proliferation
Visit the PubMed Articleick1/2 ... we analyzed the CDKA;1 protein level in these mutants and controls by western blotting using an antibody against the conserved PSTAIRE domain of Cdc2 kinase. As shown in Figure S3, the CDKA;1 protein level was relatively constant among the mutants and controls
Cheng Y, Cao L, Wang S, Li Y, Shi X, Liu H, Li L, Zhang Z, Fowke LC, Wang H, Zhou Y - Downregulation of multiple CDK inhibitor ICK/KRP genes upregulates the E2F pathway and increases cell proliferation, and organ and seed sizes in Arabidopsis
Visit the PubMed Articleick6/7 ... we analyzed the CDKA;1 protein level in these mutants and controls by western blotting using an antibody against the conserved PSTAIRE domain of Cdc2 kinase. As shown in Figure S3, the CDKA;1 protein level was relatively constant among the mutants and controls
Cheng Y, Cao L, Wang S, Li Y, Shi X, Liu H, Li L, Zhang Z, Fowke LC, Wang H, Zhou Y - Downregulation of multiple CDK inhibitor ICK/KRP genes upregulates the E2F pathway and increases cell proliferation, and organ and seed sizes in Arabidopsis
Visit the PubMed Articleick1/2/5/6/7 ... RBR1 protein level increased in the ... quintuple mutants compared with the Wt controls ... Accordingly, we further analyzed the level of phosphorylated RBR1 in the quintuple mutant with an antibody that has been used in other studies against plant phospho-Rb proteins (Ábrahám et al., 2011; Polit et al., 2012). The results from independent experiments showed that phospho-RBR1 level also increased
Cheng Y, Cao L, Wang S, Li Y, Shi X, Liu H, Li L, Zhang Z, Fowke LC, Wang H, Zhou Y - Downregulation of multiple CDK inhibitor ICK/KRP genes upregulates the E2F pathway and increases cell proliferation, and organ and seed sizes in Arabidopsis
Visit the PubMed Articleick2 ... we analyzed the CDKA;1 protein level in these mutants and controls by western blotting using an antibody against the conserved PSTAIRE domain of Cdc2 kinase. As shown in Figure S3, the CDKA;1 protein level was relatively constant among the mutants and controls
Cheng Y, Cao L, Wang S, Li Y, Shi X, Liu H, Li L, Zhang Z, Fowke LC, Wang H, Zhou Y - Downregulation of multiple CDK inhibitor ICK/KRP genes upregulates the E2F pathway and increases cell proliferation, and organ and seed sizes in Arabidopsis
Visit the PubMed Articleick1/2/5/6/7 quintuple mutant ... For the 30 cyclin genes analyzed, most showed little change, but five mitotic cyclin genes were upregulated with a log2 value of more than 0.25, whereas three D-type cyclin genes showed downregulation, with a log2 value of lower than -0.25
Cheng Y, Cao L, Wang S, Li Y, Shi X, Liu H, Li L, Zhang Z, Fowke LC, Wang H, Zhou Y - Downregulation of multiple CDK inhibitor ICK/KRP genes upregulates the E2F pathway and increases cell proliferation, and organ and seed sizes in Arabidopsis
Visit the PubMed Articleick7 ... we analyzed the CDKA;1 protein level in these mutants and controls by western blotting using an antibody against the conserved PSTAIRE domain of Cdc2 kinase. As shown in Figure S3, the CDKA;1 protein level was relatively constant among the mutants and controls
Cheng Y, Cao L, Wang S, Li Y, Shi X, Liu H, Li L, Zhang Z, Fowke LC, Wang H, Zhou Y - Downregulation of multiple CDK inhibitor ICK/KRP genes upregulates the E2F pathway and increases cell proliferation, and organ and seed sizes in Arabidopsis
Visit the PubMed Articleick1/2/5/6/7 ... we analyzed the CDKA;1 protein level in these mutants and controls by western blotting using an antibody against the conserved PSTAIRE domain of Cdc2 kinase. As shown in Figure S3, the CDKA;1 protein level was relatively constant among the mutants and controls
Cheng Y, Cao L, Wang S, Li Y, Shi X, Liu H, Li L, Zhang Z, Fowke LC, Wang H, Zhou Y - Downregulation of multiple CDK inhibitor ICK/KRP genes upregulates the E2F pathway and increases cell proliferation, and organ and seed sizes in Arabidopsis
Visit the PubMed Articleick1/2/5/6/7 quintuple mutant ... There were slight increases in the levels of ICK3 ... transcripts
Cheng Y, Cao L, Wang S, Li Y, Shi X, Liu H, Li L, Zhang Z, Fowke LC, Wang H, Zhou Y - Downregulation of multiple CDK inhibitor ICK/KRP genes upregulates the E2F pathway and increases cell proliferation, and organ and seed sizes in Arabidopsis
Visit the PubMed Articleick1/2/5/6/7 quintuple mutant ... Among the 12 CDK genes, all showed a trend of upregulation, with six of them having a log2 value (log2 of relative expression) more than 0.25 (Figure S7a). This trend is more obvious when the transcript levels of CDKs are viewed as a group. However, the extent of upregulation for each CDK was limited, with the CDKA;1 transcript level in the quintuple mutant being 1.15-fold that in the Wt (Figure S7a). In this regard, the results from western analysis indicate that there was no difference between Wt and quintuple mutant plants in the level of CDAK protein (Figure S3). This slight difference is understandable, as an increase in transcripts does not automatically translate into an exact increase in the protein level
Cheng Y, Cao L, Wang S, Li Y, Shi X, Liu H, Li L, Zhang Z, Fowke LC, Wang H, Zhou Y - Downregulation of multiple CDK inhibitor ICK/KRP genes upregulates the E2F pathway and increases cell proliferation, and organ and seed sizes in Arabidopsis
Visit the PubMed Articleick5 ... we analyzed the CDKA;1 protein level in these mutants and controls by western blotting using an antibody against the conserved PSTAIRE domain of Cdc2 kinase. As shown in Figure S3, the CDKA;1 protein level was relatively constant among the mutants and controls
Cheng Y, Cao L, Wang S, Li Y, Shi X, Liu H, Li L, Zhang Z, Fowke LC, Wang H, Zhou Y - Downregulation of multiple CDK inhibitor ICK/KRP genes upregulates the E2F pathway and increases cell proliferation, and organ and seed sizes in Arabidopsis
Visit the PubMed Articleick1/2/5/6/7 quintuple mutant ... There were slight increases in the levels of ... ICK4 transcripts
Cheng Y, Cao L, Wang S, Li Y, Shi X, Liu H, Li L, Zhang Z, Fowke LC, Wang H, Zhou Y - Downregulation of multiple CDK inhibitor ICK/KRP genes upregulates the E2F pathway and increases cell proliferation, and organ and seed sizes in Arabidopsis
Visit the PubMed Articleick1/2/6/7 ... we analyzed the CDKA;1 protein level in these mutants and controls by western blotting using an antibody against the conserved PSTAIRE domain of Cdc2 kinase. As shown in Figure S3, the CDKA;1 protein level was relatively constant among the mutants and controls
Cheng Y, Cao L, Wang S, Li Y, Shi X, Liu H, Li L, Zhang Z, Fowke LC, Wang H, Zhou Y - Downregulation of multiple CDK inhibitor ICK/KRP genes upregulates the E2F pathway and increases cell proliferation, and organ and seed sizes in Arabidopsis
Visit the PubMed Articlein the ICK1OE line ... only one gene (At4g20270) was upregulated
Cheng Y, Cao L, Wang S, Li Y, Shi X, Liu H, Li L, Zhang Z, Fowke LC, Wang H, Zhou Y - Downregulation of multiple CDK inhibitor ICK/KRP genes upregulates the E2F pathway and increases cell proliferation, and organ and seed sizes in Arabidopsis
Visit the PubMed Articleick1 ... we analyzed the CDKA;1 protein level in these mutants and controls by western blotting using an antibody against the conserved PSTAIRE domain of Cdc2 kinase. As shown in Figure S3, the CDKA;1 protein level was relatively constant among the mutants and controls
Cheng Y, Cao L, Wang S, Li Y, Shi X, Liu H, Li L, Zhang Z, Fowke LC, Wang H, Zhou Y - Downregulation of multiple CDK inhibitor ICK/KRP genes upregulates the E2F pathway and increases cell proliferation, and organ and seed sizes in Arabidopsis
Visit the PubMed Articleick1/2/6/7 ... RBR1 protein level increased in the quadruple ... compared with the Wt controls ... Accordingly, we further analyzed the level of phosphorylated RBR1 in the quintuple mutant with an antibody that has been used in other studies against plant phospho-Rb proteins (Ábrahám et al., 2011; Polit et al., 2012). The results from independent experiments showed that phospho-RBR1 level also increased
Cheng Y, Cao L, Wang S, Li Y, Shi X, Liu H, Li L, Zhang Z, Fowke LC, Wang H, Zhou Y - Downregulation of multiple CDK inhibitor ICK/KRP genes upregulates the E2F pathway and increases cell proliferation, and organ and seed sizes in Arabidopsis
Visit the PubMed Articleick6 ... we analyzed the CDKA;1 protein level in these mutants and controls by western blotting using an antibody against the conserved PSTAIRE domain of Cdc2 kinase. As shown in Figure S3, the CDKA;1 protein level was relatively constant among the mutants and controls
Cheng Y, Cao L, Wang S, Li Y, Shi X, Liu H, Li L, Zhang Z, Fowke LC, Wang H, Zhou Y - Downregulation of multiple CDK inhibitor ICK/KRP genes upregulates the E2F pathway and increases cell proliferation, and organ and seed sizes in Arabidopsis
Visit the PubMed Articlethere is no apparent difference in PDV1 ... expression between the frs4/cpd25 mutant and the wild type
Gao Y, Liu H, An C, Shi Y, Liu X, Yuan W, Zhang B, Yang J, Yu C, Gao H - Arabidopsis FRS4/CPD25 and FHY3/CPD45 work cooperatively to promote the expression of the chloroplast division gene ARC5 and chloroplast division
Visit the PubMed Articlethe expression level of ARC5 in cpd25 is lower than that of the wild type ... the expression of ARC5 in 20-day-old plate-grown frs4/cpd25 plants was 10.8% of that of wild-type plants and the expression of ARC5 in 40-day-old soil-grown frs4/cpd25 plants was 19.7% of that of wild-type plants
Gao Y, Liu H, An C, Shi Y, Liu X, Yuan W, Zhang B, Yang J, Yu C, Gao H - Arabidopsis FRS4/CPD25 and FHY3/CPD45 work cooperatively to promote the expression of the chloroplast division gene ARC5 and chloroplast division
Visit the PubMed Articletransformation of the fhy3/cpd45 mutant with a 35Spro-CPD25 transgene ... Quantitative RT-PCR data indicated that the level of ARC5 in the transgenic plants was even lower than that in fhy3/cpd45
Gao Y, Liu H, An C, Shi Y, Liu X, Yuan W, Zhang B, Yang J, Yu C, Gao H - Arabidopsis FRS4/CPD25 and FHY3/CPD45 work cooperatively to promote the expression of the chloroplast division gene ARC5 and chloroplast division
Visit the PubMed Articlethere is no apparent difference in ... PDV2 expression between the frs4/cpd25 mutant and the wild type
Gao Y, Liu H, An C, Shi Y, Liu X, Yuan W, Zhang B, Yang J, Yu C, Gao H - Arabidopsis FRS4/CPD25 and FHY3/CPD45 work cooperatively to promote the expression of the chloroplast division gene ARC5 and chloroplast division
Visit the PubMed Articlefrs4/cpd25 mutant was transformed with a 35Spro-CPD45 transgene ... Quantitative RT-PCR analysis indicated that the level of ARC5 in the transgenic plants was recovered to a level even slightly higher than that in the wild type
Gao Y, Liu H, An C, Shi Y, Liu X, Yuan W, Zhang B, Yang J, Yu C, Gao H - Arabidopsis FRS4/CPD25 and FHY3/CPD45 work cooperatively to promote the expression of the chloroplast division gene ARC5 and chloroplast division
Visit the PubMed ArticleGiven the developmental defects observed in ctf7-1 meiocytes the transcript level of highly expressed meiotic genes (Yang et al., 2011b) was measured by QPCR ... significant increases in transcript levels were observed for ... DMC1
Bolaños-Villegas P, Yang X, Wang HJ, Juan CT, Chuang MH, Makaroff CA, Jauh GY - Arabidopsis CHROMOSOME TRANSMISSION FIDELITY 7 (AtCTF7/ECO1) is required for DNA repair, mitosis and meiosis
Visit the PubMed Articlelarge and statistically significant increases (greater than four-fold) in transcript levels were observed for several genes in ctf7-1 plants, including ... BRCA1 (a ubiquitin ligase
Bolaños-Villegas P, Yang X, Wang HJ, Juan CT, Chuang MH, Makaroff CA, Jauh GY - Arabidopsis CHROMOSOME TRANSMISSION FIDELITY 7 (AtCTF7/ECO1) is required for DNA repair, mitosis and meiosis
Visit the PubMed Articlein ctf7-1 plants ... no significant change was observed for ... the M-phase checkpoint genes MAD2
Bolaños-Villegas P, Yang X, Wang HJ, Juan CT, Chuang MH, Makaroff CA, Jauh GY - Arabidopsis CHROMOSOME TRANSMISSION FIDELITY 7 (AtCTF7/ECO1) is required for DNA repair, mitosis and meiosis
Visit the PubMed ArticleGiven the developmental defects observed in ctf7-1 meiocytes the transcript level of highly expressed meiotic genes (Yang et al., 2011b) was measured by QPCR ... significant increases in transcript levels were observed for ... SMC1
Bolaños-Villegas P, Yang X, Wang HJ, Juan CT, Chuang MH, Makaroff CA, Jauh GY - Arabidopsis CHROMOSOME TRANSMISSION FIDELITY 7 (AtCTF7/ECO1) is required for DNA repair, mitosis and meiosis
Visit the PubMed Articlelarge and statistically significant increases (greater than four-fold) in transcript levels were observed for several genes in ctf7-1 plants, including ... PARP2 (a polymerase
Bolaños-Villegas P, Yang X, Wang HJ, Juan CT, Chuang MH, Makaroff CA, Jauh GY - Arabidopsis CHROMOSOME TRANSMISSION FIDELITY 7 (AtCTF7/ECO1) is required for DNA repair, mitosis and meiosis
Visit the PubMed Articlein ctf7-1 plants ... no significant change was observed for ... CDKA1, which regulates the transition from mitosis to endocycle
Bolaños-Villegas P, Yang X, Wang HJ, Juan CT, Chuang MH, Makaroff CA, Jauh GY - Arabidopsis CHROMOSOME TRANSMISSION FIDELITY 7 (AtCTF7/ECO1) is required for DNA repair, mitosis and meiosis
Visit the PubMed Articlein ctf7-1 plants ... Smaller increases (approximately two-fold) were observed for ... SMC6B
Bolaños-Villegas P, Yang X, Wang HJ, Juan CT, Chuang MH, Makaroff CA, Jauh GY - Arabidopsis CHROMOSOME TRANSMISSION FIDELITY 7 (AtCTF7/ECO1) is required for DNA repair, mitosis and meiosis
Visit the PubMed Articlein ctf7-1 plants ... no significant change was observed for ATR (a kinase involved in single-strand break repair
Bolaños-Villegas P, Yang X, Wang HJ, Juan CT, Chuang MH, Makaroff CA, Jauh GY - Arabidopsis CHROMOSOME TRANSMISSION FIDELITY 7 (AtCTF7/ECO1) is required for DNA repair, mitosis and meiosis
Visit the PubMed Articlelarge and statistically significant increases (greater than four-fold) in transcript levels were observed for several genes in ctf7-1 plants, including: ATM (a kinase)
Bolaños-Villegas P, Yang X, Wang HJ, Juan CT, Chuang MH, Makaroff CA, Jauh GY - Arabidopsis CHROMOSOME TRANSMISSION FIDELITY 7 (AtCTF7/ECO1) is required for DNA repair, mitosis and meiosis
Visit the PubMed Articlelarge and statistically significant increases (greater than four-fold) in transcript levels were observed for several genes in ctf7-1 plants, including ... TOPOII-a (a topoisomerase
Bolaños-Villegas P, Yang X, Wang HJ, Juan CT, Chuang MH, Makaroff CA, Jauh GY - Arabidopsis CHROMOSOME TRANSMISSION FIDELITY 7 (AtCTF7/ECO1) is required for DNA repair, mitosis and meiosis
Visit the PubMed Articlein ctf7-1 plants ... Smaller increases (approximately two-fold) were observed for SMC5
Bolaños-Villegas P, Yang X, Wang HJ, Juan CT, Chuang MH, Makaroff CA, Jauh GY - Arabidopsis CHROMOSOME TRANSMISSION FIDELITY 7 (AtCTF7/ECO1) is required for DNA repair, mitosis and meiosis
Visit the PubMed Articlein ctf7-1 plants ... Smaller increases (approximately two-fold) were observed for ... SRS2
Bolaños-Villegas P, Yang X, Wang HJ, Juan CT, Chuang MH, Makaroff CA, Jauh GY - Arabidopsis CHROMOSOME TRANSMISSION FIDELITY 7 (AtCTF7/ECO1) is required for DNA repair, mitosis and meiosis
Visit the PubMed ArticleGiven the developmental defects observed in ctf7-1 meiocytes the transcript level of highly expressed meiotic genes (Yang et al., 2011b) was measured by QPCR ... significant increases in transcript levels were observed for ... BRCA2B
Bolaños-Villegas P, Yang X, Wang HJ, Juan CT, Chuang MH, Makaroff CA, Jauh GY - Arabidopsis CHROMOSOME TRANSMISSION FIDELITY 7 (AtCTF7/ECO1) is required for DNA repair, mitosis and meiosis
Visit the PubMed Articlein ctf7-1 ... No significant increase was observed for the mitotic checkpoint gene BUB1.3
Bolaños-Villegas P, Yang X, Wang HJ, Juan CT, Chuang MH, Makaroff CA, Jauh GY - Arabidopsis CHROMOSOME TRANSMISSION FIDELITY 7 (AtCTF7/ECO1) is required for DNA repair, mitosis and meiosis
Visit the PubMed Articlein ctf7-1 plants ... no significant change was observed for ... NQK1
Bolaños-Villegas P, Yang X, Wang HJ, Juan CT, Chuang MH, Makaroff CA, Jauh GY - Arabidopsis CHROMOSOME TRANSMISSION FIDELITY 7 (AtCTF7/ECO1) is required for DNA repair, mitosis and meiosis
Visit the PubMed ArticleGiven the developmental defects observed in ctf7-1 meiocytes the transcript level of highly expressed meiotic genes (Yang et al., 2011b) was measured by QPCR ... significant increases in transcript levels were observed for ... CDC45, a gene that codes for an S-phase licensing factor and is required for meiosis
Bolaños-Villegas P, Yang X, Wang HJ, Juan CT, Chuang MH, Makaroff CA, Jauh GY - Arabidopsis CHROMOSOME TRANSMISSION FIDELITY 7 (AtCTF7/ECO1) is required for DNA repair, mitosis and meiosis
Visit the PubMed ArticleGiven the developmental defects observed in ctf7-1 meiocytes the transcript level of highly expressed meiotic genes (Yang et al., 2011b) was measured by QPCR ... significant increases in transcript levels were observed for ATM
Bolaños-Villegas P, Yang X, Wang HJ, Juan CT, Chuang MH, Makaroff CA, Jauh GY - Arabidopsis CHROMOSOME TRANSMISSION FIDELITY 7 (AtCTF7/ECO1) is required for DNA repair, mitosis and meiosis
Visit the PubMed ArticleGiven the developmental defects observed in ctf7-1 meiocytes the transcript level of highly expressed meiotic genes (Yang et al., 2011b) was measured by QPCR ... significant increases in transcript levels were observed for ... ATR
Bolaños-Villegas P, Yang X, Wang HJ, Juan CT, Chuang MH, Makaroff CA, Jauh GY - Arabidopsis CHROMOSOME TRANSMISSION FIDELITY 7 (AtCTF7/ECO1) is required for DNA repair, mitosis and meiosis
Visit the PubMed Articlesubcellular localization of AtCTF7 in Arabidopsis protoplasts indicated that AtCTF7 localizes to the nucleus
Bolaños-Villegas P, Yang X, Wang HJ, Juan CT, Chuang MH, Makaroff CA, Jauh GY - Arabidopsis CHROMOSOME TRANSMISSION FIDELITY 7 (AtCTF7/ECO1) is required for DNA repair, mitosis and meiosis
Visit the PubMed Articlelarge and statistically significant increases (greater than four-fold) in transcript levels were observed for several genes in ctf7-1 plants, including ... CYCB1;1 (a G2/M checkpoint gene
Bolaños-Villegas P, Yang X, Wang HJ, Juan CT, Chuang MH, Makaroff CA, Jauh GY - Arabidopsis CHROMOSOME TRANSMISSION FIDELITY 7 (AtCTF7/ECO1) is required for DNA repair, mitosis and meiosis
Visit the PubMed ArticleGiven the developmental defects observed in ctf7-1 meiocytes the transcript level of highly expressed meiotic genes (Yang et al., 2011b) was measured by QPCR ... significant increases in transcript levels were observed for ... RAD51C
Bolaños-Villegas P, Yang X, Wang HJ, Juan CT, Chuang MH, Makaroff CA, Jauh GY - Arabidopsis CHROMOSOME TRANSMISSION FIDELITY 7 (AtCTF7/ECO1) is required for DNA repair, mitosis and meiosis
Visit the PubMed Articlelarge and statistically significant increases (greater than four-fold) in transcript levels were observed for several genes in ctf7-1 plants, including ... RAD51 (a gene involved in homology-dependent DNA repair
Bolaños-Villegas P, Yang X, Wang HJ, Juan CT, Chuang MH, Makaroff CA, Jauh GY - Arabidopsis CHROMOSOME TRANSMISSION FIDELITY 7 (AtCTF7/ECO1) is required for DNA repair, mitosis and meiosis
Visit the PubMed ArticleGiven the developmental defects observed in ctf7-1 meiocytes the transcript level of highly expressed meiotic genes (Yang et al., 2011b) was measured by QPCR ... significant increases in transcript levels were observed for ... SMC3
Bolaños-Villegas P, Yang X, Wang HJ, Juan CT, Chuang MH, Makaroff CA, Jauh GY - Arabidopsis CHROMOSOME TRANSMISSION FIDELITY 7 (AtCTF7/ECO1) is required for DNA repair, mitosis and meiosis
Visit the PubMed ArticleDLK2 ... unresponsive to Z–CL in max2
Scaffidi A, Waters MT, Ghisalberti EL, Dixon KW, Flematti GR, Smith SM - Carlactone-independent seedling morphogenesis in Arabidopsis
Visit the PubMed ArticleDLK2 ... induction by 1 and 10 µm Z–CL was observed in max1–1
Scaffidi A, Waters MT, Ghisalberti EL, Dixon KW, Flematti GR, Smith SM - Carlactone-independent seedling morphogenesis in Arabidopsis
Visit the PubMed ArticleDLK2 ... induction by 1 and 10 µm Z–CL was observed in ... max3–9 mutants
Scaffidi A, Waters MT, Ghisalberti EL, Dixon KW, Flematti GR, Smith SM - Carlactone-independent seedling morphogenesis in Arabidopsis
Visit the PubMed ArticleSTH7 ... in response to 10 µm Z–CL ... showed similar weak responses in wild-type and kai2
Scaffidi A, Waters MT, Ghisalberti EL, Dixon KW, Flematti GR, Smith SM - Carlactone-independent seedling morphogenesis in Arabidopsis
Visit the PubMed ArticleSTH7 ... induction by 1 and 10 µm Z–CL was observed in ... and max3–9 mutants
Scaffidi A, Waters MT, Ghisalberti EL, Dixon KW, Flematti GR, Smith SM - Carlactone-independent seedling morphogenesis in Arabidopsis
Visit the PubMed ArticleDLK2 in response to 10 µm Z–CL ... showed similar weak responses in wild-type and kai2
Scaffidi A, Waters MT, Ghisalberti EL, Dixon KW, Flematti GR, Smith SM - Carlactone-independent seedling morphogenesis in Arabidopsis
Visit the PubMed ArticleSTH7 ... unresponsive to Z–CL in max2
Scaffidi A, Waters MT, Ghisalberti EL, Dixon KW, Flematti GR, Smith SM - Carlactone-independent seedling morphogenesis in Arabidopsis
Visit the PubMed ArticleSTH7 ... in response to 10 µm Z–CL ... had a greatly reduced response in Atd14
Scaffidi A, Waters MT, Ghisalberti EL, Dixon KW, Flematti GR, Smith SM - Carlactone-independent seedling morphogenesis in Arabidopsis
Visit the PubMed ArticleSTH7 ... unresponsive to Z–CL in ... the Atd14 kai2 double mutant
Scaffidi A, Waters MT, Ghisalberti EL, Dixon KW, Flematti GR, Smith SM - Carlactone-independent seedling morphogenesis in Arabidopsis
Visit the PubMed ArticleDLK2 in response to 10 µm Z–CL ... had a greatly reduced response in Atd14
Scaffidi A, Waters MT, Ghisalberti EL, Dixon KW, Flematti GR, Smith SM - Carlactone-independent seedling morphogenesis in Arabidopsis
Visit the PubMed ArticleTreatment of seedlings with 1 µm GR24 increased expression fivefold (STH7) ... whereas 1 µm Z–CL increased expression less than twofold, and even 10 µm Z–CL was only half as active as 1 µm GR24
Scaffidi A, Waters MT, Ghisalberti EL, Dixon KW, Flematti GR, Smith SM - Carlactone-independent seedling morphogenesis in Arabidopsis
Visit the PubMed ArticleDLK2 ... unresponsive to Z–CL in ... the Atd14 kai2 double mutant
Scaffidi A, Waters MT, Ghisalberti EL, Dixon KW, Flematti GR, Smith SM - Carlactone-independent seedling morphogenesis in Arabidopsis
Visit the PubMed ArticleSTH7 ... induction by 1 and 10 µm Z–CL was observed in max1–1
Scaffidi A, Waters MT, Ghisalberti EL, Dixon KW, Flematti GR, Smith SM - Carlactone-independent seedling morphogenesis in Arabidopsis
Visit the PubMed ArticleTreatment of seedlings with 1 µm GR24 increased expression ... ninefold (DLK2 ... whereas 1 µm Z–CL increased expression less than twofold, and even 10 µm Z–CL was only half as active as 1 µm GR24
Scaffidi A, Waters MT, Ghisalberti EL, Dixon KW, Flematti GR, Smith SM - Carlactone-independent seedling morphogenesis in Arabidopsis
Visit the PubMed ArticleOur microarray dataset showed that several ABA- and drought-related genes are up-regulated in tic-2, including ... SNRK2.2
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed ArticleOur microarray dataset showed that several ABA- and drought-related genes are up-regulated in tic-2, including EARLY RESPONSIVE TO DEHYDRATIONs (ERD7 ... The elevated expression ... in tic-2 at dawn was additionally confirmed by qRT-PCR
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed ArticleCCA1 HIKING EXPEDITION 1 (CHE ... overexpressed in tic-2
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed ArticleEnhanced starch accumulation in tic-2 seems to be in contradiction with our transcriptome profile, as several key genes involved in starch degradation ... STARCH EXCESS 1 (SEX1 ... induced in tic-2 ... To confirm our microarray data, we determined transcript levels by a qRT-PCR analysis ... SEX1 ... consistently up-regulated in tic-2 relative to Col at dawn
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed Articlein tic-2 ... Induction of ... PHYA
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed ArticleCAT1 ... overexpressed in tic-2
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed ArticleIn our microarray data ... GSH1 (At4g23100 ... not found to be differentially expressed in tic-2, compared with the wild type
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed ArticleCAT3 ... arrhythmic in tic-2 under diurnal conditions, whereas they displayed diurnal expression pattern in Col
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed ArticleEnhanced starch accumulation in tic-2 seems to be in contradiction with our transcriptome profile, as several key genes involved in starch degradation ... AMYLASE 3 (AMY3 ... induced in tic-2 ... To confirm our microarray data, we determined transcript levels by a qRT-PCR analysis. AMY3 ... consistently up-regulated in tic-2 relative to Col at dawn
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed ArticleOur microarray dataset showed that several ABA- and drought-related genes are up-regulated in tic-2, including ... COLD REGULATED 15A (COR15a ... The elevated expression of COR15A ... in tic-2 at dawn was additionally confirmed by qRT-PCR
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed Articletic transcriptome profile ... arginine carboxylases ... At4g34710 ... which synthesize putrescine, were repressed in tic-2
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed Articletic transcriptome profile ... arginine carboxylases ... At2g16500 ... which synthesize putrescine, were repressed in tic-2
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed ArticlePRR3 ... overexpressed in tic-2
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed Articleour microarray data showed that ß-AMYLASE 3 (BAM3), which encodes a maltose-producing enzyme, is repressed in tic-2
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed ArticleOur microarray dataset showed that several ABA- and drought-related genes are up-regulated in tic-2, including ... RD19a
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed ArticleOur microarray dataset showed that several ABA- and drought-related genes are up-regulated in tic-2, including ... RESPONSE TO DESSICATION 22 (RD22 ... The elevated expression of ... RD22 in tic-2 at dawn was additionally confirmed by qRT-PCR
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed ArticleOur microarray dataset showed that several ABA- and drought-related genes are up-regulated in tic-2, including ... COR47
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed ArticleOur microarray dataset showed that several ABA- and drought-related genes are up-regulated in tic-2, including ... DEHYDRATATION RESPONSIVE ELEMENT BINDING PROTEIN 2A (DREB2A
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed Articletic-2 accumulated higher amounts of total glutathione (Figure 4a). Pools of both reduced (GSH) and oxidized (GSSG) glutathione were higher in tic-2; therefore, the GSH/GSSG ratio was similar to or slightly lower than in the wild type
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed Articleglutathione S-transferases ... GSTU8 ... reduced in tic-2
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed Articleperoxiredoxins including PER69 ... reduced in tic-2
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed ArticleEnhanced starch accumulation in tic-2 seems to be in contradiction with our transcriptome profile, as several key genes involved in starch degradation ... SEX4 ... induced in tic-2 ... To confirm our microarray data, we determined transcript levels by a qRT-PCR analysis ... SEX4 ... consistently up-regulated in tic-2 relative to Col at dawn
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed ArticleIn our microarray data ... GSH2 (At5g27380 ... not found to be differentially expressed in tic-2, compared with the wild type
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed Articleglutathione S-transferases (GST1 ... reduced in tic-2
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed Articleperoxiredoxins including ... PER1 ... reduced in tic-2
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed ArticleOur microarray dataset showed that several ABA- and drought-related genes are up-regulated in tic-2, including ... abscisic acid responsive element-binding factor (At1g49720 ... The elevated expression of ... At1g49720 ... in tic-2 at dawn was additionally confirmed by qRT-PCR
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed ArticleOur microarray dataset showed that several ABA- and drought-related genes are up-regulated in tic-2, including EARLY RESPONSIVE TO DEHYDRATION ... ERD3
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed ArticleTHYLAKOIDAL PEROXIDASE (tAPX ... overexpressed in tic-2
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed Articlewe observed that starvation-triggering genes ... At1g76410 ... induced in tic-2
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed Articlewe observed that starvation-triggering genes ... At3g59940 ... induced in tic-2
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed Articlein tic-2 ... misexpression of ... homeobox transcription factors ... HAT4
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed Articlein tic-2 ... misexpression of ... homeobox transcription factors (HAT2
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed ArticleCAT1 ... arrhythmic in tic-2 under diurnal conditions, whereas they displayed diurnal expression pattern in Col
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed ArticleCu-Zn SUPEROXIDE DISMUTASE (CSD2 ... reduced in tic-2
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed ArticleGST1 ... arrhythmic in tic-2 under diurnal conditions, whereas they displayed diurnal expression pattern in Col
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed ArticleOur microarray dataset showed that several ABA- and drought-related genes are up-regulated in tic-2, including EARLY RESPONSIVE TO DEHYDRATION ... ERD4
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed Articlein tic-2 ... Induction of PIF4
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed ArticleOur microarray dataset showed that several ABA- and drought-related genes are up-regulated in tic-2, including ... SNF1-RELATED PROTEIN KINASE 2.3 (SNRK2.3
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed ArticleOur microarray dataset showed that several ABA- and drought-related genes are up-regulated in tic-2, including ... ABA INSENSITIVE1 (ABI1
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed ArticleSENESCENCE-RELATED GENE 1 (SRG1 ... reduced in tic-2
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed ArticleEnhanced starch accumulation in tic-2 seems to be in contradiction with our transcriptome profile, as several key genes involved in starch degradation ... PHOSPHOGLUCAN WATER DIKINASE (PWD ... induced in tic-2 ... To confirm our microarray data, we determined transcript levels by a qRT-PCR analysis ... PWD ... consistently up-regulated in tic-2 relative to Col at dawn
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed Articlein tic-2 ... Induction of ... PIF6
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed ArticlePRR5 ... overexpressed in tic-2
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed Articleglutathione peroxidases (ATGPX7 ... reduced in tic-2
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed Articlein tic-2 ... a putative spermidine synthase (At5g19530) was slightly induced
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed ArticlePSEUDORESPONSE REGULATORS7 (PRR7 ... overexpressed in tic-2
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed ArticleCAT3 ... overexpressed in tic-2
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed ArticleLUX ARRHYTHMO/PCL1 (LUX ... overexpressed in tic-2
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed Articleperoxiredoxins including ... PER73 ... reduced in tic-2
Sanchez-Villarreal A, Shin J, Bujdoso N, Obata T, Neumann U, Du SX, Ding Z, Davis AM, Shindo T, Schmelzer E, Sulpice R, Nunes-Nesi A, Stitt M, Fernie AR, Davis SJ - TIME FOR COFFEE is an essential component in the maintenance of metabolic homeostasis in Arabidopsis thaliana
Visit the PubMed ArticleGFP fusion proteins of Arabidopsis ... WDR12 ... mainly localized to the nucleolus
Cho HK, Ahn CS, Lee HS, Kim JK, Pai HS - Pescadillo plays an essential role in plant cell growth and survival by modulating ribosome biogenesis
Visit the PubMed ArticleGFP fusion proteins of Arabidopsis BOP1 ... mainly localized to the nucleolus
Cho HK, Ahn CS, Lee HS, Kim JK, Pai HS - Pescadillo plays an essential role in plant cell growth and survival by modulating ribosome biogenesis
Visit the PubMed ArticleTo determine the subcellular localization of AtPES and its mutants, GFP fusion constructs of full-length AtPES and its mutants [carrying deletion of the PES-N domain (∆PES-N), deletion of the BRCT domain (∆BRCT), or a mutation of the potential sumoylation site, LKKE to LAAE (Sumo-m)] were expressed in N. benthamiana leaves via agroinfiltration. Confocal laser scanning microscopy of the leaf epidermal cells revealed that AtPES:GFP was predominantly localized in the nucleolus, although a faint signal was also detected in the nucleoplasm at longer exposure (Figure 2b). ∆PES-N:GFP, however, exhibited no nucleolar signal, but had diffuse nucleoplasmic distribution, suggesting the importance of the PES-N domain for nucleolar localization of PES in plant cells. Interestingly, ∆BRCT:GFP was strongly detected in the nucleolus and at discrete loci in the nucleoplasm
Cho HK, Ahn CS, Lee HS, Kim JK, Pai HS - Pescadillo plays an essential role in plant cell growth and survival by modulating ribosome biogenesis
Visit the PubMed ArticleEm6 ... was upregulated more strongly in response to ABA treatments in AtMBP-1 over-expressing plants
Kang M, Abdelmageed H, Lee S, Reichert A, Mysore KS, Allen RD - AtMBP-1, an alternative translation product of LOS2, affects abscisic acid responses and is modulated by the E3 ubiquitin ligase AtSAP5
Visit the PubMed Articlein AtMBP-1 over-expressing plants ... AFP ... 3 ... ABA responsiveness ... was not affected by AtMBP-1 expression
Kang M, Abdelmageed H, Lee S, Reichert A, Mysore KS, Allen RD - AtMBP-1, an alternative translation product of LOS2, affects abscisic acid responses and is modulated by the E3 ubiquitin ligase AtSAP5
Visit the PubMed ArticleVery little AtMBP-1 was observed to accumulate in 35S::AtMBP-1 transgenic plants, despite the fact that AtMBP-1 transcript levels were generally more abundant than those for LOS2 in 35S::LOS2 plants
Kang M, Abdelmageed H, Lee S, Reichert A, Mysore KS, Allen RD - AtMBP-1, an alternative translation product of LOS2, affects abscisic acid responses and is modulated by the E3 ubiquitin ligase AtSAP5
Visit the PubMed Articlethe response of ABF3 expression to ABA was not affected in 35S::AtMBP-1 plants
Kang M, Abdelmageed H, Lee S, Reichert A, Mysore KS, Allen RD - AtMBP-1, an alternative translation product of LOS2, affects abscisic acid responses and is modulated by the E3 ubiquitin ligase AtSAP5
Visit the PubMed Articlethe increase in STZ/ZAT10 expression at 4°C in 35S::AMBP-1 plants was delayed and partially suppressed, relative to WT plants (Figure S7), a finding that indicated that constitutive expression of AtMBP-1 down-regulates the responsiveness of STZ/ZAT10 to low temperature
Kang M, Abdelmageed H, Lee S, Reichert A, Mysore KS, Allen RD - AtMBP-1, an alternative translation product of LOS2, affects abscisic acid responses and is modulated by the E3 ubiquitin ligase AtSAP5
Visit the PubMed Articlein AtMBP-1 over-expressing plants ... AFP1 ... ABA responsiveness ... was not affected by AtMBP-1 expression
Kang M, Abdelmageed H, Lee S, Reichert A, Mysore KS, Allen RD - AtMBP-1, an alternative translation product of LOS2, affects abscisic acid responses and is modulated by the E3 ubiquitin ligase AtSAP5
Visit the PubMed ArticleRAB18 was upregulated more strongly in response to ABA treatments in AtMBP-1 over-expressing plants
Kang M, Abdelmageed H, Lee S, Reichert A, Mysore KS, Allen RD - AtMBP-1, an alternative translation product of LOS2, affects abscisic acid responses and is modulated by the E3 ubiquitin ligase AtSAP5
Visit the PubMed ArticleThe expression of ... ABI2 ... was not significantly affected in AtMBP-1 expressing plants compared with WT plants under ABA treatment
Kang M, Abdelmageed H, Lee S, Reichert A, Mysore KS, Allen RD - AtMBP-1, an alternative translation product of LOS2, affects abscisic acid responses and is modulated by the E3 ubiquitin ligase AtSAP5
Visit the PubMed ArticleAnalysis of fluorescence using confocal microscopy indicated that recombinant YFP-tagged protein in 35S::LOS2 expressing plants was localized in both cytosolic and nuclear cellular compartments
Kang M, Abdelmageed H, Lee S, Reichert A, Mysore KS, Allen RD - AtMBP-1, an alternative translation product of LOS2, affects abscisic acid responses and is modulated by the E3 ubiquitin ligase AtSAP5
Visit the PubMed ArticleThe expression of ABI1 ... was not significantly affected in AtMBP-1 expressing plants compared with WT plants under ABA treatment
Kang M, Abdelmageed H, Lee S, Reichert A, Mysore KS, Allen RD - AtMBP-1, an alternative translation product of LOS2, affects abscisic acid responses and is modulated by the E3 ubiquitin ligase AtSAP5
Visit the PubMed Articlein AtMBP-1 over-expressing plants ... SnRK2.3 ... ABA responsiveness ... was not affected by AtMBP-1 expression
Kang M, Abdelmageed H, Lee S, Reichert A, Mysore KS, Allen RD - AtMBP-1, an alternative translation product of LOS2, affects abscisic acid responses and is modulated by the E3 ubiquitin ligase AtSAP5
Visit the PubMed ArticleEm1 ... was upregulated more strongly in response to ABA treatments in AtMBP-1 over-expressing plants
Kang M, Abdelmageed H, Lee S, Reichert A, Mysore KS, Allen RD - AtMBP-1, an alternative translation product of LOS2, affects abscisic acid responses and is modulated by the E3 ubiquitin ligase AtSAP5
Visit the PubMed Articleexpression of ... ABI5 ... was more responsive to ABA in AtMBP-1 over-expressing plants
Kang M, Abdelmageed H, Lee S, Reichert A, Mysore KS, Allen RD - AtMBP-1, an alternative translation product of LOS2, affects abscisic acid responses and is modulated by the E3 ubiquitin ligase AtSAP5
Visit the PubMed ArticleAnalysis of fluorescence using confocal microscopy indicated that recombinant YFP-tagged protein in 35S::LOS2 expressing plants was localized in both cytosolic and nuclear cellular compartments, while the N-terminal truncated AtMBP-1–YFP fusion protein expressed in 35S::AtMBP-1 expressing plants was predominantly localized to nuclei
Kang M, Abdelmageed H, Lee S, Reichert A, Mysore KS, Allen RD - AtMBP-1, an alternative translation product of LOS2, affects abscisic acid responses and is modulated by the E3 ubiquitin ligase AtSAP5
Visit the PubMed Articlein AtMBP-1 over-expressing plants ... AFP ... 2 ... ABA responsiveness ... was not affected by AtMBP-1 expression
Kang M, Abdelmageed H, Lee S, Reichert A, Mysore KS, Allen RD - AtMBP-1, an alternative translation product of LOS2, affects abscisic acid responses and is modulated by the E3 ubiquitin ligase AtSAP5
Visit the PubMed Articlein AtMBP-1 over-expressing plants ... SnRK2.2 ... ABA responsiveness ... was not affected by AtMBP-1 expression
Kang M, Abdelmageed H, Lee S, Reichert A, Mysore KS, Allen RD - AtMBP-1, an alternative translation product of LOS2, affects abscisic acid responses and is modulated by the E3 ubiquitin ligase AtSAP5
Visit the PubMed Articleexpression of ... ABI4 ... was more responsive to ABA in AtMBP-1 over-expressing plants
Kang M, Abdelmageed H, Lee S, Reichert A, Mysore KS, Allen RD - AtMBP-1, an alternative translation product of LOS2, affects abscisic acid responses and is modulated by the E3 ubiquitin ligase AtSAP5
Visit the PubMed Articleexpression of ABI3 ... was more responsive to ABA in AtMBP-1 over-expressing plants
Kang M, Abdelmageed H, Lee S, Reichert A, Mysore KS, Allen RD - AtMBP-1, an alternative translation product of LOS2, affects abscisic acid responses and is modulated by the E3 ubiquitin ligase AtSAP5
Visit the PubMed ArticleTwenty-four hours after radical protrusion, no significant difference could be detected in the mRNA levels of these six genes between the wildtype and the elo3 samples, confirming that the cells were able to activate the transcription of these core cell cycle regulators ... when the small elo3 seedlings began to display signs of growth arrest ... three CYCs ... showed approximately 50% reduction in their mRNA levels in elo3 mutants
Skylar A, Matsuwaka S, Wu X - ELONGATA3 is required for shoot meristem cell cycle progression in Arabidopsis thaliana seedlings
Visit the PubMed ArticleTwenty-four hours after radical protrusion, no significant difference could be detected in the mRNA levels of these six genes between the wildtype and the elo3 samples, confirming that the cells were able to activate the transcription of these core cell cycle regulators ... when the small elo3 seedlings began to display signs of growth arrest ... no difference was found in CDKA;1 expression levels between the two samples
Skylar A, Matsuwaka S, Wu X - ELONGATA3 is required for shoot meristem cell cycle progression in Arabidopsis thaliana seedlings
Visit the PubMed Articlelevel of CYCB1;1 ... responded to sucrose stimulation in elo3 with ... increases ... To determine how soon the cell cycle regulators respond to the presence of exogenous sugar, we treated 7-day-old fully arrested small elo3-14 with 1.5% sucrose and compared the expression levels of the six core cell cycle genes before and after the treatment, using qRT-PCR. Compared to the 2 days it takes to have any visible phenotypic rescue in these seedlings, a moderate increase in the expression levels of all six genes could be detected after 4 h of treatment. Twenty-four hours of treatment resulted in four to nine fold of increase in their mRNA levels
Skylar A, Matsuwaka S, Wu X - ELONGATA3 is required for shoot meristem cell cycle progression in Arabidopsis thaliana seedlings
Visit the PubMed Articlein wild type, sucrose ... was able to significantly increase the level of CYCB1;1
Skylar A, Matsuwaka S, Wu X - ELONGATA3 is required for shoot meristem cell cycle progression in Arabidopsis thaliana seedlings
Visit the PubMed Articlelevel of ... CDKB1;1 ... responded to sucrose stimulation in elo3 with ... increases ... To determine how soon the cell cycle regulators respond to the presence of exogenous sugar, we treated 7-day-old fully arrested small elo3-14 with 1.5% sucrose and compared the expression levels of the six core cell cycle genes before and after the treatment, using qRT-PCR. Compared to the 2 days it takes to have any visible phenotypic rescue in these seedlings, a moderate increase in the expression levels of all six genes could be detected after 4 h of treatment. Twenty-four hours of treatment resulted in four to nine fold of increase in their mRNA levels
Skylar A, Matsuwaka S, Wu X - ELONGATA3 is required for shoot meristem cell cycle progression in Arabidopsis thaliana seedlings
Visit the PubMed Articlesugar-rescued elo3 seedlings ... clear increase in histone H4 expression ( Fig. 5H, compared to F), although it is still below the wildtype levels
Skylar A, Matsuwaka S, Wu X - ELONGATA3 is required for shoot meristem cell cycle progression in Arabidopsis thaliana seedlings
Visit the PubMed ArticleCDKA;1 ... responded to sucrose stimulation in elo3 with ... increases ... To determine how soon the cell cycle regulators respond to the presence of exogenous sugar, we treated 7-day-old fully arrested small elo3-14 with 1.5% sucrose and compared the expression levels of the six core cell cycle genes before and after the treatment, using qRT-PCR. Compared to the 2 days it takes to have any visible phenotypic rescue in these seedlings, a moderate increase in the expression levels of all six genes could be detected after 4 h of treatment. Twenty-four hours of treatment resulted in four to nine fold of increase in their mRNA levels
Skylar A, Matsuwaka S, Wu X - ELONGATA3 is required for shoot meristem cell cycle progression in Arabidopsis thaliana seedlings
Visit the PubMed Articlein wild type, sucrose ... was able to significantly increase the level of ... CDKB1;1
Skylar A, Matsuwaka S, Wu X - ELONGATA3 is required for shoot meristem cell cycle progression in Arabidopsis thaliana seedlings
Visit the PubMed ArticleTwenty-four hours after radical protrusion, no significant difference could be detected in the mRNA levels of these six genes between the wildtype and the elo3 samples, confirming that the cells were able to activate the transcription of these core cell cycle regulators ... when the small elo3 seedlings began to display signs of growth arrest ... three CYCs ... showed approximately 50% reduction in their mRNA levels in elo3 mutants
Skylar A, Matsuwaka S, Wu X - ELONGATA3 is required for shoot meristem cell cycle progression in Arabidopsis thaliana seedlings
Visit the PubMed Articlelevel of ... CDKB1;2 ... responded to sucrose stimulation in elo3 with ... increases ... To determine how soon the cell cycle regulators respond to the presence of exogenous sugar, we treated 7-day-old fully arrested small elo3-14 with 1.5% sucrose and compared the expression levels of the six core cell cycle genes before and after the treatment, using qRT-PCR. Compared to the 2 days it takes to have any visible phenotypic rescue in these seedlings, a moderate increase in the expression levels of all six genes could be detected after 4 h of treatment. Twenty-four hours of treatment resulted in four to nine fold of increase in their mRNA levels
Skylar A, Matsuwaka S, Wu X - ELONGATA3 is required for shoot meristem cell cycle progression in Arabidopsis thaliana seedlings
Visit the PubMed Articlein situ hybridization using an anti-sense probe of ELO3 ... In the shoot, it is found in the ... emerging leaves immediately after germination
Skylar A, Matsuwaka S, Wu X - ELONGATA3 is required for shoot meristem cell cycle progression in Arabidopsis thaliana seedlings
Visit the PubMed ArticleThree days after germination, STM is expressed in the wildtype shoot meristem and is cleared from the emerging leaf primordia ( Fig. 3B). At this stage, the STM domain encompassed the whole shoot meristem region in the small elo3-14 seedlings ( Fig. 3F), which is consistent with their failure in leaf initiation. Four days later, while the STM domain expanded in the wildtype shoot meristem ( Fig. 3D), it was no longer detectable in the growth arrested elo3 mutant seedlings
Skylar A, Matsuwaka S, Wu X - ELONGATA3 is required for shoot meristem cell cycle progression in Arabidopsis thaliana seedlings
Visit the PubMed ArticleCYCA2;1 ... responded to sucrose stimulation in elo3 with ... increases ... To determine how soon the cell cycle regulators respond to the presence of exogenous sugar, we treated 7-day-old fully arrested small elo3-14 with 1.5% sucrose and compared the expression levels of the six core cell cycle genes before and after the treatment, using qRT-PCR. Compared to the 2 days it takes to have any visible phenotypic rescue in these seedlings, a moderate increase in the expression levels of all six genes could be detected after 4 h of treatment. Twenty-four hours of treatment resulted in four to nine fold of increase in their mRNA levels
Skylar A, Matsuwaka S, Wu X - ELONGATA3 is required for shoot meristem cell cycle progression in Arabidopsis thaliana seedlings
Visit the PubMed ArticleTwenty-four hours after radical protrusion, no significant difference could be detected in the mRNA levels of these six genes between the wildtype and the elo3 samples, confirming that the cells were able to activate the transcription of these core cell cycle regulators ... when the small elo3 seedlings began to display signs of growth arrest ... CDKB1's ... showed approximately 50% reduction in their mRNA levels in elo3 mutants
Skylar A, Matsuwaka S, Wu X - ELONGATA3 is required for shoot meristem cell cycle progression in Arabidopsis thaliana seedlings
Visit the PubMed Articlein wild type, sucrose ... was able to significantly increase the level of ... CDKB1;2
Skylar A, Matsuwaka S, Wu X - ELONGATA3 is required for shoot meristem cell cycle progression in Arabidopsis thaliana seedlings
Visit the PubMed ArticleTwenty-four hours after radical protrusion, no significant difference could be detected in the mRNA levels of these six genes between the wildtype and the elo3 samples, confirming that the cells were able to activate the transcription of these core cell cycle regulators ... when the small elo3 seedlings began to display signs of growth arrest ... three CYCs ... showed approximately 50% reduction in their mRNA levels in elo3 mutants
Skylar A, Matsuwaka S, Wu X - ELONGATA3 is required for shoot meristem cell cycle progression in Arabidopsis thaliana seedlings
Visit the PubMed ArticleThe first step in seedling cell cycle activation is DNA replication (Barroco et al., 2005), and new histone molecules must be synthesized during this process. Therefore, the expression levels of the histone genes are a good indication of DNA synthesis activities. Two days after germination, in situ hybridization using an antisense probe against the histone H4 genes detected abundant signals in the shoot apex of the wildtype seedlings, both in the meristem and the developing leaf primordia ( Fig. 4A). In comparison, the majority of the elo3-14 seedlings showed very little to no histone H4 RNA signal in the shoot apex ( Fig. 4C). The remaining samples, which most likely represent the large class of elo3-14, had slightly more histone H4 expression, but remained significantly below the wildtype levels
Skylar A, Matsuwaka S, Wu X - ELONGATA3 is required for shoot meristem cell cycle progression in Arabidopsis thaliana seedlings
Visit the PubMed Articlein wild type, sucrose had little effect in enhancing the transcription of ... CYCA2;1
Skylar A, Matsuwaka S, Wu X - ELONGATA3 is required for shoot meristem cell cycle progression in Arabidopsis thaliana seedlings
Visit the PubMed ArticleCYCD3;1 ... responded to sucrose stimulation in elo3 with ... increases ... To determine how soon the cell cycle regulators respond to the presence of exogenous sugar, we treated 7-day-old fully arrested small elo3-14 with 1.5% sucrose and compared the expression levels of the six core cell cycle genes before and after the treatment, using qRT-PCR. Compared to the 2 days it takes to have any visible phenotypic rescue in these seedlings, a moderate increase in the expression levels of all six genes could be detected after 4 h of treatment. Twenty-four hours of treatment resulted in four to nine fold of increase in their mRNA levels
Skylar A, Matsuwaka S, Wu X - ELONGATA3 is required for shoot meristem cell cycle progression in Arabidopsis thaliana seedlings
Visit the PubMed Articlein situ hybridization using an anti-sense probe of ELO3 detected ELO3 mRNA ... In the shoot, it is found in the meristem ... immediately after germination
Skylar A, Matsuwaka S, Wu X - ELONGATA3 is required for shoot meristem cell cycle progression in Arabidopsis thaliana seedlings
Visit the PubMed Articlein situ hybridization using an anti-sense probe of ELO3 detected ELO3 mRNA in the meristematic regions of both the shoot and the root
Skylar A, Matsuwaka S, Wu X - ELONGATA3 is required for shoot meristem cell cycle progression in Arabidopsis thaliana seedlings
Visit the PubMed Articlein wild type, sucrose had little effect in enhancing the transcription of ... CYCD3;1
Skylar A, Matsuwaka S, Wu X - ELONGATA3 is required for shoot meristem cell cycle progression in Arabidopsis thaliana seedlings
Visit the PubMed Articlein wild type, sucrose had little effect in enhancing the transcription of CDKA;1
Skylar A, Matsuwaka S, Wu X - ELONGATA3 is required for shoot meristem cell cycle progression in Arabidopsis thaliana seedlings
Visit the PubMed Articlesitu hybridization using an anti-sense probe of ELO3 ... this expression persists and expands into young leaves as the seedlings develop
Skylar A, Matsuwaka S, Wu X - ELONGATA3 is required for shoot meristem cell cycle progression in Arabidopsis thaliana seedlings
Visit the PubMed Articlethe stem cell cluster in the rescued elo3 seedlings remains significantly smaller than in the wildtype samples, as measured by the activities of the CLV3::GUS reporter
Skylar A, Matsuwaka S, Wu X - ELONGATA3 is required for shoot meristem cell cycle progression in Arabidopsis thaliana seedlings
Visit the PubMed ArticleTwenty-four hours after radical protrusion, no significant difference could be detected in the mRNA levels of these six genes between the wildtype and the elo3 samples, confirming that the cells were able to activate the transcription of these core cell cycle regulators ... when the small elo3 seedlings began to display signs of growth arrest ... CDKB1's ... showed approximately 50% reduction in their mRNA levels in elo3 mutants
Skylar A, Matsuwaka S, Wu X - ELONGATA3 is required for shoot meristem cell cycle progression in Arabidopsis thaliana seedlings
Visit the PubMed Articleno difference could be seen in CLV3 expression between the wildtype ( Fig. 3A) and elo3 ( Fig. 3E) seedlings, as detected by the activity of the CLV3::GUS reporter ( Brand et al., 2002). One week later, as the wildtype shoot meristem grew into a dome-shaped structure, CLV3::GUS activities increased significantly ( Fig. 3C). In comparison, the CLV3::GUS domain was present but much smaller in elo3-14 of the same age ( Fig. 3G, compared to C). Surprisingly, we were able to detect CLV3::GUS activity in the mutant shoot meristem at least 10 days after germination, indicating that the shoot stem cell population in the growth-arrested elo3 mutants remain active
Skylar A, Matsuwaka S, Wu X - ELONGATA3 is required for shoot meristem cell cycle progression in Arabidopsis thaliana seedlings
Visit the PubMed Articlesex1-1 ... reduced KS expression the next day
Paparelli E, Parlanti S, Gonzali S, Novi G, Mariotti L, Ceccarelli N, van Dongen JT, Kölling K, Zeeman SC, Perata P - Nighttime sugar starvation orchestrates gibberellin biosynthesis and plant growth in Arabidopsis
Visit the PubMed Articlewe kept a batch of wild-type plants under normal day/night conditions (Figure 6A), while a second batch of plants was exposed to low light for 1 d, followed by two additional days under normal day/night conditions ... EXP1, a GA-induced expansin (see Supplemental Figure 13B online) expressed in stomatal guard cells (Zhang et al., 2011), whose expression rose rapidly each night (Figure 6A). In plants that were exposed to low light for just 1 d, EXP1 expression was severely dampened the night immediately following the period when there had been no KS expression peak
Paparelli E, Parlanti S, Gonzali S, Novi G, Mariotti L, Ceccarelli N, van Dongen JT, Kölling K, Zeeman SC, Perata P - Nighttime sugar starvation orchestrates gibberellin biosynthesis and plant growth in Arabidopsis
Visit the PubMed ArticleSo, was the altered KS expression pattern unique to starch mutants, or might it also be observed in wild-type plants experiencing night starvation? To examine this, we kept a batch of wild-type plants under normal day/night conditions (Figure 6A), while a second batch of plants was exposed to low light for 1 d, followed by two additional days under normal day/night conditions (Figure 6B). The low light treatment resulted in low starch content at the end of the first day, together with high expression of the sugar starvation marker (Figure 6B; see Supplemental Figures 12A and 12B online). Over the next 2 d, starch synthesis resumed and the expression of the marker for sugar starvation was consequently low (Figure 6B). In line with what we observed in starch mutants, while KS expression was unaffected during the day under low light, the afternoon peak of KS expression was lost during the second day but resumed the third day
Paparelli E, Parlanti S, Gonzali S, Novi G, Mariotti L, Ceccarelli N, van Dongen JT, Kölling K, Zeeman SC, Perata P - Nighttime sugar starvation orchestrates gibberellin biosynthesis and plant growth in Arabidopsis
Visit the PubMed ArticleThe starchless mutants pgm ... showed expression of the typical marker for sugar starvation (dark inducible6 [DIN6]; Gonzali et al., 2006; Usadel et al., 2008) during the night
Paparelli E, Parlanti S, Gonzali S, Novi G, Mariotti L, Ceccarelli N, van Dongen JT, Kölling K, Zeeman SC, Perata P - Nighttime sugar starvation orchestrates gibberellin biosynthesis and plant growth in Arabidopsis
Visit the PubMed ArticleIn order to gain direct evidence linking night starvation to the regulation of KS, we sprayed Suc on the leaves of ... sex1-1 during the night. Interestingly, the Suc treatment restored the peak of KS expression the following day
Paparelli E, Parlanti S, Gonzali S, Novi G, Mariotti L, Ceccarelli N, van Dongen JT, Kölling K, Zeeman SC, Perata P - Nighttime sugar starvation orchestrates gibberellin biosynthesis and plant growth in Arabidopsis
Visit the PubMed ArticleReductions in the expression of copalyl diphosphate synthase ... were observed in pgm
Paparelli E, Parlanti S, Gonzali S, Novi G, Mariotti L, Ceccarelli N, van Dongen JT, Kölling K, Zeeman SC, Perata P - Nighttime sugar starvation orchestrates gibberellin biosynthesis and plant growth in Arabidopsis
Visit the PubMed Articlein pgm ... the diurnal expression of GA20ox1 (At4g25420), which is under circadian control (Hisamatsu et al., 2005), was unaffected
Paparelli E, Parlanti S, Gonzali S, Novi G, Mariotti L, Ceccarelli N, van Dongen JT, Kölling K, Zeeman SC, Perata P - Nighttime sugar starvation orchestrates gibberellin biosynthesis and plant growth in Arabidopsis
Visit the PubMed ArticleIn order to gain direct evidence linking night starvation to the regulation of KS, we sprayed Suc on the leaves of pgm ... during the night. Interestingly, the Suc treatment restored the peak of KS expression the following day
Paparelli E, Parlanti S, Gonzali S, Novi G, Mariotti L, Ceccarelli N, van Dongen JT, Kölling K, Zeeman SC, Perata P - Nighttime sugar starvation orchestrates gibberellin biosynthesis and plant growth in Arabidopsis
Visit the PubMed ArticleThe starchless mutant ... adg1-1 ... showed expression of the typical marker for sugar starvation (dark inducible6 [DIN6]; Gonzali et al., 2006; Usadel et al., 2008) during the night
Paparelli E, Parlanti S, Gonzali S, Novi G, Mariotti L, Ceccarelli N, van Dongen JT, Kölling K, Zeeman SC, Perata P - Nighttime sugar starvation orchestrates gibberellin biosynthesis and plant growth in Arabidopsis
Visit the PubMed ArticleReductions in the expression of ... ent-kaurene oxidase were observed in pgm
Paparelli E, Parlanti S, Gonzali S, Novi G, Mariotti L, Ceccarelli N, van Dongen JT, Kölling K, Zeeman SC, Perata P - Nighttime sugar starvation orchestrates gibberellin biosynthesis and plant growth in Arabidopsis
Visit the PubMed Articlesex4-3 ... induction of the sugar starvation marker at night ... DIN6
Paparelli E, Parlanti S, Gonzali S, Novi G, Mariotti L, Ceccarelli N, van Dongen JT, Kölling K, Zeeman SC, Perata P - Nighttime sugar starvation orchestrates gibberellin biosynthesis and plant growth in Arabidopsis
Visit the PubMed Articleadg1-1 ... Sugar starvation at night was confirmed by other starvation-induced markers, such as ... trehalose 6-P synthase
Paparelli E, Parlanti S, Gonzali S, Novi G, Mariotti L, Ceccarelli N, van Dongen JT, Kölling K, Zeeman SC, Perata P - Nighttime sugar starvation orchestrates gibberellin biosynthesis and plant growth in Arabidopsis
Visit the PubMed Articlewe kept a batch of wild-type plants under normal day/night conditions (Figure 6A), while a second batch of plants was exposed to low light for 1 d, followed by two additional days under normal day/night conditions (Figure 6B ... The expression pattern of GA20ox, on the other hand, was unaffected by the low light over the 3 d
Paparelli E, Parlanti S, Gonzali S, Novi G, Mariotti L, Ceccarelli N, van Dongen JT, Kölling K, Zeeman SC, Perata P - Nighttime sugar starvation orchestrates gibberellin biosynthesis and plant growth in Arabidopsis
Visit the PubMed Articlesex1-1 ... induction of the sugar starvation marker at night ... DIN6
Paparelli E, Parlanti S, Gonzali S, Novi G, Mariotti L, Ceccarelli N, van Dongen JT, Kölling K, Zeeman SC, Perata P - Nighttime sugar starvation orchestrates gibberellin biosynthesis and plant growth in Arabidopsis
Visit the PubMed Articleadg1-1 ... Sugar starvation at night was confirmed by other starvation-induced markers, such as At1g76410
Paparelli E, Parlanti S, Gonzali S, Novi G, Mariotti L, Ceccarelli N, van Dongen JT, Kölling K, Zeeman SC, Perata P - Nighttime sugar starvation orchestrates gibberellin biosynthesis and plant growth in Arabidopsis
Visit the PubMed Articlewe kept a batch of wild-type plants under normal day/night conditions (Figure 6A), while a second batch of plants was exposed to low light for 1 d, followed by two additional days under normal day/night conditions ... Although EXP10 was not expressed when the KS expression peak was abolished following a night starvation event (Figure 6B), interestingly it was restored the next day
Paparelli E, Parlanti S, Gonzali S, Novi G, Mariotti L, Ceccarelli N, van Dongen JT, Kölling K, Zeeman SC, Perata P - Nighttime sugar starvation orchestrates gibberellin biosynthesis and plant growth in Arabidopsis
Visit the PubMed ArticleThe increased expression of KS in the afternoon correlates well with higher levels of ent-kaurene in the wild type at the end of the day
Paparelli E, Parlanti S, Gonzali S, Novi G, Mariotti L, Ceccarelli N, van Dongen JT, Kölling K, Zeeman SC, Perata P - Nighttime sugar starvation orchestrates gibberellin biosynthesis and plant growth in Arabidopsis
Visit the PubMed ArticleWhen grown at twice the normal light intensity ... in ... sex1-1 ... afternoon peak of KS expression was present
Paparelli E, Parlanti S, Gonzali S, Novi G, Mariotti L, Ceccarelli N, van Dongen JT, Kölling K, Zeeman SC, Perata P - Nighttime sugar starvation orchestrates gibberellin biosynthesis and plant growth in Arabidopsis
Visit the PubMed ArticleThe expression of ent-kaurene synthase (KS) during the diurnal cycle had a distinct pattern, which peaked in the afternoon. Strikingly, this peak was absent in ... pgm
Paparelli E, Parlanti S, Gonzali S, Novi G, Mariotti L, Ceccarelli N, van Dongen JT, Kölling K, Zeeman SC, Perata P - Nighttime sugar starvation orchestrates gibberellin biosynthesis and plant growth in Arabidopsis
Visit the PubMed Articlesex4-3 ... reduced KS expression the next day
Paparelli E, Parlanti S, Gonzali S, Novi G, Mariotti L, Ceccarelli N, van Dongen JT, Kölling K, Zeeman SC, Perata P - Nighttime sugar starvation orchestrates gibberellin biosynthesis and plant growth in Arabidopsis
Visit the PubMed Articlein ... sex1-1 ... GA3ox1 (At1g15550) expression was also largely unaffected
Paparelli E, Parlanti S, Gonzali S, Novi G, Mariotti L, Ceccarelli N, van Dongen JT, Kölling K, Zeeman SC, Perata P - Nighttime sugar starvation orchestrates gibberellin biosynthesis and plant growth in Arabidopsis
Visit the PubMed Articlepgm ... showed ... negligible expression of KS the following day
Paparelli E, Parlanti S, Gonzali S, Novi G, Mariotti L, Ceccarelli N, van Dongen JT, Kölling K, Zeeman SC, Perata P - Nighttime sugar starvation orchestrates gibberellin biosynthesis and plant growth in Arabidopsis
Visit the PubMed ArticleReductions in the expression of copalyl diphosphate synthase ... were observed in ... sex1-1
Paparelli E, Parlanti S, Gonzali S, Novi G, Mariotti L, Ceccarelli N, van Dongen JT, Kölling K, Zeeman SC, Perata P - Nighttime sugar starvation orchestrates gibberellin biosynthesis and plant growth in Arabidopsis
Visit the PubMed Articlepgm ... Sugar starvation at night was confirmed by other starvation-induced markers, such as At1g76410
Paparelli E, Parlanti S, Gonzali S, Novi G, Mariotti L, Ceccarelli N, van Dongen JT, Kölling K, Zeeman SC, Perata P - Nighttime sugar starvation orchestrates gibberellin biosynthesis and plant growth in Arabidopsis
Visit the PubMed Articlein pgm ... GA3ox1 (At1g15550) expression was also largely unaffected
Paparelli E, Parlanti S, Gonzali S, Novi G, Mariotti L, Ceccarelli N, van Dongen JT, Kölling K, Zeeman SC, Perata P - Nighttime sugar starvation orchestrates gibberellin biosynthesis and plant growth in Arabidopsis
Visit the PubMed ArticleThe expression of ent-kaurene synthase (KS) during the diurnal cycle had a distinct pattern, which peaked in the afternoon. Strikingly, this peak was absent in ... sex1-1
Paparelli E, Parlanti S, Gonzali S, Novi G, Mariotti L, Ceccarelli N, van Dongen JT, Kölling K, Zeeman SC, Perata P - Nighttime sugar starvation orchestrates gibberellin biosynthesis and plant growth in Arabidopsis
Visit the PubMed ArticleSuc treatment restored the peak of KS expression the following day ... using the adg1-1 mutant
Paparelli E, Parlanti S, Gonzali S, Novi G, Mariotti L, Ceccarelli N, van Dongen JT, Kölling K, Zeeman SC, Perata P - Nighttime sugar starvation orchestrates gibberellin biosynthesis and plant growth in Arabidopsis
Visit the PubMed Articleadg1-1 ... showed ... negligible expression of KS the following day
Paparelli E, Parlanti S, Gonzali S, Novi G, Mariotti L, Ceccarelli N, van Dongen JT, Kölling K, Zeeman SC, Perata P - Nighttime sugar starvation orchestrates gibberellin biosynthesis and plant growth in Arabidopsis
Visit the PubMed ArticleEXP10 ... was upregulated by GAs (see Supplemental Figure 13A online) and displayed a diurnal expression peak at the end of the day
Paparelli E, Parlanti S, Gonzali S, Novi G, Mariotti L, Ceccarelli N, van Dongen JT, Kölling K, Zeeman SC, Perata P - Nighttime sugar starvation orchestrates gibberellin biosynthesis and plant growth in Arabidopsis
Visit the PubMed Articlepgm ... Sugar starvation at night was confirmed by other starvation-induced markers, such as ... trehalose 6-P synthase
Paparelli E, Parlanti S, Gonzali S, Novi G, Mariotti L, Ceccarelli N, van Dongen JT, Kölling K, Zeeman SC, Perata P - Nighttime sugar starvation orchestrates gibberellin biosynthesis and plant growth in Arabidopsis
Visit the PubMed Articlein ... sex1-1 ... the diurnal expression of GA20ox1 (At4g25420), which is under circadian control (Hisamatsu et al., 2005), was unaffected
Paparelli E, Parlanti S, Gonzali S, Novi G, Mariotti L, Ceccarelli N, van Dongen JT, Kölling K, Zeeman SC, Perata P - Nighttime sugar starvation orchestrates gibberellin biosynthesis and plant growth in Arabidopsis
Visit the PubMed ArticleWhen grown at twice the normal light intensity ... in pgm ... afternoon peak of KS expression was present
Paparelli E, Parlanti S, Gonzali S, Novi G, Mariotti L, Ceccarelli N, van Dongen JT, Kölling K, Zeeman SC, Perata P - Nighttime sugar starvation orchestrates gibberellin biosynthesis and plant growth in Arabidopsis
Visit the PubMed ArticleIn lsf1-1, which has the least severe starch-excess phenotype of the mutants studied (Comparot-Moss et al., 2010), KS expression was the closest to the wild-type expression
Paparelli E, Parlanti S, Gonzali S, Novi G, Mariotti L, Ceccarelli N, van Dongen JT, Kölling K, Zeeman SC, Perata P - Nighttime sugar starvation orchestrates gibberellin biosynthesis and plant growth in Arabidopsis
Visit the PubMed ArticleABCG9pro:GUS ... visualized in the vascular system of the cotyledons
Le Hir R, Sorin C, Chakraborti D, Moritz T, Schaller H, Tellier F, Robert S, Morin H, Bako L, Bellini C - ABCG9, ABCG11 and ABCG14 ABC transporters are required for vascular development in Arabidopsis
Visit the PubMed ArticleIn 4-week-old rosette leaves ... ABCG11pro:GUS ... expressed in the vascular system
Le Hir R, Sorin C, Chakraborti D, Moritz T, Schaller H, Tellier F, Robert S, Morin H, Bako L, Bellini C - ABCG9, ABCG11 and ABCG14 ABC transporters are required for vascular development in Arabidopsis
Visit the PubMed ArticleTranscripts for ... ABCG14 were found in all organs analyzed, with an overall higher relative transcript amount in the aerial parts compared with the root
Le Hir R, Sorin C, Chakraborti D, Moritz T, Schaller H, Tellier F, Robert S, Morin H, Bako L, Bellini C - ABCG9, ABCG11 and ABCG14 ABC transporters are required for vascular development in Arabidopsis
Visit the PubMed ArticleIn 4-week-old rosette leaves ... ABCG14pro:GUS ... expressed in the vascular system
Le Hir R, Sorin C, Chakraborti D, Moritz T, Schaller H, Tellier F, Robert S, Morin H, Bako L, Bellini C - ABCG9, ABCG11 and ABCG14 ABC transporters are required for vascular development in Arabidopsis
Visit the PubMed ArticleTranscripts for ABCG9 ... were found in all organs analyzed, with an overall higher relative transcript amount in the aerial parts compared with the root
Le Hir R, Sorin C, Chakraborti D, Moritz T, Schaller H, Tellier F, Robert S, Morin H, Bako L, Bellini C - ABCG9, ABCG11 and ABCG14 ABC transporters are required for vascular development in Arabidopsis
Visit the PubMed Articleexpression of ABCG11pro:GUS was identified in the rosette leaf epidermis
Le Hir R, Sorin C, Chakraborti D, Moritz T, Schaller H, Tellier F, Robert S, Morin H, Bako L, Bellini C - ABCG9, ABCG11 and ABCG14 ABC transporters are required for vascular development in Arabidopsis
Visit the PubMed ArticleWe first investigated the subcellular localization of ABCG9 ... after transfection of Arabidopsis mesophyll protoplasts prepared from transgenic seedlings expressing the plasma membrane marker low temperature induced protein 6b (GFP–LTi6b) (Cutler et al., 2000) with constructs encoding transcriptional fusions with red fluorescent protein (RFP ... the GFP fluorescent signal (Figure 5a,e) co-localized with the RFP fluorescent signal ... at the plasma membrane of transformed cells ... ABCG9 ... localize to the plasma membrane
Le Hir R, Sorin C, Chakraborti D, Moritz T, Schaller H, Tellier F, Robert S, Morin H, Bako L, Bellini C - ABCG9, ABCG11 and ABCG14 ABC transporters are required for vascular development in Arabidopsis
Visit the PubMed ArticleWe first investigated the subcellular localization of ... ABCG14 after transfection of Arabidopsis mesophyll protoplasts prepared from transgenic seedlings expressing the plasma membrane marker low temperature induced protein 6b (GFP–LTi6b) (Cutler et al., 2000) with constructs encoding transcriptional fusions with red fluorescent protein (RFP ... the GFP fluorescent signal (Figure 5a,e) co-localized with the RFP fluorescent signal ... at the plasma membrane of transformed cells ... indicating that ... ABCG14 localize to the plasma membrane
Le Hir R, Sorin C, Chakraborti D, Moritz T, Schaller H, Tellier F, Robert S, Morin H, Bako L, Bellini C - ABCG9, ABCG11 and ABCG14 ABC transporters are required for vascular development in Arabidopsis
Visit the PubMed ArticleTranscripts for ... ABCG11 ... were found in all organs analyzed, with an overall higher relative transcript amount in the aerial parts compared with the root
Le Hir R, Sorin C, Chakraborti D, Moritz T, Schaller H, Tellier F, Robert S, Morin H, Bako L, Bellini C - ABCG9, ABCG11 and ABCG14 ABC transporters are required for vascular development in Arabidopsis
Visit the PubMed ArticleIn 4-week-old rosette leaves, ABCG9pro:GUS ... expressed in the vascular system
Le Hir R, Sorin C, Chakraborti D, Moritz T, Schaller H, Tellier F, Robert S, Morin H, Bako L, Bellini C - ABCG9, ABCG11 and ABCG14 ABC transporters are required for vascular development in Arabidopsis
Visit the PubMed Articleaddition, immunolocalization of a HA-tagged version of ... ABCG14 in ... complemented lines confirmed that ... proteins are localized to the plasma membrane in planta
Le Hir R, Sorin C, Chakraborti D, Moritz T, Schaller H, Tellier F, Robert S, Morin H, Bako L, Bellini C - ABCG9, ABCG11 and ABCG14 ABC transporters are required for vascular development in Arabidopsis
Visit the PubMed ArticleThe ABCG9pro:GUS signal was restricted to the petiole main vein
Le Hir R, Sorin C, Chakraborti D, Moritz T, Schaller H, Tellier F, Robert S, Morin H, Bako L, Bellini C - ABCG9, ABCG11 and ABCG14 ABC transporters are required for vascular development in Arabidopsis
Visit the PubMed ArticleABCG11pro:GUS ... visualized in the vascular system of the cotyledons
Le Hir R, Sorin C, Chakraborti D, Moritz T, Schaller H, Tellier F, Robert S, Morin H, Bako L, Bellini C - ABCG9, ABCG11 and ABCG14 ABC transporters are required for vascular development in Arabidopsis
Visit the PubMed Articleimmunolocalization of a HA-tagged version of ABCG9 ... in ... complemented lines confirmed that ... proteins are localized to the plasma membrane in planta
Le Hir R, Sorin C, Chakraborti D, Moritz T, Schaller H, Tellier F, Robert S, Morin H, Bako L, Bellini C - ABCG9, ABCG11 and ABCG14 ABC transporters are required for vascular development in Arabidopsis
Visit the PubMed ArticleABCG14pro:GUS ... visualized in the vascular system of the cotyledons
Le Hir R, Sorin C, Chakraborti D, Moritz T, Schaller H, Tellier F, Robert S, Morin H, Bako L, Bellini C - ABCG9, ABCG11 and ABCG14 ABC transporters are required for vascular development in Arabidopsis
Visit the PubMed Articleinducible suppression of RBR ... Analysis of transcripts for the control genes ... AtTMM (TOO MANY MOUTHS) indicated an increase in ... mean level in line with previous reports (Borghi et al., 2010), although these differences were not significantly different under our conditions
Dorca-Fornell C, Pajor R, Lehmeier C, Pérez-Bueno M, Bauch M, Sloan J, Osborne C, Rolfe S, Sturrock C, Mooney S, Fleming A - Increased leaf mesophyll porosity following transient retinoblastoma-related protein silencing is revealed by microcomputed tomography imaging and leads to a system-level physiological response to the altered cell division pattern
Visit the PubMed ArticlePSBQ–L ... extrinsic sub-units of photosystem II) showed a decrease in the transcript level 24 h after suppression of RBR
Dorca-Fornell C, Pajor R, Lehmeier C, Pérez-Bueno M, Bauch M, Sloan J, Osborne C, Rolfe S, Sturrock C, Mooney S, Fleming A - Increased leaf mesophyll porosity following transient retinoblastoma-related protein silencing is revealed by microcomputed tomography imaging and leads to a system-level physiological response to the altered cell division pattern
Visit the PubMed Articleinducible suppression of RBR was followed by a decrease in the transcript level for AtAGP18 (as previously reported)
Dorca-Fornell C, Pajor R, Lehmeier C, Pérez-Bueno M, Bauch M, Sloan J, Osborne C, Rolfe S, Sturrock C, Mooney S, Fleming A - Increased leaf mesophyll porosity following transient retinoblastoma-related protein silencing is revealed by microcomputed tomography imaging and leads to a system-level physiological response to the altered cell division pattern
Visit the PubMed Articleinducible suppression of RBR ... Analysis of transcripts for the control genes AtSPCH (SPEECHLESS ... indicated an increase in ... mean level in line with previous reports (Borghi et al., 2010), although these differences were not significantly different under our conditions
Dorca-Fornell C, Pajor R, Lehmeier C, Pérez-Bueno M, Bauch M, Sloan J, Osborne C, Rolfe S, Sturrock C, Mooney S, Fleming A - Increased leaf mesophyll porosity following transient retinoblastoma-related protein silencing is revealed by microcomputed tomography imaging and leads to a system-level physiological response to the altered cell division pattern
Visit the PubMed Articleinducible suppression of RBR was followed by a decrease in the transcript level ... for AtAGP19
Dorca-Fornell C, Pajor R, Lehmeier C, Pérez-Bueno M, Bauch M, Sloan J, Osborne C, Rolfe S, Sturrock C, Mooney S, Fleming A - Increased leaf mesophyll porosity following transient retinoblastoma-related protein silencing is revealed by microcomputed tomography imaging and leads to a system-level physiological response to the altered cell division pattern
Visit the PubMed ArticlePSBO ... extrinsic sub-units of photosystem II) showed a decrease in the transcript level 24 h after suppression of RBR
Dorca-Fornell C, Pajor R, Lehmeier C, Pérez-Bueno M, Bauch M, Sloan J, Osborne C, Rolfe S, Sturrock C, Mooney S, Fleming A - Increased leaf mesophyll porosity following transient retinoblastoma-related protein silencing is revealed by microcomputed tomography imaging and leads to a system-level physiological response to the altered cell division pattern
Visit the PubMed ArticleTen genes involved in the flavonoid biosynthetic pathway were up-regulated 3.27- to 24.35-fold in mTCP3 plants (Table 1). These include the regulatory R2R3-MYB gene PAP1
Li S, Zachgo S - TCP3 interacts with R2R3-MYB proteins, promotes flavonoid biosynthesis and negatively regulates the auxin response in Arabidopsis thaliana
Visit the PubMed Articlein mTCP3 plants ... Genes that negatively affect the auxin response, such as CYP83B1/SUR2, which encodes a negative regulator of auxin production ... were up-regulated 3.27 ... fold
Li S, Zachgo S - TCP3 interacts with R2R3-MYB proteins, promotes flavonoid biosynthesis and negatively regulates the auxin response in Arabidopsis thaliana
Visit the PubMed ArticleA 2.07- to 8.62-fold down-regulation was observed for auxin signaling-related genes in mTCP3 plants. These genes ... encode the indole-3-acetic acid amido synthase (GH3.17
Li S, Zachgo S - TCP3 interacts with R2R3-MYB proteins, promotes flavonoid biosynthesis and negatively regulates the auxin response in Arabidopsis thaliana
Visit the PubMed ArticleA 2.07- to 8.62-fold down-regulation was observed for auxin signaling-related genes in mTCP3 plants. These genes encode ... the auxin-inducible transcription factor ... AUX/IAA29
Li S, Zachgo S - TCP3 interacts with R2R3-MYB proteins, promotes flavonoid biosynthesis and negatively regulates the auxin response in Arabidopsis thaliana
Visit the PubMed ArticleTen genes involved in the flavonoid biosynthetic pathway were up-regulated 3.27- to 24.35-fold in mTCP3 plants (Table 1). These include ... structural genes encoding key flavonoid biosynthetic enzymes, such as ... CHS/TT4
Li S, Zachgo S - TCP3 interacts with R2R3-MYB proteins, promotes flavonoid biosynthesis and negatively regulates the auxin response in Arabidopsis thaliana
Visit the PubMed ArticleEnhanced expression was observed for auxin-related genes in TCP3SRDX plants, such as ... PAR1 (3.14-fold), that encodes a transcriptional repressor of the indole-3-acetic acid-responsive genes
Li S, Zachgo S - TCP3 interacts with R2R3-MYB proteins, promotes flavonoid biosynthesis and negatively regulates the auxin response in Arabidopsis thaliana
Visit the PubMed ArticleA 2.07- to 8.62-fold down-regulation was observed for auxin signaling-related genes in mTCP3 plants. These genes encode proteins that participate in polar auxin transport ... AUX1
Li S, Zachgo S - TCP3 interacts with R2R3-MYB proteins, promotes flavonoid biosynthesis and negatively regulates the auxin response in Arabidopsis thaliana
Visit the PubMed ArticleA 2.07- to 8.62-fold down-regulation was observed for auxin signaling-related genes in mTCP3 plants. These genes encode proteins that participate in polar auxin transport ... ATP-binding cassette transporter 1/PGP1
Li S, Zachgo S - TCP3 interacts with R2R3-MYB proteins, promotes flavonoid biosynthesis and negatively regulates the auxin response in Arabidopsis thaliana
Visit the PubMed ArticleA 2.07- to 8.62-fold down-regulation was observed for auxin signaling-related genes in mTCP3 plants. These genes ... encode ... the auxin receptor ... TIR1
Li S, Zachgo S - TCP3 interacts with R2R3-MYB proteins, promotes flavonoid biosynthesis and negatively regulates the auxin response in Arabidopsis thaliana
Visit the PubMed ArticleTen genes involved in the flavonoid biosynthetic pathway were up-regulated 3.27- to 24.35-fold in mTCP3 plants (Table 1). These include ... structural genes encoding key flavonoid biosynthetic enzymes, such as ... DFR/TT3
Li S, Zachgo S - TCP3 interacts with R2R3-MYB proteins, promotes flavonoid biosynthesis and negatively regulates the auxin response in Arabidopsis thaliana
Visit the PubMed ArticleRelative to wild-type plants (Figure 3a,d), expression of mTCP3 induced a theeefold reduction in the pDR5::GUS reporter activity
Li S, Zachgo S - TCP3 interacts with R2R3-MYB proteins, promotes flavonoid biosynthesis and negatively regulates the auxin response in Arabidopsis thaliana
Visit the PubMed ArticleEnhanced expression was observed for auxin-related genes in TCP3SRDX plants, such as AUX/IAA29 (3.24-fold
Li S, Zachgo S - TCP3 interacts with R2R3-MYB proteins, promotes flavonoid biosynthesis and negatively regulates the auxin response in Arabidopsis thaliana
Visit the PubMed ArticleA 2.07- to 8.62-fold down-regulation was observed for auxin signaling-related genes in mTCP3 plants. These genes encode proteins that participate in polar auxin transport ... ATP-binding cassette transporter 19/PGP19
Li S, Zachgo S - TCP3 interacts with R2R3-MYB proteins, promotes flavonoid biosynthesis and negatively regulates the auxin response in Arabidopsis thaliana
Visit the PubMed ArticleTo investigate whether addition of exogenous auxin is able to rescue the reduced auxin response observed in mTCP3 plants, wild-type, mTCP3 and TCP3SRDX plantlets expressing pDR5::GUS were exposed for 15 h prior to GUS staining to 1-naphthaleneacetic acid (NAA), which is more long-lived than indole-3-acetic acid (IAA). Incubation of mTCP3 seedlings in the presence of NAA did not result in increased activity of the pDR5::GUS reporter (Figure 3d,g,h), in contrast with the significantly elevated expression of this reporter in wild-type (Figure 3d,e,f) and TCP3SRDX plants (Figure 3d,i,j). These results suggest that expression of mTCP3 reduces auxin transport capacity and/or sensitivity
Li S, Zachgo S - TCP3 interacts with R2R3-MYB proteins, promotes flavonoid biosynthesis and negatively regulates the auxin response in Arabidopsis thaliana
Visit the PubMed ArticleTen genes involved in the flavonoid biosynthetic pathway were up-regulated 3.27- to 24.35-fold in mTCP3 plants (Table 1). These include ... structural genes encoding key flavonoid biosynthetic enzymes, such as ... LDOX/TT18
Li S, Zachgo S - TCP3 interacts with R2R3-MYB proteins, promotes flavonoid biosynthesis and negatively regulates the auxin response in Arabidopsis thaliana
Visit the PubMed ArticleTen genes involved in the flavonoid biosynthetic pathway were up-regulated 3.27- to 24.35-fold in mTCP3 plants (Table 1). These include ... structural genes encoding key flavonoid biosynthetic enzymes, such as 4CL
Li S, Zachgo S - TCP3 interacts with R2R3-MYB proteins, promotes flavonoid biosynthesis and negatively regulates the auxin response in Arabidopsis thaliana
Visit the PubMed Articlein mTCP3 plants ... Genes that negatively affect the auxin response, such as ... the auxin-responsive gene SAUR (At2 g46690), which possibly encodes a negative regulator of auxin synthesis and transport ... were up-regulated ... 2.05-fold
Li S, Zachgo S - TCP3 interacts with R2R3-MYB proteins, promotes flavonoid biosynthesis and negatively regulates the auxin response in Arabidopsis thaliana
Visit the PubMed ArticleEnhanced expression was observed for auxin-related genes in TCP3SRDX plants, such as ... SAUR68
Li S, Zachgo S - TCP3 interacts with R2R3-MYB proteins, promotes flavonoid biosynthesis and negatively regulates the auxin response in Arabidopsis thaliana
Visit the PubMed ArticleTen genes involved in the flavonoid biosynthetic pathway were up-regulated 3.27- to 24.35-fold in mTCP3 plants (Table 1). These include ... structural genes encoding key flavonoid biosynthetic enzymes, such as ... F3H/TT6
Li S, Zachgo S - TCP3 interacts with R2R3-MYB proteins, promotes flavonoid biosynthesis and negatively regulates the auxin response in Arabidopsis thaliana
Visit the PubMed Articlein the clpR4-3 proteome ... upregulated ... related to starch metabolism ... AMY3
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein the clpR4-3 proteome ... upregulated proteins ... CP12-2
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlethe clpR4-3 mutation led to a decline in proteins functioning in the Calvin–Benson cycle ... SBPase
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein thf1 ... PRX Q ... were ... downregulated
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein the clpR4-3 proteome ... FtsH2 ... was ... reduced
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlethe clpR4-3 mutation led to a decline in proteins functioning in ... amino acid biosynthesis ... GS2
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein ... 47-2 ... PRX Q ... were ... downregulated
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein ... 47-2 ... FtsH2 ... were ... downregulated
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed ArticleIn clpR4-3 ... peptidase (At5G42390 ... upregulated
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein ... clpR4-3 ... PRX Q ... were ... downregulated
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein thf1 ... upregulated ... oxidative stress ... ENH1
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed ArticleIn clpR4-3 ... RNA-binding ... CP33 ... upregulated
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein ... clpR4-3 ... CPN21 ... were ... downregulated
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed ArticleIn clpR4-3 ... ClpB3 ... upregulated
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein the clpR4-3 proteome ... upregulated proteins ... CP12-1
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein thf1 ... upregulated proteins are involved in PSI biogenesis ... PsaC
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein the clpR4-3 proteome ... FtsH8 ... was ... reduced
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein the clpR4-3 proteome ... upregulated ... CPN21 ... which may be involved in Rubisco assembly
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed ArticleIn clpR4-3 ... HSP90.5 ... upregulated
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed ArticleTo investigate the effect of the clpR4 mutation on plastid rRNA processing, we examined levels of rRNAs (23S, 16S, 5S, and 4.5S) using RNA blotting with gene-specific probes. The plastid rrn operon shown in Figure 5(a) is transcribed as a large polycistronic precursor RNA, which is processed subsequently by a series of endo- and exo-ribonucleases to yield mature rRNAs and tRNAs (Harris et al., 1994a). Except for mature 5S and 16S rRNAs, 4.5S and three mature 23S (1.2-, 1.0- and 0.5-kb) rRNAs were clearly reduced in ... 47-2 mutants ... compared with WT
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein ... 47-2 ... CPN21 ... were ... downregulated
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed ArticleIn clpR4-3 ... RPL12C ... downregulated
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed ArticleImmunoblotting analysis showed that VAR2 accumulation was apparently increased in 47-2 compared with thf1
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed ArticleFe-SOD ... involved in antioxidative defense ... downregulated in clpR4-3
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein thf1 ... downregulated proteins ... carotenoid metabolism ... CCD4
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed ArticleIn clpR4-3 ... AT5G24490 ... downregulated
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed ArticleGSTF8 ... involved in antioxidative defense ... downregulated in clpR4-3
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein thf1 ... FtsH2 ... were ... downregulated
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed ArticleThe levels of ... FtsH8 ... were dramatically reduced in thf1
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein the clpR4-3 proteome ... OE33/PsbO1 ... in the PSII oxygen-evolving complex ... downregulated
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein thf1 ... upregulated ... CP12-1
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed ArticlePRX Q ... involved in antioxidative defense ... downregulated in clpR4-3
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein ... clpR4-3 ... FtsH8 ... were ... downregulated
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlethe clpR4-3 mutation led to a decline in proteins functioning in ... amino acid biosynthesis ... His-HF
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein ... clpR4-3 ... CP12-1 ... were ... downregulated
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein thf1 ... downregulated proteins are associated with oxidative defense ... PRX Q
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein the clpR4-3 proteome ... upregulated ... related to ... chlorophyll biosynthesis ... HEMB
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein ... 47-2 ... THF1 ... were ... downregulated
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed ArticleIn clpR4-3 ... Cpn60B2 ... upregulated
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed ArticleIn clpR4-3 ... RPL1 ... downregulated
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein the clpR4-3 proteome ... PetA in the cytochrome b6f complex ... downregulated
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein ... 47-2 ... CP12-1 ... were ... downregulated
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed ArticleIn clpR4-3 ... RNA-binding ... RBP29 ... upregulated
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed ArticleIn clpR4-3 ... ClpR2, was also down-regulated by about three-fold
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein thf1 ... downregulated ... protein folding in the thylakoid lumen ... FKBP13
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed ArticleThe levels of ... FtsH5/VAR1 were dramatically reduced in thf1
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein the clpR4-3 proteome ... upregulated ... related to starch metabolism ... SBE2.2
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein thf1 ... CPN21 ... were ... downregulated
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed ArticleIn clpR4-3 ... exoribonuclease (PNPase) that mediates global RNA decay ... upregulated
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein thf1 ... upregulated ... plastid protein translation ... PSRP2
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein thf1 ... upregulated ... CPN21/GroES
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein ... 47-2 ... FtsH8 ... were ... downregulated
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Article47-2 ... accumulated higher levels of several rRNA precursors, e.g. 4.5S plus 23S (23S 3.2-kb fragment) and 16S precursors, than WT
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein thf1 ... upregulated ... oxidative stress ... PRXII E
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed ArticleIn clpR4-3 ... RNA-binding ... RBP31 ... upregulated
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed ArticleTo investigate the effect of the clpR4 mutation on plastid rRNA processing, we examined levels of rRNAs (23S, 16S, 5S, and 4.5S) using RNA blotting with gene-specific probes. The plastid rrn operon shown in Figure 5(a) is transcribed as a large polycistronic precursor RNA, which is processed subsequently by a series of endo- and exo-ribonucleases to yield mature rRNAs and tRNAs (Harris et al., 1994a). Except for mature 5S and 16S rRNAs, 4.5S and three mature 23S (1.2-, 1.0- and 0.5-kb) rRNAs were clearly reduced in clpR4-3 ... compared with WT
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein thf1 ... downregulated ... plastid gene transcription ... PTAC16
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein thf1 ... downregulated proteins are associated with oxidative defense ... DHAR3
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlethe clpR4-3 mutation led to a decline in proteins functioning in ... nucleotide metabolism ... AMK2
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein thf1 ... FtsH8 ... were ... downregulated
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein the clpR4-3 proteome ... upregulated ... jasmonic acid biosynthesis ... LOX2
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlethe clpR4-3 mutation led to a decline in proteins functioning in ... de novo fatty acid biosynthesis ... CAC3
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed ArticleDHAR3 ... involved in antioxidative defense ... downregulated in clpR4-3
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein thf1 ... downregulated ... amino acid biosynthesis ... ASP5
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlethf1 produced the same levels of rRNAs as WT
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein the clpR4-3 proteome ... PsbQ in the PSII oxygen-evolving complex ... downregulated
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein the clpR4-3 proteome ... VIPP1 required for the assembly of the photosynthetic apparatus in collaboration with Alb3 ... downregulated
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed ArticleIn clpR4-3 ... translation elongation factors ... EF-Ts ... upregulated
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed ArticleThe GFP signal was overlapped with the chlorophyll autofluorescence, indicating that ClpR4 is localized in chloroplasts
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein ... clpR4-3 ... THF1 ... were ... downregulated
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein thf1 ... CP12-1 ... were ... downregulated
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein the clpR4-3 proteome ... upregulated ... ABA-mediated photoprotection ... PGL35
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed ArticleIn clpR4-3 ... translation elongation factor ... EF-Tu ... upregulated
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed ArticleIn clpR4-3 ... translation elongation factor ... SCO/EF-G ... upregulated
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlethe clpR4-3 mutation led to a decline in proteins functioning in the Calvin–Benson cycle ... RPE
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlethe clpR4-3 mutation led to a decline in proteins functioning in the Calvin–Benson cycle ... RBCL
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein the clpR4-3 proteome ... upregulated ... methylerythritol phosphate (MEP) pathway (ISPG
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein ... clpR4-3 ... FtsH2 ... were ... downregulated
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed ArticleThe levels of FtsH2/VAR2 ... were dramatically reduced in thf1
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein the clpR4-3 proteome ... PsbS ... was ... reduced
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed ArticlePRX M1 ... involved in antioxidative defense ... downregulated in clpR4-3
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed ArticleclpR4-3 ... accumulated higher levels of several rRNA precursors, e.g. 4.5S plus 23S (23S 3.2-kb fragment) and 16S precursors, than WT
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein the clpR4-3 proteome ... THF1 ... was ... reduced
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed Articlein thf1 ... THF1 ... were ... downregulated
Wu W, Zhu Y, Ma Z, Sun Y, Quan Q, Li P, Hu P, Shi T, Lo C, Chu IK, Huang J - Proteomic evidence for genetic epistasis: ClpR4 mutations switch leaf variegation to virescence in Arabidopsis
Visit the PubMed ArticleFLAG–PDF2 fusions were generated under the control of the PDF1 promoter ... ATML1 ... significantly down-regulated in PDF2 over-expressing plants
San-Bento R, Farcot E, Galletti R, Creff A, Ingram G - Epidermal identity is maintained by cell-cell communication via a universally active feedback loop in Arabidopsis thaliana
Visit the PubMed Articleale1-4 mutant seedlings ... We found no change in the expression of ... PDF2
San-Bento R, Farcot E, Galletti R, Creff A, Ingram G - Epidermal identity is maintained by cell-cell communication via a universally active feedback loop in Arabidopsis thaliana
Visit the PubMed Articlegso-1 gso2-1 double mutant ... We found no change in the expression of ATML1
San-Bento R, Farcot E, Galletti R, Creff A, Ingram G - Epidermal identity is maintained by cell-cell communication via a universally active feedback loop in Arabidopsis thaliana
Visit the PubMed ArticleLevels of ACR4 expression in ... pdf2-2 ... were not significantly different to those in wild-type plants
San-Bento R, Farcot E, Galletti R, Creff A, Ingram G - Epidermal identity is maintained by cell-cell communication via a universally active feedback loop in Arabidopsis thaliana
Visit the PubMed ArticleLevels of ACR4 expression in ... atml1-3 ... were not significantly different to those in wild-type plants
San-Bento R, Farcot E, Galletti R, Creff A, Ingram G - Epidermal identity is maintained by cell-cell communication via a universally active feedback loop in Arabidopsis thaliana
Visit the PubMed Articlegso-1 gso2-1 double mutant ... We found no change in the expression of ... ACR4
San-Bento R, Farcot E, Galletti R, Creff A, Ingram G - Epidermal identity is maintained by cell-cell communication via a universally active feedback loop in Arabidopsis thaliana
Visit the PubMed Articlewe quantified the expression levels of ATML1 ... in acr4-2 mutant seedlings ... A slight but significant decrease in the expression levels ... was observed in acr4-2 mutants compared to wild-type seedlings
San-Bento R, Farcot E, Galletti R, Creff A, Ingram G - Epidermal identity is maintained by cell-cell communication via a universally active feedback loop in Arabidopsis thaliana
Visit the PubMed Articlewe quantified the expression levels of ... PDF2 in acr4-2 mutant seedlings. A slight but significant decrease in the expression levels ... was observed in acr4-2 mutants compared to wild-type seedlings
San-Bento R, Farcot E, Galletti R, Creff A, Ingram G - Epidermal identity is maintained by cell-cell communication via a universally active feedback loop in Arabidopsis thaliana
Visit the PubMed Articleale1-4 mutant seedlings ... We found no change in the expression of ATML1
San-Bento R, Farcot E, Galletti R, Creff A, Ingram G - Epidermal identity is maintained by cell-cell communication via a universally active feedback loop in Arabidopsis thaliana
Visit the PubMed Articleale1-4 mutant seedlings ... We found no change in the expression of ... ACR4
San-Bento R, Farcot E, Galletti R, Creff A, Ingram G - Epidermal identity is maintained by cell-cell communication via a universally active feedback loop in Arabidopsis thaliana
Visit the PubMed ArticleFLAG–PDF2 fusions were generated under the control of the PDF1 promoter ... PDF2 ... significantly down-regulated in PDF2 over-expressing plants
San-Bento R, Farcot E, Galletti R, Creff A, Ingram G - Epidermal identity is maintained by cell-cell communication via a universally active feedback loop in Arabidopsis thaliana
Visit the PubMed Articlegso-1 gso2-1 double mutant ... We found no change in the expression of ... PDF2
San-Bento R, Farcot E, Galletti R, Creff A, Ingram G - Epidermal identity is maintained by cell-cell communication via a universally active feedback loop in Arabidopsis thaliana
Visit the PubMed Articlein the progeny of a pdf2-2 mutant additionally heterozygous for atml1-3, a small but significant increase in ACR4 expression levels was consistently observed
San-Bento R, Farcot E, Galletti R, Creff A, Ingram G - Epidermal identity is maintained by cell-cell communication via a universally active feedback loop in Arabidopsis thaliana
Visit the PubMed ArticleFLAG–PDF2 fusions were generated under the control of the PDF1 promoter ... Expression of ACR4 in backgrounds over-expressing FLAG–PDF2 was significantly decreased compared to that in wild-type plants, again consistent with negative feedback by PDF2 on the ACR4 promoter
San-Bento R, Farcot E, Galletti R, Creff A, Ingram G - Epidermal identity is maintained by cell-cell communication via a universally active feedback loop in Arabidopsis thaliana
Visit the PubMed Articleno significant transcript changes were detected for ... CAT2 ... in cls1
Pineau B, Bourge M, Marion J, Mauve C, Gilard F, Maneta-Peyret L, Moreau P, Satiat-Jeunemaître B, Brown SC, De Paepe R, Danon A - The importance of cardiolipin synthase for mitochondrial ultrastructure, respiratory function, plant development, and stress responses in Arabidopsis
Visit the PubMed ArticleAPX2 transcript levels were markedly increased in cls1
Pineau B, Bourge M, Marion J, Mauve C, Gilard F, Maneta-Peyret L, Moreau P, Satiat-Jeunemaître B, Brown SC, De Paepe R, Danon A - The importance of cardiolipin synthase for mitochondrial ultrastructure, respiratory function, plant development, and stress responses in Arabidopsis
Visit the PubMed ArticleIn cls1, transcript levels of BAP1 ... were strongly increased, suggesting singlet oxygen but not superoxide/hydrogen peroxide overproduction in cls1 plastids
Pineau B, Bourge M, Marion J, Mauve C, Gilard F, Maneta-Peyret L, Moreau P, Satiat-Jeunemaître B, Brown SC, De Paepe R, Danon A - The importance of cardiolipin synthase for mitochondrial ultrastructure, respiratory function, plant development, and stress responses in Arabidopsis
Visit the PubMed Articletranscript levels of AOX1 ... encoding alternative oxidase ... markedly increased in cls1 seedlings
Pineau B, Bourge M, Marion J, Mauve C, Gilard F, Maneta-Peyret L, Moreau P, Satiat-Jeunemaître B, Brown SC, De Paepe R, Danon A - The importance of cardiolipin synthase for mitochondrial ultrastructure, respiratory function, plant development, and stress responses in Arabidopsis
Visit the PubMed ArticleCAT3 ... transcript levels were markedly increased in cls1
Pineau B, Bourge M, Marion J, Mauve C, Gilard F, Maneta-Peyret L, Moreau P, Satiat-Jeunemaître B, Brown SC, De Paepe R, Danon A - The importance of cardiolipin synthase for mitochondrial ultrastructure, respiratory function, plant development, and stress responses in Arabidopsis
Visit the PubMed Articlein cls1 ... the accumulation of complex IV, revealed by the anti-COXII antibody, was unaffected
Pineau B, Bourge M, Marion J, Mauve C, Gilard F, Maneta-Peyret L, Moreau P, Satiat-Jeunemaître B, Brown SC, De Paepe R, Danon A - The importance of cardiolipin synthase for mitochondrial ultrastructure, respiratory function, plant development, and stress responses in Arabidopsis
Visit the PubMed Articletranscript levels of ... NDB2 ... external NAD(P)H dehydrogenase ... markedly increased in cls1 seedlings
Pineau B, Bourge M, Marion J, Mauve C, Gilard F, Maneta-Peyret L, Moreau P, Satiat-Jeunemaître B, Brown SC, De Paepe R, Danon A - The importance of cardiolipin synthase for mitochondrial ultrastructure, respiratory function, plant development, and stress responses in Arabidopsis
Visit the PubMed Articleno significant transcript changes were detected for ... FER1 ... in cls1
Pineau B, Bourge M, Marion J, Mauve C, Gilard F, Maneta-Peyret L, Moreau P, Satiat-Jeunemaître B, Brown SC, De Paepe R, Danon A - The importance of cardiolipin synthase for mitochondrial ultrastructure, respiratory function, plant development, and stress responses in Arabidopsis
Visit the PubMed ArticleWe compared the accumulation of respiratory CI and CI/CIII in Col-0 and the cls1 mutant by blue native electrophoresis (Figure 6). In dodecyl maltoside–solubilized proteins of Col-0 and cls1 leaf membrane extracts (Figure 6A), both in-gel NADH/nitroblue tetrazolium (NBT) staining, which reveals NADH dehydrogenase activity, and immune detection using an antibody directed against the NAD9 subunit of the CI peripheral arm (Klodmann et al., 2010) showed that CI accumulation was lower in cls1 than in Col-0
Pineau B, Bourge M, Marion J, Mauve C, Gilard F, Maneta-Peyret L, Moreau P, Satiat-Jeunemaître B, Brown SC, De Paepe R, Danon A - The importance of cardiolipin synthase for mitochondrial ultrastructure, respiratory function, plant development, and stress responses in Arabidopsis
Visit the PubMed ArticleCAT1 ... transcript levels were markedly increased in cls1
Pineau B, Bourge M, Marion J, Mauve C, Gilard F, Maneta-Peyret L, Moreau P, Satiat-Jeunemaître B, Brown SC, De Paepe R, Danon A - The importance of cardiolipin synthase for mitochondrial ultrastructure, respiratory function, plant development, and stress responses in Arabidopsis
Visit the PubMed Articleno significant transcript changes were detected for ... APX1 ... in cls1
Pineau B, Bourge M, Marion J, Mauve C, Gilard F, Maneta-Peyret L, Moreau P, Satiat-Jeunemaître B, Brown SC, De Paepe R, Danon A - The importance of cardiolipin synthase for mitochondrial ultrastructure, respiratory function, plant development, and stress responses in Arabidopsis
Visit the PubMed Article35S:KAN1-GR ... most instances down-regulation was observed at both time points, with 43 genes down-regulated only at 160 minutes and 4 genes down-regulated only at 80 minutes. Of the down-regulated genes, 42 are known to have a role in auxin biology ... PIN4
Merelo P, Xie Y, Brand L, Ott F, Weigel D, Bowman JL, Heisler MG, Wenkel S - Genome-wide identification of KANADI1 target genes
Visit the PubMed Article35S:KAN1-GR ... most instances down-regulation was observed at both time points, with 43 genes down-regulated only at 160 minutes and 4 genes down-regulated only at 80 minutes. Of the down-regulated genes, 42 are known to have a role in auxin biology ... ARF19
Merelo P, Xie Y, Brand L, Ott F, Weigel D, Bowman JL, Heisler MG, Wenkel S - Genome-wide identification of KANADI1 target genes
Visit the PubMed Article35S:KAN1-GR ... most instances down-regulation was observed at both time points, with 43 genes down-regulated only at 160 minutes and 4 genes down-regulated only at 80 minutes. Of the down-regulated genes, 42 are known to have a role in auxin biology ... PID
Merelo P, Xie Y, Brand L, Ott F, Weigel D, Bowman JL, Heisler MG, Wenkel S - Genome-wide identification of KANADI1 target genes
Visit the PubMed Article35S:KAN1-GR ... most instances down-regulation was observed at both time points, with 43 genes down-regulated only at 160 minutes and 4 genes down-regulated only at 80 minutes. Of the down-regulated genes, 42 are known to have a role in auxin biology ... PIN1
Merelo P, Xie Y, Brand L, Ott F, Weigel D, Bowman JL, Heisler MG, Wenkel S - Genome-wide identification of KANADI1 target genes
Visit the PubMed Article35S:KAN1-GR ... most instances down-regulation was observed at both time points, with 43 genes down-regulated only at 160 minutes and 4 genes down-regulated only at 80 minutes. Of the down-regulated genes, 42 are known to have a role in auxin biology ... ARF4
Merelo P, Xie Y, Brand L, Ott F, Weigel D, Bowman JL, Heisler MG, Wenkel S - Genome-wide identification of KANADI1 target genes
Visit the PubMed Article35S:KAN1-GR ... most instances down-regulation was observed at both time points, with 43 genes down-regulated only at 160 minutes and 4 genes down-regulated only at 80 minutes. Of the down-regulated genes, 42 are known to have a role in auxin biology ... AUX1
Merelo P, Xie Y, Brand L, Ott F, Weigel D, Bowman JL, Heisler MG, Wenkel S - Genome-wide identification of KANADI1 target genes
Visit the PubMed Article35S:KAN1-GR ... most instances down-regulation was observed at both time points, with 43 genes down-regulated only at 160 minutes and 4 genes down-regulated only at 80 minutes. Of the down-regulated genes, 42 are known to have a role in auxin biology ... PIN7
Merelo P, Xie Y, Brand L, Ott F, Weigel D, Bowman JL, Heisler MG, Wenkel S - Genome-wide identification of KANADI1 target genes
Visit the PubMed Article35S:KAN1-GR ... most instances down-regulation was observed at both time points, with 43 genes down-regulated only at 160 minutes and 4 genes down-regulated only at 80 minutes. Of the down-regulated genes, 42 are known to have a role in auxin biology ... PIN3
Merelo P, Xie Y, Brand L, Ott F, Weigel D, Bowman JL, Heisler MG, Wenkel S - Genome-wide identification of KANADI1 target genes
Visit the PubMed Article35S:KAN1-GR ... down-regulated were 102 genes implicated in transcriptional regulation (Dataset S5), including some previously implicated in regulation of leaf polarity ... PHB
Merelo P, Xie Y, Brand L, Ott F, Weigel D, Bowman JL, Heisler MG, Wenkel S - Genome-wide identification of KANADI1 target genes
Visit the PubMed Article35S:KAN1-GR ... most instances down-regulation was observed at both time points, with 43 genes down-regulated only at 160 minutes and 4 genes down-regulated only at 80 minutes. Of the down-regulated genes, 42 are known to have a role in auxin biology ... IAA2
Merelo P, Xie Y, Brand L, Ott F, Weigel D, Bowman JL, Heisler MG, Wenkel S - Genome-wide identification of KANADI1 target genes
Visit the PubMed Article35S:KAN1-GR ... most instances down-regulation was observed at both time points, with 43 genes down-regulated only at 160 minutes and 4 genes down-regulated only at 80 minutes. Of the down-regulated genes, 42 are known to have a role in auxin biology ... PGP19
Merelo P, Xie Y, Brand L, Ott F, Weigel D, Bowman JL, Heisler MG, Wenkel S - Genome-wide identification of KANADI1 target genes
Visit the PubMed Article35S:KAN1-GR ... most instances down-regulation was observed at both time points, with 43 genes down-regulated only at 160 minutes and 4 genes down-regulated only at 80 minutes. Of the down-regulated genes, 42 are known to have a role in auxin biology ... PGP4
Merelo P, Xie Y, Brand L, Ott F, Weigel D, Bowman JL, Heisler MG, Wenkel S - Genome-wide identification of KANADI1 target genes
Visit the PubMed Article35S:KAN1-GR ... most instances down-regulation was observed at both time points, with 43 genes down-regulated only at 160 minutes and 4 genes down-regulated only at 80 minutes. Of the down-regulated genes, 42 are known to have a role in auxin biology ... IAA13
Merelo P, Xie Y, Brand L, Ott F, Weigel D, Bowman JL, Heisler MG, Wenkel S - Genome-wide identification of KANADI1 target genes
Visit the PubMed Article35S:KAN1-GR ... most instances down-regulation was observed at both time points, with 43 genes down-regulated only at 160 minutes and 4 genes down-regulated only at 80 minutes. Of the down-regulated genes, 42 are known to have a role in auxin biology ... IAA14
Merelo P, Xie Y, Brand L, Ott F, Weigel D, Bowman JL, Heisler MG, Wenkel S - Genome-wide identification of KANADI1 target genes
Visit the PubMed Article35S:KAN1-GR ... most instances down-regulation was observed at both time points, with 43 genes down-regulated only at 160 minutes and 4 genes down-regulated only at 80 minutes. Of the down-regulated genes, 42 are known to have a role in auxin biology ... IAA16
Merelo P, Xie Y, Brand L, Ott F, Weigel D, Bowman JL, Heisler MG, Wenkel S - Genome-wide identification of KANADI1 target genes
Visit the PubMed Article35S:KAN1-GR ... most instances down-regulation was observed at both time points, with 43 genes down-regulated only at 160 minutes and 4 genes down-regulated only at 80 minutes. Of the down-regulated genes, 42 are known to have a role in auxin biology ... HAT2
Merelo P, Xie Y, Brand L, Ott F, Weigel D, Bowman JL, Heisler MG, Wenkel S - Genome-wide identification of KANADI1 target genes
Visit the PubMed Article35S:KAN1-GR ... most instances down-regulation was observed at both time points, with 43 genes down-regulated only at 160 minutes and 4 genes down-regulated only at 80 minutes. Of the down-regulated genes, 42 are known to have a role in auxin biology ... BIG
Merelo P, Xie Y, Brand L, Ott F, Weigel D, Bowman JL, Heisler MG, Wenkel S - Genome-wide identification of KANADI1 target genes
Visit the PubMed Article35S:KAN1-GR ... most instances down-regulation was observed at both time points, with 43 genes down-regulated only at 160 minutes and 4 genes down-regulated only at 80 minutes. Of the down-regulated genes, 42 are known to have a role in auxin biology ... IAA3
Merelo P, Xie Y, Brand L, Ott F, Weigel D, Bowman JL, Heisler MG, Wenkel S - Genome-wide identification of KANADI1 target genes
Visit the PubMed Article35S:KAN1-GR ... down-regulated were 102 genes implicated in transcriptional regulation (Dataset S5), including some previously implicated in regulation of leaf polarity ... YABBY5
Merelo P, Xie Y, Brand L, Ott F, Weigel D, Bowman JL, Heisler MG, Wenkel S - Genome-wide identification of KANADI1 target genes
Visit the PubMed ArticleWe also treated plants with the BIN2 inhibitor bikinin and found that phosphorylated HAT1 protein level decreased significantly compared with the mock control ... It is worth noting that although HAT1 appears to increase at 1 h after bikinin treatment, its relative level actually decreases after normalizing with the mock control in which HAT1 increased more at the same time point
Zhang D, Ye H, Guo H, Johnson A, Zhang M, Lin H, Yin Y - Transcription factor HAT1 is phosphorylated by BIN2 kinase and mediates brassinosteroid repressed gene expression in Arabidopsis
Visit the PubMed ArticleWe then performed transient gene expression with DWF4 promoter::luciferase (LUC) reporter gene in tobacco leaves. While expression of BES1 ... repressed the expression of DWF4pro:LUC gene expression ... the expression of the reporter gene was further reduced when both BES1 and HAT1 were co-expressed
Zhang D, Ye H, Guo H, Johnson A, Zhang M, Lin H, Yin Y - Transcription factor HAT1 is phosphorylated by BIN2 kinase and mediates brassinosteroid repressed gene expression in Arabidopsis
Visit the PubMed ArticleWe also treated plants with the BIN2 inhibitor bikinin ... BES1 was dephosphorylated and accumulated with bikinin treatment
Zhang D, Ye H, Guo H, Johnson A, Zhang M, Lin H, Yin Y - Transcription factor HAT1 is phosphorylated by BIN2 kinase and mediates brassinosteroid repressed gene expression in Arabidopsis
Visit the PubMed ArticleThe HAT1 protein level was reduced in plants grown in medium with BL
Zhang D, Ye H, Guo H, Johnson A, Zhang M, Lin H, Yin Y - Transcription factor HAT1 is phosphorylated by BIN2 kinase and mediates brassinosteroid repressed gene expression in Arabidopsis
Visit the PubMed Articlein bes1-D, in which the BES1 protein level is increased, the expression of HAT1 is reduced to 18% without exogenous BL and even more reduced with BL treatment
Zhang D, Ye H, Guo H, Johnson A, Zhang M, Lin H, Yin Y - Transcription factor HAT1 is phosphorylated by BIN2 kinase and mediates brassinosteroid repressed gene expression in Arabidopsis
Visit the PubMed ArticleWe then performed transient gene expression with DWF4 promoter::luciferase (LUC) reporter gene in tobacco leaves. While expression of ... HAT1 repressed the expression of DWF4pro:LUC gene expression, the expression of the reporter gene was further reduced when both BES1 and HAT1 were co-expressed
Zhang D, Ye H, Guo H, Johnson A, Zhang M, Lin H, Yin Y - Transcription factor HAT1 is phosphorylated by BIN2 kinase and mediates brassinosteroid repressed gene expression in Arabidopsis
Visit the PubMed Articlewe performed quantitative reverse transcription polymerase chain reaction (qRT-PCR) experiments ... expression was decreased ... in wild-type (WT) seedlings after BL treatment ... expression of HAT3
Zhang D, Ye H, Guo H, Johnson A, Zhang M, Lin H, Yin Y - Transcription factor HAT1 is phosphorylated by BIN2 kinase and mediates brassinosteroid repressed gene expression in Arabidopsis
Visit the PubMed Articlewe performed quantitative reverse transcription polymerase chain reaction (qRT-PCR) experiments (Figure S1). HAT1 expression was decreased to about 35% in wild-type (WT) seedlings after BL treatment
Zhang D, Ye H, Guo H, Johnson A, Zhang M, Lin H, Yin Y - Transcription factor HAT1 is phosphorylated by BIN2 kinase and mediates brassinosteroid repressed gene expression in Arabidopsis
Visit the PubMed ArticleWhen using anti–GFP antibody to test the expression of HAT1–GFP in transgenic plants (Figure 2d), two HAT1–GFP bands were detected. To investigate whether HAT1 exists as phosphorylated and/or unphosphorylated forms in plants, HAT1–GFP was immunoprecipitated from HAT1–GFPOX transgenic plants and followed by calf intestinal phosphatase (CIP) treatment (Figures 4a, S7 and S8). The top two bands disappeared and a new lower band, probably the unphosphorylated form of HAT1, appeared after CIP treatment. The results indicated that HAT1 exists mostly as phosphorylated forms in plants and the unphosphorylated HAT1 is barely detectable under normal growth conditions
Zhang D, Ye H, Guo H, Johnson A, Zhang M, Lin H, Yin Y - Transcription factor HAT1 is phosphorylated by BIN2 kinase and mediates brassinosteroid repressed gene expression in Arabidopsis
Visit the PubMed Articlewe performed quantitative reverse transcription polymerase chain reaction (qRT-PCR) experiments ... in bes1-D, in which the BES1 protein level is increased, the expression ... is reduced ... without exogenous BL and even more reduced with BL treatment ... expression of HAT3
Zhang D, Ye H, Guo H, Johnson A, Zhang M, Lin H, Yin Y - Transcription factor HAT1 is phosphorylated by BIN2 kinase and mediates brassinosteroid repressed gene expression in Arabidopsis
Visit the PubMed ArticleWe found that HAT1 protein increases in the liquid MS (Murashige and Skoog) medium without BL treatment (Figures 4c top panel and S9). In contrast, HAT1 protein clearly decreased in relation to the mock treatment in the presence of 1 µm BL ... BL induces accumulation of unphosphorylated BES1
Zhang D, Ye H, Guo H, Johnson A, Zhang M, Lin H, Yin Y - Transcription factor HAT1 is phosphorylated by BIN2 kinase and mediates brassinosteroid repressed gene expression in Arabidopsis
Visit the PubMed ArticleWhen we treated plants with BL and MG132 together, the HAT1 protein level significantly increased and the unphosphorylated HAT1 also appeared
Zhang D, Ye H, Guo H, Johnson A, Zhang M, Lin H, Yin Y - Transcription factor HAT1 is phosphorylated by BIN2 kinase and mediates brassinosteroid repressed gene expression in Arabidopsis
Visit the PubMed ArticleThe HAT1 protein level was ... increased in plants grown in medium with BRZ
Zhang D, Ye H, Guo H, Johnson A, Zhang M, Lin H, Yin Y - Transcription factor HAT1 is phosphorylated by BIN2 kinase and mediates brassinosteroid repressed gene expression in Arabidopsis
Visit the PubMed Articleno apparent difference in the outer envelope marker Toc75 was seen between WT and abc1k1/pgr6 plants
Martinis J, Glauser G, Valimareanu S, Stettler M, Zeeman SC, Yamamoto H, Shikanai T, Kessler F - ABC1K1/PGR6 kinase: a regulatory link between photosynthetic activity and chloroplast metabolism
Visit the PubMed ArticleVTE1 was detected in WT PGs but not in those of the abc1k1/pgr6 single mutant, comparable with the mutant lacking the PG kinase ABC1K3
Martinis J, Glauser G, Valimareanu S, Stettler M, Zeeman SC, Yamamoto H, Shikanai T, Kessler F - ABC1K1/PGR6 kinase: a regulatory link between photosynthetic activity and chloroplast metabolism
Visit the PubMed ArticleTo establish whether VTE1 was affected at the level of gene expression or post-transcriptionally, quantitative RT-PCR was performed on total RNA extracted from fresh WT and abc1k1/pgr6 leaves. A significant increase (2.5-fold) of VTE1 transcript was detected in abc1k1/pgr6 when compared with WT plants (Figure 6b), an observation similar to that for the abc1k3 single mutant
Martinis J, Glauser G, Valimareanu S, Stettler M, Zeeman SC, Yamamoto H, Shikanai T, Kessler F - ABC1K1/PGR6 kinase: a regulatory link between photosynthetic activity and chloroplast metabolism
Visit the PubMed ArticleEquivalent protein quantities of chloroplast membrane subfractions (PG; envelopes, ENV; thylakoids, THY) were separated by SDS-PAGE, transferred to nitrocellulose and colored with Amido black ... ABC1K1 was detectable only in the PG fraction purified from WT plants
Martinis J, Glauser G, Valimareanu S, Stettler M, Zeeman SC, Yamamoto H, Shikanai T, Kessler F - ABC1K1/PGR6 kinase: a regulatory link between photosynthetic activity and chloroplast metabolism
Visit the PubMed ArticleEquivalent protein quantities of chloroplast membrane subfractions (PG; envelopes, ENV; thylakoids, THY) were separated by SDS-PAGE, transferred to nitrocellulose and colored with Amido black ... VTE1 was detected in WT PGs
Martinis J, Glauser G, Valimareanu S, Stettler M, Zeeman SC, Yamamoto H, Shikanai T, Kessler F - ABC1K1/PGR6 kinase: a regulatory link between photosynthetic activity and chloroplast metabolism
Visit the PubMed Articlea reduction of chlorophyll binding protein LHCb2 was observed in abc1k1/pgr6 thylakoids
Martinis J, Glauser G, Valimareanu S, Stettler M, Zeeman SC, Yamamoto H, Shikanai T, Kessler F - ABC1K1/PGR6 kinase: a regulatory link between photosynthetic activity and chloroplast metabolism
Visit the PubMed ArticleConcerning the other proteins investigated by immunoblotting (Figure 6a), the aFBN1a serum detected two distinct proteins with an apparent molecular mass just above 30 kDa, and likely to be identical with the two fibrillins FBN1a and FBN1b, as previously reported ... the lower band (FBN1b) was significantly reduced in abc1k1/pgr6 PGs when compared with WT plants
Martinis J, Glauser G, Valimareanu S, Stettler M, Zeeman SC, Yamamoto H, Shikanai T, Kessler F - ABC1K1/PGR6 kinase: a regulatory link between photosynthetic activity and chloroplast metabolism
Visit the PubMed ArticleConcerning the other proteins investigated by immunoblotting (Figure 6a), the aFBN1a serum detected two distinct proteins with an apparent molecular mass just above 30 kDa, and likely to be identical with the two fibrillins FBN1a and FBN1b, as previously reported ... the upper band (FBN1a) was not detectable ... in abc1k1/pgr6 PGs when compared with WT plants
Martinis J, Glauser G, Valimareanu S, Stettler M, Zeeman SC, Yamamoto H, Shikanai T, Kessler F - ABC1K1/PGR6 kinase: a regulatory link between photosynthetic activity and chloroplast metabolism
Visit the PubMed Articlethe fibrillin FBN2 was much less abundant in abc1k1/pgr6 PGs than in the WT
Martinis J, Glauser G, Valimareanu S, Stettler M, Zeeman SC, Yamamoto H, Shikanai T, Kessler F - ABC1K1/PGR6 kinase: a regulatory link between photosynthetic activity and chloroplast metabolism
Visit the PubMed ArticleBoth HA–TWD1 and HA–TWD1-Ct detected by anti-HA also partially overlapped with the ER marker binding Protein although at reduced molecular weight consistent with a previously published ER location
Bailly A, Wang B, Zwiewka M, Pollmann S, Schenck D, Lüthen H, Schulz A, Friml J, Geisler M - Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth
Visit the PubMed Articleimmunodetection of HA–TWD1-Ct that lacked the C-terminal membrane anchor resulted in a stronger but similar plasma membrane pattern to the wild type
Bailly A, Wang B, Zwiewka M, Pollmann S, Schenck D, Lüthen H, Schulz A, Friml J, Geisler M - Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth
Visit the PubMed Articlethe presence of both TWD1-OX at the PM was verified by western blot analysis of sucrose gradient fractions using anti-TWD1 and anti-HA and revealed that HA–TWD1 and HA–TWD1-Ct overlap with the PM markers plasma membrane-intrinsic protein and H+-ATPase
Bailly A, Wang B, Zwiewka M, Pollmann S, Schenck D, Lüthen H, Schulz A, Friml J, Geisler M - Expression of TWISTED DWARF1 lacking its in-plane membrane anchor leads to increased cell elongation and hypermorphic growth
Visit the PubMed Articlein cue1, the ORE1 transcript level was increased even in the absence of nitroprusside treatment
Du J, Li M, Kong D, Wang L, Lv Q, Wang J, Bao F, Gong Q, Xia J, He Y - Nitric oxide induces cotyledon senescence involving co-operation of the NES1/MAD1 and EIN2-associated ORE1 signalling pathways in Arabidopsis
Visit the PubMed ArticleTo examine the expression of NES1 further, quantitative real-time RT-PCR was used, which allowed the detection of the native message. NES1 was expressed in all organs
Du J, Li M, Kong D, Wang L, Lv Q, Wang J, Bao F, Gong Q, Xia J, He Y - Nitric oxide induces cotyledon senescence involving co-operation of the NES1/MAD1 and EIN2-associated ORE1 signalling pathways in Arabidopsis
Visit the PubMed ArticleWhen 5-day-old seedlings were treated with 20 μM nitroprusside, the message level of NES1 in the cotyledons rose linearly within 12h and then remained steady up to at least 72h
Du J, Li M, Kong D, Wang L, Lv Q, Wang J, Bao F, Gong Q, Xia J, He Y - Nitric oxide induces cotyledon senescence involving co-operation of the NES1/MAD1 and EIN2-associated ORE1 signalling pathways in Arabidopsis
Visit the PubMed ArticleTo examine the expression of NES1, transgenic plants carrying a native NES1 promoter–GUS gene fusion were first prepared ... The GUS expression was strongly increased when 5-day-old seedlings were treated with 20 μM nitroprusside for 2 d
Du J, Li M, Kong D, Wang L, Lv Q, Wang J, Bao F, Gong Q, Xia J, He Y - Nitric oxide induces cotyledon senescence involving co-operation of the NES1/MAD1 and EIN2-associated ORE1 signalling pathways in Arabidopsis
Visit the PubMed ArticleThe NES1 transcript level was slightly higher in the ein2 mutant than that in the ore1 mutant and the wild type
Du J, Li M, Kong D, Wang L, Lv Q, Wang J, Bao F, Gong Q, Xia J, He Y - Nitric oxide induces cotyledon senescence involving co-operation of the NES1/MAD1 and EIN2-associated ORE1 signalling pathways in Arabidopsis
Visit the PubMed Articlewith nitric oxide for 24h ... in nes1-2 ... down-regulation of CAB2 was apparent ... even more
Du J, Li M, Kong D, Wang L, Lv Q, Wang J, Bao F, Gong Q, Xia J, He Y - Nitric oxide induces cotyledon senescence involving co-operation of the NES1/MAD1 and EIN2-associated ORE1 signalling pathways in Arabidopsis
Visit the PubMed Articlewith nitric oxide for 24h ... down-regulation of CAB2 was apparent in the wild type
Du J, Li M, Kong D, Wang L, Lv Q, Wang J, Bao F, Gong Q, Xia J, He Y - Nitric oxide induces cotyledon senescence involving co-operation of the NES1/MAD1 and EIN2-associated ORE1 signalling pathways in Arabidopsis
Visit the PubMed ArticleNES1 expression indistinguishable in the ore1 mutant and the wild type
Du J, Li M, Kong D, Wang L, Lv Q, Wang J, Bao F, Gong Q, Xia J, He Y - Nitric oxide induces cotyledon senescence involving co-operation of the NES1/MAD1 and EIN2-associated ORE1 signalling pathways in Arabidopsis
Visit the PubMed Articlecue1 ein2, the level of ORE1 was low and not increased by treatment
Du J, Li M, Kong D, Wang L, Lv Q, Wang J, Bao F, Gong Q, Xia J, He Y - Nitric oxide induces cotyledon senescence involving co-operation of the NES1/MAD1 and EIN2-associated ORE1 signalling pathways in Arabidopsis
Visit the PubMed ArticleORE1 expression was indistinguishable in both the nes1-2 mutant and the wild type
Du J, Li M, Kong D, Wang L, Lv Q, Wang J, Bao F, Gong Q, Xia J, He Y - Nitric oxide induces cotyledon senescence involving co-operation of the NES1/MAD1 and EIN2-associated ORE1 signalling pathways in Arabidopsis
Visit the PubMed Articlein ore1, nitric oxide treatment changed the levels of SAG12 ... to a lesser extent than in the wild type
Du J, Li M, Kong D, Wang L, Lv Q, Wang J, Bao F, Gong Q, Xia J, He Y - Nitric oxide induces cotyledon senescence involving co-operation of the NES1/MAD1 and EIN2-associated ORE1 signalling pathways in Arabidopsis
Visit the PubMed Articlein ore1, nitric oxide treatment changed the levels of ... CAB2 to a lesser extent than in the wild type
Du J, Li M, Kong D, Wang L, Lv Q, Wang J, Bao F, Gong Q, Xia J, He Y - Nitric oxide induces cotyledon senescence involving co-operation of the NES1/MAD1 and EIN2-associated ORE1 signalling pathways in Arabidopsis
Visit the PubMed ArticleIn the absence of treatment ... transcripts were hardly detectable but ... with nitric oxide for 24h, the up-regulation of SAG12 ... was significantly stronger in nes1-2
Du J, Li M, Kong D, Wang L, Lv Q, Wang J, Bao F, Gong Q, Xia J, He Y - Nitric oxide induces cotyledon senescence involving co-operation of the NES1/MAD1 and EIN2-associated ORE1 signalling pathways in Arabidopsis
Visit the PubMed Articleto extend the analysis of expression to the protein level, a translational fusion was made between NES1 and GFP, driven by the NES1 promoter (pNES1::NES1::GFP). As a control, GFP alone driven by the NES1 promoter (pNES1::GFP) was used. Based on probing a western blot with anti-GFP antibody, the constructs were expressed in untreated seedlings
Du J, Li M, Kong D, Wang L, Lv Q, Wang J, Bao F, Gong Q, Xia J, He Y - Nitric oxide induces cotyledon senescence involving co-operation of the NES1/MAD1 and EIN2-associated ORE1 signalling pathways in Arabidopsis
Visit the PubMed Articlea translational fusion was made between NES1 and GFP, driven by the NES1 promoter (pNES1::NES1::GFP). As a control, GFP alone driven by the NES1 promoter (pNES1::GFP ... both were strongly up-regulated by treatment with nitroprusside, with no detectable signal in the wild-type control
Du J, Li M, Kong D, Wang L, Lv Q, Wang J, Bao F, Gong Q, Xia J, He Y - Nitric oxide induces cotyledon senescence involving co-operation of the NES1/MAD1 and EIN2-associated ORE1 signalling pathways in Arabidopsis
Visit the PubMed ArticleTo examine the expression of NES1, transgenic plants carrying a native NES1 promoter–GUS gene fusion were first prepared ... on the seventh day ... concentrated in the vasculature
Du J, Li M, Kong D, Wang L, Lv Q, Wang J, Bao F, Gong Q, Xia J, He Y - Nitric oxide induces cotyledon senescence involving co-operation of the NES1/MAD1 and EIN2-associated ORE1 signalling pathways in Arabidopsis
Visit the PubMed Articleein2 essentially had a limited background level of the ORE1 message in cotyledons. However this was not increased by nitric oxide treatment (Fig. 4G), meaning that the ORE1 induction relied on EIN2
Du J, Li M, Kong D, Wang L, Lv Q, Wang J, Bao F, Gong Q, Xia J, He Y - Nitric oxide induces cotyledon senescence involving co-operation of the NES1/MAD1 and EIN2-associated ORE1 signalling pathways in Arabidopsis
Visit the PubMed ArticleTo examine the expression of NES1, transgenic plants carrying a native NES1 promoter–GUS gene fusion were first prepared. On the first day after germination, GUS activity was mainly detected in cotyledons
Du J, Li M, Kong D, Wang L, Lv Q, Wang J, Bao F, Gong Q, Xia J, He Y - Nitric oxide induces cotyledon senescence involving co-operation of the NES1/MAD1 and EIN2-associated ORE1 signalling pathways in Arabidopsis
Visit the PubMed ArticleWhen 5-day-old cotyledons were bisected into apical and basal halves, the latter had a slightly higher level of NES1 basic expression. However, after a 24h treatment with 20 μM nitroprusside, the expression was raised to essentially the same level in both regions
Du J, Li M, Kong D, Wang L, Lv Q, Wang J, Bao F, Gong Q, Xia J, He Y - Nitric oxide induces cotyledon senescence involving co-operation of the NES1/MAD1 and EIN2-associated ORE1 signalling pathways in Arabidopsis
Visit the PubMed Articlein ore1, nitric oxide treatment changed the levels of ... SAG13 ... to a lesser extent than in the wild type
Du J, Li M, Kong D, Wang L, Lv Q, Wang J, Bao F, Gong Q, Xia J, He Y - Nitric oxide induces cotyledon senescence involving co-operation of the NES1/MAD1 and EIN2-associated ORE1 signalling pathways in Arabidopsis
Visit the PubMed ArticleIn the absence of treatment ... transcripts were hardly detectable but ... with nitric oxide for 24h, the up-regulation of ... SAG13 was significantly stronger in nes1-2
Du J, Li M, Kong D, Wang L, Lv Q, Wang J, Bao F, Gong Q, Xia J, He Y - Nitric oxide induces cotyledon senescence involving co-operation of the NES1/MAD1 and EIN2-associated ORE1 signalling pathways in Arabidopsis
Visit the PubMed ArticleTo examine the expression of NES1, transgenic plants carrying a native NES1 promoter–GUS gene fusion were first prepared ... on the seventh day, GUS was more widely expressed throughout the seedling
Du J, Li M, Kong D, Wang L, Lv Q, Wang J, Bao F, Gong Q, Xia J, He Y - Nitric oxide induces cotyledon senescence involving co-operation of the NES1/MAD1 and EIN2-associated ORE1 signalling pathways in Arabidopsis
Visit the PubMed ArticleTo examine the expression of NES1 further, quantitative real-time RT-PCR was used ... the relative transcript levels were slightly higher in the cotyledons
Du J, Li M, Kong D, Wang L, Lv Q, Wang J, Bao F, Gong Q, Xia J, He Y - Nitric oxide induces cotyledon senescence involving co-operation of the NES1/MAD1 and EIN2-associated ORE1 signalling pathways in Arabidopsis
Visit the PubMed ArticleIn the ein2 mutant, transcription of ORE1 declined significantly
Du J, Li M, Kong D, Wang L, Lv Q, Wang J, Bao F, Gong Q, Xia J, He Y - Nitric oxide induces cotyledon senescence involving co-operation of the NES1/MAD1 and EIN2-associated ORE1 signalling pathways in Arabidopsis
Visit the PubMed ArticleWe analyzed public transcriptome data and found that SHYG expression increased 4.3-fold in rosettes of fully submerged Arabidopsis plants within 7 h (Lee et al., 2011), indicating that it might act as a transcriptional regulator in waterlogging- or submergence-controlled processes
Rauf M, Arif M, Fisahn J, Xue GP, Balazadeh S, Mueller-Roeber B - NAC transcription factor speedy hyponastic growth regulates flooding-induced leaf movement in Arabidopsis
Visit the PubMed ArticleWe also tested SHYG expression by quantitative real-time PCR (qRT-PCR) and, like in the GUS studies, did not detect a major difference between the abaxial and adaxial petiole regions
Rauf M, Arif M, Fisahn J, Xue GP, Balazadeh S, Mueller-Roeber B - NAC transcription factor speedy hyponastic growth regulates flooding-induced leaf movement in Arabidopsis
Visit the PubMed ArticleExpression of ... ACO5 ... increased upon waterlogging in the proximal petiole segments of Col-0 but remained largely unaffected in Cvi-0, in accordance with its weak growth response
Rauf M, Arif M, Fisahn J, Xue GP, Balazadeh S, Mueller-Roeber B - NAC transcription factor speedy hyponastic growth regulates flooding-induced leaf movement in Arabidopsis
Visit the PubMed ArticleEXPA8 ... Upon root flooding ... in abaxial petiole cells ... remained low in shyg-2
Rauf M, Arif M, Fisahn J, Xue GP, Balazadeh S, Mueller-Roeber B - NAC transcription factor speedy hyponastic growth regulates flooding-induced leaf movement in Arabidopsis
Visit the PubMed ArticleUpon root flooding ... EXPA11 expression in abaxial petiole cells ... remained low in shyg-2
Rauf M, Arif M, Fisahn J, Xue GP, Balazadeh S, Mueller-Roeber B - NAC transcription factor speedy hyponastic growth regulates flooding-induced leaf movement in Arabidopsis
Visit the PubMed ArticleTo test whether enhanced SHYG expression is due to promoter activation, we tested waterlogging-dependent GUS activity in SHYG promoter-GUS fusion (ProSHYG:GUS) lines. GUS staining in petioles and the rosette center was weak in nonstressed plants. However, GUS activity was strongly elevated after 8 h of waterlogging (Figures 6A and 6B), when petiole angle was also increased in these lines (Figure 6A; see Supplemental Figure 7 online); this response was further enhanced by simultaneous application of ACC but diminished by AIB (Figures 6A and 6B; see Supplemental Figure 7 online), indicating a positive feedback loop connecting SHYG expression with ET level, most likely via ACO5
Rauf M, Arif M, Fisahn J, Xue GP, Balazadeh S, Mueller-Roeber B - NAC transcription factor speedy hyponastic growth regulates flooding-induced leaf movement in Arabidopsis
Visit the PubMed ArticleWe also tested SHYG expression in the proximal segment of the leaf petiole using the ProSHYG:GUS lines. As seen in Supplemental Figure 6A online, β-glucuronidase (GUS) staining was low before waterlogging but was strongly enhanced after 8 h of waterlogging. However, no major difference in staining intensity between the abaxial and adaxial petiole regions was observed
Rauf M, Arif M, Fisahn J, Xue GP, Balazadeh S, Mueller-Roeber B - NAC transcription factor speedy hyponastic growth regulates flooding-induced leaf movement in Arabidopsis
Visit the PubMed ArticleUpon root flooding ... in the 35S:SHYG overexpressor plants ... EXPA11 remained largely unaffected in adaxial cells
Rauf M, Arif M, Fisahn J, Xue GP, Balazadeh S, Mueller-Roeber B - NAC transcription factor speedy hyponastic growth regulates flooding-induced leaf movement in Arabidopsis
Visit the PubMed ArticleUpon root flooding ... in shyg-2 ... EXPA11 remained largely unaffected in adaxial cells
Rauf M, Arif M, Fisahn J, Xue GP, Balazadeh S, Mueller-Roeber B - NAC transcription factor speedy hyponastic growth regulates flooding-induced leaf movement in Arabidopsis
Visit the PubMed ArticleFirst, to identify genes downstream of SHYG, we expressed it from an estradiol-inducible promoter (Zuo et al., 2000) and performed expression profiling 5 h after β-estradiol treatment, using 14-d-old transgenic seedlings (SHYG-IOE). Only few genes robustly increased upon SHYG induction, including ACO5 (4.3-fold), encoding an uncharacterized enzyme involved in ET biosynthesis (see Supplemental Table 1 online). ACO5 transcript abundance and ACO protein level increased upon SHYG induction in a time-dependent manner (Figures 1A and 1B)
Rauf M, Arif M, Fisahn J, Xue GP, Balazadeh S, Mueller-Roeber B - NAC transcription factor speedy hyponastic growth regulates flooding-induced leaf movement in Arabidopsis
Visit the PubMed ArticleSHYG fused to green fluorescent protein (GFP) accumulated in nuclei of transformed Arabidopsis plants (see Supplemental Figure 2 online), consistent with its function as a TF
Rauf M, Arif M, Fisahn J, Xue GP, Balazadeh S, Mueller-Roeber B - NAC transcription factor speedy hyponastic growth regulates flooding-induced leaf movement in Arabidopsis
Visit the PubMed ArticleEXPA8 expression was considerably higher in the abaxial than adaxial petiole regions
Rauf M, Arif M, Fisahn J, Xue GP, Balazadeh S, Mueller-Roeber B - NAC transcription factor speedy hyponastic growth regulates flooding-induced leaf movement in Arabidopsis
Visit the PubMed ArticleEXPA8 ... Upon root flooding ... expression increased in shoot-proximal abaxial petiole segments of wild-type plants
Rauf M, Arif M, Fisahn J, Xue GP, Balazadeh S, Mueller-Roeber B - NAC transcription factor speedy hyponastic growth regulates flooding-induced leaf movement in Arabidopsis
Visit the PubMed ArticleWe ... did not detect a major difference between the abaxial and adaxial petiole regions ... ACO5
Rauf M, Arif M, Fisahn J, Xue GP, Balazadeh S, Mueller-Roeber B - NAC transcription factor speedy hyponastic growth regulates flooding-induced leaf movement in Arabidopsis
Visit the PubMed ArticleACO5 mRNA ... showed reduced levels in two homozygous T-DNA insertion lines, shyg-1 and shyg-2
Rauf M, Arif M, Fisahn J, Xue GP, Balazadeh S, Mueller-Roeber B - NAC transcription factor speedy hyponastic growth regulates flooding-induced leaf movement in Arabidopsis
Visit the PubMed ArticleEXPA8 ... Upon root flooding ... expression in abaxial petiole cells increased to higher levels in the 35S:SHYG overexpressor plants
Rauf M, Arif M, Fisahn J, Xue GP, Balazadeh S, Mueller-Roeber B - NAC transcription factor speedy hyponastic growth regulates flooding-induced leaf movement in Arabidopsis
Visit the PubMed ArticleEXPA11 ... expression was considerably higher in the abaxial than adaxial petiole regions
Rauf M, Arif M, Fisahn J, Xue GP, Balazadeh S, Mueller-Roeber B - NAC transcription factor speedy hyponastic growth regulates flooding-induced leaf movement in Arabidopsis
Visit the PubMed ArticleExpression of SHYG ... increased upon waterlogging in the proximal petiole segments of Col-0 but remained largely unaffected in Cvi-0, in accordance with its weak growth response
Rauf M, Arif M, Fisahn J, Xue GP, Balazadeh S, Mueller-Roeber B - NAC transcription factor speedy hyponastic growth regulates flooding-induced leaf movement in Arabidopsis
Visit the PubMed ArticleACO5 mRNA was also more abundant in Arabidopsis 35S:SHYG overexpressors than in Col-0 wild-type plants
Rauf M, Arif M, Fisahn J, Xue GP, Balazadeh S, Mueller-Roeber B - NAC transcription factor speedy hyponastic growth regulates flooding-induced leaf movement in Arabidopsis
Visit the PubMed ArticleUpon root flooding, EXPA11 expression increased in shoot-proximal abaxial petiole segments of wild-type plants, as expected
Rauf M, Arif M, Fisahn J, Xue GP, Balazadeh S, Mueller-Roeber B - NAC transcription factor speedy hyponastic growth regulates flooding-induced leaf movement in Arabidopsis
Visit the PubMed ArticleTo further test the SHYG-ACO5 regulatory cascade, we next performed transactivation assays using Arabidopsis mesophyll cell protoplasts cotransfected with a 35S:SHYG effector plasmid and a reporter construct (pACO5-fLUC) carrying a fusion between the ACO5 promoter (1.87 kb) and firefly luciferase (fLUC) as a reporter for transcriptional activation. As shown in Figure 1D, SHYG strongly transactivated ACO5 expression 8 and 16 h after transfection
Rauf M, Arif M, Fisahn J, Xue GP, Balazadeh S, Mueller-Roeber B - NAC transcription factor speedy hyponastic growth regulates flooding-induced leaf movement in Arabidopsis
Visit the PubMed ArticleUpon root flooding ... EXPA11 expression in abaxial petiole cells increased to higher levels in the 35S:SHYG overexpressor plants
Rauf M, Arif M, Fisahn J, Xue GP, Balazadeh S, Mueller-Roeber B - NAC transcription factor speedy hyponastic growth regulates flooding-induced leaf movement in Arabidopsis
Visit the PubMed ArticleExpression of ... EXPA8 ... increased upon waterlogging in the proximal petiole segments of Col-0 but remained largely unaffected in Cvi-0, in accordance with its weak growth response
Rauf M, Arif M, Fisahn J, Xue GP, Balazadeh S, Mueller-Roeber B - NAC transcription factor speedy hyponastic growth regulates flooding-induced leaf movement in Arabidopsis
Visit the PubMed ArticlePR5 expression ... reduced in pad4 paps1 double mutants compared with paps1 plants
Trost G, Vi SL, Czesnick H, Lange P, Holton N, Giavalisco P, Zipfel C, Kappel C, Lenhard M - Arabidopsis poly(A) polymerase PAPS1 limits founder-cell recruitment to organ primordia and suppresses the salicylic acid-independent immune response downstream of EDS1/PAD4
Visit the PubMed ArticlePR2 ... expression ... reduced in pad4 paps1 double mutants compared with paps1 plants
Trost G, Vi SL, Czesnick H, Lange P, Holton N, Giavalisco P, Zipfel C, Kappel C, Lenhard M - Arabidopsis poly(A) polymerase PAPS1 limits founder-cell recruitment to organ primordia and suppresses the salicylic acid-independent immune response downstream of EDS1/PAD4
Visit the PubMed Articlein pad4 paps1 double mutants ... for SID2 the suppression ... was not significant
Trost G, Vi SL, Czesnick H, Lange P, Holton N, Giavalisco P, Zipfel C, Kappel C, Lenhard M - Arabidopsis poly(A) polymerase PAPS1 limits founder-cell recruitment to organ primordia and suppresses the salicylic acid-independent immune response downstream of EDS1/PAD4
Visit the PubMed Articlein paps1-1 mutants expressing either the PAPS4 coding sequence or a fusion of the N-terminal domain of PAPS4 and the C-terminal domain of PAPS1 under the control of the pPAPS1 promoter ... PR1 ... still overexpressed in pPAPS1::PAPS4 expressing paps1-1 mutants compared with wild-type ... but their expression was rescued to wild-type levels in the pPAPS1::PAPS4N-PAPS1C expressing paps1-1 mutants
Trost G, Vi SL, Czesnick H, Lange P, Holton N, Giavalisco P, Zipfel C, Kappel C, Lenhard M - Arabidopsis poly(A) polymerase PAPS1 limits founder-cell recruitment to organ primordia and suppresses the salicylic acid-independent immune response downstream of EDS1/PAD4
Visit the PubMed ArticlePR1 ... expression ... reduced in pad4 paps1 double mutants compared with paps1 plants
Trost G, Vi SL, Czesnick H, Lange P, Holton N, Giavalisco P, Zipfel C, Kappel C, Lenhard M - Arabidopsis poly(A) polymerase PAPS1 limits founder-cell recruitment to organ primordia and suppresses the salicylic acid-independent immune response downstream of EDS1/PAD4
Visit the PubMed Articlein paps1-1 mutants expressing either the PAPS4 coding sequence or a fusion of the N-terminal domain of PAPS4 and the C-terminal domain of PAPS1 under the control of the pPAPS1 promoter ... PR2 ... still overexpressed in pPAPS1::PAPS4 expressing paps1-1 mutants compared with wild-type, but their expression was rescued to wild-type levels in the pPAPS1::PAPS4N-PAPS1C expressing paps1-1 mutants
Trost G, Vi SL, Czesnick H, Lange P, Holton N, Giavalisco P, Zipfel C, Kappel C, Lenhard M - Arabidopsis poly(A) polymerase PAPS1 limits founder-cell recruitment to organ primordia and suppresses the salicylic acid-independent immune response downstream of EDS1/PAD4
Visit the PubMed ArticleGenevestigator toolbox ... In response to DNA stress (genotoxic stress and UV-B light treatment), two SMR genes responded strongly, namely ... SMR5
Yi D, Alvim Kamei CL, Cools T, Vanderauwera S, Takahashi N, Okushima Y, Eekhout T, Yoshiyama KO, Larkin J, Van den Daele H, Conklin P, Britt A, Umeda M, De Veylder L - The Arabidopsis SIAMESE-RELATED cyclin-dependent kinase inhibitors SMR5 and SMR7 regulate the DNA damage checkpoint in response to reactive oxygen species
Visit the PubMed ArticleGenevestigator toolbox ... In response to DNA stress (genotoxic stress and UV-B light treatment), two SMR genes responded strongly, namely, SMR4
Yi D, Alvim Kamei CL, Cools T, Vanderauwera S, Takahashi N, Okushima Y, Eekhout T, Yoshiyama KO, Larkin J, Van den Daele H, Conklin P, Britt A, Umeda M, De Veylder L - The Arabidopsis SIAMESE-RELATED cyclin-dependent kinase inhibitors SMR5 and SMR7 regulate the DNA damage checkpoint in response to reactive oxygen species
Visit the PubMed ArticleGenevestigator toolbox ... SMR5 responding to most diverse types of abiotic stresses
Yi D, Alvim Kamei CL, Cools T, Vanderauwera S, Takahashi N, Okushima Y, Eekhout T, Yoshiyama KO, Larkin J, Van den Daele H, Conklin P, Britt A, Umeda M, De Veylder L - The Arabidopsis SIAMESE-RELATED cyclin-dependent kinase inhibitors SMR5 and SMR7 regulate the DNA damage checkpoint in response to reactive oxygen species
Visit the PubMed ArticleIn addition to HU and bleomycin, we confirmed the transcriptional activation of ... SMR7 by γ-irradiation
Yi D, Alvim Kamei CL, Cools T, Vanderauwera S, Takahashi N, Okushima Y, Eekhout T, Yoshiyama KO, Larkin J, Van den Daele H, Conklin P, Britt A, Umeda M, De Veylder L - The Arabidopsis SIAMESE-RELATED cyclin-dependent kinase inhibitors SMR5 and SMR7 regulate the DNA damage checkpoint in response to reactive oxygen species
Visit the PubMed ArticleSMR5 ... induced in the proliferating leaf upon HU and bleomycin treatment
Yi D, Alvim Kamei CL, Cools T, Vanderauwera S, Takahashi N, Okushima Y, Eekhout T, Yoshiyama KO, Larkin J, Van den Daele H, Conklin P, Britt A, Umeda M, De Veylder L - The Arabidopsis SIAMESE-RELATED cyclin-dependent kinase inhibitors SMR5 and SMR7 regulate the DNA damage checkpoint in response to reactive oxygen species
Visit the PubMed ArticleTo identify novel key signaling components that contribute to cell cycle checkpoint activation, we compared bleomycin-induced genes to those induced by HU treatment (Cools et al., 2010) and γ-radiation (Culligan et al., 2006; Yoshiyama et al., 2009). Twenty-two genes were upregulated in all DNA stress experiments and can be considered as transcriptional hallmarks of the DNA damage response, regardless of the type of DNA stress ... including ... RAS-ASSOCIATED WITH DIABETES PROTEIN51
Yi D, Alvim Kamei CL, Cools T, Vanderauwera S, Takahashi N, Okushima Y, Eekhout T, Yoshiyama KO, Larkin J, Van den Daele H, Conklin P, Britt A, Umeda M, De Veylder L - The Arabidopsis SIAMESE-RELATED cyclin-dependent kinase inhibitors SMR5 and SMR7 regulate the DNA damage checkpoint in response to reactive oxygen species
Visit the PubMed ArticleSMR5 transcript levels appeared to be stable ... in the ... apx1 cat2 double mutant
Yi D, Alvim Kamei CL, Cools T, Vanderauwera S, Takahashi N, Okushima Y, Eekhout T, Yoshiyama KO, Larkin J, Van den Daele H, Conklin P, Britt A, Umeda M, De Veylder L - The Arabidopsis SIAMESE-RELATED cyclin-dependent kinase inhibitors SMR5 and SMR7 regulate the DNA damage checkpoint in response to reactive oxygen species
Visit the PubMed ArticleTo identify novel key signaling components that contribute to cell cycle checkpoint activation, we compared bleomycin-induced genes to those induced by HU treatment (Cools et al., 2010) and γ-radiation (Culligan et al., 2006; Yoshiyama et al., 2009). Twenty-two genes were upregulated in all DNA stress experiments and can be considered as transcriptional hallmarks of the DNA damage response, regardless of the type of DNA stress ... including ... one member of the SIM/SMR gene family, SMR5
Yi D, Alvim Kamei CL, Cools T, Vanderauwera S, Takahashi N, Okushima Y, Eekhout T, Yoshiyama KO, Larkin J, Van den Daele H, Conklin P, Britt A, Umeda M, De Veylder L - The Arabidopsis SIAMESE-RELATED cyclin-dependent kinase inhibitors SMR5 and SMR7 regulate the DNA damage checkpoint in response to reactive oxygen species
Visit the PubMed ArticleSMR5 induction under high light was confirmed by RT-PCR
Yi D, Alvim Kamei CL, Cools T, Vanderauwera S, Takahashi N, Okushima Y, Eekhout T, Yoshiyama KO, Larkin J, Van den Daele H, Conklin P, Britt A, Umeda M, De Veylder L - The Arabidopsis SIAMESE-RELATED cyclin-dependent kinase inhibitors SMR5 and SMR7 regulate the DNA damage checkpoint in response to reactive oxygen species
Visit the PubMed Articleintroducing the SMR5 ... ß-glucuronidase (GUS) reporter lines into ... sog1-1 mutant ... the transcriptional activation of SMR5 ... by bleomycin depended on ... SOG1 ... the same pattern was observed for HU
Yi D, Alvim Kamei CL, Cools T, Vanderauwera S, Takahashi N, Okushima Y, Eekhout T, Yoshiyama KO, Larkin J, Van den Daele H, Conklin P, Britt A, Umeda M, De Veylder L - The Arabidopsis SIAMESE-RELATED cyclin-dependent kinase inhibitors SMR5 and SMR7 regulate the DNA damage checkpoint in response to reactive oxygen species
Visit the PubMed ArticleTo identify novel key signaling components that contribute to cell cycle checkpoint activation, we compared bleomycin-induced genes to those induced by HU treatment (Cools et al., 2010) and γ-radiation (Culligan et al., 2006; Yoshiyama et al., 2009). Twenty-two genes were upregulated in all DNA stress experiments and can be considered as transcriptional hallmarks of the DNA damage response, regardless of the type of DNA stress ... including POLY(ADP-RIBOSE) POLYMERASE2 (PARP2)
Yi D, Alvim Kamei CL, Cools T, Vanderauwera S, Takahashi N, Okushima Y, Eekhout T, Yoshiyama KO, Larkin J, Van den Daele H, Conklin P, Britt A, Umeda M, De Veylder L - The Arabidopsis SIAMESE-RELATED cyclin-dependent kinase inhibitors SMR5 and SMR7 regulate the DNA damage checkpoint in response to reactive oxygen species
Visit the PubMed ArticleIn addition to HU and bleomycin, we confirmed the transcriptional activation of ... SMR5 ... by γ-irradiation
Yi D, Alvim Kamei CL, Cools T, Vanderauwera S, Takahashi N, Okushima Y, Eekhout T, Yoshiyama KO, Larkin J, Van den Daele H, Conklin P, Britt A, Umeda M, De Veylder L - The Arabidopsis SIAMESE-RELATED cyclin-dependent kinase inhibitors SMR5 and SMR7 regulate the DNA damage checkpoint in response to reactive oxygen species
Visit the PubMed ArticleIn addition to HU and bleomycin, we confirmed the transcriptional activation of SMR4 ... by γ-irradiation
Yi D, Alvim Kamei CL, Cools T, Vanderauwera S, Takahashi N, Okushima Y, Eekhout T, Yoshiyama KO, Larkin J, Van den Daele H, Conklin P, Britt A, Umeda M, De Veylder L - The Arabidopsis SIAMESE-RELATED cyclin-dependent kinase inhibitors SMR5 and SMR7 regulate the DNA damage checkpoint in response to reactive oxygen species
Visit the PubMed ArticleAs an independent strategy to induce ROS, SMR5 ... GUS reporter lines were transferred from control (70 to 80 µmol m–2 s–1) to high light (300 to 400 µmol m–2 s–1) conditions ... SMR5 promoter activity was strongly stimulated in both the shoot apex and leaf tissue
Yi D, Alvim Kamei CL, Cools T, Vanderauwera S, Takahashi N, Okushima Y, Eekhout T, Yoshiyama KO, Larkin J, Van den Daele H, Conklin P, Britt A, Umeda M, De Veylder L - The Arabidopsis SIAMESE-RELATED cyclin-dependent kinase inhibitors SMR5 and SMR7 regulate the DNA damage checkpoint in response to reactive oxygen species
Visit the PubMed ArticleAs an independent strategy to induce ROS ... SMR7 GUS reporter lines were transferred from control (70 to 80 µmol m–2 s–1) to high light (300 to 400 µmol m–2 s–1) conditions for 2 d ... PSMR7:GUS plants displayed an increase in GUS activity being mainly restricted to the shoot apex
Yi D, Alvim Kamei CL, Cools T, Vanderauwera S, Takahashi N, Okushima Y, Eekhout T, Yoshiyama KO, Larkin J, Van den Daele H, Conklin P, Britt A, Umeda M, De Veylder L - The Arabidopsis SIAMESE-RELATED cyclin-dependent kinase inhibitors SMR5 and SMR7 regulate the DNA damage checkpoint in response to reactive oxygen species
Visit the PubMed ArticleSMR5 transcript levels appeared to be stable ... in the single apx1 ... mutant
Yi D, Alvim Kamei CL, Cools T, Vanderauwera S, Takahashi N, Okushima Y, Eekhout T, Yoshiyama KO, Larkin J, Van den Daele H, Conklin P, Britt A, Umeda M, De Veylder L - The Arabidopsis SIAMESE-RELATED cyclin-dependent kinase inhibitors SMR5 and SMR7 regulate the DNA damage checkpoint in response to reactive oxygen species
Visit the PubMed Articleintroducing the ... SMR7 ß-glucuronidase (GUS) reporter lines into the ... sog1-1 mutant ... the transcriptional activation of ... SMR7 by bleomycin depended on ... SOG1 ... the same pattern was observed for HU
Yi D, Alvim Kamei CL, Cools T, Vanderauwera S, Takahashi N, Okushima Y, Eekhout T, Yoshiyama KO, Larkin J, Van den Daele H, Conklin P, Britt A, Umeda M, De Veylder L - The Arabidopsis SIAMESE-RELATED cyclin-dependent kinase inhibitors SMR5 and SMR7 regulate the DNA damage checkpoint in response to reactive oxygen species
Visit the PubMed ArticleSMR7 ... induced in the proliferating leaf upon HU and bleomycin treatment
Yi D, Alvim Kamei CL, Cools T, Vanderauwera S, Takahashi N, Okushima Y, Eekhout T, Yoshiyama KO, Larkin J, Van den Daele H, Conklin P, Britt A, Umeda M, De Veylder L - The Arabidopsis SIAMESE-RELATED cyclin-dependent kinase inhibitors SMR5 and SMR7 regulate the DNA damage checkpoint in response to reactive oxygen species
Visit the PubMed Articleintroducing the SMR5 ... ß-glucuronidase (GUS) reporter lines into the atr-2, atm-1 ... the transcriptional activation of SMR5 ... by bleomycin depended on ATM ... the same pattern was observed for HU
Yi D, Alvim Kamei CL, Cools T, Vanderauwera S, Takahashi N, Okushima Y, Eekhout T, Yoshiyama KO, Larkin J, Van den Daele H, Conklin P, Britt A, Umeda M, De Veylder L - The Arabidopsis SIAMESE-RELATED cyclin-dependent kinase inhibitors SMR5 and SMR7 regulate the DNA damage checkpoint in response to reactive oxygen species
Visit the PubMed ArticleSMR7 expression levels were clearly induced in the single apx1 ... mutant
Yi D, Alvim Kamei CL, Cools T, Vanderauwera S, Takahashi N, Okushima Y, Eekhout T, Yoshiyama KO, Larkin J, Van den Daele H, Conklin P, Britt A, Umeda M, De Veylder L - The Arabidopsis SIAMESE-RELATED cyclin-dependent kinase inhibitors SMR5 and SMR7 regulate the DNA damage checkpoint in response to reactive oxygen species
Visit the PubMed ArticleTo identify novel key signaling components that contribute to cell cycle checkpoint activation, we compared bleomycin-induced genes to those induced by HU treatment (Cools et al., 2010) and γ-radiation (Culligan et al., 2006; Yoshiyama et al., 2009). Twenty-two genes were upregulated in all DNA stress experiments and can be considered as transcriptional hallmarks of the DNA damage response, regardless of the type of DNA stress ... including ... BREAST CANCER SUSCEPTIBILITY1 (BRCA1)
Yi D, Alvim Kamei CL, Cools T, Vanderauwera S, Takahashi N, Okushima Y, Eekhout T, Yoshiyama KO, Larkin J, Van den Daele H, Conklin P, Britt A, Umeda M, De Veylder L - The Arabidopsis SIAMESE-RELATED cyclin-dependent kinase inhibitors SMR5 and SMR7 regulate the DNA damage checkpoint in response to reactive oxygen species
Visit the PubMed Articleintroducing the ... SMR7 ß-glucuronidase (GUS) reporter lines into the atr-2, atm-1 ... the transcriptional activation of ... SMR7 by bleomycin depended on ATM ... the same pattern was observed for HU
Yi D, Alvim Kamei CL, Cools T, Vanderauwera S, Takahashi N, Okushima Y, Eekhout T, Yoshiyama KO, Larkin J, Van den Daele H, Conklin P, Britt A, Umeda M, De Veylder L - The Arabidopsis SIAMESE-RELATED cyclin-dependent kinase inhibitors SMR5 and SMR7 regulate the DNA damage checkpoint in response to reactive oxygen species
Visit the PubMed ArticleSMR7 expression levels were clearly induced in the ... apx1 cat2 double mutant
Yi D, Alvim Kamei CL, Cools T, Vanderauwera S, Takahashi N, Okushima Y, Eekhout T, Yoshiyama KO, Larkin J, Van den Daele H, Conklin P, Britt A, Umeda M, De Veylder L - The Arabidopsis SIAMESE-RELATED cyclin-dependent kinase inhibitors SMR5 and SMR7 regulate the DNA damage checkpoint in response to reactive oxygen species
Visit the PubMed Articlethe transcript levels of SEN4 ... in the wrky57-1 mutant were not affected by treatment with MeJA+IAA compared with MeJA
Jiang Y, Liang G, Yang S, Yu D - Arabidopsis WRKY57 functions as a node of convergence for jasmonic acid- and auxin-mediated signaling in jasmonic acid-induced leaf senescence
Visit the PubMed Articlethe transcript levels of ... JAZ8 were higher in senescent leaves than in normal leaves
Jiang Y, Liang G, Yang S, Yu D - Arabidopsis WRKY57 functions as a node of convergence for jasmonic acid- and auxin-mediated signaling in jasmonic acid-induced leaf senescence
Visit the PubMed ArticleSimilarly to the wild type, WRKY57 transcript levels in ProWRKY57:Myc-WRKY57 were upregulated after MeJA treatment (Supplemental Figure 5). By contrast, WRKY57 protein levels decreased with the treatment of MeJA
Jiang Y, Liang G, Yang S, Yu D - Arabidopsis WRKY57 functions as a node of convergence for jasmonic acid- and auxin-mediated signaling in jasmonic acid-induced leaf senescence
Visit the PubMed ArticleJAZ4 ... overexpression plants ... SAG12 ... downregulated
Jiang Y, Liang G, Yang S, Yu D - Arabidopsis WRKY57 functions as a node of convergence for jasmonic acid- and auxin-mediated signaling in jasmonic acid-induced leaf senescence
Visit the PubMed ArticleThe degradation of WRKY57 was clearly attenuated by the 26S proteasome inhibitor MG132
Jiang Y, Liang G, Yang S, Yu D - Arabidopsis WRKY57 functions as a node of convergence for jasmonic acid- and auxin-mediated signaling in jasmonic acid-induced leaf senescence
Visit the PubMed ArticleSAG18 ... After treatment with MeJA ... transcript levels were upregulated significantly in the wrky57 mutants compared with the wild type and the overexpression line
Jiang Y, Liang G, Yang S, Yu D - Arabidopsis WRKY57 functions as a node of convergence for jasmonic acid- and auxin-mediated signaling in jasmonic acid-induced leaf senescence
Visit the PubMed Articlethe transcript levels of JAZ4 ... were higher in senescent leaves than in normal leaves
Jiang Y, Liang G, Yang S, Yu D - Arabidopsis WRKY57 functions as a node of convergence for jasmonic acid- and auxin-mediated signaling in jasmonic acid-induced leaf senescence
Visit the PubMed ArticleWe first examined the transcript levels of WRKY57 in response to auxin treatment and found that WRKY57 was dramatically induced by auxin treatment
Jiang Y, Liang G, Yang S, Yu D - Arabidopsis WRKY57 functions as a node of convergence for jasmonic acid- and auxin-mediated signaling in jasmonic acid-induced leaf senescence
Visit the PubMed ArticleIAA29 ... WRKY57 and its partners colocalize ... in plant cell nuclei
Jiang Y, Liang G, Yang S, Yu D - Arabidopsis WRKY57 functions as a node of convergence for jasmonic acid- and auxin-mediated signaling in jasmonic acid-induced leaf senescence
Visit the PubMed ArticleSAG12 ... After treatment with MeJA ... transcript levels were upregulated significantly in the wrky57 mutants compared with the wild type and the overexpression line
Jiang Y, Liang G, Yang S, Yu D - Arabidopsis WRKY57 functions as a node of convergence for jasmonic acid- and auxin-mediated signaling in jasmonic acid-induced leaf senescence
Visit the PubMed ArticleSEN4 ... After treatment with MeJA ... transcript levels were upregulated significantly in the wrky57 mutants compared with the wild type and the overexpression line
Jiang Y, Liang G, Yang S, Yu D - Arabidopsis WRKY57 functions as a node of convergence for jasmonic acid- and auxin-mediated signaling in jasmonic acid-induced leaf senescence
Visit the PubMed Articlethe transcript levels of SEN4 ... were higher in IAA29 overexpression plants than in the wild type
Jiang Y, Liang G, Yang S, Yu D - Arabidopsis WRKY57 functions as a node of convergence for jasmonic acid- and auxin-mediated signaling in jasmonic acid-induced leaf senescence
Visit the PubMed ArticleJAZ8 overexpression plants ... SAG12 ... downregulated
Jiang Y, Liang G, Yang S, Yu D - Arabidopsis WRKY57 functions as a node of convergence for jasmonic acid- and auxin-mediated signaling in jasmonic acid-induced leaf senescence
Visit the PubMed Articlethe transcript levels of ... SAG12 were significantly downregulated in leaves treated with MeJA+IAA compared with those treated with MeJA
Jiang Y, Liang G, Yang S, Yu D - Arabidopsis WRKY57 functions as a node of convergence for jasmonic acid- and auxin-mediated signaling in jasmonic acid-induced leaf senescence
Visit the PubMed ArticleJAZ4 ... WRKY57 and its partners colocalize ... in plant cell nuclei
Jiang Y, Liang G, Yang S, Yu D - Arabidopsis WRKY57 functions as a node of convergence for jasmonic acid- and auxin-mediated signaling in jasmonic acid-induced leaf senescence
Visit the PubMed ArticleAlthough WRKY57 transcripts are induced by JA, WRKY57 functions as a negative regulator in JA-induced senescence. However, because mRNA levels sometimes do not reflect protein levels, it was necessary to determine the protein levels of WRKY57. For this purpose, WRKY57 cDNA fused with a Myc tag and driven by a 2.5-kb WRKY57 promoter was transformed into the wrky57-1 mutant. The expression of the Myc tag was used to monitor the protein levels of WRKY57 (by protein gel blot analysis). As shown in Figure 8A, WRKY57 protein levels in ProWRKY57: Myc-WRKY57 were not induced by water (mock) (Figure 8A). We then checked the response of WRKY57 to auxin at both the mRNA and protein levels in ProWRKY57:Myc-WRKY57, revealing that either mRNA or protein levels of WRKY57 were induced by auxin treatment
Jiang Y, Liang G, Yang S, Yu D - Arabidopsis WRKY57 functions as a node of convergence for jasmonic acid- and auxin-mediated signaling in jasmonic acid-induced leaf senescence
Visit the PubMed ArticleJAZ4 ... overexpression plants ... SEN4 ... downregulated
Jiang Y, Liang G, Yang S, Yu D - Arabidopsis WRKY57 functions as a node of convergence for jasmonic acid- and auxin-mediated signaling in jasmonic acid-induced leaf senescence
Visit the PubMed ArticleSEN4 ... upregulated in the ... jaz8
Jiang Y, Liang G, Yang S, Yu D - Arabidopsis WRKY57 functions as a node of convergence for jasmonic acid- and auxin-mediated signaling in jasmonic acid-induced leaf senescence
Visit the PubMed ArticleSAG20 ... After treatment with MeJA ... transcript levels were upregulated significantly in the wrky57 mutants compared with the wild type and the overexpression line
Jiang Y, Liang G, Yang S, Yu D - Arabidopsis WRKY57 functions as a node of convergence for jasmonic acid- and auxin-mediated signaling in jasmonic acid-induced leaf senescence
Visit the PubMed Articletranscript levels of IAA29 ... were higher in senescent leaves than in normal leaves
Jiang Y, Liang G, Yang S, Yu D - Arabidopsis WRKY57 functions as a node of convergence for jasmonic acid- and auxin-mediated signaling in jasmonic acid-induced leaf senescence
Visit the PubMed Articlethe transcript levels of SEN4 ... were significantly downregulated in leaves treated with MeJA+IAA compared with those treated with MeJA
Jiang Y, Liang G, Yang S, Yu D - Arabidopsis WRKY57 functions as a node of convergence for jasmonic acid- and auxin-mediated signaling in jasmonic acid-induced leaf senescence
Visit the PubMed Articlethe transcript levels of ... SAG12 in the wrky57-1 mutant were not affected by treatment with MeJA+IAA compared with MeJA
Jiang Y, Liang G, Yang S, Yu D - Arabidopsis WRKY57 functions as a node of convergence for jasmonic acid- and auxin-mediated signaling in jasmonic acid-induced leaf senescence
Visit the PubMed Articlethe transcript levels of ... SAG12 were higher in IAA29 overexpression plants than in the wild type
Jiang Y, Liang G, Yang S, Yu D - Arabidopsis WRKY57 functions as a node of convergence for jasmonic acid- and auxin-mediated signaling in jasmonic acid-induced leaf senescence
Visit the PubMed ArticleJAZ8 ... WRKY57 and its partners colocalize ... in plant cell nuclei
Jiang Y, Liang G, Yang S, Yu D - Arabidopsis WRKY57 functions as a node of convergence for jasmonic acid- and auxin-mediated signaling in jasmonic acid-induced leaf senescence
Visit the PubMed ArticleWRKY57 and its partners colocalize ... in plant cell nuclei
Jiang Y, Liang G, Yang S, Yu D - Arabidopsis WRKY57 functions as a node of convergence for jasmonic acid- and auxin-mediated signaling in jasmonic acid-induced leaf senescence
Visit the PubMed ArticleJAZ8 overexpression plants ... SEN4 ... downregulated
Jiang Y, Liang G, Yang S, Yu D - Arabidopsis WRKY57 functions as a node of convergence for jasmonic acid- and auxin-mediated signaling in jasmonic acid-induced leaf senescence
Visit the PubMed ArticleSAG12 ... upregulated in the jaz4
Jiang Y, Liang G, Yang S, Yu D - Arabidopsis WRKY57 functions as a node of convergence for jasmonic acid- and auxin-mediated signaling in jasmonic acid-induced leaf senescence
Visit the PubMed ArticleSEN4 ... upregulated in the jaz4
Jiang Y, Liang G, Yang S, Yu D - Arabidopsis WRKY57 functions as a node of convergence for jasmonic acid- and auxin-mediated signaling in jasmonic acid-induced leaf senescence
Visit the PubMed ArticleWe also determined the protein levels of WRKY57 in coi1 mutants, and the results demonstrated that the protein degradation is partially dependent on COI1
Jiang Y, Liang G, Yang S, Yu D - Arabidopsis WRKY57 functions as a node of convergence for jasmonic acid- and auxin-mediated signaling in jasmonic acid-induced leaf senescence
Visit the PubMed ArticleTo determine whether WRKY57 transcription is associated with phytohormones, we checked the transcript levels of WRKY57 in response to exogenous phytohormone treatments, revealing that JA significantly induces WRKY57
Jiang Y, Liang G, Yang S, Yu D - Arabidopsis WRKY57 functions as a node of convergence for jasmonic acid- and auxin-mediated signaling in jasmonic acid-induced leaf senescence
Visit the PubMed Articledeveloping first leaves of AN3-GR and wild-type plants were subjected to transcript profiling using Affymetrix ATH1 microarrays after transfer at 8 DAS to DEX-containing medium for 8 h ... four members of the GRF family were upregulated ... GRF6
Vercruyssen L, Verkest A, Gonzalez N, Heyndrickx KS, Eeckhout D, Han SK, Jégu T, Archacki R, Van Leene J, Andriankaja M, De Bodt S, Abeel T, Coppens F, Dhondt S, De Milde L, Vermeersch M, Maleux K, Gevaert K, Jerzmanowski A, Benhamed M, Wagner D, Vandepoele K, De Jaeger G, Inzé D. - ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development
Visit the PubMed ArticleGRF5 ... expression levels were downregulated in 12-d-old an3 rosettes
Vercruyssen L, Verkest A, Gonzalez N, Heyndrickx KS, Eeckhout D, Han SK, Jégu T, Archacki R, Van Leene J, Andriankaja M, De Bodt S, Abeel T, Coppens F, Dhondt S, De Milde L, Vermeersch M, Maleux K, Gevaert K, Jerzmanowski A, Benhamed M, Wagner D, Vandepoele K, De Jaeger G, Inzé D. - ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development
Visit the PubMed ArticleGRF6 expression levels were downregulated in 12-d-old an3 rosettes
Vercruyssen L, Verkest A, Gonzalez N, Heyndrickx KS, Eeckhout D, Han SK, Jégu T, Archacki R, Van Leene J, Andriankaja M, De Bodt S, Abeel T, Coppens F, Dhondt S, De Milde L, Vermeersch M, Maleux K, Gevaert K, Jerzmanowski A, Benhamed M, Wagner D, Vandepoele K, De Jaeger G, Inzé D. - ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development
Visit the PubMed ArticleTo analyze this in more detail, a time-course experiment was conducted, in which RNA levels were quantified with quantitative RT-PCR (qRT-PCR) at 1, 2, 4, and 6 h after transfer of AN3-GR and wild-type plants to DEX-containing medium ... GRF6 ... induced significantly from 2 h onwards
Vercruyssen L, Verkest A, Gonzalez N, Heyndrickx KS, Eeckhout D, Han SK, Jégu T, Archacki R, Van Leene J, Andriankaja M, De Bodt S, Abeel T, Coppens F, Dhondt S, De Milde L, Vermeersch M, Maleux K, Gevaert K, Jerzmanowski A, Benhamed M, Wagner D, Vandepoele K, De Jaeger G, Inzé D. - ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development
Visit the PubMed ArticleqRT-PCR expression levels of ... GRF5 ... were found to be significantly reduced in 8-d-old brm3 shoots grown in long-day conditions compared with wild-type seedlings
Vercruyssen L, Verkest A, Gonzalez N, Heyndrickx KS, Eeckhout D, Han SK, Jégu T, Archacki R, Van Leene J, Andriankaja M, De Bodt S, Abeel T, Coppens F, Dhondt S, De Milde L, Vermeersch M, Maleux K, Gevaert K, Jerzmanowski A, Benhamed M, Wagner D, Vandepoele K, De Jaeger G, Inzé D. - ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development
Visit the PubMed ArticleTo analyze this in more detail, a time-course experiment was conducted, in which RNA levels were quantified with quantitative RT-PCR (qRT-PCR) at 1, 2, 4, and 6 h after transfer of AN3-GR and wild-type plants to DEX-containing medium ... transiently significantly upregulated ... after transfer to DEX ... CONSTANS-LIKE5 (COL5) after 4 and 6 h
Vercruyssen L, Verkest A, Gonzalez N, Heyndrickx KS, Eeckhout D, Han SK, Jégu T, Archacki R, Van Leene J, Andriankaja M, De Bodt S, Abeel T, Coppens F, Dhondt S, De Milde L, Vermeersch M, Maleux K, Gevaert K, Jerzmanowski A, Benhamed M, Wagner D, Vandepoele K, De Jaeger G, Inzé D. - ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development
Visit the PubMed ArticleTo analyze this in more detail, a time-course experiment was conducted, in which RNA levels were quantified with quantitative RT-PCR (qRT-PCR) at 1, 2, 4, and 6 h after transfer of AN3-GR and wild-type plants to DEX-containing medium ... GRF3 ... induced significantly from 2 h onwards
Vercruyssen L, Verkest A, Gonzalez N, Heyndrickx KS, Eeckhout D, Han SK, Jégu T, Archacki R, Van Leene J, Andriankaja M, De Bodt S, Abeel T, Coppens F, Dhondt S, De Milde L, Vermeersch M, Maleux K, Gevaert K, Jerzmanowski A, Benhamed M, Wagner D, Vandepoele K, De Jaeger G, Inzé D. - ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development
Visit the PubMed Articledeveloping first leaves of AN3-GR and wild-type plants were subjected to transcript profiling using Affymetrix ATH1 microarrays after transfer at 8 DAS to DEX-containing medium for 8 h ... four members of the GRF family were upregulated ... GRF5
Vercruyssen L, Verkest A, Gonzalez N, Heyndrickx KS, Eeckhout D, Han SK, Jégu T, Archacki R, Van Leene J, Andriankaja M, De Bodt S, Abeel T, Coppens F, Dhondt S, De Milde L, Vermeersch M, Maleux K, Gevaert K, Jerzmanowski A, Benhamed M, Wagner D, Vandepoele K, De Jaeger G, Inzé D. - ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development
Visit the PubMed ArticleTranscription factors with higher expression in ... 35S:GRF5 leaves encoded the basic helix-loop-helix transcriptional regulator HECATE1 (HEC1)
Vercruyssen L, Verkest A, Gonzalez N, Heyndrickx KS, Eeckhout D, Han SK, Jégu T, Archacki R, Van Leene J, Andriankaja M, De Bodt S, Abeel T, Coppens F, Dhondt S, De Milde L, Vermeersch M, Maleux K, Gevaert K, Jerzmanowski A, Benhamed M, Wagner D, Vandepoele K, De Jaeger G, Inzé D. - ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development
Visit the PubMed ArticleCOL5 ... downregulated in brm1 shoots
Vercruyssen L, Verkest A, Gonzalez N, Heyndrickx KS, Eeckhout D, Han SK, Jégu T, Archacki R, Van Leene J, Andriankaja M, De Bodt S, Abeel T, Coppens F, Dhondt S, De Milde L, Vermeersch M, Maleux K, Gevaert K, Jerzmanowski A, Benhamed M, Wagner D, Vandepoele K, De Jaeger G, Inzé D. - ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development
Visit the PubMed ArticleARR4 ... was found to be ... downregulated in 35S:GRF5 plants
Vercruyssen L, Verkest A, Gonzalez N, Heyndrickx KS, Eeckhout D, Han SK, Jégu T, Archacki R, Van Leene J, Andriankaja M, De Bodt S, Abeel T, Coppens F, Dhondt S, De Milde L, Vermeersch M, Maleux K, Gevaert K, Jerzmanowski A, Benhamed M, Wagner D, Vandepoele K, De Jaeger G, Inzé D. - ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development
Visit the PubMed Articledeveloping first leaves of AN3-GR and wild-type plants were subjected to transcript profiling using Affymetrix ATH1 microarrays after transfer at 8 DAS to DEX-containing medium for 8 h ... four members of the GRF family were upregulated ... GRF8
Vercruyssen L, Verkest A, Gonzalez N, Heyndrickx KS, Eeckhout D, Han SK, Jégu T, Archacki R, Van Leene J, Andriankaja M, De Bodt S, Abeel T, Coppens F, Dhondt S, De Milde L, Vermeersch M, Maleux K, Gevaert K, Jerzmanowski A, Benhamed M, Wagner D, Vandepoele K, De Jaeger G, Inzé D. - ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development
Visit the PubMed ArticleqRT-PCR expression levels of ... GRF6 were found to be significantly reduced in 8-d-old brm3 shoots grown in long-day conditions compared with wild-type seedlings
Vercruyssen L, Verkest A, Gonzalez N, Heyndrickx KS, Eeckhout D, Han SK, Jégu T, Archacki R, Van Leene J, Andriankaja M, De Bodt S, Abeel T, Coppens F, Dhondt S, De Milde L, Vermeersch M, Maleux K, Gevaert K, Jerzmanowski A, Benhamed M, Wagner D, Vandepoele K, De Jaeger G, Inzé D. - ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development
Visit the PubMed ArticleTranscription factors with higher expression in ... AN3-GR ... leaves encoded the ... HOMEOBOX33 (HB33)
Vercruyssen L, Verkest A, Gonzalez N, Heyndrickx KS, Eeckhout D, Han SK, Jégu T, Archacki R, Van Leene J, Andriankaja M, De Bodt S, Abeel T, Coppens F, Dhondt S, De Milde L, Vermeersch M, Maleux K, Gevaert K, Jerzmanowski A, Benhamed M, Wagner D, Vandepoele K, De Jaeger G, Inzé D. - ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development
Visit the PubMed ArticleGRF3 ... not differentially expressed when brm was mutated
Vercruyssen L, Verkest A, Gonzalez N, Heyndrickx KS, Eeckhout D, Han SK, Jégu T, Archacki R, Van Leene J, Andriankaja M, De Bodt S, Abeel T, Coppens F, Dhondt S, De Milde L, Vermeersch M, Maleux K, Gevaert K, Jerzmanowski A, Benhamed M, Wagner D, Vandepoele K, De Jaeger G, Inzé D. - ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development
Visit the PubMed ArticleCRF2 ... downregulated in brm1 shoots
Vercruyssen L, Verkest A, Gonzalez N, Heyndrickx KS, Eeckhout D, Han SK, Jégu T, Archacki R, Van Leene J, Andriankaja M, De Bodt S, Abeel T, Coppens F, Dhondt S, De Milde L, Vermeersch M, Maleux K, Gevaert K, Jerzmanowski A, Benhamed M, Wagner D, Vandepoele K, De Jaeger G, Inzé D. - ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development
Visit the PubMed ArticleARR4 ... not differentially expressed when brm was mutated
Vercruyssen L, Verkest A, Gonzalez N, Heyndrickx KS, Eeckhout D, Han SK, Jégu T, Archacki R, Van Leene J, Andriankaja M, De Bodt S, Abeel T, Coppens F, Dhondt S, De Milde L, Vermeersch M, Maleux K, Gevaert K, Jerzmanowski A, Benhamed M, Wagner D, Vandepoele K, De Jaeger G, Inzé D. - ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development
Visit the PubMed ArticleTo analyze this in more detail, a time-course experiment was conducted, in which RNA levels were quantified with quantitative RT-PCR (qRT-PCR) at 1, 2, 4, and 6 h after transfer of AN3-GR and wild-type plants to DEX-containing medium ... transiently significantly upregulated ... after transfer to DEX, HEC1 after 2, 4, and 6 h
Vercruyssen L, Verkest A, Gonzalez N, Heyndrickx KS, Eeckhout D, Han SK, Jégu T, Archacki R, Van Leene J, Andriankaja M, De Bodt S, Abeel T, Coppens F, Dhondt S, De Milde L, Vermeersch M, Maleux K, Gevaert K, Jerzmanowski A, Benhamed M, Wagner D, Vandepoele K, De Jaeger G, Inzé D. - ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development
Visit the PubMed ArticleTranscription factors with higher expression in ... 35S:GRF5 leaves encoded ... HOMEOBOX33 (HB33)
Vercruyssen L, Verkest A, Gonzalez N, Heyndrickx KS, Eeckhout D, Han SK, Jégu T, Archacki R, Van Leene J, Andriankaja M, De Bodt S, Abeel T, Coppens F, Dhondt S, De Milde L, Vermeersch M, Maleux K, Gevaert K, Jerzmanowski A, Benhamed M, Wagner D, Vandepoele K, De Jaeger G, Inzé D. - ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development
Visit the PubMed ArticleARR4 is of interest because it was found to be upregulated in the an3 mutant
Vercruyssen L, Verkest A, Gonzalez N, Heyndrickx KS, Eeckhout D, Han SK, Jégu T, Archacki R, Van Leene J, Andriankaja M, De Bodt S, Abeel T, Coppens F, Dhondt S, De Milde L, Vermeersch M, Maleux K, Gevaert K, Jerzmanowski A, Benhamed M, Wagner D, Vandepoele K, De Jaeger G, Inzé D. - ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development
Visit the PubMed ArticleTo analyze this in more detail, a time-course experiment was conducted, in which RNA levels were quantified with quantitative RT-PCR (qRT-PCR) at 1, 2, 4, and 6 h after transfer of AN3-GR and wild-type plants to DEX-containing medium ... The transcript levels of GRF7 ... in general low and variable in the first leaves and not significantly affected by DEX treatment
Vercruyssen L, Verkest A, Gonzalez N, Heyndrickx KS, Eeckhout D, Han SK, Jégu T, Archacki R, Van Leene J, Andriankaja M, De Bodt S, Abeel T, Coppens F, Dhondt S, De Milde L, Vermeersch M, Maleux K, Gevaert K, Jerzmanowski A, Benhamed M, Wagner D, Vandepoele K, De Jaeger G, Inzé D. - ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development
Visit the PubMed ArticleThe CYCB1;1:DB-GUS construct allows for quantitative analysis of mitotic activity in developing plants. AN3-GR/CYCB1;1:DB-GUS plants were grown for 9 DAS and subsequently transferred to medium supplemented with 10 µM DEX for 24 h, after which the first leaves were analyzed for GUS staining. At this stage, mitotic activity is only present in the basal region in CYCB1;1:DB-GUS leaves (Figures 1D and 1E). Induction of AN3 activity extended the region of GUS staining measured along the length of the leaf while total leaf length was unaffected by 24-h DEX treatment (Figures 1D and 1E). In addition, the GUS intensity in the stained region was increased in AN3-GR/CYCB1;1:DB-GUS leaves compared with untreated and CYCB1;1:DB-GUS leaves (Figures 1E and 1F), indicating a function for AN3 in both the duration and the rate of cell proliferation
Vercruyssen L, Verkest A, Gonzalez N, Heyndrickx KS, Eeckhout D, Han SK, Jégu T, Archacki R, Van Leene J, Andriankaja M, De Bodt S, Abeel T, Coppens F, Dhondt S, De Milde L, Vermeersch M, Maleux K, Gevaert K, Jerzmanowski A, Benhamed M, Wagner D, Vandepoele K, De Jaeger G, Inzé D. - ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development
Visit the PubMed ArticleTo analyze this in more detail, a time-course experiment was conducted, in which RNA levels were quantified with quantitative RT-PCR (qRT-PCR) at 1, 2, 4, and 6 h after transfer of AN3-GR and wild-type plants to DEX-containing medium ... transiently significantly upregulated ... after transfer to DEX ... HB33 after 6 h
Vercruyssen L, Verkest A, Gonzalez N, Heyndrickx KS, Eeckhout D, Han SK, Jégu T, Archacki R, Van Leene J, Andriankaja M, De Bodt S, Abeel T, Coppens F, Dhondt S, De Milde L, Vermeersch M, Maleux K, Gevaert K, Jerzmanowski A, Benhamed M, Wagner D, Vandepoele K, De Jaeger G, Inzé D. - ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development
Visit the PubMed ArticleqRT-PCR expression levels of AN3 ... were found to be significantly reduced in 8-d-old brm3 shoots grown in long-day conditions compared with wild-type seedlings
Vercruyssen L, Verkest A, Gonzalez N, Heyndrickx KS, Eeckhout D, Han SK, Jégu T, Archacki R, Van Leene J, Andriankaja M, De Bodt S, Abeel T, Coppens F, Dhondt S, De Milde L, Vermeersch M, Maleux K, Gevaert K, Jerzmanowski A, Benhamed M, Wagner D, Vandepoele K, De Jaeger G, Inzé D. - ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development
Visit the PubMed ArticleTo analyze this in more detail, a time-course experiment was conducted, in which RNA levels were quantified with quantitative RT-PCR (qRT-PCR) at 1, 2, 4, and 6 h after transfer of AN3-GR and wild-type plants to DEX-containing medium ... ARR4 transcript levels were significantly lower in AN3-GR leaves 4 and 6 h after induction compared with the wild type (Figure 2B), confirming its rapid repression by AN3 in developing leaves
Vercruyssen L, Verkest A, Gonzalez N, Heyndrickx KS, Eeckhout D, Han SK, Jégu T, Archacki R, Van Leene J, Andriankaja M, De Bodt S, Abeel T, Coppens F, Dhondt S, De Milde L, Vermeersch M, Maleux K, Gevaert K, Jerzmanowski A, Benhamed M, Wagner D, Vandepoele K, De Jaeger G, Inzé D. - ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development
Visit the PubMed ArticleTo analyze this in more detail, a time-course experiment was conducted, in which RNA levels were quantified with quantitative RT-PCR (qRT-PCR) at 1, 2, 4, and 6 h after transfer of AN3-GR and wild-type plants to DEX-containing medium ... CYTOKININ RESPONSE FACTOR2 (CRF2) was transiently significantly upregulated 1 h after transfer to DEX
Vercruyssen L, Verkest A, Gonzalez N, Heyndrickx KS, Eeckhout D, Han SK, Jégu T, Archacki R, Van Leene J, Andriankaja M, De Bodt S, Abeel T, Coppens F, Dhondt S, De Milde L, Vermeersch M, Maleux K, Gevaert K, Jerzmanowski A, Benhamed M, Wagner D, Vandepoele K, De Jaeger G, Inzé D. - ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development
Visit the PubMed Articlewhen brm was mutated ... HB33, in contrast with our expectations, was upregulated
Vercruyssen L, Verkest A, Gonzalez N, Heyndrickx KS, Eeckhout D, Han SK, Jégu T, Archacki R, Van Leene J, Andriankaja M, De Bodt S, Abeel T, Coppens F, Dhondt S, De Milde L, Vermeersch M, Maleux K, Gevaert K, Jerzmanowski A, Benhamed M, Wagner D, Vandepoele K, De Jaeger G, Inzé D. - ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development
Visit the PubMed Articlein 12-d-old an3 rosettes ... GRF3 expression was unchanged compared with the wild type
Vercruyssen L, Verkest A, Gonzalez N, Heyndrickx KS, Eeckhout D, Han SK, Jégu T, Archacki R, Van Leene J, Andriankaja M, De Bodt S, Abeel T, Coppens F, Dhondt S, De Milde L, Vermeersch M, Maleux K, Gevaert K, Jerzmanowski A, Benhamed M, Wagner D, Vandepoele K, De Jaeger G, Inzé D. - ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development
Visit the PubMed ArticleTranscription of AN3 was also significantly reduced in brm1 rosettes grown for 22 d in short-day conditions
Vercruyssen L, Verkest A, Gonzalez N, Heyndrickx KS, Eeckhout D, Han SK, Jégu T, Archacki R, Van Leene J, Andriankaja M, De Bodt S, Abeel T, Coppens F, Dhondt S, De Milde L, Vermeersch M, Maleux K, Gevaert K, Jerzmanowski A, Benhamed M, Wagner D, Vandepoele K, De Jaeger G, Inzé D. - ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development
Visit the PubMed ArticleTo analyze this in more detail, a time-course experiment was conducted, in which RNA levels were quantified with quantitative RT-PCR (qRT-PCR) at 1, 2, 4, and 6 h after transfer of AN3-GR and wild-type plants to DEX-containing medium ... The transcript levels of ... GRF8 ... in general low and variable in the first leaves and not significantly affected by DEX treatment
Vercruyssen L, Verkest A, Gonzalez N, Heyndrickx KS, Eeckhout D, Han SK, Jégu T, Archacki R, Van Leene J, Andriankaja M, De Bodt S, Abeel T, Coppens F, Dhondt S, De Milde L, Vermeersch M, Maleux K, Gevaert K, Jerzmanowski A, Benhamed M, Wagner D, Vandepoele K, De Jaeger G, Inzé D. - ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development
Visit the PubMed ArticleTranscription factors with higher expression in ... AN3-GR ... leaves encoded the basic helix-loop-helix transcriptional regulator HECATE1 (HEC1)
Vercruyssen L, Verkest A, Gonzalez N, Heyndrickx KS, Eeckhout D, Han SK, Jégu T, Archacki R, Van Leene J, Andriankaja M, De Bodt S, Abeel T, Coppens F, Dhondt S, De Milde L, Vermeersch M, Maleux K, Gevaert K, Jerzmanowski A, Benhamed M, Wagner D, Vandepoele K, De Jaeger G, Inzé D. - ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development
Visit the PubMed ArticleHEC1 ... not differentially expressed when brm was mutated
Vercruyssen L, Verkest A, Gonzalez N, Heyndrickx KS, Eeckhout D, Han SK, Jégu T, Archacki R, Van Leene J, Andriankaja M, De Bodt S, Abeel T, Coppens F, Dhondt S, De Milde L, Vermeersch M, Maleux K, Gevaert K, Jerzmanowski A, Benhamed M, Wagner D, Vandepoele K, De Jaeger G, Inzé D. - ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development
Visit the PubMed Articledeveloping first leaves of AN3-GR and wild-type plants were subjected to transcript profiling using Affymetrix ATH1 microarrays after transfer at 8 DAS to DEX-containing medium for 8 h ... four members of the GRF family were upregulated: GRF3
Vercruyssen L, Verkest A, Gonzalez N, Heyndrickx KS, Eeckhout D, Han SK, Jégu T, Archacki R, Van Leene J, Andriankaja M, De Bodt S, Abeel T, Coppens F, Dhondt S, De Milde L, Vermeersch M, Maleux K, Gevaert K, Jerzmanowski A, Benhamed M, Wagner D, Vandepoele K, De Jaeger G, Inzé D. - ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development
Visit the PubMed ArticleTo analyze this in more detail, a time-course experiment was conducted, in which RNA levels were quantified with quantitative RT-PCR (qRT-PCR) at 1, 2, 4, and 6 h after transfer of AN3-GR and wild-type plants to DEX-containing medium ... GRF5 ... induced significantly from 2 h onwards
Vercruyssen L, Verkest A, Gonzalez N, Heyndrickx KS, Eeckhout D, Han SK, Jégu T, Archacki R, Van Leene J, Andriankaja M, De Bodt S, Abeel T, Coppens F, Dhondt S, De Milde L, Vermeersch M, Maleux K, Gevaert K, Jerzmanowski A, Benhamed M, Wagner D, Vandepoele K, De Jaeger G, Inzé D. - ANGUSTIFOLIA3 binds to SWI/SNF chromatin remodeling complexes to regulate transcription during Arabidopsis leaf development
Visit the PubMed Articlein yid1/med16-2 ... Under conditions of 50 µm Fe, we found no significant difference in IRT1 expression between mutants and wild-type plants under iron-sufficient conditions
Yang Y, Ou B, Zhang J, Si W, Gu H, Qin G, Qu LJ - The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25
Visit the PubMed Articleunder iron-deficient conditions, the transcriptional level of ... FRO2 was reduced in the yid1
Yang Y, Ou B, Zhang J, Si W, Gu H, Qin G, Qu LJ - The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25
Visit the PubMed Articlewe found that the induction of bHLH38 ... under iron-deficient conditions was unaffected
Yang Y, Ou B, Zhang J, Si W, Gu H, Qin G, Qu LJ - The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25
Visit the PubMed ArticleUnder conditions of 50 µm Fe ... FRO2 expression in the yid1 mutant was slightly lower compared with that in the wild type
Yang Y, Ou B, Zhang J, Si W, Gu H, Qin G, Qu LJ - The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25
Visit the PubMed ArticleFIT was significantly downregulated in ... med25 ... under iron-deficient conditions
Yang Y, Ou B, Zhang J, Si W, Gu H, Qin G, Qu LJ - The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25
Visit the PubMed Articleunder iron-deficient conditions, the transcriptional level of IRT1 ... was reduced in the yid1
Yang Y, Ou B, Zhang J, Si W, Gu H, Qin G, Qu LJ - The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25
Visit the PubMed ArticleYID1/MED16 was downregulated in the med25 mutant under iron-sufficient and iron-deficient conditions
Yang Y, Ou B, Zhang J, Si W, Gu H, Qin G, Qu LJ - The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25
Visit the PubMed ArticleQuantitative RT-PCR analysis showed that YID1/MED16 was expressed ubiquitously in different tissues
Yang Y, Ou B, Zhang J, Si W, Gu H, Qin G, Qu LJ - The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25
Visit the PubMed Articlein ... med25-2 mutants. Under conditions of 50 µm Fe, we found no significant difference in IRT1 expression between mutants and wild-type plants under iron-sufficient conditions
Yang Y, Ou B, Zhang J, Si W, Gu H, Qin G, Qu LJ - The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25
Visit the PubMed Articleunder iron-deficient conditions, the transcriptional level of ... FRO2 was reduced in the ... med25-2 mutant
Yang Y, Ou B, Zhang J, Si W, Gu H, Qin G, Qu LJ - The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25
Visit the PubMed Articlewe found that the induction of ... bHLH39 under iron-deficient conditions was unaffected
Yang Y, Ou B, Zhang J, Si W, Gu H, Qin G, Qu LJ - The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25
Visit the PubMed Articleunder iron-deficient conditions, the transcriptional level of IRT1 ... was reduced in the ... med25-2 mutant
Yang Y, Ou B, Zhang J, Si W, Gu H, Qin G, Qu LJ - The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25
Visit the PubMed ArticleFIT was significantly downregulated in yid1 ... under iron-deficient conditions
Yang Y, Ou B, Zhang J, Si W, Gu H, Qin G, Qu LJ - The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25
Visit the PubMed ArticleYID1/MED16 and MED25 were co-localized with the 4',6-diamidino-2-phenylindole (DAPI) signal from the nuclei
Yang Y, Ou B, Zhang J, Si W, Gu H, Qin G, Qu LJ - The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25
Visit the PubMed ArticleYID1/MED16 and MED25 were co-localized with the 4',6-diamidino-2-phenylindole (DAPI) signal from the nuclei
Yang Y, Ou B, Zhang J, Si W, Gu H, Qin G, Qu LJ - The Arabidopsis Mediator subunit MED16 regulates iron homeostasis by associating with EIN3/EIL1 through subunit MED25
Visit the PubMed ArticleWe also reexamined the expression of the Aux/IAA transcriptional regulator IAA2 by RT-PCR since this gene was identified as a potential KAN target in the original microarray experiment based on a 4-h DEX treatment (Supplemental Data Set 1) and since this gene showed high statistical significance for KAN1-GR downregulation in the RNA-seq experiment. We found that IAA2 was dramatically repressed by DEX in the presence of CHX, suggesting that it is a direct target of KAN-GR
Huang T, Harrar Y, Lin C, Reinhart B, Newell NR, Talavera-Rauh F, Hokin SA, Barton MK, Kerstetter RA - Arabidopsis KANADI1 acts as a transcriptional repressor by interacting with a specific cis-element and regulates auxin biosynthesis, transport, and signaling in opposition to HD-ZIPIII factors
Visit the PubMed Articlewe found evidence for regulation by KAN1-GR ... of ... NPY3
Huang T, Harrar Y, Lin C, Reinhart B, Newell NR, Talavera-Rauh F, Hokin SA, Barton MK, Kerstetter RA - Arabidopsis KANADI1 acts as a transcriptional repressor by interacting with a specific cis-element and regulates auxin biosynthesis, transport, and signaling in opposition to HD-ZIPIII factors
Visit the PubMed Articlewe found evidence for regulation by ... GR-REV of ... ENP1/NPY1
Huang T, Harrar Y, Lin C, Reinhart B, Newell NR, Talavera-Rauh F, Hokin SA, Barton MK, Kerstetter RA - Arabidopsis KANADI1 acts as a transcriptional repressor by interacting with a specific cis-element and regulates auxin biosynthesis, transport, and signaling in opposition to HD-ZIPIII factors
Visit the PubMed ArticleNPY1 showed downregulation by KAN1-GR in the microarray experiment, but this was not repeated in either the RNA-SEQ or qRT-PCR experiments
Huang T, Harrar Y, Lin C, Reinhart B, Newell NR, Talavera-Rauh F, Hokin SA, Barton MK, Kerstetter RA - Arabidopsis KANADI1 acts as a transcriptional repressor by interacting with a specific cis-element and regulates auxin biosynthesis, transport, and signaling in opposition to HD-ZIPIII factors
Visit the PubMed Articlewe found evidence for regulation by KAN1-GR ... of ... At3g15570
Huang T, Harrar Y, Lin C, Reinhart B, Newell NR, Talavera-Rauh F, Hokin SA, Barton MK, Kerstetter RA - Arabidopsis KANADI1 acts as a transcriptional repressor by interacting with a specific cis-element and regulates auxin biosynthesis, transport, and signaling in opposition to HD-ZIPIII factors
Visit the PubMed ArticleIAA2 was upregulated in kan1 ... mutant seedlings
Huang T, Harrar Y, Lin C, Reinhart B, Newell NR, Talavera-Rauh F, Hokin SA, Barton MK, Kerstetter RA - Arabidopsis KANADI1 acts as a transcriptional repressor by interacting with a specific cis-element and regulates auxin biosynthesis, transport, and signaling in opposition to HD-ZIPIII factors
Visit the PubMed ArticleARF3/ETTIN showed reproducible upregulation by GR-REV
Huang T, Harrar Y, Lin C, Reinhart B, Newell NR, Talavera-Rauh F, Hokin SA, Barton MK, Kerstetter RA - Arabidopsis KANADI1 acts as a transcriptional repressor by interacting with a specific cis-element and regulates auxin biosynthesis, transport, and signaling in opposition to HD-ZIPIII factors
Visit the PubMed Articlewe found evidence for regulation by ... GR-REV of ... At1g52770
Huang T, Harrar Y, Lin C, Reinhart B, Newell NR, Talavera-Rauh F, Hokin SA, Barton MK, Kerstetter RA - Arabidopsis KANADI1 acts as a transcriptional repressor by interacting with a specific cis-element and regulates auxin biosynthesis, transport, and signaling in opposition to HD-ZIPIII factors
Visit the PubMed ArticleIAA2 was upregulated in kan1 and further upregulated in kan1 kan2 mutant seedlings
Huang T, Harrar Y, Lin C, Reinhart B, Newell NR, Talavera-Rauh F, Hokin SA, Barton MK, Kerstetter RA - Arabidopsis KANADI1 acts as a transcriptional repressor by interacting with a specific cis-element and regulates auxin biosynthesis, transport, and signaling in opposition to HD-ZIPIII factors
Visit the PubMed ArticleIAA11 ... showed reproducible downregulation by KAN1-GR
Huang T, Harrar Y, Lin C, Reinhart B, Newell NR, Talavera-Rauh F, Hokin SA, Barton MK, Kerstetter RA - Arabidopsis KANADI1 acts as a transcriptional repressor by interacting with a specific cis-element and regulates auxin biosynthesis, transport, and signaling in opposition to HD-ZIPIII factors
Visit the PubMed Articlewe found evidence for regulation by KAN1-GR ... of ... PIN4
Huang T, Harrar Y, Lin C, Reinhart B, Newell NR, Talavera-Rauh F, Hokin SA, Barton MK, Kerstetter RA - Arabidopsis KANADI1 acts as a transcriptional repressor by interacting with a specific cis-element and regulates auxin biosynthesis, transport, and signaling in opposition to HD-ZIPIII factors
Visit the PubMed Articlewe found evidence for regulation by KAN1-GR ... of ... At3g08570
Huang T, Harrar Y, Lin C, Reinhart B, Newell NR, Talavera-Rauh F, Hokin SA, Barton MK, Kerstetter RA - Arabidopsis KANADI1 acts as a transcriptional repressor by interacting with a specific cis-element and regulates auxin biosynthesis, transport, and signaling in opposition to HD-ZIPIII factors
Visit the PubMed Articlewe found evidence for regulation by ... GR-REV of ... At5g47800
Huang T, Harrar Y, Lin C, Reinhart B, Newell NR, Talavera-Rauh F, Hokin SA, Barton MK, Kerstetter RA - Arabidopsis KANADI1 acts as a transcriptional repressor by interacting with a specific cis-element and regulates auxin biosynthesis, transport, and signaling in opposition to HD-ZIPIII factors
Visit the PubMed ArticleA third technique, qRT-PCR, on independent samples confirmed statistically significant upregulation of NPY1 by GR-REV in the presence and absence of CHX, indicating that NPY1 is likely a direct target of REV activation
Huang T, Harrar Y, Lin C, Reinhart B, Newell NR, Talavera-Rauh F, Hokin SA, Barton MK, Kerstetter RA - Arabidopsis KANADI1 acts as a transcriptional repressor by interacting with a specific cis-element and regulates auxin biosynthesis, transport, and signaling in opposition to HD-ZIPIII factors
Visit the PubMed Articlewe found evidence for regulation by KAN1-GR ... of ... IAA18
Huang T, Harrar Y, Lin C, Reinhart B, Newell NR, Talavera-Rauh F, Hokin SA, Barton MK, Kerstetter RA - Arabidopsis KANADI1 acts as a transcriptional repressor by interacting with a specific cis-element and regulates auxin biosynthesis, transport, and signaling in opposition to HD-ZIPIII factors
Visit the PubMed Articlewe found evidence for regulation by KAN1-GR ... of ... At5g47800
Huang T, Harrar Y, Lin C, Reinhart B, Newell NR, Talavera-Rauh F, Hokin SA, Barton MK, Kerstetter RA - Arabidopsis KANADI1 acts as a transcriptional repressor by interacting with a specific cis-element and regulates auxin biosynthesis, transport, and signaling in opposition to HD-ZIPIII factors
Visit the PubMed Articlewe found evidence for regulation by KAN1-GR ... of ... IAA11
Huang T, Harrar Y, Lin C, Reinhart B, Newell NR, Talavera-Rauh F, Hokin SA, Barton MK, Kerstetter RA - Arabidopsis KANADI1 acts as a transcriptional repressor by interacting with a specific cis-element and regulates auxin biosynthesis, transport, and signaling in opposition to HD-ZIPIII factors
Visit the PubMed ArticleqRT-PCR on an independent set of samples showed upregulation of WAG1 by GR-REV both in the presence and absence of CHX
Huang T, Harrar Y, Lin C, Reinhart B, Newell NR, Talavera-Rauh F, Hokin SA, Barton MK, Kerstetter RA - Arabidopsis KANADI1 acts as a transcriptional repressor by interacting with a specific cis-element and regulates auxin biosynthesis, transport, and signaling in opposition to HD-ZIPIII factors
Visit the PubMed ArticleNPY5 transcript levels were decreased by KAN1-GR in both microarray and RNA-seq experiments but were unchanged in the qRT-PCR experiments
Huang T, Harrar Y, Lin C, Reinhart B, Newell NR, Talavera-Rauh F, Hokin SA, Barton MK, Kerstetter RA - Arabidopsis KANADI1 acts as a transcriptional repressor by interacting with a specific cis-element and regulates auxin biosynthesis, transport, and signaling in opposition to HD-ZIPIII factors
Visit the PubMed Articlewe found evidence for regulation by ... GR-REV of ... ARF3
Huang T, Harrar Y, Lin C, Reinhart B, Newell NR, Talavera-Rauh F, Hokin SA, Barton MK, Kerstetter RA - Arabidopsis KANADI1 acts as a transcriptional repressor by interacting with a specific cis-element and regulates auxin biosynthesis, transport, and signaling in opposition to HD-ZIPIII factors
Visit the PubMed Articlewe found evidence for regulation by ... GR-REV of ... LAX3
Huang T, Harrar Y, Lin C, Reinhart B, Newell NR, Talavera-Rauh F, Hokin SA, Barton MK, Kerstetter RA - Arabidopsis KANADI1 acts as a transcriptional repressor by interacting with a specific cis-element and regulates auxin biosynthesis, transport, and signaling in opposition to HD-ZIPIII factors
Visit the PubMed Articlewe found evidence for regulation by KAN1-GR ... of ... LAX3
Huang T, Harrar Y, Lin C, Reinhart B, Newell NR, Talavera-Rauh F, Hokin SA, Barton MK, Kerstetter RA - Arabidopsis KANADI1 acts as a transcriptional repressor by interacting with a specific cis-element and regulates auxin biosynthesis, transport, and signaling in opposition to HD-ZIPIII factors
Visit the PubMed Articlewe found evidence for regulation by ... GR-REV of ... YUCCA5
Huang T, Harrar Y, Lin C, Reinhart B, Newell NR, Talavera-Rauh F, Hokin SA, Barton MK, Kerstetter RA - Arabidopsis KANADI1 acts as a transcriptional repressor by interacting with a specific cis-element and regulates auxin biosynthesis, transport, and signaling in opposition to HD-ZIPIII factors
Visit the PubMed Articlewe found evidence for regulation by ... GR-REV of ... LAX1
Huang T, Harrar Y, Lin C, Reinhart B, Newell NR, Talavera-Rauh F, Hokin SA, Barton MK, Kerstetter RA - Arabidopsis KANADI1 acts as a transcriptional repressor by interacting with a specific cis-element and regulates auxin biosynthesis, transport, and signaling in opposition to HD-ZIPIII factors
Visit the PubMed ArticleIAA18 showed reproducible downregulation by KAN1-GR
Huang T, Harrar Y, Lin C, Reinhart B, Newell NR, Talavera-Rauh F, Hokin SA, Barton MK, Kerstetter RA - Arabidopsis KANADI1 acts as a transcriptional repressor by interacting with a specific cis-element and regulates auxin biosynthesis, transport, and signaling in opposition to HD-ZIPIII factors
Visit the PubMed ArticleNPY3 transcripts showed statistically significant downregulation in response to KAN1-GR in all three experiments: microarray, qRT-PCR, and RNA-seq ... but NPY3 levels did not respond to KAN1-GR in the presence of CHX, indicating that KAN1-GR downregulation of NPY3 is likely an indirect effect
Huang T, Harrar Y, Lin C, Reinhart B, Newell NR, Talavera-Rauh F, Hokin SA, Barton MK, Kerstetter RA - Arabidopsis KANADI1 acts as a transcriptional repressor by interacting with a specific cis-element and regulates auxin biosynthesis, transport, and signaling in opposition to HD-ZIPIII factors
Visit the PubMed ArticleqRT-PCR on an independent set of samples showed ... downregulation of WAG2 by KAN1-GR but only in the absence of CHX
Huang T, Harrar Y, Lin C, Reinhart B, Newell NR, Talavera-Rauh F, Hokin SA, Barton MK, Kerstetter RA - Arabidopsis KANADI1 acts as a transcriptional repressor by interacting with a specific cis-element and regulates auxin biosynthesis, transport, and signaling in opposition to HD-ZIPIII factors
Visit the PubMed ArticleAt3g08570 ... upregulated by KAN1-GR
Huang T, Harrar Y, Lin C, Reinhart B, Newell NR, Talavera-Rauh F, Hokin SA, Barton MK, Kerstetter RA - Arabidopsis KANADI1 acts as a transcriptional repressor by interacting with a specific cis-element and regulates auxin biosynthesis, transport, and signaling in opposition to HD-ZIPIII factors
Visit the PubMed Articlewe found evidence for regulation by ... GR-REV of ... At1g50280
Huang T, Harrar Y, Lin C, Reinhart B, Newell NR, Talavera-Rauh F, Hokin SA, Barton MK, Kerstetter RA - Arabidopsis KANADI1 acts as a transcriptional repressor by interacting with a specific cis-element and regulates auxin biosynthesis, transport, and signaling in opposition to HD-ZIPIII factors
Visit the PubMed Articlewe found evidence for regulation by ... GR-REV of ... LAX2
Huang T, Harrar Y, Lin C, Reinhart B, Newell NR, Talavera-Rauh F, Hokin SA, Barton MK, Kerstetter RA - Arabidopsis KANADI1 acts as a transcriptional repressor by interacting with a specific cis-element and regulates auxin biosynthesis, transport, and signaling in opposition to HD-ZIPIII factors
Visit the PubMed Articlewe found evidence for regulation by KAN1-GR ... of ... NPY5
Huang T, Harrar Y, Lin C, Reinhart B, Newell NR, Talavera-Rauh F, Hokin SA, Barton MK, Kerstetter RA - Arabidopsis KANADI1 acts as a transcriptional repressor by interacting with a specific cis-element and regulates auxin biosynthesis, transport, and signaling in opposition to HD-ZIPIII factors
Visit the PubMed Articlewe found evidence for regulation by KAN1-GR ... of ... At3g19850
Huang T, Harrar Y, Lin C, Reinhart B, Newell NR, Talavera-Rauh F, Hokin SA, Barton MK, Kerstetter RA - Arabidopsis KANADI1 acts as a transcriptional repressor by interacting with a specific cis-element and regulates auxin biosynthesis, transport, and signaling in opposition to HD-ZIPIII factors
Visit the PubMed Articlewe found evidence for regulation by ... GR-REV of ... TAA1
Huang T, Harrar Y, Lin C, Reinhart B, Newell NR, Talavera-Rauh F, Hokin SA, Barton MK, Kerstetter RA - Arabidopsis KANADI1 acts as a transcriptional repressor by interacting with a specific cis-element and regulates auxin biosynthesis, transport, and signaling in opposition to HD-ZIPIII factors
Visit the PubMed Articlewe found evidence for regulation by KAN1-GR ... of ... LAX1
Huang T, Harrar Y, Lin C, Reinhart B, Newell NR, Talavera-Rauh F, Hokin SA, Barton MK, Kerstetter RA - Arabidopsis KANADI1 acts as a transcriptional repressor by interacting with a specific cis-element and regulates auxin biosynthesis, transport, and signaling in opposition to HD-ZIPIII factors
Visit the PubMed ArticleAPI2pro:GUS ... constructs were ubiquitously expressed, with particularly intense GUS staining in young and actively proliferating tissues, such as those of developing leaves
Casanova-Sáez R, Candela H, Micol JL - Combined haploinsufficiency and purifying selection drive retention of RPL36a paralogs in Arabidopsis
Visit the PubMed ArticleRPL36aApro:GUS ... constructs were ubiquitously expressed, with particularly intense GUS staining in young and actively proliferating tissues, such as those of developing leaves
Casanova-Sáez R, Candela H, Micol JL - Combined haploinsufficiency and purifying selection drive retention of RPL36a paralogs in Arabidopsis
Visit the PubMed Articlethe expression level of the nucleus-encoded ORESARA1 (ORE1) transcription factor, a senescence marker that has recently been reported to be a key regulator of the senescence response (Kim et al., 2009; Balazadeh et al., 2010), was also monitored. ORE1 was expressed at similar levels in anu10-1 mutants and wild-type rosettes (Fig. 9A), suggesting that premature senescence is not the cause of the observed phenotype
Casanova-Sáez R, Mateo-Bonmatí E, Kangasjärvi S, Candela H, Micol JL - Arabidopsis ANGULATA10 is required for thylakoid biogenesis and mesophyll development
Visit the PubMed ArticleThe relative expression of nuclear genes encoding subunits of LHCII trimers ... LHCB1 ... was found to be ~0.5-fold decreased in anu10-1 compared with Ler
Casanova-Sáez R, Mateo-Bonmatí E, Kangasjärvi S, Candela H, Micol JL - Arabidopsis ANGULATA10 is required for thylakoid biogenesis and mesophyll development
Visit the PubMed ArticleTo determine the expression pattern of ANU10 in wild-type plants, an ANU10 pro :GUS reporter transgene was constructed ... In plants collected 13 das, GUS activity was detected in ... cotyledons
Casanova-Sáez R, Mateo-Bonmatí E, Kangasjärvi S, Candela H, Micol JL - Arabidopsis ANGULATA10 is required for thylakoid biogenesis and mesophyll development
Visit the PubMed ArticleTo determine the expression pattern of ANU10 in wild-type plants, an ANU10 pro :GUS reporter transgene was constructed ... In plants collected 13 das, GUS activity was detected in ... leaves
Casanova-Sáez R, Mateo-Bonmatí E, Kangasjärvi S, Candela H, Micol JL - Arabidopsis ANGULATA10 is required for thylakoid biogenesis and mesophyll development
Visit the PubMed ArticleThe relative expression of nuclear genes encoding subunits of LHCII trimers ... LHCB2 ... was found to be ~0.5-fold decreased in anu10-1 compared with Ler
Casanova-Sáez R, Mateo-Bonmatí E, Kangasjärvi S, Candela H, Micol JL - Arabidopsis ANGULATA10 is required for thylakoid biogenesis and mesophyll development
Visit the PubMed Articletranscript levels of the plastid-encoded ... rrn23 ... were not significantly different from those of Ler ... anu10-1
Casanova-Sáez R, Mateo-Bonmatí E, Kangasjärvi S, Candela H, Micol JL - Arabidopsis ANGULATA10 is required for thylakoid biogenesis and mesophyll development
Visit the PubMed ArticleTo determine the suborganellar localization of the ANU10 protein, stromal and thylakoidal fractions were isolated from the chloroplasts of anu10-1 35S pro :ANU10:GFP transgenic plants. The presence of the ANU10:GFP fusion protein in each fraction was tested by western blotting using an anti-GFP antibody. The fusion protein was specifically detected in thylakoids, but not in the soluble stromal fraction (Fig. 5G), indicating that ANU10 is associated with thylakoidal membranes
Casanova-Sáez R, Mateo-Bonmatí E, Kangasjärvi S, Candela H, Micol JL - Arabidopsis ANGULATA10 is required for thylakoid biogenesis and mesophyll development
Visit the PubMed ArticleThe expression of HEMA1 was significantly although mildly reduced in anu10-1
Casanova-Sáez R, Mateo-Bonmatí E, Kangasjärvi S, Candela H, Micol JL - Arabidopsis ANGULATA10 is required for thylakoid biogenesis and mesophyll development
Visit the PubMed Articlethe expression levels of LHCB5 were not significantly different in anu10-1 and Ler
Casanova-Sáez R, Mateo-Bonmatí E, Kangasjärvi S, Candela H, Micol JL - Arabidopsis ANGULATA10 is required for thylakoid biogenesis and mesophyll development
Visit the PubMed Articletranscript levels of the plastid-encoded accD ... were not significantly different from those of Ler ... anu10-1
Casanova-Sáez R, Mateo-Bonmatí E, Kangasjärvi S, Candela H, Micol JL - Arabidopsis ANGULATA10 is required for thylakoid biogenesis and mesophyll development
Visit the PubMed ArticleA reduction only of rrn16 expression in anu10-1 mutants was observed
Casanova-Sáez R, Mateo-Bonmatí E, Kangasjärvi S, Candela H, Micol JL - Arabidopsis ANGULATA10 is required for thylakoid biogenesis and mesophyll development
Visit the PubMed Articletranscript levels of the plastid-encoded ... psbA ... were not significantly different from those of Ler ... anu10-1
Casanova-Sáez R, Mateo-Bonmatí E, Kangasjärvi S, Candela H, Micol JL - Arabidopsis ANGULATA10 is required for thylakoid biogenesis and mesophyll development
Visit the PubMed ArticleBecause LHCII trimers are thought to participate in thylakoid stacking, blue native PAGE was used to study the levels of trimeric LHCII complexes in isolated thylakoids from 16 das plants. LHCII trimers migrated slightly more slowly and their levels were reduced in thylakoids of anu10-1 compared with those of Ler (Fig. 8A, lanes 1 and 2). The anu10-2 and anu10-3 mutants showed a similar reduction in the amount and mobility of LHCII trimers relative to the Col-0 wild type
Casanova-Sáez R, Mateo-Bonmatí E, Kangasjärvi S, Candela H, Micol JL - Arabidopsis ANGULATA10 is required for thylakoid biogenesis and mesophyll development
Visit the PubMed ArticleTo determine experimentally the subcellular localization of the ANU10 protein, an in-frame translational fusion of GFP to the C-terminal end of ANU10 was made. Transgenic anu10-1 plants expressing 35S pro :ANU10:GFP were used to visualize the GFP signal by confocal laser scanning microscopy. The GFP signal was specifically detected in chloroplasts from four independent lines carrying the 35S pro :ANU10:GFP transgene (Fig. 5A–F). To exclude that the observed localization pattern represents an artefact due to overexpression of the ANU10:GFP fusion protein, the same translational fusion was also placed under the control of the endogenous ANU10 promoter (ANU10 pro :ANU10:GFP). An identical distribution of GFP signal was observed in anu10-1 plants expressing the ANU10 pro :ANU10:GFP transgene (Supplementary Fig. S5 at JXB online), demonstrating that the promoter chosen does not affect the subcellular localization of the fusion protein
Casanova-Sáez R, Mateo-Bonmatí E, Kangasjärvi S, Candela H, Micol JL - Arabidopsis ANGULATA10 is required for thylakoid biogenesis and mesophyll development
Visit the PubMed ArticleTo determine the expression pattern of ANU10 in wild-type plants, an ANU10 pro :GUS reporter transgene was constructed ... The spatio-temporal expression analysis suggests that ANU10 is expressed in all plant organs and that its expression is particularly important in developing organs
Casanova-Sáez R, Mateo-Bonmatí E, Kangasjärvi S, Candela H, Micol JL - Arabidopsis ANGULATA10 is required for thylakoid biogenesis and mesophyll development
Visit the PubMed Articletranscript levels of the plastid-encoded ... rbcL ... were not significantly different from those of Ler ... anu10-1
Casanova-Sáez R, Mateo-Bonmatí E, Kangasjärvi S, Candela H, Micol JL - Arabidopsis ANGULATA10 is required for thylakoid biogenesis and mesophyll development
Visit the PubMed ArticleTo determine the expression pattern of ANU10 in wild-type plants, an ANU10 pro :GUS reporter transgene was constructed ... The GUS signal was broad in seedlings collected 3 das (Fig. 4A), being particularly intense in incipient leaves
Casanova-Sáez R, Mateo-Bonmatí E, Kangasjärvi S, Candela H, Micol JL - Arabidopsis ANGULATA10 is required for thylakoid biogenesis and mesophyll development
Visit the PubMed ArticleThe relative expression of nuclear genes encoding subunits of LHCII trimers ... LHCB3 ... was found to be ~0.5-fold decreased in anu10-1 compared with Ler
Casanova-Sáez R, Mateo-Bonmatí E, Kangasjärvi S, Candela H, Micol JL - Arabidopsis ANGULATA10 is required for thylakoid biogenesis and mesophyll development
Visit the PubMed Articletranscript levels of the plastid-encoded ... atpB ... were not significantly different from those of Ler ... anu10-1
Casanova-Sáez R, Mateo-Bonmatí E, Kangasjärvi S, Candela H, Micol JL - Arabidopsis ANGULATA10 is required for thylakoid biogenesis and mesophyll development
Visit the PubMed Articlewe performed real-time PCR analysis using cDNA samples prepared from ... explants that were cultured on SIM at various temperatures after pre-culture on CIM at 19°C ... rid2 mutation ... elevated CUC1 ... with the maximum effect occurring at 25°C
Shinohara N, Ohbayashi I, Sugiyama M - Involvement of rRNA biosynthesis in the regulation of CUC1 gene expression and pre-meristematic cell mound formation during shoot regeneration
Visit the PubMed Articlewe performed real-time PCR analysis using cDNA samples prepared from ... explants that were cultured on SIM at various temperatures after pre-culture on CIM at 19°C ... rgd3 mutation did not elevate but suppress ... STM expressions
Shinohara N, Ohbayashi I, Sugiyama M - Involvement of rRNA biosynthesis in the regulation of CUC1 gene expression and pre-meristematic cell mound formation during shoot regeneration
Visit the PubMed Articlerid2 accumulated 20S pre-rRNA more abundantly than P(1)-A3 pre-rRNA
Shinohara N, Ohbayashi I, Sugiyama M - Involvement of rRNA biosynthesis in the regulation of CUC1 gene expression and pre-meristematic cell mound formation during shoot regeneration
Visit the PubMed Articlewe measured rRNA precursors in wild-type and rid3 plants grown at various temperatures by RNA gel blot analysis ... rRNA precursors of several different sizes such as 27S and 33S/35S accumulated to a very high level compared to the wild-type level in the ... rid3 plants grown under high temperature conditions ... The accumulation of rRNA precursors in rid3 was strongly influenced by the growth temperature and the unusually high-level accumulation occurred at higher temperatures
Shinohara N, Ohbayashi I, Sugiyama M - Involvement of rRNA biosynthesis in the regulation of CUC1 gene expression and pre-meristematic cell mound formation during shoot regeneration
Visit the PubMed ArticleExplants cultured on SIM at 19°C or 25°C for 5 or 8 days before GUS-signal detection were observed by light microscopy. In wild-type background explants cultured at 19°C for 5 days on SIM, strong GUS signals were detected in a broad area inside the calli and the signals locally extended to some spots of the callus surface. Those spots were mostly overlapped with mounds of relatively small cells. After 5 days culture at 25°C, wild-type background explants were forming SAMs, where GUS signal was strong at boundaries between the SAM and initiating leaf primordia and declined at the center. In rid2 background explants cultured at 19°C, two different patterns of GUS signal were observed: one was signal localized similarly to the pattern found in wild-type background explants, and the other was diffused or expanded signal pattern. When cultured at 25°C for 5 days, rid2 background explants showed ill-localized or diffused/expanded pattern of GUS signal, and after prolonged culture, they formed large mounds of GUS-positive cells. These results suggested that rid2 mutation may alter spatial control of CUC1 expression to lead to irregular development of cell mounds
Shinohara N, Ohbayashi I, Sugiyama M - Involvement of rRNA biosynthesis in the regulation of CUC1 gene expression and pre-meristematic cell mound formation during shoot regeneration
Visit the PubMed Articlewe performed real-time PCR analysis using cDNA samples prepared from ... explants that were cultured on SIM at various temperatures after pre-culture on CIM at 19°C ... rgd3 mutation did not elevate but suppress CUC1 ... expressions
Shinohara N, Ohbayashi I, Sugiyama M - Involvement of rRNA biosynthesis in the regulation of CUC1 gene expression and pre-meristematic cell mound formation during shoot regeneration
Visit the PubMed Articlerid2, a temperature-sensitive mutant that is mutated in a putative RNA methyltransferase gene and impaired in pre-rRNA processing (Ohbayashi et al., 2011 ... rRNA precursors of several different sizes such as 27S and 33S/35S accumulated to a very high level compared to the wild-type level in the rid2 ... grown under high temperature conditions
Shinohara N, Ohbayashi I, Sugiyama M - Involvement of rRNA biosynthesis in the regulation of CUC1 gene expression and pre-meristematic cell mound formation during shoot regeneration
Visit the PubMed Articlewe performed real-time PCR analysis using cDNA samples prepared from ... explants that were cultured on SIM at various temperatures after pre-culture on CIM at 19°C ... rid3 mutation elevated ... STM
Shinohara N, Ohbayashi I, Sugiyama M - Involvement of rRNA biosynthesis in the regulation of CUC1 gene expression and pre-meristematic cell mound formation during shoot regeneration
Visit the PubMed Articlerid3 accumulated P(1)-A3 pre-rRNA more abundantly than 20S pre-rRNA
Shinohara N, Ohbayashi I, Sugiyama M - Involvement of rRNA biosynthesis in the regulation of CUC1 gene expression and pre-meristematic cell mound formation during shoot regeneration
Visit the PubMed ArticleIn the wild type, rRNA precursor levels were slightly higher at lower temperatures, suggesting that changes in ambient temperature can affect rRNA biosynthesis
Shinohara N, Ohbayashi I, Sugiyama M - Involvement of rRNA biosynthesis in the regulation of CUC1 gene expression and pre-meristematic cell mound formation during shoot regeneration
Visit the PubMed Articlewe performed real-time PCR analysis using cDNA samples prepared from ... explants that were cultured on SIM at various temperatures after pre-culture on CIM at 19°C ... rid2 mutation ... elevated ... STM ... with the maximum effect occurring at 25°C
Shinohara N, Ohbayashi I, Sugiyama M - Involvement of rRNA biosynthesis in the regulation of CUC1 gene expression and pre-meristematic cell mound formation during shoot regeneration
Visit the PubMed Articlewe performed real-time PCR analysis using cDNA samples prepared from ... explants that were cultured on SIM at various temperatures after pre-culture on CIM at 19°C ... rid3 mutation elevated ... CUC1
Shinohara N, Ohbayashi I, Sugiyama M - Involvement of rRNA biosynthesis in the regulation of CUC1 gene expression and pre-meristematic cell mound formation during shoot regeneration
Visit the PubMed ArticleSEN4 ... we observed that in rGRF3 leaves there was no significant induction ... at 4 days after detaching
Debernardi JM, Mecchia MA, Vercruyssen L, Smaczniak C, Kaufmann K, Inze D, Rodriguez RE, Palatnik JF - Post-transcriptional control of GRF transcription factors by microRNA miR396 and GIF co-activator affects leaf size and longevity
Visit the PubMed Articletranscript levels of ... CYCLINB1;1 in rGRF3x35S:GIF1 leaves were at least two-fold higher than in wild-type plants
Debernardi JM, Mecchia MA, Vercruyssen L, Smaczniak C, Kaufmann K, Inze D, Rodriguez RE, Palatnik JF - Post-transcriptional control of GRF transcription factors by microRNA miR396 and GIF co-activator affects leaf size and longevity
Visit the PubMed ArticleThe level of these genes was evaluated by quantitative reverse transcription polymerase chain reaction (RT-qPCR) in detached leaves at different days, where senescence was induced by darkness ... SEN1 ... levels increased at 2 days after incubation in wild-type plants, and this increase was higher at 4 days
Debernardi JM, Mecchia MA, Vercruyssen L, Smaczniak C, Kaufmann K, Inze D, Rodriguez RE, Palatnik JF - Post-transcriptional control of GRF transcription factors by microRNA miR396 and GIF co-activator affects leaf size and longevity
Visit the PubMed Articletranscript levels of KNOLLE ... in rGRF3x35S:GIF1 leaves were at least two-fold higher than in wild-type plants
Debernardi JM, Mecchia MA, Vercruyssen L, Smaczniak C, Kaufmann K, Inze D, Rodriguez RE, Palatnik JF - Post-transcriptional control of GRF transcription factors by microRNA miR396 and GIF co-activator affects leaf size and longevity
Visit the PubMed ArticleThe level of these genes was evaluated by quantitative reverse transcription polymerase chain reaction (RT-qPCR) in detached leaves at different days, where senescence was induced by darkness ... We observed a greater induction of ... SEN4 in 35S:miR396 plants
Debernardi JM, Mecchia MA, Vercruyssen L, Smaczniak C, Kaufmann K, Inze D, Rodriguez RE, Palatnik JF - Post-transcriptional control of GRF transcription factors by microRNA miR396 and GIF co-activator affects leaf size and longevity
Visit the PubMed ArticleThe level of these genes was evaluated by quantitative reverse transcription polymerase chain reaction (RT-qPCR) in detached leaves at different days, where senescence was induced by darkness ... We observed a greater induction of ... SEN1 ... in 35S:miR396 plants
Debernardi JM, Mecchia MA, Vercruyssen L, Smaczniak C, Kaufmann K, Inze D, Rodriguez RE, Palatnik JF - Post-transcriptional control of GRF transcription factors by microRNA miR396 and GIF co-activator affects leaf size and longevity
Visit the PubMed Articlethe proliferation markers were also slightly higher in rGRF3 plants (approximately 1.5-fold for KNOLLE)
Debernardi JM, Mecchia MA, Vercruyssen L, Smaczniak C, Kaufmann K, Inze D, Rodriguez RE, Palatnik JF - Post-transcriptional control of GRF transcription factors by microRNA miR396 and GIF co-activator affects leaf size and longevity
Visit the PubMed ArticleFirst, we generated a GRF3:GUS reporter and found that the promoter of GRF3 was active constitutively during leaf development
Debernardi JM, Mecchia MA, Vercruyssen L, Smaczniak C, Kaufmann K, Inze D, Rodriguez RE, Palatnik JF - Post-transcriptional control of GRF transcription factors by microRNA miR396 and GIF co-activator affects leaf size and longevity
Visit the PubMed ArticleThe level of these genes was evaluated by quantitative reverse transcription polymerase chain reaction (RT-qPCR) in detached leaves at different days, where senescence was induced by darkness ... SEN4 levels increased at 2 days after incubation in wild-type plants, and this increase was higher at 4 days
Debernardi JM, Mecchia MA, Vercruyssen L, Smaczniak C, Kaufmann K, Inze D, Rodriguez RE, Palatnik JF - Post-transcriptional control of GRF transcription factors by microRNA miR396 and GIF co-activator affects leaf size and longevity
Visit the PubMed ArticleThe level of these genes was evaluated by quantitative reverse transcription polymerase chain reaction (RT-qPCR) in detached leaves at different days, where senescence was induced by darkness ... in wild-type plants ... SAG12 level was strongly increased at the fourth day after incubation
Debernardi JM, Mecchia MA, Vercruyssen L, Smaczniak C, Kaufmann K, Inze D, Rodriguez RE, Palatnik JF - Post-transcriptional control of GRF transcription factors by microRNA miR396 and GIF co-activator affects leaf size and longevity
Visit the PubMed ArticleSEN1 ... we observed that in rGRF3 leaves there was no significant induction ... at 4 days after detaching
Debernardi JM, Mecchia MA, Vercruyssen L, Smaczniak C, Kaufmann K, Inze D, Rodriguez RE, Palatnik JF - Post-transcriptional control of GRF transcription factors by microRNA miR396 and GIF co-activator affects leaf size and longevity
Visit the PubMed ArticleTo increase our understanding of how defects in thylakoid membrane biogenesis caused by the loss-of-function of FtsHi4 affected PSII protein accumulation in the ftshi4 mutant, immunoblot analyses were conducted using the thylakoid proteins extracted from green and white ovules isolated from the heterozygous ftshi4-1 mutant plants. Accumulation of the FtsHi4 protein was non-detectable in the white ovules, and the ... PSII ... PsbO ... decreased significantly
Lu X, Zhang D, Li S, Su Y, Liang Q, Meng H, Shen S, Fan Y, Liu C, Zhang C - FtsHi4 is essential for embryogenesis due to its influence on chloroplast development in Arabidopsis
Visit the PubMed ArticleFtsHi4 transcripts were most abundant in young leaves and present at the lowest levels in roots
Lu X, Zhang D, Li S, Su Y, Liang Q, Meng H, Shen S, Fan Y, Liu C, Zhang C - FtsHi4 is essential for embryogenesis due to its influence on chloroplast development in Arabidopsis
Visit the PubMed ArticleFtsHi4-RNAi mutant ... No significant changes were found for ... PsbO ... in leaves
Lu X, Zhang D, Li S, Su Y, Liang Q, Meng H, Shen S, Fan Y, Liu C, Zhang C - FtsHi4 is essential for embryogenesis due to its influence on chloroplast development in Arabidopsis
Visit the PubMed ArticleFtsHi4-RNAi mutant ... levels of ... PsbO (the subunit of the oxygen-evolving complex ... were significantly reduced in cotyledons of the RNAi plants compared with WT
Lu X, Zhang D, Li S, Su Y, Liang Q, Meng H, Shen S, Fan Y, Liu C, Zhang C - FtsHi4 is essential for embryogenesis due to its influence on chloroplast development in Arabidopsis
Visit the PubMed ArticleTo further investigate sub-organelle localization of the FtsHi4 protein, the thylakoid membrane fractions were isolated from wild-type plants and treated with alkaline and chaotropic salts to release membrane-associated proteins [40]. In this assay, PsbO (a 33-kDa luminal protein of PSII) and CP47 (a core protein of PSII) were used as markers to distinguish peripheral and integral membrane proteins, respectively. We found that FtsHi4 was retained in the membrane fraction, behaving similar to the integral protein CP47 (Fig. 4B). These results indicated that FtsHi4 was localized to the thylakoid as an integral membrane protein, even though it contains no predicted transmembrane domains
Lu X, Zhang D, Li S, Su Y, Liang Q, Meng H, Shen S, Fan Y, Liu C, Zhang C - FtsHi4 is essential for embryogenesis due to its influence on chloroplast development in Arabidopsis
Visit the PubMed ArticleWe also analyzed thylakoid membrane protein accumulation of ... PsbO with specific antibodies, and found that protein levels of the RNAi-FtsHi4 mutant decreased significantly compared with wild type
Lu X, Zhang D, Li S, Su Y, Liang Q, Meng H, Shen S, Fan Y, Liu C, Zhang C - FtsHi4 is essential for embryogenesis due to its influence on chloroplast development in Arabidopsis
Visit the PubMed ArticleFtsHi4-RNAi mutant ... levels of ... D2 ... were significantly reduced in cotyledons of the RNAi plants compared with WT
Lu X, Zhang D, Li S, Su Y, Liang Q, Meng H, Shen S, Fan Y, Liu C, Zhang C - FtsHi4 is essential for embryogenesis due to its influence on chloroplast development in Arabidopsis
Visit the PubMed ArticleFtsHi4-RNAi mutant ... levels of ... CP47 ... were significantly reduced in cotyledons of the RNAi plants compared with WT
Lu X, Zhang D, Li S, Su Y, Liang Q, Meng H, Shen S, Fan Y, Liu C, Zhang C - FtsHi4 is essential for embryogenesis due to its influence on chloroplast development in Arabidopsis
Visit the PubMed ArticleChloroplast thylakoid membranes contain a large number of proteins and protein complexes, such as photosystem I (PSI) and PSII, which play various roles in photosynthesis. FtsH proteins were previously shown to be localized near PSII at grana, and are responsible for turnover of the PSII D1 protein [28], [45]. Additionally, FtsHi4 shows a high degree of similarity to FtsH proteins [33]. Thus, we investigated whether FtsHi4 was also localized to PSII-enriched thylakoid membranes. To accomplish this, the thylakoid membrane protein complexes isolated from 4-week-old leaves were separated on blue native (BN)-PAGE gel, and the separated protein complexes corresponding to PSII supercomplexes (band I), monomeric PSI and dimeric PSII (band II), monomeric PSII (band III), dimeric cytochrome b6/f dimer (band IV), trimeric LHCII (band V), and monomeric LHCII (band VI) were fractionated by SDS-PAGE followed by immunoblotting using anti-FtsHi4 and -D2 antibodies. D2 protein is reported to assemble into the PSII complex [46]. Figure 5 showed that the D2 protein was mainly found at band II and III, and the FtsHi4 protein migrated in two protein complexes of ~460 and ~240 kDa, respectively, which also corresponded to the PSII dimer and monomer (Fig. 5A). Although some of the D2 complexes migrated at sizes similar to FtsHi4, the overall pattern differed between complexes. These results suggested that FtsHi4 forms a complex
Lu X, Zhang D, Li S, Su Y, Liang Q, Meng H, Shen S, Fan Y, Liu C, Zhang C - FtsHi4 is essential for embryogenesis due to its influence on chloroplast development in Arabidopsis
Visit the PubMed ArticleFtsHi4-RNAi mutant ... No significant changes were found for ... Cyt f in leaves
Lu X, Zhang D, Li S, Su Y, Liang Q, Meng H, Shen S, Fan Y, Liu C, Zhang C - FtsHi4 is essential for embryogenesis due to its influence on chloroplast development in Arabidopsis
Visit the PubMed ArticleFtsHi4-RNAi mutant ... CP47 showed a reduction in leaves, but to a lesser degree than in cotyledons
Lu X, Zhang D, Li S, Su Y, Liang Q, Meng H, Shen S, Fan Y, Liu C, Zhang C - FtsHi4 is essential for embryogenesis due to its influence on chloroplast development in Arabidopsis
Visit the PubMed ArticleWe also analyzed thylakoid membrane protein accumulation of D2 ... with specific antibodies, and found that protein levels of the RNAi-FtsHi4 mutant decreased significantly compared with wild type
Lu X, Zhang D, Li S, Su Y, Liang Q, Meng H, Shen S, Fan Y, Liu C, Zhang C - FtsHi4 is essential for embryogenesis due to its influence on chloroplast development in Arabidopsis
Visit the PubMed ArticleTo increase our understanding of how defects in thylakoid membrane biogenesis caused by the loss-of-function of FtsHi4 affected PSII protein accumulation in the ftshi4 mutant, immunoblot analyses were conducted using the thylakoid proteins extracted from green and white ovules isolated from the heterozygous ftshi4-1 mutant plants. Accumulation of the FtsHi4 protein was non-detectable in the white ovules, and the ... PSII ... D2 ... completely lost
Lu X, Zhang D, Li S, Su Y, Liang Q, Meng H, Shen S, Fan Y, Liu C, Zhang C - FtsHi4 is essential for embryogenesis due to its influence on chloroplast development in Arabidopsis
Visit the PubMed ArticleFtsHi4-RNAi mutant ... D1 ... showed a reduction in leaves, but to a lesser degree than in cotyledons
Lu X, Zhang D, Li S, Su Y, Liang Q, Meng H, Shen S, Fan Y, Liu C, Zhang C - FtsHi4 is essential for embryogenesis due to its influence on chloroplast development in Arabidopsis
Visit the PubMed ArticleTo confirm localization, the N-terminal polypeptide of FtsHi4 containing the targeting signal peptide was fused to the N-terminus of green fluorescent protein (GFP) under control of the constitutive CaMV 35S promoter. The resulting construct was introduced into Arabidopsis leaf protoplasts. Green fluorescent signals were co-localized with the chlorophyll autofluorescence in the transformed protoplasts, whereas control GFP lacking the FtsHi4 signal peptide was retained in the cytosol (Fig. 4A). These results indicated that FtsHi4 was targeted to chloroplasts
Lu X, Zhang D, Li S, Su Y, Liang Q, Meng H, Shen S, Fan Y, Liu C, Zhang C - FtsHi4 is essential for embryogenesis due to its influence on chloroplast development in Arabidopsis
Visit the PubMed ArticleFtsHi4-RNAi mutant ... levels of ... CP43 ... were significantly reduced in cotyledons of the RNAi plants compared with WT
Lu X, Zhang D, Li S, Su Y, Liang Q, Meng H, Shen S, Fan Y, Liu C, Zhang C - FtsHi4 is essential for embryogenesis due to its influence on chloroplast development in Arabidopsis
Visit the PubMed ArticleExpression analyses of the FtsHi4 gene in Arabidopsis using qRT-PCR with gene-specific primers showed that FtsHi4 transcripts were ubiquitously present in organs, including ... cotyledons
Lu X, Zhang D, Li S, Su Y, Liang Q, Meng H, Shen S, Fan Y, Liu C, Zhang C - FtsHi4 is essential for embryogenesis due to its influence on chloroplast development in Arabidopsis
Visit the PubMed ArticleFtsHi4-RNAi mutant ... CP43 ... showed a reduction in leaves, but to a lesser degree than in cotyledons
Lu X, Zhang D, Li S, Su Y, Liang Q, Meng H, Shen S, Fan Y, Liu C, Zhang C - FtsHi4 is essential for embryogenesis due to its influence on chloroplast development in Arabidopsis
Visit the PubMed ArticleFtsHi4-RNAi mutant ... levels of ... Cyt f (the subunit of cytochrome b6f complex ... were significantly reduced in cotyledons of the RNAi plants compared with WT
Lu X, Zhang D, Li S, Su Y, Liang Q, Meng H, Shen S, Fan Y, Liu C, Zhang C - FtsHi4 is essential for embryogenesis due to its influence on chloroplast development in Arabidopsis
Visit the PubMed ArticleFtsHi4-RNAi mutant ... levels of D1 ... were significantly reduced in cotyledons of the RNAi plants compared with WT
Lu X, Zhang D, Li S, Su Y, Liang Q, Meng H, Shen S, Fan Y, Liu C, Zhang C - FtsHi4 is essential for embryogenesis due to its influence on chloroplast development in Arabidopsis
Visit the PubMed ArticleExpression analyses of the FtsHi4 gene in Arabidopsis using qRT-PCR with gene-specific primers showed that FtsHi4 transcripts were ubiquitously present in organs, including ... rosette leaves
Lu X, Zhang D, Li S, Su Y, Liang Q, Meng H, Shen S, Fan Y, Liu C, Zhang C - FtsHi4 is essential for embryogenesis due to its influence on chloroplast development in Arabidopsis
Visit the PubMed ArticleFtsHi4-RNAi mutant ... No significant changes were found for D2 ... in leaves
Lu X, Zhang D, Li S, Su Y, Liang Q, Meng H, Shen S, Fan Y, Liu C, Zhang C - FtsHi4 is essential for embryogenesis due to its influence on chloroplast development in Arabidopsis
Visit the PubMed ArticleExpression analyses of the FtsHi4 gene in Arabidopsis using qRT-PCR with gene-specific primers showed that FtsHi4 transcripts were ubiquitously present in organs, including ... young true leaves
Lu X, Zhang D, Li S, Su Y, Liang Q, Meng H, Shen S, Fan Y, Liu C, Zhang C - FtsHi4 is essential for embryogenesis due to its influence on chloroplast development in Arabidopsis
Visit the PubMed ArticlePSI2 ... coding sequences were fused in frame to YFP and cloned behind the 35S promoter. Transient expression of 35S:PSI-YFP was studied in mesophyll protoplasts isolated from Arabidopsis leaves. Nuclei were visualized by DAPI staining. Fluorescence imaging and overlay pictures revealed that ... PSI proteins were localized to the nucleus
Stührwohldt N, Hartmann J, Dahlke RI, Oecking C, Sauter M - The PSI family of nuclear proteins is required for growth in arabidopsis
Visit the PubMed ArticlePSI3 coding sequences were fused in frame to YFP and cloned behind the 35S promoter. Transient expression of 35S:PSI-YFP was studied in mesophyll protoplasts isolated from Arabidopsis leaves. Nuclei were visualized by DAPI staining. Fluorescence imaging and overlay pictures revealed that ... PSI proteins were localized to the nucleus
Stührwohldt N, Hartmann J, Dahlke RI, Oecking C, Sauter M - The PSI family of nuclear proteins is required for growth in arabidopsis
Visit the PubMed ArticlePSI1 ... coding sequences were fused in frame to YFP and cloned behind the 35S promoter. Transient expression of 35S:PSI-YFP was studied in mesophyll protoplasts isolated from Arabidopsis leaves. Nuclei were visualized by DAPI staining. Fluorescence imaging and overlay pictures revealed that ... PSI proteins were localized to the nucleus
Stührwohldt N, Hartmann J, Dahlke RI, Oecking C, Sauter M - The PSI family of nuclear proteins is required for growth in arabidopsis
Visit the PubMed ArticleNext we used RT–PCR assays to ask whether AtMKK2 and AtMPK10 have an overlapping expression profile and could function together at the same time. As presented in Figure 2C, expression of MPK10 and MKK2 overlapped in developing leaves, but only in a very narrow time window from 2 to 12 DAG with a strikingly sharp peak at 4 DAG
Stanko V, Giuliani C, Retzer K, Djamei A, Wahl V, Wurzinger B, Wilson C, Heberle-Bors E, Teige M, Kragler F - Timing is everything: highly specific and transient expression of a MAP kinase determines auxin-induced leaf venation patterns in Arabidopsis
Visit the PubMed Articlewe performed RT–PCR assays on seedlings. As with GUS assays, we detected high AtMPK10 mRNA signals 2 DAG, which decreased 4 DAG and remained very low at later stages
Stanko V, Giuliani C, Retzer K, Djamei A, Wahl V, Wurzinger B, Wilson C, Heberle-Bors E, Teige M, Kragler F - Timing is everything: highly specific and transient expression of a MAP kinase determines auxin-induced leaf venation patterns in Arabidopsis
Visit the PubMed ArticleThe predicted 700-bp-long AtMPK10 promoter region was cloned into a glucuronidase (GUS) reporter binary vector to generate transgenic lines ... In 12- to 28-day old plants, GUS staining was found to coincide with small regions of minor veins, the marginal vasculature, the leaf tips, and the tips of the serrated margin of rosette leaves where veins meet
Stanko V, Giuliani C, Retzer K, Djamei A, Wahl V, Wurzinger B, Wilson C, Heberle-Bors E, Teige M, Kragler F - Timing is everything: highly specific and transient expression of a MAP kinase determines auxin-induced leaf venation patterns in Arabidopsis
Visit the PubMed Articleexpression analysis using an 801-bp-long promoter region of MKK2 in a binary GUS reporter construct revealed also a partially overlapping expression pattern of MPK10 and MKK2 in leaves, with generally higher levels and a wider local distribution for MKK2
Stanko V, Giuliani C, Retzer K, Djamei A, Wahl V, Wurzinger B, Wilson C, Heberle-Bors E, Teige M, Kragler F - Timing is everything: highly specific and transient expression of a MAP kinase determines auxin-induced leaf venation patterns in Arabidopsis
Visit the PubMed ArticleThe predicted 700-bp-long AtMPK10 promoter region was cloned into a glucuronidase (GUS) reporter binary vector to generate transgenic lines. Eight independent transgenic lines were isolated and analyzed by histochemical GUS enzyme activity assays starting from 1-day-old seedlings to 28-day-old plants ( Figure 1A–1I). No GUS activity could be detected 1 d after germination (DAG) ( Figure 1A). GUS activity was observed in the tips of the cotyledons at 2 DAG and, from 4 to 12 DAG, the signal appeared at the margins of cotyledons and in a patchy manner along their veins
Stanko V, Giuliani C, Retzer K, Djamei A, Wahl V, Wurzinger B, Wilson C, Heberle-Bors E, Teige M, Kragler F - Timing is everything: highly specific and transient expression of a MAP kinase determines auxin-induced leaf venation patterns in Arabidopsis
Visit the PubMed ArticleThe increase of AtMPK10 transcriptional activity in wild-type seedlings exposed to HFCA was confirmed by RT–PCR. A higher but transient AtMPK10 signal was detected at 4 and 8 DAG after exposure to 5 μM and 40 μM HFCA ( Figure 5K) relative to untreated seedlings ( Figure 2B) that disappeared at 12 DAG. AtMPK10 expression showed an increase at all time points tested upon treatment with 100 μM HFCA, congruent with the higher GUS signal in ProAtMPK10::GUS plants
Stanko V, Giuliani C, Retzer K, Djamei A, Wahl V, Wurzinger B, Wilson C, Heberle-Bors E, Teige M, Kragler F - Timing is everything: highly specific and transient expression of a MAP kinase determines auxin-induced leaf venation patterns in Arabidopsis
Visit the PubMed ArticleFirst, we tested whether the AtMPK10 promoter activity was controlled by auxin. ProAtMPK10::GUS plants grown on MS medium were treated with increasing concentrations of indole acetic acid (IAA) and AtMPK10 expression was followed by GUS staining ( Figure 5A– 5D). For this purpose, plants were either grown on MS medium containing 1 μM IAA ( Figure 5A) or were infiltrated 7 DAG with 10 μM or 100 μM IAA and tested for GUS activity 1 d later ( Figure 5C). In all instances, no evident changes in AtMPK10 promoter-driven GUS activity were observed ( Figure 5A– 5D), which was confirmed by semi-quantitative RT–PCR on wild-type plants (data not shown). These observations implied that AtMPK10 expression is not regulated by auxin
Stanko V, Giuliani C, Retzer K, Djamei A, Wahl V, Wurzinger B, Wilson C, Heberle-Bors E, Teige M, Kragler F - Timing is everything: highly specific and transient expression of a MAP kinase determines auxin-induced leaf venation patterns in Arabidopsis
Visit the PubMed ArticleNext, to uncover a link between AtMPK10 expression and PAT, we exposed ProAtMPK10::GUS plants to different concentrations of the PAT inhibitor HFCA. To visualize the effect of PAT inhibition, we used the well-characterized auxin reporter line ProDR5::GFP ( Benkova et al., 2003; Friml et al., 2003) and compared the GFP signal (indicating auxin concentrations) to AtMPK10 promoter-driven GUS expression ( Figure 5E– 5J). In control ProAtMPK10::GUS plants, GUS activity was highest in cotyledons at the distal margin of the midvein ( Figure 5B) and at the hydathodes in fully expanded rosette leaves ( Figure 5D). Presence of low amounts of HFCA (5 μM) resulted in an increased ProAtMPK10::GUS activity at the midvein and the tip ( Figure 5E) that did not coincide with an increased expression of the ProDR5::GFP auxin sensing lines ( Figure 5F). Rather, ProDR5::GFP plants showed an increased GFP signal at the leaf margins and veins ( Figure 5F). Treatment of both transgenic reporter lines with higher amounts of HFCA (40 μM and 100 μM) caused higher GUS and GFP activity in cotyledons and thicker, misshaped veins, a reduction in cotyledon size, and an elongation of rosette leaves ( Figure 5G– 5J). In general, ProAtMPK10::GUS activity coincided with the GFP signal from the auxin reporter line at the leaf tip, the leaf margins, and vasculature ( Figure 5F, 5H, and 5J, and Supplemenal Figure 2), similarly to that described for auxin distribution ( Mattsson et al., 1999; Sieburth, 1999; Mattsson et al., 2003). Thus, inhibition of auxin transport increased the area of auxin maxima sites in the leaf concomitantly with an increase in AtMPK10 expression
Stanko V, Giuliani C, Retzer K, Djamei A, Wahl V, Wurzinger B, Wilson C, Heberle-Bors E, Teige M, Kragler F - Timing is everything: highly specific and transient expression of a MAP kinase determines auxin-induced leaf venation patterns in Arabidopsis
Visit the PubMed ArticleDuring embryogenesis, consistently with online available expression data ( Winter et al., 2007), AtMPK10 expression seems to be restricted to the globular stage and was extremely low in RNA in situ assays
Stanko V, Giuliani C, Retzer K, Djamei A, Wahl V, Wurzinger B, Wilson C, Heberle-Bors E, Teige M, Kragler F - Timing is everything: highly specific and transient expression of a MAP kinase determines auxin-induced leaf venation patterns in Arabidopsis
Visit the PubMed ArticleThe predicted 700-bp-long AtMPK10 promoter region was cloned into a glucuronidase (GUS) reporter binary vector to generate transgenic lines ... 12- to 28-day old plants, GUS staining was found to coincide with ... hydathodes
Stanko V, Giuliani C, Retzer K, Djamei A, Wahl V, Wurzinger B, Wilson C, Heberle-Bors E, Teige M, Kragler F - Timing is everything: highly specific and transient expression of a MAP kinase determines auxin-induced leaf venation patterns in Arabidopsis
Visit the PubMed Articlehua2–7 hulk1 ... several other genes with known roles in flowering time regulation displayed decreased expression levels, such as MAF1
Jali SS, Rosloski SM, Janakirama P, Steffen JG, Zhurov V, Berleth T, Clark RM, Grbic V - A plant-specific HUA2-LIKE (HULK) gene family in Arabidopsis thaliana is essential for development
Visit the PubMed Articlehua2–7 hulk1 ... FLC is down-regulated
Jali SS, Rosloski SM, Janakirama P, Steffen JG, Zhurov V, Berleth T, Clark RM, Grbic V - A plant-specific HUA2-LIKE (HULK) gene family in Arabidopsis thaliana is essential for development
Visit the PubMed ArticleHULK3 ... was expressed broadly in vegetative and reproductive apices in domains that are essentially indistinguishable from those for HUA2
Jali SS, Rosloski SM, Janakirama P, Steffen JG, Zhurov V, Berleth T, Clark RM, Grbic V - A plant-specific HUA2-LIKE (HULK) gene family in Arabidopsis thaliana is essential for development
Visit the PubMed Articlehua2–7 ... several other genes with known roles in flowering time regulation displayed decreased expression levels, such as ... TEM1
Jali SS, Rosloski SM, Janakirama P, Steffen JG, Zhurov V, Berleth T, Clark RM, Grbic V - A plant-specific HUA2-LIKE (HULK) gene family in Arabidopsis thaliana is essential for development
Visit the PubMed ArticleHUA2 ... Based on both AtGenExpress (Schmid et al., 2005) and deep RNA-Seq read data from various tissues for 19 divergent accessions (see Experimental procedures and Gan et al., 2011), Arabidopsis HULK genes are expressed in all plant organs, as well as both vegetative and reproductive shoots
Jali SS, Rosloski SM, Janakirama P, Steffen JG, Zhurov V, Berleth T, Clark RM, Grbic V - A plant-specific HUA2-LIKE (HULK) gene family in Arabidopsis thaliana is essential for development
Visit the PubMed ArticleBased on both AtGenExpress (Schmid et al., 2005) and deep RNA-Seq read data from various tissues for 19 divergent accessions (see Experimental procedures and Gan et al., 2011), Arabidopsis HULK genes are expressed in all plant organs, as well as both vegetative and reproductive shoots (Figure S3). Although relative expression levels varied modestly by tissue ... HULK1 having the lowest expression
Jali SS, Rosloski SM, Janakirama P, Steffen JG, Zhurov V, Berleth T, Clark RM, Grbic V - A plant-specific HUA2-LIKE (HULK) gene family in Arabidopsis thaliana is essential for development
Visit the PubMed Articlewe performed in situ hybridization of both vegetative and reproductive apices of Arabidopsis ... we observed that HUA2 was expressed throughout ... vegetative and reproductive apices
Jali SS, Rosloski SM, Janakirama P, Steffen JG, Zhurov V, Berleth T, Clark RM, Grbic V - A plant-specific HUA2-LIKE (HULK) gene family in Arabidopsis thaliana is essential for development
Visit the PubMed ArticleHULK2 (AT2G48160) ... The presence of putative NLS motifs in each of the HULK proteins indicates that they may localize in the nucleus. To assess the subcellular protein localization of HULK gene products, we transiently expressed and visualized GFP/YFP-tagged proteins in tobacco epidermal cells (Nicotiana benthamiana). All four HULK proteins localized to the nucleus
Jali SS, Rosloski SM, Janakirama P, Steffen JG, Zhurov V, Berleth T, Clark RM, Grbic V - A plant-specific HUA2-LIKE (HULK) gene family in Arabidopsis thaliana is essential for development
Visit the PubMed Articlehua2–7 hulk1 ... several other genes with known roles in flowering time regulation displayed decreased expression levels, such as ... TEM1
Jali SS, Rosloski SM, Janakirama P, Steffen JG, Zhurov V, Berleth T, Clark RM, Grbic V - A plant-specific HUA2-LIKE (HULK) gene family in Arabidopsis thaliana is essential for development
Visit the PubMed ArticleBased on both AtGenExpress (Schmid et al., 2005) and deep RNA-Seq read data from various tissues for 19 divergent accessions (see Experimental procedures and Gan et al., 2011), Arabidopsis HULK genes are expressed in all plant organs, as well as both vegetative and reproductive shoots (Figure S3). Although relative expression levels varied modestly by tissue, HULK2 ... typically expressed more highly
Jali SS, Rosloski SM, Janakirama P, Steffen JG, Zhurov V, Berleth T, Clark RM, Grbic V - A plant-specific HUA2-LIKE (HULK) gene family in Arabidopsis thaliana is essential for development
Visit the PubMed Articlehua2–7 hulk1 ... several other genes with known roles in flowering time regulation displayed decreased expression levels, such as ... TEM2
Jali SS, Rosloski SM, Janakirama P, Steffen JG, Zhurov V, Berleth T, Clark RM, Grbic V - A plant-specific HUA2-LIKE (HULK) gene family in Arabidopsis thaliana is essential for development
Visit the PubMed Articlehua2–7 ... several other genes with known roles in flowering time regulation displayed decreased expression levels, such as ... TEM2
Jali SS, Rosloski SM, Janakirama P, Steffen JG, Zhurov V, Berleth T, Clark RM, Grbic V - A plant-specific HUA2-LIKE (HULK) gene family in Arabidopsis thaliana is essential for development
Visit the PubMed Articlehua2–7 ... FLC is down-regulated
Jali SS, Rosloski SM, Janakirama P, Steffen JG, Zhurov V, Berleth T, Clark RM, Grbic V - A plant-specific HUA2-LIKE (HULK) gene family in Arabidopsis thaliana is essential for development
Visit the PubMed ArticleHULK3 (AT3G63070) ... The presence of putative NLS motifs in each of the HULK proteins indicates that they may localize in the nucleus. To assess the subcellular protein localization of HULK gene products, we transiently expressed and visualized GFP/YFP-tagged proteins in tobacco epidermal cells (Nicotiana benthamiana). All four HULK proteins localized to the nucleus
Jali SS, Rosloski SM, Janakirama P, Steffen JG, Zhurov V, Berleth T, Clark RM, Grbic V - A plant-specific HUA2-LIKE (HULK) gene family in Arabidopsis thaliana is essential for development
Visit the PubMed ArticleBased on both AtGenExpress (Schmid et al., 2005) and deep RNA-Seq read data from various tissues for 19 divergent accessions (see Experimental procedures and Gan et al., 2011), Arabidopsis HULK genes are expressed in all plant organs, as well as both vegetative and reproductive shoots (Figure S3). Although relative expression levels varied modestly by tissue ... HULK3 ... typically expressed more highly
Jali SS, Rosloski SM, Janakirama P, Steffen JG, Zhurov V, Berleth T, Clark RM, Grbic V - A plant-specific HUA2-LIKE (HULK) gene family in Arabidopsis thaliana is essential for development
Visit the PubMed ArticleHULK1 (AT5G08230) ... The presence of putative NLS motifs in each of the HULK proteins indicates that they may localize in the nucleus. To assess the subcellular protein localization of HULK gene products, we transiently expressed and visualized GFP/YFP-tagged proteins in tobacco epidermal cells (Nicotiana benthamiana). All four HULK proteins localized to the nucleus
Jali SS, Rosloski SM, Janakirama P, Steffen JG, Zhurov V, Berleth T, Clark RM, Grbic V - A plant-specific HUA2-LIKE (HULK) gene family in Arabidopsis thaliana is essential for development
Visit the PubMed ArticleHULK2 ... was expressed broadly in vegetative and reproductive apices in domains that are essentially indistinguishable from those for HUA2
Jali SS, Rosloski SM, Janakirama P, Steffen JG, Zhurov V, Berleth T, Clark RM, Grbic V - A plant-specific HUA2-LIKE (HULK) gene family in Arabidopsis thaliana is essential for development
Visit the PubMed Articlehua2–7 hulk1 hulk2 ... several other genes with known roles in flowering time regulation displayed decreased expression levels, such as ... TEM2
Jali SS, Rosloski SM, Janakirama P, Steffen JG, Zhurov V, Berleth T, Clark RM, Grbic V - A plant-specific HUA2-LIKE (HULK) gene family in Arabidopsis thaliana is essential for development
Visit the PubMed Articlehua2–7 hulk1 hulk2 ... several other genes with known roles in flowering time regulation displayed decreased expression levels, such as ... TEM1
Jali SS, Rosloski SM, Janakirama P, Steffen JG, Zhurov V, Berleth T, Clark RM, Grbic V - A plant-specific HUA2-LIKE (HULK) gene family in Arabidopsis thaliana is essential for development
Visit the PubMed Articlehua2–7 hulk1 hulk2 ... several other genes with known roles in flowering time regulation displayed decreased expression levels, such as MAF1
Jali SS, Rosloski SM, Janakirama P, Steffen JG, Zhurov V, Berleth T, Clark RM, Grbic V - A plant-specific HUA2-LIKE (HULK) gene family in Arabidopsis thaliana is essential for development
Visit the PubMed Articlewe performed in situ hybridization of both vegetative and reproductive apices of Arabidopsis ... we observed that HUA2 was expressed throughout young primordia
Jali SS, Rosloski SM, Janakirama P, Steffen JG, Zhurov V, Berleth T, Clark RM, Grbic V - A plant-specific HUA2-LIKE (HULK) gene family in Arabidopsis thaliana is essential for development
Visit the PubMed ArticleHUA2 (AT5G23150) ... The presence of putative NLS motifs in each of the HULK proteins indicates that they may localize in the nucleus. To assess the subcellular protein localization of HULK gene products, we transiently expressed and visualized GFP/YFP-tagged proteins in tobacco epidermal cells (Nicotiana benthamiana). All four HULK proteins localized to the nucleus
Jali SS, Rosloski SM, Janakirama P, Steffen JG, Zhurov V, Berleth T, Clark RM, Grbic V - A plant-specific HUA2-LIKE (HULK) gene family in Arabidopsis thaliana is essential for development
Visit the PubMed Articlehua2–7 hulk1 hulk2 ... FLC is down-regulated
Jali SS, Rosloski SM, Janakirama P, Steffen JG, Zhurov V, Berleth T, Clark RM, Grbic V - A plant-specific HUA2-LIKE (HULK) gene family in Arabidopsis thaliana is essential for development
Visit the PubMed Articlewe performed in situ hybridization of both vegetative and reproductive apices of Arabidopsis ... HULK1 ... was expressed broadly in vegetative and reproductive apices in domains that are essentially indistinguishable from those for HUA2
Jali SS, Rosloski SM, Janakirama P, Steffen JG, Zhurov V, Berleth T, Clark RM, Grbic V - A plant-specific HUA2-LIKE (HULK) gene family in Arabidopsis thaliana is essential for development
Visit the PubMed ArticleAccording to public microarray data, VCC mRNA seems to be more abundant in roots (phloem), hypocotyl, shoot apex, flowers, and embryos during seed development (eFP Browser; http://bar.utoronto.ca; Supplemental Figs. S4 and S5). To corroborate these data, we performed quantitative RT-PCR analysis using RNA from different tissues/developmental stages. Our results confirm that the VCC transcript is most abundant in 2-month-old hypocotyls, although it is detected in other organs/developmental stages
Roschzttardtz H, Paez-Valencia J, Dittakavi T, Jali S, Reyes FC, Baisa G, Anne P, Gissot L, Palauqui JC, Masson PH, Bednarek SY, Otegui MS - The VASCULATURE COMPLEXITY AND CONNECTIVITY gene encodes a plant-specific protein required for embryo provasculature development
Visit the PubMed ArticleTwenty pVCC:GUS transgenic lines were isolated and analyzed. GUS activity was detected in ... cotyledon veins after germination
Roschzttardtz H, Paez-Valencia J, Dittakavi T, Jali S, Reyes FC, Baisa G, Anne P, Gissot L, Palauqui JC, Masson PH, Bednarek SY, Otegui MS - The VASCULATURE COMPLEXITY AND CONNECTIVITY gene encodes a plant-specific protein required for embryo provasculature development
Visit the PubMed ArticleWe also analyzed lines expressing pPIN1:PIN1-GFP and did not detect any changes in either the expression or the polar localization of PIN1-GFP in vcc-2 embryos
Roschzttardtz H, Paez-Valencia J, Dittakavi T, Jali S, Reyes FC, Baisa G, Anne P, Gissot L, Palauqui JC, Masson PH, Bednarek SY, Otegui MS - The VASCULATURE COMPLEXITY AND CONNECTIVITY gene encodes a plant-specific protein required for embryo provasculature development
Visit the PubMed ArticleWe did not detect changes in DR5rev promoter activity caused by the vcc-2 mutation
Roschzttardtz H, Paez-Valencia J, Dittakavi T, Jali S, Reyes FC, Baisa G, Anne P, Gissot L, Palauqui JC, Masson PH, Bednarek SY, Otegui MS - The VASCULATURE COMPLEXITY AND CONNECTIVITY gene encodes a plant-specific protein required for embryo provasculature development
Visit the PubMed ArticleTo analyze in more detail the expression pattern of VCC, we obtained transgenic plants expressing GUS under the transcriptional control of a 1,900-bp DNA fragment derived from a sequence located immediately upstream of the VCC coding region. Twenty pVCC:GUS transgenic lines were isolated and analyzed. GUS activity was detected in procambial cells at the hypocotyl of mature embryos
Roschzttardtz H, Paez-Valencia J, Dittakavi T, Jali S, Reyes FC, Baisa G, Anne P, Gissot L, Palauqui JC, Masson PH, Bednarek SY, Otegui MS - The VASCULATURE COMPLEXITY AND CONNECTIVITY gene encodes a plant-specific protein required for embryo provasculature development
Visit the PubMed ArticleSince we failed to detect consistent GUS expression in developing embryos, we performed RT-PCR and in situ hybridization of VCC transcripts and confirmed that VCC is expressed in torpedo and bent cotyledon stage embryos (Fig. 5, H and I). The detection of VCC transcripts by in situ hybridization showed that VCC is expressed in all embryo cells, including procambial cells, at the bent cotyledon embryo stage
Roschzttardtz H, Paez-Valencia J, Dittakavi T, Jali S, Reyes FC, Baisa G, Anne P, Gissot L, Palauqui JC, Masson PH, Bednarek SY, Otegui MS - The VASCULATURE COMPLEXITY AND CONNECTIVITY gene encodes a plant-specific protein required for embryo provasculature development
Visit the PubMed ArticleNext, we tested whether the epidermal- specific expression of ABS5/T5L1 is sufficient to alter auxin homeostasis. We transformed DR5::GUS plants with PAtML1::ABS5 construct and assayed GUS activities in independent transgenic lines that exhibited the upward curling leaf phenotype. Figure 8D-F showed that auxin distributions as indicated by the expressions of DR5::GUS were increased in PAtML1::ABS5 lines in a way that is similar to what was found in abs5-1D or the ABS5/T5L1 OE lines
An R, Liu X, Wang R, Wu H, Liang S, Shao J, Qi Y, An L, Yu F - The over-expression of two transcription factors, ABS5/bHLH30 and ABS7/MYB101, leads to upwardly curly leaves
Visit the PubMed ArticleWe compared the accumulation of ... ICU3 ... transcripts in ... activation-tagged lines of ... ABS7/MYB101 using semi-quantitative RT-PCR. No significant changes in the expression levels ... were observed
An R, Liu X, Wang R, Wu H, Liang S, Shao J, Qi Y, An L, Yu F - The over-expression of two transcription factors, ABS5/bHLH30 and ABS7/MYB101, leads to upwardly curly leaves
Visit the PubMed ArticleWe compared the accumulation of ... ICU3 ... transcripts in ... activation-tagged lines of ABS5/T5L1 ... using semi-quantitative RT-PCR. No significant changes in the expression levels ... were observed
An R, Liu X, Wang R, Wu H, Liang S, Shao J, Qi Y, An L, Yu F - The over-expression of two transcription factors, ABS5/bHLH30 and ABS7/MYB101, leads to upwardly curly leaves
Visit the PubMed ArticleWe compared the accumulation of ... ICU2 ... transcripts in ... activation-tagged lines of ... ABS7/MYB101 using semi-quantitative RT-PCR. No significant changes in the expression levels ... were observed
An R, Liu X, Wang R, Wu H, Liang S, Shao J, Qi Y, An L, Yu F - The over-expression of two transcription factors, ABS5/bHLH30 and ABS7/MYB101, leads to upwardly curly leaves
Visit the PubMed ArticleWe compared the accumulation of ICU1 ... transcripts in ... activation-tagged lines of ABS5/T5L1 ... using semi-quantitative RT-PCR. No significant changes in the expression levels ... were observed
An R, Liu X, Wang R, Wu H, Liang S, Shao J, Qi Y, An L, Yu F - The over-expression of two transcription factors, ABS5/bHLH30 and ABS7/MYB101, leads to upwardly curly leaves
Visit the PubMed ArticleAs an initial attempt to understand the function of ABS5/T5L1, we examined its tissue expression profile via semi-quantitative RT-PCR with cDNAs obtained from various wild type Arabidopsis tissues. Figure 3A shows that ABS5 transcripts accumulated in all tissues examined. ABS5/T5L1 expression was relatively lower in aerial part of two-week-old seedlings and older rosette leaves but is highly expressed in roots and stems
An R, Liu X, Wang R, Wu H, Liang S, Shao J, Qi Y, An L, Yu F - The over-expression of two transcription factors, ABS5/bHLH30 and ABS7/MYB101, leads to upwardly curly leaves
Visit the PubMed ArticleWe compared the accumulation of ... ICU3 ... transcripts in ... loss-of-function ... lines of ABS5/T5L1 ... using semi-quantitative RT-PCR. No significant changes in the expression levels ... were observed
An R, Liu X, Wang R, Wu H, Liang S, Shao J, Qi Y, An L, Yu F - The over-expression of two transcription factors, ABS5/bHLH30 and ABS7/MYB101, leads to upwardly curly leaves
Visit the PubMed ArticleWe compared the accumulation of ... ICU2 ... transcripts in ... loss-of-function ... lines of ... ABS7/MYB101 using semi-quantitative RT-PCR. No significant changes in the expression levels ... were observed
An R, Liu X, Wang R, Wu H, Liang S, Shao J, Qi Y, An L, Yu F - The over-expression of two transcription factors, ABS5/bHLH30 and ABS7/MYB101, leads to upwardly curly leaves
Visit the PubMed ArticleWe compared the accumulation of ... ICU4 transcripts in ... activation-tagged lines of ABS5/T5L1 ... using semi-quantitative RT-PCR. No significant changes in the expression levels ... were observed
An R, Liu X, Wang R, Wu H, Liang S, Shao J, Qi Y, An L, Yu F - The over-expression of two transcription factors, ABS5/bHLH30 and ABS7/MYB101, leads to upwardly curly leaves
Visit the PubMed ArticleWe compared the accumulation of ... ICU4 transcripts in ... loss-of-function ... lines of ... ABS7/MYB101 using semi-quantitative RT-PCR. No significant changes in the expression levels ... were observed
An R, Liu X, Wang R, Wu H, Liang S, Shao J, Qi Y, An L, Yu F - The over-expression of two transcription factors, ABS5/bHLH30 and ABS7/MYB101, leads to upwardly curly leaves
Visit the PubMed ArticleWe compared the accumulation of ... ICU2 ... transcripts in ... loss-of-function ... lines of ABS5/T5L1 ... using semi-quantitative RT-PCR. No significant changes in the expression levels ... were observed
An R, Liu X, Wang R, Wu H, Liang S, Shao J, Qi Y, An L, Yu F - The over-expression of two transcription factors, ABS5/bHLH30 and ABS7/MYB101, leads to upwardly curly leaves
Visit the PubMed ArticleThe expression patterns of the synthetic DR5::GUS reporter gene were used to deduce the distributions of auxin maxima [34]. In wild type background, the strongest DR5::GUS signals coincided with the positions of the hydathodes in cotyledons and the first true leaves of two-week-old seedlings (Figure 4A–B). However, in abs5-1D/+ heterozygous background, DR5::GUS activities were less restricted but more evenly distributed along the entire leaf margin compared to that of wild type (Figure 4C–D). As illustrated in Figure 4E–G, stronger and more diffused GUS signals were also found in leaf marginal areas in transgenic lines over-expressing ABS5/T5L1 in DR5::GUS background
An R, Liu X, Wang R, Wu H, Liang S, Shao J, Qi Y, An L, Yu F - The over-expression of two transcription factors, ABS5/bHLH30 and ABS7/MYB101, leads to upwardly curly leaves
Visit the PubMed ArticleWe next analyzed the accumulation of ABS7/MYB101 transcripts in different Arabidopsis tissues. As shown in Figure 6E, ABS7/MYB101 transcripts were only detected in flowers and siliques by semi-quantitative RT-PCR. This is consistent with previous finding that ABS7/MYB101 is highly expressed in seeds and floral tissues
An R, Liu X, Wang R, Wu H, Liang S, Shao J, Qi Y, An L, Yu F - The over-expression of two transcription factors, ABS5/bHLH30 and ABS7/MYB101, leads to upwardly curly leaves
Visit the PubMed ArticleWe compared the accumulation of ... ICU4 transcripts in ... activation-tagged lines of ... ABS7/MYB101 using semi-quantitative RT-PCR. No significant changes in the expression levels ... were observed
An R, Liu X, Wang R, Wu H, Liang S, Shao J, Qi Y, An L, Yu F - The over-expression of two transcription factors, ABS5/bHLH30 and ABS7/MYB101, leads to upwardly curly leaves
Visit the PubMed ArticleWe compared the accumulation of ICU1 ... transcripts in ... loss-of-function ... lines of ... ABS7/MYB101 using semi-quantitative RT-PCR. No significant changes in the expression levels ... were observed
An R, Liu X, Wang R, Wu H, Liang S, Shao J, Qi Y, An L, Yu F - The over-expression of two transcription factors, ABS5/bHLH30 and ABS7/MYB101, leads to upwardly curly leaves
Visit the PubMed ArticleWe compared the accumulation of ICU1 ... transcripts in ... activation-tagged lines of ... ABS7/MYB101 using semi-quantitative RT-PCR. No significant changes in the expression levels ... were observed
An R, Liu X, Wang R, Wu H, Liang S, Shao J, Qi Y, An L, Yu F - The over-expression of two transcription factors, ABS5/bHLH30 and ABS7/MYB101, leads to upwardly curly leaves
Visit the PubMed ArticleWe compared the accumulation of ... ICU2 ... transcripts in ... activation-tagged lines of ABS5/T5L1 ... using semi-quantitative RT-PCR. No significant changes in the expression levels ... were observed
An R, Liu X, Wang R, Wu H, Liang S, Shao J, Qi Y, An L, Yu F - The over-expression of two transcription factors, ABS5/bHLH30 and ABS7/MYB101, leads to upwardly curly leaves
Visit the PubMed ArticleWe next explored the sub-cellular localization of ABS5/T5L1 protein. Vectors expressing eGFP alone or the ABS5-GFP fusion protein under the control of 35S promoter were used to transform wild type leaf protoplasts. Nuclei of protoplasts were labeled via staining with the fluorescent dye Hoechst33342 [33]. Protoplasts expressing the control P35S::GFP showed GFP signals in both the cytosol and the nucleus (Figure 3B). In contrast, protoplasts expressing P35S::ABS5-GFP displayed green fluorescence signals exclusively in the nucleus, as indicated by the Hoechst33342 staining (Figure 3B). These data show that ABS5/T5L1 resides in the nucleus, consistent with its potential function as a transcription factor
An R, Liu X, Wang R, Wu H, Liang S, Shao J, Qi Y, An L, Yu F - The over-expression of two transcription factors, ABS5/bHLH30 and ABS7/MYB101, leads to upwardly curly leaves
Visit the PubMed ArticleWe compared the accumulation of ... ICU3 ... transcripts in ... loss-of-function ... lines of ... ABS7/MYB101 using semi-quantitative RT-PCR. No significant changes in the expression levels ... were observed
An R, Liu X, Wang R, Wu H, Liang S, Shao J, Qi Y, An L, Yu F - The over-expression of two transcription factors, ABS5/bHLH30 and ABS7/MYB101, leads to upwardly curly leaves
Visit the PubMed ArticleConsistent with its identity as a transcription factor, ABS7-GFP localized to the nucleus in protoplast transient expression assays
An R, Liu X, Wang R, Wu H, Liang S, Shao J, Qi Y, An L, Yu F - The over-expression of two transcription factors, ABS5/bHLH30 and ABS7/MYB101, leads to upwardly curly leaves
Visit the PubMed ArticleWe compared the accumulation of ICU1 ... transcripts in ... loss-of-function ... lines of ABS5/T5L1 ... using semi-quantitative RT-PCR. No significant changes in the expression levels ... were observed
An R, Liu X, Wang R, Wu H, Liang S, Shao J, Qi Y, An L, Yu F - The over-expression of two transcription factors, ABS5/bHLH30 and ABS7/MYB101, leads to upwardly curly leaves
Visit the PubMed ArticleWe compared the accumulation of ... ICU4 transcripts in ... loss-of-function ... lines of ABS5/T5L1 ... using semi-quantitative RT-PCR. No significant changes in the expression levels ... were observed
An R, Liu X, Wang R, Wu H, Liang S, Shao J, Qi Y, An L, Yu F - The over-expression of two transcription factors, ABS5/bHLH30 and ABS7/MYB101, leads to upwardly curly leaves
Visit the PubMed ArticleTwo existing orthologues of LSG1 in A. thaliana prompted the question whether the two genes show a differential expression. To this end, the expression level of LSG1-1 and LSG1-2 in different tissues and developmental stages was determined by quantitative RT-PCR (qRT-PCR, Figure 5a). The expression was normalized to the amount of RNA used for reverse transcription because the expression of reference genes like UBIQUITIN3 (UBI3) is not equal in all samples (Missbach et al., 2013). Both mRNAs can be detected in all tissues and developmental stages tested, suggesting that both proteins are ubiquitously expressed (Figure 5a). Interestingly, LSG1-2 is highly expressed in reproductive and strongly dividing tissues like young plants, flowers and roots (Figure 5a). For these tissues, a high amount of synthesized ribosomes is required to ensure fast growth. Furthermore, this expression profile parallels that for other ribosome biogenesis co-factors
Weis BL, Missbach S, Marzi J, Bohnsack MT, Schleiff E - The 60S associated ribosome biogenesis factor LSG1-2 is required for 40S maturation in Arabidopsis thaliana
Visit the PubMed ArticleTo support a functional relation of atLSG1-1 ... in ribosome biogenesis, we applied A. thaliana cell culture lysate to continuous sucrose density gradient centrifugation. The UV absorption profile clearly shows peaks for 40S, 60S/80S and polysomes, which is confirmed by the mature rRNAs in the corresponding fractions (Figure 3). By western blotting with antibodies against atNOB1 and atENP1 we confirmed that pre-40S migrates in fractions 11 and 12, while pre-60S could be identified in fractions 15 and 16 by the presence of the RBF ateIF6-2 and the 27SB pre-rRNA (Figure 3; Missbach et al., 2013). Western blot analysis using the atLSG1 antibody demonstrates that ... atLSG1-1 is exclusively found at the top part of the gradient where free proteins and RNAs reside
Weis BL, Missbach S, Marzi J, Bohnsack MT, Schleiff E - The 60S associated ribosome biogenesis factor LSG1-2 is required for 40S maturation in Arabidopsis thaliana
Visit the PubMed ArticleNormalized to atLSG1-1, atLSG1-2 is strongly expressed in young plants, flowers and cell culture, but less in rosette, stem and hypsophyll that do not have a high demand for newly synthesized ribosomes
Weis BL, Missbach S, Marzi J, Bohnsack MT, Schleiff E - The 60S associated ribosome biogenesis factor LSG1-2 is required for 40S maturation in Arabidopsis thaliana
Visit the PubMed Articlewe analysed the subcellular localization ... in A. thaliana ... We generated GFP fusions for expression in A. thaliana mesophyll protoplasts co-transformed with a nucleolar marker (atFIB2-mCherry). Consistent with the cytoplasmic function of scLsg1 (Kallstrom et al., 2003; Hedges et al., 2005) we observed clear cytoplasmic fluorescence for ... atLSG1-1
Weis BL, Missbach S, Marzi J, Bohnsack MT, Schleiff E - The 60S associated ribosome biogenesis factor LSG1-2 is required for 40S maturation in Arabidopsis thaliana
Visit the PubMed Articlewe analysed the subcellular localization ... in A. thaliana. We generated GFP fusions for expression in A. thaliana mesophyll protoplasts co-transformed with a nucleolar marker (atFIB2-mCherry). Consistent with the cytoplasmic function of scLsg1 (Kallstrom et al., 2003; Hedges et al., 2005) we observed clear cytoplasmic fluorescence for ... atLSG1-2
Weis BL, Missbach S, Marzi J, Bohnsack MT, Schleiff E - The 60S associated ribosome biogenesis factor LSG1-2 is required for 40S maturation in Arabidopsis thaliana
Visit the PubMed ArticleWe generated GFP fusions for expression in A. thaliana mesophyll protoplasts ... the GFP fluorescence was also obtained in the nucleus in case of atLSG1-2–GFP
Weis BL, Missbach S, Marzi J, Bohnsack MT, Schleiff E - The 60S associated ribosome biogenesis factor LSG1-2 is required for 40S maturation in Arabidopsis thaliana
Visit the PubMed ArticleTwo existing orthologues of LSG1 in A. thaliana prompted the question whether the two genes show a differential expression. To this end, the expression level of LSG1-1 and LSG1-2 in different tissues and developmental stages was determined by quantitative RT-PCR (qRT-PCR, Figure 5a). The expression was normalized to the amount of RNA used for reverse transcription because the expression of reference genes like UBIQUITIN3 (UBI3) is not equal in all samples (Missbach et al., 2013). Both mRNAs can be detected in all tissues and developmental stages tested, suggesting that both proteins are ubiquitously expressed ... expression of LSG1-1 is more equally distributed but expressed highest in stem and hypsophyll on day 66 (Figure 5a). In here, hypsophyll refers to leaf-like structures out of the axil of which a stalk of a flower arises. A similar observation can be made for the protein level of atLSG1
Weis BL, Missbach S, Marzi J, Bohnsack MT, Schleiff E - The 60S associated ribosome biogenesis factor LSG1-2 is required for 40S maturation in Arabidopsis thaliana
Visit the PubMed Articleimgi2spy4 mutants ... decrease in the expression of the cytokinin response genes ARR4 to ARR7
Putarjunan A, Rodermel S - gigantea suppresses immutans variegation by interactions with cytokinin and gibberellin signaling pathways
Visit the PubMed Articleimgi2spy4 mutants ... marked increase in the expression of GASA4, a representative GA response gene
Putarjunan A, Rodermel S - gigantea suppresses immutans variegation by interactions with cytokinin and gibberellin signaling pathways
Visit the PubMed ArticleWe next conducted western immunoblot analyses to examine the accumulation of seven representative photosynthetic proteins from im, gi2, imgi2, and Col-0 leaves at developmental stages I to III; the proteins were loaded on the gels on an equal fresh weight basis. Figure 7 shows that, during stage I, each of the seven proteins accumulates to a similar extent in gi2 and the wild type and also to a similar, although reduced, extent in im and imgi2; the decreases in im and imgi2 are expected and caused by the presence of white tissues in the whole-leaf samples (Aluru et al., 2007). During stage II, the pattern of protein accumulation in imgi2 begins to resemble that of gi2 (a notable exception being D1), such that by stage III, all seven proteins have accumulated to high levels and the imgi2 and gi2 profiles are indistinguishable. Stage III wild-type and im leaves, on the other hand, are senescent and, not surprisingly, have much reduced amounts of all seven proteins
Putarjunan A, Rodermel S - gigantea suppresses immutans variegation by interactions with cytokinin and gibberellin signaling pathways
Visit the PubMed ArticleARR4–ARR7 ... In addition to an elevated expression in BAP-treated im (Fig. 3C), ARRs are dramatically up-regulated in stage II and III imgi2 leaves
Putarjunan A, Rodermel S - gigantea suppresses immutans variegation by interactions with cytokinin and gibberellin signaling pathways
Visit the PubMed Articlein BAP-treated ... imgi2 leaves ... this induction is not accompanied by enhanced expression of ... cytokinin biosynthetic ... IPT5
Putarjunan A, Rodermel S - gigantea suppresses immutans variegation by interactions with cytokinin and gibberellin signaling pathways
Visit the PubMed Articlein BAP-treated ... imgi2 leaves ... this induction is not accompanied by enhanced expression of ... cytokinin biosynthetic ... IPT1
Putarjunan A, Rodermel S - gigantea suppresses immutans variegation by interactions with cytokinin and gibberellin signaling pathways
Visit the PubMed Articleimgi2spy4 mutants ... decrease in the expression of the cytokinin response genes ARR4 to ARR7
Putarjunan A, Rodermel S - gigantea suppresses immutans variegation by interactions with cytokinin and gibberellin signaling pathways
Visit the PubMed ArticleWe suggest that a similar cytokinin-dependent mechanism is operative in imgi2, inasmuch as the alterations in chloroplast number and size in imgi2 are accompanied by a sharp up-regulation o ... PDV2 expression ... Importantly, this up-regulation occurs late in development, coincident with the time when gi-mediated effects on cytokinin synthesis/signaling are proposed to be triggered and newly emerging im leaves become all green
Putarjunan A, Rodermel S - gigantea suppresses immutans variegation by interactions with cytokinin and gibberellin signaling pathways
Visit the PubMed Articleimgi2spy4 mutants ... decrease in the expression of the cytokinin response genes ARR4 to ARR7
Putarjunan A, Rodermel S - gigantea suppresses immutans variegation by interactions with cytokinin and gibberellin signaling pathways
Visit the PubMed ArticleARR4–ARR7 ... In addition to an elevated expression in BAP-treated im (Fig. 3C), ARRs are dramatically up-regulated in stage II and III imgi2 leaves
Putarjunan A, Rodermel S - gigantea suppresses immutans variegation by interactions with cytokinin and gibberellin signaling pathways
Visit the PubMed Articleimgi2spy4 mutants ... decrease in the expression of the cytokinin response genes ARR4 to ARR7
Putarjunan A, Rodermel S - gigantea suppresses immutans variegation by interactions with cytokinin and gibberellin signaling pathways
Visit the PubMed ArticleARR4–ARR7 ... In addition to an elevated expression in BAP-treated im (Fig. 3C), ARRs are dramatically up-regulated in stage II and III imgi2 leaves
Putarjunan A, Rodermel S - gigantea suppresses immutans variegation by interactions with cytokinin and gibberellin signaling pathways
Visit the PubMed ArticleWe suggest that a similar cytokinin-dependent mechanism is operative in imgi2, inasmuch as the alterations in chloroplast number and size in imgi2 are accompanied by a sharp up-regulation of CRF2 ... expression ... Importantly, this up-regulation occurs late in development, coincident with the time when gi-mediated effects on cytokinin synthesis/signaling are proposed to be triggered and newly emerging im leaves become all green
Putarjunan A, Rodermel S - gigantea suppresses immutans variegation by interactions with cytokinin and gibberellin signaling pathways
Visit the PubMed ArticleARR4–ARR7 ... In addition to an elevated expression in BAP-treated im (Fig. 3C), ARRs are dramatically up-regulated in stage II and III imgi2 leaves
Putarjunan A, Rodermel S - gigantea suppresses immutans variegation by interactions with cytokinin and gibberellin signaling pathways
Visit the PubMed ArticleCRU1 ... expressed at high levels in 6 d gct seedlings, and their expression declined gradually in this mutant over the next 2 weeks
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed Articlegct ... had no significant effect on the expression of miR159
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed Articlethe mRNA levels of ... SPL3 ... reduced in ... gct
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed Articlehighly overexpressed in ... gct ... the flowering repressor FLC
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed Articlein gct ... decrease in the expression of miR172 at 7 d, 14 d and 21 d
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed Articlein ... cct ... flowering genes that showed significant decreases in expression included ... CONSTANS (CO
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed Articlethe mRNA levels of ... SPL3 ... reduced in ... cct
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed Articlegct ... reduced expression of ... CO
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed ArticleOLEO1 ... expressed at high levels in 6 d gct seedlings, and their expression declined gradually in this mutant over the next 2 weeks ... We also compared the expression of these genes in 7 d gct and cct seedlings with the grow and arrest phenotypes (Fig. 2F). In every case, these genes were expressed at significantly higher levels in seedlings with the arrest phenotype ... OLEO1 was elevated 4-fold in grow gct seedlings
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed ArticleATTI7 ... expressed at high levels in 6 d gct seedlings, and their expression declined gradually in this mutant over the next 2 weeks
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed Articlethe mRNA levels of ... SPL9 ... reduced in ... cct ... although in the case of SPL9 this effect was only observed at 21 d
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed ArticleCRU1 was unaffected by cct
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed Articlecct ... elevated expression of the floral repressor FLC ... FLC transcripts were much higher than in wt at 6 d, and had decreased several fold by 21 d ... Although FLC is expressed more highly in juvenile than in adult leaves (supplementary material Fig. S3), a secondary effect of leaf identity is unlikely to explain the large differences in FLC levels between wt, gct and cct because the largest differences in FLC were observed at 6 d, when all three genotypes have only two juvenile leaf primordia
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed ArticleNorthern blot analysis revealed that miR156 levels were approximately twice as high in gct ... as in wt plants at 7 d, 14 d and 21 d after germination. miR156 levels decreased steadily in all genotypes, so that at 21 d the amount of miR156 was about half that seen at 7 d
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed Articlethe mRNA levels of ... SPL9 ... reduced in ... gct ... although in the case of SPL9 this effect was only observed at 21 d
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed Articlecct had no significant effect on the expression of ... miR161
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed Articlecct had no significant effect on the expression of ... miR168
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed Articlegct ... reduced expression of ... SOC1 ... SOC1 increased slightly from 6 d to 21 d ... These changes in FT and SOC1 gene expression are not attributable to differences in leaf identity between wt, gct and cct, as FT and SOC1 show essentially the same expression in juvenile and adult leaves
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed Articlegct ... had no significant effect on the expression of ... miR168
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed Articlein ... cct ... flowering genes that showed significant decreases in expression included ... SUPPRESSOR OF CONSTANS 1 (SOC1)
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed Articlehighly overexpressed in ... cct ... the flowering repressor FLC
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed Articlegct ... had no significant effect on the expression of ... miR161
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed Articlegct ... reduced expression of ... FRUITFULL
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed Articlegct ... reduced expression of ... SEPALLATA3
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed Articlein ... cct ... flowering genes that showed significant decreases in expression included FLOWERING LOCUS T (FT)
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed Articlegct ... reduced expression of ... FT ... levels of FT ... increased slightly from 6 d to 21 d ... These changes in FT and SOC1 gene expression are not attributable to differences in leaf identity between wt, gct and cct, as FT and SOC1 show essentially the same expression in juvenile and adult leaves
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed ArticleAt2S2 ... also misexpressed in 6 d cct seedlings, but were much less elevated than in gct ... also compared the expression of these genes in 7 d gct and cct seedlings with the grow and arrest phenotypes (Fig. 2F). In every case, these genes were expressed at significantly higher levels in seedlings with the arrest phenotype. For example, At2S2 was elevated ... 88-fold in arrest gct seedlings
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed ArticleATTI7 ... also misexpressed in 6 d cct seedlings, but were much less elevated than in gct
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed ArticleAt2S2 ... expressed at high levels in 6 d gct seedlings, and their expression declined gradually in this mutant over the next 2 weeks ... We also compared the expression of these genes in 7 d gct and cct seedlings with the grow and arrest phenotypes (Fig. 2F). In every case, these genes were expressed at significantly higher levels in seedlings with the arrest phenotype. For example, At2S2 was elevated 3-fold in grow gct seedlings
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed Articlein ... gct ... flowering genes that showed significant decreases in expression included FLOWERING LOCUS T (FT)
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed ArticleAnalysis of ... CCT expression in a public dataset revealed ... broadly expressed at low levels throughout post-embryonic development
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed Articlecct ... reduced expression of ... SOC1 ... SOC1 increased slightly from 6 d to 21 d ... These changes in FT and SOC1 gene expression are not attributable to differences in leaf identity between wt, gct and cct, as FT and SOC1 show essentially the same expression in juvenile and adult leaves
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed Articlegct ... elevated expression of the floral repressor FLC ... FLC transcripts were much higher than in wt at 6 d, and had decreased several fold by 21 d ... Although FLC is expressed more highly in juvenile than in adult leaves (supplementary material Fig. S3), a secondary effect of leaf identity is unlikely to explain the large differences in FLC levels between wt, gct and cct because the largest differences in FLC were observed at 6 d, when all three genotypes have only two juvenile leaf primordia
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed ArticleNorthern blot analysis revealed that miR156 levels were approximately twice as high in ... cct mutants as in wt plants at 7 d, 14 d and 21 d after germination. miR156 levels decreased steadily in all genotypes, so that at 21 d the amount of miR156 was about half that seen at 7 d
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed ArticleAnalysis of GCT ... expression in a public dataset revealed ... broadly expressed at low levels throughout post-embryonic development
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed Articlecct ... reduced expression of ... FT ... levels of FT ... increased slightly from 6 d to 21 d ... These changes in FT and SOC1 gene expression are not attributable to differences in leaf identity between wt, gct and cct, as FT and SOC1 show essentially the same expression in juvenile and adult leaves
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed Articlecct had no significant effect on the expression of miR159
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed Articlecct ... reduced expression of ... SEPALLATA3
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed Articlein ... gct ... flowering genes that showed significant decreases in expression included ... SUPPRESSOR OF CONSTANS 1 (SOC1)
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed Articlewe examined FLC levels in 35S::miR156A plants and found a ∼30% decrease in the level of FLC compared with wt
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed ArticleOLEO1 ... also misexpressed in 6 d cct seedlings, but were much less elevated than in gct ... We also compared the expression of these genes in 7 d gct and cct seedlings with the grow and arrest phenotypes (Fig. 2F). In every case, these genes were expressed at significantly higher levels in seedlings with the arrest phenotype ... OLEO1 was elevated ... 162-fold in arrest gct seedlings
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed Articlein ... cct ... decrease in the expression of miR172 at 7 d, 14 d and 21 d
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed Articlecct ... reduced expression of ... CO
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed Articlecct ... reduced expression of ... FRUITFULL
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed Articlein ... gct ... flowering genes that showed significant decreases in expression included ... CONSTANS (CO)
Gillmor CS, Silva-Ortega CO, Willmann MR, Buendía-Monreal M, Poethig RS - The Arabidopsis Mediator CDK8 module genes CCT (MED12) and GCT (MED13) are global regulators of developmental phase transitions
Visit the PubMed Articlein mterf9 ... class III genes rps18 ... 2.2 ... fold upregulated
Robles P, Micol JL, Quesada V - Mutations in the plant-conserved MTERF9 alter chloroplast gene expression, development and tolerance to abiotic stress in Arabidopsis thaliana
Visit the PubMed ArticleWe found that MDA1 transcript levels were also significantly higher in twr-1 than in Col-0 (1.5 ± 0.1; P = 1.5E-04)
Robles P, Micol JL, Quesada V - Mutations in the plant-conserved MTERF9 alter chloroplast gene expression, development and tolerance to abiotic stress in Arabidopsis thaliana
Visit the PubMed Articlein mterf9 ... class III genes ... rpoB ... 4.7-fold upregulated
Robles P, Micol JL, Quesada V - Mutations in the plant-conserved MTERF9 alter chloroplast gene expression, development and tolerance to abiotic stress in Arabidopsis thaliana
Visit the PubMed ArticleRBCS1A was downregulated (1.6-fold ... in the mterf9 mutant as compared with Col-0
Robles P, Micol JL, Quesada V - Mutations in the plant-conserved MTERF9 alter chloroplast gene expression, development and tolerance to abiotic stress in Arabidopsis thaliana
Visit the PubMed Articlethe amount of transcripts of class I ... psbA ... was significantly smaller in mterf9 than in the WT
Robles P, Micol JL, Quesada V - Mutations in the plant-conserved MTERF9 alter chloroplast gene expression, development and tolerance to abiotic stress in Arabidopsis thaliana
Visit the PubMed ArticleRpoTp/SCA3 was upregulated (2.6-fold) in the mterf9 mutant as compared with Col-0
Robles P, Micol JL, Quesada V - Mutations in the plant-conserved MTERF9 alter chloroplast gene expression, development and tolerance to abiotic stress in Arabidopsis thaliana
Visit the PubMed ArticleWe investigated the spatial expression pattern of the MTERF9 gene using qRT-PCR and RNA extracted from different organs. MTERF9 transcripts were detected in all the organs studied. In comparison to roots, MTERF9 was expressed at higher levels in buds, open flowers, stems and rosette leaves [upregulated 4.6-fold (4.6 ± 1.1; P < 0.01), 4.2-fold (4.2 ± 1.2; P < 0.01), 3.3-fold (3.3 ± 1.0; P < 0.01) and 2.9-fold (2.9 ± 0.5; P < 0.01), respectively]. We confirmed these experimental results through the in silico analysis of MTERF9 expression using the BAR (http://bar.utoronto.ca/efp/cgi-bin/efpWeb.cgi). The online data available from the ATH1 microarray and the At-TAX whole genome tilling array show that MTERF9 is predominantly expressed in the aerial part of seedlings, cotyledons and expanding first leaves, and that it reaches its lowest level of expression in roots.
Robles P, Micol JL, Quesada V - Mutations in the plant-conserved MTERF9 alter chloroplast gene expression, development and tolerance to abiotic stress in Arabidopsis thaliana
Visit the PubMed ArticleThe amount of transcripts of the BSM/RUG2 gene were also significantly increased in mterf9
Robles P, Micol JL, Quesada V - Mutations in the plant-conserved MTERF9 alter chloroplast gene expression, development and tolerance to abiotic stress in Arabidopsis thaliana
Visit the PubMed ArticleTo shed light on the function of MTERF9, we used the Co-Expression tool from genevestigator (https://www.genevestigator.com/gv/plant.jsp; Zimmermann et al. 2004) to find genes co-expressed (candidates to be co-regulated) with MTERF9. We extended the analysis to the mTERF genes MDA1 and BSM/RUG2 given the similarity between both the phenotypes of mterf9, mda1 and rug2-2 and the functional relationship between MDA1 and MTERF9 (see later). A list of the top 50 most correlated genes was obtained for each target gene using the dataset of anatomical parts. The Pearson's correlation coefficient (PCC) values obtained ranged from 0.73 to 0.94 (Table S5). MDA1 and MTERF9 were co-expressed (PCC = 0.82; Table S5). We analyzed the genes co-expressed with MDA1 and MTERF9 by using the GO Annotation tool from TAIR (http://www.arabidopsis.org/tools/bulk/go/index.jsp). As regards the GO terms for ‘cellular components’, the main categories for the MTERF9 co-expressed genes were ‘chloroplast’ and ‘plastid’, associated with 37 and 12 genes, respectively, representing in both cases almost a fivefold enrichment compared with the whole A. thaliana genome (Table S6). Regarding the GO terms for ‘biological processes’, the two most represented categories among the MTERF9 co-expressed genes were ‘other cellular processes’ and ‘other metabolic processes’. Similar results were obtained for MDA1 (Table S6). Twenty-one genes (43.7%) were commonly co-expressed with MDA1 and MTERF9 (Table S5), most of them (71.4%) included in the cellular component category of ‘chloroplast’ (Table S6) such as At2g36000, At4g20130, At3g20230 and At2g33430, which encode an mTERF, the PTAC14 protein, the L18p/L5e ribosomal protein and the multiple organellar RNA editing factor 2 (MORF2), respectively, all involved in plastid gene expression (Table S5). Regarding RUG2, 13 (24%) and 9 (18%) of the top 50 co-expressed genes were common to those of MDA1 and MTERF9, respectively (Table S5).
Robles P, Micol JL, Quesada V - Mutations in the plant-conserved MTERF9 alter chloroplast gene expression, development and tolerance to abiotic stress in Arabidopsis thaliana
Visit the PubMed Articlethe amount of transcripts of class I ... psbD was significantly smaller in mterf9 than in the WT
Robles P, Micol JL, Quesada V - Mutations in the plant-conserved MTERF9 alter chloroplast gene expression, development and tolerance to abiotic stress in Arabidopsis thaliana
Visit the PubMed Articlein mterf9 ... the transcript contents of the class II gene clpP (2.5-fold upregulated)
Robles P, Micol JL, Quesada V - Mutations in the plant-conserved MTERF9 alter chloroplast gene expression, development and tolerance to abiotic stress in Arabidopsis thaliana
Visit the PubMed Articlein mterf9 ... class III genes ... accD ... 2.6 ... fold upregulated
Robles P, Micol JL, Quesada V - Mutations in the plant-conserved MTERF9 alter chloroplast gene expression, development and tolerance to abiotic stress in Arabidopsis thaliana
Visit the PubMed ArticleCompared with Col-0, MDA1 was upregulated in mterf9 (1.8-fold, Table 1)
Robles P, Micol JL, Quesada V - Mutations in the plant-conserved MTERF9 alter chloroplast gene expression, development and tolerance to abiotic stress in Arabidopsis thaliana
Visit the PubMed ArticleMTERF9 was upregulated in mda1-2 (1.4 ± 0.3; P = 0.09)
Robles P, Micol JL, Quesada V - Mutations in the plant-conserved MTERF9 alter chloroplast gene expression, development and tolerance to abiotic stress in Arabidopsis thaliana
Visit the PubMed ArticleIn genetic backgrounds with reduced REV mRNA ... 35S::miR165a ... the spatial expression of WRKY53 is more restricted to hydatodes and overall expression levels appear to be much lower in leaf tissue
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed ArticleAT5G47180 ... Expression of the third group of genes is also altered at various time points in ... rev5 wrky53 mutants compared with Col-0, but in all lines the transcriptional increase during senescence is diminished
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed ArticlemRNA expression levels of ... SAG13 ... were significantly reduced at the late developmental stages in wrky53
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed Articlequantitative PCR analysis revealed a strong induction of WRKY53 in response to H2O2 application in Col-0 wild-type plants. These changes of WRKY53 mRNA levels were significantly dampened in ... plants with reduced HD-ZIPIII activity (35S::ZPR3)
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed ArticleREV ... expressed in young seedlings
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed Articlequantitative PCR analysis revealed a strong induction of WRKY53 in response to H2O2 application in Col-0 wild-type plants. These changes of WRKY53 mRNA levels were significantly dampened in ... 35S::miR165a
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed ArticleREV is a positive regulator of WRKY53 expression and is required for high level of WRKY53 induction in response to oxidative stress. This could be either due to an upregulation of REV mRNA in response to oxidative stress or to a response of the REV protein to altered redox conditions. To test whether REV mRNA is upregulated in response to hydrogen peroxide treatment, we treated Col-0 wild-type plants with H2O2 and performed quantitative RT-PCRs. We detected no induction of REV mRNA but a slight decrease in response to high levels of hydrogen peroxide (supplementary material Fig. S4), excluding the idea that REV is transcriptionally upregulated in response to oxidative stress
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed ArticleAT5G47180 ... Expression of the third group of genes is also altered at various time points in ... rev5 ... compared with Col-0, but in all lines the transcriptional increase during senescence is diminished
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed ArticleAT1G74940 ... detected elevated levels in ... rev5 ... at early developmental stages (weeks 4 and 5) and decreased levels at the late stages
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed Articlequantitative PCR analysis revealed a strong induction of WRKY53 in response to H2O2 application in Col-0 wild-type plants
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed ArticleA genome-wide survey with a high temporal resolution classified thousands of genes as differentially expressed senescence genes (DESGs) (Breeze et al., 2011). Interestingly, REV was also classified as a DESG, showing a dip of expression at the onset of leaf senescence
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed ArticlemRNA expression levels of ... SAG13 ... were significantly reduced at the late developmental stages in ... rev5 wrky53 mutants
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed ArticleZFP8 ... Expression of the third group of genes is also altered at various time points in ... rev5 wrky53 mutants compared with Col-0, but in all lines the transcriptional increase during senescence is diminished
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed Articleseedlings carrying mutations in REV and plants with globally reduced HD-ZIPIII activity show reduced levels of WRKY53 mRNA
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed ArticleAT5G47180 ... Expression of the third group of genes is also altered at various time points in ... rev5 ... compared with Col-0, but in all lines the transcriptional increase during senescence is diminished
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed ArticleIn genetic backgrounds with reduced REV mRNA ... rev-5 ... the spatial expression of WRKY53 is more restricted to hydatodes and overall expression levels appear to be much lower in leaf tissue
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed ArticleHAT3 ... we found that expression in ... rev5 wrky53 mutants is maintained at a higher level towards the onset of senescence (weeks 5 and 6), whereas expression levels are dropping rapidly in wild-type plants
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed Articlegenetic backgrounds ... with reduced REV protein activity ... 35S::ZPR3 ... the spatial expression of WRKY53 is more restricted to hydatodes and overall expression levels appear to be much lower in leaf tissue
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed ArticleAT1G49200 ... we found that expression in ... rev5 ... is maintained at a higher level towards the onset of senescence (weeks 5 and 6), whereas expression levels are dropping rapidly in wild-type plants
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed ArticlemRNA expression levels of ... SAG13 ... were significantly reduced at the late developmental stages in ... rev5
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed ArticlemRNA expression levels of SENESCENCE ASSOCIATED GENE 12 (SAG12) ... were significantly reduced at the late developmental stages in ... rev5 wrky53 mutants
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed ArticleIDD11 ... we detected elevated levels in ... rev5 ... at early developmental stages (weeks 4 and 5) and decreased levels at the late stages
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed ArticleAT1G49200 ... we found that expression in wrky53 ... is maintained at a higher level towards the onset of senescence (weeks 5 and 6), whereas expression levels are dropping rapidly in wild-type plants
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed ArticleUsing publicly available microarray data (http://bar.utoronto.ca), we also analyzed at which stages of development and in response to which treatments REV and WRKY53 are co-expressed (supplementary material Fig. S2). We find evidence for co-expression during early developmental stages but not during the later stages of leaf development. This discrepancy suggests that REV mRNA is not upregulated at late stages of leaf development but residual protein could respond to a cellular signal and induce the expression of REV-regulated senescence targets. However, our GUS expression analyses using REV::GUS plants indicate that REV is still expressed to certain extends in older leaves
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed ArticleIDD11 ... we detected elevated levels in wrky53 ... at early developmental stages (weeks 4 and 5) and decreased levels at the late stages
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed ArticleAT1G74940 ... we detected elevated levels in ... rev5 wrky53 mutants at early developmental stages (weeks 4 and 5) and decreased levels at the late stages
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed ArticleWRKY53 shows a broader less-specific pattern of expression and is most highly expressed in old leaves
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed ArticleWRKY53 ... expressed in young seedlings
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed ArticleIDD11 ... we detected elevated levels in ... rev5 wrky53 mutants at early developmental stages (weeks 4 and 5) and decreased levels at the late stages
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed ArticleZFP8 ... Expression of the third group of genes is also altered at various time points in wrky53 ... compared with Col-0, but in all lines the transcriptional increase during senescence is diminished
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed ArticleHAT3 ... we found that expression in wrky53 ... is maintained at a higher level towards the onset of senescence (weeks 5 and 6), whereas expression levels are dropping rapidly in wild-type plants
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed ArticlemRNA expression levels of SENESCENCE ASSOCIATED GENE 12 (SAG12) ... were significantly reduced at the late developmental stages in wrky53
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed ArticleAT1G49200 ... we found that expression in ... rev5 wrky53 mutants is maintained at a higher level towards the onset of senescence (weeks 5 and 6), whereas expression levels are dropping rapidly in wild-type plants
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed Articlequantitative PCR analysis revealed a strong induction of WRKY53 in response to H2O2 application in Col-0 wild-type plants. These changes of WRKY53 mRNA levels were significantly dampened in rev mutant plants (rev-5)
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed ArticleZFP8 ... Expression of the third group of genes is also altered at various time points in ... rev5 ... compared with Col-0, but in all lines the transcriptional increase during senescence is diminished
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed ArticleAT1G74940 ... we detected elevated levels in wrky53 ... at early developmental stages (weeks 4 and 5) and decreased levels at the late stages
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed ArticleHAT3 ... we found that expression in ... rev5 ... maintained at a higher level towards the onset of senescence (weeks 5 and 6), whereas expression levels are dropping rapidly in wild-type plants
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed ArticlemRNA expression levels of SENESCENCE ASSOCIATED GENE 12 (SAG12) ... were significantly reduced at the late developmental stages in ... rev5
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed Articlevascular expression pattern of REV
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed ArticleAT5G47180 ... Expression of the third group of genes is also altered at various time points in wrky53 ... compared with Col-0, but in all lines the transcriptional increase during senescence is diminished
Xie Y, Huhn K, Brandt R, Potschin M, Bieker S, Straub D, Doll J, Drechsler T, Zentgraf U, Wenkel S - REVOLUTA and WRKY53 connect early and late leaf development in Arabidopsis
Visit the PubMed ArticlePGM2 ... cytosolic isoforms
Malinova I, Kunz HH, Alseekh S, Herbst K, Fernie AR, Gierth M, Fettke J - Reduction of the cytosolic phosphoglucomutase in Arabidopsis reveals impact on plant growth, seed and root development, and carbohydrate partitioning
Visit the PubMed Articlein pgm2/3 lines. In contrast PGM1 was somewhat up-regulated
Malinova I, Kunz HH, Alseekh S, Herbst K, Fernie AR, Gierth M, Fettke J - Reduction of the cytosolic phosphoglucomutase in Arabidopsis reveals impact on plant growth, seed and root development, and carbohydrate partitioning
Visit the PubMed ArticlePGM3 ... cytosolic isoforms
Malinova I, Kunz HH, Alseekh S, Herbst K, Fernie AR, Gierth M, Fettke J - Reduction of the cytosolic phosphoglucomutase in Arabidopsis reveals impact on plant growth, seed and root development, and carbohydrate partitioning
Visit the PubMed ArticleIn native PAGE the total PGM activity was resolved in three distinct bands of activity, the fastest moving band represented the plastidial PGM (PGM1), whereas the slowest moving band represented PGM3 (At1g23190) and the intermediate band PGM2 (At1g70730).
Malinova I, Kunz HH, Alseekh S, Herbst K, Fernie AR, Gierth M, Fettke J - Reduction of the cytosolic phosphoglucomutase in Arabidopsis reveals impact on plant growth, seed and root development, and carbohydrate partitioning
Visit the PubMed ArticleIn native PAGE the total PGM activity was resolved in three distinct bands of activity, the fastest moving band represented the plastidial PGM (PGM1), whereas the slowest moving band represented PGM3 (At1g23190) and the intermediate band PGM2 (At1g70730).
Malinova I, Kunz HH, Alseekh S, Herbst K, Fernie AR, Gierth M, Fettke J - Reduction of the cytosolic phosphoglucomutase in Arabidopsis reveals impact on plant growth, seed and root development, and carbohydrate partitioning
Visit the PubMed ArticleIn native PAGE the total PGM activity was resolved in three distinct bands of activity, the fastest moving band represented the plastidial PGM (PGM1), whereas the slowest moving band represented PGM3 (At1g23190) and the intermediate band PGM2 (At1g70730).
Malinova I, Kunz HH, Alseekh S, Herbst K, Fernie AR, Gierth M, Fettke J - Reduction of the cytosolic phosphoglucomutase in Arabidopsis reveals impact on plant growth, seed and root development, and carbohydrate partitioning
Visit the PubMed ArticleOSR2 ... epi-brassinolide ... induction was disrupted in the BR signaling mutants ... bin2
Qin Z, Zhang X, Zhang X, Feng G, Hu Y - The Arabidopsis ORGAN SIZE RELATED 2 is involved in regulation of cell expansion during organ growth
Visit the PubMed ArticleWe then examined the tissue-specific expression of OSR2 using the T3 independent transgenic plants carrying an OSR2 promoter:β-glucuronidase (proOSR2:GUS) construct ... Careful examination of GUS expression in developing first leaves showed that weak GUS signal was detected at the juvenile stage, while abundant GUS staining was observed when a leaf was undergoing expanding growth. Subsequently, the GUS signal was almost disappeared in fully-expanded leaves
Qin Z, Zhang X, Zhang X, Feng G, Hu Y - The Arabidopsis ORGAN SIZE RELATED 2 is involved in regulation of cell expansion during organ growth
Visit the PubMed ArticleWe then examined the tissue-specific expression of OSR2 using the T3 independent transgenic plants carrying an OSR2 promoter:β-glucuronidase (proOSR2:GUS) construct ... weak or nearly absent GUS signals were observed in juvenile leaves
Qin Z, Zhang X, Zhang X, Feng G, Hu Y - The Arabidopsis ORGAN SIZE RELATED 2 is involved in regulation of cell expansion during organ growth
Visit the PubMed ArticleAs the three previously-identified OSR genes are known to be differentially responsive to various hormones [17],[23],[31], we also investigated the transcriptional regulation of OSR2 by plant hormones. Interestingly, the qRT-PCR analysis of seedlings treated with various hormones revealed that, similar to ARL, OSR2 was induced by epi-brassinolide
Qin Z, Zhang X, Zhang X, Feng G, Hu Y - The Arabidopsis ORGAN SIZE RELATED 2 is involved in regulation of cell expansion during organ growth
Visit the PubMed ArticleWe then examined the tissue-specific expression of OSR2 using the T3 independent transgenic plants carrying an OSR2 promoter:β-glucuronidase (proOSR2:GUS) construct. In two-week-old seedlings ... abundant expression was also detected in the cotyledons
Qin Z, Zhang X, Zhang X, Feng G, Hu Y - The Arabidopsis ORGAN SIZE RELATED 2 is involved in regulation of cell expansion during organ growth
Visit the PubMed ArticleWe then examined the tissue-specific expression of OSR2 using the T3 independent transgenic plants carrying an OSR2 promoter:β-glucuronidase (proOSR2:GUS) construct. In two-week-old seedlings, high GUS expression was observed in the organs or organ regions where active cell expansion and elongation were occurring, such as the upper parts of leaves
Qin Z, Zhang X, Zhang X, Feng G, Hu Y - The Arabidopsis ORGAN SIZE RELATED 2 is involved in regulation of cell expansion during organ growth
Visit the PubMed ArticleTo explore whether or not OSR2 is functional during organogenesis, we initially monitored OSR2 expression in various organs with qRT-PCR analysis. As shown in Figure 1A, OSR2 was expressed primarily in the expanding organs, including leaf, inflorescence, and flower
Qin Z, Zhang X, Zhang X, Feng G, Hu Y - The Arabidopsis ORGAN SIZE RELATED 2 is involved in regulation of cell expansion during organ growth
Visit the PubMed ArticleOSR2 ... epi-brassinolide ... induction was disrupted in the BR signaling mutants bri1-6
Qin Z, Zhang X, Zhang X, Feng G, Hu Y - The Arabidopsis ORGAN SIZE RELATED 2 is involved in regulation of cell expansion during organ growth
Visit the PubMed ArticleWe then examined the tissue-specific expression of OSR2 using the T3 independent transgenic plants carrying an OSR2 promoter:β-glucuronidase (proOSR2:GUS) construct ... weak or nearly absent GUS signals were observed in ... shoot
Qin Z, Zhang X, Zhang X, Feng G, Hu Y - The Arabidopsis ORGAN SIZE RELATED 2 is involved in regulation of cell expansion during organ growth
Visit the PubMed ArticleSince the three previously-identified OSR members were known to be ER-localized [31], we further examined the cellular localization of OSR2 in leaf epidermal cells of proOSR2:OSR2-GFP transgenic plants. Unlike other OSR proteins, the OSR2-GFP fusion protein signal was detected in the plasma membrane (Additional file 1: Figure S1D), indicating that OSR2 is plasma membrane-localized
Qin Z, Zhang X, Zhang X, Feng G, Hu Y - The Arabidopsis ORGAN SIZE RELATED 2 is involved in regulation of cell expansion during organ growth
Visit the PubMed ArticleThe expression levels of the class-I KNOX genes in the shoot apex were lower in drl1-101 than in the WT (Fig. 6A). In particular, STM expression was undetected in drl1-101
Jun SE, Cho KH, Hwang JY, Abdel-Fattah W, Hammermeister A, Schaffrath R, Bowman JL, Kim GT - Comparative analysis of the conserved functions of Arabidopsis DRL1 and yeast KTI12
Visit the PubMed ArticleThe YAB gene family was expressed at high levels in drl1-101 mutant leaves relative to WT leaves
Jun SE, Cho KH, Hwang JY, Abdel-Fattah W, Hammermeister A, Schaffrath R, Bowman JL, Kim GT - Comparative analysis of the conserved functions of Arabidopsis DRL1 and yeast KTI12
Visit the PubMed ArticleThe KAN1 gene was also expressed at high levels in the drl1-101 mutant relative to the WT
Jun SE, Cho KH, Hwang JY, Abdel-Fattah W, Hammermeister A, Schaffrath R, Bowman JL, Kim GT - Comparative analysis of the conserved functions of Arabidopsis DRL1 and yeast KTI12
Visit the PubMed ArticleThe YAB gene family was expressed at high levels in drl1-101 mutant leaves relative to WT leaves
Jun SE, Cho KH, Hwang JY, Abdel-Fattah W, Hammermeister A, Schaffrath R, Bowman JL, Kim GT - Comparative analysis of the conserved functions of Arabidopsis DRL1 and yeast KTI12
Visit the PubMed ArticleARGOS ... not changed in the drl1-101 mutant relative to the WT
Jun SE, Cho KH, Hwang JY, Abdel-Fattah W, Hammermeister A, Schaffrath R, Bowman JL, Kim GT - Comparative analysis of the conserved functions of Arabidopsis DRL1 and yeast KTI12
Visit the PubMed ArticleKNAT6 ... The expression levels of the class-I KNOX genes in the shoot apex were lower in drl1-101 than in the WT
Jun SE, Cho KH, Hwang JY, Abdel-Fattah W, Hammermeister A, Schaffrath R, Bowman JL, Kim GT - Comparative analysis of the conserved functions of Arabidopsis DRL1 and yeast KTI12
Visit the PubMed ArticleThe YAB gene family was expressed at high levels in drl1-101 mutant leaves relative to WT leaves (Fig. 6B). The YAB2 gene exhibited the strongest expression of the YAB gene family members in drl1-101 mutant leaves
Jun SE, Cho KH, Hwang JY, Abdel-Fattah W, Hammermeister A, Schaffrath R, Bowman JL, Kim GT - Comparative analysis of the conserved functions of Arabidopsis DRL1 and yeast KTI12
Visit the PubMed ArticleANT ... not changed in the drl1-101 mutant relative to the WT
Jun SE, Cho KH, Hwang JY, Abdel-Fattah W, Hammermeister A, Schaffrath R, Bowman JL, Kim GT - Comparative analysis of the conserved functions of Arabidopsis DRL1 and yeast KTI12
Visit the PubMed ArticleCYCD3;1 expression was increased ... in the drl1-101 mutant relative to the WT
Jun SE, Cho KH, Hwang JY, Abdel-Fattah W, Hammermeister A, Schaffrath R, Bowman JL, Kim GT - Comparative analysis of the conserved functions of Arabidopsis DRL1 and yeast KTI12
Visit the PubMed ArticleKNAT2 ... The expression levels of the class-I KNOX genes in the shoot apex were lower in drl1-101 than in the WT
Jun SE, Cho KH, Hwang JY, Abdel-Fattah W, Hammermeister A, Schaffrath R, Bowman JL, Kim GT - Comparative analysis of the conserved functions of Arabidopsis DRL1 and yeast KTI12
Visit the PubMed ArticleKNAT1 ... The expression levels of the class-I KNOX genes in the shoot apex were lower in drl1-101 than in the WT
Jun SE, Cho KH, Hwang JY, Abdel-Fattah W, Hammermeister A, Schaffrath R, Bowman JL, Kim GT - Comparative analysis of the conserved functions of Arabidopsis DRL1 and yeast KTI12
Visit the PubMed Articlethe expression of ... nad3 ... was not affected in the slo3 mutant
Hsieh WY, Liao JC, Chang CY, Harrison T, Boucher C, Hsieh MH - The SLOW GROWTH3 Pentatricopeptide Repeat Protein Is Required for the Splicing of Mitochondrial NADH Dehydrogenase Subunit7 Intron 2 in Arabidopsis
Visit the PubMed Articlethe expression of ... nad4 ... was not affected in the slo3 mutant
Hsieh WY, Liao JC, Chang CY, Harrison T, Boucher C, Hsieh MH - The SLOW GROWTH3 Pentatricopeptide Repeat Protein Is Required for the Splicing of Mitochondrial NADH Dehydrogenase Subunit7 Intron 2 in Arabidopsis
Visit the PubMed ArticleWe showed that the 35:SLO3-GFP construct was able to complement the slo3 mutant (Fig. 4A), indicating that the SLO3-GFP fusion protein is functional in planta. Confocal microscopy was used to observe the GFP fluorescent signals in the roots of these complemented plants. MitoTracker orange was used to stain the roots as a control for mitochondrial localization. In the root hair of a 35:SLO3-GFP-complemented line, the green fluorescent signals colocalized with the MitoTracker orange fluorescent signals (Fig. 4B). Similar results were observed in root epidermal cells ... These results suggest that the SLO3-GFP fusion protein is localized to the mitochondrion
Hsieh WY, Liao JC, Chang CY, Harrison T, Boucher C, Hsieh MH - The SLOW GROWTH3 Pentatricopeptide Repeat Protein Is Required for the Splicing of Mitochondrial NADH Dehydrogenase Subunit7 Intron 2 in Arabidopsis
Visit the PubMed ArticleThe Arabidopsis nad7 gene contains four introns, and the length of intron 2 is 1,063 bp. RT-PCR analysis with primers that would amplify the 1,185-bp full-length nad7 cDNA in the wild type revealed that the majority of nad7 transcripts in the slo3 mutant were about 2.2 kb (Fig. 6A). Sequencing analysis confirmed that the 2.2-kb products were the unspliced form of the nad7 transcript retaining the entire intron 2 (Fig. 6B). By contrast, fully processed nad7 transcripts were the predominant forms present in wild-type and complemented plants (Fig. 6, A and B). These results further confirmed that the splicing of nad7 intron 2 was severely impaired in the slo3 mutant. Sequencing analysis of these RT-PCR products also allowed us to compare the editing status of fully processed and intron 2-containing nad7 transcripts in the wild type and slo3, respectively. The editing of all sites located in the exons of nad7 was not affected in the slo3 mutant (Fig. 6B; data not shown). It has been shown that RNA editing in trans-introns is involved in the splicing reaction in wheat (Triticum aestivum) mitochondria (Farré et al., 2012). Interestingly, Arabidopsis nad7 intron 2 contains an editing site near the junction of intron 2-exon 3. Although the nad7 intron 2 is not a trans-intron, it is still of interest to know if the status of this particular editing site is affected in the slo3 mutant. We used RT-PCR to amplify the intron 2-containing nad7 transcripts in the wild type with primers located in exon 2 and exon 3 (Supplemental Fig. S5). Bulk sequencing analysis of these unspliced transcripts revealed that the C-to-U editing of the nad7 intron 2 editing site was not affected in the slo3 mutant (Supplemental Fig. S5). Thus, the lack of nad7 intron 2 splicing was not due to the absence of an RNA editing factor rather than a splicing factor in the slo3 mutant
Hsieh WY, Liao JC, Chang CY, Harrison T, Boucher C, Hsieh MH - The SLOW GROWTH3 Pentatricopeptide Repeat Protein Is Required for the Splicing of Mitochondrial NADH Dehydrogenase Subunit7 Intron 2 in Arabidopsis
Visit the PubMed Articlethe expression of ... nad6 ... was not affected in the slo3 mutant
Hsieh WY, Liao JC, Chang CY, Harrison T, Boucher C, Hsieh MH - The SLOW GROWTH3 Pentatricopeptide Repeat Protein Is Required for the Splicing of Mitochondrial NADH Dehydrogenase Subunit7 Intron 2 in Arabidopsis
Visit the PubMed ArticleFor RNA splicing analysis, we used quantitative RT-PCR assays to examine the ratio of spliced to unspliced RNA for each mitochondrial intron in wild-type, slo3, and 35S:SLO3-GFP-complemented plants. Of the 23 introns examined, the splicing efficiency of nad7 intron 2 was dramatically decreased in the slo3 mutant, which was restored in the complemented plants (Fig. 5A). The quantitative RT-PCR results also revealed that the reduced slo3/wild-type ratio for nad7 intron 2 splicing was due to both an increase in unspliced and a decrease in spliced nad7 transcripts in the slo3 mutant
Hsieh WY, Liao JC, Chang CY, Harrison T, Boucher C, Hsieh MH - The SLOW GROWTH3 Pentatricopeptide Repeat Protein Is Required for the Splicing of Mitochondrial NADH Dehydrogenase Subunit7 Intron 2 in Arabidopsis
Visit the PubMed Articlethe expression of ... nad4L ... was not affected in the slo3 mutant
Hsieh WY, Liao JC, Chang CY, Harrison T, Boucher C, Hsieh MH - The SLOW GROWTH3 Pentatricopeptide Repeat Protein Is Required for the Splicing of Mitochondrial NADH Dehydrogenase Subunit7 Intron 2 in Arabidopsis
Visit the PubMed Articlethe expression of ... nad9 was not affected in the slo3 mutant
Hsieh WY, Liao JC, Chang CY, Harrison T, Boucher C, Hsieh MH - The SLOW GROWTH3 Pentatricopeptide Repeat Protein Is Required for the Splicing of Mitochondrial NADH Dehydrogenase Subunit7 Intron 2 in Arabidopsis
Visit the PubMed Articlethe expression of ... nad2 ... was not affected in the slo3 mutant
Hsieh WY, Liao JC, Chang CY, Harrison T, Boucher C, Hsieh MH - The SLOW GROWTH3 Pentatricopeptide Repeat Protein Is Required for the Splicing of Mitochondrial NADH Dehydrogenase Subunit7 Intron 2 in Arabidopsis
Visit the PubMed Articlethe expression of nad1 ... was not affected in the slo3 mutant
Hsieh WY, Liao JC, Chang CY, Harrison T, Boucher C, Hsieh MH - The SLOW GROWTH3 Pentatricopeptide Repeat Protein Is Required for the Splicing of Mitochondrial NADH Dehydrogenase Subunit7 Intron 2 in Arabidopsis
Visit the PubMed Articlethe expression of ... nad5 ... was not affected in the slo3 mutant
Hsieh WY, Liao JC, Chang CY, Harrison T, Boucher C, Hsieh MH - The SLOW GROWTH3 Pentatricopeptide Repeat Protein Is Required for the Splicing of Mitochondrial NADH Dehydrogenase Subunit7 Intron 2 in Arabidopsis
Visit the PubMed ArticleANT ... expression was not detected in mature rosette leaf (Figure 76), young rosette, or cauline leaf tissue (data not shown)
Klucher KM, Chow H, Reiser L, Fischer RL - The AINTEGUMENTA gene of Arabidopsis required for ovule and female gametophyte development is related to the floral homeotic gene APETALA2
Visit the PubMed ArticleLater, at the torpedo stage, ANT is expressed in cells in an internal zone extending the length of each cotyledon (Figure 7N). These may include procambial cells that will ultimately give rise to the vasculature. The embryonic hypocotyl and root show no labeling.
Elliott RC, Betzner AS, Huttner E, Oakes MP, Tucker WQ, Gerentes D, Perez P, Smyth DR - AINTEGUMENTA, an APETALA2-like gene of Arabidopsis with pleiotropic roles in ovule development and floral organ growth
Visit the PubMed ArticleAfter fertilization, ANT expression shifts to the embryo. There appear to be two regions of expression in globular-stage embryos, presumably in cells that will give rise to the cotyledons
Elliott RC, Betzner AS, Huttner E, Oakes MP, Tucker WQ, Gerentes D, Perez P, Smyth DR - AINTEGUMENTA, an APETALA2-like gene of Arabidopsis with pleiotropic roles in ovule development and floral organ growth
Visit the PubMed ArticleANT is expressed ... in the primordia of ... cotyledons
Elliott RC, Betzner AS, Huttner E, Oakes MP, Tucker WQ, Gerentes D, Perez P, Smyth DR - AINTEGUMENTA, an APETALA2-like gene of Arabidopsis with pleiotropic roles in ovule development and floral organ growth
Visit the PubMed ArticleWhen ANT expression was examined ... developing leaves contained two internal zones of expression extending longitudinally down each side (Figure 7P), very similar to that seen in developing sepals (Figure 7D
Elliott RC, Betzner AS, Huttner E, Oakes MP, Tucker WQ, Gerentes D, Perez P, Smyth DR - AINTEGUMENTA, an APETALA2-like gene of Arabidopsis with pleiotropic roles in ovule development and floral organ growth
Visit the PubMed ArticleRoot meristems showed no evidence of ANT expression, and neither did mature tissues of roots, stems, and leaves
Elliott RC, Betzner AS, Huttner E, Oakes MP, Tucker WQ, Gerentes D, Perez P, Smyth DR - AINTEGUMENTA, an APETALA2-like gene of Arabidopsis with pleiotropic roles in ovule development and floral organ growth
Visit the PubMed ArticleWhen ANT expression was examined in young seedlings at the two-leaf stage, the shoot apical meristem contained discrete zones of labeling in its periphery, presumably indicating where leaf primordia would ultimately arise (Figure 70
Elliott RC, Betzner AS, Huttner E, Oakes MP, Tucker WQ, Gerentes D, Perez P, Smyth DR - AINTEGUMENTA, an APETALA2-like gene of Arabidopsis with pleiotropic roles in ovule development and floral organ growth
Visit the PubMed ArticleANT is expressed ... in the primordia of ... leaves
Elliott RC, Betzner AS, Huttner E, Oakes MP, Tucker WQ, Gerentes D, Perez P, Smyth DR - AINTEGUMENTA, an APETALA2-like gene of Arabidopsis with pleiotropic roles in ovule development and floral organ growth
Visit the PubMed ArticleThe strain #1-35-38 ... was specific to ... true (foliage) leaves
Tsukaya H, Uchimiya H - Genetic analyses of the formation of the serrated margin of leaf blades in Arabidopsis: combination of a mutational analysis of leaf morphogenesis with the characterization of a specific marker gene expressed in hydathodes and stipules
Visit the PubMed ArticleThe strain #1-35-38, which expresses the marker gene for GUS ... in stipules
Tsukaya H, Uchimiya H - Genetic analyses of the formation of the serrated margin of leaf blades in Arabidopsis: combination of a mutational analysis of leaf morphogenesis with the characterization of a specific marker gene expressed in hydathodes and stipules
Visit the PubMed ArticleThe strain #1-35-38 ... was specific to cotyledons
Tsukaya H, Uchimiya H - Genetic analyses of the formation of the serrated margin of leaf blades in Arabidopsis: combination of a mutational analysis of leaf morphogenesis with the characterization of a specific marker gene expressed in hydathodes and stipules
Visit the PubMed ArticleThe strain #1-35-38, which expresses the marker gene for GUS ... in ... hydathodes
Tsukaya H, Uchimiya H - Genetic analyses of the formation of the serrated margin of leaf blades in Arabidopsis: combination of a mutational analysis of leaf morphogenesis with the characterization of a specific marker gene expressed in hydathodes and stipules
Visit the PubMed ArticleAGO1 mRNA was detected in all tissues examined
Bohmert K, Camus I, Bellini C, Bouchez D, Caboche M, Benning C - AGO1 defines a novel locus of Arabidopsis controlling leaf development
Visit the PubMed ArticleROT3 is expressed in all major or- gans, especially in tissues that have high activity of cell division
Kim GT, Tsukaya H, Uchimiya H - The ROTUNDIFOLIA3 gene of Arabidopsis thaliana encodes a new member of the cytochrome P-450 family that is required for the regulated polar elongation of leaf cells
Visit the PubMed ArticlePNH ... Similar to the embryonic pattern, highlevel expression is detected in developing vascular strands
Lynn K, Fernandez A, Aida M, Sedbrook J, Tasaka M, Masson P, Barton MK - The PINHEAD/ZWILLE gene acts pleiotropically in Arabidopsis development and has overlapping functions with the ARGONAUTE1 gene
Visit the PubMed ArticlePNH ... At torpedo stage, the dark stain in the provascular cells expands up the center of the cotyledons. Light staining appears only adaxial to this stripe of expression in the cotyledons and is still in the SAM and root pole (Fig. 3F). A transverse section through the cotyledon primordia at this stage demonstrates differential expression between their ad- and abaxial sides (Fig. 3G). This pattern is stably maintained through the walking stick stage (Fig. 3H). Transverse sections through a nearly mature embryo reveal dark staining in the procambium of the root-hypocotyl axis, lighter staining in the primary xylem precursor area and a pattern of dark staining in the cotyledons that corresponds to the pattern of vascular strands in the mature cotyledon (Fig. 3I)
Lynn K, Fernandez A, Aida M, Sedbrook J, Tasaka M, Masson P, Barton MK - The PINHEAD/ZWILLE gene acts pleiotropically in Arabidopsis development and has overlapping functions with the ARGONAUTE1 gene
Visit the PubMed ArticlePNH ... low-level expression is detected in the SAM
Lynn K, Fernandez A, Aida M, Sedbrook J, Tasaka M, Masson P, Barton MK - The PINHEAD/ZWILLE gene acts pleiotropically in Arabidopsis development and has overlapping functions with the ARGONAUTE1 gene
Visit the PubMed ArticleAGO1 ... A cross-section through the cotyledons of a torpedo stage embryo demonstrates that, unlike PNH mRNA, AGO1 mRNA does not accumulate differentially in the adaxial regions of the cotyledons (Fig. 5E
Lynn K, Fernandez A, Aida M, Sedbrook J, Tasaka M, Masson P, Barton MK - The PINHEAD/ZWILLE gene acts pleiotropically in Arabidopsis development and has overlapping functions with the ARGONAUTE1 gene
Visit the PubMed ArticleWhile p0 expresses PNH uniformly (Fig. 4E,F), p1 exhibits some differential expression of PNH along the ad/abaxial dimension (Fig. 4B,D,H) indicating that by the p1 stage the primordium has acquired polarity in the radial dimension. By the p2 stage, outgrowth from the SAM is conspicuous and differential PNH expression is more distinct
Lynn K, Fernandez A, Aida M, Sedbrook J, Tasaka M, Masson P, Barton MK - The PINHEAD/ZWILLE gene acts pleiotropically in Arabidopsis development and has overlapping functions with the ARGONAUTE1 gene
Visit the PubMed ArticleAfter germination, PNH expression persists in the vegetative meristem
Lynn K, Fernandez A, Aida M, Sedbrook J, Tasaka M, Masson P, Barton MK - The PINHEAD/ZWILLE gene acts pleiotropically in Arabidopsis development and has overlapping functions with the ARGONAUTE1 gene
Visit the PubMed ArticleThe AGO1 transcript is expressed as early as the globular stage (Fig. 5A). While PNH mRNA accumulation is detectable at two levels, AGO1 message is evenly distributed throughout the embryo proper and is present in the suspensor (Fig. 5B). This pattern continues throughout embryogenesis (Fig. 5B-D)
Lynn K, Fernandez A, Aida M, Sedbrook J, Tasaka M, Masson P, Barton MK - The PINHEAD/ZWILLE gene acts pleiotropically in Arabidopsis development and has overlapping functions with the ARGONAUTE1 gene
Visit the PubMed ArticlePNH ... low-level expression is detected in the ... adaxial leaf domains
Lynn K, Fernandez A, Aida M, Sedbrook J, Tasaka M, Masson P, Barton MK - The PINHEAD/ZWILLE gene acts pleiotropically in Arabidopsis development and has overlapping functions with the ARGONAUTE1 gene
Visit the PubMed Articlein seedlings the AGO1 transcript is evenly distributed throughout the ... leaf primordia
Lynn K, Fernandez A, Aida M, Sedbrook J, Tasaka M, Masson P, Barton MK - The PINHEAD/ZWILLE gene acts pleiotropically in Arabidopsis development and has overlapping functions with the ARGONAUTE1 gene
Visit the PubMed ArticlePNH ... At the globular stage, the provascular cells stain intensely, while the protoderm and lenticular cell stain lightly and no stain is detected in the lower protoderm
Lynn K, Fernandez A, Aida M, Sedbrook J, Tasaka M, Masson P, Barton MK - The PINHEAD/ZWILLE gene acts pleiotropically in Arabidopsis development and has overlapping functions with the ARGONAUTE1 gene
Visit the PubMed ArticlePNH ... By early heart stage, the lighter stain spans the cells expected to give rise to the adaxial sides of the cotyledons and the SAM, and is in the derivatives of the lenticular cell in the root pole (Fig. 3E). The boundary between lightly staining and non-staining cells bisects the cotyledon primordia along their ad/abaxial axis (Fig. 3E-G, arrows
Lynn K, Fernandez A, Aida M, Sedbrook J, Tasaka M, Masson P, Barton MK - The PINHEAD/ZWILLE gene acts pleiotropically in Arabidopsis development and has overlapping functions with the ARGONAUTE1 gene
Visit the PubMed Articlein seedlings the AGO1 transcript is evenly distributed throughout the SAM
Lynn K, Fernandez A, Aida M, Sedbrook J, Tasaka M, Masson P, Barton MK - The PINHEAD/ZWILLE gene acts pleiotropically in Arabidopsis development and has overlapping functions with the ARGONAUTE1 gene
Visit the PubMed ArticlePNH mRNA accumulation was examined on a northern blot using the pKL3 probe, which detects a single 3.6 kb transcript in roots, stems, leaves, siliques and inflorescences (data not shown
Lynn K, Fernandez A, Aida M, Sedbrook J, Tasaka M, Masson P, Barton MK - The PINHEAD/ZWILLE gene acts pleiotropically in Arabidopsis development and has overlapping functions with the ARGONAUTE1 gene
Visit the PubMed ArticlePNH message is evenly distributed throughout the embryo proper until the dermatogen stage, when staining appears lighter in the protoderm (Fig. 3B).
Lynn K, Fernandez A, Aida M, Sedbrook J, Tasaka M, Masson P, Barton MK - The PINHEAD/ZWILLE gene acts pleiotropically in Arabidopsis development and has overlapping functions with the ARGONAUTE1 gene
Visit the PubMed ArticleIn the embryo, PNH mRNA is detected by the four-cell stage when accumulation is found in the embryo proper and there is light staining in the uppermost cell of the suspensor
Lynn K, Fernandez A, Aida M, Sedbrook J, Tasaka M, Masson P, Barton MK - The PINHEAD/ZWILLE gene acts pleiotropically in Arabidopsis development and has overlapping functions with the ARGONAUTE1 gene
Visit the PubMed Article